<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:01a0582c-f590-78e6-a0fd-8206cdef44e0,g:099252c6-d99c-456a-a902-81378aebf933-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.jbssa.com/role/Cover" xlink:type="simple" xlink:href="jbsay-20260930.xsd#Cover"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationTable_01a0582c-fa68-74a5-90a8-f545def505d6" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentInformationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentInformationTable_01a0582c-fa68-74a5-90a8-f545def505d6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalAxis_01a0582c-fa68-78fd-8ead-3fad805f7a9f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfShareCapitalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_01a0582c-fa68-74a5-90a8-f545def505d6" xlink:to="loc_ifrs-full_ClassesOfShareCapitalAxis_01a0582c-fa68-78fd-8ead-3fad805f7a9f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_01a0582c-fa68-78fd-8ead-3fad805f7a9f_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_01a0582c-fa68-78fd-8ead-3fad805f7a9f" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_01a0582c-fa68-78fd-8ead-3fad805f7a9f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_01a0582c-fa68-752d-803e-b09f31cb7f58" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_01a0582c-fa68-78fd-8ead-3fad805f7a9f" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_01a0582c-fa68-752d-803e-b09f31cb7f58" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ClassACommonStockMember_01a0582c-fa68-7de7-ae44-f13627b9650c" xlink:href="jbsay-20260930.xsd#jbsay_ClassACommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_01a0582c-fa68-752d-803e-b09f31cb7f58" xlink:to="loc_jbsay_ClassACommonStockMember_01a0582c-fa68-7de7-ae44-f13627b9650c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ClassBCommonStockMember_01a0582c-fa68-77bd-9d7a-d5ed00188e10" xlink:href="jbsay-20260930.xsd#jbsay_ClassBCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_01a0582c-fa68-752d-803e-b09f31cb7f58" xlink:to="loc_jbsay_ClassBCommonStockMember_01a0582c-fa68-77bd-9d7a-d5ed00188e10" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityListingsExchangeAxis_01a0582c-fa68-78bc-bf72-224430266e8b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityListingsExchangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_01a0582c-fa68-74a5-90a8-f545def505d6" xlink:to="loc_dei_EntityListingsExchangeAxis_01a0582c-fa68-78bc-bf72-224430266e8b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_ExchangeDomain_01a0582c-fa68-78bc-bf72-224430266e8b_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_ExchangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_EntityListingsExchangeAxis_01a0582c-fa68-78bc-bf72-224430266e8b" xlink:to="loc_dei_ExchangeDomain_01a0582c-fa68-78bc-bf72-224430266e8b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_ExchangeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_EntityListingsExchangeAxis_01a0582c-fa68-78bc-bf72-224430266e8b" xlink:to="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A2.500SeniorNotesDue2027Member_01a0582c-fa68-7a52-a2e0-1da61a38b3eb" xlink:href="jbsay-20260930.xsd#jbsay_A2.500SeniorNotesDue2027Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A2.500SeniorNotesDue2027Member_01a0582c-fa68-7a52-a2e0-1da61a38b3eb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A3.000SeniorNotesDue2029Member_01a0582c-fa68-7b5f-ac37-d619aa437b68" xlink:href="jbsay-20260930.xsd#jbsay_A3.000SeniorNotesDue2029Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A3.000SeniorNotesDue2029Member_01a0582c-fa68-7b5f-ac37-d619aa437b68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A3.750SeniorNotesDue2031Member_01a0582c-fa68-7091-aba9-8ca8251b9e2d" xlink:href="jbsay-20260930.xsd#jbsay_A3.750SeniorNotesDue2031Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A3.750SeniorNotesDue2031Member_01a0582c-fa68-7091-aba9-8ca8251b9e2d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A3.000SustainabilityLinkedSeniorNotesDue2032Member_01a0582c-fa68-7f96-a23e-c215511e6381" xlink:href="jbsay-20260930.xsd#jbsay_A3.000SustainabilityLinkedSeniorNotesDue2032Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A3.000SustainabilityLinkedSeniorNotesDue2032Member_01a0582c-fa68-7f96-a23e-c215511e6381" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A3.625SustainabilityLinkedSeniorNotesDue2032Member_01a0582c-fa68-7e0b-b423-4b7108b54b91" xlink:href="jbsay-20260930.xsd#jbsay_A3.625SustainabilityLinkedSeniorNotesDue2032Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A3.625SustainabilityLinkedSeniorNotesDue2032Member_01a0582c-fa68-7e0b-b423-4b7108b54b91" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A5.750SeniorNotesDue2033Member_01a0582c-fa68-7df5-88d2-0ab4e59105b4" xlink:href="jbsay-20260930.xsd#jbsay_A5.750SeniorNotesDue2033Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A5.750SeniorNotesDue2033Member_01a0582c-fa68-7df5-88d2-0ab4e59105b4" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A6.750SeniorNotesDue2034Member_01a0582c-fa68-7ed1-a65d-61b31f671e9f" xlink:href="jbsay-20260930.xsd#jbsay_A6.750SeniorNotesDue2034Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A6.750SeniorNotesDue2034Member_01a0582c-fa68-7ed1-a65d-61b31f671e9f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A5.950SeniorNotesDue2035Member_01a0582c-fa68-72e6-9ad9-b88cd3fc2baf" xlink:href="jbsay-20260930.xsd#jbsay_A5.950SeniorNotesDue2035Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A5.950SeniorNotesDue2035Member_01a0582c-fa68-72e6-9ad9-b88cd3fc2baf" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A5.500SeniorNotesDue2036Member_01a0582c-fa68-7d9a-877d-500ab15abba7" xlink:href="jbsay-20260930.xsd#jbsay_A5.500SeniorNotesDue2036Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A5.500SeniorNotesDue2036Member_01a0582c-fa68-7d9a-877d-500ab15abba7" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A4.375SeniorNotesDue2052Member_01a0582c-fa68-75b0-86b8-a148b92c44a5" xlink:href="jbsay-20260930.xsd#jbsay_A4.375SeniorNotesDue2052Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A4.375SeniorNotesDue2052Member_01a0582c-fa68-75b0-86b8-a148b92c44a5" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A6.500SeniorNotesDue2052Member_01a0582c-fa68-7bf4-8d8c-2790677e524f" xlink:href="jbsay-20260930.xsd#jbsay_A6.500SeniorNotesDue2052Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A6.500SeniorNotesDue2052Member_01a0582c-fa68-7bf4-8d8c-2790677e524f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A7.250SeniorNotesDue2053Member_01a0582c-fa68-77eb-8da5-d35b9425d97d" xlink:href="jbsay-20260930.xsd#jbsay_A7.250SeniorNotesDue2053Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A7.250SeniorNotesDue2053Member_01a0582c-fa68-77eb-8da5-d35b9425d97d" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A6.375SeniorNotesDue2055Member_01a0582c-fa68-79e8-8655-4f93970f12a8" xlink:href="jbsay-20260930.xsd#jbsay_A6.375SeniorNotesDue2055Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A6.375SeniorNotesDue2055Member_01a0582c-fa68-79e8-8655-4f93970f12a8" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A6.250SeniorNotesDue2056Member_01a0582c-fa68-79a3-88f6-3e4edaafc143" xlink:href="jbsay-20260930.xsd#jbsay_A6.250SeniorNotesDue2056Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A6.250SeniorNotesDue2056Member_01a0582c-fa68-79a3-88f6-3e4edaafc143" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_A6.375SeniorNotesDue2066Member_01a0582c-fa68-76a0-982f-fb01693a70e5" xlink:href="jbsay-20260930.xsd#jbsay_A6.375SeniorNotesDue2066Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_ExchangeDomain_01a0582c-fa68-721f-bf52-f5e0672045c7" xlink:to="loc_jbsay_A6.375SeniorNotesDue2066Member_01a0582c-fa68-76a0-982f-fb01693a70e5" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_01a0582c-fa68-7f1c-8474-fde987522e1e" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentType_01a0582c-fa68-7f1c-8474-fde987522e1e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentQuarterlyReport_01a0582c-fa68-7695-a557-3fd46c70dd83" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentQuarterlyReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentQuarterlyReport_01a0582c-fa68-7695-a557-3fd46c70dd83" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_01a0582c-fa68-7efc-8555-268d6a79f5d4" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentPeriodEndDate_01a0582c-fa68-7efc-8555-268d6a79f5d4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentTransitionReport_01a0582c-fa68-71d7-b84a-bc1a1bae899d" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentTransitionReport"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentTransitionReport_01a0582c-fa68-71d7-b84a-bc1a1bae899d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_01a0582c-fa68-7773-8679-23867b7501b4" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityFileNumber_01a0582c-fa68-7773-8679-23867b7501b4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_01a0582c-fa68-79ba-8ecf-b4690e274d44" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityRegistrantName_01a0582c-fa68-79ba-8ecf-b4690e274d44" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCountry_01a0582c-fa68-7606-abb9-a87f95aff361" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityAddressCountry"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityAddressCountry_01a0582c-fa68-7606-abb9-a87f95aff361" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_01a0582c-fa68-7551-810b-4ffdf3d27385" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityTaxIdentificationNumber_01a0582c-fa68-7551-810b-4ffdf3d27385" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_01a0582c-fa68-7d4b-a65d-798f791dc27e" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityAddressAddressLine1_01a0582c-fa68-7d4b-a65d-798f791dc27e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_01a0582c-fa68-7307-94f3-3f276bdc13d7" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityAddressCityOrTown_01a0582c-fa68-7307-94f3-3f276bdc13d7" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_01a0582c-fa68-770e-91f7-cf94d8a78f88" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityAddressPostalZipCode_01a0582c-fa68-770e-91f7-cf94d8a78f88" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_01a0582c-fa68-7b97-9da9-4ee0252ef676" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_CityAreaCode_01a0582c-fa68-7b97-9da9-4ee0252ef676" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_01a0582c-fa68-7a02-be3b-3dd4717b717d" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_LocalPhoneNumber_01a0582c-fa68-7a02-be3b-3dd4717b717d" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_01a0582c-fa68-7a70-a748-947788df8d40" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_Security12bTitle_01a0582c-fa68-7a70-a748-947788df8d40" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_01a0582c-fa68-7239-b09d-86e071a705e4" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_TradingSymbol_01a0582c-fa68-7239-b09d-86e071a705e4" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_01a0582c-fa68-7d02-8ab0-c7c86cc08f8c" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_SecurityExchangeName_01a0582c-fa68-7d02-8ab0-c7c86cc08f8c" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCurrentReportingStatus_01a0582c-fa68-74aa-9988-37fb561f4ae6" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityCurrentReportingStatus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityCurrentReportingStatus_01a0582c-fa68-74aa-9988-37fb561f4ae6" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityInteractiveDataCurrent_01a0582c-fa68-7b42-ad97-c7254339c082" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityInteractiveDataCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityInteractiveDataCurrent_01a0582c-fa68-7b42-ad97-c7254339c082" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFilerCategory_01a0582c-fa68-71ae-9e95-89067fef31c2" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityFilerCategory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityFilerCategory_01a0582c-fa68-71ae-9e95-89067fef31c2" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntitySmallBusiness_01a0582c-fb13-7cd1-a8bd-8347f6a66203" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntitySmallBusiness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntitySmallBusiness_01a0582c-fb13-7cd1-a8bd-8347f6a66203" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_01a0582c-fb13-7e63-a8d7-a4ddc78e3a1b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityEmergingGrowthCompany_01a0582c-fb13-7e63-a8d7-a4ddc78e3a1b" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityShellCompany_01a0582c-fb13-7cf7-93bf-55c22562dc09" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityShellCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityShellCompany_01a0582c-fb13-7cf7-93bf-55c22562dc09" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCommonStockSharesOutstanding_01a0582c-fb13-7b5b-96af-c1fd4cf0067c" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityCommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityCommonStockSharesOutstanding_01a0582c-fb13-7b5b-96af-c1fd4cf0067c" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CurrentFiscalYearEndDate_01a0582c-fb13-765b-b7b4-aa62bd0775f8" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_CurrentFiscalYearEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_CurrentFiscalYearEndDate_01a0582c-fb13-765b-b7b4-aa62bd0775f8" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalPeriodFocus_01a0582c-fb13-715e-9468-9f567efb86d0" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentFiscalPeriodFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentFiscalPeriodFocus_01a0582c-fb13-715e-9468-9f567efb86d0" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_01a0582c-fb13-7d2f-87a1-a31e46da5895" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_AmendmentFlag_01a0582c-fb13-7d2f-87a1-a31e46da5895" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentFiscalYearFocus_01a0582c-fb13-76f3-bf1a-6c2138f0b301" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentFiscalYearFocus"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_DocumentFiscalYearFocus_01a0582c-fb13-76f3-bf1a-6c2138f0b301" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_01a0582c-fb13-702e-a156-5e7552392109" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityCentralIndexKey_01a0582c-fb13-702e-a156-5e7552392109" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_01a0582c-fb13-708d-97c9-324a01b5ca35" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_01a0582c-fa68-721e-9c43-a4975804a71f" xlink:to="loc_dei_EntityIncorporationStateCountryCode_01a0582c-fb13-708d-97c9-324a01b5ca35" xlink:type="arc" order="28"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/ConsolidatedStatementsofChangesinEquity" xlink:type="simple" xlink:href="jbsay-20260930.xsd#ConsolidatedStatementsofChangesinEquity"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/ConsolidatedStatementsofChangesinEquity" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_StatementOfChangesInEquityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_StatementOfChangesInEquityTable_01a1878f-445d-701b-aecd-a0513e4636d7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_StatementOfChangesInEquityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_StatementOfChangesInEquityTable_01a1878f-445d-701b-aecd-a0513e4636d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityAxis_01a1878f-445d-730b-bf51-ea735c4e2382" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_StatementOfChangesInEquityTable_01a1878f-445d-701b-aecd-a0513e4636d7" xlink:to="loc_ifrs-full_ComponentsOfEquityAxis_01a1878f-445d-730b-bf51-ea735c4e2382" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityDomain_01a1878f-445d-730b-bf51-ea735c4e2382_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ComponentsOfEquityAxis_01a1878f-445d-730b-bf51-ea735c4e2382" xlink:to="loc_ifrs-full_ComponentsOfEquityDomain_01a1878f-445d-730b-bf51-ea735c4e2382_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfEquityDomain_01a1878f-445d-7128-a9ef-d83f6a85bd9a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ComponentsOfEquityAxis_01a1878f-445d-730b-bf51-ea735c4e2382" xlink:to="loc_ifrs-full_ComponentsOfEquityDomain_01a1878f-445d-7128-a9ef-d83f6a85bd9a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EquityAttributableToOwnersOfParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_01a1878f-445d-7128-a9ef-d83f6a85bd9a" xlink:to="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ClassesOfShareCapitalMember_01a1878f-445d-7265-bba2-24e6a1218d52" xlink:href="jbsay-20260930.xsd#jbsay_ClassesOfShareCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_jbsay_ClassesOfShareCapitalMember_01a1878f-445d-7265-bba2-24e6a1218d52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SharePremiumMember_01a1878f-445d-7bf9-b456-2fa144383fdf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SharePremiumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_SharePremiumMember_01a1878f-445d-7bf9-b456-2fa144383fdf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PremiumOnIssueOfSharesMember_01a1878f-445d-7e8d-8c06-cae69a898adf" xlink:href="jbsay-20260930.xsd#jbsay_PremiumOnIssueOfSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_jbsay_PremiumOnIssueOfSharesMember_01a1878f-445d-7e8d-8c06-cae69a898adf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CapitalTransactionMember_01a1878f-445d-7c5d-8a67-e0eb5f991b6c" xlink:href="jbsay-20260930.xsd#jbsay_CapitalTransactionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_jbsay_CapitalTransactionMember_01a1878f-445d-7c5d-8a67-e0eb5f991b6c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ReserveOfChangeInValueOfTimeValueOfOptionsMember_01a1878f-445d-76f1-9f8f-b3924c600581" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ReserveOfChangeInValueOfTimeValueOfOptionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_ReserveOfChangeInValueOfTimeValueOfOptionsMember_01a1878f-445d-76f1-9f8f-b3924c600581" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherReservesMember_01a1878f-445d-77ef-b2e4-1feab67fc67b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherReservesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_OtherReservesMember_01a1878f-445d-77ef-b2e4-1feab67fc67b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TreasurySharesMember_01a1878f-445d-76e3-9eb5-d008666ff06f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TreasurySharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_TreasurySharesMember_01a1878f-445d-76e3-9eb5-d008666ff06f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LegalReserveEquityMember_01a1878f-445d-744f-a76b-ec11430ca152" xlink:href="jbsay-20260930.xsd#jbsay_LegalReserveEquityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_jbsay_LegalReserveEquityMember_01a1878f-445d-744f-a76b-ec11430ca152" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_StatutoryReserveMember_01a1878f-445d-7b9a-8c4b-de52717c9e19" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_StatutoryReserveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_StatutoryReserveMember_01a1878f-445d-7b9a-8c4b-de52717c9e19" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TaxIncentiveReserveMember_01a1878f-445d-702b-aebb-86d190f29c76" xlink:href="jbsay-20260930.xsd#jbsay_TaxIncentiveReserveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_jbsay_TaxIncentiveReserveMember_01a1878f-445d-702b-aebb-86d190f29c76" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ValuationAdjustmentsToEquityMember_01a1878f-445d-7399-9e60-1e90c52a7d59" xlink:href="jbsay-20260930.xsd#jbsay_ValuationAdjustmentsToEquityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_jbsay_ValuationAdjustmentsToEquityMember_01a1878f-445d-7399-9e60-1e90c52a7d59" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AccumulatedOtherComprehensiveIncomeMember_01a1878f-445d-796d-bd1f-bfb30a0529da" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_AccumulatedOtherComprehensiveIncomeMember_01a1878f-445d-796d-bd1f-bfb30a0529da" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RetainedEarningsMember_01a1878f-445d-79b4-b27d-55b890228dec" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAttributableToOwnersOfParentMember_01a1878f-445d-7331-b7c9-2c5ec5ca05a5" xlink:to="loc_ifrs-full_RetainedEarningsMember_01a1878f-445d-79b4-b27d-55b890228dec" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncontrollingInterestsMember_01a1878f-445d-77d3-82ce-62f1c2d8edbc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncontrollingInterestsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfEquityDomain_01a1878f-445d-7128-a9ef-d83f6a85bd9a" xlink:to="loc_ifrs-full_NoncontrollingInterestsMember_01a1878f-445d-77d3-82ce-62f1c2d8edbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsAxis_01a18794-f2c5-781c-916c-36cd12686a19" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_StatementOfChangesInEquityTable_01a1878f-445d-701b-aecd-a0513e4636d7" xlink:to="loc_ifrs-full_BusinessCombinationsAxis_01a18794-f2c5-781c-916c-36cd12686a19" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_01a18794-f2c5-781c-916c-36cd12686a19_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_01a18794-f2c5-781c-916c-36cd12686a19" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_01a18794-f2c5-781c-916c-36cd12686a19_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_01a18794-f2c5-72b5-a50d-48d35fac041b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_01a18794-f2c5-781c-916c-36cd12686a19" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_01a18794-f2c5-72b5-a50d-48d35fac041b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BaumhardtSubsidiaryMember_01a18794-f2c5-7d44-b75c-22ef7be60941" xlink:href="jbsay-20260930.xsd#jbsay_BaumhardtSubsidiaryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a18794-f2c5-72b5-a50d-48d35fac041b" xlink:to="loc_jbsay_BaumhardtSubsidiaryMember_01a18794-f2c5-7d44-b75c-22ef7be60941" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AndrewsMeatsMember_01a18794-f2c5-7495-9f62-1bc0d56e6cd8" xlink:href="jbsay-20260930.xsd#jbsay_AndrewsMeatsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a18794-f2c5-72b5-a50d-48d35fac041b" xlink:to="loc_jbsay_AndrewsMeatsMember_01a18794-f2c5-7495-9f62-1bc0d56e6cd8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Equity_01a1878f-445d-7adf-95a3-114964b721ab" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Equity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_Equity_01a1878f-445d-7adf-95a3-114964b721ab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_01a1878f-445d-7caf-98c5-49fe1b1bf107" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_ProfitLoss_01a1878f-445d-7caf-98c5-49fe1b1bf107" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnHedgesOfNetInvestmentsInForeignOperationsNetOfTax_01a1878f-445d-7977-89c0-5446d7adf128" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnHedgesOfNetInvestmentsInForeignOperationsNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_GainsLossesOnHedgesOfNetInvestmentsInForeignOperationsNetOfTax_01a1878f-445d-7977-89c0-5446d7adf128" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnCashFlowHedgesNetOfTax_01a1878f-445d-7420-9640-36dcfb748d56" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnCashFlowHedgesNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_GainsLossesOnCashFlowHedgesNetOfTax_01a1878f-445d-7420-9640-36dcfb748d56" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnChangesInEffectOfLimitingNetDefinedBenefitAssetToAssetCeilingExcludingInterestIncomeOrExpenseNetOfTaxDefinedBenefitPlans_01a1878f-445d-7f98-b2a5-622ebd9d4f11" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnChangesInEffectOfLimitingNetDefinedBenefitAssetToAssetCeilingExcludingInterestIncomeOrExpenseNetOfTaxDefinedBenefitPlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_GainsLossesOnChangesInEffectOfLimitingNetDefinedBenefitAssetToAssetCeilingExcludingInterestIncomeOrExpenseNetOfTaxDefinedBenefitPlans_01a1878f-445d-7f98-b2a5-622ebd9d4f11" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughChangeInEquityOfSubsidiaries_01a1878f-445d-7f34-9902-7f9b300e2e3d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughChangeInEquityOfSubsidiaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughChangeInEquityOfSubsidiaries_01a1878f-445d-7f34-9902-7f9b300e2e3d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ReclassificationAdjustmentsOnExchangeDifferencesOnTranslationNetOfTax_01a1878f-445d-7a8c-ae40-d11a65fded7e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ReclassificationAdjustmentsOnExchangeDifferencesOnTranslationNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_ReclassificationAdjustmentsOnExchangeDifferencesOnTranslationNetOfTax_01a1878f-445d-7a8c-ae40-d11a65fded7e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OtherFairValueAdjustmentThroughOtherComprehensiveIncome_01a1878f-445d-79fd-b6e3-7d9500e4c353" xlink:href="jbsay-20260930.xsd#jbsay_OtherFairValueAdjustmentThroughOtherComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_OtherFairValueAdjustmentThroughOtherComprehensiveIncome_01a1878f-445d-79fd-b6e3-7d9500e4c353" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComprehensiveIncome_01a1878f-445d-70b0-8d10-16699d5e139d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_ComprehensiveIncome_01a1878f-445d-70b0-8d10-16699d5e139d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CancellationOfTreasuryShares_01a1878f-445d-721d-b029-c83aa53a39a3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CancellationOfTreasuryShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_CancellationOfTreasuryShares_01a1878f-445d-721d-b029-c83aa53a39a3" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughTransferToStatutoryReserve_01a18864-402c-794b-b759-5bdef92f49ba" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughTransferToStatutoryReserve"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughTransferToStatutoryReserve_01a18864-402c-794b-b759-5bdef92f49ba" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SaleOrIssueOfTreasuryShares_01a189da-1909-7058-86a2-0b391ebdff92" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SaleOrIssueOfTreasuryShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_SaleOrIssueOfTreasuryShares_01a189da-1909-7058-86a2-0b391ebdff92" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughOtherContributionsByOwners_01a1878f-445d-7937-b3c9-ce096d352d89" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughOtherContributionsByOwners"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughOtherContributionsByOwners_01a1878f-445d-7937-b3c9-ce096d352d89" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncorporationOfSharesEquity_01a1878f-445d-730a-a1f7-ac3bb9fe3b40" xlink:href="jbsay-20260930.xsd#jbsay_IncorporationOfSharesEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_IncorporationOfSharesEquity_01a1878f-445d-730a-a1f7-ac3bb9fe3b40" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchaseOfTreasuryShares_01a1878f-445d-7c9d-92d2-30c9fbddcbec" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PurchaseOfTreasuryShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_PurchaseOfTreasuryShares_01a1878f-445d-7c9d-92d2-30c9fbddcbec" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SharePremiumDistributionEquity_01a1878f-445d-7aaa-9637-b14eaa5bd955" xlink:href="jbsay-20260930.xsd#jbsay_SharePremiumDistributionEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_SharePremiumDistributionEquity_01a1878f-445d-7aaa-9637-b14eaa5bd955" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncreaseDecreaseThroughListingCostsEquity_01a1878f-445d-7308-ad96-c31b153c7e8d" xlink:href="jbsay-20260930.xsd#jbsay_IncreaseDecreaseThroughListingCostsEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_IncreaseDecreaseThroughListingCostsEquity_01a1878f-445d-7308-ad96-c31b153c7e8d" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_StockOptionPlan_01a18794-98b1-741f-820a-2cd6f83caf05" xlink:href="jbsay-20260930.xsd#jbsay_StockOptionPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_StockOptionPlan_01a18794-98b1-741f-820a-2cd6f83caf05" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TransferOfTreasuryShares_01a18794-98b1-7ea6-a57d-1300d3f58e3c" xlink:href="jbsay-20260930.xsd#jbsay_TransferOfTreasuryShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_TransferOfTreasuryShares_01a18794-98b1-7ea6-a57d-1300d3f58e3c" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions_01a1878f-445d-7867-9c8a-9333bebc992d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions_01a1878f-445d-7867-9c8a-9333bebc992d" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncreaseDecreaseThroughRealizationOfOtherReserves_01a1878f-445d-7559-9f53-b888061d3462" xlink:href="jbsay-20260930.xsd#jbsay_IncreaseDecreaseThroughRealizationOfOtherReserves"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_IncreaseDecreaseThroughRealizationOfOtherReserves_01a1878f-445d-7559-9f53-b888061d3462" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncreaseDecreaseThroughDistributionOfProposedInterimDividends_01a1878f-445d-79fd-aec1-f4f20ced952f" xlink:href="jbsay-20260930.xsd#jbsay_IncreaseDecreaseThroughDistributionOfProposedInterimDividends"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_IncreaseDecreaseThroughDistributionOfProposedInterimDividends_01a1878f-445d-79fd-aec1-f4f20ced952f" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DividendsDeclared_01a1878f-445d-70c3-a2e2-3c658d31f4a8" xlink:href="jbsay-20260930.xsd#jbsay_DividendsDeclared"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_DividendsDeclared_01a1878f-445d-70c3-a2e2-3c658d31f4a8" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainRecognisedInBargainPurchaseTransaction_01a1878f-445d-7c8f-b016-16e40c00e844" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainRecognisedInBargainPurchaseTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_GainRecognisedInBargainPurchaseTransaction_01a1878f-445d-7c8f-b016-16e40c00e844" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsPaid_01a1878f-445d-7f9e-aee9-d04995212ea4" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DividendsPaid"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_DividendsPaid_01a1878f-445d-7f9e-aee9-d04995212ea4" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AcquisitionOfTreasuryShares_01a189d1-00a6-7e78-81ab-7f4442e96697" xlink:href="jbsay-20260930.xsd#jbsay_AcquisitionOfTreasuryShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_jbsay_AcquisitionOfTreasuryShares_01a189d1-00a6-7e78-81ab-7f4442e96697" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsRecognisedAsDistributionsToNoncontrollingInterests_01a18794-f2c5-72fd-94ff-4af42403372e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DividendsRecognisedAsDistributionsToNoncontrollingInterests"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_DividendsRecognisedAsDistributionsToNoncontrollingInterests_01a18794-f2c5-72fd-94ff-4af42403372e" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesEquity_01a1878f-445d-781e-bb5f-72cafc6a753a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_StatementOfChangesInEquityLineItems_01a1878f-445d-7344-adf0-152e3b554cb6" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesEquity_01a1878f-445d-781e-bb5f-72cafc6a753a" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Equity_01a1878f-445d-7ff4-9e8e-c38789101b12" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Equity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/CondensedStatementsofCashFlows" xlink:type="simple" xlink:href="jbsay-20260930.xsd#CondensedStatementsofCashFlows"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/CondensedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:href="jbsay-20260930.xsd#jbsay_Statement1LineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_Statement1Table_01a188ee-d062-70aa-a607-47c4806fd7cd" xlink:href="jbsay-20260930.xsd#jbsay_Statement1Table"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_jbsay_Statement1Table_01a188ee-d062-70aa-a607-47c4806fd7cd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsAxis_01a188ee-d062-7994-99b0-45d412983b3c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_Statement1Table_01a188ee-d062-70aa-a607-47c4806fd7cd" xlink:to="loc_ifrs-full_BusinessCombinationsAxis_01a188ee-d062-7994-99b0-45d412983b3c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_01a188ee-d062-7994-99b0-45d412983b3c_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_01a188ee-d062-7994-99b0-45d412983b3c" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_01a188ee-d062-7994-99b0-45d412983b3c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_01a188ef-28bc-7fda-9d92-8f16493fcff8" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_01a188ee-d062-7994-99b0-45d412983b3c" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_01a188ef-28bc-7fda-9d92-8f16493fcff8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AcquisitionOfNonControllingInterestsInTheSubsidiaryPPCMember_01a188ef-28bc-7732-b77f-9ca77d52ee0a" xlink:href="jbsay-20260930.xsd#jbsay_AcquisitionOfNonControllingInterestsInTheSubsidiaryPPCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a188ef-28bc-7fda-9d92-8f16493fcff8" xlink:to="loc_jbsay_AcquisitionOfNonControllingInterestsInTheSubsidiaryPPCMember_01a188ef-28bc-7732-b77f-9ca77d52ee0a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AcquisitionOfNonControllingInterestsInAndrewMeatsMember_01a188ef-5f0a-72b4-89e8-48143fbd0c37" xlink:href="jbsay-20260930.xsd#jbsay_AcquisitionOfNonControllingInterestsInAndrewMeatsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a188ef-28bc-7fda-9d92-8f16493fcff8" xlink:to="loc_jbsay_AcquisitionOfNonControllingInterestsInAndrewMeatsMember_01a188ef-5f0a-72b4-89e8-48143fbd0c37" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_01a0582c-fb13-7ea1-8b91-c203999d6fb4" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:to="loc_ifrs-full_ProfitLoss_01a0582c-fb13-7ea1-8b91-c203999d6fb4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForReconcileProfitLossAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProfitLoss_01a0582c-fb13-7ea1-8b91-c203999d6fb4" xlink:to="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForDepreciationAndAmortisationExpense_01a0582c-fb13-731b-a4f9-244ca69f436f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDepreciationAndAmortisationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForDepreciationAndAmortisationExpense_01a0582c-fb13-731b-a4f9-244ca69f436f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustmentsForExpectedCreditLosses_01a0582c-fb13-7e64-91bd-16f530db1464" xlink:href="jbsay-20260930.xsd#jbsay_AdjustmentsForExpectedCreditLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_jbsay_AdjustmentsForExpectedCreditLosses_01a0582c-fb13-7e64-91bd-16f530db1464" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustmentsForShareOfProfitLossOfEquityAccountedInvestees_01a0582c-fb13-7293-987a-184207ce9ade" xlink:href="jbsay-20260930.xsd#jbsay_AdjustmentsForShareOfProfitLossOfEquityAccountedInvestees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_jbsay_AdjustmentsForShareOfProfitLossOfEquityAccountedInvestees_01a0582c-fb13-7293-987a-184207ce9ade" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForLossesGainsOnDisposalOfNoncurrentAssets_01a0582c-fb13-763c-8c5d-71d5b15b2a26" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForLossesGainsOnDisposalOfNoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForLossesGainsOnDisposalOfNoncurrentAssets_01a0582c-fb13-763c-8c5d-71d5b15b2a26" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForIncomeTaxExpense_01a0582c-fb13-749f-bc0f-614eae3bae25" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForIncomeTaxExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForIncomeTaxExpense_01a0582c-fb13-749f-bc0f-614eae3bae25" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForFinanceCosts_01a0582c-fb13-7671-8851-6d77baae45d4" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForFinanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForFinanceCosts_01a0582c-fb13-7671-8851-6d77baae45d4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForSharebasedPayments_01a0582c-fb13-737c-b9b6-bfc2f1de0b64" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForSharebasedPayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForSharebasedPayments_01a0582c-fb13-737c-b9b6-bfc2f1de0b64" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForProvisions_01a0582c-fb13-7390-918d-6fafbacdc6c0" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForProvisions_01a0582c-fb13-7390-918d-6fafbacdc6c0" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLoss_01a0582c-fb13-7880-a35c-402f6bfd1d32" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_AdjustmentsForImpairmentLossReversalOfImpairmentLossRecognisedInProfitOrLoss_01a0582c-fb13-7880-a35c-402f6bfd1d32" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustmentsForNetRealizableValueInventoryAdjustments_01a0582c-fb13-70b3-bbb9-a56941bce3f6" xlink:href="jbsay-20260930.xsd#jbsay_AdjustmentsForNetRealizableValueInventoryAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_jbsay_AdjustmentsForNetRealizableValueInventoryAdjustments_01a0582c-fb13-70b3-bbb9-a56941bce3f6" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustmentsForDepartmentOfJusticeAndAntitrustAgreements_01a0582c-fb13-7dd0-a064-ff10c6b203eb" xlink:href="jbsay-20260930.xsd#jbsay_AdjustmentsForDepartmentOfJusticeAndAntitrustAgreements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_jbsay_AdjustmentsForDepartmentOfJusticeAndAntitrustAgreements_01a0582c-fb13-7dd0-a064-ff10c6b203eb" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnFairValueAdjustmentBiologicalAssets_01a0582c-fb13-7333-86ce-094f8fde14f3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnFairValueAdjustmentBiologicalAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_ifrs-full_GainsLossesOnFairValueAdjustmentBiologicalAssets_01a0582c-fb13-7333-86ce-094f8fde14f3" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProvisionForAvianInfluenzaRelatedCosts_01a0582c-fb13-7e74-a3e3-3918a78a446f" xlink:href="jbsay-20260930.xsd#jbsay_ProvisionForAvianInfluenzaRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AdjustmentsForReconcileProfitLossAbstract_01a0582c-fb13-726e-a4cc-e87e036d8862" xlink:to="loc_jbsay_ProvisionForAvianInfluenzaRelatedCosts_01a0582c-fb13-7e74-a3e3-3918a78a446f" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CashFlowsFromUsedInOperationsExcludingChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities_01a0582c-fb13-7727-9dbe-c8b4ab548fc5" xlink:href="jbsay-20260930.xsd#jbsay_CashFlowsFromUsedInOperationsExcludingChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:to="loc_jbsay_CashFlowsFromUsedInOperationsExcludingChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities_01a0582c-fb13-7727-9dbe-c8b4ab548fc5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:href="jbsay-20260930.xsd#jbsay_ChangesInAssetsAndLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_CashFlowsFromUsedInOperationsExcludingChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities_01a0582c-fb13-7727-9dbe-c8b4ab548fc5" xlink:to="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInTradeAccountReceivable_01a0582c-fb13-74ad-955f-159a30d1e996" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDecreaseIncreaseInTradeAccountReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInTradeAccountReceivable_01a0582c-fb13-74ad-955f-159a30d1e996" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInInventories_01a0582c-fb13-73ea-85f1-f34fc6b4c352" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDecreaseIncreaseInInventories"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInInventories_01a0582c-fb13-73ea-85f1-f34fc6b4c352" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustmentsForDecreaseIncreaseInRecoverablesTaxes_01a0582c-fb13-777b-b2b7-7f90737914b1" xlink:href="jbsay-20260930.xsd#jbsay_AdjustmentsForDecreaseIncreaseInRecoverablesTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_jbsay_AdjustmentsForDecreaseIncreaseInRecoverablesTaxes_01a0582c-fb13-777b-b2b7-7f90737914b1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInOtherCurrentAssets_01a0582c-fb13-7a47-a5f1-d02fd2a6c0d2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDecreaseIncreaseInOtherCurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInOtherCurrentAssets_01a0582c-fb13-7a47-a5f1-d02fd2a6c0d2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInBiologicalAssets_01a0582c-fb13-75ce-9ca2-9cee418dce15" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForDecreaseIncreaseInBiologicalAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_AdjustmentsForDecreaseIncreaseInBiologicalAssets_01a0582c-fb13-75ce-9ca2-9cee418dce15" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForIncreaseDecreaseInTradeAccountPayable_01a0582c-fb13-7d69-a398-095402a9c3e8" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForIncreaseDecreaseInTradeAccountPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_AdjustmentsForIncreaseDecreaseInTradeAccountPayable_01a0582c-fb13-7d69-a398-095402a9c3e8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncreaseDecreaseInTaxesPaidInInstallments_01a0582c-fb13-7cf5-92d5-4c2d183e2c8b" xlink:href="jbsay-20260930.xsd#jbsay_IncreaseDecreaseInTaxesPaidInInstallments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_jbsay_IncreaseDecreaseInTaxesPaidInInstallments_01a0582c-fb13-7cf5-92d5-4c2d183e2c8b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdjustmentsForIncreaseDecreaseInOtherLiabilities_01a0582c-fb13-75fc-9b9d-b59fa54e9155" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdjustmentsForIncreaseDecreaseInOtherLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_AdjustmentsForIncreaseDecreaseInOtherLiabilities_01a0582c-fb13-75fc-9b9d-b59fa54e9155" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustmentsForIncreaseDecreaseInDepartmentOfJusticeAndAntitrustAgreementsPayment_01a0a535-af56-7bb2-a92a-d9f946ec9e6f" xlink:href="jbsay-20260930.xsd#jbsay_AdjustmentsForIncreaseDecreaseInDepartmentOfJusticeAndAntitrustAgreementsPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_jbsay_AdjustmentsForIncreaseDecreaseInDepartmentOfJusticeAndAntitrustAgreementsPayment_01a0a535-af56-7bb2-a92a-d9f946ec9e6f" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncomeTaxesPaidRefundClassifiedAsOperatingActivities_01a0582c-fb13-7c3a-b8f1-e5499882b6bf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncomeTaxesPaidRefundClassifiedAsOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_ifrs-full_IncomeTaxesPaidRefundClassifiedAsOperatingActivities_01a0582c-fb13-7c3a-b8f1-e5499882b6bf" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities_01a0582c-fb13-7a6d-b51d-c6321214adb8" xlink:href="jbsay-20260930.xsd#jbsay_ChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ChangesInAssetsAndLiabilitiesAbstract_01a0582c-fb13-7bfa-b8db-21e938695f4c" xlink:to="loc_jbsay_ChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities_01a0582c-fb13-7a6d-b51d-c6321214adb8" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInOperations_01a0582c-fb13-79a6-92cd-c7ae23354476" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_CashFlowsFromUsedInOperationsExcludingChangesInOperatingAssetsAndLiabilitiesClassifiedAsOperatingActivities_01a0582c-fb13-7727-9dbe-c8b4ab548fc5" xlink:to="loc_ifrs-full_CashFlowsFromUsedInOperations_01a0582c-fb13-79a6-92cd-c7ae23354476" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InterestPaidClassifiedAsOperatingActivities_01a0582c-fb13-768a-a785-3f6f47d44805" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InterestPaidClassifiedAsOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:to="loc_ifrs-full_InterestPaidClassifiedAsOperatingActivities_01a0582c-fb13-768a-a785-3f6f47d44805" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InterestReceivedClassifiedAsOperatingActivities_01a0582c-fb13-79ad-bbcc-917bad4eebbd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InterestReceivedClassifiedAsOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:to="loc_ifrs-full_InterestReceivedClassifiedAsOperatingActivities_01a0582c-fb13-79ad-bbcc-917bad4eebbd" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInOperatingActivities_01a0582c-fb13-7c30-bb8a-e1e8ad70dbdb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInOperatingActivitiesAbstract_01a0582c-fb13-7550-9617-89367a49c6b8" xlink:to="loc_ifrs-full_CashFlowsFromUsedInOperatingActivities_01a0582c-fb13-7c30-bb8a-e1e8ad70dbdb" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchaseOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities_01a0582c-fb13-78b5-a759-076c76224b55" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PurchaseOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_ifrs-full_PurchaseOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities_01a0582c-fb13-78b5-a759-076c76224b55" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsReceivedClassifiedAsInvestingActivities_01a0582c-fb13-7120-9950-fd881d8da329" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DividendsReceivedClassifiedAsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_ifrs-full_DividendsReceivedClassifiedAsInvestingActivities_01a0582c-fb13-7120-9950-fd881d8da329" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PurchaseOfOtherLongtermAssetsClassifiedAsInvestingActivities_01a0582c-fb13-7ace-a969-30e5df720ef1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PurchaseOfOtherLongtermAssetsClassifiedAsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_ifrs-full_PurchaseOfOtherLongtermAssetsClassifiedAsInvestingActivities_01a0582c-fb13-7ace-a969-30e5df720ef1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherInflowsOutflowsOfCashClassifiedAsInvestingActivities_01a0582c-fb13-7a4e-b494-857c12e8cd09" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherInflowsOutflowsOfCashClassifiedAsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_ifrs-full_OtherInflowsOutflowsOfCashClassifiedAsInvestingActivities_01a0582c-fb13-7a4e-b494-857c12e8cd09" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsClassifiedAsInvestingActivities_01a0582c-fb13-7971-92f7-7a8e74ed2823" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsClassifiedAsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_jbsay_RelatedPartyTransactionsClassifiedAsInvestingActivities_01a0582c-fb13-7971-92f7-7a8e74ed2823" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProceedsFromSalesOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities_01a0582c-fb13-7548-8b31-d8b3ebe4e41f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromSalesOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_ifrs-full_ProceedsFromSalesOfPropertyPlantAndEquipmentClassifiedAsInvestingActivities_01a0582c-fb13-7548-8b31-d8b3ebe4e41f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AcquisitionOfInvestmentsFunds_01a0f125-8cf8-7a2b-99d6-b669c4973103" xlink:href="jbsay-20260930.xsd#jbsay_AcquisitionOfInvestmentsFunds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_jbsay_AcquisitionOfInvestmentsFunds_01a0f125-8cf8-7a2b-99d6-b669c4973103" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInInvestingActivities_01a0f126-0011-7f13-a38b-655b4169f3dd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInInvestingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInInvestingActivitiesAbstract_01a0582c-fb13-7896-859e-5c291cb5256d" xlink:to="loc_ifrs-full_CashFlowsFromUsedInInvestingActivities_01a0f126-0011-7f13-a38b-655b4169f3dd" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProceedsFromBorrowingsClassifiedAsFinancingActivities_01a0582c-fb13-7695-8aa9-820d93584668" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromBorrowingsClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_ifrs-full_ProceedsFromBorrowingsClassifiedAsFinancingActivities_01a0582c-fb13-7695-8aa9-820d93584668" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PaymentsOfLoansAndFinancingClassifiedAsFinancingActivities_01a0582c-fb13-7749-9183-9956a146b18e" xlink:href="jbsay-20260930.xsd#jbsay_PaymentsOfLoansAndFinancingClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_jbsay_PaymentsOfLoansAndFinancingClassifiedAsFinancingActivities_01a0582c-fb13-7749-9183-9956a146b18e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DerivativeInstrumentsReceivedSettledClassifiedAsFinancingActivities_01a0f12b-5305-7347-ade8-29da965009be" xlink:href="jbsay-20260930.xsd#jbsay_DerivativeInstrumentsReceivedSettledClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_jbsay_DerivativeInstrumentsReceivedSettledClassifiedAsFinancingActivities_01a0f12b-5305-7347-ade8-29da965009be" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MarginCashClassifiedAsFinancingActivities_01a0582c-fb13-78fd-9de4-0aaa0339b2e3" xlink:href="jbsay-20260930.xsd#jbsay_MarginCashClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_jbsay_MarginCashClassifiedAsFinancingActivities_01a0582c-fb13-78fd-9de4-0aaa0339b2e3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsPaidClassifiedAsFinancingActivities_01a1876f-24b2-70a8-a0f8-b29dab1ee9ff" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DividendsPaidClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_ifrs-full_DividendsPaidClassifiedAsFinancingActivities_01a1876f-24b2-70a8-a0f8-b29dab1ee9ff" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendsPaidToNoncontrollingInterestsClassifiedAsFinancingActivities_01a0582c-fb13-7645-bbfe-4a16518415b6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DividendsPaidToNoncontrollingInterestsClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_ifrs-full_DividendsPaidToNoncontrollingInterestsClassifiedAsFinancingActivities_01a0582c-fb13-7645-bbfe-4a16518415b6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ShareBuybackClassifiedAsFinancingActivities_01a15837-0f79-7791-b623-8b43a03d85f1" xlink:href="jbsay-20260930.xsd#jbsay_ShareBuybackClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_jbsay_ShareBuybackClassifiedAsFinancingActivities_01a15837-0f79-7791-b623-8b43a03d85f1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PaymentsOfLeaseLiabilitiesClassifiedAsFinancingActivities_01a0582c-fb13-7207-bb80-9184475db4ef" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PaymentsOfLeaseLiabilitiesClassifiedAsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_ifrs-full_PaymentsOfLeaseLiabilitiesClassifiedAsFinancingActivities_01a0582c-fb13-7207-bb80-9184475db4ef" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PaymentsFromChangesInOwnershipInterestsInSubsidiaries_01a0f120-79e9-77a0-8499-f069f4daa7e2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PaymentsFromChangesInOwnershipInterestsInSubsidiaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_ifrs-full_PaymentsFromChangesInOwnershipInterestsInSubsidiaries_01a0f120-79e9-77a0-8499-f069f4daa7e2" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OtherFinancialAssetsFinancingActivities_01a0f12c-22a6-7e15-bafc-12146bc9562f" xlink:href="jbsay-20260930.xsd#jbsay_OtherFinancialAssetsFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_jbsay_OtherFinancialAssetsFinancingActivities_01a0f12c-22a6-7e15-bafc-12146bc9562f" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashFlowsFromUsedInFinancingActivities_01a0582c-fb13-7756-a67f-87d1f46fd3d7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashFlowsFromUsedInFinancingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CashFlowsFromUsedInFinancingActivitiesAbstract_01a0582c-fb13-7745-b610-4c5258d7f13d" xlink:to="loc_ifrs-full_CashFlowsFromUsedInFinancingActivities_01a0582c-fb13-7756-a67f-87d1f46fd3d7" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EffectOfExchangeRateChangesOnCashAndCashEquivalents_01a0582c-fb13-7615-92c9-0b45a0347c02" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EffectOfExchangeRateChangesOnCashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_ifrs-full_EffectOfExchangeRateChangesOnCashAndCashEquivalents_01a0582c-fb13-7615-92c9-0b45a0347c02" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseInCashAndCashEquivalents_01a13090-40a8-783d-b09e-9c86ae2f7ffe" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseInCashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_ifrs-full_IncreaseDecreaseInCashAndCashEquivalents_01a13090-40a8-783d-b09e-9c86ae2f7ffe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashAndCashEquivalents_01a13091-718c-7b18-93ac-86e267fbb168" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_ifrs-full_CashAndCashEquivalents_01a13091-718c-7b18-93ac-86e267fbb168" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashAndCashEquivalents_01a13092-62b8-7d23-a5e0-c66ffed861a2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashAndCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CashFlowNonCashTransactionsAbstract_01a0f139-8dfe-7413-a4e4-debfa093926a" xlink:href="jbsay-20260930.xsd#jbsay_CashFlowNonCashTransactionsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Statement1LineItems_01a188ee-d062-70f4-8f15-aa19ba757c18" xlink:to="loc_jbsay_CashFlowNonCashTransactionsAbstract_01a0f139-8dfe-7413-a4e4-debfa093926a" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NoncashAdditionsToRightOfUseAssetsAndLeaseLiabilities_01a0582c-fb13-7776-a43e-7765e527d37a" xlink:href="jbsay-20260930.xsd#jbsay_NoncashAdditionsToRightOfUseAssetsAndLeaseLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_CashFlowNonCashTransactionsAbstract_01a0f139-8dfe-7413-a4e4-debfa093926a" xlink:to="loc_jbsay_NoncashAdditionsToRightOfUseAssetsAndLeaseLiabilities_01a0582c-fb13-7776-a43e-7765e527d37a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CapitalizedInterest_01a0582c-fb13-7bb4-8b4c-f9452e001436" xlink:href="jbsay-20260930.xsd#jbsay_CapitalizedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_CashFlowNonCashTransactionsAbstract_01a0f139-8dfe-7413-a4e4-debfa093926a" xlink:to="loc_jbsay_CapitalizedInterest_01a0582c-fb13-7bb4-8b4c-f9452e001436" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_GainRecognisedInBargainPurchaseTransactionAdjusted_01a0582c-fb13-7ee7-9fde-0eba330bf7e7" xlink:href="jbsay-20260930.xsd#jbsay_GainRecognisedInBargainPurchaseTransactionAdjusted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_CashFlowNonCashTransactionsAbstract_01a0f139-8dfe-7413-a4e4-debfa093926a" xlink:to="loc_jbsay_GainRecognisedInBargainPurchaseTransactionAdjusted_01a0582c-fb13-7ee7-9fde-0eba330bf7e7" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/BackgroundInformationDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#BackgroundInformationDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/BackgroundInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:href="jbsay-20260930.xsd#jbsay_BackgroundInformationDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:href="jbsay-20260930.xsd#jbsay_BackgroundInformationDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_JointVenturesAxis_01a09e90-dec3-7664-913e-312ebcb114e2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_JointVenturesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_ifrs-full_JointVenturesAxis_01a09e90-dec3-7664-913e-312ebcb114e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_JointVenturesDomain_01a09e90-dec3-7664-913e-312ebcb114e2_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_JointVenturesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_JointVenturesAxis_01a09e90-dec3-7664-913e-312ebcb114e2" xlink:to="loc_ifrs-full_JointVenturesDomain_01a09e90-dec3-7664-913e-312ebcb114e2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_JointVenturesDomain_01a09e90-dec3-7d16-a174-75e7e5d59e48" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_JointVenturesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_JointVenturesAxis_01a09e90-dec3-7664-913e-312ebcb114e2" xlink:to="loc_ifrs-full_JointVenturesDomain_01a09e90-dec3-7d16-a174-75e7e5d59e48" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DanantaraInvestmentManagementMember_01a09e90-dec3-746e-90c0-139fc286a460" xlink:href="jbsay-20260930.xsd#jbsay_DanantaraInvestmentManagementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_JointVenturesDomain_01a09e90-dec3-7d16-a174-75e7e5d59e48" xlink:to="loc_jbsay_DanantaraInvestmentManagementMember_01a09e90-dec3-746e-90c0-139fc286a460" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalAxis_01a1587f-f487-7f1f-845c-f35414d3241b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfShareCapitalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_ifrs-full_ClassesOfShareCapitalAxis_01a1587f-f487-7f1f-845c-f35414d3241b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_01a1587f-f487-7f1f-845c-f35414d3241b_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_01a1587f-f487-7f1f-845c-f35414d3241b" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_01a1587f-f487-7f1f-845c-f35414d3241b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfShareCapitalDomain_01a1587f-f487-76c4-b6f2-5ce3f85eced2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfShareCapitalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfShareCapitalAxis_01a1587f-f487-7f1f-845c-f35414d3241b" xlink:to="loc_ifrs-full_ClassesOfShareCapitalDomain_01a1587f-f487-76c4-b6f2-5ce3f85eced2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ClassACommonStockMember_01a1587f-f487-7eab-8539-03308c013b5c" xlink:href="jbsay-20260930.xsd#jbsay_ClassACommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfShareCapitalDomain_01a1587f-f487-76c4-b6f2-5ce3f85eced2" xlink:to="loc_jbsay_ClassACommonStockMember_01a1587f-f487-7eab-8539-03308c013b5c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_01a1587d-9e18-7bd1-9ab2-14cc3d6e8bcc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_01a1587d-9e18-7bd1-9ab2-14cc3d6e8bcc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_01a1587d-9e18-7bd1-9ab2-14cc3d6e8bcc_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_01a1587d-9e18-7bd1-9ab2-14cc3d6e8bcc" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_01a1587d-9e18-7bd1-9ab2-14cc3d6e8bcc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_01a1587d-9e18-790d-8315-e24460843457" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SignificantInvestmentsInSubsidiariesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesAxis_01a1587d-9e18-7bd1-9ab2-14cc3d6e8bcc" xlink:to="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_01a1587d-9e18-790d-8315-e24460843457" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_JBSS.A.Member_01a1587d-9e18-7cb7-8625-89dca7b6152c" xlink:href="jbsay-20260930.xsd#jbsay_JBSS.A.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_01a1587d-9e18-790d-8315-e24460843457" xlink:to="loc_jbsay_JBSS.A.Member_01a1587d-9e18-7cb7-8625-89dca7b6152c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_VIVAHoldingMember_01a1587d-9e18-72bb-8fbb-d5bdca1838e1" xlink:href="jbsay-20260930.xsd#jbsay_VIVAHoldingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInSubsidiariesDomain_01a1587d-9e18-790d-8315-e24460843457" xlink:to="loc_jbsay_VIVAHoldingMember_01a1587d-9e18-72bb-8fbb-d5bdca1838e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsAxis_01a187cb-0d9b-7e18-8efb-1b99cbea5186" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_ifrs-full_BusinessCombinationsAxis_01a187cb-0d9b-7e18-8efb-1b99cbea5186" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7e18-8efb-1b99cbea5186_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_01a187cb-0d9b-7e18-8efb-1b99cbea5186" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7e18-8efb-1b99cbea5186_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7304-897f-395809699ae1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BusinessCombinationsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_BusinessCombinationsAxis_01a187cb-0d9b-7e18-8efb-1b99cbea5186" xlink:to="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7304-897f-395809699ae1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WalkersDeliSausageCompanyMember_01a187cb-63cd-7e48-917c-f10d95042853" xlink:href="jbsay-20260930.xsd#jbsay_WalkersDeliSausageCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7304-897f-395809699ae1" xlink:to="loc_jbsay_WalkersDeliSausageCompanyMember_01a187cb-63cd-7e48-917c-f10d95042853" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FloraProdutosDeHigieneELimpezaSAMember_01a1c0cc-8954-7187-998f-cdc47d2d2c90" xlink:href="jbsay-20260930.xsd#jbsay_FloraProdutosDeHigieneELimpezaSAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7304-897f-395809699ae1" xlink:to="loc_jbsay_FloraProdutosDeHigieneELimpezaSAMember_01a1c0cc-8954-7187-998f-cdc47d2d2c90" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CanalRuralMember_01a1c0cc-e829-7558-9af4-1fc829ae105f" xlink:href="jbsay-20260930.xsd#jbsay_CanalRuralMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BusinessCombinationsDomain_01a187cb-0d9b-7304-897f-395809699ae1" xlink:to="loc_jbsay_CanalRuralMember_01a1c0cc-e829-7558-9af4-1fc829ae105f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BorrowingsByNameAxis_01a187d6-5a33-7a8b-bf91-0bb2b963141f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BorrowingsByNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_ifrs-full_BorrowingsByNameAxis_01a187d6-5a33-7a8b-bf91-0bb2b963141f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BorrowingsByNameDomain_01a187d6-5a33-7a8b-bf91-0bb2b963141f_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BorrowingsByNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_BorrowingsByNameAxis_01a187d6-5a33-7a8b-bf91-0bb2b963141f" xlink:to="loc_ifrs-full_BorrowingsByNameDomain_01a187d6-5a33-7a8b-bf91-0bb2b963141f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BorrowingsByNameDomain_01a187d6-5a33-7d27-89af-2270aed950ae" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BorrowingsByNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_BorrowingsByNameAxis_01a187d6-5a33-7a8b-bf91-0bb2b963141f" xlink:to="loc_ifrs-full_BorrowingsByNameDomain_01a187d6-5a33-7d27-89af-2270aed950ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SeniorNotes4.750Due2034Member_01a187d6-5a33-766f-a30f-1ed1544b12ef" xlink:href="jbsay-20260930.xsd#jbsay_SeniorNotes4.750Due2034Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BorrowingsByNameDomain_01a187d6-5a33-7d27-89af-2270aed950ae" xlink:to="loc_jbsay_SeniorNotes4.750Due2034Member_01a187d6-5a33-766f-a30f-1ed1544b12ef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SeniorNotesDue2037And2057Member_01a187dd-5dfe-79e4-9a03-cfd5bebb227a" xlink:href="jbsay-20260930.xsd#jbsay_SeniorNotesDue2037And2057Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_BorrowingsByNameDomain_01a187d6-5a33-7d27-89af-2270aed950ae" xlink:to="loc_jbsay_SeniorNotesDue2037And2057Member_01a187dd-5dfe-79e4-9a03-cfd5bebb227a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_01a19b86-b2bd-7631-855f-cc701cd6fb56" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_01a19b86-b2bd-7631-855f-cc701cd6fb56" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_01a19b86-b2bd-7631-855f-cc701cd6fb56_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_01a19b86-b2bd-7631-855f-cc701cd6fb56" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_01a19b86-b2bd-7631-855f-cc701cd6fb56_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_01a19b86-b2bd-7ffd-969a-93ec998749bf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodAxis_01a19b86-b2bd-7631-855f-cc701cd6fb56" xlink:to="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_01a19b86-b2bd-7ffd-969a-93ec998749bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonAdjustingEventsAfterReportingPeriodMember_01a19b86-b2bd-75f7-b4ad-e4137b59b1cc" xlink:href="jbsay-20260930.xsd#jbsay_NonAdjustingEventsAfterReportingPeriodMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NonadjustingEventsAfterReportingPeriodDomain_01a19b86-b2bd-7ffd-969a-93ec998749bf" xlink:to="loc_jbsay_NonAdjustingEventsAfterReportingPeriodMember_01a19b86-b2bd-75f7-b4ad-e4137b59b1cc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_01a1b1e7-504a-78fd-b1a8-6052b3db7e66" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_srt_TitleOfIndividualAxis_01a1b1e7-504a-78fd-b1a8-6052b3db7e66" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_01a1b1e7-504a-78fd-b1a8-6052b3db7e66_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_01a1b1e7-504a-78fd-b1a8-6052b3db7e66" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_01a1b1e7-504a-78fd-b1a8-6052b3db7e66_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_01a1b1e7-504a-7dad-a187-ce3643addad7" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_01a1b1e7-504a-78fd-b1a8-6052b3db7e66" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_01a1b1e7-504a-7dad-a187-ce3643addad7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ChiefExecutiveOfficerMember_01a1b1e8-017b-7dd6-bd7d-250595398fb9" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ChiefExecutiveOfficerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_01a1b1e7-504a-7dad-a187-ce3643addad7" xlink:to="loc_srt_ChiefExecutiveOfficerMember_01a1b1e8-017b-7dd6-bd7d-250595398fb9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_01a1b1e8-6306-717c-8c3f-e030d316cd6d" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_BackgroundInformationDetailsTable_01a0582c-fb13-776c-a1c2-2c6f3d16da26" xlink:to="loc_srt_CounterpartyNameAxis_01a1b1e8-6306-717c-8c3f-e030d316cd6d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_01a1b1e8-6306-717c-8c3f-e030d316cd6d_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_01a1b1e8-6306-717c-8c3f-e030d316cd6d" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_01a1b1e8-6306-717c-8c3f-e030d316cd6d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_01a1b1e8-6306-70c9-85de-9f345ae7fedf" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_01a1b1e8-6306-717c-8c3f-e030d316cd6d" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_01a1b1e8-6306-70c9-85de-9f345ae7fedf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WesleyBatistaFilhoMember_01a1b1e8-9587-715c-ac24-06a2a359e8c3" xlink:href="jbsay-20260930.xsd#jbsay_WesleyBatistaFilhoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_01a1b1e8-6306-70c9-85de-9f345ae7fedf" xlink:to="loc_jbsay_WesleyBatistaFilhoMember_01a1b1e8-9587-715c-ac24-06a2a359e8c3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_GilbertoTomazoniMember_01a1b1e8-e409-7d9f-9bdc-ae77b788caee" xlink:href="jbsay-20260930.xsd#jbsay_GilbertoTomazoniMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_01a1b1e8-6306-70c9-85de-9f345ae7fedf" xlink:to="loc_jbsay_GilbertoTomazoniMember_01a1b1e8-e409-7d9f-9bdc-ae77b788caee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DebtInstrumentsIssuedNumberOfBonds_01a09e90-dec3-7cbe-a2e2-758d5f5feeed" xlink:href="jbsay-20260930.xsd#jbsay_DebtInstrumentsIssuedNumberOfBonds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_DebtInstrumentsIssuedNumberOfBonds_01a09e90-dec3-7cbe-a2e2-758d5f5feeed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DebtInstrumentUnitFaceValue_01a09e90-dec3-72a0-8b23-4471e8b80b14" xlink:href="jbsay-20260930.xsd#jbsay_DebtInstrumentUnitFaceValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_DebtInstrumentUnitFaceValue_01a09e90-dec3-72a0-8b23-4471e8b80b14" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DebtInstrumentFaceValue_01a09e90-dec3-7427-8c88-4a0a3f94aa6d" xlink:href="jbsay-20260930.xsd#jbsay_DebtInstrumentFaceValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_DebtInstrumentFaceValue_01a09e90-dec3-7427-8c88-4a0a3f94aa6d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProportionOfOwnershipInterestInJointVenture_01a09e90-dec3-73b3-bc4e-6dfb5913917a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProportionOfOwnershipInterestInJointVenture"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_ProportionOfOwnershipInterestInJointVenture_01a09e90-dec3-73b3-bc4e-6dfb5913917a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CommitmentToInvestInJointVenture_01a09e90-dec3-7b2f-922d-ae5d897358c7" xlink:href="jbsay-20260930.xsd#jbsay_CommitmentToInvestInJointVenture"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_CommitmentToInvestInJointVenture_01a09e90-dec3-7b2f-922d-ae5d897358c7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InvestmentsInJointVentures_01a09e90-dec3-75a2-be9e-00d8ebfaa4de" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InvestmentsInJointVentures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_InvestmentsInJointVentures_01a09e90-dec3-75a2-be9e-00d8ebfaa4de" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TunureAtCompany_01a1b1e6-75e7-740b-a515-3bf244531ccf" xlink:href="jbsay-20260930.xsd#jbsay_TunureAtCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_TunureAtCompany_01a1b1e6-75e7-740b-a515-3bf244531ccf" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_YearsServedAtCompany_01a1b1e6-de00-7e0b-973c-4e30bdad19f9" xlink:href="jbsay-20260930.xsd#jbsay_YearsServedAtCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_YearsServedAtCompany_01a1b1e6-de00-7e0b-973c-4e30bdad19f9" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashTransferred_01a187ca-b81e-76d3-8fef-1240fe546a42" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_CashTransferred_01a187ca-b81e-76d3-8fef-1240fe546a42" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BusinessCombinationNonAffiliatedFixedExchangeRatio_01a1587d-9e18-71b7-9016-b23454737544" xlink:href="jbsay-20260930.xsd#jbsay_BusinessCombinationNonAffiliatedFixedExchangeRatio"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_BusinessCombinationNonAffiliatedFixedExchangeRatio_01a1587d-9e18-71b7-9016-b23454737544" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProportionOfOwnershipInterestsOfSubsidiaries_01a1587d-9e18-772b-97f1-84956c8855ba" xlink:href="jbsay-20260930.xsd#jbsay_ProportionOfOwnershipInterestsOfSubsidiaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_jbsay_ProportionOfOwnershipInterestsOfSubsidiaries_01a1587d-9e18-772b-97f1-84956c8855ba" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Borrowings_01a187d7-540c-7509-8917-f5e30c3a2f23" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Borrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_Borrowings_01a187d7-540c-7509-8917-f5e30c3a2f23" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BorrowingsInterestRate_01a187d7-540c-77e9-9427-74c4e43b9404" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BorrowingsInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_BorrowingsInterestRate_01a187d7-540c-77e9-9427-74c4e43b9404" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred_01a1c0cb-9446-7307-afb3-57291cfcefa6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred_01a1c0cb-9446-7307-afb3-57291cfcefa6" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ApplicableTaxRate_01a1a588-1800-70c7-bdc7-0dbc7d913e12" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ApplicableTaxRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_BackgroundInformationDetailsLineItems_01a09e90-b3f0-790b-9604-9091f270ce4b" xlink:to="loc_ifrs-full_ApplicableTaxRate_01a1a588-1800-70c7-bdc7-0dbc7d913e12" xlink:type="arc" order="14"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/TradeaccountsreceivableScheduleofTradeAccountsReceivableDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#TradeaccountsreceivableScheduleofTradeAccountsReceivableDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/TradeaccountsreceivableScheduleofTradeAccountsReceivableDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems_01a0582c-fb13-793d-a3dc-a37fab47f045" xlink:href="jbsay-20260930.xsd#jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsTable_01a0582c-fb13-76bd-8d34-5f7158fd343b" xlink:href="jbsay-20260930.xsd#jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems_01a0582c-fb13-793d-a3dc-a37fab47f045" xlink:to="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsTable_01a0582c-fb13-76bd-8d34-5f7158fd343b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OverdueReceivablesAxis_01a0582c-fb13-7d7d-a8f7-4f66f19dacbe" xlink:href="jbsay-20260930.xsd#jbsay_OverdueReceivablesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsTable_01a0582c-fb13-76bd-8d34-5f7158fd343b" xlink:to="loc_jbsay_OverdueReceivablesAxis_01a0582c-fb13-7d7d-a8f7-4f66f19dacbe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-7d7d-a8f7-4f66f19dacbe_default" xlink:href="jbsay-20260930.xsd#jbsay_OverdueReceivablesDomainDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_jbsay_OverdueReceivablesAxis_01a0582c-fb13-7d7d-a8f7-4f66f19dacbe" xlink:to="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-7d7d-a8f7-4f66f19dacbe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-78d2-bc54-9eb630b6e03c" xlink:href="jbsay-20260930.xsd#jbsay_OverdueReceivablesDomainDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_jbsay_OverdueReceivablesAxis_01a0582c-fb13-7d7d-a8f7-4f66f19dacbe" xlink:to="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-78d2-bc54-9eb630b6e03c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FromOneToThirtyDaysMember_01a0582c-fb13-7a68-b973-63d7cf30ee2e" xlink:href="jbsay-20260930.xsd#jbsay_FromOneToThirtyDaysMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-78d2-bc54-9eb630b6e03c" xlink:to="loc_jbsay_FromOneToThirtyDaysMember_01a0582c-fb13-7a68-b973-63d7cf30ee2e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FromThirtyOneToSixtyDaysMember_01a0582c-fb13-7528-b0d6-fc60b6ec5b44" xlink:href="jbsay-20260930.xsd#jbsay_FromThirtyOneToSixtyDaysMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-78d2-bc54-9eb630b6e03c" xlink:to="loc_jbsay_FromThirtyOneToSixtyDaysMember_01a0582c-fb13-7528-b0d6-fc60b6ec5b44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FromSixtyOneToNinetyDaysMember_01a0582c-fb13-7765-9fe1-1448bcd1eeee" xlink:href="jbsay-20260930.xsd#jbsay_FromSixtyOneToNinetyDaysMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-78d2-bc54-9eb630b6e03c" xlink:to="loc_jbsay_FromSixtyOneToNinetyDaysMember_01a0582c-fb13-7765-9fe1-1448bcd1eeee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AboveNinetyDaysMember_01a0582c-fb13-7282-a399-8dc36e8102a1" xlink:href="jbsay-20260930.xsd#jbsay_AboveNinetyDaysMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_OverdueReceivablesDomainDomain_01a0582c-fb13-78d2-bc54-9eb630b6e03c" xlink:to="loc_jbsay_AboveNinetyDaysMember_01a0582c-fb13-7282-a399-8dc36e8102a1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentReceivablesAbstract_01a0582c-fb13-726d-b9bf-da304d8062cc" xlink:href="jbsay-20260930.xsd#jbsay_CurrentReceivablesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems_01a0582c-fb13-793d-a3dc-a37fab47f045" xlink:to="loc_jbsay_CurrentReceivablesAbstract_01a0582c-fb13-726d-b9bf-da304d8062cc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentTradeReceivable_01a0582c-fb13-72b5-b732-6715b2957ba2" xlink:href="jbsay-20260930.xsd#jbsay_CurrentTradeReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_CurrentReceivablesAbstract_01a0582c-fb13-726d-b9bf-da304d8062cc" xlink:to="loc_jbsay_CurrentTradeReceivable_01a0582c-fb13-72b5-b732-6715b2957ba2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OverdueReceivablesAbstract_01a0582c-fb13-7b3f-ba14-5500148bf5f0" xlink:href="jbsay-20260930.xsd#jbsay_OverdueReceivablesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems_01a0582c-fb13-793d-a3dc-a37fab47f045" xlink:to="loc_jbsay_OverdueReceivablesAbstract_01a0582c-fb13-7b3f-ba14-5500148bf5f0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentTradeReceivables_01a0582c-fb13-7cf7-b3cb-10acd885b37b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentTradeReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_OverdueReceivablesAbstract_01a0582c-fb13-7b3f-ba14-5500148bf5f0" xlink:to="loc_ifrs-full_NoncurrentTradeReceivables_01a0582c-fb13-7cf7-b3cb-10acd885b37b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UndiscountedExpectedCreditLossesAtInitialRecognitionOnPurchasedOrOriginatedCreditimpairedFinancialAssetsInitiallyRecognised_01a0582c-fb13-738d-b0c8-a2b8b2daed68" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_UndiscountedExpectedCreditLossesAtInitialRecognitionOnPurchasedOrOriginatedCreditimpairedFinancialAssetsInitiallyRecognised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems_01a0582c-fb13-793d-a3dc-a37fab47f045" xlink:to="loc_ifrs-full_UndiscountedExpectedCreditLossesAtInitialRecognitionOnPurchasedOrOriginatedCreditimpairedFinancialAssetsInitiallyRecognised_01a0582c-fb13-738d-b0c8-a2b8b2daed68" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TradeReceivables_01a0582c-fb13-75e2-b148-0fd7d4ad8263" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_TradeAccountsReceivableScheduleofTradeAccountsReceivableDetailsLineItems_01a0582c-fb13-793d-a3dc-a37fab47f045" xlink:to="loc_ifrs-full_TradeReceivables_01a0582c-fb13-75e2-b148-0fd7d4ad8263" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RelatedpartytransactionsScheduleofRelatedPartyReceivablesDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RelatedpartytransactionsScheduleofRelatedPartyReceivablesDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RelatedpartytransactionsScheduleofRelatedPartyReceivablesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsScheduleofRelatedPartyReceivablesDetailsTable_01a0582c-fb14-70a6-9848-918bc37a0f80" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsScheduleofRelatedPartyReceivablesDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:to="loc_jbsay_RelatedPartyTransactionsScheduleofRelatedPartyReceivablesDetailsTable_01a0582c-fb14-70a6-9848-918bc37a0f80" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fb14-7b69-ba84-bd47661b776e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RelatedPartyTransactionsScheduleofRelatedPartyReceivablesDetailsTable_01a0582c-fb14-70a6-9848-918bc37a0f80" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fb14-7b69-ba84-bd47661b776e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7b69-ba84-bd47661b776e_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fb14-7b69-ba84-bd47661b776e" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7b69-ba84-bd47661b776e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7f05-9bef-fcf5c7ab98f3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fb14-7b69-ba84-bd47661b776e" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7f05-9bef-fcf5c7ab98f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LaguzIFundoDeInvestimentoMember_01a09ee2-4f9e-7096-8ae2-693246a9dfc0" xlink:href="jbsay-20260930.xsd#jbsay_LaguzIFundoDeInvestimentoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7f05-9bef-fcf5c7ab98f3" xlink:to="loc_jbsay_LaguzIFundoDeInvestimentoMember_01a09ee2-4f9e-7096-8ae2-693246a9dfc0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_JFInvestimentosSAMember_01a0582c-fb14-7073-9bf4-8f8a5ad8e01e" xlink:href="jbsay-20260930.xsd#jbsay_JFInvestimentosSAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7f05-9bef-fcf5c7ab98f3" xlink:to="loc_jbsay_JFInvestimentosSAMember_01a0582c-fb14-7073-9bf4-8f8a5ad8e01e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FloraProdutosDeHigieneELimpezaSAMember_01a0582c-fb14-7037-9e6a-b1c6cb3f10ae" xlink:href="jbsay-20260930.xsd#jbsay_FloraProdutosDeHigieneELimpezaSAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7f05-9bef-fcf5c7ab98f3" xlink:to="loc_jbsay_FloraProdutosDeHigieneELimpezaSAMember_01a0582c-fb14-7037-9e6a-b1c6cb3f10ae" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_JBSSAJBSCompanyMember_01a09ee9-9072-79eb-8b21-d47e8c9fcb30" xlink:href="jbsay-20260930.xsd#jbsay_JBSSAJBSCompanyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fb14-7f05-9bef-fcf5c7ab98f3" xlink:to="loc_jbsay_JBSSAJBSCompanyMember_01a09ee9-9072-79eb-8b21-d47e8c9fcb30" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsAxis_01a0582c-fb14-7f9a-b1d9-010358a97338" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RelatedPartyTransactionsScheduleofRelatedPartyReceivablesDetailsTable_01a0582c-fb14-70a6-9848-918bc37a0f80" xlink:to="loc_jbsay_RelatedPartyTransactionsAxis_01a0582c-fb14-7f9a-b1d9-010358a97338" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsDomain_01a0582c-fb14-7f9a-b1d9-010358a97338_default" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_jbsay_RelatedPartyTransactionsAxis_01a0582c-fb14-7f9a-b1d9-010358a97338" xlink:to="loc_jbsay_RelatedPartyTransactionsDomain_01a0582c-fb14-7f9a-b1d9-010358a97338_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsDomain_01a0582c-fb14-7c46-9dd3-879ba14aaabf" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_jbsay_RelatedPartyTransactionsAxis_01a0582c-fb14-7f9a-b1d9-010358a97338" xlink:to="loc_jbsay_RelatedPartyTransactionsDomain_01a0582c-fb14-7c46-9dd3-879ba14aaabf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SettlementAgreementMember_01a0582c-fb14-7f95-a3a1-6d85b7968cc6" xlink:href="jbsay-20260930.xsd#jbsay_SettlementAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDomain_01a0582c-fb14-7c46-9dd3-879ba14aaabf" xlink:to="loc_jbsay_SettlementAgreementMember_01a0582c-fb14-7f95-a3a1-6d85b7968cc6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PurchaseOfAPlantMember_01a0582c-fb14-7078-9aac-2fe6c37c2a20" xlink:href="jbsay-20260930.xsd#jbsay_PurchaseOfAPlantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDomain_01a0582c-fb14-7c46-9dd3-879ba14aaabf" xlink:to="loc_jbsay_PurchaseOfAPlantMember_01a0582c-fb14-7078-9aac-2fe6c37c2a20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TradeAndOtherCurrentPayablesToRelatedParties_01a0582c-fb14-78c6-a5f5-ca60f32fc34b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherCurrentPayablesToRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:to="loc_ifrs-full_TradeAndOtherCurrentPayablesToRelatedParties_01a0582c-fb14-78c6-a5f5-ca60f32fc34b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TradeAndOtherCurrentReceivablesDueFromRelatedParties_01a0582c-fb14-7c5b-ba21-09d4cdfc40ce" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherCurrentReceivablesDueFromRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:to="loc_ifrs-full_TradeAndOtherCurrentReceivablesDueFromRelatedParties_01a0582c-fb14-7c5b-ba21-09d4cdfc40ce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetIncomeRelatedPartyTransactions_01a0582c-fb14-7d6c-96ea-be2f280cbf92" xlink:href="jbsay-20260930.xsd#jbsay_NetIncomeRelatedPartyTransactions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:to="loc_jbsay_NetIncomeRelatedPartyTransactions_01a0582c-fb14-7d6c-96ea-be2f280cbf92" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionNumberOfInstallments_01a09ee9-9072-7229-bc30-ed4802c3b739" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionNumberOfInstallments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:to="loc_jbsay_RelatedPartyTransactionNumberOfInstallments_01a09ee9-9072-7229-bc30-ed4802c3b739" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionCreditRightsDiscount_01a09ee9-9072-7a81-9b6c-f75e125ab8da" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionCreditRightsDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfTransactionsBetweenRelatedPartiesLineItems_01a0582c-fb14-740a-ba93-600d89a466dc" xlink:to="loc_jbsay_RelatedPartyTransactionCreditRightsDiscount_01a09ee9-9072-7a81-9b6c-f75e125ab8da" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RelatedpartytransactionsNarrativeDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RelatedpartytransactionsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RelatedpartytransactionsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsDetailsTable_01a0582c-fb14-73ff-ad02-fcc8120b870a" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:to="loc_jbsay_RelatedPartyTransactionsDetailsTable_01a0582c-fb14-73ff-ad02-fcc8120b870a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfAntidilutiveInstrumentsAxis_01a09ee7-7374-7859-a3ed-ffee44bbc648" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsTable_01a0582c-fb14-73ff-ad02-fcc8120b870a" xlink:to="loc_ifrs-full_TypesOfAntidilutiveInstrumentsAxis_01a09ee7-7374-7859-a3ed-ffee44bbc648" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfAntidilutiveInstrumentsDomain_01a09ee7-7374-7859-a3ed-ffee44bbc648_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TypesOfAntidilutiveInstrumentsAxis_01a09ee7-7374-7859-a3ed-ffee44bbc648" xlink:to="loc_ifrs-full_TypesOfAntidilutiveInstrumentsDomain_01a09ee7-7374-7859-a3ed-ffee44bbc648_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TypesOfAntidilutiveInstrumentsDomain_01a09ee7-7374-7ed3-ab89-769f61b62ff7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TypesOfAntidilutiveInstrumentsAxis_01a09ee7-7374-7859-a3ed-ffee44bbc648" xlink:to="loc_ifrs-full_TypesOfAntidilutiveInstrumentsDomain_01a09ee7-7374-7ed3-ab89-769f61b62ff7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CertificadoDeDepsitoInterbancrioMember_01a09ee7-7374-7658-a66c-13ead3c2cf54" xlink:href="jbsay-20260930.xsd#jbsay_CertificadoDeDepsitoInterbancrioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TypesOfAntidilutiveInstrumentsDomain_01a09ee7-7374-7ed3-ab89-769f61b62ff7" xlink:to="loc_jbsay_CertificadoDeDepsitoInterbancrioMember_01a09ee7-7374-7658-a66c-13ead3c2cf54" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AssignedReceivables_01a09ee7-7374-7987-b80a-ac310ef9e200" xlink:href="jbsay-20260930.xsd#jbsay_AssignedReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:to="loc_jbsay_AssignedReceivables_01a09ee7-7374-7987-b80a-ac310ef9e200" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LossFromSaleOfReceivables_01a09ee7-7374-72c4-911a-d0847b19a973" xlink:href="jbsay-20260930.xsd#jbsay_LossFromSaleOfReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:to="loc_jbsay_LossFromSaleOfReceivables_01a09ee7-7374-72c4-911a-d0847b19a973" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentInvestments_01a09ee7-7374-7eea-9b59-24fa8e0043aa" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:to="loc_ifrs-full_CurrentInvestments_01a09ee7-7374-7eea-9b59-24fa8e0043aa" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RevenueFromInterest_01a09ee7-7374-744e-9928-07d5d73eaae8" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RevenueFromInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:to="loc_ifrs-full_RevenueFromInterest_01a09ee7-7374-744e-9928-07d5d73eaae8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ContractualCapitalCommitments_01a09ee7-7374-7f10-89b8-58b78f07e6fe" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ContractualCapitalCommitments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RelatedPartyTransactionsDetailsLineItems_01a09ee7-7374-73ec-b719-39a9a6211d53" xlink:to="loc_ifrs-full_ContractualCapitalCommitments_01a09ee7-7374-7f10-89b8-58b78f07e6fe" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/IncometaxesScheduleofDeferredTaxIncomeandSocialContributionDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#IncometaxesScheduleofDeferredTaxIncomeandSocialContributionDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/IncometaxesScheduleofDeferredTaxIncomeandSocialContributionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeferredIncomeTaxLineItems_01a0582c-fb14-7358-bf9d-16fd26367647" xlink:href="jbsay-20260930.xsd#jbsay_DeferredIncomeTaxLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncomeTaxesScheduleofDeferredTaxIncomeandSocialContributionDetailsTable_01a0582c-fb14-74d0-9bd5-aa0f40ed2fa3" xlink:href="jbsay-20260930.xsd#jbsay_IncomeTaxesScheduleofDeferredTaxIncomeandSocialContributionDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_DeferredIncomeTaxLineItems_01a0582c-fb14-7358-bf9d-16fd26367647" xlink:to="loc_jbsay_IncomeTaxesScheduleofDeferredTaxIncomeandSocialContributionDetailsTable_01a0582c-fb14-74d0-9bd5-aa0f40ed2fa3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_01a0582c-fb14-794c-8cb3-2a9d4ac71f9e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_IncomeTaxesScheduleofDeferredTaxIncomeandSocialContributionDetailsTable_01a0582c-fb14-74d0-9bd5-aa0f40ed2fa3" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_01a0582c-fb14-794c-8cb3-2a9d4ac71f9e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-794c-8cb3-2a9d4ac71f9e_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_01a0582c-fb14-794c-8cb3-2a9d4ac71f9e" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-794c-8cb3-2a9d4ac71f9e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsAxis_01a0582c-fb14-794c-8cb3-2a9d4ac71f9e" xlink:to="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TaxLossAndNegativeSocialContributionBaseMember_01a0582c-fb14-7fb4-bd8a-86c479b6e298" xlink:href="jbsay-20260930.xsd#jbsay_TaxLossAndNegativeSocialContributionBaseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_TaxLossAndNegativeSocialContributionBaseMember_01a0582c-fb14-7fb4-bd8a-86c479b6e298" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExpectedCreditLossesOnTradeAccountsReceivableMember_01a0582c-fb14-7f98-9f39-8e8b218bbc6b" xlink:href="jbsay-20260930.xsd#jbsay_ExpectedCreditLossesOnTradeAccountsReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_ExpectedCreditLossesOnTradeAccountsReceivableMember_01a0582c-fb14-7f98-9f39-8e8b218bbc6b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProvisionsForContingenciesMember_01a0582c-fb14-777b-b58d-3282192259a1" xlink:href="jbsay-20260930.xsd#jbsay_ProvisionsForContingenciesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_ProvisionsForContingenciesMember_01a0582c-fb14-777b-b58d-3282192259a1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FairValueAdjustmentMember_01a0582c-fb14-77f4-8813-774c7b0d3e55" xlink:href="jbsay-20260930.xsd#jbsay_FairValueAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_FairValueAdjustmentMember_01a0582c-fb14-77f4-8813-774c7b0d3e55" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TaxCreditsForeignSubsidiariesMember_01a0582c-fb14-7f1e-acff-5d8d31eaa771" xlink:href="jbsay-20260930.xsd#jbsay_TaxCreditsForeignSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_TaxCreditsForeignSubsidiariesMember_01a0582c-fb14-7f1e-acff-5d8d31eaa771" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ShareBasedPaymentMember_01a0582c-fb14-76dc-bc06-7340cb3dbd1d" xlink:href="jbsay-20260930.xsd#jbsay_ShareBasedPaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_ShareBasedPaymentMember_01a0582c-fb14-76dc-bc06-7340cb3dbd1d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProvisionForWorkAccidentInsuranceForeignSubsidiariesMember_01a0582c-fb14-75b2-818a-4619d97df3a0" xlink:href="jbsay-20260930.xsd#jbsay_ProvisionForWorkAccidentInsuranceForeignSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_ProvisionForWorkAccidentInsuranceForeignSubsidiariesMember_01a0582c-fb14-75b2-818a-4619d97df3a0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PensionPlanForeignSubsidiariesMember_01a0582c-fb14-7e3f-9e0d-cec99fb40324" xlink:href="jbsay-20260930.xsd#jbsay_PensionPlanForeignSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_PensionPlanForeignSubsidiariesMember_01a0582c-fb14-7e3f-9e0d-cec99fb40324" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TradeAccountsPayableAccrualMember_01a0582c-fb14-7361-93b3-7f189f337240" xlink:href="jbsay-20260930.xsd#jbsay_TradeAccountsPayableAccrualMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_TradeAccountsPayableAccrualMember_01a0582c-fb14-7361-93b3-7f189f337240" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonDeductibleInterestPortionMember_01a0582c-fb14-77af-82b8-af7da92b15b7" xlink:href="jbsay-20260930.xsd#jbsay_NonDeductibleInterestPortionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_NonDeductibleInterestPortionMember_01a0582c-fb14-77af-82b8-af7da92b15b7" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RightofuseAssetsMember_01a0582c-fb14-7e15-8d1a-4bee55eb0f87" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RightofuseAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_ifrs-full_RightofuseAssetsMember_01a0582c-fb14-7e15-8d1a-4bee55eb0f87" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_GoodwillAmortizationMember_01a0582c-fb14-7e4b-8b43-117e1a2d4e7a" xlink:href="jbsay-20260930.xsd#jbsay_GoodwillAmortizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_GoodwillAmortizationMember_01a0582c-fb14-7e4b-8b43-117e1a2d4e7a" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BusinessCombinationMember_01a0582c-fb14-71a0-b1c3-108cc66e22ff" xlink:href="jbsay-20260930.xsd#jbsay_BusinessCombinationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_BusinessCombinationMember_01a0582c-fb14-71a0-b1c3-108cc66e22ff" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_InventoryValuationMember_01a0582c-fb14-7f02-a72a-111c53b951f1" xlink:href="jbsay-20260930.xsd#jbsay_InventoryValuationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_InventoryValuationMember_01a0582c-fb14-7f02-a72a-111c53b951f1" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_HedgeOperationsMember_01a0582c-fb14-74ac-9247-9610025491e9" xlink:href="jbsay-20260930.xsd#jbsay_HedgeOperationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_HedgeOperationsMember_01a0582c-fb14-74ac-9247-9610025491e9" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RealizationOfOtherReservesMember_01a0582c-fb14-766c-9f83-7c17fb4b5240" xlink:href="jbsay-20260930.xsd#jbsay_RealizationOfOtherReservesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_RealizationOfOtherReservesMember_01a0582c-fb14-766c-9f83-7c17fb4b5240" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AcceleratedDepreciationAndAmortizationMember_01a0582c-fb14-7a67-93ae-4a3549cd2d04" xlink:href="jbsay-20260930.xsd#jbsay_AcceleratedDepreciationAndAmortizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_AcceleratedDepreciationAndAmortizationMember_01a0582c-fb14-7a67-93ae-4a3549cd2d04" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CutOffAdjustmentssalesMember_01a0582c-fb14-7993-98cd-ac9c575abd23" xlink:href="jbsay-20260930.xsd#jbsay_CutOffAdjustmentssalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_CutOffAdjustmentssalesMember_01a0582c-fb14-7993-98cd-ac9c575abd23" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherTemporaryDifferencesMember_01a0582c-fb14-7bd5-87fc-032c37fb6ab9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherTemporaryDifferencesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_ifrs-full_OtherTemporaryDifferencesMember_01a0582c-fb14-7bd5-87fc-032c37fb6ab9" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeferredTaxesNetMember_01a0582c-fb14-74e9-aebe-6a9d1ed9de8e" xlink:href="jbsay-20260930.xsd#jbsay_DeferredTaxesNetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_TemporaryDifferenceUnusedTaxLossesAndUnusedTaxCreditsDomain_01a0582c-fb14-7581-bb55-9d52590052bb" xlink:to="loc_jbsay_DeferredTaxesNetMember_01a0582c-fb14-74e9-aebe-6a9d1ed9de8e" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NetDeferredTaxAssets_01a0582c-fb14-7643-b6ea-76becacaee39" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NetDeferredTaxAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DeferredIncomeTaxLineItems_01a0582c-fb14-7358-bf9d-16fd26367647" xlink:to="loc_ifrs-full_NetDeferredTaxAssets_01a0582c-fb14-7643-b6ea-76becacaee39" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxExpenseIncomeRecognisedInProfitOrLoss_01a0582c-fb14-7775-824e-ee1dd525a7ea" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxExpenseIncomeRecognisedInProfitOrLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DeferredIncomeTaxLineItems_01a0582c-fb14-7358-bf9d-16fd26367647" xlink:to="loc_ifrs-full_DeferredTaxExpenseIncomeRecognisedInProfitOrLoss_01a0582c-fb14-7775-824e-ee1dd525a7ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeferredTaxAssetExchangeRateVariation_01a0582c-fb14-75c4-bf04-6e2d2d8dadbc" xlink:href="jbsay-20260930.xsd#jbsay_DeferredTaxAssetExchangeRateVariation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DeferredIncomeTaxLineItems_01a0582c-fb14-7358-bf9d-16fd26367647" xlink:to="loc_jbsay_DeferredTaxAssetExchangeRateVariation_01a0582c-fb14-75c4-bf04-6e2d2d8dadbc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeferredTaxOtherAdjustments_01a0582c-fb14-78ca-9ccc-3f408dae2efd" xlink:href="jbsay-20260930.xsd#jbsay_DeferredTaxOtherAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DeferredIncomeTaxLineItems_01a0582c-fb14-7358-bf9d-16fd26367647" xlink:to="loc_jbsay_DeferredTaxOtherAdjustments_01a0582c-fb14-78ca-9ccc-3f408dae2efd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NetDeferredTaxAssets_01a0582c-fb14-75d5-85fb-97d3ae8b4196" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NetDeferredTaxAssets"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/InvestmentsinequityaccountedinvesteesassociatesandjointventureDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#InvestmentsinequityaccountedinvesteesassociatesandjointventureDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/InvestmentsinequityaccountedinvesteesassociatesandjointventureDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:href="jbsay-20260930.xsd#jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesTable_01a0582c-fb14-7b1d-8751-d5832e704c60" xlink:href="jbsay-20260930.xsd#jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesTable_01a0582c-fb14-7b1d-8751-d5832e704c60" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_JointVenturesAxis_01a0582c-fb14-7269-8484-e4c86c947f56" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_JointVenturesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesTable_01a0582c-fb14-7b1d-8751-d5832e704c60" xlink:to="loc_ifrs-full_JointVenturesAxis_01a0582c-fb14-7269-8484-e4c86c947f56" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_JointVenturesDomain_01a0582c-fb14-7269-8484-e4c86c947f56_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_JointVenturesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_JointVenturesAxis_01a0582c-fb14-7269-8484-e4c86c947f56" xlink:to="loc_ifrs-full_JointVenturesDomain_01a0582c-fb14-7269-8484-e4c86c947f56_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_JointVenturesDomain_01a0582c-fb14-7aae-a2b3-00f902b57caa" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_JointVenturesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_JointVenturesAxis_01a0582c-fb14-7269-8484-e4c86c947f56" xlink:to="loc_ifrs-full_JointVenturesDomain_01a0582c-fb14-7aae-a2b3-00f902b57caa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MeatSnacksPartnersDoBrasilLtdaMeatSnacksMember_01a0582c-fb14-77cf-b313-e2016f96013a" xlink:href="jbsay-20260930.xsd#jbsay_MeatSnacksPartnersDoBrasilLtdaMeatSnacksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_JointVenturesDomain_01a0582c-fb14-7aae-a2b3-00f902b57caa" xlink:to="loc_jbsay_MeatSnacksPartnersDoBrasilLtdaMeatSnacksMember_01a0582c-fb14-77cf-b313-e2016f96013a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_01a0582c-fb14-707a-a060-5f17f798b822" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesTable_01a0582c-fb14-7b1d-8751-d5832e704c60" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_01a0582c-fb14-707a-a060-5f17f798b822" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-707a-a060-5f17f798b822_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_01a0582c-fb14-707a-a060-5f17f798b822" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-707a-a060-5f17f798b822_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-770d-994c-90b76e792011" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SignificantInvestmentsInAssociatesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesAxis_01a0582c-fb14-707a-a060-5f17f798b822" xlink:to="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-770d-994c-90b76e792011" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_JBSOntarioMember_01a0582c-fb14-74c6-8c54-7de626ec95df" xlink:href="jbsay-20260930.xsd#jbsay_JBSOntarioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-770d-994c-90b76e792011" xlink:to="loc_jbsay_JBSOntarioMember_01a0582c-fb14-74c6-8c54-7de626ec95df" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BirlaSocietaAgricolaSrlMember_01a0582c-fb14-7c99-903a-12b041276f0b" xlink:href="jbsay-20260930.xsd#jbsay_BirlaSocietaAgricolaSrlMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-770d-994c-90b76e792011" xlink:to="loc_jbsay_BirlaSocietaAgricolaSrlMember_01a0582c-fb14-7c99-903a-12b041276f0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MantiqueiraAlimentosS.A.Member_01a0582c-fb14-7280-b954-9bf8b42deff7" xlink:href="jbsay-20260930.xsd#jbsay_MantiqueiraAlimentosS.A.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-770d-994c-90b76e792011" xlink:to="loc_jbsay_MantiqueiraAlimentosS.A.Member_01a0582c-fb14-7280-b954-9bf8b42deff7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MantiqueiraInternationalB.V.Member_01a0582c-fb14-723c-af93-d9b83ea1e553" xlink:href="jbsay-20260930.xsd#jbsay_MantiqueiraInternationalB.V.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SignificantInvestmentsInAssociatesDomain_01a0582c-fb14-770d-994c-90b76e792011" xlink:to="loc_jbsay_MantiqueiraInternationalB.V.Member_01a0582c-fb14-723c-af93-d9b83ea1e553" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProportionOfOwnershipInterestInJointVenture_01a0582c-fb14-7f07-9440-05a03f1d1700" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProportionOfOwnershipInterestInJointVenture"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_ifrs-full_ProportionOfOwnershipInterestInJointVenture_01a0582c-fb14-7f07-9440-05a03f1d1700" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProportionOfOwnershipInterestInAssociate_01a0582c-fb14-737f-9aee-aa4c61b89797" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProportionOfOwnershipInterestInAssociate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_ifrs-full_ProportionOfOwnershipInterestInAssociate_01a0582c-fb14-737f-9aee-aa4c61b89797" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InvestmentAccountedForUsingEquityMethod_01a0582c-fb14-7b84-8d69-499bff449c57" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InvestmentAccountedForUsingEquityMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_ifrs-full_InvestmentAccountedForUsingEquityMethod_01a0582c-fb14-7b84-8d69-499bff449c57" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_InvestmentsAccountedForUsingEquityMethodAdjusted_01a0582c-fb14-70f9-8a48-93f3d033bdac" xlink:href="jbsay-20260930.xsd#jbsay_InvestmentsAccountedForUsingEquityMethodAdjusted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_jbsay_InvestmentsAccountedForUsingEquityMethodAdjusted_01a0582c-fb14-70f9-8a48-93f3d033bdac" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnDisposalsOfInvestments_01a0582c-fb14-7248-aaa8-07223261be44" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnDisposalsOfInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_ifrs-full_GainsLossesOnDisposalsOfInvestments_01a0582c-fb14-7248-aaa8-07223261be44" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProfitDistributionOnInvestments_01a0582c-fb14-7f1c-b52c-9a0b7c26823d" xlink:href="jbsay-20260930.xsd#jbsay_ProfitDistributionOnInvestments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_jbsay_ProfitDistributionOnInvestments_01a0582c-fb14-7f1c-b52c-9a0b7c26823d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExchangeVariation_01a0582c-fb14-7f5d-bbbe-f452524e6d4f" xlink:href="jbsay-20260930.xsd#jbsay_ExchangeVariation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_jbsay_ExchangeVariation_01a0582c-fb14-7f5d-bbbe-f452524e6d4f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ChangeInEquityOfInvesteesEquityMethod_01a0582c-fb14-71b8-bc61-51bb702cef25" xlink:href="jbsay-20260930.xsd#jbsay_ChangeInEquityOfInvesteesEquityMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_jbsay_ChangeInEquityOfInvesteesEquityMethod_01a0582c-fb14-71b8-bc61-51bb702cef25" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProportionateShareOfIncomeEquityMethod_01a0582c-fb14-7278-976b-cf03f98f2622" xlink:href="jbsay-20260930.xsd#jbsay_ProportionateShareOfIncomeEquityMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_jbsay_ProportionateShareOfIncomeEquityMethod_01a0582c-fb14-7278-976b-cf03f98f2622" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InvestmentAccountedForUsingEquityMethod_01a0582c-fb14-719e-b3ac-32ddecb7d503" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InvestmentAccountedForUsingEquityMethod"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_InvestmentsAccountedForUsingEquityMethodAdjusted_01a0582c-fb14-7f49-a264-d3e95487eef7" xlink:href="jbsay-20260930.xsd#jbsay_InvestmentsAccountedForUsingEquityMethodAdjusted"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InvestmentsInJointVenturesAccountedForUsingEquityMethod_01a0582c-fb14-77f7-9659-92c638dc00cc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InvestmentsInJointVenturesAccountedForUsingEquityMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_DisclosureOfInvestmentsInAssociatesAndJointVenturesLineItems_01a0582c-fb14-739a-bb94-8db400560453" xlink:to="loc_ifrs-full_InvestmentsInJointVenturesAccountedForUsingEquityMethod_01a0582c-fb14-77f7-9659-92c638dc00cc" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/PropertyplantandequipmentScheduleofChangesinPropertyPlantandEquipmentDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#PropertyplantandequipmentScheduleofChangesinPropertyPlantandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/PropertyplantandequipmentScheduleofChangesinPropertyPlantandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PropertyPlantandEquipmentScheduleofChangesinPropertyPlantandEquipmentDetailsTable_01a0582c-fb14-7abb-96a4-cd95f46669f5" xlink:href="jbsay-20260930.xsd#jbsay_PropertyPlantandEquipmentScheduleofChangesinPropertyPlantandEquipmentDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:to="loc_jbsay_PropertyPlantandEquipmentScheduleofChangesinPropertyPlantandEquipmentDetailsTable_01a0582c-fb14-7abb-96a4-cd95f46669f5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_01a0582c-fb14-7d11-87ee-8229c31d6114" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_PropertyPlantandEquipmentScheduleofChangesinPropertyPlantandEquipmentDetailsTable_01a0582c-fb14-7abb-96a4-cd95f46669f5" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_01a0582c-fb14-7d11-87ee-8229c31d6114" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7d11-87ee-8229c31d6114_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_01a0582c-fb14-7d11-87ee-8229c31d6114" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7d11-87ee-8229c31d6114_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentAxis_01a0582c-fb14-7d11-87ee-8229c31d6114" xlink:to="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BuildingsMember_01a0582c-fb14-7c60-b226-cbfc9e46169a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_BuildingsMember_01a0582c-fb14-7c60-b226-cbfc9e46169a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LandMember_01a0582c-fb14-7e12-b7ea-51ed0631bdeb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_LandMember_01a0582c-fb14-7e12-b7ea-51ed0631bdeb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MachineryMember_01a0582c-fb14-7a23-b38e-f2cb54ab8735" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MachineryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_MachineryMember_01a0582c-fb14-7a23-b38e-f2cb54ab8735" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FacilitiesMember_01a0582c-fb14-724c-85d9-65ad29b2bddd" xlink:href="jbsay-20260930.xsd#jbsay_FacilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_jbsay_FacilitiesMember_01a0582c-fb14-724c-85d9-65ad29b2bddd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComputerEquipmentMember_01a0582c-fb14-7f4e-88ea-ef3cfcab0ae1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_ComputerEquipmentMember_01a0582c-fb14-7f4e-88ea-ef3cfcab0ae1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_VehiclesMember_01a0582c-fb14-79c8-91ae-9f1be18f2439" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_VehiclesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_VehiclesMember_01a0582c-fb14-79c8-91ae-9f1be18f2439" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConstructionInProgressMember_01a0582c-fb14-7176-8964-6de53a2d85d5" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConstructionInProgressMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_ConstructionInProgressMember_01a0582c-fb14-7176-8964-6de53a2d85d5" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherPropertyPlantAndEquipmentMember_01a0582c-fb14-745a-b6d8-97a7496a9427" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherPropertyPlantAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfPropertyPlantAndEquipmentDomain_01a0582c-fb14-7cf3-9b93-7d861c5b8470" xlink:to="loc_ifrs-full_OtherPropertyPlantAndEquipmentMember_01a0582c-fb14-745a-b6d8-97a7496a9427" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PropertyPlantAndEquipment_01a0582c-fb14-79cb-bcb0-92fb6301718e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:to="loc_ifrs-full_PropertyPlantAndEquipment_01a0582c-fb14-79cb-bcb0-92fb6301718e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdditionsOtherThanThroughBusinessCombinationsPropertyPlantAndEquipments_01a0582c-fb14-7410-aa7a-40acf6692044" xlink:href="jbsay-20260930.xsd#jbsay_AdditionsOtherThanThroughBusinessCombinationsPropertyPlantAndEquipments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:to="loc_jbsay_AdditionsOtherThanThroughBusinessCombinationsPropertyPlantAndEquipments_01a0582c-fb14-7410-aa7a-40acf6692044" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisposalsPropertyPlantAndEquipment_01a0582c-fb14-72ea-bb41-83352fae95dd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisposalsPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:to="loc_ifrs-full_DisposalsPropertyPlantAndEquipment_01a0582c-fb14-72ea-bb41-83352fae95dd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DepreciationPropertyPlantAndEquipment_01a0582c-fb14-7477-9d21-2665f9a221f2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DepreciationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:to="loc_ifrs-full_DepreciationPropertyPlantAndEquipment_01a0582c-fb14-7477-9d21-2665f9a221f2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesPropertyPlantAndEquipment_01a0582c-fb14-7423-bbd1-167b2a64b4d5" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfPropertyPlantAndEquipmentLineItems_01a0582c-fb14-7a27-8970-6eba63710d93" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesPropertyPlantAndEquipment_01a0582c-fb14-7423-bbd1-167b2a64b4d5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PropertyPlantAndEquipment_01a0582c-fb14-7c21-94c8-1c881ffe27b1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PropertyPlantAndEquipment"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/LeasesScheduleofChangesintheRightofUseAssetDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#LeasesScheduleofChangesintheRightofUseAssetDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/LeasesScheduleofChangesintheRightofUseAssetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:href="jbsay-20260930.xsd#jbsay_ScheduleOfChangesInRightOfUseAssetLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LeasesScheduleofChangesintheRightofUseAssetDetailsTable_01a0582c-fb14-7e2a-9bc4-d4ec81d93ab3" xlink:href="jbsay-20260930.xsd#jbsay_LeasesScheduleofChangesintheRightofUseAssetDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_jbsay_LeasesScheduleofChangesintheRightofUseAssetDetailsTable_01a0582c-fb14-7e2a-9bc4-d4ec81d93ab3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsAxis_01a0582c-fb14-71da-9cef-f040bf246e08" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_LeasesScheduleofChangesintheRightofUseAssetDetailsTable_01a0582c-fb14-7e2a-9bc4-d4ec81d93ab3" xlink:to="loc_ifrs-full_ClassesOfAssetsAxis_01a0582c-fb14-71da-9cef-f040bf246e08" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-71da-9cef-f040bf246e08_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_01a0582c-fb14-71da-9cef-f040bf246e08" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-71da-9cef-f040bf246e08_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfAssetsAxis_01a0582c-fb14-71da-9cef-f040bf246e08" xlink:to="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_GrowingFacilitiesMember_01a0582c-fb14-7ced-b86d-fc9c30b49eba" xlink:href="jbsay-20260930.xsd#jbsay_GrowingFacilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_jbsay_GrowingFacilitiesMember_01a0582c-fb14-7ced-b86d-fc9c30b49eba" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BuildingsMember_01a0582c-fb14-7167-8b45-7b88921f71c0" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BuildingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_ifrs-full_BuildingsMember_01a0582c-fb14-7167-8b45-7b88921f71c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComputerEquipmentMember_01a0582c-fb14-7f73-931c-b6aa03a86704" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_ifrs-full_ComputerEquipmentMember_01a0582c-fb14-7f73-931c-b6aa03a86704" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MachineryAndEquipmentsMember_01a0582c-fb14-7eec-abd0-0a2bc90c3eea" xlink:href="jbsay-20260930.xsd#jbsay_MachineryAndEquipmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_jbsay_MachineryAndEquipmentsMember_01a0582c-fb14-7eec-abd0-0a2bc90c3eea" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingPlantsMember_01a0582c-fb14-7b76-91cf-08091447a769" xlink:href="jbsay-20260930.xsd#jbsay_OperatingPlantsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_jbsay_OperatingPlantsMember_01a0582c-fb14-7b76-91cf-08091447a769" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LandMember_01a0582c-fb14-74d0-9684-9fd658e6f595" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_ifrs-full_LandMember_01a0582c-fb14-74d0-9684-9fd658e6f595" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_VehiclesMember_01a0582c-fb14-7a5b-aeec-a3c7f66272e4" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_VehiclesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_ifrs-full_VehiclesMember_01a0582c-fb14-7a5b-aeec-a3c7f66272e4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ConcessionAgreementMember_01a0582c-fb14-7cdc-8b02-522a0ed2b554" xlink:href="jbsay-20260930.xsd#jbsay_ConcessionAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfAssetsDomain_01a0582c-fb14-7901-9c43-79b809f8d21d" xlink:to="loc_jbsay_ConcessionAgreementMember_01a0582c-fb14-7cdc-8b02-522a0ed2b554" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RightofuseAssets_01a0582c-fb14-79ce-aca0-96364ec0014d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RightofuseAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_ifrs-full_RightofuseAssets_01a0582c-fb14-79ce-aca0-96364ec0014d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdditionsToRightOfUseAsset_01a0582c-fb14-7229-87a5-8b301811ceaa" xlink:href="jbsay-20260930.xsd#jbsay_AdditionsToRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_jbsay_AdditionsToRightOfUseAsset_01a0582c-fb14-7229-87a5-8b301811ceaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TerminatedContracts_01a0582c-fb14-73b3-97db-125e7af38814" xlink:href="jbsay-20260930.xsd#jbsay_TerminatedContracts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_jbsay_TerminatedContracts_01a0582c-fb14-73b3-97db-125e7af38814" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AmortizationRightOfUseAsset_01a0582c-fb14-7e28-bcf5-2d48959019e2" xlink:href="jbsay-20260930.xsd#jbsay_AmortizationRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_jbsay_AmortizationRightOfUseAsset_01a0582c-fb14-7e28-bcf5-2d48959019e2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExchangeRateVariationRightOfUseAsset_01a0582c-fb14-7408-891e-9deb961db77b" xlink:href="jbsay-20260930.xsd#jbsay_ExchangeRateVariationRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_jbsay_ExchangeRateVariationRightOfUseAsset_01a0582c-fb14-7408-891e-9deb961db77b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RightofuseAssets_01a0582c-fb14-7a89-ae00-0be54af9b2eb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RightofuseAssets"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TaxImpactOfAdditionsToRightOfUseAsset_01a0582c-fb14-700c-9a1e-433f3635ac32" xlink:href="jbsay-20260930.xsd#jbsay_TaxImpactOfAdditionsToRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfChangesInRightOfUseAssetLineItems_01a0582c-fb14-707b-8100-e4c44b158afd" xlink:to="loc_jbsay_TaxImpactOfAdditionsToRightOfUseAsset_01a0582c-fb14-700c-9a1e-433f3635ac32" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/IntangibleassetsDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#IntangibleassetsDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/IntangibleassetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsLineItems_01a0582c-fc0a-731d-8e34-9ffa0d35b8dc" xlink:href="jbsay-20260930.xsd#jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsTable_01a0582c-fc0a-7ff4-b6e8-5e433a0f037d" xlink:href="jbsay-20260930.xsd#jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsLineItems_01a0582c-fc0a-731d-8e34-9ffa0d35b8dc" xlink:to="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsTable_01a0582c-fc0a-7ff4-b6e8-5e433a0f037d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_01a0582c-fc0a-744c-b333-0744f9dc61ed" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsTable_01a0582c-fc0a-7ff4-b6e8-5e433a0f037d" xlink:to="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_01a0582c-fc0a-744c-b333-0744f9dc61ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_01a0582c-fc0a-744c-b333-0744f9dc61ed_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_01a0582c-fc0a-744c-b333-0744f9dc61ed" xlink:to="loc_ifrs-full_CarryingAmountMember_01a0582c-fc0a-744c-b333-0744f9dc61ed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CarryingAmountMember_01a0582c-fc0a-7b6c-9f94-3eccd969656c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CarryingAmountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CarryingAmountAccumulatedDepreciationAmortisationAndImpairmentAndGrossCarryingAmountAxis_01a0582c-fc0a-744c-b333-0744f9dc61ed" xlink:to="loc_ifrs-full_CarryingAmountMember_01a0582c-fc0a-7b6c-9f94-3eccd969656c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AmortizingMember_01a0582c-fc0a-78ed-a13f-3df1fd314426" xlink:href="jbsay-20260930.xsd#jbsay_AmortizingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_01a0582c-fc0a-7b6c-9f94-3eccd969656c" xlink:to="loc_jbsay_AmortizingMember_01a0582c-fc0a-78ed-a13f-3df1fd314426" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonAmortizingMember_01a0582c-fc0a-72fb-85c7-03a2ede61846" xlink:href="jbsay-20260930.xsd#jbsay_NonAmortizingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CarryingAmountMember_01a0582c-fc0a-7b6c-9f94-3eccd969656c" xlink:to="loc_jbsay_NonAmortizingMember_01a0582c-fc0a-72fb-85c7-03a2ede61846" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_01a0582c-fc0a-7ea1-a7b4-999f58b6aa5c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsTable_01a0582c-fc0a-7ff4-b6e8-5e433a0f037d" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_01a0582c-fc0a-7ea1-a7b4-999f58b6aa5c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7ea1-a7b4-999f58b6aa5c_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_01a0582c-fc0a-7ea1-a7b4-999f58b6aa5c" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7ea1-a7b4-999f58b6aa5c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillAxis_01a0582c-fc0a-7ea1-a7b4-999f58b6aa5c" xlink:to="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TrademarkMember_01a0582c-fc0a-7bf9-804a-86e3d43385a2" xlink:href="jbsay-20260930.xsd#jbsay_TrademarkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_jbsay_TrademarkMember_01a0582c-fc0a-7bf9-804a-86e3d43385a2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComputerSoftwareMember_01a0582c-fc0a-7cb4-b893-804322288bfd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComputerSoftwareMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_ifrs-full_ComputerSoftwareMember_01a0582c-fc0a-7cb4-b893-804322288bfd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CustomerRelationshipMember_01a0582c-fc0a-7e56-a173-08dd7d66a528" xlink:href="jbsay-20260930.xsd#jbsay_CustomerRelationshipMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_jbsay_CustomerRelationshipMember_01a0582c-fc0a-7e56-a173-08dd7d66a528" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SupplierContractsMember_01a0582c-fc0a-729e-8bb5-69e8c760f29b" xlink:href="jbsay-20260930.xsd#jbsay_SupplierContractsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_jbsay_SupplierContractsMember_01a0582c-fc0a-729e-8bb5-69e8c760f29b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ServiceConcessionRightsMember_01a0582c-fc0a-73cf-9ec6-f236938958c2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ServiceConcessionRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_ifrs-full_ServiceConcessionRightsMember_01a0582c-fc0a-73cf-9ec6-f236938958c2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OthersMember_01a0582c-fc0a-716a-8e18-c8b286bc3406" xlink:href="jbsay-20260930.xsd#jbsay_OthersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_jbsay_OthersMember_01a0582c-fc0a-716a-8e18-c8b286bc3406" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WaterRightsMember_01a0582c-fc0a-7119-b73f-efa3e3984149" xlink:href="jbsay-20260930.xsd#jbsay_WaterRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_jbsay_WaterRightsMember_01a0582c-fc0a-7119-b73f-efa3e3984149" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IntangibleAssetsMember_01a0582c-fc0a-7938-a043-7f79ca8e5f93" xlink:href="jbsay-20260930.xsd#jbsay_IntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfIntangibleAssetsOtherThanGoodwillDomain_01a0582c-fc0a-7c13-bfb6-9b895fa00bdc" xlink:to="loc_jbsay_IntangibleAssetsMember_01a0582c-fc0a-7938-a043-7f79ca8e5f93" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:href="jbsay-20260930.xsd#jbsay_AmortizingAndNonAmortizingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_IntangibleAssetsScheduleofChangesinIntangibleAssetsDetailsLineItems_01a0582c-fc0a-731d-8e34-9ffa0d35b8dc" xlink:to="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7846-8bb8-97793d191935" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:to="loc_ifrs-full_IntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7846-8bb8-97793d191935" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7c0b-abd8-5cec8a1cf41f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:to="loc_ifrs-full_AdditionsOtherThanThroughBusinessCombinationsIntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7c0b-abd8-5cec8a1cf41f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DisposalsIntangibleAssets_01a0582c-fc0a-715b-b988-94d574d1a377" xlink:href="jbsay-20260930.xsd#jbsay_DisposalsIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:to="loc_jbsay_DisposalsIntangibleAssets_01a0582c-fc0a-715b-b988-94d574d1a377" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AmortisationIntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-75c1-903b-90fe49c1b847" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AmortisationIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:to="loc_ifrs-full_AmortisationIntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-75c1-903b-90fe49c1b847" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7e05-950d-7cf002326c45" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_AmortizingAndNonAmortizingAbstract_01a0582c-fc0a-71e4-9f2e-63e0ca7cbb0e" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesIntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7e05-950d-7cf002326c45" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsOtherThanGoodwill_01a0582c-fc0a-7326-9f69-ee3893ec3a9d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IntangibleAssetsOtherThanGoodwill"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/LoansandfinancingScheduleofLoansandFinancingDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#LoansandfinancingScheduleofLoansandFinancingDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/LoansandfinancingScheduleofLoansandFinancingDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSummarisedFinancialInformationAboutForeignOperationLineItems_01a0582c-fc0a-7eb8-a0b3-4b012219b271" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSummarisedFinancialInformationAboutForeignOperationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LoansandFinancingScheduleofLoansandFinancingDetailsTable_01a0582c-fc0a-7126-9bb8-4ed37854b0b0" xlink:href="jbsay-20260930.xsd#jbsay_LoansandFinancingScheduleofLoansandFinancingDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSummarisedFinancialInformationAboutForeignOperationLineItems_01a0582c-fc0a-7eb8-a0b3-4b012219b271" xlink:to="loc_jbsay_LoansandFinancingScheduleofLoansandFinancingDetailsTable_01a0582c-fc0a-7126-9bb8-4ed37854b0b0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0a-79ee-8de8-f989a0e5a071" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_LoansandFinancingScheduleofLoansandFinancingDetailsTable_01a0582c-fc0a-7126-9bb8-4ed37854b0b0" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0a-79ee-8de8-f989a0e5a071" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-79ee-8de8-f989a0e5a071_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0a-79ee-8de8-f989a0e5a071" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-79ee-8de8-f989a0e5a071_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0a-79ee-8de8-f989a0e5a071" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ACCAdvancesOnExchangeContractsMember_01a0582c-fc0a-748b-8322-03b553e352bc" xlink:href="jbsay-20260930.xsd#jbsay_ACCAdvancesOnExchangeContractsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_ACCAdvancesOnExchangeContractsMember_01a0582c-fc0a-748b-8322-03b553e352bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExportCreditNoteMember_01a0582c-fc0a-77c8-94f3-63a890720176" xlink:href="jbsay-20260930.xsd#jbsay_ExportCreditNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_ExportCreditNoteMember_01a0582c-fc0a-77c8-94f3-63a890720176" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WorkingCapital7.28Member_01a1880b-1557-7aaa-94b9-9352c62056b4" xlink:href="jbsay-20260930.xsd#jbsay_WorkingCapital7.28Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_WorkingCapital7.28Member_01a1880b-1557-7aaa-94b9-9352c62056b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WorkingCapital3.63Member_01a1880b-7f82-72c4-8d98-e08a6a078bc6" xlink:href="jbsay-20260930.xsd#jbsay_WorkingCapital3.63Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_WorkingCapital3.63Member_01a1880b-7f82-72c4-8d98-e08a6a078bc6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CRAAgribusinessCreditReceivableCertificatesMember_01a0582c-fc0a-70aa-a267-4dc7fe6acf2e" xlink:href="jbsay-20260930.xsd#jbsay_CRAAgribusinessCreditReceivableCertificatesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_CRAAgribusinessCreditReceivableCertificatesMember_01a0582c-fc0a-70aa-a267-4dc7fe6acf2e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LivestockFinancingMember_01a0582c-fc0a-7f94-8fd9-0851029d0e24" xlink:href="jbsay-20260930.xsd#jbsay_LivestockFinancingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_LivestockFinancingMember_01a0582c-fc0a-7f94-8fd9-0851029d0e24" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PrepaymentMember_01a1880c-d74f-798e-83f2-3c39891f197d" xlink:href="jbsay-20260930.xsd#jbsay_PrepaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_PrepaymentMember_01a1880c-d74f-798e-83f2-3c39891f197d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NotesBondsMember_01a0582c-fc0a-7e89-9994-fc7f1dd04341" xlink:href="jbsay-20260930.xsd#jbsay_NotesBondsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_NotesBondsMember_01a0582c-fc0a-7e89-9994-fc7f1dd04341" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OtherForeignCurrencyMember_01a0582c-fc0a-70b9-848b-f8534483fa4f" xlink:href="jbsay-20260930.xsd#jbsay_OtherForeignCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_OtherForeignCurrencyMember_01a0582c-fc0a-70b9-848b-f8534483fa4f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RevolvingCredit4.01Member_01a18810-0957-74b0-8c19-67968d72c086" xlink:href="jbsay-20260930.xsd#jbsay_RevolvingCredit4.01Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_RevolvingCredit4.01Member_01a18810-0957-74b0-8c19-67968d72c086" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RevolvingCredit5.515.70Member_01a18811-28dd-70f8-9b10-dcb78b04f2a0" xlink:href="jbsay-20260930.xsd#jbsay_RevolvingCredit5.515.70Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_RevolvingCredit5.515.70Member_01a18811-28dd-70f8-9b10-dcb78b04f2a0" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RuralCreditMember_01a18812-4c1e-7881-8c53-c8263abaca23" xlink:href="jbsay-20260930.xsd#jbsay_RuralCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_RuralCreditMember_01a18812-4c1e-7881-8c53-c8263abaca23" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WorkingCapitalEurosMember_01a0582c-fc0a-745a-90db-88cfd40b26a2" xlink:href="jbsay-20260930.xsd#jbsay_WorkingCapitalEurosMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_WorkingCapitalEurosMember_01a0582c-fc0a-745a-90db-88cfd40b26a2" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CDCDirectConsumerCreditMember_01a0582c-fc0a-7e8e-a8b4-ac3489b4915a" xlink:href="jbsay-20260930.xsd#jbsay_CDCDirectConsumerCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_CDCDirectConsumerCreditMember_01a0582c-fc0a-7e8e-a8b4-ac3489b4915a" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DebenturesMember_01a18813-7439-77cd-975a-3ab3379a0f4f" xlink:href="jbsay-20260930.xsd#jbsay_DebenturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_DebenturesMember_01a18813-7439-77cd-975a-3ab3379a0f4f" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OtherLocalCurrencyMember_01a0582c-fc0a-7f49-8782-f9aad5fde0fa" xlink:href="jbsay-20260930.xsd#jbsay_OtherLocalCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0a-73f8-a32b-ea14a577442a" xlink:to="loc_jbsay_OtherLocalCurrencyMember_01a0582c-fc0a-7f49-8782-f9aad5fde0fa" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fc0a-7754-b1c9-c2e1f415a44d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrencyInWhichInformationIsDisplayedAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_LoansandFinancingScheduleofLoansandFinancingDetailsTable_01a0582c-fc0a-7126-9bb8-4ed37854b0b0" xlink:to="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fc0a-7754-b1c9-c2e1f415a44d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fc0a-7754-b1c9-c2e1f415a44d_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FunctionalOrPresentationCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fc0a-7754-b1c9-c2e1f415a44d" xlink:to="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fc0a-7754-b1c9-c2e1f415a44d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fc0a-7120-b995-c00ccddb2245" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FunctionalOrPresentationCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fc0a-7754-b1c9-c2e1f415a44d" xlink:to="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fc0a-7120-b995-c00ccddb2245" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ForeignCurrencyMember_01a0582c-fc0a-735d-9144-4cb98fb1fd08" xlink:href="jbsay-20260930.xsd#jbsay_ForeignCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fc0a-7120-b995-c00ccddb2245" xlink:to="loc_jbsay_ForeignCurrencyMember_01a0582c-fc0a-735d-9144-4cb98fb1fd08" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LocalCurrencyMember_01a0582c-fc0a-723d-9ab3-489093962392" xlink:href="jbsay-20260930.xsd#jbsay_LocalCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fc0a-7120-b995-c00ccddb2245" xlink:to="loc_jbsay_LocalCurrencyMember_01a0582c-fc0a-723d-9ab3-489093962392" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_01a0582c-fc0a-7e00-ac38-0a7c5b338cf0" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_LoansandFinancingScheduleofLoansandFinancingDetailsTable_01a0582c-fc0a-7126-9bb8-4ed37854b0b0" xlink:to="loc_srt_RangeAxis_01a0582c-fc0a-7e00-ac38-0a7c5b338cf0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_01a0582c-fc0a-7e00-ac38-0a7c5b338cf0_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_01a0582c-fc0a-7e00-ac38-0a7c5b338cf0" xlink:to="loc_srt_RangeMember_01a0582c-fc0a-7e00-ac38-0a7c5b338cf0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_01a0582c-fc0a-7102-936f-5c468578512c" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_01a0582c-fc0a-7e00-ac38-0a7c5b338cf0" xlink:to="loc_srt_RangeMember_01a0582c-fc0a-7102-936f-5c468578512c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_01a0582c-fc0a-78f7-b8bf-900c6dfe8744" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_01a0582c-fc0a-7102-936f-5c468578512c" xlink:to="loc_srt_MinimumMember_01a0582c-fc0a-78f7-b8bf-900c6dfe8744" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_01a0582c-fc0a-7409-9edd-73c8a0e9d15e" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_01a0582c-fc0a-7102-936f-5c468578512c" xlink:to="loc_srt_MaximumMember_01a0582c-fc0a-7409-9edd-73c8a0e9d15e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ForeignExchangeRatesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSummarisedFinancialInformationAboutForeignOperationLineItems_01a0582c-fc0a-7eb8-a0b3-4b012219b271" xlink:to="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_BorrowingsInterestRate_01a0582c-fc0a-710f-80bd-8f06263587f9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_BorrowingsInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:to="loc_ifrs-full_BorrowingsInterestRate_01a0582c-fc0a-710f-80bd-8f06263587f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ForeignAndLocalCurrency_01a0582c-fc0a-77a4-b5ad-00bc5f143019" xlink:href="jbsay-20260930.xsd#jbsay_ForeignAndLocalCurrency"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:to="loc_jbsay_ForeignAndLocalCurrency_01a0582c-fc0a-77a4-b5ad-00bc5f143019" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_Indexer_01a0582c-fc0a-73d5-80cd-863485b9bd91" xlink:href="jbsay-20260930.xsd#jbsay_Indexer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:to="loc_jbsay_Indexer_01a0582c-fc0a-73d5-80cd-863485b9bd91" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PaymentTermsnonCurrentDebt_01a0582c-fc0a-77ee-9414-dec207110e8d" xlink:href="jbsay-20260930.xsd#jbsay_PaymentTermsnonCurrentDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:to="loc_jbsay_PaymentTermsnonCurrentDebt_01a0582c-fc0a-77ee-9414-dec207110e8d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FinancingExpensesCurrent_01a0582c-fc0a-7199-b317-b5c2518e6ca7" xlink:href="jbsay-20260930.xsd#jbsay_FinancingExpensesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:to="loc_jbsay_FinancingExpensesCurrent_01a0582c-fc0a-7199-b317-b5c2518e6ca7" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FinancingExpensesNoncurrent_01a0582c-fc0a-724a-b180-575e9e735d89" xlink:href="jbsay-20260930.xsd#jbsay_FinancingExpensesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ForeignExchangeRatesAbstract_01a0582c-fc0a-7658-932d-df3915ca64fe" xlink:to="loc_jbsay_FinancingExpensesNoncurrent_01a0582c-fc0a-724a-b180-575e9e735d89" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/LoansandfinancingNarrativeDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#LoansandfinancingNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/LoansandfinancingNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LoansandFinancingDetailsLineItems_01a0582c-fc0a-73f1-957d-d9ca9dc9d1e5" xlink:href="jbsay-20260930.xsd#jbsay_LoansandFinancingDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LoansandFinancingDetailsTable_01a0582c-fc0a-7f9e-be35-0596373a138b" xlink:href="jbsay-20260930.xsd#jbsay_LoansandFinancingDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_LoansandFinancingDetailsLineItems_01a0582c-fc0a-73f1-957d-d9ca9dc9d1e5" xlink:to="loc_jbsay_LoansandFinancingDetailsTable_01a0582c-fc0a-7f9e-be35-0596373a138b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0a-7c71-a43f-cdb7d4c84720" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_LoansandFinancingDetailsTable_01a0582c-fc0a-7f9e-be35-0596373a138b" xlink:to="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0a-7c71-a43f-cdb7d4c84720" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7c71-a43f-cdb7d4c84720_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0a-7c71-a43f-cdb7d4c84720" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7c71-a43f-cdb7d4c84720_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7ff7-abe3-d7790ccc2fd2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0a-7c71-a43f-cdb7d4c84720" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7ff7-abe3-d7790ccc2fd2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_BR_01a18816-0df6-7fc6-b5af-9a06bbaab2f1" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_BR"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7ff7-abe3-d7790ccc2fd2" xlink:to="loc_country_BR_01a18816-0df6-7fc6-b5af-9a06bbaab2f1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_01a18818-6981-76ec-bb98-eefe729690ef" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7ff7-abe3-d7790ccc2fd2" xlink:to="loc_country_US_01a18818-6981-76ec-bb98-eefe729690ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_NL_01a18816-3883-7b74-87d0-54af8929bf4b" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_NL"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0a-7ff7-abe3-d7790ccc2fd2" xlink:to="loc_country_NL_01a18816-3883-7b74-87d0-54af8929bf4b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RevolvingCreditFacilities_01a0582c-fc0a-7331-8ae5-4253ebf3e9d2" xlink:href="jbsay-20260930.xsd#jbsay_RevolvingCreditFacilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_LoansandFinancingDetailsLineItems_01a0582c-fc0a-73f1-957d-d9ca9dc9d1e5" xlink:to="loc_jbsay_RevolvingCreditFacilities_01a0582c-fc0a-7331-8ae5-4253ebf3e9d2" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/LoansandfinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#LoansandfinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/LoansandfinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScheduleOfNonCurrentPortionOfThePrincipalPaymentOfLoansAndFinancingLineItems_01a0582c-fc0a-7759-9343-b44e8b4607d1" xlink:href="jbsay-20260930.xsd#jbsay_ScheduleOfNonCurrentPortionOfThePrincipalPaymentOfLoansAndFinancingLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LoansandFinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetailsTable_01a0582c-fc0a-7a2a-a056-949f2388499d" xlink:href="jbsay-20260930.xsd#jbsay_LoansandFinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ScheduleOfNonCurrentPortionOfThePrincipalPaymentOfLoansAndFinancingLineItems_01a0582c-fc0a-7759-9343-b44e8b4607d1" xlink:to="loc_jbsay_LoansandFinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetailsTable_01a0582c-fc0a-7a2a-a056-949f2388499d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityAxis_01a0582c-fc0a-7fc9-97e5-bcb4329ebada" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MaturityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_LoansandFinancingScheduleofNonCurrentPortionofthePrincipalPaymentofLoansandFinancingDetailsTable_01a0582c-fc0a-7a2a-a056-949f2388499d" xlink:to="loc_ifrs-full_MaturityAxis_01a0582c-fc0a-7fc9-97e5-bcb4329ebada" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-7fc9-97e5-bcb4329ebada_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MaturityAxis_01a0582c-fc0a-7fc9-97e5-bcb4329ebada" xlink:to="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-7fc9-97e5-bcb4329ebada_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MaturityAxis_01a0582c-fc0a-7fc9-97e5-bcb4329ebada" xlink:to="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanSixMonthsAndNotLaterThanOneYearMember_01a0582c-fc0a-7a98-aa09-c840f67dcdeb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanSixMonthsAndNotLaterThanOneYearMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:to="loc_ifrs-full_LaterThanSixMonthsAndNotLaterThanOneYearMember_01a0582c-fc0a-7a98-aa09-c840f67dcdeb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanOneYearAndNotLaterThanTwoYearsMember_01a0582c-fc0a-7d9c-af09-9af81ea844d6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanOneYearAndNotLaterThanTwoYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:to="loc_ifrs-full_LaterThanOneYearAndNotLaterThanTwoYearsMember_01a0582c-fc0a-7d9c-af09-9af81ea844d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanOneYearAndNotLaterThanThreeYearsMember_01a0582c-fc0a-7126-ae51-208cbf31936f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanOneYearAndNotLaterThanThreeYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:to="loc_ifrs-full_LaterThanOneYearAndNotLaterThanThreeYearsMember_01a0582c-fc0a-7126-ae51-208cbf31936f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanThreeYearsAndNotLaterThanFourYearsMember_01a0582c-fc0a-7e88-8461-e121699ac032" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanThreeYearsAndNotLaterThanFourYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:to="loc_ifrs-full_LaterThanThreeYearsAndNotLaterThanFourYearsMember_01a0582c-fc0a-7e88-8461-e121699ac032" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanOneYearAndNotLaterThanFiveYearsMember_01a0582c-fc0a-75dd-991c-fd41eba181eb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanOneYearAndNotLaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:to="loc_ifrs-full_LaterThanOneYearAndNotLaterThanFiveYearsMember_01a0582c-fc0a-75dd-991c-fd41eba181eb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanFiveYearsMember_01a0582c-fc0a-7c92-875c-b3a1bbb5fa5b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fc0a-714d-80a2-8bd91709b3ab" xlink:to="loc_ifrs-full_LaterThanFiveYearsMember_01a0582c-fc0a-7c92-875c-b3a1bbb5fa5b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LongtermBorrowings_01a0582c-fc0a-76e3-8849-5a906b4ff97e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LongtermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfNonCurrentPortionOfThePrincipalPaymentOfLoansAndFinancingLineItems_01a0582c-fc0a-7759-9343-b44e8b4607d1" xlink:to="loc_ifrs-full_LongtermBorrowings_01a0582c-fc0a-76e3-8849-5a906b4ff97e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/PayrollandsocialchargesScheduleofPayrollandSocialChargesDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#PayrollandsocialchargesScheduleofPayrollandSocialChargesDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/PayrollandsocialchargesScheduleofPayrollandSocialChargesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:href="jbsay-20260930.xsd#jbsay_ScheduleOfPayrollAndSocialChargesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PayrollandSocialChargesScheduleofPayrollandSocialChargesDetailsTable_01a0582c-fc0a-7f8e-94dc-59af6837fbc7" xlink:href="jbsay-20260930.xsd#jbsay_PayrollandSocialChargesScheduleofPayrollandSocialChargesDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_PayrollandSocialChargesScheduleofPayrollandSocialChargesDetailsTable_01a0582c-fc0a-7f8e-94dc-59af6837fbc7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DefinedBenefitPlansAxis_01a0582c-fc0a-75d8-b803-f5a690a966a4" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DefinedBenefitPlansAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_PayrollandSocialChargesScheduleofPayrollandSocialChargesDetailsTable_01a0582c-fc0a-7f8e-94dc-59af6837fbc7" xlink:to="loc_ifrs-full_DefinedBenefitPlansAxis_01a0582c-fc0a-75d8-b803-f5a690a966a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DefinedBenefitPlansDomain_01a0582c-fc0a-75d8-b803-f5a690a966a4_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DefinedBenefitPlansDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_DefinedBenefitPlansAxis_01a0582c-fc0a-75d8-b803-f5a690a966a4" xlink:to="loc_ifrs-full_DefinedBenefitPlansDomain_01a0582c-fc0a-75d8-b803-f5a690a966a4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DefinedBenefitPlansDomain_01a0582c-fc0a-7d9a-82d6-7e29a3542dc5" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DefinedBenefitPlansDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_DefinedBenefitPlansAxis_01a0582c-fc0a-75d8-b803-f5a690a966a4" xlink:to="loc_ifrs-full_DefinedBenefitPlansDomain_01a0582c-fc0a-7d9a-82d6-7e29a3542dc5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PayrollAndSocialChargesMember_01a0582c-fc0a-73a5-b87d-f240df3334d3" xlink:href="jbsay-20260930.xsd#jbsay_PayrollAndSocialChargesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DefinedBenefitPlansDomain_01a0582c-fc0a-7d9a-82d6-7e29a3542dc5" xlink:to="loc_jbsay_PayrollAndSocialChargesMember_01a0582c-fc0a-73a5-b87d-f240df3334d3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SocialChargesInstallments_01a0582c-fc0a-7a30-b956-37698273bc37" xlink:href="jbsay-20260930.xsd#jbsay_SocialChargesInstallments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_SocialChargesInstallments_01a0582c-fc0a-7a30-b956-37698273bc37" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BonusAndVacationAlongWithRelatedSocialCharges_01a0582c-fc0a-7cd3-9d1b-e669010762e1" xlink:href="jbsay-20260930.xsd#jbsay_BonusAndVacationAlongWithRelatedSocialCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_BonusAndVacationAlongWithRelatedSocialCharges_01a0582c-fc0a-7cd3-9d1b-e669010762e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SalariesAndRelatedSocialCharges_01a0582c-fc0a-7140-8575-2c81e9119b83" xlink:href="jbsay-20260930.xsd#jbsay_SalariesAndRelatedSocialCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_SalariesAndRelatedSocialCharges_01a0582c-fc0a-7140-8575-2c81e9119b83" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PayrollAndSocialChargesOthers_01a0582c-fc0a-7e35-8c0d-1a90c9469c4b" xlink:href="jbsay-20260930.xsd#jbsay_PayrollAndSocialChargesOthers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_PayrollAndSocialChargesOthers_01a0582c-fc0a-7e35-8c0d-1a90c9469c4b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PayrollAndSocialCharges_01a0582c-fc0a-77db-a7ed-f15f48305cf5" xlink:href="jbsay-20260930.xsd#jbsay_PayrollAndSocialCharges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_PayrollAndSocialCharges_01a0582c-fc0a-77db-a7ed-f15f48305cf5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_Breakdown1Abstract_01a0582c-fc0a-7686-9406-c520329cd9c1" xlink:href="jbsay-20260930.xsd#jbsay_Breakdown1Abstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfPayrollAndSocialChargesLineItems_01a0582c-fc0a-724c-b33a-63c057230852" xlink:to="loc_jbsay_Breakdown1Abstract_01a0582c-fc0a-7686-9406-c520329cd9c1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentLiabilities_01a0582c-fc0a-7491-bd1d-6b353868d4f2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Breakdown1Abstract_01a0582c-fc0a-7686-9406-c520329cd9c1" xlink:to="loc_ifrs-full_CurrentLiabilities_01a0582c-fc0a-7491-bd1d-6b353868d4f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentLiabilities_01a0582c-fc0a-70cb-85d3-3946942e63a9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Breakdown1Abstract_01a0582c-fc0a-7686-9406-c520329cd9c1" xlink:to="loc_ifrs-full_NoncurrentLiabilities_01a0582c-fc0a-70cb-85d3-3946942e63a9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Liabilities_01a0582c-fc0a-732d-9c82-de71cf053f74" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Liabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_Breakdown1Abstract_01a0582c-fc0a-7686-9406-c520329cd9c1" xlink:to="loc_ifrs-full_Liabilities_01a0582c-fc0a-732d-9c82-de71cf053f74" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/ProvisionsforlegalproceedingsScheduleofBreakdownLiabilitiesDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#ProvisionsforlegalproceedingsScheduleofBreakdownLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/ProvisionsforlegalproceedingsScheduleofBreakdownLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsLineItems_01a0582c-fc0b-7b55-a2c5-ee045303ee08" xlink:href="jbsay-20260930.xsd#jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsTable_01a0582c-fc0b-7512-8fa8-4fa89155dbfd" xlink:href="jbsay-20260930.xsd#jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsLineItems_01a0582c-fc0b-7b55-a2c5-ee045303ee08" xlink:to="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsTable_01a0582c-fc0b-7512-8fa8-4fa89155dbfd" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesAxis_01a0582c-fc0b-71fe-bec4-a4142edff563" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsTable_01a0582c-fc0b-7512-8fa8-4fa89155dbfd" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesAxis_01a0582c-fc0b-71fe-bec4-a4142edff563" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesDomain_01a0582c-fc0b-71fe-bec4-a4142edff563_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesAxis_01a0582c-fc0b-71fe-bec4-a4142edff563" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesDomain_01a0582c-fc0b-71fe-bec4-a4142edff563_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfLiabilitiesDomain_01a0582c-fc0b-7d67-b0ca-f3f56dd0d60c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesAxis_01a0582c-fc0b-71fe-bec4-a4142edff563" xlink:to="loc_ifrs-full_ClassesOfLiabilitiesDomain_01a0582c-fc0b-7d67-b0ca-f3f56dd0d60c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BreakdownLiabilitiesMember_01a0582c-fc0b-751f-988c-99767af753c5" xlink:href="jbsay-20260930.xsd#jbsay_BreakdownLiabilitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfLiabilitiesDomain_01a0582c-fc0b-7d67-b0ca-f3f56dd0d60c" xlink:to="loc_jbsay_BreakdownLiabilitiesMember_01a0582c-fc0b-751f-988c-99767af753c5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherShorttermProvisions_01a0582c-fc0b-7780-891f-2e794b5fd98c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherShorttermProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsLineItems_01a0582c-fc0b-7b55-a2c5-ee045303ee08" xlink:to="loc_ifrs-full_OtherShorttermProvisions_01a0582c-fc0b-7780-891f-2e794b5fd98c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherLongtermProvisions_01a0582c-fc0b-71ee-8ac2-1f9dc262bc1d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherLongtermProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsLineItems_01a0582c-fc0b-7b55-a2c5-ee045303ee08" xlink:to="loc_ifrs-full_OtherLongtermProvisions_01a0582c-fc0b-71ee-8ac2-1f9dc262bc1d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherProvisions_01a0582c-fc0b-7496-9c79-f56815727569" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofBreakdownLiabilitiesDetailsLineItems_01a0582c-fc0b-7b55-a2c5-ee045303ee08" xlink:to="loc_ifrs-full_OtherProvisions_01a0582c-fc0b-7496-9c79-f56815727569" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/ProvisionsforlegalproceedingsScheduleofChangesinProvisionsDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#ProvisionsforlegalproceedingsScheduleofChangesinProvisionsDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/ProvisionsforlegalproceedingsScheduleofChangesinProvisionsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfOtherProvisionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ProvisionsforLegalProceedingsScheduleofChangesinProvisionsDetailsTable_01a0582c-fc0b-7a31-9671-7bc55386febe" xlink:href="jbsay-20260930.xsd#jbsay_ProvisionsforLegalProceedingsScheduleofChangesinProvisionsDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_jbsay_ProvisionsforLegalProceedingsScheduleofChangesinProvisionsDetailsTable_01a0582c-fc0b-7a31-9671-7bc55386febe" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7409-b9cc-97cc62290efc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofChangesinProvisionsDetailsTable_01a0582c-fc0b-7a31-9671-7bc55386febe" xlink:to="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7409-b9cc-97cc62290efc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7409-b9cc-97cc62290efc_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7409-b9cc-97cc62290efc" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7409-b9cc-97cc62290efc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7eba-be7d-f2b699b31765" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7409-b9cc-97cc62290efc" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7eba-be7d-f2b699b31765" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_BR_01a0582c-fc0b-7879-b989-a9a76f114643" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_BR"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7eba-be7d-f2b699b31765" xlink:to="loc_country_BR_01a0582c-fc0b-7879-b989-a9a76f114643" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_01a0582c-fc0b-71d6-be82-479b156f7bc9" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7eba-be7d-f2b699b31765" xlink:to="loc_country_US_01a0582c-fc0b-71d6-be82-479b156f7bc9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OthersJurisdictionsMember_01a0582c-fc0b-773d-a3d5-81da96d25a46" xlink:href="jbsay-20260930.xsd#jbsay_OthersJurisdictionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7eba-be7d-f2b699b31765" xlink:to="loc_jbsay_OthersJurisdictionsMember_01a0582c-fc0b-773d-a3d5-81da96d25a46" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfProvisionsAxis_01a0582c-fc0b-76d2-904b-7f80c698f7ee" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfProvisionsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_ProvisionsforLegalProceedingsScheduleofChangesinProvisionsDetailsTable_01a0582c-fc0b-7a31-9671-7bc55386febe" xlink:to="loc_ifrs-full_ClassesOfProvisionsAxis_01a0582c-fc0b-76d2-904b-7f80c698f7ee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-76d2-904b-7f80c698f7ee_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfOtherProvisionsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfProvisionsAxis_01a0582c-fc0b-76d2-904b-7f80c698f7ee" xlink:to="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-76d2-904b-7f80c698f7ee_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-7c98-8542-21b2c3b0cbf3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfOtherProvisionsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfProvisionsAxis_01a0582c-fc0b-76d2-904b-7f80c698f7ee" xlink:to="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-7c98-8542-21b2c3b0cbf3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LaborChangesInProvisionsMember_01a0582c-fc0b-7a47-9a8c-8c413f188f74" xlink:href="jbsay-20260930.xsd#jbsay_LaborChangesInProvisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-7c98-8542-21b2c3b0cbf3" xlink:to="loc_jbsay_LaborChangesInProvisionsMember_01a0582c-fc0b-7a47-9a8c-8c413f188f74" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CivilChangesInProvisionsMember_01a0582c-fc0b-7eaa-a8e1-8a95bdcc3055" xlink:href="jbsay-20260930.xsd#jbsay_CivilChangesInProvisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-7c98-8542-21b2c3b0cbf3" xlink:to="loc_jbsay_CivilChangesInProvisionsMember_01a0582c-fc0b-7eaa-a8e1-8a95bdcc3055" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TaxAndSocialSecurityChangesInProvisionMember_01a0582c-fc0b-74f6-b2b7-d7f0cb5005c4" xlink:href="jbsay-20260930.xsd#jbsay_TaxAndSocialSecurityChangesInProvisionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfOtherProvisionsDomain_01a0582c-fc0b-7c98-8542-21b2c3b0cbf3" xlink:to="loc_jbsay_TaxAndSocialSecurityChangesInProvisionMember_01a0582c-fc0b-74f6-b2b7-d7f0cb5005c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherProvisions_01a0582c-fc0b-7733-93e0-395c5824c895" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_ifrs-full_OtherProvisions_01a0582c-fc0b-7733-93e0-395c5824c895" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdditionalProvisionsOtherProvisions_01a0582c-fc0b-7031-9dad-62056592f406" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdditionalProvisionsOtherProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_ifrs-full_AdditionalProvisionsOtherProvisions_01a0582c-fc0b-7031-9dad-62056592f406" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdditionalProvisionsOtherProvisionsAdjusted_01a0582c-fc0b-7605-a26e-dd49b682e320" xlink:href="jbsay-20260930.xsd#jbsay_AdditionalProvisionsOtherProvisionsAdjusted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_jbsay_AdditionalProvisionsOtherProvisionsAdjusted_01a0582c-fc0b-7605-a26e-dd49b682e320" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesOtherProvisions_01a0582c-fc0b-7256-9f51-5d08283c83cd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesOtherProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughTransfersAndOtherChangesOtherProvisions_01a0582c-fc0b-7256-9f51-5d08283c83cd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IncreaseDecreaseInExistingProvisionsOtherProvisions1_01a0582c-fc0b-766a-819a-8f5bc13e46f3" xlink:href="jbsay-20260930.xsd#jbsay_IncreaseDecreaseInExistingProvisionsOtherProvisions1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_jbsay_IncreaseDecreaseInExistingProvisionsOtherProvisions1_01a0582c-fc0b-766a-819a-8f5bc13e46f3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesOtherProvisions_01a0582c-fc0b-7cf1-a4df-b5eab21d22ee" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesOtherProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOtherProvisionsLineItems_01a0582c-fc0b-7ce3-831e-ceb7b3aa9775" xlink:to="loc_ifrs-full_IncreaseDecreaseThroughNetExchangeDifferencesOtherProvisions_01a0582c-fc0b-7cf1-a4df-b5eab21d22ee" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherProvisions_01a0582c-fc0b-751b-b506-2cf9dda4740e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherProvisions"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/NetrevenueScheduleofRevenuebySignificantCategoryScheduleofNetRevenueDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#NetrevenueScheduleofRevenuebySignificantCategoryScheduleofNetRevenueDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/NetrevenueScheduleofRevenuebySignificantCategoryScheduleofNetRevenueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScheduleOfRevenueBySignificantCategoryLineItems_01a0582c-fc0b-7db1-a8d0-e0924d8ebb62" xlink:href="jbsay-20260930.xsd#jbsay_ScheduleOfRevenueBySignificantCategoryLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetRevenueScheduleofRevenuebySignificantCategoryDetailsTable_01a0582c-fc0b-7df9-a2fa-a27236f73599" xlink:href="jbsay-20260930.xsd#jbsay_NetRevenueScheduleofRevenuebySignificantCategoryDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ScheduleOfRevenueBySignificantCategoryLineItems_01a0582c-fc0b-7db1-a8d0-e0924d8ebb62" xlink:to="loc_jbsay_NetRevenueScheduleofRevenuebySignificantCategoryDetailsTable_01a0582c-fc0b-7df9-a2fa-a27236f73599" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesChannelsAxis_01a0582c-fc0b-7a4e-a277-c516d0dc4e52" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SalesChannelsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_NetRevenueScheduleofRevenuebySignificantCategoryDetailsTable_01a0582c-fc0b-7df9-a2fa-a27236f73599" xlink:to="loc_ifrs-full_SalesChannelsAxis_01a0582c-fc0b-7a4e-a277-c516d0dc4e52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesChannelsDomain_01a0582c-fc0b-7a4e-a277-c516d0dc4e52_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SalesChannelsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SalesChannelsAxis_01a0582c-fc0b-7a4e-a277-c516d0dc4e52" xlink:to="loc_ifrs-full_SalesChannelsDomain_01a0582c-fc0b-7a4e-a277-c516d0dc4e52_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SalesChannelsDomain_01a0582c-fc0b-7a54-ba8a-60d8ba610aff" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SalesChannelsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SalesChannelsAxis_01a0582c-fc0b-7a4e-a277-c516d0dc4e52" xlink:to="loc_ifrs-full_SalesChannelsDomain_01a0582c-fc0b-7a54-ba8a-60d8ba610aff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DomesticSalesMember_01a0582c-fc0b-7006-88a4-842735184a0d" xlink:href="jbsay-20260930.xsd#jbsay_DomesticSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SalesChannelsDomain_01a0582c-fc0b-7a54-ba8a-60d8ba610aff" xlink:to="loc_jbsay_DomesticSalesMember_01a0582c-fc0b-7006-88a4-842735184a0d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExportSalesMember_01a0582c-fc0b-7baf-9f48-3bb15e561aad" xlink:href="jbsay-20260930.xsd#jbsay_ExportSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SalesChannelsDomain_01a0582c-fc0b-7a54-ba8a-60d8ba610aff" xlink:to="loc_jbsay_ExportSalesMember_01a0582c-fc0b-7baf-9f48-3bb15e561aad" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Revenue_01a0582c-fc0b-7406-97f4-0c2564de968e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Revenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfRevenueBySignificantCategoryLineItems_01a0582c-fc0b-7db1-a8d0-e0924d8ebb62" xlink:to="loc_ifrs-full_Revenue_01a0582c-fc0b-7406-97f4-0c2564de968e" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/OperatingsegmentsScheduleofOperatingSegmentDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#OperatingsegmentsScheduleofOperatingSegmentDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/OperatingsegmentsScheduleofOperatingSegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfOperatingSegmentsLineItems_01a0582c-fc0b-76fb-8580-7f80f689272e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfOperatingSegmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingSegmentsScheduleofOperatingSegmentDetailsTable_01a0582c-fc0b-7a4b-a0e1-2ed0d5b74000" xlink:href="jbsay-20260930.xsd#jbsay_OperatingSegmentsScheduleofOperatingSegmentDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfOperatingSegmentsLineItems_01a0582c-fc0b-76fb-8580-7f80f689272e" xlink:to="loc_jbsay_OperatingSegmentsScheduleofOperatingSegmentDetailsTable_01a0582c-fc0b-7a4b-a0e1-2ed0d5b74000" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-76e6-96b4-73d4eb23c522" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_OperatingSegmentsScheduleofOperatingSegmentDetailsTable_01a0582c-fc0b-7a4b-a0e1-2ed0d5b74000" xlink:to="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-76e6-96b4-73d4eb23c522" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-76e6-96b4-73d4eb23c522_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-76e6-96b4-73d4eb23c522" xlink:to="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-76e6-96b4-73d4eb23c522_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7cbc-a900-f89c2631c4b9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-76e6-96b4-73d4eb23c522" xlink:to="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7cbc-a900-f89c2631c4b9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ReportableSegmentsMember_01a0582c-fc0b-747f-8133-c95d6f070e66" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ReportableSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7cbc-a900-f89c2631c4b9" xlink:to="loc_ifrs-full_ReportableSegmentsMember_01a0582c-fc0b-747f-8133-c95d6f070e66" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AllOtherSegmentsMember_01a0582c-fc0b-728a-833f-1377e35acfa3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AllOtherSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7cbc-a900-f89c2631c4b9" xlink:to="loc_ifrs-full_AllOtherSegmentsMember_01a0582c-fc0b-728a-833f-1377e35acfa3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-71f1-a00e-2f4545fd9428" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_OperatingSegmentsScheduleofOperatingSegmentDetailsTable_01a0582c-fc0b-7a4b-a0e1-2ed0d5b74000" xlink:to="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-71f1-a00e-2f4545fd9428" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-71f1-a00e-2f4545fd9428_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-71f1-a00e-2f4545fd9428" xlink:to="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-71f1-a00e-2f4545fd9428_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c80-b8a6-1c478af7c5bd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-71f1-a00e-2f4545fd9428" xlink:to="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c80-b8a6-1c478af7c5bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OperatingSegmentsMember_01a0582c-fc0b-7d02-ab60-3fa261f3076a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c80-b8a6-1c478af7c5bd" xlink:to="loc_ifrs-full_OperatingSegmentsMember_01a0582c-fc0b-7d02-ab60-3fa261f3076a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_UnallocatedAmountsMember_01a0582c-fc0b-715c-a2d8-5c5a9e862374" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_UnallocatedAmountsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c80-b8a6-1c478af7c5bd" xlink:to="loc_ifrs-full_UnallocatedAmountsMember_01a0582c-fc0b-715c-a2d8-5c5a9e862374" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EliminationOfIntersegmentAmountsMember_01a0582c-fc0b-7af9-a263-5bac30de0c7c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EliminationOfIntersegmentAmountsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c80-b8a6-1c478af7c5bd" xlink:to="loc_ifrs-full_EliminationOfIntersegmentAmountsMember_01a0582c-fc0b-7af9-a263-5bac30de0c7c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProductsAndServicesAxis_01a0582c-fc0b-7405-8425-3a1bb91d407d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProductsAndServicesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_OperatingSegmentsScheduleofOperatingSegmentDetailsTable_01a0582c-fc0b-7a4b-a0e1-2ed0d5b74000" xlink:to="loc_ifrs-full_ProductsAndServicesAxis_01a0582c-fc0b-7405-8425-3a1bb91d407d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7405-8425-3a1bb91d407d_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ProductsAndServicesAxis_01a0582c-fc0b-7405-8425-3a1bb91d407d" xlink:to="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7405-8425-3a1bb91d407d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ProductsAndServicesAxis_01a0582c-fc0b-7405-8425-3a1bb91d407d" xlink:to="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BrazilSegmentMember_01a0582c-fc0b-79bd-af40-8bbc138de286" xlink:href="jbsay-20260930.xsd#jbsay_BrazilSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:to="loc_jbsay_BrazilSegmentMember_01a0582c-fc0b-79bd-af40-8bbc138de286" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SearaMember_01a0582c-fc0b-7b23-8841-389e5eaed912" xlink:href="jbsay-20260930.xsd#jbsay_SearaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:to="loc_jbsay_SearaMember_01a0582c-fc0b-7b23-8841-389e5eaed912" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_BeefNorthAmericaMember_01a0582c-fc0b-7b97-a33f-3d9eb8534e3f" xlink:href="jbsay-20260930.xsd#jbsay_BeefNorthAmericaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:to="loc_jbsay_BeefNorthAmericaMember_01a0582c-fc0b-7b97-a33f-3d9eb8534e3f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_USAPorkMember_01a0582c-fc0b-76ab-bf35-e3341bb57e5e" xlink:href="jbsay-20260930.xsd#jbsay_USAPorkMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:to="loc_jbsay_USAPorkMember_01a0582c-fc0b-76ab-bf35-e3341bb57e5e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PilgrimsPrideMember_01a0582c-fc0b-713f-b181-7ac77b3397bb" xlink:href="jbsay-20260930.xsd#jbsay_PilgrimsPrideMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:to="loc_jbsay_PilgrimsPrideMember_01a0582c-fc0b-713f-b181-7ac77b3397bb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AustraliaSegmentMember_01a0582c-fc0b-74c8-bec7-d2f2e0019140" xlink:href="jbsay-20260930.xsd#jbsay_AustraliaSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProductsAndServicesDomain_01a0582c-fc0b-7559-8c8b-8e8e4b2a261d" xlink:to="loc_jbsay_AustraliaSegmentMember_01a0582c-fc0b-74c8-bec7-d2f2e0019140" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RevenueFromExternalCustomers_01a0582c-fc0b-7e90-b3fa-aa00c6c0539e" xlink:href="jbsay-20260930.xsd#jbsay_RevenueFromExternalCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOperatingSegmentsLineItems_01a0582c-fc0b-76fb-8580-7f80f689272e" xlink:to="loc_jbsay_RevenueFromExternalCustomers_01a0582c-fc0b-7e90-b3fa-aa00c6c0539e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IntersegmentRevenue_01a0582c-fc0b-7605-b48e-db584aa8171f" xlink:href="jbsay-20260930.xsd#jbsay_IntersegmentRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOperatingSegmentsLineItems_01a0582c-fc0b-76fb-8580-7f80f689272e" xlink:to="loc_jbsay_IntersegmentRevenue_01a0582c-fc0b-7605-b48e-db584aa8171f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Revenue_01a0582c-fc0b-76f3-afbc-3479c0f1408d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Revenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOperatingSegmentsLineItems_01a0582c-fc0b-76fb-8580-7f80f689272e" xlink:to="loc_ifrs-full_Revenue_01a0582c-fc0b-76f3-afbc-3479c0f1408d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AdjustedEBITDA_01a0582c-fc0b-7551-9ffe-6857a5608040" xlink:href="jbsay-20260930.xsd#jbsay_AdjustedEBITDA"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfOperatingSegmentsLineItems_01a0582c-fc0b-76fb-8580-7f80f689272e" xlink:to="loc_jbsay_AdjustedEBITDA_01a0582c-fc0b-7551-9ffe-6857a5608040" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/OperatingsegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#OperatingsegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/OperatingsegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfGeographicalAreasLineItems_01a0582c-fc0b-74ab-9b76-871f95b9816f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfGeographicalAreasLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingSegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetailsTable_01a0582c-fc0b-7aa6-8693-1e28d3458b3e" xlink:href="jbsay-20260930.xsd#jbsay_OperatingSegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfGeographicalAreasLineItems_01a0582c-fc0b-74ab-9b76-871f95b9816f" xlink:to="loc_jbsay_OperatingSegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetailsTable_01a0582c-fc0b-7aa6-8693-1e28d3458b3e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-70a4-b927-d2ea7cc95490" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_OperatingSegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetailsTable_01a0582c-fc0b-7aa6-8693-1e28d3458b3e" xlink:to="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-70a4-b927-d2ea7cc95490" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-70a4-b927-d2ea7cc95490_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-70a4-b927-d2ea7cc95490" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-70a4-b927-d2ea7cc95490_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-70a4-b927-d2ea7cc95490" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_01a0582c-fc0b-775b-99fe-db3730844aa1" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:to="loc_country_US_01a0582c-fc0b-775b-99fe-db3730844aa1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MexicoAndCanadaMember_01a0582c-fc0b-79d2-822a-8f4f648683a2" xlink:href="jbsay-20260930.xsd#jbsay_MexicoAndCanadaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:to="loc_jbsay_MexicoAndCanadaMember_01a0582c-fc0b-79d2-822a-8f4f648683a2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_BR_01a0582c-fc0b-73dc-b2d0-03b3151aa8e1" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_BR"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:to="loc_country_BR_01a0582c-fc0b-73dc-b2d0-03b3151aa8e1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_AU_01a0582c-fc0b-7d50-998a-f7d8b61041a1" xlink:href="https://xbrl.sec.gov/country/2025/country-2025.xsd#country_AU"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:to="loc_country_AU_01a0582c-fc0b-7d50-998a-f7d8b61041a1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_EuropeMember_01a0582c-fc0b-73b4-ad91-8b5e4ba2c575" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_EuropeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:to="loc_srt_EuropeMember_01a0582c-fc0b-73b4-ad91-8b5e4ba2c575" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MinorRegionsMember_01a0582c-fc0b-7935-88bb-40615b91f003" xlink:href="jbsay-20260930.xsd#jbsay_MinorRegionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-727c-9cfc-bb9595ac6948" xlink:to="loc_jbsay_MinorRegionsMember_01a0582c-fc0b-7935-88bb-40615b91f003" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-7c74-971f-8af1fc73cd95" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_OperatingSegmentsScheduleofNetRevenueandTotalAssetsBasedonGeographyDetailsTable_01a0582c-fc0b-7aa6-8693-1e28d3458b3e" xlink:to="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-7c74-971f-8af1fc73cd95" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c74-971f-8af1fc73cd95_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-7c74-971f-8af1fc73cd95" xlink:to="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-7c74-971f-8af1fc73cd95_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-74b3-8c44-d8764b3b7f0b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SegmentConsolidationItemsAxis_01a0582c-fc0b-7c74-971f-8af1fc73cd95" xlink:to="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-74b3-8c44-d8764b3b7f0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OperatingSegmentsMember_01a0582c-fc0b-778d-8824-b36b1e61304c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OperatingSegmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-74b3-8c44-d8764b3b7f0b" xlink:to="loc_ifrs-full_OperatingSegmentsMember_01a0582c-fc0b-778d-8824-b36b1e61304c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EliminationOfIntersegmentAmountsMember_01a0582c-fc0b-7cfc-8333-07c72baa07f1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EliminationOfIntersegmentAmountsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentConsolidationItemsDomain_01a0582c-fc0b-74b3-8c44-d8764b3b7f0b" xlink:to="loc_ifrs-full_EliminationOfIntersegmentAmountsMember_01a0582c-fc0b-7cfc-8333-07c72baa07f1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherRevenue_01a0582c-fc0b-7714-964f-88b0c1c4070a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfGeographicalAreasLineItems_01a0582c-fc0b-74ab-9b76-871f95b9816f" xlink:to="loc_ifrs-full_OtherRevenue_01a0582c-fc0b-7714-964f-88b0c1c4070a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherAssets_01a0582c-fc0b-738f-a4e6-579319493b50" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfGeographicalAreasLineItems_01a0582c-fc0b-74ab-9b76-871f95b9816f" xlink:to="loc_ifrs-full_OtherAssets_01a0582c-fc0b-738f-a4e6-579319493b50" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/ExpensesbynatureScheduleofExpensesbyNatureDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#ExpensesbynatureScheduleofExpensesbyNatureDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/ExpensesbynatureScheduleofExpensesbyNatureDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfAttributionOfExpensesByNatureToTheirFunctionLineItems_01a0582c-fc0b-7bea-a95a-c956429af622" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfAttributionOfExpensesByNatureToTheirFunctionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExpensesbyNatureScheduleofExpensesbyNatureDetailsTable_01a0582c-fc0b-7ac3-98bd-0b2c1033ca54" xlink:href="jbsay-20260930.xsd#jbsay_ExpensesbyNatureScheduleofExpensesbyNatureDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfAttributionOfExpensesByNatureToTheirFunctionLineItems_01a0582c-fc0b-7bea-a95a-c956429af622" xlink:to="loc_jbsay_ExpensesbyNatureScheduleofExpensesbyNatureDetailsTable_01a0582c-fc0b-7ac3-98bd-0b2c1033ca54" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-77df-ad24-d0c84df3b742" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_ExpensesbyNatureScheduleofExpensesbyNatureDetailsTable_01a0582c-fc0b-7ac3-98bd-0b2c1033ca54" xlink:to="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-77df-ad24-d0c84df3b742" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-77df-ad24-d0c84df3b742_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-77df-ad24-d0c84df3b742" xlink:to="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-77df-ad24-d0c84df3b742_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7458-af6c-0adbf0f56fd0" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SegmentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_SegmentsAxis_01a0582c-fc0b-77df-ad24-d0c84df3b742" xlink:to="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7458-af6c-0adbf0f56fd0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_StatementOfIncomeMember_01a0582c-fc0b-70c9-9223-fe7871c67eb7" xlink:href="jbsay-20260930.xsd#jbsay_StatementOfIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_SegmentsDomain_01a0582c-fc0b-7458-af6c-0adbf0f56fd0" xlink:to="loc_jbsay_StatementOfIncomeMember_01a0582c-fc0b-70c9-9223-fe7871c67eb7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_01a0582c-fc0b-75f1-bf05-219d9cb73cd3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_ExpensesbyNatureScheduleofExpensesbyNatureDetailsTable_01a0582c-fc0b-7ac3-98bd-0b2c1033ca54" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_01a0582c-fc0b-75f1-bf05-219d9cb73cd3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-75f1-bf05-219d9cb73cd3_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_01a0582c-fc0b-75f1-bf05-219d9cb73cd3" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-75f1-bf05-219d9cb73cd3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-7bef-9bf4-60aa3f7987cd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionAxis_01a0582c-fc0b-75f1-bf05-219d9cb73cd3" xlink:to="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-7bef-9bf4-60aa3f7987cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfSalesMember_01a0582c-fc0b-78a1-ab7c-bda0b40e3d2b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-7bef-9bf4-60aa3f7987cd" xlink:to="loc_ifrs-full_CostOfSalesMember_01a0582c-fc0b-78a1-ab7c-bda0b40e3d2b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SellingMember_01a0582c-fc0b-7aeb-b846-0deaf12cd5e0" xlink:href="jbsay-20260930.xsd#jbsay_SellingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-7bef-9bf4-60aa3f7987cd" xlink:to="loc_jbsay_SellingMember_01a0582c-fc0b-7aeb-b846-0deaf12cd5e0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SellingGeneralAndAdministrativeExpenseMember_01a0582c-fc0b-743e-90d9-dc0de0194143" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SellingGeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_AttributionOfExpensesByNatureToTheirFunctionDomain_01a0582c-fc0b-7bef-9bf4-60aa3f7987cd" xlink:to="loc_ifrs-full_SellingGeneralAndAdministrativeExpenseMember_01a0582c-fc0b-743e-90d9-dc0de0194143" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CostOfHedgingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfAttributionOfExpensesByNatureToTheirFunctionLineItems_01a0582c-fc0b-7bea-a95a-c956429af622" xlink:to="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RawMaterialsAndConsumablesUsed_01a0582c-fc0b-7db1-a432-b76dfe29fb9b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RawMaterialsAndConsumablesUsed"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_RawMaterialsAndConsumablesUsed_01a0582c-fc0b-7db1-a432-b76dfe29fb9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_WagesAndSalaries_01a0582c-fc0b-7beb-a752-9e1768bd6e77" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WagesAndSalaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_WagesAndSalaries_01a0582c-fc0b-7beb-a752-9e1768bd6e77" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DepreciationAndAmortisationExpense_01a0582c-fc0b-7d84-bc26-bfa41f7b4c87" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DepreciationAndAmortisationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_DepreciationAndAmortisationExpense_01a0582c-fc0b-7d84-bc26-bfa41f7b4c87" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfSales_01a0582c-fc0b-708b-ab4b-0a49a397d179" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CostOfSales"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_CostOfSales_01a0582c-fc0b-708b-ab4b-0a49a397d179" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FreightAndSellingExpenses_01a0582c-fc0b-750a-bc78-c22f8732e88f" xlink:href="jbsay-20260930.xsd#jbsay_FreightAndSellingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_jbsay_FreightAndSellingExpenses_01a0582c-fc0b-750a-bc78-c22f8732e88f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_AdvertisingExpense_01a0582c-fc0b-7472-aed0-23f74438637d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AdvertisingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_AdvertisingExpense_01a0582c-fc0b-7472-aed0-23f74438637d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FeeAndCommissionExpense_01a0582c-fc0b-7013-a92a-902476a3aa47" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FeeAndCommissionExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_FeeAndCommissionExpense_01a0582c-fc0b-7013-a92a-902476a3aa47" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ImpairmentLoss_01a0582c-fc0b-70fc-b490-7a5d963415ee" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_ImpairmentLoss_01a0582c-fc0b-70fc-b490-7a5d963415ee" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SellingExpense_01a0582c-fc0b-7658-a6ca-bd812944aec1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SellingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_SellingExpense_01a0582c-fc0b-7658-a6ca-bd812944aec1" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FeesServicesPurchasedAndGeneralExpenses_01a0582c-fc0b-7698-a5a6-8a2eb1f913c5" xlink:href="jbsay-20260930.xsd#jbsay_FeesServicesPurchasedAndGeneralExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_jbsay_FeesServicesPurchasedAndGeneralExpenses_01a0582c-fc0b-7698-a5a6-8a2eb1f913c5" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DOJDepartmentOfJusticeAndAntitrustAgreements_01a0582c-fc0b-73ba-84f6-688c7d31a6c3" xlink:href="jbsay-20260930.xsd#jbsay_DOJDepartmentOfJusticeAndAntitrustAgreements"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_jbsay_DOJDepartmentOfJusticeAndAntitrustAgreements_01a0582c-fc0b-73ba-84f6-688c7d31a6c3" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DonationsAndSubsidiesExpense_01a0582c-fc0b-7f71-aacc-d24503b2f8c2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DonationsAndSubsidiesExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_DonationsAndSubsidiesExpense_01a0582c-fc0b-7f71-aacc-d24503b2f8c2" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeneralAndAdministrativeExpense_01a0582c-fc0b-70bc-8917-73a8a7a2c092" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CostOfHedgingAbstract_01a0582c-fc0b-7fb4-9c9a-38637d57d8ae" xlink:to="loc_ifrs-full_GeneralAndAdministrativeExpense_01a0582c-fc0b-70bc-8917-73a8a7a2c092" xlink:type="arc" order="13"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems_01a0582c-fc0b-74f2-a0aa-710ef54a7018" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsTable_01a0582c-fc0b-7d83-a59d-c9f1d7f6a893" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems_01a0582c-fc0b-74f2-a0aa-710ef54a7018" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsTable_01a0582c-fc0b-7d83-a59d-c9f1d7f6a893" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0b-7e28-ad69-41559d7b7223" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsTable_01a0582c-fc0b-7d83-a59d-c9f1d7f6a893" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0b-7e28-ad69-41559d7b7223" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7e28-ad69-41559d7b7223_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0b-7e28-ad69-41559d7b7223" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7e28-ad69-41559d7b7223_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fc0b-7e28-ad69-41559d7b7223" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CRAAgribusinessCreditReceivableCertificatesMember_01a0582c-fc0b-75e1-a37b-ff76778ab33d" xlink:href="jbsay-20260930.xsd#jbsay_CRAAgribusinessCreditReceivableCertificatesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_CRAAgribusinessCreditReceivableCertificatesMember_01a0582c-fc0b-75e1-a37b-ff76778ab33d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CreditNoteExportMember_01a0582c-fc0b-7296-838e-7d054aeb116d" xlink:href="jbsay-20260930.xsd#jbsay_CreditNoteExportMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_CreditNoteExportMember_01a0582c-fc0b-7296-838e-7d054aeb116d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RuralCreditNotePrefixedMember_01a0582c-fc0b-7bf0-8827-7d8036538ba5" xlink:href="jbsay-20260930.xsd#jbsay_RuralCreditNotePrefixedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_RuralCreditNotePrefixedMember_01a0582c-fc0b-7bf0-8827-7d8036538ba5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RelatedPartyTransactionsMember_01a0582c-fc0b-7365-8d7f-8265c87ce83d" xlink:href="jbsay-20260930.xsd#jbsay_RelatedPartyTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_RelatedPartyTransactionsMember_01a0582c-fc0b-7365-8d7f-8265c87ce83d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CDBDIBankCertificatesOfDepositMember_01a0582c-fc0b-73ab-9729-26761c4f99e2" xlink:href="jbsay-20260930.xsd#jbsay_CDBDIBankCertificatesOfDepositMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_CDBDIBankCertificatesOfDepositMember_01a0582c-fc0b-73ab-9729-26761c4f99e2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_MarginCashMember_01a0582c-fc0b-72ab-8734-0692e82fbf76" xlink:href="jbsay-20260930.xsd#jbsay_MarginCashMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_MarginCashMember_01a0582c-fc0b-72ab-8734-0692e82fbf76" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TreasuryBillsMember_01a0582c-fc0b-7383-918e-132d9b881c35" xlink:href="jbsay-20260930.xsd#jbsay_TreasuryBillsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_TreasuryBillsMember_01a0582c-fc0b-7383-918e-132d9b881c35" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DebenturesMember_01a1881d-f12a-7669-818e-edbf0de62a58" xlink:href="jbsay-20260930.xsd#jbsay_DebenturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_DebenturesMember_01a1881d-f12a-7669-818e-edbf0de62a58" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SubtotalMember_01a0582c-fc0b-7b8f-a911-50b878d7603c" xlink:href="jbsay-20260930.xsd#jbsay_SubtotalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_SubtotalMember_01a0582c-fc0b-7b8f-a911-50b878d7603c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DerivativesSwapMember_01a0582c-fc0b-7403-ad93-48ec0e7856bc" xlink:href="jbsay-20260930.xsd#jbsay_DerivativesSwapMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_DerivativesSwapMember_01a0582c-fc0b-7403-ad93-48ec0e7856bc" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExportCreditNoteMember_01a0582c-fc0b-75e3-b6bc-7a8e9fb104b2" xlink:href="jbsay-20260930.xsd#jbsay_ExportCreditNoteMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_ExportCreditNoteMember_01a0582c-fc0b-75e3-b6bc-7a8e9fb104b2" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PrepaymentExchangeAgreementMember_01a18820-ecf2-7899-b30e-a71462e853fc" xlink:href="jbsay-20260930.xsd#jbsay_PrepaymentExchangeAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_PrepaymentExchangeAgreementMember_01a18820-ecf2-7899-b30e-a71462e853fc" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_WorkingCapitalMember_01a0582c-fc0b-75f4-916c-aaea2fa5f1a1" xlink:href="jbsay-20260930.xsd#jbsay_WorkingCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_WorkingCapitalMember_01a0582c-fc0b-75f4-916c-aaea2fa5f1a1" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RevolvingCreditFacility1Member_01a0582c-fc0b-7a25-9036-9c513be5f64a" xlink:href="jbsay-20260930.xsd#jbsay_RevolvingCreditFacility1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fc0b-7216-92a1-4c4ecb56148c" xlink:to="loc_jbsay_RevolvingCreditFacility1Member_01a0582c-fc0b-7a25-9036-9c513be5f64a" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposureToTheCdiRateAbstract_01a0582c-fc0b-7996-a7d0-3351a3a31c08" xlink:href="jbsay-20260930.xsd#jbsay_NetExposureToTheCdiRateAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems_01a0582c-fc0b-74f2-a0aa-710ef54a7018" xlink:to="loc_jbsay_NetExposureToTheCdiRateAbstract_01a0582c-fc0b-7996-a7d0-3351a3a31c08" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposureToTheCDIRate_01a0a4e2-122c-791c-96af-9ff4b9d3e0a9" xlink:href="jbsay-20260930.xsd#jbsay_NetExposureToTheCDIRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_NetExposureToTheCdiRateAbstract_01a0582c-fc0b-7996-a7d0-3351a3a31c08" xlink:to="loc_jbsay_NetExposureToTheCDIRate_01a0a4e2-122c-791c-96af-9ff4b9d3e0a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposureToTheIpcaRateAbstract_01a0582c-fc0b-7c24-ad4e-bbf31a92bd20" xlink:href="jbsay-20260930.xsd#jbsay_NetExposureToTheIpcaRateAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems_01a0582c-fc0b-74f2-a0aa-710ef54a7018" xlink:to="loc_jbsay_NetExposureToTheIpcaRateAbstract_01a0582c-fc0b-7c24-ad4e-bbf31a92bd20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposureToTheIPCARate_01a0a4e2-122c-7489-ab2d-09f27e97f7bf" xlink:href="jbsay-20260930.xsd#jbsay_NetExposureToTheIPCARate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_NetExposureToTheIpcaRateAbstract_01a0582c-fc0b-7c24-ad4e-bbf31a92bd20" xlink:to="loc_jbsay_NetExposureToTheIPCARate_01a0a4e2-122c-7489-ab2d-09f27e97f7bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LiabilitiesExposureToTheSofrRateAbstract_01a0582c-fc0b-7682-b0a8-4ec511282b55" xlink:href="jbsay-20260930.xsd#jbsay_LiabilitiesExposureToTheSofrRateAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems_01a0582c-fc0b-74f2-a0aa-710ef54a7018" xlink:to="loc_jbsay_LiabilitiesExposureToTheSofrRateAbstract_01a0582c-fc0b-7682-b0a8-4ec511282b55" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LiabilitiesExposureToTheSOFRRates_01a0a4e2-122c-7826-9f80-92b338ee0b32" xlink:href="jbsay-20260930.xsd#jbsay_LiabilitiesExposureToTheSOFRRates"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_LiabilitiesExposureToTheSofrRateAbstract_01a0582c-fc0b-7682-b0a8-4ec511282b55" xlink:to="loc_jbsay_LiabilitiesExposureToTheSOFRRates_01a0a4e2-122c-7826-9f80-92b338ee0b32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LiabilitiesExposureToTheEuriborRateAbstract_01a0582c-fc0b-7703-8c3f-3a977a2cdcd1" xlink:href="jbsay-20260930.xsd#jbsay_LiabilitiesExposureToTheEuriborRateAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofAssetsandLiabilitiesExposedtoFloatingInterestRatesDetailsLineItems_01a0582c-fc0b-74f2-a0aa-710ef54a7018" xlink:to="loc_jbsay_LiabilitiesExposureToTheEuriborRateAbstract_01a0582c-fc0b-7703-8c3f-3a977a2cdcd1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_LiabilitiesExposureToTheEuriborRates_01a0582c-fc0b-7c5c-b270-ca431b754488" xlink:href="jbsay-20260930.xsd#jbsay_LiabilitiesExposureToTheEuriborRates"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_LiabilitiesExposureToTheEuriborRateAbstract_01a0582c-fc0b-7703-8c3f-3a977a2cdcd1" xlink:to="loc_jbsay_LiabilitiesExposureToTheEuriborRates_01a0582c-fc0b-7c5c-b270-ca431b754488" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofContractsExposureScenarioDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofContractsExposureScenarioDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofContractsExposureScenarioDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScheduleOfContractsExposureScenarioLineItems_01a0582c-fc0b-7906-8ed2-bf1a862cd8e1" xlink:href="jbsay-20260930.xsd#jbsay_ScheduleOfContractsExposureScenarioLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7936-b0d9-c2dc67c48b14" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ScheduleOfContractsExposureScenarioLineItems_01a0582c-fc0b-7906-8ed2-bf1a862cd8e1" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7936-b0d9-c2dc67c48b14" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-7c28-9ebe-8129cc0512ab" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7936-b0d9-c2dc67c48b14" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-7c28-9ebe-8129cc0512ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7c28-9ebe-8129cc0512ab_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-7c28-9ebe-8129cc0512ab" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7c28-9ebe-8129cc0512ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7079-be27-7af6502cf719" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-7c28-9ebe-8129cc0512ab" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7079-be27-7af6502cf719" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CDIMember_01a0582c-fc0b-706a-89d2-6e129b522b50" xlink:href="jbsay-20260930.xsd#jbsay_CDIMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7079-be27-7af6502cf719" xlink:to="loc_jbsay_CDIMember_01a0582c-fc0b-706a-89d2-6e129b522b50" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_IPCAMember_01a0582c-fc0b-70dd-8635-31a51de7fd61" xlink:href="jbsay-20260930.xsd#jbsay_IPCAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7079-be27-7af6502cf719" xlink:to="loc_jbsay_IPCAMember_01a0582c-fc0b-70dd-8635-31a51de7fd61" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SOFRMember_01a0582c-fc0b-736d-88e2-168f5d910306" xlink:href="jbsay-20260930.xsd#jbsay_SOFRMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7079-be27-7af6502cf719" xlink:to="loc_jbsay_SOFRMember_01a0582c-fc0b-736d-88e2-168f5d910306" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_EuriborMember_01a0582c-fc0b-77b1-a5ab-45a7268db395" xlink:href="jbsay-20260930.xsd#jbsay_EuriborMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7079-be27-7af6502cf719" xlink:to="loc_jbsay_EuriborMember_01a0582c-fc0b-77b1-a5ab-45a7268db395" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-730a-90a6-0bfa91e8df71" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7936-b0d9-c2dc67c48b14" xlink:to="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-730a-90a6-0bfa91e8df71" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-730a-90a6-0bfa91e8df71_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-730a-90a6-0bfa91e8df71" xlink:to="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-730a-90a6-0bfa91e8df71_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7985-ad0b-3eda145106dc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-730a-90a6-0bfa91e8df71" xlink:to="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7985-ad0b-3eda145106dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentScenarioMember_01a0582c-fc0b-7e2b-a731-e821f5d0351e" xlink:href="jbsay-20260930.xsd#jbsay_CurrentScenarioMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7985-ad0b-3eda145106dc" xlink:to="loc_jbsay_CurrentScenarioMember_01a0582c-fc0b-7e2b-a731-e821f5d0351e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIVaR99I.C.1DayMember_01a0582c-fc0b-7409-ac3a-756cb1760184" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIVaR99I.C.1DayMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7985-ad0b-3eda145106dc" xlink:to="loc_jbsay_ScenarioIVaR99I.C.1DayMember_01a0582c-fc0b-7409-ac3a-756cb1760184" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIiInterestRateVariation25Member_01a0582c-fc0b-7ca7-ad39-601ca2b48632" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIiInterestRateVariation25Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7985-ad0b-3eda145106dc" xlink:to="loc_jbsay_ScenarioIiInterestRateVariation25Member_01a0582c-fc0b-7ca7-ad39-601ca2b48632" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIiiInterestRateVariation50Member_01a0582c-fc0b-7bde-af39-3a71a017ff97" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIiiInterestRateVariation50Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7985-ad0b-3eda145106dc" xlink:to="loc_jbsay_ScenarioIiiInterestRateVariation50Member_01a0582c-fc0b-7bde-af39-3a71a017ff97" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PercentageOfCurrentScenario_01a0582c-fc0b-7147-8cd0-39947623f0fc" xlink:href="jbsay-20260930.xsd#jbsay_PercentageOfCurrentScenario"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfContractsExposureScenarioLineItems_01a0582c-fc0b-7906-8ed2-bf1a862cd8e1" xlink:to="loc_jbsay_PercentageOfCurrentScenario_01a0582c-fc0b-7147-8cd0-39947623f0fc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PercentageOfExchangeRate_01a0582c-fc0b-787c-8640-0e7009c65210" xlink:href="jbsay-20260930.xsd#jbsay_PercentageOfExchangeRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfContractsExposureScenarioLineItems_01a0582c-fc0b-7906-8ed2-bf1a862cd8e1" xlink:to="loc_jbsay_PercentageOfExchangeRate_01a0582c-fc0b-787c-8640-0e7009c65210" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_EffectOnIncome_01a0582c-fc0b-7f95-9c98-2dc2503d4f2a" xlink:href="jbsay-20260930.xsd#jbsay_EffectOnIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfContractsExposureScenarioLineItems_01a0582c-fc0b-7906-8ed2-bf1a862cd8e1" xlink:to="loc_jbsay_EffectOnIncome_01a0582c-fc0b-7f95-9c98-2dc2503d4f2a" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofExposureDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofExposureDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofExposureDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsTable_01a0582c-fc0b-7f7a-8d83-e7a17dd9993b" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsTable_01a0582c-fc0b-7f7a-8d83-e7a17dd9993b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7806-8931-3f8a7939a36b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsTable_01a0582c-fc0b-7f7a-8d83-e7a17dd9993b" xlink:to="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7806-8931-3f8a7939a36b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7806-8931-3f8a7939a36b_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7806-8931-3f8a7939a36b" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7806-8931-3f8a7939a36b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-79ae-a04a-02cd4a3d7e34" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7806-8931-3f8a7939a36b" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-79ae-a04a-02cd4a3d7e34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UnitedStatesOfAmericaDollarsMember_01a0582c-fc0b-7826-a790-1faea1052587" xlink:href="jbsay-20260930.xsd#jbsay_UnitedStatesOfAmericaDollarsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-79ae-a04a-02cd4a3d7e34" xlink:to="loc_jbsay_UnitedStatesOfAmericaDollarsMember_01a0582c-fc0b-7826-a790-1faea1052587" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_EuroMemberCountriesEuroMember_01a0582c-fc0b-7a74-8506-bdc9cf4f70ef" xlink:href="jbsay-20260930.xsd#jbsay_EuroMemberCountriesEuroMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-79ae-a04a-02cd4a3d7e34" xlink:to="loc_jbsay_EuroMemberCountriesEuroMember_01a0582c-fc0b-7a74-8506-bdc9cf4f70ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UnitedKingdomPoundsMember_01a0582c-fc0b-7ff5-88bc-d9ac08d54d0d" xlink:href="jbsay-20260930.xsd#jbsay_UnitedKingdomPoundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-79ae-a04a-02cd4a3d7e34" xlink:to="loc_jbsay_UnitedKingdomPoundsMember_01a0582c-fc0b-7ff5-88bc-d9ac08d54d0d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingSubtotal_01a0582c-fc0b-7dcb-87ce-bf8a4b2d0510" xlink:href="jbsay-20260930.xsd#jbsay_OperatingSubtotal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:to="loc_jbsay_OperatingSubtotal_01a0582c-fc0b-7dcb-87ce-bf8a4b2d0510" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FinancialSubtotal_01a0582c-fc0b-7ecb-82d6-470dea23db31" xlink:href="jbsay-20260930.xsd#jbsay_FinancialSubtotal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:to="loc_jbsay_FinancialSubtotal_01a0582c-fc0b-7ecb-82d6-470dea23db31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_TotalExposure_01a0582c-fc0b-7d31-a8bc-66d3a072ba63" xlink:href="jbsay-20260930.xsd#jbsay_TotalExposure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:to="loc_jbsay_TotalExposure_01a0582c-fc0b-7d31-a8bc-66d3a072ba63" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssets_01a0582c-fc0b-7b99-a1a5-c937eef622f7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:to="loc_ifrs-full_FinancialAssets_01a0582c-fc0b-7b99-a1a5-c937eef622f7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposure_01a0a4fe-f50f-72aa-9c78-007f50176c86" xlink:href="jbsay-20260930.xsd#jbsay_NetExposure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureDetailsLineItems_01a0582c-fc0b-76ba-b7c1-b570de7efade" xlink:to="loc_jbsay_NetExposure_01a0a4fe-f50f-72aa-9c78-007f50176c86" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofSensitivityAnalysisandDerivativeFinancialInstrumentsBreakdownDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofSensitivityAnalysisandDerivativeFinancialInstrumentsBreakdownDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofSensitivityAnalysisandDerivativeFinancialInstrumentsBreakdownDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7299-b314-7ef528df8c57" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7407-bed1-f5820df5a0bb" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7299-b314-7ef528df8c57" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7407-bed1-f5820df5a0bb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CurrencyAxis_01a0582c-fc0b-77ed-af0d-82d1b3647eec" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_CurrencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7407-bed1-f5820df5a0bb" xlink:to="loc_srt_CurrencyAxis_01a0582c-fc0b-77ed-af0d-82d1b3647eec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_currency_AllCurrenciesDomain_01a0582c-fc0b-77ed-af0d-82d1b3647eec_default" xlink:href="https://xbrl.sec.gov/currency/2025/currency-2025.xsd#currency_AllCurrenciesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CurrencyAxis_01a0582c-fc0b-77ed-af0d-82d1b3647eec" xlink:to="loc_currency_AllCurrenciesDomain_01a0582c-fc0b-77ed-af0d-82d1b3647eec_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_currency_AllCurrenciesDomain_01a0582c-fc0b-7dc5-bfd5-dc05082c10f4" xlink:href="https://xbrl.sec.gov/currency/2025/currency-2025.xsd#currency_AllCurrenciesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CurrencyAxis_01a0582c-fc0b-77ed-af0d-82d1b3647eec" xlink:to="loc_currency_AllCurrenciesDomain_01a0582c-fc0b-7dc5-bfd5-dc05082c10f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_currency_USD_01a0582c-fc0b-786c-bd3a-f9881ae2d854" xlink:href="https://xbrl.sec.gov/currency/2025/currency-2025.xsd#currency_USD"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_currency_AllCurrenciesDomain_01a0582c-fc0b-7dc5-bfd5-dc05082c10f4" xlink:to="loc_currency_USD_01a0582c-fc0b-786c-bd3a-f9881ae2d854" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-77e3-85ab-fea1d094614a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7407-bed1-f5820df5a0bb" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-77e3-85ab-fea1d094614a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-77e3-85ab-fea1d094614a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-77e3-85ab-fea1d094614a" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-77e3-85ab-fea1d094614a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7e0f-a9e2-ce507bcd4d2f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fc0b-77e3-85ab-fea1d094614a" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7e0f-a9e2-ce507bcd4d2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingMember_01a0582c-fc0b-7163-b44e-4d92a272fd4b" xlink:href="jbsay-20260930.xsd#jbsay_OperatingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7e0f-a9e2-ce507bcd4d2f" xlink:to="loc_jbsay_OperatingMember_01a0582c-fc0b-7163-b44e-4d92a272fd4b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FinancialMember_01a0582c-fc0b-7481-a729-57eaedec9c71" xlink:href="jbsay-20260930.xsd#jbsay_FinancialMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7e0f-a9e2-ce507bcd4d2f" xlink:to="loc_jbsay_FinancialMember_01a0582c-fc0b-7481-a729-57eaedec9c71" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DerivativesMember_01a0582c-fc0b-7420-8af0-c5416a6c9c8f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DerivativesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fc0b-7e0f-a9e2-ce507bcd4d2f" xlink:to="loc_ifrs-full_DerivativesMember_01a0582c-fc0b-7420-8af0-c5416a6c9c8f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-7103-bb8f-9b131b0eb79d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7407-bed1-f5820df5a0bb" xlink:to="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-7103-bb8f-9b131b0eb79d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7103-bb8f-9b131b0eb79d_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-7103-bb8f-9b131b0eb79d" xlink:to="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7103-bb8f-9b131b0eb79d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7171-bbec-bfbdd8f0a2cd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RiskExposuresAxis_01a0582c-fc0b-7103-bb8f-9b131b0eb79d" xlink:to="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7171-bbec-bfbdd8f0a2cd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentExchangeRateMember_01a0582c-fc0b-7c68-8102-f5697ccaceb4" xlink:href="jbsay-20260930.xsd#jbsay_CurrentExchangeRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7171-bbec-bfbdd8f0a2cd" xlink:to="loc_jbsay_CurrentExchangeRateMember_01a0582c-fc0b-7c68-8102-f5697ccaceb4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIVaR99C.I.1DayMember_01a0582c-fc0b-7197-bf42-e61ffc9b60c3" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIVaR99C.I.1DayMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7171-bbec-bfbdd8f0a2cd" xlink:to="loc_jbsay_ScenarioIVaR99C.I.1DayMember_01a0582c-fc0b-7197-bf42-e61ffc9b60c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIiInterestRateVariation15Member_01a0582c-fc0b-740d-9d30-44e7c2c177d3" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIiInterestRateVariation15Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7171-bbec-bfbdd8f0a2cd" xlink:to="loc_jbsay_ScenarioIiInterestRateVariation15Member_01a0582c-fc0b-740d-9d30-44e7c2c177d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIiiInterestRateVariation30Member_01a0582c-fc0b-7d70-a36c-7b8c2b91c2d4" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIiiInterestRateVariation30Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fc0b-7171-bbec-bfbdd8f0a2cd" xlink:to="loc_jbsay_ScenarioIiiInterestRateVariation30Member_01a0582c-fc0b-7d70-a36c-7b8c2b91c2d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7fd2-b6ce-e525826372cf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fc0b-7407-bed1-f5820df5a0bb" xlink:to="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7fd2-b6ce-e525826372cf" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7fd2-b6ce-e525826372cf_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7fd2-b6ce-e525826372cf" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7fd2-b6ce-e525826372cf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7c17-ac3c-0da3b4a2e45b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeographicalAreasDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_GeographicalAreasAxis_01a0582c-fc0b-7fd2-b6ce-e525826372cf" xlink:to="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7c17-ac3c-0da3b4a2e45b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_EuroMemberCountriesEuroMember_01a0582c-fc0b-70d8-a6bd-9bbbe2e35128" xlink:href="jbsay-20260930.xsd#jbsay_EuroMemberCountriesEuroMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7c17-ac3c-0da3b4a2e45b" xlink:to="loc_jbsay_EuroMemberCountriesEuroMember_01a0582c-fc0b-70d8-a6bd-9bbbe2e35128" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UnitedKingdomPoundsMember_01a0582c-fc0b-74e8-b1e9-067bf657ce49" xlink:href="jbsay-20260930.xsd#jbsay_UnitedKingdomPoundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_GeographicalAreasDomain_01a0582c-fc0b-7c17-ac3c-0da3b4a2e45b" xlink:to="loc_jbsay_UnitedKingdomPoundsMember_01a0582c-fc0b-74e8-b1e9-067bf657ce49" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PercentageOfCurrentScenario_01a0582c-fcd3-7ce3-8eca-a5d4b7e6d706" xlink:href="jbsay-20260930.xsd#jbsay_PercentageOfCurrentScenario"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7299-b314-7ef528df8c57" xlink:to="loc_jbsay_PercentageOfCurrentScenario_01a0582c-fcd3-7ce3-8eca-a5d4b7e6d706" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PercentageOfExchangeRate_01a0582c-fcd3-7913-9f55-6130b2acfc09" xlink:href="jbsay-20260930.xsd#jbsay_PercentageOfExchangeRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7299-b314-7ef528df8c57" xlink:to="loc_jbsay_PercentageOfExchangeRate_01a0582c-fcd3-7913-9f55-6130b2acfc09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_EffectOnIncome_01a0582c-fcd3-778d-ad40-972d620e17b4" xlink:href="jbsay-20260930.xsd#jbsay_EffectOnIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7299-b314-7ef528df8c57" xlink:to="loc_jbsay_EffectOnIncome_01a0582c-fcd3-778d-ad40-972d620e17b4" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofFutureContractsDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofFutureContractsDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofFutureContractsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsLineItems_01a0582c-fcd3-7f85-9a99-33fb86ba7941" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsTable_01a0582c-fcd3-7bc7-90f0-4a560c611497" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsLineItems_01a0582c-fcd3-7f85-9a99-33fb86ba7941" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsTable_01a0582c-fcd3-7bc7-90f0-4a560c611497" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77af-8948-d39953a73009" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsTable_01a0582c-fcd3-7bc7-90f0-4a560c611497" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77af-8948-d39953a73009" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-77af-8948-d39953a73009_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77af-8948-d39953a73009" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-77af-8948-d39953a73009_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77af-8948-d39953a73009" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ForwardContractMember_01a0582c-fcd3-7628-a3e2-3171e1c5339c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ForwardContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_ifrs-full_ForwardContractMember_01a0582c-fcd3-7628-a3e2-3171e1c5339c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeliverableForwardsMember_01a0582c-fcd3-7433-ae02-323d89e3e7ba" xlink:href="jbsay-20260930.xsd#jbsay_DeliverableForwardsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_DeliverableForwardsMember_01a0582c-fcd3-7433-ae02-323d89e3e7ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonDeliverableForwardsMember_01a0582c-fcd3-7ae4-8aa1-50b916a7af81" xlink:href="jbsay-20260930.xsd#jbsay_NonDeliverableForwardsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_NonDeliverableForwardsMember_01a0582c-fcd3-7ae4-8aa1-50b916a7af81" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FutureContractOneMember_01a0582c-fcd3-794a-a4a9-c286c023fd41" xlink:href="jbsay-20260930.xsd#jbsay_FutureContractOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_FutureContractOneMember_01a0582c-fcd3-794a-a4a9-c286c023fd41" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeliverableForwardsOneMember_01a0582c-fcd3-7263-944f-be3e94b9ce1d" xlink:href="jbsay-20260930.xsd#jbsay_DeliverableForwardsOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_DeliverableForwardsOneMember_01a0582c-fcd3-7263-944f-be3e94b9ce1d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonDeliverableForwardsOneMember_01a0582c-fcd3-702f-98fc-abeaf1a30c2b" xlink:href="jbsay-20260930.xsd#jbsay_NonDeliverableForwardsOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_NonDeliverableForwardsOneMember_01a0582c-fcd3-702f-98fc-abeaf1a30c2b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FutureContractTwoMember_01a0582c-fcd3-7a4f-9eba-a225a71831e2" xlink:href="jbsay-20260930.xsd#jbsay_FutureContractTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_FutureContractTwoMember_01a0582c-fcd3-7a4f-9eba-a225a71831e2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeliverableForwardsTwoMember_01a0582c-fcd3-7e20-a8e7-bb2532c11384" xlink:href="jbsay-20260930.xsd#jbsay_DeliverableForwardsTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-79a7-a8bf-4d8baeac9ac3" xlink:to="loc_jbsay_DeliverableForwardsTwoMember_01a0582c-fcd3-7e20-a8e7-bb2532c11384" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7ec5-bd72-09f05077b964" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsTable_01a0582c-fcd3-7bc7-90f0-4a560c611497" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7ec5-bd72-09f05077b964" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7ec5-bd72-09f05077b964_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7ec5-bd72-09f05077b964" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7ec5-bd72-09f05077b964_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7b58-8a59-67363358371a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7ec5-bd72-09f05077b964" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7b58-8a59-67363358371a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SensitivityAnalysisAndDerivativeFinancialInstrumentBreakdownMember_01a0582c-fcd3-785d-bdae-7ac7631dda8a" xlink:href="jbsay-20260930.xsd#jbsay_SensitivityAnalysisAndDerivativeFinancialInstrumentBreakdownMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7b58-8a59-67363358371a" xlink:to="loc_jbsay_SensitivityAnalysisAndDerivativeFinancialInstrumentBreakdownMember_01a0582c-fcd3-785d-bdae-7ac7631dda8a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfNatureOfContingentAssets_01a0582c-fcd3-75f5-a244-6a30aef13403" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DescriptionOfNatureOfContingentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsLineItems_01a0582c-fcd3-7f85-9a99-33fb86ba7941" xlink:to="loc_ifrs-full_DescriptionOfNatureOfContingentAssets_01a0582c-fcd3-75f5-a244-6a30aef13403" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NotionalAmount_01a0582c-fcd3-765c-9fee-3cb2d47a515b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsLineItems_01a0582c-fcd3-7f85-9a99-33fb86ba7941" xlink:to="loc_ifrs-full_NotionalAmount_01a0582c-fcd3-765c-9fee-3cb2d47a515b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssetsAtFairValue_01a0582c-fcd3-7a94-b239-4f05e2d04f84" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinancialAssetsAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofFutureContractsDetailsLineItems_01a0582c-fcd3-7f85-9a99-33fb86ba7941" xlink:to="loc_ifrs-full_FinancialAssetsAtFairValue_01a0582c-fcd3-7a94-b239-4f05e2d04f84" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofExposureinCommoditiesDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofExposureinCommoditiesDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofExposureinCommoditiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScheduleOfExposureInCommoditiesLineItems_01a0582c-fcd3-7572-9a0b-9b1320afd5b7" xlink:href="jbsay-20260930.xsd#jbsay_ScheduleOfExposureInCommoditiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureinCommoditiesDetailsTable_01a0582c-fcd3-7d0e-9a1b-3609cce04962" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureinCommoditiesDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_ScheduleOfExposureInCommoditiesLineItems_01a0582c-fcd3-7572-9a0b-9b1320afd5b7" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureinCommoditiesDetailsTable_01a0582c-fcd3-7d0e-9a1b-3609cce04962" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-7d5d-8e7e-0146fffd7c58" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureinCommoditiesDetailsTable_01a0582c-fcd3-7d0e-9a1b-3609cce04962" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-7d5d-8e7e-0146fffd7c58" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7d5d-8e7e-0146fffd7c58_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-7d5d-8e7e-0146fffd7c58" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7d5d-8e7e-0146fffd7c58_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7f06-80af-a5d14f8864cf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-7d5d-8e7e-0146fffd7c58" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7f06-80af-a5d14f8864cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CattleMember_01a0582c-fcd3-777a-8a73-a1c12b692e00" xlink:href="jbsay-20260930.xsd#jbsay_CattleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7f06-80af-a5d14f8864cf" xlink:to="loc_jbsay_CattleMember_01a0582c-fcd3-777a-8a73-a1c12b692e00" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7496-accc-ac4f51ab89b2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofExposureinCommoditiesDetailsTable_01a0582c-fcd3-7d0e-9a1b-3609cce04962" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7496-accc-ac4f51ab89b2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7496-accc-ac4f51ab89b2_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7496-accc-ac4f51ab89b2" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7496-accc-ac4f51ab89b2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7496-accc-ac4f51ab89b2" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FirmContractsOfCattlePurchaseMember_01a0582c-fcd3-74b5-b3f8-a0284ce86931" xlink:href="jbsay-20260930.xsd#jbsay_FirmContractsOfCattlePurchaseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:to="loc_jbsay_FirmContractsOfCattlePurchaseMember_01a0582c-fcd3-74b5-b3f8-a0284ce86931" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DerivativesSubtotalMember_01a0582c-fcd3-7652-9f74-e64a914dc364" xlink:href="jbsay-20260930.xsd#jbsay_DerivativesSubtotalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:to="loc_jbsay_DerivativesSubtotalMember_01a0582c-fcd3-7652-9f74-e64a914dc364" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FuturesContractMember_01a0582c-fcd3-73bc-bf4e-84ce228b360d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FuturesContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:to="loc_ifrs-full_FuturesContractMember_01a0582c-fcd3-73bc-bf4e-84ce228b360d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ForwardContractMember_01a0f157-2a7a-7c44-a273-1bd2f3df13cc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ForwardContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:to="loc_ifrs-full_ForwardContractMember_01a0f157-2a7a-7c44-a273-1bd2f3df13cc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonDeliverableForwardsMember_01a18827-ea2c-7e0e-961a-247a2a81a49f" xlink:href="jbsay-20260930.xsd#jbsay_NonDeliverableForwardsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:to="loc_jbsay_NonDeliverableForwardsMember_01a18827-ea2c-7e0e-961a-247a2a81a49f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ExposureSubtotalMember_01a0582c-fcd3-7da1-89a0-e21896dd689c" xlink:href="jbsay-20260930.xsd#jbsay_ExposureSubtotalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78dc-9ada-57d109fafb73" xlink:to="loc_jbsay_ExposureSubtotalMember_01a0582c-fcd3-7da1-89a0-e21896dd689c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposureValue_01a0582c-fcd3-7ba1-8590-95d007b5cc42" xlink:href="jbsay-20260930.xsd#jbsay_NetExposureValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_ScheduleOfExposureInCommoditiesLineItems_01a0582c-fcd3-7572-9a0b-9b1320afd5b7" xlink:to="loc_jbsay_NetExposureValue_01a0582c-fcd3-7ba1-8590-95d007b5cc42" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofSensitivityAnalysisDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofSensitivityAnalysisDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofSensitivityAnalysisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7b0a-9e5d-87eaae4fd131" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7c40-9d0f-23bcb212636f" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7b0a-9e5d-87eaae4fd131" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7c40-9d0f-23bcb212636f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-71d0-a55f-6603aa03125c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7c40-9d0f-23bcb212636f" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-71d0-a55f-6603aa03125c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-71d0-a55f-6603aa03125c_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-71d0-a55f-6603aa03125c" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-71d0-a55f-6603aa03125c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7015-a6f9-70fbf6b854ee" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-71d0-a55f-6603aa03125c" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7015-a6f9-70fbf6b854ee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CattleMember_01a0582c-fcd3-7cfd-b3b6-5a70d080c7c9" xlink:href="jbsay-20260930.xsd#jbsay_CattleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7015-a6f9-70fbf6b854ee" xlink:to="loc_jbsay_CattleMember_01a0582c-fcd3-7cfd-b3b6-5a70d080c7c9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CommoditiesGrainAndOthersMember_01a0582c-fcd3-76a1-a759-cdaac02460d7" xlink:href="jbsay-20260930.xsd#jbsay_CommoditiesGrainAndOthersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7015-a6f9-70fbf6b854ee" xlink:to="loc_jbsay_CommoditiesGrainAndOthersMember_01a0582c-fcd3-76a1-a759-cdaac02460d7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-78ea-8ed7-326f9064ede1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7c40-9d0f-23bcb212636f" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-78ea-8ed7-326f9064ede1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78ea-8ed7-326f9064ede1_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-78ea-8ed7-326f9064ede1" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-78ea-8ed7-326f9064ede1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-74f4-9e17-9476435e620d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-78ea-8ed7-326f9064ede1" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-74f4-9e17-9476435e620d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingMember_01a0582c-fcd3-7857-9dcd-46c7b0adb5e1" xlink:href="jbsay-20260930.xsd#jbsay_OperatingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-74f4-9e17-9476435e620d" xlink:to="loc_jbsay_OperatingMember_01a0582c-fcd3-7857-9dcd-46c7b0adb5e1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DerivativesMember_01a0582c-fcd3-77ba-8603-4464a3840df9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DerivativesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-74f4-9e17-9476435e620d" xlink:to="loc_ifrs-full_DerivativesMember_01a0582c-fcd3-77ba-8603-4464a3840df9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresAxis_01a0582c-fcd3-77a0-9e90-eb5adf121e52" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7c40-9d0f-23bcb212636f" xlink:to="loc_ifrs-full_RiskExposuresAxis_01a0582c-fcd3-77a0-9e90-eb5adf121e52" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-77a0-9e90-eb5adf121e52_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_RiskExposuresAxis_01a0582c-fcd3-77a0-9e90-eb5adf121e52" xlink:to="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-77a0-9e90-eb5adf121e52_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-76cd-9e8c-daa885d7efb8" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RiskExposuresDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_RiskExposuresAxis_01a0582c-fcd3-77a0-9e90-eb5adf121e52" xlink:to="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-76cd-9e8c-daa885d7efb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentPriceMember_01a0582c-fcd3-7b2c-bc01-6eb351f446f3" xlink:href="jbsay-20260930.xsd#jbsay_CurrentPriceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-76cd-9e8c-daa885d7efb8" xlink:to="loc_jbsay_CurrentPriceMember_01a0582c-fcd3-7b2c-bc01-6eb351f446f3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIVaR99C.I.1DayMember_01a0582c-fcd3-7954-b514-7328770c27ee" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIVaR99C.I.1DayMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-76cd-9e8c-daa885d7efb8" xlink:to="loc_jbsay_ScenarioIVaR99C.I.1DayMember_01a0582c-fcd3-7954-b514-7328770c27ee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIiInterestRateVariation15Member_01a0582c-fcd3-7fa2-8f84-04b96483480c" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIiInterestRateVariation15Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-76cd-9e8c-daa885d7efb8" xlink:to="loc_jbsay_ScenarioIiInterestRateVariation15Member_01a0582c-fcd3-7fa2-8f84-04b96483480c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ScenarioIiiInterestRateVariation30Member_01a0582c-fcd3-78eb-bbd5-253708abfd4c" xlink:href="jbsay-20260930.xsd#jbsay_ScenarioIiiInterestRateVariation30Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_RiskExposuresDomain_01a0582c-fcd3-76cd-9e8c-daa885d7efb8" xlink:to="loc_jbsay_ScenarioIiiInterestRateVariation30Member_01a0582c-fcd3-78eb-bbd5-253708abfd4c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_EffectOnIncome_01a0582c-fcd3-7d0c-83d3-eea1ad0b3e06" xlink:href="jbsay-20260930.xsd#jbsay_EffectOnIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7b0a-9e5d-87eaae4fd131" xlink:to="loc_jbsay_EffectOnIncome_01a0582c-fcd3-7d0c-83d3-eea1ad0b3e06" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ValueAtRiskExchangePrice_01a0582c-fcd3-783a-b173-112f8d8d814e" xlink:href="jbsay-20260930.xsd#jbsay_ValueAtRiskExchangePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7b0a-9e5d-87eaae4fd131" xlink:to="loc_jbsay_ValueAtRiskExchangePrice_01a0582c-fcd3-783a-b173-112f8d8d814e" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofDerivativesFinancialInstrumentsBreakdownDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofDerivativesFinancialInstrumentsBreakdownDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofDerivativesFinancialInstrumentsBreakdownDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7476-83f0-3486b6ee56c3" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7740-915f-831adedb7dab" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7476-83f0-3486b6ee56c3" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7740-915f-831adedb7dab" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77f8-9b65-42b7c3ae6424" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7740-915f-831adedb7dab" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77f8-9b65-42b7c3ae6424" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-77f8-9b65-42b7c3ae6424_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77f8-9b65-42b7c3ae6424" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-77f8-9b65-42b7c3ae6424_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fef-9446-a2d27aa20de3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-77f8-9b65-42b7c3ae6424" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fef-9446-a2d27aa20de3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FutureContractsSixMember_01a0582c-fcd3-75da-a075-ba636c38b1b6" xlink:href="jbsay-20260930.xsd#jbsay_FutureContractsSixMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fef-9446-a2d27aa20de3" xlink:to="loc_jbsay_FutureContractsSixMember_01a0582c-fcd3-75da-a075-ba636c38b1b6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeliverableForwardsFiveMember_01a0582c-fcd3-78ad-9c08-6610f0182bd5" xlink:href="jbsay-20260930.xsd#jbsay_DeliverableForwardsFiveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fef-9446-a2d27aa20de3" xlink:to="loc_jbsay_DeliverableForwardsFiveMember_01a0582c-fcd3-78ad-9c08-6610f0182bd5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FutureCMEMember_01a0582c-fcd3-7648-9881-51e4d70c8c75" xlink:href="jbsay-20260930.xsd#jbsay_FutureCMEMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fef-9446-a2d27aa20de3" xlink:to="loc_jbsay_FutureCMEMember_01a0582c-fcd3-7648-9881-51e4d70c8c75" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonDeliverableForwardsMember_01a0582c-fcd3-7bfa-a3c1-f48a4a499a29" xlink:href="jbsay-20260930.xsd#jbsay_NonDeliverableForwardsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fef-9446-a2d27aa20de3" xlink:to="loc_jbsay_NonDeliverableForwardsMember_01a0582c-fcd3-7bfa-a3c1-f48a4a499a29" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7d2e-8614-e6b742da8488" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7740-915f-831adedb7dab" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7d2e-8614-e6b742da8488" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7d2e-8614-e6b742da8488_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7d2e-8614-e6b742da8488" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-7d2e-8614-e6b742da8488_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-72a9-ab74-734fad1e7331" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsAxis_01a0582c-fcd3-7d2e-8614-e6b742da8488" xlink:to="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-72a9-ab74-734fad1e7331" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_SensitivityAnalysisAndDerivativeFinancialInstrumentBreakdownMember_01a0582c-fcd3-730d-abba-937cebb0d811" xlink:href="jbsay-20260930.xsd#jbsay_SensitivityAnalysisAndDerivativeFinancialInstrumentBreakdownMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialInstrumentsDomain_01a0582c-fcd3-72a9-ab74-734fad1e7331" xlink:to="loc_jbsay_SensitivityAnalysisAndDerivativeFinancialInstrumentBreakdownMember_01a0582c-fcd3-730d-abba-937cebb0d811" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fcd3-76f4-a284-9dc3a5fb4116" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrencyInWhichInformationIsDisplayedAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7740-915f-831adedb7dab" xlink:to="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fcd3-76f4-a284-9dc3a5fb4116" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fcd3-76f4-a284-9dc3a5fb4116_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FunctionalOrPresentationCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fcd3-76f4-a284-9dc3a5fb4116" xlink:to="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fcd3-76f4-a284-9dc3a5fb4116_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fcd3-7c43-b09e-a7f0964c1e67" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FunctionalOrPresentationCurrencyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CurrencyInWhichInformationIsDisplayedAxis_01a0582c-fcd3-76f4-a284-9dc3a5fb4116" xlink:to="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fcd3-7c43-b09e-a7f0964c1e67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CommoditiesCattleMember_01a0a515-abc3-7237-af11-76fab51915d0" xlink:href="jbsay-20260930.xsd#jbsay_CommoditiesCattleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fcd3-7c43-b09e-a7f0964c1e67" xlink:to="loc_jbsay_CommoditiesCattleMember_01a0a515-abc3-7237-af11-76fab51915d0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CommoditiesGrainAndOthersMember_01a0582c-fcd3-7c41-be77-9b43bd554552" xlink:href="jbsay-20260930.xsd#jbsay_CommoditiesGrainAndOthersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_FunctionalOrPresentationCurrencyMember_01a0582c-fcd3-7c43-b09e-a7f0964c1e67" xlink:to="loc_jbsay_CommoditiesGrainAndOthersMember_01a0582c-fcd3-7c41-be77-9b43bd554552" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DescriptionOfNatureOfContingentAssets_01a0582c-fcd3-7e22-aae7-ad139c7b29af" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DescriptionOfNatureOfContingentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7476-83f0-3486b6ee56c3" xlink:to="loc_ifrs-full_DescriptionOfNatureOfContingentAssets_01a0582c-fcd3-7e22-aae7-ad139c7b29af" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NumberOfDerivatives_01a1b231-aa3d-7550-9674-1337c47e9c2c" xlink:href="jbsay-20260930.xsd#jbsay_NumberOfDerivatives"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7476-83f0-3486b6ee56c3" xlink:to="loc_jbsay_NumberOfDerivatives_01a1b231-aa3d-7550-9674-1337c47e9c2c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NumberOfSharesOutstanding_01a0582c-fcd3-719f-9fad-2c0f31fc2697" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NumberOfSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7476-83f0-3486b6ee56c3" xlink:to="loc_ifrs-full_NumberOfSharesOutstanding_01a0582c-fcd3-719f-9fad-2c0f31fc2697" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinancialAssetsAtFairValue_01a0582c-fcd3-7924-94b2-3d0128623b8e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinancialAssetsAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7476-83f0-3486b6ee56c3" xlink:to="loc_ifrs-full_FinancialAssetsAtFairValue_01a0582c-fcd3-7924-94b2-3d0128623b8e" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofExposureinCommoditiesGrainsandOthersDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofExposureinCommoditiesGrainsandOthersDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofExposureinCommoditiesGrainsandOthersDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7758-b7f3-72a6e2e3ea14" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7dc0-af02-ef0eb3e6ba60" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7758-b7f3-72a6e2e3ea14" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7dc0-af02-ef0eb3e6ba60" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-722e-b655-1c5b099fc3ed" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7dc0-af02-ef0eb3e6ba60" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-722e-b655-1c5b099fc3ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-722e-b655-1c5b099fc3ed_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-722e-b655-1c5b099fc3ed" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-722e-b655-1c5b099fc3ed_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7aa6-b355-1317aa02d110" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesAxis_01a0582c-fcd3-722e-b655-1c5b099fc3ed" xlink:to="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7aa6-b355-1317aa02d110" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_GrainMember_01a0582c-fcd3-71aa-8db2-53a696f41a73" xlink:href="jbsay-20260930.xsd#jbsay_GrainMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CategoriesOfRelatedPartiesDomain_01a0582c-fcd3-7aa6-b355-1317aa02d110" xlink:to="loc_jbsay_GrainMember_01a0582c-fcd3-71aa-8db2-53a696f41a73" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7fe1-aa01-d5a22f09b39a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractsExposureScenarioDetailsTable_01a0582c-fcd3-7dc0-af02-ef0eb3e6ba60" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7fe1-aa01-d5a22f09b39a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fe1-aa01-d5a22f09b39a_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7fe1-aa01-d5a22f09b39a" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-7fe1-aa01-d5a22f09b39a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialAssetsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsAxis_01a0582c-fcd3-7fe1-aa01-d5a22f09b39a" xlink:to="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PurchaseOrdersMember_01a0582c-fcd3-7b7c-b44d-341efe434861" xlink:href="jbsay-20260930.xsd#jbsay_PurchaseOrdersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_PurchaseOrdersMember_01a0582c-fcd3-7b7c-b44d-341efe434861" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OperatingSubtotalMember_01a0582c-fcd3-7afd-9a45-cd97ca318e71" xlink:href="jbsay-20260930.xsd#jbsay_OperatingSubtotalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_OperatingSubtotalMember_01a0582c-fcd3-7afd-9a45-cd97ca318e71" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FutureB3Member_01a0582c-fcd3-70ce-809e-1cdca29d3b16" xlink:href="jbsay-20260930.xsd#jbsay_FutureB3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_FutureB3Member_01a0582c-fcd3-70ce-809e-1cdca29d3b16" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FutureCMEMember_01a0582c-fcd3-7bc6-be32-55803c2a6f61" xlink:href="jbsay-20260930.xsd#jbsay_FutureCMEMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_FutureCMEMember_01a0582c-fcd3-7bc6-be32-55803c2a6f61" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DeliverableForwardsMember_01a0582c-fcd3-7e19-b04c-def99a8688ac" xlink:href="jbsay-20260930.xsd#jbsay_DeliverableForwardsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_DeliverableForwardsMember_01a0582c-fcd3-7e19-b04c-def99a8688ac" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonDeliverableForwardsMember_01a0582c-fcd3-7918-92cf-673a3d4e75fc" xlink:href="jbsay-20260930.xsd#jbsay_NonDeliverableForwardsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_NonDeliverableForwardsMember_01a0582c-fcd3-7918-92cf-673a3d4e75fc" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_DerivativesSubtotalMember_01a0582c-fcd3-7b57-afe5-986a6e50966e" xlink:href="jbsay-20260930.xsd#jbsay_DerivativesSubtotalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialAssetsDomain_01a0582c-fcd3-75ff-b338-ab7b874f2694" xlink:to="loc_jbsay_DerivativesSubtotalMember_01a0582c-fcd3-7b57-afe5-986a6e50966e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NetExposureValue_01a0582c-fcd3-7165-9305-32eba2a5374d" xlink:href="jbsay-20260930.xsd#jbsay_NetExposureValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementAndFinancialInstrumentsScheduleOfContractsExposureScenarioDetailsLineItems_01a0582c-fcd3-7758-b7f3-72a6e2e3ea14" xlink:to="loc_jbsay_NetExposureValue_01a0582c-fcd3-7165-9305-32eba2a5374d" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#RiskmanagementandfinancialinstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/RiskmanagementandfinancialinstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsTable_01a0582c-fcd3-7788-b223-be1bc1c81721" xlink:href="jbsay-20260930.xsd#jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsTable_01a0582c-fcd3-7788-b223-be1bc1c81721" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityAxis_01a0582c-fcd3-7460-bd9c-3e8543f907b0" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MaturityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsTable_01a0582c-fcd3-7788-b223-be1bc1c81721" xlink:to="loc_ifrs-full_MaturityAxis_01a0582c-fcd3-7460-bd9c-3e8543f907b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7460-bd9c-3e8543f907b0_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_MaturityAxis_01a0582c-fcd3-7460-bd9c-3e8543f907b0" xlink:to="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7460-bd9c-3e8543f907b0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7a66-a084-bd65551fc586" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_MaturityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_MaturityAxis_01a0582c-fcd3-7460-bd9c-3e8543f907b0" xlink:to="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7a66-a084-bd65551fc586" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NotLaterThanOneYearMember_01a0582c-fcd3-7ab4-a602-fde74f72741c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NotLaterThanOneYearMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7a66-a084-bd65551fc586" xlink:to="loc_ifrs-full_NotLaterThanOneYearMember_01a0582c-fcd3-7ab4-a602-fde74f72741c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanOneYearAndNotLaterThanThreeYearsMember_01a0582c-fcd3-78d6-96ea-0dd5d20e975a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanOneYearAndNotLaterThanThreeYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7a66-a084-bd65551fc586" xlink:to="loc_ifrs-full_LaterThanOneYearAndNotLaterThanThreeYearsMember_01a0582c-fcd3-78d6-96ea-0dd5d20e975a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanFourYearsAndNotLaterThanFiveYearsMember_01a0582c-fcd3-7a5a-9aac-7239dd8ea006" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanFourYearsAndNotLaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7a66-a084-bd65551fc586" xlink:to="loc_ifrs-full_LaterThanFourYearsAndNotLaterThanFiveYearsMember_01a0582c-fcd3-7a5a-9aac-7239dd8ea006" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LaterThanFiveYearsMember_01a0582c-fcd3-7892-9ce0-998bb8e084a1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LaterThanFiveYearsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_MaturityDomain_01a0582c-fcd3-7a66-a084-bd65551fc586" xlink:to="loc_ifrs-full_LaterThanFiveYearsMember_01a0582c-fcd3-7892-9ce0-998bb8e084a1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_01a0582c-fcd3-7951-b67c-737a8f5c9200" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsTable_01a0582c-fcd3-7788-b223-be1bc1c81721" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_01a0582c-fcd3-7951-b67c-737a8f5c9200" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_01a0582c-fcd3-7951-b67c-737a8f5c9200_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_01a0582c-fcd3-7951-b67c-737a8f5c9200" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_01a0582c-fcd3-7951-b67c-737a8f5c9200_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_01a0582c-fcd3-706f-a859-b96b4a293b42" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ClassesOfFinancialLiabilitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesAxis_01a0582c-fcd3-7951-b67c-737a8f5c9200" xlink:to="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_01a0582c-fcd3-706f-a859-b96b4a293b42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_FinancialLiabilitiesCategoryMember_01a0582c-fcd3-762f-8a03-6b1ab9aa0a21" xlink:href="jbsay-20260930.xsd#jbsay_FinancialLiabilitiesCategoryMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ClassesOfFinancialLiabilitiesDomain_01a0582c-fcd3-706f-a859-b96b4a293b42" xlink:to="loc_jbsay_FinancialLiabilitiesCategoryMember_01a0582c-fcd3-762f-8a03-6b1ab9aa0a21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TradeAndOtherPayables_01a0582c-fcd3-7e52-961d-d750fa7e026b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherPayables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_ifrs-full_TradeAndOtherPayables_01a0582c-fcd3-7e52-961d-d750fa7e026b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LoansReceived_01a0582c-fcd3-79c4-b9c2-0dd6758dc55b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LoansReceived"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_ifrs-full_LoansReceived_01a0582c-fcd3-79c4-b9c2-0dd6758dc55b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherEquityInterest_01a0582c-fcd3-791e-8d5c-b6f03e82a6d5" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherEquityInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_ifrs-full_OtherEquityInterest_01a0582c-fcd3-791e-8d5c-b6f03e82a6d5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DerivativeFinancialLiabilities_01a0582c-fcd3-7e8b-af41-b8781ffcc4fc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DerivativeFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_ifrs-full_DerivativeFinancialLiabilities_01a0582c-fcd3-7e8b-af41-b8781ffcc4fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_PaymentsOfLeases_01a0582c-fcd3-79fa-b341-a9cb4902dfa1" xlink:href="jbsay-20260930.xsd#jbsay_PaymentsOfLeases"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_jbsay_PaymentsOfLeases_01a0582c-fcd3-79fa-b341-a9cb4902dfa1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CommoditiesForwardPurchaseContracts_01a0582c-fcd3-7111-ae5c-b79e7201c3fd" xlink:href="jbsay-20260930.xsd#jbsay_CommoditiesForwardPurchaseContracts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_jbsay_RiskManagementandFinancialInstrumentsScheduleofContractualObligationAmountsfromFinancialLiabilitiesDetailsLineItems_01a0582c-fcd3-7691-8bd0-a909baa43325" xlink:to="loc_jbsay_CommoditiesForwardPurchaseContracts_01a0582c-fcd3-7111-ae5c-b79e7201c3fd" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/SupplementalfinancialinformationScheduleofConsolidatedStatementsofFinancialPositionDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#SupplementalfinancialinformationScheduleofConsolidatedStatementsofFinancialPositionDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/SupplementalfinancialinformationScheduleofConsolidatedStatementsofFinancialPositionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7766-9350-b67ad6dbbe4e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7766-9350-b67ad6dbbe4e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ca6-bee0-ec012a802f07" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7766-9350-b67ad6dbbe4e" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ca6-bee0-ec012a802f07" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7ca6-bee0-ec012a802f07_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ca6-bee0-ec012a802f07" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7ca6-bee0-ec012a802f07_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-70a4-abb2-3e1fb6da985b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ca6-bee0-ec012a802f07" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-70a4-abb2-3e1fb6da985b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RestrictedSubsidiariesMember_01a0582c-fcd4-765f-98cc-1a9a63e54e2b" xlink:href="jbsay-20260930.xsd#jbsay_RestrictedSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-70a4-abb2-3e1fb6da985b" xlink:to="loc_jbsay_RestrictedSubsidiariesMember_01a0582c-fcd4-765f-98cc-1a9a63e54e2b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UnrestrictedSubsidiariesMember_01a0582c-fcd4-7186-973d-dca6e572241b" xlink:href="jbsay-20260930.xsd#jbsay_UnrestrictedSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-70a4-abb2-3e1fb6da985b" xlink:to="loc_jbsay_UnrestrictedSubsidiariesMember_01a0582c-fcd4-7186-973d-dca6e572241b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-770d-9fd4-c7ede9100600" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7766-9350-b67ad6dbbe4e" xlink:to="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-770d-9fd4-c7ede9100600" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-770d-9fd4-c7ede9100600_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-770d-9fd4-c7ede9100600" xlink:to="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-770d-9fd4-c7ede9100600_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7bce-9af2-b358523d87b7" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-770d-9fd4-c7ede9100600" xlink:to="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7bce-9af2-b358523d87b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ReportableLegalEntitiesMember_01a0582c-fcd4-7657-848c-f50211f135ce" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ReportableLegalEntitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7bce-9af2-b358523d87b7" xlink:to="loc_srt_ReportableLegalEntitiesMember_01a0582c-fcd4-7657-848c-f50211f135ce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationEliminationsMember_01a0582c-fcd4-74f7-b999-2fa30355ccb5" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationEliminationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7bce-9af2-b358523d87b7" xlink:to="loc_srt_ConsolidationEliminationsMember_01a0582c-fcd4-74f7-b999-2fa30355ccb5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentAssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CashAndCashEquivalents_01a0582c-fcd4-79a0-b796-19d3d1e28e1b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CashAndCashEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_CashAndCashEquivalents_01a0582c-fcd4-79a0-b796-19d3d1e28e1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentMarginCash_01a0582c-fcd4-7efa-97dc-0b54ee944e90" xlink:href="jbsay-20260930.xsd#jbsay_CurrentMarginCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_jbsay_CurrentMarginCash_01a0582c-fcd4-7efa-97dc-0b54ee944e90" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentTradeReceivables_01a0582c-fcd4-7394-8c24-bf6ad8f77ac3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTradeReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_CurrentTradeReceivables_01a0582c-fcd4-7394-8c24-bf6ad8f77ac3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentDividendsReceivable_01a189cd-d618-7e46-b806-b8217243a023" xlink:href="jbsay-20260930.xsd#jbsay_CurrentDividendsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_jbsay_CurrentDividendsReceivable_01a189cd-d618-7e46-b806-b8217243a023" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Inventories_01a0582c-fcd4-7b9b-871d-275b41869c94" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Inventories"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_Inventories_01a0582c-fcd4-7b9b-871d-275b41869c94" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentBiologicalAssets_01a0582c-fcd4-7fba-9268-340959bd06c1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentBiologicalAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_CurrentBiologicalAssets_01a0582c-fcd4-7fba-9268-340959bd06c1" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentRecoverableTaxes_01a0582c-fcd4-75b5-99e5-4e0c95a08509" xlink:href="jbsay-20260930.xsd#jbsay_CurrentRecoverableTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_jbsay_CurrentRecoverableTaxes_01a0582c-fcd4-75b5-99e5-4e0c95a08509" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentDerivativeFinancialAssets_01a0582c-fcd4-7117-afa7-895b02cbca1b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentDerivativeFinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_CurrentDerivativeFinancialAssets_01a0582c-fcd4-7117-afa7-895b02cbca1b" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherCurrentAssets_01a0582c-fcd4-7c59-a04f-3e5e6d67119a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_OtherCurrentAssets_01a0582c-fcd4-7c59-a04f-3e5e6d67119a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentAssets_01a0582c-fcd4-79b8-a068-57ebaa743787" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentAssetsAbstract_01a0582c-fcd4-740d-b423-a835b03b887c" xlink:to="loc_ifrs-full_CurrentAssets_01a0582c-fcd4-79b8-a068-57ebaa743787" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentAssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonCurrentInvestment_01a0582c-fcd4-7ed4-980c-1ee263d449c3" xlink:href="jbsay-20260930.xsd#jbsay_NonCurrentInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_jbsay_NonCurrentInvestment_01a0582c-fcd4-7ed4-980c-1ee263d449c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonCurrentRecoverableTaxes_01a0582c-fcd4-7d89-b740-5a390959b5bf" xlink:href="jbsay-20260930.xsd#jbsay_NonCurrentRecoverableTaxes"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_jbsay_NonCurrentRecoverableTaxes_01a0582c-fcd4-7d89-b740-5a390959b5bf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentBiologicalAssets_01a0582c-fcd4-7cfc-9ca8-0ddda8847ccf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentBiologicalAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_NoncurrentBiologicalAssets_01a0582c-fcd4-7cfc-9ca8-0ddda8847ccf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentReceivablesDueFromRelatedParties_01a0582c-fcd4-7f92-8e52-02b73b79a1cb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentReceivablesDueFromRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_NoncurrentReceivablesDueFromRelatedParties_01a0582c-fcd4-7f92-8e52-02b73b79a1cb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxAssets_01a0582c-fcd4-7205-966a-f934710977b2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_DeferredTaxAssets_01a0582c-fcd4-7205-966a-f934710977b2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherNoncurrentAssets_01a0582c-fcd4-752a-9828-46d56a7d8979" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherNoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_OtherNoncurrentAssets_01a0582c-fcd4-752a-9828-46d56a7d8979" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NoncurrentAssetsAggregateSubtotal_01a1a80b-12c0-7590-b904-b078cea12d7d" xlink:href="jbsay-20260930.xsd#jbsay_NoncurrentAssetsAggregateSubtotal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_jbsay_NoncurrentAssetsAggregateSubtotal_01a1a80b-12c0-7590-b904-b078cea12d7d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_InvestmentAccountedForUsingEquityMethod_01a0582c-fcd4-7424-8fc3-45026039105d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_InvestmentAccountedForUsingEquityMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_InvestmentAccountedForUsingEquityMethod_01a0582c-fcd4-7424-8fc3-45026039105d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_PropertyPlantAndEquipment_01a0582c-fcd4-7971-a163-4315de14e59a" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_PropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_PropertyPlantAndEquipment_01a0582c-fcd4-7971-a163-4315de14e59a" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_RightofuseAssets_01a0582c-fcd4-79ed-a999-3df7bab81bb3" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RightofuseAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_RightofuseAssets_01a0582c-fcd4-79ed-a999-3df7bab81bb3" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IntangibleAssetsOtherThanGoodwill_01a0582c-fcd4-7cab-bd46-70d4be2f1986" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IntangibleAssetsOtherThanGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_IntangibleAssetsOtherThanGoodwill_01a0582c-fcd4-7cab-bd46-70d4be2f1986" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Goodwill_01a0582c-fcd4-73fc-9868-86f157461990" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_Goodwill_01a0582c-fcd4-73fc-9868-86f157461990" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentAssets_01a0582c-fcd4-75bf-8a6e-ddb69bdc5383" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentAssetsAbstract_01a0582c-fcd4-78b1-8659-166096166582" xlink:to="loc_ifrs-full_NoncurrentAssets_01a0582c-fcd4-75bf-8a6e-ddb69bdc5383" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Assets_01a0582c-fcd4-767f-b07e-18b6dd040138" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_Assets_01a0582c-fcd4-767f-b07e-18b6dd040138" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers_01a0582c-fcd4-7f85-83b4-168eea9eb548" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_TradeAndOtherCurrentPayablesToTradeSuppliers_01a0582c-fcd4-7f85-83b4-168eea9eb548" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_CurrentSupplyChainFinance_01a0582c-fcd4-78ef-a353-93d4213632e2" xlink:href="jbsay-20260930.xsd#jbsay_CurrentSupplyChainFinance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_jbsay_CurrentSupplyChainFinance_01a0582c-fcd4-78ef-a353-93d4213632e2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ShorttermBorrowings_01a0582c-fcd4-7f4d-b419-0c33bdf912d2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ShorttermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_ShorttermBorrowings_01a0582c-fcd4-7f4d-b419-0c33bdf912d2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentTaxLiabilitiesCurrent_01a0582c-fcd4-7ed4-a059-01ede864fe30" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTaxLiabilitiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_CurrentTaxLiabilitiesCurrent_01a0582c-fcd4-7ed4-a059-01ede864fe30" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherCurrentPayables_01a0582c-fcd4-7858-95fd-2e4b2b488f65" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentPayables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_OtherCurrentPayables_01a0582c-fcd4-7858-95fd-2e4b2b488f65" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentProvisionsForEmployeeBenefits_01a0582c-fcd4-759c-8d71-f6df42f1fac2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentProvisionsForEmployeeBenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_CurrentProvisionsForEmployeeBenefits_01a0582c-fcd4-759c-8d71-f6df42f1fac2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentLeaseLiabilities_01a0582c-fcd4-7d65-a59f-76a25e9eef95" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentLeaseLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_CurrentLeaseLiabilities_01a0582c-fcd4-7d65-a59f-76a25e9eef95" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DividendPayables_01a0582c-fcd4-7d2b-a78b-74970114b188" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DividendPayables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_DividendPayables_01a0582c-fcd4-7d2b-a78b-74970114b188" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherShorttermProvisions_01a0582c-fcd4-70ec-ae15-d1b6a82deaad" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherShorttermProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_OtherShorttermProvisions_01a0582c-fcd4-70ec-ae15-d1b6a82deaad" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentDerivativeFinancialLiabilities_01a0582c-fcd4-73c8-a05c-4a79f641da78" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentDerivativeFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_CurrentDerivativeFinancialLiabilities_01a0582c-fcd4-73c8-a05c-4a79f641da78" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherCurrentLiabilities_01a0582c-fcd4-7766-9ce0-b12578d560cc" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherCurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_OtherCurrentLiabilities_01a0582c-fcd4-7766-9ce0-b12578d560cc" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentLiabilities_01a0582c-fcd4-7817-abd1-8eeaab40e0b6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_CurrentLiabilitiesAbstract_01a0582c-fcd4-7bba-8da6-792b7bbe7ecb" xlink:to="loc_ifrs-full_CurrentLiabilities_01a0582c-fcd4-7817-abd1-8eeaab40e0b6" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_LongtermBorrowings_01a0582c-fcd4-7309-8f61-860e43b631cf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LongtermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_LongtermBorrowings_01a0582c-fcd4-7309-8f61-860e43b631cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentTaxLiabilitiesNoncurrent_01a0582c-fcd4-7a41-97a2-b186ca36942e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTaxLiabilitiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_CurrentTaxLiabilitiesNoncurrent_01a0582c-fcd4-7a41-97a2-b186ca36942e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_NonCurrentProvisionsForEmployeeBenefitsAdjusted_01a1b1ec-0ddf-709e-86f6-2eb3533c93fc" xlink:href="jbsay-20260930.xsd#jbsay_NonCurrentProvisionsForEmployeeBenefitsAdjusted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_jbsay_NonCurrentProvisionsForEmployeeBenefitsAdjusted_01a1b1ec-0ddf-709e-86f6-2eb3533c93fc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentProvisionsForEmployeeBenefits_01a0582c-fcd4-7391-ad66-1d67f3fc8de7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentProvisionsForEmployeeBenefits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_NoncurrentProvisionsForEmployeeBenefits_01a0582c-fcd4-7391-ad66-1d67f3fc8de7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentLeaseLiabilities_01a0582c-fcd4-7bcf-9a65-10edcfb0fcd6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentLeaseLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_NoncurrentLeaseLiabilities_01a0582c-fcd4-7bcf-9a65-10edcfb0fcd6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxLiabilities_01a0582c-fcd4-7445-8c18-e7e2be8952ae" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_DeferredTaxLiabilities_01a0582c-fcd4-7445-8c18-e7e2be8952ae" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherLongtermProvisions_01a0582c-fcd4-79ca-9e99-f92d9ab1a253" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherLongtermProvisions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_OtherLongtermProvisions_01a0582c-fcd4-79ca-9e99-f92d9ab1a253" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentPayablesToRelatedParties_01a0582c-fcd4-7a33-b515-a1bbc3f8ca9f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentPayablesToRelatedParties"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_NoncurrentPayablesToRelatedParties_01a0582c-fcd4-7a33-b515-a1bbc3f8ca9f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentDerivativeFinancialLiabilities_01a0582c-fcd4-753f-87a8-ac16fbc9d829" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentDerivativeFinancialLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_NoncurrentDerivativeFinancialLiabilities_01a0582c-fcd4-753f-87a8-ac16fbc9d829" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherNoncurrentLiabilities_01a0582c-fcd4-7ad3-95c0-ae7f25a7b8bf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherNoncurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_OtherNoncurrentLiabilities_01a0582c-fcd4-7ad3-95c0-ae7f25a7b8bf" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncurrentLiabilities_01a0582c-fcd4-7d6d-8b45-296e3446c5a7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncurrentLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_NoncurrentLiabilitiesAbstract_01a0582c-fcd4-7007-8213-1eed67b5afe9" xlink:to="loc_ifrs-full_NoncurrentLiabilities_01a0582c-fcd4-7d6d-8b45-296e3446c5a7" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EquityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IssuedCapital_01a0582c-fcd4-7c0e-b8e6-2aca730d990f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IssuedCapital"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:to="loc_ifrs-full_IssuedCapital_01a0582c-fcd4-7c0e-b8e6-2aca730d990f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_StatutoryReserveAdjusted_01a1b1ea-c9bf-72c8-a998-0fe86021e719" xlink:href="jbsay-20260930.xsd#jbsay_StatutoryReserveAdjusted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:to="loc_jbsay_StatutoryReserveAdjusted_01a1b1ea-c9bf-72c8-a998-0fe86021e719" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UndistributedResults_01a0582c-fcd4-7c80-8fc8-3e5d3d0e1ccd" xlink:href="jbsay-20260930.xsd#jbsay_UndistributedResults"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:to="loc_jbsay_UndistributedResults_01a0582c-fcd4-7c80-8fc8-3e5d3d0e1ccd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_AccumulatedOtherComprehensiveIncomeLoss_01a1a62a-0f7c-7d6f-ab9e-1b67487292b7" xlink:href="jbsay-20260930.xsd#jbsay_AccumulatedOtherComprehensiveIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:to="loc_jbsay_AccumulatedOtherComprehensiveIncomeLoss_01a1a62a-0f7c-7d6f-ab9e-1b67487292b7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EquityAttributableToOwnersOfParent_01a0582c-fcd4-741d-aa39-90846364d192" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EquityAttributableToOwnersOfParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_EquityAbstract_01a0582c-fcd4-7906-9040-8223f02c3791" xlink:to="loc_ifrs-full_EquityAttributableToOwnersOfParent_01a0582c-fcd4-741d-aa39-90846364d192" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_NoncontrollingInterests_01a0582c-fcd4-770d-8494-a839c71cddaa" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NoncontrollingInterests"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_NoncontrollingInterests_01a0582c-fcd4-770d-8494-a839c71cddaa" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Equity_01a0582c-fcd4-785b-801a-c2fb59366036" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Equity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_Equity_01a0582c-fcd4-785b-801a-c2fb59366036" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_EquityAndLiabilities_01a0582c-fcd4-70e2-9958-68965528bf1f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_EquityAndLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-709a-9a89-060a2c1c2f1e" xlink:to="loc_ifrs-full_EquityAndLiabilities_01a0582c-fcd4-70e2-9958-68965528bf1f" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/SupplementalfinancialinformationScheduleofConsolidatedStatementsofIncomeDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#SupplementalfinancialinformationScheduleofConsolidatedStatementsofIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/SupplementalfinancialinformationScheduleofConsolidatedStatementsofIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7312-9462-76e5bf0d214e" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7312-9462-76e5bf0d214e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-784a-aa0b-905d9c0e4282" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7312-9462-76e5bf0d214e" xlink:to="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-784a-aa0b-905d9c0e4282" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-784a-aa0b-905d9c0e4282_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-784a-aa0b-905d9c0e4282" xlink:to="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-784a-aa0b-905d9c0e4282_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7d7c-aecd-8dddfeff6f15" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-784a-aa0b-905d9c0e4282" xlink:to="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7d7c-aecd-8dddfeff6f15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ReportableLegalEntitiesMember_01a0582c-fcd4-7e33-a561-8bd84e69b837" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ReportableLegalEntitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7d7c-aecd-8dddfeff6f15" xlink:to="loc_srt_ReportableLegalEntitiesMember_01a0582c-fcd4-7e33-a561-8bd84e69b837" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationEliminationsMember_01a0582c-fcd4-73ef-be59-eac713b698c9" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationEliminationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7d7c-aecd-8dddfeff6f15" xlink:to="loc_srt_ConsolidationEliminationsMember_01a0582c-fcd4-73ef-be59-eac713b698c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7d91-a1cb-c2488f2b9619" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7312-9462-76e5bf0d214e" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7d91-a1cb-c2488f2b9619" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7d91-a1cb-c2488f2b9619_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7d91-a1cb-c2488f2b9619" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7d91-a1cb-c2488f2b9619_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7502-bd48-d6d663eae31f" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7d91-a1cb-c2488f2b9619" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7502-bd48-d6d663eae31f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RestrictedSubsidiariesMember_01a0582c-fcd4-73e4-a494-a6252456e7c9" xlink:href="jbsay-20260930.xsd#jbsay_RestrictedSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7502-bd48-d6d663eae31f" xlink:to="loc_jbsay_RestrictedSubsidiariesMember_01a0582c-fcd4-73e4-a494-a6252456e7c9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UnrestrictedSubsidiariesMember_01a0582c-fcd4-7802-aa1f-a31474587edb" xlink:href="jbsay-20260930.xsd#jbsay_UnrestrictedSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7502-bd48-d6d663eae31f" xlink:to="loc_jbsay_UnrestrictedSubsidiariesMember_01a0582c-fcd4-7802-aa1f-a31474587edb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_Revenue_01a0582c-fcd4-7ead-a570-a2bde8c2162b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Revenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_Revenue_01a0582c-fcd4-7ead-a570-a2bde8c2162b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CostOfSales_01a0582c-fcd4-7577-a0e2-f055474a6121" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CostOfSales"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_CostOfSales_01a0582c-fcd4-7577-a0e2-f055474a6121" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GrossProfit_01a0582c-fcd4-7053-b5e1-fb123ca44cbf" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_GrossProfit_01a0582c-fcd4-7053-b5e1-fb123ca44cbf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GeneralAndAdministrativeExpense_01a0582c-fcd4-74ea-b13f-46e110e7d7dd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_GeneralAndAdministrativeExpense_01a0582c-fcd4-74ea-b13f-46e110e7d7dd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_SellingExpense_01a0582c-fcd4-7117-b6ba-bd36ba480558" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SellingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_SellingExpense_01a0582c-fcd4-7117-b6ba-bd36ba480558" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherIncome_01a0582c-fcd4-7968-a3ca-12aa048c575d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_OtherIncome_01a0582c-fcd4-7968-a3ca-12aa048c575d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherExpenseByNature_01a0582c-fcd4-7d49-87cd-602247d4c183" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherExpenseByNature"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_OtherExpenseByNature_01a0582c-fcd4-7d49-87cd-602247d4c183" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OperatingExpense_01a0582c-fcd4-7368-9fc5-ee6c2b0748fd" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OperatingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_OperatingExpense_01a0582c-fcd4-7368-9fc5-ee6c2b0748fd" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossFromOperatingActivities_01a0582c-fcd4-7581-abb0-54066e68b845" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossFromOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_ProfitLossFromOperatingActivities_01a0582c-fcd4-7581-abb0-54066e68b845" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinanceIncome_01a0582c-fcd4-70b6-8253-95f110fb8934" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_FinanceIncome_01a0582c-fcd4-70b6-8253-95f110fb8934" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinanceCosts_01a0582c-fcd4-7e2d-99ba-7576f3823067" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_FinanceCosts_01a0582c-fcd4-7e2d-99ba-7576f3823067" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_FinanceIncomeCost_01a0582c-fcd4-7447-a9cc-6585855aa7e7" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceIncomeCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_FinanceIncomeCost_01a0582c-fcd4-7447-a9cc-6585855aa7e7" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ShareOfProfitLossOfEquityAccountedInvesteesNetOfTax_01a0582c-fcd4-7a7b-adb6-fc2a61601f99" xlink:href="jbsay-20260930.xsd#jbsay_ShareOfProfitLossOfEquityAccountedInvesteesNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_jbsay_ShareOfProfitLossOfEquityAccountedInvesteesNetOfTax_01a0582c-fcd4-7a7b-adb6-fc2a61601f99" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossBeforeTax_01a0582c-fcd4-71f2-a482-71f1cdefd77d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_ProfitLossBeforeTax_01a0582c-fcd4-71f2-a482-71f1cdefd77d" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_CurrentTaxExpenseIncome_01a0582c-fcd4-72e7-9866-c47bc0a00b67" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentTaxExpenseIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_CurrentTaxExpenseIncome_01a0582c-fcd4-72e7-9866-c47bc0a00b67" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DeferredTaxExpenseIncome_01a0582c-fcd4-7064-85ab-ffd891e14958" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DeferredTaxExpenseIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_DeferredTaxExpenseIncome_01a0582c-fcd4-7064-85ab-ffd891e14958" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncomeTaxExpenseContinuingOperations_01a0582c-fcd4-70ba-bc32-f73c3d050808" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncomeTaxExpenseContinuingOperations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_IncomeTaxExpenseContinuingOperations_01a0582c-fcd4-70ba-bc32-f73c3d050808" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_01a0582c-fcd4-7547-a758-1b873a0c5ca6" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_ProfitLoss_01a0582c-fcd4-7547-a758-1b873a0c5ca6" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossAttributableToAbstract_01a0582c-fcd4-7b1d-9b15-39f3b6c4be65" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossAttributableToAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-7f05-8e49-29edbb4a81fa" xlink:to="loc_ifrs-full_ProfitLossAttributableToAbstract_01a0582c-fcd4-7b1d-9b15-39f3b6c4be65" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossAttributableToOwnersOfParent_01a0582c-fcd4-7000-b03e-72e475e9276b" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossAttributableToOwnersOfParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProfitLossAttributableToAbstract_01a0582c-fcd4-7b1d-9b15-39f3b6c4be65" xlink:to="loc_ifrs-full_ProfitLossAttributableToOwnersOfParent_01a0582c-fcd4-7000-b03e-72e475e9276b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLossAttributableToNoncontrollingInterests_01a0582c-fcd4-70b8-bccf-76c67d41a8a4" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLossAttributableToNoncontrollingInterests"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ProfitLossAttributableToAbstract_01a0582c-fcd4-7b1d-9b15-39f3b6c4be65" xlink:to="loc_ifrs-full_ProfitLossAttributableToNoncontrollingInterests_01a0582c-fcd4-70b8-bccf-76c67d41a8a4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_01a0582c-fcd4-73cf-8bed-7b3e4245f56d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.jbssa.com/role/SupplementalfinancialinformationScheduleofComprehensiveIncomeDetails" xlink:type="simple" xlink:href="jbsay-20260930.xsd#SupplementalfinancialinformationScheduleofComprehensiveIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.jbssa.com/role/SupplementalfinancialinformationScheduleofComprehensiveIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7d24-a25c-3ca7a1bd5da1" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7d24-a25c-3ca7a1bd5da1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-7bfe-b0a6-e276e07da434" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7d24-a25c-3ca7a1bd5da1" xlink:to="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-7bfe-b0a6-e276e07da434" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7bfe-b0a6-e276e07da434_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-7bfe-b0a6-e276e07da434" xlink:to="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7bfe-b0a6-e276e07da434_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7286-859a-0aef5c56e8e3" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_01a0582c-fcd4-7bfe-b0a6-e276e07da434" xlink:to="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7286-859a-0aef5c56e8e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ReportableLegalEntitiesMember_01a0582c-fcd4-74a8-b354-5943772aa830" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ReportableLegalEntitiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7286-859a-0aef5c56e8e3" xlink:to="loc_srt_ReportableLegalEntitiesMember_01a0582c-fcd4-74a8-b354-5943772aa830" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationEliminationsMember_01a0582c-fcd4-7c32-9345-04e73534b955" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_ConsolidationEliminationsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_01a0582c-fcd4-7286-859a-0aef5c56e8e3" xlink:to="loc_srt_ConsolidationEliminationsMember_01a0582c-fcd4-7c32-9345-04e73534b955" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ec3-8616-0e937c5528f8" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesTable_01a0582c-fcd4-7d24-a25c-3ca7a1bd5da1" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ec3-8616-0e937c5528f8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7ec3-8616-0e937c5528f8_default" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ec3-8616-0e937c5528f8" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-7ec3-8616-0e937c5528f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-74d4-b173-56eb988fc590" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ConsolidatedStructuredEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesAxis_01a0582c-fcd4-7ec3-8616-0e937c5528f8" xlink:to="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-74d4-b173-56eb988fc590" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_RestrictedSubsidiariesMember_01a0582c-fcd4-794d-b751-1b9c062d74b7" xlink:href="jbsay-20260930.xsd#jbsay_RestrictedSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-74d4-b173-56eb988fc590" xlink:to="loc_jbsay_RestrictedSubsidiariesMember_01a0582c-fcd4-794d-b751-1b9c062d74b7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_UnrestrictedSubsidiariesMember_01a0582c-fcd4-72bf-bb0d-997cf9cf74a6" xlink:href="jbsay-20260930.xsd#jbsay_UnrestrictedSubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ConsolidatedStructuredEntitiesDomain_01a0582c-fcd4-74d4-b173-56eb988fc590" xlink:to="loc_jbsay_UnrestrictedSubsidiariesMember_01a0582c-fcd4-72bf-bb0d-997cf9cf74a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ProfitLoss_01a0582c-fcd4-778c-9ab8-a13ad6500964" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_ProfitLoss_01a0582c-fcd4-778c-9ab8-a13ad6500964" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-70f0-af89-6d04e2c22418" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-70f0-af89-6d04e2c22418" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnExchangeDifferencesOnTranslationNetOfTax_01a0582c-fcd4-73a2-82d3-7496c9a5ee30" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnExchangeDifferencesOnTranslationNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-70f0-af89-6d04e2c22418" xlink:to="loc_ifrs-full_GainsLossesOnExchangeDifferencesOnTranslationNetOfTax_01a0582c-fcd4-73a2-82d3-7496c9a5ee30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_GainsLossesOnFinancialAssetsMeasuredAtFairValueThroughOtherComprehensiveIncomeNetOfTax_01a0582c-fcd4-7fd4-878f-d3c592ed53a0" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GainsLossesOnFinancialAssetsMeasuredAtFairValueThroughOtherComprehensiveIncomeNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-70f0-af89-6d04e2c22418" xlink:to="loc_ifrs-full_GainsLossesOnFinancialAssetsMeasuredAtFairValueThroughOtherComprehensiveIncomeNetOfTax_01a0582c-fcd4-7fd4-878f-d3c592ed53a0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_GainsLossesOnDeferredIncomeTaxOnLossCashFlowHedges_01a0582c-fcd4-79f4-8f82-a892ea01bd07" xlink:href="jbsay-20260930.xsd#jbsay_GainsLossesOnDeferredIncomeTaxOnLossCashFlowHedges"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-70f0-af89-6d04e2c22418" xlink:to="loc_jbsay_GainsLossesOnDeferredIncomeTaxOnLossCashFlowHedges_01a0582c-fcd4-79f4-8f82-a892ea01bd07" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_OtherFairValueAdjustmentThroughOtherComprehensiveIncome_01a0582c-fcd4-733a-872b-0674ec182e37" xlink:href="jbsay-20260930.xsd#jbsay_OtherFairValueAdjustmentThroughOtherComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-70f0-af89-6d04e2c22418" xlink:to="loc_jbsay_OtherFairValueAdjustmentThroughOtherComprehensiveIncome_01a0582c-fcd4-733a-872b-0674ec182e37" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillNotBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-7732-a269-f65cc195c061" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillNotBeReclassifiedToProfitOrLossNetOfTaxAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillNotBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-7732-a269-f65cc195c061" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherComprehensiveIncomeNetOfTaxGainsLossesOnRemeasurementsOfDefinedBenefitPlans_01a0582c-fcd4-7e3a-b1fd-cb25db965711" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherComprehensiveIncomeNetOfTaxGainsLossesOnRemeasurementsOfDefinedBenefitPlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillNotBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-7732-a269-f65cc195c061" xlink:to="loc_ifrs-full_OtherComprehensiveIncomeNetOfTaxGainsLossesOnRemeasurementsOfDefinedBenefitPlans_01a0582c-fcd4-7e3a-b1fd-cb25db965711" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_IncomeTaxRelatingToRemeasurementsOfDefinedBenefitPlansOfOtherComprehensiveIncome_01a0582c-fcd4-7070-b15e-09242713cd0d" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncomeTaxRelatingToRemeasurementsOfDefinedBenefitPlansOfOtherComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComponentsOfOtherComprehensiveIncomeThatWillNotBeReclassifiedToProfitOrLossNetOfTaxAbstract_01a0582c-fcd4-7732-a269-f65cc195c061" xlink:to="loc_ifrs-full_IncomeTaxRelatingToRemeasurementsOfDefinedBenefitPlansOfOtherComprehensiveIncome_01a0582c-fcd4-7070-b15e-09242713cd0d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_OtherComprehensiveIncome_01a0582c-fcd4-7ac6-b170-9a40110c77e2" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_OtherComprehensiveIncome_01a0582c-fcd4-7ac6-b170-9a40110c77e2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ComprehensiveIncomeBeforeSubsidiaries_01a0582c-fcd4-779d-bcc0-750641daa6b5" xlink:href="jbsay-20260930.xsd#jbsay_ComprehensiveIncomeBeforeSubsidiaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_jbsay_ComprehensiveIncomeBeforeSubsidiaries_01a0582c-fcd4-779d-bcc0-750641daa6b5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_jbsay_ComprehensiveIncomeIncomeOnSubsidiaries_01a0582c-fcd4-75a2-a90b-dfd889abab5a" xlink:href="jbsay-20260930.xsd#jbsay_ComprehensiveIncomeIncomeOnSubsidiaries"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_jbsay_ComprehensiveIncomeIncomeOnSubsidiaries_01a0582c-fcd4-75a2-a90b-dfd889abab5a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComprehensiveIncomeAttributableToAbstract_01a0582c-fcd4-7e9c-ba06-c3b173f65497" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncomeAttributableToAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_ComprehensiveIncomeAttributableToAbstract_01a0582c-fcd4-7e9c-ba06-c3b173f65497" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComprehensiveIncomeAttributableToOwnersOfParent_01a0582c-fcd4-7227-a966-0726022501f9" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncomeAttributableToOwnersOfParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComprehensiveIncomeAttributableToAbstract_01a0582c-fcd4-7e9c-ba06-c3b173f65497" xlink:to="loc_ifrs-full_ComprehensiveIncomeAttributableToOwnersOfParent_01a0582c-fcd4-7227-a966-0726022501f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComprehensiveIncomeAttributableToNoncontrollingInterests_01a0582c-fcd4-774f-960d-10f90cd5ae0c" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncomeAttributableToNoncontrollingInterests"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_ComprehensiveIncomeAttributableToAbstract_01a0582c-fcd4-7e9c-ba06-c3b173f65497" xlink:to="loc_ifrs-full_ComprehensiveIncomeAttributableToNoncontrollingInterests_01a0582c-fcd4-774f-960d-10f90cd5ae0c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_ifrs-full_ComprehensiveIncome_01a0582c-fcd4-7f8b-b320-12f9bfa04b41" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComprehensiveIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-full_DisclosureOfSignificantInvestmentsInSubsidiariesLineItems_01a0582c-fcd4-71c4-bb7f-35d373471cb5" xlink:to="loc_ifrs-full_ComprehensiveIncome_01a0582c-fcd4-7f8b-b320-12f9bfa04b41" xlink:type="arc" order="7"/>
  </link:definitionLink>
</link:linkbase>
