<?xml version="1.0" encoding="utf-8"?>
<!--XBRL document created with Toppan Merrill Bridge  11.5.1.0 -->
<!--Based on XBRL 2.1-->
<!--Created on: 11/5/2026 1:30:53 PM-->
<!--Modified on: 11/5/2026 1:30:53 PM-->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
  <link:roleRef roleURI="http://xbrl.sec.gov/spac/role/DilutionSourcesOnly" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#DilutionSourcesOnly" xlink:type="simple" />
  <link:roleRef roleURI="http://xbrl.sec.gov/spac/role/DirectAndIndirectMaterialInterestHoldersOnly" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#DirectAndIndirectMaterialInterestHoldersOnly" xlink:type="simple" />
  <link:roleRef roleURI="http://xbrl.sec.gov/spac/role/CompensationNaturesOnly" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#CompensationNaturesOnly" xlink:type="simple" />
  <link:roleRef roleURI="http://xbrl.sec.gov/spac/role/SponsorsAffiliateOrPromotersOnly" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#SponsorsAffiliatesOrPromotersOnly" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" />
  <link:arcroleRef arcroleURI="http://www.esma.europa.eu/xbrl/esef/arcrole/wider-narrower" xlink:type="simple" xlink:href="http://www.xbrl.org/lrr/arcrole/esma-arcrole-2018-11-21.xsd#wider-narrower" />
  <link:definitionLink xlink:role="http://xbrl.sec.gov/spac/role/SponsorsAffiliateOrPromotersOnly" xlink:type="extended" xlink:title="Sponsors, Affiliates, or Promoters">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:label="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:title="spac_AllSpacSponsorsAffiliatesOrPromotersMember" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_ToonAcquisitionLlcMember" xlink:label="KACQC_ToonAcquisitionLlcMember" xlink:title="KACQC_ToonAcquisitionLlcMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:to="KACQC_ToonAcquisitionLlcMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorsAffiliatesOrPromotersMember To KACQC_ToonAcquisitionLlcMember" order="1" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_DirectorsMember" xlink:label="KACQC_DirectorsMember" xlink:title="KACQC_DirectorsMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:to="KACQC_DirectorsMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorsAffiliatesOrPromotersMember To KACQC_DirectorsMember" order="5" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:label="KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember_1" xlink:title="KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember_1" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:to="KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember_1" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorsAffiliatesOrPromotersMember To KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember_1" order="6" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_AndyHeywardMember" xlink:label="KACQC_AndyHeywardMember_1" xlink:title="KACQC_AndyHeywardMember_1" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:to="KACQC_AndyHeywardMember_1" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorsAffiliatesOrPromotersMember To KACQC_AndyHeywardMember_1" order="2" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_KevinMccarthyMember" xlink:label="KACQC_KevinMccarthyMember_1" xlink:title="KACQC_KevinMccarthyMember_1" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:to="KACQC_KevinMccarthyMember_1" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorsAffiliatesOrPromotersMember To KACQC_KevinMccarthyMember_1" order="3" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_Dr.ChrysostomosLNikiasMember" xlink:label="KACQC_Dr.ChrysostomosLNikiasMember_1" xlink:title="KACQC_Dr.ChrysostomosLNikiasMember_1" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorsAffiliatesOrPromotersMember" xlink:to="KACQC_Dr.ChrysostomosLNikiasMember_1" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorsAffiliatesOrPromotersMember To KACQC_Dr.ChrysostomosLNikiasMember_1" order="4" priority="0" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/spac/role/CompensationNaturesOnly" xlink:type="extended" xlink:title="Compensation Natures">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#spac_AllCompensationNaturesMember" xlink:label="spac_AllCompensationNaturesMember" xlink:title="spac_AllCompensationNaturesMember" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_WorkingCapitalLoansMember" xlink:label="KACQC_WorkingCapitalLoansMember" xlink:title="KACQC_WorkingCapitalLoansMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllCompensationNaturesMember" xlink:to="KACQC_WorkingCapitalLoansMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllCompensationNaturesMember To KACQC_WorkingCapitalLoansMember" order="6" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_AnyOutOfPocketExpensesMember" xlink:label="KACQC_AnyOutOfPocketExpensesMember" xlink:title="KACQC_AnyOutOfPocketExpensesMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllCompensationNaturesMember" xlink:to="KACQC_AnyOutOfPocketExpensesMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllCompensationNaturesMember To KACQC_AnyOutOfPocketExpensesMember" order="7" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_RepaymentOfLoansMember" xlink:label="KACQC_RepaymentOfLoansMember" xlink:title="KACQC_RepaymentOfLoansMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllCompensationNaturesMember" xlink:to="KACQC_RepaymentOfLoansMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllCompensationNaturesMember To KACQC_RepaymentOfLoansMember" order="5" priority="0" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/spac/role/DirectAndIndirectMaterialInterestHoldersOnly" xlink:type="extended" xlink:title="Direct and Indirect Material Interest Holders">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember" xlink:label="spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember" xlink:title="spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_ToonAcquisitionLlcMember" xlink:label="KACQC_ToonAcquisitionLlcMember_1" xlink:title="KACQC_ToonAcquisitionLlcMember_1" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember" xlink:to="KACQC_ToonAcquisitionLlcMember_1" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember To KACQC_ToonAcquisitionLlcMember_1" order="1" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:label="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:title="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember" xlink:to="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember To KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" order="2" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_AndyHeywardMember" xlink:label="KACQC_AndyHeywardMember" xlink:title="KACQC_AndyHeywardMember" />
    <link:definitionArc xlink:type="arc" xlink:from="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:to="KACQC_AndyHeywardMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember To KACQC_AndyHeywardMember" order="1" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_BrianParisiMember" xlink:label="KACQC_BrianParisiMember" xlink:title="KACQC_BrianParisiMember" />
    <link:definitionArc xlink:type="arc" xlink:from="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:to="KACQC_BrianParisiMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember To KACQC_BrianParisiMember" order="2" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_MichaelJaffaMember" xlink:label="KACQC_MichaelJaffaMember" xlink:title="KACQC_MichaelJaffaMember" />
    <link:definitionArc xlink:type="arc" xlink:from="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:to="KACQC_MichaelJaffaMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember To KACQC_MichaelJaffaMember" order="3" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_KevinMccarthyMember" xlink:label="KACQC_KevinMccarthyMember" xlink:title="KACQC_KevinMccarthyMember" />
    <link:definitionArc xlink:type="arc" xlink:from="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:to="KACQC_KevinMccarthyMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember To KACQC_KevinMccarthyMember" order="4" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_Dr.ChrysostomosLNikiasMember" xlink:label="KACQC_Dr.ChrysostomosLNikiasMember" xlink:title="KACQC_Dr.ChrysostomosLNikiasMember" />
    <link:definitionArc xlink:type="arc" xlink:from="KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:to="KACQC_Dr.ChrysostomosLNikiasMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:KACQC_AllOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember To KACQC_Dr.ChrysostomosLNikiasMember" order="5" priority="0" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:label="KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" xlink:title="KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember" xlink:to="KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllSpacSponsorDirectAndIndirectMaterialInterestHoldersMember To KACQC_ToonAcquisitionLlcOfficersDirectorsOrAdvisorsRespectiveAffiliatesMember" order="3" priority="0" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/spac/role/DilutionSourcesOnly" xlink:type="extended" xlink:title="Dilution Sources">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/spac/2026/spac-2026.xsd#spac_AllDilutionSourcesMember" xlink:label="spac_AllDilutionSourcesMember" xlink:title="spac_AllDilutionSourcesMember" />
    <link:loc xlink:type="locator" xlink:href="tmb-20261105.xsd#KACQC_OfferingCostsAccruedOrPaidInAdvanceMember" xlink:label="KACQC_OfferingCostsAccruedOrPaidInAdvanceMember" xlink:title="KACQC_OfferingCostsAccruedOrPaidInAdvanceMember" />
    <link:definitionArc xlink:type="arc" xlink:from="spac_AllDilutionSourcesMember" xlink:to="KACQC_OfferingCostsAccruedOrPaidInAdvanceMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:spac_AllDilutionSourcesMember To KACQC_OfferingCostsAccruedOrPaidInAdvanceMember" order="11" priority="0" />
  </link:definitionLink>
</link:linkbase>
