<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:01a17e83-ae32-74e4-8c36-322b913c2430,g:30883fda-9eed-4098-b561-b26b848c908c-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://q2ebanking.com/role/CoverPage" xlink:type="simple" xlink:href="qtwo-20261028.xsd#CoverPage"/>
  <link:definitionLink xlink:role="http://q2ebanking.com/role/CoverPage" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesTable_01a17e83-ae32-7095-a8d9-cc2fe69df9cc" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityAddressesTable_01a17e83-ae32-7095-a8d9-cc2fe69df9cc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressesAddressTypeAxis_01a17e83-ae32-739d-9957-115c788e5f2f" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressesAddressTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_EntityAddressesTable_01a17e83-ae32-7095-a8d9-cc2fe69df9cc" xlink:to="loc_dei_EntityAddressesAddressTypeAxis_01a17e83-ae32-739d-9957-115c788e5f2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AddressTypeDomain_01a17e83-ae32-739d-9957-115c788e5f2f_default" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_AddressTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_EntityAddressesAddressTypeAxis_01a17e83-ae32-739d-9957-115c788e5f2f" xlink:to="loc_dei_AddressTypeDomain_01a17e83-ae32-739d-9957-115c788e5f2f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AddressTypeDomain_01a17e83-ae32-7c75-885a-89c31f8fa186" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_AddressTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_EntityAddressesAddressTypeAxis_01a17e83-ae32-739d-9957-115c788e5f2f" xlink:to="loc_dei_AddressTypeDomain_01a17e83-ae32-7c75-885a-89c31f8fa186" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_FormerAddressMember_01a17e83-ae32-745d-8f7d-b7e1195adacd" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_FormerAddressMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_AddressTypeDomain_01a17e83-ae32-7c75-885a-89c31f8fa186" xlink:to="loc_dei_FormerAddressMember_01a17e83-ae32-745d-8f7d-b7e1195adacd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_01a17e83-ae32-7e75-9387-73cc82d933b8" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_DocumentType_01a17e83-ae32-7e75-9387-73cc82d933b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_01a17e83-ae32-756a-aa4a-61e4fe4b7e87" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_DocumentPeriodEndDate_01a17e83-ae32-756a-aa4a-61e4fe4b7e87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_01a17e83-ae32-79c1-b963-b3717ac11226" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityRegistrantName_01a17e83-ae32-79c1-b963-b3717ac11226" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_01a17e83-ae32-7709-a0c2-6a18e6da8cea" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityIncorporationStateCountryCode_01a17e83-ae32-7709-a0c2-6a18e6da8cea" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_01a17e83-ae32-7b50-8637-67d2002e138b" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityFileNumber_01a17e83-ae32-7b50-8637-67d2002e138b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_01a17e83-ae32-78f6-abec-291acadea05d" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityTaxIdentificationNumber_01a17e83-ae32-78f6-abec-291acadea05d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_01a17e83-ae32-7214-87d1-383b38f0890b" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityAddressAddressLine1_01a17e83-ae32-7214-87d1-383b38f0890b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine2_01a17e83-ae32-72d6-87f8-24920a637088" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressAddressLine2"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityAddressAddressLine2_01a17e83-ae32-72d6-87f8-24920a637088" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_01a17e83-ae32-71bb-bb4b-30e84cc459d1" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityAddressCityOrTown_01a17e83-ae32-71bb-bb4b-30e84cc459d1" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_01a17e83-ae32-74d4-bd88-96f1be130ac3" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityAddressStateOrProvince_01a17e83-ae32-74d4-bd88-96f1be130ac3" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_01a17e83-ae32-7d33-b471-dcd5a04e5b00" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityAddressPostalZipCode_01a17e83-ae32-7d33-b471-dcd5a04e5b00" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_01a17e83-ae32-7183-923f-e17af79e259c" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_CityAreaCode_01a17e83-ae32-7183-923f-e17af79e259c" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_01a17e83-ae32-7a9e-be95-5a523311802b" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_LocalPhoneNumber_01a17e83-ae32-7a9e-be95-5a523311802b" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_01a17e83-ae32-7529-87b9-62470cc515cf" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_Security12bTitle_01a17e83-ae32-7529-87b9-62470cc515cf" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_01a17e83-ae32-7d6d-9c0f-6642fa107f02" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_TradingSymbol_01a17e83-ae32-7d6d-9c0f-6642fa107f02" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_01a17e83-ae32-778d-916f-085339a624a3" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_SecurityExchangeName_01a17e83-ae32-778d-916f-085339a624a3" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_01a17e83-ae32-7b6b-97d8-4789f564270a" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_WrittenCommunications"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_WrittenCommunications_01a17e83-ae32-7b6b-97d8-4789f564270a" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_01a17e83-ae32-743c-a7a4-5f5f0214314f" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_SolicitingMaterial"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_SolicitingMaterial_01a17e83-ae32-743c-a7a4-5f5f0214314f" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_01a17e83-ae32-75aa-8936-1239cb5f3dae" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_PreCommencementTenderOffer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_PreCommencementTenderOffer_01a17e83-ae32-75aa-8936-1239cb5f3dae" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_01a17e83-ae32-7cca-8860-b039065e9e73" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_01a17e83-ae32-7cca-8860-b039065e9e73" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_01a17e83-ae32-7dfd-9402-dd9032cd768a" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityEmergingGrowthCompany_01a17e83-ae32-7dfd-9402-dd9032cd768a" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_01a17e83-ae32-790d-9175-4e1982002b9a" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_EntityCentralIndexKey_01a17e83-ae32-790d-9175-4e1982002b9a" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_01a17e83-ae32-747e-a88a-03ba868819ab" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityAddressesLineItems_01a17e83-ae32-7f92-995b-503b4917afd7" xlink:to="loc_dei_AmendmentFlag_01a17e83-ae32-747e-a88a-03ba868819ab" xlink:type="arc" order="22"/>
  </link:definitionLink>
</link:linkbase>
