EATON VANCE SPECIAL INVESTMENT TRUST

One Post Office Square

Boston, MA 02109

Telephone: (617) 482-8260

CERTIFICATION

Pursuant to Rule 497(j) under the Securities Act of 1933, as amended, and Regulation S-T, Eaton Vance Special Investment Trust (the “Registrant”) (1933 Act File No. 002-27962) certifies (a) that the form of statement of additional information dated October 5, 2026 used with respect to the following series of the Registrant does not differ from the form contained in Post-Effective Amendment No. 214 (“Amendment No. 214”) to the Registrant’s Registration Statement on Form N-1A, and (b) that Amendment No. 214 was filed electronically with the Securities and Exchange Commission (Accession No. 0001133228-26-013109) on October 5, 2026.

Eaton Vance Dividend Builder Fund

Eaton Vance Growth Fund

Eaton Vance Large-Cap Value Fund

Eaton Vance Small-Cap Fund

Date: October 9, 2026