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        <link:definition>995327 - Disclosure - Investment in SAFE</link:definition>
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        <link:definition>996352 - Disclosure - Asset Acquisition (Tables)</link:definition>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/RevenueTables" id="RevenueTables">
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/NonOperatingExpensesTables" id="NonOperatingExpensesTables">
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        <link:usedOn>link:calculationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/EarningsLosspershareTables" id="EarningsLosspershareTables">
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/FutureOperationsandGoingConcernDetails" id="FutureOperationsandGoingConcernDetails">
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/BasisofPresentationScheduleofStatementsofChangesinShareholdersEquityDetails" id="BasisofPresentationScheduleofStatementsofChangesinShareholdersEquityDetails">
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/ConcentrationofCreditRisksScheduleofRevenueandReceivablesConcentrationDetails" id="ConcentrationofCreditRisksScheduleofRevenueandReceivablesConcentrationDetails">
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/BusinessCombinationScheduleofPreliminaryAllocationoftheAcquisitionDateFairValueDetails" id="BusinessCombinationScheduleofPreliminaryAllocationoftheAcquisitionDateFairValueDetails">
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/AccountsReceivableScheduleofAccountsReceivableBalanceDetails" id="AccountsReceivableScheduleofAccountsReceivableBalanceDetails">
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
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        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/PropertyPlantandEquipmentScheduleofPropertyPlantandEquipmentDetails" id="PropertyPlantandEquipmentScheduleofPropertyPlantandEquipmentDetails">
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        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/PropertyPlantandEquipmentScheduleofEstimatedUsefulLivesDetails" id="PropertyPlantandEquipmentScheduleofEstimatedUsefulLivesDetails">
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/LeasesScheduleofRightofUseAssetsandLeaseLiabilitiesDetails" id="LeasesScheduleofRightofUseAssetsandLeaseLiabilitiesDetails">
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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        <link:usedOn>link:presentationLink</link:usedOn>
        <link:usedOn>link:calculationLink</link:usedOn>
        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:definitionLink</link:usedOn>
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        <link:usedOn>link:calculationLink</link:usedOn>
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      <link:roleType roleURI="http://wisekey.com/20260630/role/InvestmentinSAFEDetails" id="InvestmentinSAFEDetails">
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        <link:usedOn>link:calculationLink</link:usedOn>
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