<?xml version="1.0" encoding="us-ascii"?>
<!-- XBRL document created with Praxa -->
<!-- Copyright (c) 2026 Praxa LLC. All Rights Reserved.-->
<!-- Creation Date: 2026-09-25T14:34:37.2245+00:00 -->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/notAll" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#notAll" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/CondensedConsolidatedStatementsofChangesinShareholdersEquityUnaudited" xlink:href="enlv-20260630.xsd#CondensedConsolidatedStatementsofChangesinShareholdersEquityUnaudited" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/SignificantAccountingPoliciesScheduleofComputingEarningsLossPerShareandtheEffectonIncomeLossDetails" xlink:href="enlv-20260630.xsd#SignificantAccountingPoliciesScheduleofComputingEarningsLossPerShareandtheEffectonIncomeLossDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/SignificantAccountingPoliciesDetails" xlink:href="enlv-20260630.xsd#SignificantAccountingPoliciesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetails" xlink:href="enlv-20260630.xsd#ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/EquityDetails" xlink:href="enlv-20260630.xsd#EquityDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/CommitmentsandContingentLiabilitiesDetails" xlink:href="enlv-20260630.xsd#CommitmentsandContingentLiabilitiesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DigitalAssetsScheduleofChangesinCompanysDigitalAssetsDetails" xlink:href="enlv-20260630.xsd#DigitalAssetsScheduleofChangesinCompanysDigitalAssetsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DigitalAssetsScheduleofDigitalAssetsDetails" xlink:href="enlv-20260630.xsd#DigitalAssetsScheduleofDigitalAssetsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DigitalAssetsDetails" xlink:href="enlv-20260630.xsd#DigitalAssetsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ShareBasedCompensationScheduleofExercisePricesandRemainingContractualLifeDetails" xlink:href="enlv-20260630.xsd#ShareBasedCompensationScheduleofExercisePricesandRemainingContractualLifeDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DigitalAssetsPurchaseOptionScheduleofValuationAssumptionDetails" xlink:href="enlv-20260630.xsd#DigitalAssetsPurchaseOptionScheduleofValuationAssumptionDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetails" xlink:href="enlv-20260630.xsd#DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/EquityScheduleofWarrantsOutstandingDetails" xlink:href="enlv-20260630.xsd#EquityScheduleofWarrantsOutstandingDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ShareBasedCompensationDetails" xlink:href="enlv-20260630.xsd#ShareBasedCompensationDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ShortTermDepositsScheduleofShortTermDepositsDetails" xlink:href="enlv-20260630.xsd#ShortTermDepositsScheduleofShortTermDepositsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetails" xlink:href="enlv-20260630.xsd#ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/PropertyandEquipmentScheduleofPropertyandEquipmentNetDetails" xlink:href="enlv-20260630.xsd#PropertyandEquipmentScheduleofPropertyandEquipmentNetDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/EventsSubsequenttotheBalanceSheetDateDetails" xlink:href="enlv-20260630.xsd#EventsSubsequenttotheBalanceSheetDateDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetails" xlink:href="enlv-20260630.xsd#FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/EquityScheduleofWarrantsActivityDetails" xlink:href="enlv-20260630.xsd#EquityScheduleofWarrantsActivityDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ShareBasedCompensationScheduleofOptionsGrantedDetails" xlink:href="enlv-20260630.xsd#ShareBasedCompensationScheduleofOptionsGrantedDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DerivativeLiabilityScheduleofDerivativeLiabilitiesMeasuredUsingLevel3InputsDetails" xlink:href="enlv-20260630.xsd#DerivativeLiabilityScheduleofDerivativeLiabilitiesMeasuredUsingLevel3InputsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/DerivativeLiabilityScheduleofValuationAssumptionofDerivativeLiabilityDetails" xlink:href="enlv-20260630.xsd#DerivativeLiabilityScheduleofValuationAssumptionofDerivativeLiabilityDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://enlivex.com/20260630/role/ConvertibleDebtDetails" xlink:href="enlv-20260630.xsd#ConvertibleDebtDetails" xlink:type="simple" />
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/CondensedConsolidatedStatementsofChangesinShareholdersEquityUnaudited" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalMember" xlink:label="us-gaap_AdditionalPaidInCapitalMember_1066" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsMember" xlink:label="us-gaap_RetainedEarningsMember_1068" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_1065" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember" xlink:label="us-gaap_AccumulatedOtherComprehensiveIncomeMember_1067" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity" xlink:label="us-gaap_StockholdersEquity_1071" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_1064" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems" xlink:label="us-gaap_StatementLineItems_1070" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable" xlink:label="us-gaap_StatementTable_1063" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss" xlink:label="us-gaap_NetIncomeLoss_1080" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding" xlink:label="us-gaap_CommonStockSharesOutstanding_1082" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodValueNewIssues_1073" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardGross" xlink:label="us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardGross_1077" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities" xlink:label="us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_2183" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities" xlink:label="us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_2184" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_AdjustmentsToAdditionalPaidInCapitalSettlementOfDerivativeLiability" xlink:label="enlv_AdjustmentsToAdditionalPaidInCapitalSettlementOfDerivativeLiability_2204" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1069" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_1079" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1074" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross" xlink:label="us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_1078" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1069_default" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1069" xlink:to="us-gaap_AdditionalPaidInCapitalMember_1066" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1069" xlink:to="us-gaap_RetainedEarningsMember_1068" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1069" xlink:to="us-gaap_CommonStockMember_1065" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1069" xlink:to="us-gaap_AccumulatedOtherComprehensiveIncomeMember_1067" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockholdersEquity_1071" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_1063" xlink:to="us-gaap_StatementEquityComponentsAxis_1064" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StatementTable_1063" />
    <link:definitionArc xlink:type="arc" order="21.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_NetIncomeLoss_1080" />
    <link:definitionArc xlink:type="arc" order="25.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_CommonStockSharesOutstanding_1082" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockIssuedDuringPeriodValueNewIssues_1073" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardGross_1077" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_2183" />
    <link:definitionArc xlink:type="arc" order="18.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_2184" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="enlv_AdjustmentsToAdditionalPaidInCapitalSettlementOfDerivativeLiability_2204" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_1064" xlink:to="us-gaap_EquityComponentDomain_1069" />
    <link:definitionArc xlink:type="arc" order="20.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_1079" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1074" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1070" xlink:to="us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_1078" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_1064" xlink:to="us-gaap_EquityComponentDomain_1069_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/SignificantAccountingPoliciesScheduleofComputingEarningsLossPerShareandtheEffectonIncomeLossDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConvertibleDebtSecuritiesMember" xlink:label="us-gaap_ConvertibleDebtSecuritiesMember_2248" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember" xlink:label="us-gaap_EmployeeStockOptionMember_2249" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantMember" xlink:label="us-gaap_WarrantMember_2250" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember" xlink:label="us-gaap_RestrictedStockUnitsRSUMember_2251" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WeightedAverageNumberOfConvertibleDebt" xlink:label="enlv_WeightedAverageNumberOfConvertibleDebt_2227" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromContinuingOperations" xlink:label="us-gaap_IncomeLossFromContinuingOperations_1309" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DilutiveSecuritiesAbstract" xlink:label="us-gaap_DilutiveSecuritiesAbstract_1317" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WeightedAverageNumberOfStockOptions" xlink:label="enlv_WeightedAverageNumberOfStockOptions_1319" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WeightedAverageNumberOfWarrants" xlink:label="enlv_WeightedAverageNumberOfWarrants_1321" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareBasicAbstract" xlink:label="us-gaap_EarningsPerShareBasicAbstract_1308" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareBasic" xlink:label="us-gaap_EarningsPerShareBasic_1316" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WeightedAverageNumberOfRestrictedStockUnits" xlink:label="enlv_WeightedAverageNumberOfRestrictedStockUnits_1320" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_AntiDilutiveSecuritiesAbstract" xlink:label="enlv_AntiDilutiveSecuritiesAbstract_2228" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WeightedAverageNumberOfOrdinarySharesIssuableUnderConvertibleDebt" xlink:label="enlv_WeightedAverageNumberOfOrdinarySharesIssuableUnderConvertibleDebt_2229" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount" xlink:label="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_2233" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable" xlink:label="us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_2243" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis" xlink:label="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_2244" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain" xlink:label="us-gaap_AntidilutiveSecuritiesNameDomain_2245" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareBasicLineItems" xlink:label="us-gaap_EarningsPerShareBasicLineItems_2260" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareDiluted" xlink:label="us-gaap_EarningsPerShareDiluted_1323" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding" xlink:label="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_2206" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareDilutedAbstract" xlink:label="us-gaap_EarningsPerShareDilutedAbstract_2207" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic" xlink:label="us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_2205" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain" xlink:label="us-gaap_AntidilutiveSecuritiesNameDomain_2245_default" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AntidilutiveSecuritiesNameDomain_2245" xlink:to="us-gaap_ConvertibleDebtSecuritiesMember_2248" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AntidilutiveSecuritiesNameDomain_2245" xlink:to="us-gaap_EmployeeStockOptionMember_2249" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AntidilutiveSecuritiesNameDomain_2245" xlink:to="us-gaap_WarrantMember_2250" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AntidilutiveSecuritiesNameDomain_2245" xlink:to="us-gaap_RestrictedStockUnitsRSUMember_2251" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DilutiveSecuritiesAbstract_1317" xlink:to="enlv_WeightedAverageNumberOfConvertibleDebt_2227" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareBasicAbstract_1308" xlink:to="us-gaap_IncomeLossFromContinuingOperations_1309" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareDilutedAbstract_2207" xlink:to="us-gaap_DilutiveSecuritiesAbstract_1317" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DilutiveSecuritiesAbstract_1317" xlink:to="enlv_WeightedAverageNumberOfStockOptions_1319" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DilutiveSecuritiesAbstract_1317" xlink:to="enlv_WeightedAverageNumberOfWarrants_1321" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareBasicLineItems_2260" xlink:to="us-gaap_EarningsPerShareBasicAbstract_1308" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareBasicAbstract_1308" xlink:to="us-gaap_EarningsPerShareBasic_1316" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DilutiveSecuritiesAbstract_1317" xlink:to="enlv_WeightedAverageNumberOfRestrictedStockUnits_1320" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareBasicLineItems_2260" xlink:to="enlv_AntiDilutiveSecuritiesAbstract_2228" />
    <link:definitionArc xlink:type="arc" order="145.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_AntiDilutiveSecuritiesAbstract_2228" xlink:to="enlv_WeightedAverageNumberOfOrdinarySharesIssuableUnderConvertibleDebt_2229" />
    <link:definitionArc xlink:type="arc" order="146.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_AntiDilutiveSecuritiesAbstract_2228" xlink:to="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_2233" />
    <link:definitionArc xlink:type="arc" order="155.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_EarningsPerShareBasicLineItems_2260" xlink:to="us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_2243" />
    <link:definitionArc xlink:type="arc" order="156.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_2243" xlink:to="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_2244" />
    <link:definitionArc xlink:type="arc" order="157.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_2244" xlink:to="us-gaap_AntidilutiveSecuritiesNameDomain_2245" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareDilutedAbstract_2207" xlink:to="us-gaap_EarningsPerShareDiluted_1323" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DilutiveSecuritiesAbstract_1317" xlink:to="us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_2206" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareBasicLineItems_2260" xlink:to="us-gaap_EarningsPerShareDilutedAbstract_2207" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EarningsPerShareBasicAbstract_1308" xlink:to="us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_2205" />
    <link:definitionArc xlink:type="arc" order="157.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_2244" xlink:to="us-gaap_AntidilutiveSecuritiesNameDomain_2245_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/SignificantAccountingPoliciesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DecreaseInForeignCurrencyExchangeRateTranslation" xlink:label="enlv_DecreaseInForeignCurrencyExchangeRateTranslation_2349" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ForeignCurrencyExchangeRateTranslation1" xlink:label="us-gaap_ForeignCurrencyExchangeRateTranslation1_2269" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/country/2026/country-2026.xsd#country_US" xlink:label="country_US_2270" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SignificantAccountingPoliciesDetailsTable" xlink:label="enlv_SignificantAccountingPoliciesDetailsTable_1722" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_SegmentGeographicalDomain" xlink:label="srt_SegmentGeographicalDomain_1724" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_StatementGeographicalAxis" xlink:label="srt_StatementGeographicalAxis_1723" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/country/2026/country-2026.xsd#country_IL" xlink:label="country_IL_1725" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ChangeInAccountingEstimateLineItems" xlink:label="us-gaap_ChangeInAccountingEstimateLineItems_1729" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_SegmentGeographicalDomain" xlink:label="srt_SegmentGeographicalDomain_1724_default" />
    <link:definitionArc xlink:type="arc" order="223.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ChangeInAccountingEstimateLineItems_1729" xlink:to="enlv_DecreaseInForeignCurrencyExchangeRateTranslation_2349" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ChangeInAccountingEstimateLineItems_1729" xlink:to="us-gaap_ForeignCurrencyExchangeRateTranslation1_2269" />
    <link:definitionArc xlink:type="arc" order="178.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_SegmentGeographicalDomain_1724" xlink:to="country_US_2270" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ChangeInAccountingEstimateLineItems_1729" xlink:to="enlv_SignificantAccountingPoliciesDetailsTable_1722" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_StatementGeographicalAxis_1723" xlink:to="srt_SegmentGeographicalDomain_1724" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_SignificantAccountingPoliciesDetailsTable_1722" xlink:to="srt_StatementGeographicalAxis_1723" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_SegmentGeographicalDomain_1724" xlink:to="country_IL_1725" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_StatementGeographicalAxis_1723" xlink:to="srt_SegmentGeographicalDomain_1724_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ResearchDevelopmentMember" xlink:label="enlv_ResearchDevelopmentMember_1597" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_GeneralAdministrativeMember" xlink:label="enlv_GeneralAdministrativeMember_1598" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1599" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetailsTable" xlink:label="enlv_ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetailsTable_1594" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_StatementOfIncomeLocationBalancesAxis" xlink:label="enlv_StatementOfIncomeLocationBalancesAxis_2338" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_StatementOfIncomeLocationBalancesAxisDomain" xlink:label="enlv_StatementOfIncomeLocationBalancesAxisDomain_2339" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense" xlink:label="us-gaap_AllocatedShareBasedCompensationExpense_1600" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_StatementOfIncomeLocationBalancesAxisDomain" xlink:label="enlv_StatementOfIncomeLocationBalancesAxisDomain_2339_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_StatementOfIncomeLocationBalancesAxisDomain_2339" xlink:to="enlv_ResearchDevelopmentMember_1597" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_StatementOfIncomeLocationBalancesAxisDomain_2339" xlink:to="enlv_GeneralAdministrativeMember_1598" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1599" xlink:to="enlv_ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetailsTable_1594" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ShareBasedCompensationScheduleofShareBasedCompensationExpenseDetailsTable_1594" xlink:to="enlv_StatementOfIncomeLocationBalancesAxis_2338" />
    <link:definitionArc xlink:type="arc" order="213.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="enlv_StatementOfIncomeLocationBalancesAxis_2338" xlink:to="enlv_StatementOfIncomeLocationBalancesAxisDomain_2339" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1599" xlink:to="us-gaap_AllocatedShareBasedCompensationExpense_1600" />
    <link:definitionArc xlink:type="arc" order="213.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="enlv_StatementOfIncomeLocationBalancesAxis_2338" xlink:to="enlv_StatementOfIncomeLocationBalancesAxisDomain_2339_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/EquityDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ATMAgreementMember" xlink:label="enlv_ATMAgreementMember_1795" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_2321" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_2322" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_2323" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SalesAgreementMember" xlink:label="enlv_SalesAgreementMember_1791" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_HolderMember" xlink:label="enlv_HolderMember_2239" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_2257" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_EquityDetailsTable" xlink:label="enlv_EquityDetailsTable_1788" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_AgreementDomainDomain" xlink:label="enlv_AgreementDomainDomain_1790" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquityReverseStockSplit" xlink:label="us-gaap_StockholdersEquityReverseStockSplit_2319" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_2320" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockLineItems" xlink:label="us-gaap_ClassOfStockLineItems_1803" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock" xlink:label="us-gaap_ProceedsFromIssuanceOfCommonStock_1811" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_IssuanceCosts" xlink:label="enlv_IssuanceCosts_1813" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued" xlink:label="us-gaap_CommonStockSharesIssued_1804" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockParOrStatedValuePerShare" xlink:label="us-gaap_CommonStockParOrStatedValuePerShare_1806" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CommissionPercentage" xlink:label="enlv_CommissionPercentage_1812" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceInitialPublicOffering" xlink:label="us-gaap_ProceedsFromIssuanceInitialPublicOffering_1818" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockConsiderationReceivedOnTransaction" xlink:label="us-gaap_SaleOfStockConsiderationReceivedOnTransaction_1820" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_AgreementAxis" xlink:label="enlv_AgreementAxis_1789" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2237" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_2238" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesAuthorized" xlink:label="us-gaap_CommonStockSharesAuthorized_2252" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_2255" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_2256" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsOfStockIssuanceCosts" xlink:label="us-gaap_PaymentsOfStockIssuanceCosts_2383" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_2321_default" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_AgreementDomainDomain" xlink:label="enlv_AgreementDomainDomain_1790_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_2238_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_2256_default" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_AgreementDomainDomain_1790" xlink:to="enlv_ATMAgreementMember_1795" />
    <link:definitionArc xlink:type="arc" order="202.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_2320" xlink:to="srt_RangeMember_2321" />
    <link:definitionArc xlink:type="arc" order="203.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_2321" xlink:to="srt_MaximumMember_2322" />
    <link:definitionArc xlink:type="arc" order="204.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_2321" xlink:to="srt_MinimumMember_2323" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_AgreementDomainDomain_1790" xlink:to="enlv_SalesAgreementMember_1791" />
    <link:definitionArc xlink:type="arc" order="152.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_2238" xlink:to="enlv_HolderMember_2239" />
    <link:definitionArc xlink:type="arc" order="167.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_2256" xlink:to="us-gaap_CommonStockMember_2257" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="enlv_EquityDetailsTable_1788" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="enlv_AgreementAxis_1789" xlink:to="enlv_AgreementDomainDomain_1790" />
    <link:definitionArc xlink:type="arc" order="200.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_StockholdersEquityReverseStockSplit_2319" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EquityDetailsTable_1788" xlink:to="srt_RangeAxis_2320" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_ProceedsFromIssuanceOfCommonStock_1811" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="enlv_IssuanceCosts_1813" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_CommonStockSharesIssued_1804" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_CommonStockParOrStatedValuePerShare_1806" />
    <link:definitionArc xlink:type="arc" order="18.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="enlv_CommissionPercentage_1812" />
    <link:definitionArc xlink:type="arc" order="24.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_ProceedsFromIssuanceInitialPublicOffering_1818" />
    <link:definitionArc xlink:type="arc" order="26.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_SaleOfStockConsiderationReceivedOnTransaction_1820" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EquityDetailsTable_1788" xlink:to="enlv_AgreementAxis_1789" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EquityDetailsTable_1788" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2237" />
    <link:definitionArc xlink:type="arc" order="151.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2237" xlink:to="us-gaap_RelatedPartyDomain_2238" />
    <link:definitionArc xlink:type="arc" order="163.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_CommonStockSharesAuthorized_2252" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EquityDetailsTable_1788" xlink:to="us-gaap_StatementEquityComponentsAxis_2255" />
    <link:definitionArc xlink:type="arc" order="166.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_2255" xlink:to="us-gaap_EquityComponentDomain_2256" />
    <link:definitionArc xlink:type="arc" order="247.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockLineItems_1803" xlink:to="us-gaap_PaymentsOfStockIssuanceCosts_2383" />
    <link:definitionArc xlink:type="arc" order="202.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_2320" xlink:to="srt_RangeMember_2321_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="enlv_AgreementAxis_1789" xlink:to="enlv_AgreementDomainDomain_1790_default" />
    <link:definitionArc xlink:type="arc" order="151.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_2237" xlink:to="us-gaap_RelatedPartyDomain_2238_default" />
    <link:definitionArc xlink:type="arc" order="166.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_2255" xlink:to="us-gaap_EquityComponentDomain_2256_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/CommitmentsandContingentLiabilitiesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_1775" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_IsraeliInnovationAuthorityMember" xlink:label="enlv_IsraeliInnovationAuthorityMember_1772" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1774" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_1776" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CommitmentsandContingentLiabilitiesDetailsTable" xlink:label="enlv_CommitmentsandContingentLiabilitiesDetailsTable_1769" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionDomain" xlink:label="us-gaap_RelatedPartyTransactionDomain_1771" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_1773" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherCommitmentsLineItems" xlink:label="us-gaap_OtherCommitmentsLineItems_1777" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_PercentageOfGrantsRepayments" xlink:label="enlv_PercentageOfGrantsRepayments_1779" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionAxis" xlink:label="us-gaap_RelatedPartyTransactionAxis_1770" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RoyaltiesInSalesPercentage" xlink:label="enlv_RoyaltiesInSalesPercentage_1778" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GrantsReceivable" xlink:label="us-gaap_GrantsReceivable_1780" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1774_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionDomain" xlink:label="us-gaap_RelatedPartyTransactionDomain_1771_default" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1774" xlink:to="srt_MinimumMember_1775" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyTransactionDomain_1771" xlink:to="enlv_IsraeliInnovationAuthorityMember_1772" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_1773" xlink:to="srt_RangeMember_1774" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1774" xlink:to="srt_MaximumMember_1776" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_OtherCommitmentsLineItems_1777" xlink:to="enlv_CommitmentsandContingentLiabilitiesDetailsTable_1769" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionAxis_1770" xlink:to="us-gaap_RelatedPartyTransactionDomain_1771" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_CommitmentsandContingentLiabilitiesDetailsTable_1769" xlink:to="srt_RangeAxis_1773" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OtherCommitmentsLineItems_1777" xlink:to="enlv_PercentageOfGrantsRepayments_1779" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_CommitmentsandContingentLiabilitiesDetailsTable_1769" xlink:to="us-gaap_RelatedPartyTransactionAxis_1770" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OtherCommitmentsLineItems_1777" xlink:to="enlv_RoyaltiesInSalesPercentage_1778" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OtherCommitmentsLineItems_1777" xlink:to="us-gaap_GrantsReceivable_1780" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_1773" xlink:to="srt_RangeMember_1774_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionAxis_1770" xlink:to="us-gaap_RelatedPartyTransactionDomain_1771_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CurrencyAxis" xlink:label="srt_CurrencyAxis_1358" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_AllCurrenciesDomain" xlink:label="currency_AllCurrenciesDomain_2168" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetailsTable" xlink:label="enlv_ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetailsTable_1357" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_USD" xlink:label="currency_USD_1359" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfInvestmentsLineItems" xlink:label="us-gaap_ScheduleOfInvestmentsLineItems_1361" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_ILS" xlink:label="currency_ILS_1360" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_InterestBearingDepositsInBanksAverageYield" xlink:label="srt_InterestBearingDepositsInBanksAverageYield_1362" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_AllCurrenciesDomain" xlink:label="currency_AllCurrenciesDomain_2168_default" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetailsTable_1357" xlink:to="srt_CurrencyAxis_1358" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_CurrencyAxis_1358" xlink:to="currency_AllCurrenciesDomain_2168" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ScheduleOfInvestmentsLineItems_1361" xlink:to="enlv_ShortTermDepositsScheduleofShortTermDepositsParentheticalsDetailsTable_1357" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="currency_AllCurrenciesDomain_2168" xlink:to="currency_USD_1359" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="currency_AllCurrenciesDomain_2168" xlink:to="currency_ILS_1360" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfInvestmentsLineItems_1361" xlink:to="srt_InterestBearingDepositsInBanksAverageYield_1362" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_CurrencyAxis_1358" xlink:to="currency_AllCurrenciesDomain_2168_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DigitalAssetsScheduleofChangesinCompanysDigitalAssetsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_USDTDigitalAssetMember" xlink:label="enlv_USDTDigitalAssetMember_2294" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsMember" xlink:label="enlv_RAINDigitalAssetsMember_2295" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetDomain" xlink:label="srt_CryptoAssetDomain_2291" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems" xlink:label="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2292" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_PurchaseOfDigitalAsset" xlink:label="enlv_PurchaseOfDigitalAsset_1375" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable" xlink:label="enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable_2289" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetAxis" xlink:label="srt_CryptoAssetAxis_2290" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GainLossOnInvestments" xlink:label="us-gaap_GainLossOnInvestments_1379" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetFairValue" xlink:label="us-gaap_CryptoAssetFairValue_1380" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SalesOfDigitalAssets" xlink:label="enlv_SalesOfDigitalAssets_1374" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetDomain" xlink:label="srt_CryptoAssetDomain_2291_default" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_CryptoAssetDomain_2291" xlink:to="enlv_USDTDigitalAssetMember_2294" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_CryptoAssetDomain_2291" xlink:to="enlv_RAINDigitalAssetsMember_2295" />
    <link:definitionArc xlink:type="arc" order="185.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_CryptoAssetAxis_2290" xlink:to="srt_CryptoAssetDomain_2291" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2292" xlink:to="enlv_PurchaseOfDigitalAsset_1375" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2292" xlink:to="enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable_2289" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable_2289" xlink:to="srt_CryptoAssetAxis_2290" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2292" xlink:to="us-gaap_GainLossOnInvestments_1379" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2292" xlink:to="us-gaap_CryptoAssetFairValue_1380" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2292" xlink:to="enlv_SalesOfDigitalAssets_1374" />
    <link:definitionArc xlink:type="arc" order="185.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_CryptoAssetAxis_2290" xlink:to="srt_CryptoAssetDomain_2291_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DigitalAssetsScheduleofDigitalAssetsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsMember" xlink:label="enlv_RAINDigitalAssetsMember_2316" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_USDTDigitalAssetMember" xlink:label="enlv_USDTDigitalAssetMember_2317" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsCurrentTokens" xlink:label="enlv_RAINDigitalAssetsCurrentTokens_2296" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsNoncurrentTokens" xlink:label="enlv_RAINDigitalAssetsNoncurrentTokens_2297" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsTokens" xlink:label="enlv_RAINDigitalAssetsTokens_2298" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CryptoAssetTotalUSDTCurrent" xlink:label="enlv_CryptoAssetTotalUSDTCurrent_2303" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable" xlink:label="enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable_2313" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetAxis" xlink:label="srt_CryptoAssetAxis_2314" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetDomain" xlink:label="srt_CryptoAssetDomain_2315" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems" xlink:label="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CryptoAssetsTotalUSDTCurrentTokens" xlink:label="enlv_CryptoAssetsTotalUSDTCurrentTokens_2367" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetFairValueCurrent" xlink:label="us-gaap_CryptoAssetFairValueCurrent_1383" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetFairValueNoncurrent" xlink:label="us-gaap_CryptoAssetFairValueNoncurrent_1384" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetFairValue" xlink:label="us-gaap_CryptoAssetFairValue_1385" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetDomain" xlink:label="srt_CryptoAssetDomain_2315_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_CryptoAssetDomain_2315" xlink:to="enlv_RAINDigitalAssetsMember_2316" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_CryptoAssetDomain_2315" xlink:to="enlv_USDTDigitalAssetMember_2317" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="enlv_RAINDigitalAssetsCurrentTokens_2296" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="enlv_RAINDigitalAssetsNoncurrentTokens_2297" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="enlv_RAINDigitalAssetsTokens_2298" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="enlv_CryptoAssetTotalUSDTCurrent_2303" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable_2313" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_DigitalAssetsScheduleofComponentsofNetGainsRecognizedinEarningstoRAINDigitalAssetsDetailsTable_2313" xlink:to="srt_CryptoAssetAxis_2314" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_CryptoAssetAxis_2314" xlink:to="srt_CryptoAssetDomain_2315" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="enlv_CryptoAssetsTotalUSDTCurrentTokens_2367" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="us-gaap_CryptoAssetFairValueCurrent_1383" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="us-gaap_CryptoAssetFairValueNoncurrent_1384" />
    <link:definitionArc xlink:type="arc" order="18.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_2318" xlink:to="us-gaap_CryptoAssetFairValue_1385" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_CryptoAssetAxis_2314" xlink:to="srt_CryptoAssetDomain_2315_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DigitalAssetsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsMember" xlink:label="enlv_RAINDigitalAssetsMember_2335" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_USDTDigitalAssetMember" xlink:label="enlv_USDTDigitalAssetMember_2336" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINDigitalAssetsTokens" xlink:label="enlv_RAINDigitalAssetsTokens_2328" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetAxis" xlink:label="srt_CryptoAssetAxis_2331" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetDomain" xlink:label="srt_CryptoAssetDomain_2332" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DigitalAssetsDetailsTable" xlink:label="enlv_DigitalAssetsDetailsTable_1741" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems" xlink:label="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_1745" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CryptoAssetsFairValue" xlink:label="enlv_CryptoAssetsFairValue_2369" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CryptoAssetDomain" xlink:label="srt_CryptoAssetDomain_2332_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_CryptoAssetDomain_2332" xlink:to="enlv_RAINDigitalAssetsMember_2335" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_CryptoAssetDomain_2332" xlink:to="enlv_USDTDigitalAssetMember_2336" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_1745" xlink:to="enlv_RAINDigitalAssetsTokens_2328" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_DigitalAssetsDetailsTable_1741" xlink:to="srt_CryptoAssetAxis_2331" />
    <link:definitionArc xlink:type="arc" order="208.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_CryptoAssetAxis_2331" xlink:to="srt_CryptoAssetDomain_2332" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_1745" xlink:to="enlv_DigitalAssetsDetailsTable_1741" />
    <link:definitionArc xlink:type="arc" order="237.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_CryptoAssetRealizedGainAndLossFromDispositionLineItems_1745" xlink:to="enlv_CryptoAssetsFairValue_2369" />
    <link:definitionArc xlink:type="arc" order="208.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_CryptoAssetAxis_2331" xlink:to="srt_CryptoAssetDomain_2332_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ShareBasedCompensationScheduleofExercisePricesandRemainingContractualLifeDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceFiveMember" xlink:label="enlv_ExercisePriceFiveMember_1556" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceSevenMember" xlink:label="enlv_ExercisePriceSevenMember_1558" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceNineMember" xlink:label="enlv_ExercisePriceNineMember_1560" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceElavenMember" xlink:label="enlv_ExercisePriceElavenMember_1562" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceThirteenMember" xlink:label="enlv_ExercisePriceThirteenMember_1564" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceFifteenMember" xlink:label="enlv_ExercisePriceFifteenMember_1566" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockOptionMember" xlink:label="us-gaap_StockOptionMember_1549" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceTwoMember" xlink:label="enlv_ExercisePriceTwoMember_1553" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceFourMember" xlink:label="enlv_ExercisePriceFourMember_1555" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceSixMember" xlink:label="enlv_ExercisePriceSixMember_1557" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisesPriceEightMember" xlink:label="enlv_ExercisesPriceEightMember_1559" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceTenMember" xlink:label="enlv_ExercisePriceTenMember_1561" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceTwelveMember" xlink:label="enlv_ExercisePriceTwelveMember_1563" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceFourteenMember" xlink:label="enlv_ExercisePriceFourteenMember_1565" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceSixteenMember" xlink:label="enlv_ExercisePriceSixteenMember_1567" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceOneMember" xlink:label="enlv_ExercisePriceOneMember_1552" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceThreeMember" xlink:label="enlv_ExercisePriceThreeMember_1554" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfOutstandingOptions" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfOutstandingOptions_1572" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingAggregateIntrinsicValue" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingAggregateIntrinsicValue_1574" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeInstrumentRiskAxis" xlink:label="us-gaap_DerivativeInstrumentRiskAxis_1547" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_1573" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLowerRangeLimit" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLowerRangeLimit_1571" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions_1575" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_1548" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1550" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable" xlink:label="us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_1546" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_1548_default" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceFiveMember_1556" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceSevenMember_1558" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceNineMember_1560" />
    <link:definitionArc xlink:type="arc" order="21.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceElavenMember_1562" />
    <link:definitionArc xlink:type="arc" order="23.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceThirteenMember_1564" />
    <link:definitionArc xlink:type="arc" order="25.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceFifteenMember_1566" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain_1548" xlink:to="us-gaap_StockOptionMember_1549" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceTwoMember_1553" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceFourMember_1555" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceSixMember_1557" />
    <link:definitionArc xlink:type="arc" order="18.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisesPriceEightMember_1559" />
    <link:definitionArc xlink:type="arc" order="20.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceTenMember_1561" />
    <link:definitionArc xlink:type="arc" order="22.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceTwelveMember_1563" />
    <link:definitionArc xlink:type="arc" order="24.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceFourteenMember_1565" />
    <link:definitionArc xlink:type="arc" order="26.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceSixteenMember_1567" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceOneMember_1552" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" xlink:to="enlv_ExercisePriceThreeMember_1554" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfOutstandingOptions_1572" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingAggregateIntrinsicValue_1574" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_1546" xlink:to="us-gaap_DerivativeInstrumentRiskAxis_1547" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1550" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_1573" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLowerRangeLimit_1571" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeNumberOfExercisableOptions_1575" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_1547" xlink:to="us-gaap_DerivativeContractTypeDomain_1548" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_1546" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1550" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeLineItems_1570" xlink:to="us-gaap_ScheduleOfShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeTable_1546" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1550" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_1551_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_1547" xlink:to="us-gaap_DerivativeContractTypeDomain_1548_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DigitalAssetsPurchaseOptionScheduleofValuationAssumptionDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockOptionMember" xlink:label="us-gaap_StockOptionMember_1405" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_2170" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_1403" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_1407" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CryptoAssetMarketPrice" xlink:label="enlv_CryptoAssetMarketPrice_1409" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeInstrumentRiskAxis" xlink:label="us-gaap_DerivativeInstrumentRiskAxis_1404" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_1408" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_1410" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice_2201" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_2170_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain_2170" xlink:to="us-gaap_StockOptionMember_1405" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_1404" xlink:to="us-gaap_DerivativeContractTypeDomain_2170" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" xlink:to="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_1403" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_1407" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" xlink:to="enlv_CryptoAssetMarketPrice_1409" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_1403" xlink:to="us-gaap_DerivativeInstrumentRiskAxis_1404" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_1408" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_1410" />
    <link:definitionArc xlink:type="arc" order="130.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice_2201" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_1404" xlink:to="us-gaap_DerivativeContractTypeDomain_2170_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_RAINOptionMember" xlink:label="enlv_RAINOptionMember_1415" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member" xlink:label="us-gaap_FairValueInputsLevel2Member_1417" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2172" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetailsTable" xlink:label="enlv_DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetailsTable_1413" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DigitalAssetsPurchase" xlink:label="enlv_DigitalAssetsPurchase_1419" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CryptoAssetChangeInFairValueRecognizedInEarnings" xlink:label="enlv_CryptoAssetChangeInFairValueRecognizedInEarnings_1421" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeInstrumentRiskAxis" xlink:label="us-gaap_DerivativeInstrumentRiskAxis_1414" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:label="us-gaap_FairValueByFairValueHierarchyLevelAxis_1416" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems" xlink:label="us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_1418" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_FairValueUponInitialRecognition" xlink:label="enlv_FairValueUponInitialRecognition_1420" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_2171" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_CryptoAssetSettlementsOrConversions" xlink:label="enlv_CryptoAssetSettlementsOrConversions_2203" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2172_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_2171_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain_2171" xlink:to="enlv_RAINOptionMember_1415" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2172" xlink:to="us-gaap_FairValueInputsLevel2Member_1417" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis_1416" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2172" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_1418" xlink:to="enlv_DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetailsTable_1413" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_1418" xlink:to="enlv_DigitalAssetsPurchase_1419" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_1418" xlink:to="enlv_CryptoAssetChangeInFairValueRecognizedInEarnings_1421" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetailsTable_1413" xlink:to="us-gaap_DerivativeInstrumentRiskAxis_1414" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_DigitalAssetsPurchaseOptionScheduleofReconciliationOfOptionMeasuredUsingLevel2InputsDetailsTable_1413" xlink:to="us-gaap_FairValueByFairValueHierarchyLevelAxis_1416" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_1418" xlink:to="enlv_FairValueUponInitialRecognition_1420" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_1414" xlink:to="us-gaap_DerivativeContractTypeDomain_2171" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisUnobservableInputReconciliationLineItems_1418" xlink:to="enlv_CryptoAssetSettlementsOrConversions_2203" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis_1416" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2172_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_1414" xlink:to="us-gaap_DerivativeContractTypeDomain_2171_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/EquityScheduleofWarrantsOutstandingDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceFourMember" xlink:label="enlv_ExercisePriceFourMember_1496" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceSixMember" xlink:label="enlv_ExercisePriceSixMember_1498" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePricesEightMember" xlink:label="enlv_ExercisePricesEightMember_1500" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePricesOneMember" xlink:label="enlv_ExercisePricesOneMember_1493" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceThreeMember" xlink:label="enlv_ExercisePriceThreeMember_1495" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePriceFiveMember" xlink:label="enlv_ExercisePriceFiveMember_1497" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePricesSevenMember" xlink:label="enlv_ExercisePricesSevenMember_1499" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ExercisePricesTwoMember" xlink:label="enlv_ExercisePricesTwoMember_1494" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_EquityScheduleofWarrantsOutstandingDetailsTable" xlink:label="enlv_EquityScheduleofWarrantsOutstandingDetailsTable_1491" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightOutstanding" xlink:label="us-gaap_ClassOfWarrantOrRightOutstanding_1504" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WarrantsAndRightsOutstandingIssuanceDate" xlink:label="enlv_WarrantsAndRightsOutstandingIssuanceDate_1506" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightLineItems" xlink:label="us-gaap_ClassOfWarrantOrRightLineItems_1503" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1" xlink:label="us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_1505" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_WarrantsAndRightsOutstandingExpirationDate" xlink:label="enlv_WarrantsAndRightsOutstandingExpirationDate_1507" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1492" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain" xlink:label="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174_default" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePriceFourMember_1496" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePriceSixMember_1498" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePricesEightMember_1500" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePricesOneMember_1493" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePriceThreeMember_1495" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePriceFiveMember_1497" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePricesSevenMember_1499" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" xlink:to="enlv_ExercisePricesTwoMember_1494" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1492" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1503" xlink:to="enlv_EquityScheduleofWarrantsOutstandingDetailsTable_1491" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1503" xlink:to="us-gaap_ClassOfWarrantOrRightOutstanding_1504" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1503" xlink:to="enlv_WarrantsAndRightsOutstandingIssuanceDate_1506" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1503" xlink:to="us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_1505" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1503" xlink:to="enlv_WarrantsAndRightsOutstandingExpirationDate_1507" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EquityScheduleofWarrantsOutstandingDetailsTable_1491" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1492" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansByExercisePriceRangeAxis_1492" xlink:to="us-gaap_ShareBasedCompensationSharesAuthorizedUnderStockOptionPlansExercisePriceRangeDomain_2174_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/EquityScheduleofWarrantsActivityDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantMember" xlink:label="us-gaap_WarrantMember_1477" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsForfeituresAndExpirations" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsForfeituresAndExpirations_1485" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_1487" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightAxis" xlink:label="us-gaap_ClassOfWarrantOrRightAxis_1476" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightLineItems" xlink:label="us-gaap_ClassOfWarrantOrRightLineItems_1478" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_1480" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightTable" xlink:label="us-gaap_ClassOfWarrantOrRightTable_1475" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsIssued" xlink:label="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsIssued_1481" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised_1483" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsExercisedInPeriodWeightedAverageGrantDateFairValue" xlink:label="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsExercisedInPeriodWeightedAverageGrantDateFairValue_1484" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedandExpiredWeightedAverageGrantDateFairValue" xlink:label="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedandExpiredWeightedAverageGrantDateFairValue_1486" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_2173" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationArrangementsByShareBasedPaymentAwardNonOptionsIssuedInPeriodWeightedAverageExercisePrice" xlink:label="enlv_ShareBasedCompensationArrangementsByShareBasedPaymentAwardNonOptionsIssuedInPeriodWeightedAverageExercisePrice_1482" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_2173_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightDomain_2173" xlink:to="us-gaap_WarrantMember_1477" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsForfeituresAndExpirations_1485" />
    <link:definitionArc xlink:type="arc" order="18.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_1487" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ClassOfWarrantOrRightTable_1475" xlink:to="us-gaap_ClassOfWarrantOrRightAxis_1476" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_1480" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="us-gaap_ClassOfWarrantOrRightTable_1475" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsIssued_1481" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised_1483" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsExercisedInPeriodWeightedAverageGrantDateFairValue_1484" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedandExpiredWeightedAverageGrantDateFairValue_1486" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1476" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_2173" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightLineItems_1478" xlink:to="enlv_ShareBasedCompensationArrangementsByShareBasedPaymentAwardNonOptionsIssuedInPeriodWeightedAverageExercisePrice_1482" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1476" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_2173_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/PropertyandEquipmentScheduleofPropertyandEquipmentNetDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_LaboratoryEquipmentMember" xlink:label="enlv_LaboratoryEquipmentMember_1429" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OfficeEquipmentMember" xlink:label="us-gaap_OfficeEquipmentMember_1431" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComputerEquipmentMember" xlink:label="us-gaap_ComputerEquipmentMember_1430" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LeaseholdImprovementsMember" xlink:label="us-gaap_LeaseholdImprovementsMember_1432" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1427" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:label="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1435" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems" xlink:label="us-gaap_PropertyPlantAndEquipmentLineItems_1433" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="us-gaap_PropertyPlantAndEquipmentNet_1436" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentGross" xlink:label="us-gaap_PropertyPlantAndEquipmentGross_1434" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable" xlink:label="us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_1426" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428_default" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428" xlink:to="enlv_LaboratoryEquipmentMember_1429" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428" xlink:to="us-gaap_OfficeEquipmentMember_1431" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428" xlink:to="us-gaap_ComputerEquipmentMember_1430" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428" xlink:to="us-gaap_LeaseholdImprovementsMember_1432" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_1426" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1427" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1433" xlink:to="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1435" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1427" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1433" xlink:to="us-gaap_PropertyPlantAndEquipmentNet_1436" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1433" xlink:to="us-gaap_PropertyPlantAndEquipmentGross_1434" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1433" xlink:to="us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_1426" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1427" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_1428_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ShareBasedCompensationScheduleofOptionsGrantedDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember" xlink:label="us-gaap_EmployeeStockOptionMember_1518" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_1523" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriodWeightedAverageExercisePrice_1525" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_1520" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_1530" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_1521" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_1531" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedNumberOfShares" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedNumberOfShares_2278" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedWeightedAverageGrantDateFairValue" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedWeightedAverageGrantDateFairValue_2279" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedNumberOfShares" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedNumberOfShares_2280" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedWeightedAverageGrantDateFairValue" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedWeightedAverageGrantDateFairValue_2281" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedNumberOfShares" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedNumberOfShares_2282" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedWeightedAverageGrantDateFairValue" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedWeightedAverageGrantDateFairValue_2283" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationScheduleofOptionsGrantedDetailsTable" xlink:label="enlv_ShareBasedCompensationScheduleofOptionsGrantedDetailsTable_1516" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedAndExpiredWeightedAverageGrantDateFairValue" xlink:label="enlv_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedAndExpiredWeightedAverageGrantDateFairValue_2344" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionOutstandingWeightedAverageExercisePrice" xlink:label="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionOutstandingWeightedAverageExercisePrice_2368" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionNonvestedNumberOfShares" xlink:label="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionNonvestedNumberOfShares_2370" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis" xlink:label="us-gaap_AwardTypeAxis_1517" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2175" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedAndExpiredNumberOfShares" xlink:label="enlv_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedAndExpiredNumberOfShares_2342" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1522" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriod_1524" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2175_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2175" xlink:to="us-gaap_EmployeeStockOptionMember_1518" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_1523" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriodWeightedAverageExercisePrice_1525" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_1520" />
    <link:definitionArc xlink:type="arc" order="22.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_1530" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_1521" />
    <link:definitionArc xlink:type="arc" order="23.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_1531" />
    <link:definitionArc xlink:type="arc" order="26.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedNumberOfShares_2278" />
    <link:definitionArc xlink:type="arc" order="27.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedWeightedAverageGrantDateFairValue_2279" />
    <link:definitionArc xlink:type="arc" order="28.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedNumberOfShares_2280" />
    <link:definitionArc xlink:type="arc" order="29.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedWeightedAverageGrantDateFairValue_2281" />
    <link:definitionArc xlink:type="arc" order="32.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedNumberOfShares_2282" />
    <link:definitionArc xlink:type="arc" order="34.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedWeightedAverageGrantDateFairValue_2283" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="enlv_ShareBasedCompensationScheduleofOptionsGrantedDetailsTable_1516" />
    <link:definitionArc xlink:type="arc" order="31.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="enlv_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedAndExpiredWeightedAverageGrantDateFairValue_2344" />
    <link:definitionArc xlink:type="arc" order="21.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionOutstandingWeightedAverageExercisePrice_2368" />
    <link:definitionArc xlink:type="arc" order="33.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="enlv_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionNonvestedNumberOfShares_2370" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ShareBasedCompensationScheduleofOptionsGrantedDetailsTable_1516" xlink:to="us-gaap_AwardTypeAxis_1517" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AwardTypeAxis_1517" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2175" />
    <link:definitionArc xlink:type="arc" order="30.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="enlv_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedOptionsForfeitedAndExpiredNumberOfShares_2342" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1522" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1519" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriod_1524" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AwardTypeAxis_1517" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2175_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ShareBasedCompensationDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember" xlink:label="us-gaap_EmployeeStockOptionMember_1827" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_1831" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember" xlink:label="us-gaap_RestrictedStockUnitsRSUMember_1828" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis" xlink:label="us-gaap_AwardTypeAxis_1825" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1829" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExcessStockSharesAuthorized" xlink:label="us-gaap_ExcessStockSharesAuthorized_1833" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_1839" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShareBasedCompensationDetailsTable" xlink:label="enlv_ShareBasedCompensationDetailsTable_1824" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1826" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1830" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1832" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_1840" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpenseNetOfTax" xlink:label="us-gaap_AllocatedShareBasedCompensationExpenseNetOfTax_1842" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1826_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1830_default" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1826" xlink:to="us-gaap_EmployeeStockOptionMember_1827" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1830" xlink:to="us-gaap_CommonStockMember_1831" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1826" xlink:to="us-gaap_RestrictedStockUnitsRSUMember_1828" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ShareBasedCompensationDetailsTable_1824" xlink:to="us-gaap_AwardTypeAxis_1825" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ShareBasedCompensationDetailsTable_1824" xlink:to="us-gaap_StatementClassOfStockAxis_1829" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1832" xlink:to="us-gaap_ExcessStockSharesAuthorized_1833" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1832" xlink:to="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_1839" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1832" xlink:to="enlv_ShareBasedCompensationDetailsTable_1824" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AwardTypeAxis_1825" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1826" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1829" xlink:to="us-gaap_ClassOfStockDomain_1830" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1832" xlink:to="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_1840" />
    <link:definitionArc xlink:type="arc" order="19.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1832" xlink:to="us-gaap_AllocatedShareBasedCompensationExpenseNetOfTax_1842" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AwardTypeAxis_1825" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1826_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1829" xlink:to="us-gaap_ClassOfStockDomain_1830_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ShortTermDepositsScheduleofShortTermDepositsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_ILS" xlink:label="currency_ILS_1352" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherShortTermInvestments" xlink:label="us-gaap_OtherShortTermInvestments_1354" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShortTermDepositsScheduleofShortTermDepositsDetailsTable" xlink:label="enlv_ShortTermDepositsScheduleofShortTermDepositsDetailsTable_1348" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_AllCurrenciesDomain" xlink:label="currency_AllCurrenciesDomain_1350" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CurrencyAxis" xlink:label="srt_CurrencyAxis_1349" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_USD" xlink:label="currency_USD_1351" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfInvestmentsLineItems" xlink:label="us-gaap_ScheduleOfInvestmentsLineItems_1353" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/currency/2026/currency-2026.xsd#currency_AllCurrenciesDomain" xlink:label="currency_AllCurrenciesDomain_1350_default" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="currency_AllCurrenciesDomain_1350" xlink:to="currency_ILS_1352" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfInvestmentsLineItems_1353" xlink:to="us-gaap_OtherShortTermInvestments_1354" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ScheduleOfInvestmentsLineItems_1353" xlink:to="enlv_ShortTermDepositsScheduleofShortTermDepositsDetailsTable_1348" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_CurrencyAxis_1349" xlink:to="currency_AllCurrenciesDomain_1350" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ShortTermDepositsScheduleofShortTermDepositsDetailsTable_1348" xlink:to="srt_CurrencyAxis_1349" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="currency_AllCurrenciesDomain_1350" xlink:to="currency_USD_1351" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_CurrencyAxis_1349" xlink:to="currency_AllCurrenciesDomain_1350_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/EventsSubsequenttotheBalanceSheetDateDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_1862" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1864" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_1866" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventMember" xlink:label="us-gaap_SubsequentEventMember_1868" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2308" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SalesAgreementMember" xlink:label="enlv_SalesAgreementMember_2309" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SecuritiesPurchaseAgreementMember" xlink:label="enlv_SecuritiesPurchaseAgreementMember_2330" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_SeniorSecuredConvertiblePromissoryNoteMember" xlink:label="enlv_SeniorSecuredConvertiblePromissoryNoteMember_2350" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_1865" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_1860" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeDomain" xlink:label="us-gaap_SubsequentEventTypeDomain_2182" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventLineItems" xlink:label="us-gaap_SubsequentEventLineItems_1872" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable" xlink:label="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1861" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock" xlink:label="us-gaap_ProceedsFromIssuanceOfCommonStock_2306" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis" xlink:label="us-gaap_TypeOfArrangementAxis_2307" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_PaymentsForPurchaseOfPrivatePlacement" xlink:label="enlv_PaymentsForPurchaseOfPrivatePlacement_2329" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentPeriodicPayment" xlink:label="us-gaap_DebtInstrumentPeriodicPayment_2343" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateDuringPeriod" xlink:label="us-gaap_DebtInstrumentInterestRateDuringPeriod_2345" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentMaturityDate" xlink:label="us-gaap_DebtInstrumentMaturityDate_2346" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis" xlink:label="us-gaap_DebtInstrumentAxis_2347" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain" xlink:label="us-gaap_DebtInstrumentNameDomain_2348" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentConvertibleConversionPrice1" xlink:label="us-gaap_DebtInstrumentConvertibleConversionPrice1_2351" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_PercentVolumeWeightedAveragePricesOfTheOrdinaryShares" xlink:label="enlv_PercentVolumeWeightedAveragePricesOfTheOrdinaryShares_2352" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_PercentOfPremium" xlink:label="enlv_PercentOfPremium_2353" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_1863" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeAxis" xlink:label="us-gaap_SubsequentEventTypeAxis_1867" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_TradingDays" xlink:label="enlv_TradingDays_2337" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_MarketCapitalization" xlink:label="enlv_MarketCapitalization_2340" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConvertibleNotesPayable" xlink:label="us-gaap_ConvertibleNotesPayable_2341" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_StockIssuedDuringPeriodShareNewIssue" xlink:label="enlv_StockIssuedDuringPeriodShareNewIssue_2381" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1864_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2308_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeDomain" xlink:label="us-gaap_SubsequentEventTypeDomain_2182_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1861_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain" xlink:label="us-gaap_DebtInstrumentNameDomain_2348_default" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1861" xlink:to="us-gaap_CommonStockMember_1862" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_1863" xlink:to="srt_RangeMember_1864" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1864" xlink:to="srt_MaximumMember_1866" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventTypeDomain_2182" xlink:to="us-gaap_SubsequentEventMember_1868" />
    <link:definitionArc xlink:type="arc" order="195.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TypeOfArrangementAxis_2307" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2308" />
    <link:definitionArc xlink:type="arc" order="196.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2308" xlink:to="enlv_SalesAgreementMember_2309" />
    <link:definitionArc xlink:type="arc" order="206.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2308" xlink:to="enlv_SecuritiesPurchaseAgreementMember_2330" />
    <link:definitionArc xlink:type="arc" order="224.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain_2348" xlink:to="enlv_SeniorSecuredConvertiblePromissoryNoteMember_2350" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1864" xlink:to="srt_MinimumMember_1865" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" xlink:to="us-gaap_StatementEquityComponentsAxis_1860" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsequentEventTypeAxis_1867" xlink:to="us-gaap_SubsequentEventTypeDomain_2182" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_1860" xlink:to="us-gaap_EquityComponentDomain_1861" />
    <link:definitionArc xlink:type="arc" order="21.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="us-gaap_ProceedsFromIssuanceOfCommonStock_2306" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" xlink:to="us-gaap_TypeOfArrangementAxis_2307" />
    <link:definitionArc xlink:type="arc" order="23.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_PaymentsForPurchaseOfPrivatePlacement_2329" />
    <link:definitionArc xlink:type="arc" order="27.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="us-gaap_DebtInstrumentPeriodicPayment_2343" />
    <link:definitionArc xlink:type="arc" order="28.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="us-gaap_DebtInstrumentInterestRateDuringPeriod_2345" />
    <link:definitionArc xlink:type="arc" order="29.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="us-gaap_DebtInstrumentMaturityDate_2346" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" xlink:to="us-gaap_DebtInstrumentAxis_2347" />
    <link:definitionArc xlink:type="arc" order="222.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DebtInstrumentAxis_2347" xlink:to="us-gaap_DebtInstrumentNameDomain_2348" />
    <link:definitionArc xlink:type="arc" order="30.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="us-gaap_DebtInstrumentConvertibleConversionPrice1_2351" />
    <link:definitionArc xlink:type="arc" order="31.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_PercentVolumeWeightedAveragePricesOfTheOrdinaryShares_2352" />
    <link:definitionArc xlink:type="arc" order="32.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_PercentOfPremium_2353" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" xlink:to="srt_RangeAxis_1863" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_EventsSubsequenttotheBalanceSheetDateDetailsTable_1859" xlink:to="us-gaap_SubsequentEventTypeAxis_1867" />
    <link:definitionArc xlink:type="arc" order="24.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_TradingDays_2337" />
    <link:definitionArc xlink:type="arc" order="25.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_MarketCapitalization_2340" />
    <link:definitionArc xlink:type="arc" order="26.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="us-gaap_ConvertibleNotesPayable_2341" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1872" xlink:to="enlv_StockIssuedDuringPeriodShareNewIssue_2381" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_1863" xlink:to="srt_RangeMember_1864_default" />
    <link:definitionArc xlink:type="arc" order="195.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TypeOfArrangementAxis_2307" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2308_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsequentEventTypeAxis_1867" xlink:to="us-gaap_SubsequentEventTypeDomain_2182_default" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_1860" xlink:to="us-gaap_EquityComponentDomain_1861_default" />
    <link:definitionArc xlink:type="arc" order="222.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DebtInstrumentAxis_2347" xlink:to="us-gaap_DebtInstrumentNameDomain_2348_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member" xlink:label="us-gaap_FairValueInputsLevel2Member_1644" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member" xlink:label="us-gaap_FairValueInputsLevel3Member_1645" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member" xlink:label="us-gaap_FairValueInputsLevel1Member_1643" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetailsTable" xlink:label="enlv_FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetailsTable_1640" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ShortTermDigitalAssetsFairValueDisclosure" xlink:label="enlv_ShortTermDigitalAssetsFairValueDisclosure_1650" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_DigitalAssetsOptionFairValueDisclosure" xlink:label="enlv_DigitalAssetsOptionFairValueDisclosure_1652" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLiabilitiesCurrent" xlink:label="us-gaap_DerivativeLiabilitiesCurrent_2356" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesFairValueDisclosure" xlink:label="us-gaap_LiabilitiesFairValueDisclosure_2358" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_LongTermDigitalAssetsFairValueDisclosure" xlink:label="enlv_LongTermDigitalAssetsFairValueDisclosure_1651" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsFairValueDisclosure" xlink:label="us-gaap_AssetsFairValueDisclosure_1653" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:label="us-gaap_FairValueByFairValueHierarchyLevelAxis_1641" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642_default" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642" xlink:to="us-gaap_FairValueInputsLevel2Member_1644" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642" xlink:to="us-gaap_FairValueInputsLevel3Member_1645" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642" xlink:to="us-gaap_FairValueInputsLevel1Member_1643" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="enlv_FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetailsTable_1640" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis_1641" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="enlv_ShortTermDigitalAssetsFairValueDisclosure_1650" />
    <link:definitionArc xlink:type="arc" order="15.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="enlv_DigitalAssetsOptionFairValueDisclosure_1652" />
    <link:definitionArc xlink:type="arc" order="17.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="us-gaap_DerivativeLiabilitiesCurrent_2356" />
    <link:definitionArc xlink:type="arc" order="231.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="us-gaap_LiabilitiesFairValueDisclosure_2358" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="enlv_LongTermDigitalAssetsFairValueDisclosure_1651" />
    <link:definitionArc xlink:type="arc" order="16.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_1646" xlink:to="us-gaap_AssetsFairValueDisclosure_1653" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_FairValueHierarchyScheduleofFinancialAssetsMeasuredatFairValueonaRecurringBasisDetailsTable_1640" xlink:to="us-gaap_FairValueByFairValueHierarchyLevelAxis_1641" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis_1641" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_1642_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DerivativeLiabilityScheduleofDerivativeLiabilitiesMeasuredUsingLevel3InputsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member" xlink:label="us-gaap_FairValueInputsLevel3Member_2302" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DefinedBenefitPlanPlanAssetsLevel3ReconciliationTable" xlink:label="us-gaap_DefinedBenefitPlanPlanAssetsLevel3ReconciliationTable_2299" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis" xlink:label="us-gaap_FairValueByFairValueHierarchyLevelAxis_2300" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2301" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems" xlink:label="enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems_2304" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLiabilitiesCurrent" xlink:label="us-gaap_DerivativeLiabilitiesCurrent_2308" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilityGainLossIncludedInEarnings" xlink:label="us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilityGainLossIncludedInEarnings_2324" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_FairValueUponInitialRecognitions" xlink:label="enlv_FairValueUponInitialRecognitions_2325" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilitySettlements" xlink:label="us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilitySettlements_2326" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain" xlink:label="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2301_default" />
    <link:definitionArc xlink:type="arc" order="189.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2301" xlink:to="us-gaap_FairValueInputsLevel3Member_2302" />
    <link:definitionArc xlink:type="arc" order="186.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems_2304" xlink:to="us-gaap_DefinedBenefitPlanPlanAssetsLevel3ReconciliationTable_2299" />
    <link:definitionArc xlink:type="arc" order="187.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DefinedBenefitPlanPlanAssetsLevel3ReconciliationTable_2299" xlink:to="us-gaap_FairValueByFairValueHierarchyLevelAxis_2300" />
    <link:definitionArc xlink:type="arc" order="188.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis_2300" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2301" />
    <link:definitionArc xlink:type="arc" order="11.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems_2304" xlink:to="us-gaap_DerivativeLiabilitiesCurrent_2308" />
    <link:definitionArc xlink:type="arc" order="13.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems_2304" xlink:to="us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilityGainLossIncludedInEarnings_2324" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems_2304" xlink:to="enlv_FairValueUponInitialRecognitions_2325" />
    <link:definitionArc xlink:type="arc" order="14.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_ScheduleOfDerivativeLiabilitiesMeasuredUsingLevel3InputsLineItems_2304" xlink:to="us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilitySettlements_2326" />
    <link:definitionArc xlink:type="arc" order="188.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FairValueByFairValueHierarchyLevelAxis_2300" xlink:to="us-gaap_FairValueMeasurementsFairValueHierarchyDomain_2301_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/ConvertibleDebtDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_NoteMember" xlink:label="enlv_NoteMember_2377" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ConvertibleDebtsTable" xlink:label="enlv_ConvertibleDebtsTable_2221" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtConversionLineItems" xlink:label="us-gaap_DebtConversionLineItems_2223" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount" xlink:label="us-gaap_DebtInstrumentFaceAmount_2224" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfFinancingCosts" xlink:label="us-gaap_AmortizationOfFinancingCosts_2354" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_PercentageOfAdditionalPremium" xlink:label="enlv_PercentageOfAdditionalPremium_2359" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentPeriodicPaymentPrincipal" xlink:label="us-gaap_DebtInstrumentPeriodicPaymentPrincipal_2360" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsOfDebtIssuanceCosts" xlink:label="us-gaap_PaymentsOfDebtIssuanceCosts_2361" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtConversionConvertedInstrumentAmount1" xlink:label="us-gaap_DebtConversionConvertedInstrumentAmount1_2362" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromConvertibleDebt" xlink:label="us-gaap_ProceedsFromConvertibleDebt_2242" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentUnamortizedDiscountPremiumNet" xlink:label="us-gaap_DebtInstrumentUnamortizedDiscountPremiumNet_2258" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentMaturityDate" xlink:label="us-gaap_DebtInstrumentMaturityDate_2374" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis" xlink:label="us-gaap_DebtInstrumentAxis_2375" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain" xlink:label="us-gaap_DebtInstrumentNameDomain_2376" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_IssuanceOfSharesToSettlePrincipalInstallment" xlink:label="enlv_IssuanceOfSharesToSettlePrincipalInstallment_2380" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain" xlink:label="us-gaap_DebtInstrumentNameDomain_2376_default" />
    <link:definitionArc xlink:type="arc" order="242.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain_2376" xlink:to="enlv_NoteMember_2377" />
    <link:definitionArc xlink:type="arc" order="138.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="enlv_ConvertibleDebtsTable_2221" />
    <link:definitionArc xlink:type="arc" order="140.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_DebtInstrumentFaceAmount_2224" />
    <link:definitionArc xlink:type="arc" order="228.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_AmortizationOfFinancingCosts_2354" />
    <link:definitionArc xlink:type="arc" order="232.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="enlv_PercentageOfAdditionalPremium_2359" />
    <link:definitionArc xlink:type="arc" order="233.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_DebtInstrumentPeriodicPaymentPrincipal_2360" />
    <link:definitionArc xlink:type="arc" order="234.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_PaymentsOfDebtIssuanceCosts_2361" />
    <link:definitionArc xlink:type="arc" order="235.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_DebtConversionConvertedInstrumentAmount1_2362" />
    <link:definitionArc xlink:type="arc" order="154.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_ProceedsFromConvertibleDebt_2242" />
    <link:definitionArc xlink:type="arc" order="168.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_DebtInstrumentUnamortizedDiscountPremiumNet_2258" />
    <link:definitionArc xlink:type="arc" order="237.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="us-gaap_DebtInstrumentMaturityDate_2374" />
    <link:definitionArc xlink:type="arc" order="12.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="enlv_ConvertibleDebtsTable_2221" xlink:to="us-gaap_DebtInstrumentAxis_2375" />
    <link:definitionArc xlink:type="arc" order="241.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DebtInstrumentAxis_2375" xlink:to="us-gaap_DebtInstrumentNameDomain_2376" />
    <link:definitionArc xlink:type="arc" order="245.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtConversionLineItems_2223" xlink:to="enlv_IssuanceOfSharesToSettlePrincipalInstallment_2380" />
    <link:definitionArc xlink:type="arc" order="241.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DebtInstrumentAxis_2375" xlink:to="us-gaap_DebtInstrumentNameDomain_2376_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://enlivex.com/20260630/role/DerivativeLiabilityScheduleofValuationAssumptionofDerivativeLiabilityDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputRiskFreeInterestRateMember" xlink:label="us-gaap_MeasurementInputRiskFreeInterestRateMember_2264" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputPriceVolatilityMember" xlink:label="us-gaap_MeasurementInputPriceVolatilityMember_2265" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputExpectedTermMember" xlink:label="us-gaap_MeasurementInputExpectedTermMember_2266" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable" xlink:label="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_2261" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeAxis" xlink:label="us-gaap_MeasurementInputTypeAxis_2262" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain" xlink:label="us-gaap_MeasurementInputTypeDomain_2263" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLiabilityMeasurementInput" xlink:label="us-gaap_DerivativeLiabilityMeasurementInput_2268" />
    <link:loc xlink:type="locator" xlink:href="enlv-20260630.xsd#enlv_ScheduleOfValuationAssumptionOfDerivativeLiabilityLineItems" xlink:label="enlv_ScheduleOfValuationAssumptionOfDerivativeLiabilityLineItems_2273" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain" xlink:label="us-gaap_MeasurementInputTypeDomain_2263_default" />
    <link:definitionArc xlink:type="arc" order="173.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_MeasurementInputTypeDomain_2263" xlink:to="us-gaap_MeasurementInputRiskFreeInterestRateMember_2264" />
    <link:definitionArc xlink:type="arc" order="174.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_MeasurementInputTypeDomain_2263" xlink:to="us-gaap_MeasurementInputPriceVolatilityMember_2265" />
    <link:definitionArc xlink:type="arc" order="175.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_MeasurementInputTypeDomain_2263" xlink:to="us-gaap_MeasurementInputExpectedTermMember_2266" />
    <link:definitionArc xlink:type="arc" order="170.00000" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="enlv_ScheduleOfValuationAssumptionOfDerivativeLiabilityLineItems_2273" xlink:to="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_2261" />
    <link:definitionArc xlink:type="arc" order="171.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_2261" xlink:to="us-gaap_MeasurementInputTypeAxis_2262" />
    <link:definitionArc xlink:type="arc" order="172.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_MeasurementInputTypeAxis_2262" xlink:to="us-gaap_MeasurementInputTypeDomain_2263" />
    <link:definitionArc xlink:type="arc" order="10.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="enlv_ScheduleOfValuationAssumptionOfDerivativeLiabilityLineItems_2273" xlink:to="us-gaap_DerivativeLiabilityMeasurementInput_2268" />
    <link:definitionArc xlink:type="arc" order="172.00000" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_MeasurementInputTypeAxis_2262" xlink:to="us-gaap_MeasurementInputTypeDomain_2263_default" />
  </link:definitionLink>
</link:linkbase>
