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          <filerCik>0001932193</filerCik>
          <filerCcc>XXXXXXXX</filerCcc>
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        <fileNumber>020-34040</fileNumber>
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      <period>12-31-2025</period>
      <liveTestFlag>LIVE</liveTestFlag>
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        <returnCopyFlag>true</returnCopyFlag>
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        <nameOfIssuer>RS BioTherapeutics, Inc.</nameOfIssuer>
        <legalStatus>
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          <jurisdictionOrganization>DE</jurisdictionOrganization>
          <dateIncorporation>02-08-2021</dateIncorporation>
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        <issuerAddress>
          <com:street1>157 Baltimore St. Suite 140</com:street1>
          <com:city>Cumberland</com:city>
          <com:stateOrCountry>MD</com:stateOrCountry>
          <com:zipCode>21502</com:zipCode>
        </issuerAddress>
        <issuerWebsite>http://rsbiotherapeutics.com</issuerWebsite>
      </issuerInfo>
      <isCoIssuer>N</isCoIssuer>
    </issuerInformation>
    <offeringInformation>
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    <annualReportDisclosureRequirements>
      <currentEmployees>2</currentEmployees>
      <totalAssetMostRecentFiscalYear>13807.63</totalAssetMostRecentFiscalYear>
      <totalAssetPriorFiscalYear>40617.00</totalAssetPriorFiscalYear>
      <cashEquiMostRecentFiscalYear>9302.63</cashEquiMostRecentFiscalYear>
      <cashEquiPriorFiscalYear>14158.00</cashEquiPriorFiscalYear>
      <actReceivedMostRecentFiscalYear>0.00</actReceivedMostRecentFiscalYear>
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      <shortTermDebtMostRecentFiscalYear>1249122.90</shortTermDebtMostRecentFiscalYear>
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      <longTermDebtMostRecentFiscalYear>188555.05</longTermDebtMostRecentFiscalYear>
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    <signatureInfo>
      <issuerSignature>
        <issuer>RS BioTherapeutics, Inc.</issuer>
        <issuerSignature>Justin Molignoni</issuerSignature>
        <issuerTitle>Chairman of the Board, Chief Strategy Officer</issuerTitle>
      </issuerSignature>
      <signaturePersons>
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          <personSignature>Justin Molignoni</personSignature>
          <personTitle>Chairman of the Board, Chief Strategy Officer</personTitle>
          <signatureDate>09-22-2026</signatureDate>
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      </signaturePersons>
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