CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM
We hereby consent to the incorporation by reference in this Registration Statement on Form S-3 of Nautilus Biotechnology, Inc. of our report dated February 26, 2026 relating to the financial statements, which appears in Nautilus Biotechnology, Inc.'s Annual Report on Form 10-K for the year ended December 31, 2025. We also consent to the reference to us under the heading "Experts" in such Registration Statement.
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| /s/ PricewaterhouseCoopers LLP |
San Jose, California
September 11, 2026