<?xml version="1.0" encoding="US-ASCII" standalone="no"?>
    <!-- Field: Doc-Info; Name: Generator; Value: GoFiler Complete; Version: 6.5a -->
    <!-- Field: Doc-Info; Name: VendorURI; Value: https://www.novaworks.com -->
    <!-- Field: Doc-Info; Name: Status; Value: 0x00000000 -->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:xbrli="http://www.xbrl.org/2003/instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#Cover" roleURI="http://pulsenmore.com/role/Cover" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#InterimStatementsOfFinancialPosition" roleURI="http://pulsenmore.com/role/InterimStatementsOfFinancialPosition" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#InterimStatementsOfComprehensiveLoss" roleURI="http://pulsenmore.com/role/InterimStatementsOfComprehensiveLoss" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#InterimStatementsOfComprehensiveLossParenthetical" roleURI="http://pulsenmore.com/role/InterimStatementsOfComprehensiveLossParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#InterimStatementsOfChangesInEquity" roleURI="http://pulsenmore.com/role/InterimStatementsOfChangesInEquity" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#InterimStatementsOfCashFlows" roleURI="http://pulsenmore.com/role/InterimStatementsOfCashFlows" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#GeneralInformation" roleURI="http://pulsenmore.com/role/GeneralInformation" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatements" roleURI="http://pulsenmore.com/role/BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatements" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#SignificantAccountingPolicies" roleURI="http://pulsenmore.com/role/SignificantAccountingPolicies" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#Financings" roleURI="http://pulsenmore.com/role/Financings" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#FinancialInstrumentsAndFinancialRiskManagement" roleURI="http://pulsenmore.com/role/FinancialInstrumentsAndFinancialRiskManagement" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#CommitmentsAndContingentLiabilities" roleURI="http://pulsenmore.com/role/CommitmentsAndContingentLiabilities" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#SubsequentEvents" roleURI="http://pulsenmore.com/role/SubsequentEvents" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatementsPolicies" roleURI="http://pulsenmore.com/role/BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatementsPolicies" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#FinancialInstrumentsAndFinancialRiskManagementTables" roleURI="http://pulsenmore.com/role/FinancialInstrumentsAndFinancialRiskManagementTables" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatementsDetailsNarrative" roleURI="http://pulsenmore.com/role/BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatementsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#FinancingsDetailsNarrative" roleURI="http://pulsenmore.com/role/FinancingsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#ScheduleOfFinancialInstrumentsAndFinancialRiskManagementDetails" roleURI="http://pulsenmore.com/role/ScheduleOfFinancialInstrumentsAndFinancialRiskManagementDetails" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#ScheduleOfChangesInFinancialInstrumentsDetails" roleURI="http://pulsenmore.com/role/ScheduleOfChangesInFinancialInstrumentsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#FinancialInstrumentsAndFinancialRiskManagementDetailsNarrative" roleURI="http://pulsenmore.com/role/FinancialInstrumentsAndFinancialRiskManagementDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#CommitmentsAndContingentLiabilitiesDetailsNarrative" roleURI="http://pulsenmore.com/role/CommitmentsAndContingentLiabilitiesDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="plsm-20260630.xsd#SubsequentEventsDetailsNarrative" roleURI="http://pulsenmore.com/role/SubsequentEventsDetailsNarrative" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#notAll" arcroleURI="http://xbrl.org/int/dim/arcrole/notAll" />
    <link:arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/Cover" xlink:title="00000001 - Document - Cover" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/InterimStatementsOfFinancialPosition" xlink:title="00000002 - Statement - Condensed Consolidated Interim Statements of Financial Position (Unaudited)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/InterimStatementsOfComprehensiveLoss" xlink:title="00000003 - Statement - Condensed Consolidated Interim Statements of Comprehensive Loss (Unaudited)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/InterimStatementsOfComprehensiveLossParenthetical" xlink:title="00000004 - Statement - Condensed Consolidated Interim Statements of Comprehensive Loss (Unaudited) (Parenthetical)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/InterimStatementsOfChangesInEquity" xlink:title="00000005 - Statement - Condensed Consolidated Interim Statements of Changes in Equity (Unaudited)">
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IfrsStatementLineItems" xlink:label="loc_PLSMIfrsStatementLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_StatementOfChangesInEquityTable" xlink:label="loc_ifrs-fullStatementOfChangesInEquityTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullStatementOfChangesInEquityTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityAxis" xlink:label="loc_ifrs-fullComponentsOfEquityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullStatementOfChangesInEquityTable" xlink:to="loc_ifrs-fullComponentsOfEquityAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityDomain" xlink:label="loc_ifrs-fullComponentsOfEquityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullComponentsOfEquityAxis" xlink:to="loc_ifrs-fullComponentsOfEquityDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityDomain" xlink:label="loc_ifrs-fullComponentsOfEquityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullComponentsOfEquityAxis" xlink:to="loc_ifrs-fullComponentsOfEquityDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OrdinarySharesMember" xlink:label="loc_ifrs-fullOrdinarySharesMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullComponentsOfEquityDomain" xlink:to="loc_ifrs-fullOrdinarySharesMember_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_SharePremiumMember" xlink:label="loc_ifrs-fullSharePremiumMember_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullComponentsOfEquityDomain" xlink:to="loc_ifrs-fullSharePremiumMember_10" xlink:type="arc" order="11" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CapitalReserveMember" xlink:label="loc_ifrs-fullCapitalReserveMember_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullComponentsOfEquityDomain" xlink:to="loc_ifrs-fullCapitalReserveMember_20" xlink:type="arc" order="21" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_RetainedEarningsMember" xlink:label="loc_ifrs-fullRetainedEarningsMember_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullComponentsOfEquityDomain" xlink:to="loc_ifrs-fullRetainedEarningsMember_30" xlink:type="arc" order="31" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Equity" xlink:label="loc_ifrs-fullEquity_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullEquity_60" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProfitLoss" xlink:label="loc_ifrs-fullProfitLoss_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullProfitLoss_60" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughSharebasedPaymentTransactions" xlink:label="loc_ifrs-fullIncreaseDecreaseThroughSharebasedPaymentTransactions_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullIncreaseDecreaseThroughSharebasedPaymentTransactions_60" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_IncreaseDecreaseThroughExerciseOfOptions" xlink:label="loc_ifrs-fullIncreaseDecreaseThroughExerciseOfOptions_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullIncreaseDecreaseThroughExerciseOfOptions_60" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IncreaseDecreaseThroughExpirationOfOptions" xlink:label="loc_PLSMIncreaseDecreaseThroughExpirationOfOptions_60" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMIncreaseDecreaseThroughExpirationOfOptions_60" xlink:type="arc" order="5" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/InterimStatementsOfCashFlows" xlink:title="00000006 - Statement - Condensed Consolidated Interim Statements of Cash Flows" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/GeneralInformation" xlink:title="999007 - Disclosure - GENERAL INFORMATION" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatements" xlink:title="999008 - Disclosure - BASIS OF PREPARATION OF CONDENSED CONSOLIDATED INTERIM FINANCIAL STATEMENTS" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/SignificantAccountingPolicies" xlink:title="999009 - Disclosure - SIGNIFICANT ACCOUNTING POLICIES" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/Financings" xlink:title="999010 - Disclosure - FINANCINGS" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/FinancialInstrumentsAndFinancialRiskManagement" xlink:title="999011 - Disclosure - FINANCIAL INSTRUMENTS AND FINANCIAL RISK MANAGEMENT" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/CommitmentsAndContingentLiabilities" xlink:title="999012 - Disclosure - COMMITMENTS AND CONTINGENT LIABILITIES" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/SubsequentEvents" xlink:title="999013 - Disclosure - SUBSEQUENT EVENTS" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatementsPolicies" xlink:title="999014 - Disclosure - BASIS OF PREPARATION OF CONDENSED CONSOLIDATED INTERIM FINANCIAL STATEMENTS (Policies)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/FinancialInstrumentsAndFinancialRiskManagementTables" xlink:title="999015 - Disclosure - FINANCIAL INSTRUMENTS AND FINANCIAL RISK MANAGEMENT (Tables)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/BasisOfPreparationOfCondensedConsolidatedInterimFinancialStatementsDetailsNarrative" xlink:title="999016 - Disclosure - BASIS OF PREPARATION OF CONDENSED CONSOLIDATED INTERIM FINANCIAL STATEMENTS (Details Narrative)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/FinancingsDetailsNarrative" xlink:title="999017 - Disclosure - FINANCINGS (Details Narrative)">
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IfrsStatementLineItems" xlink:label="loc_PLSMIfrsStatementLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable" xlink:label="loc_ifrs-fullDisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullDisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfContractsAxis" xlink:label="loc_ifrs-fullTypesOfContractsAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable" xlink:to="loc_ifrs-fullTypesOfContractsAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfContractsDomain" xlink:label="loc_ifrs-fullTypesOfContractsDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullTypesOfContractsAxis" xlink:to="loc_ifrs-fullTypesOfContractsDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfContractsDomain" xlink:label="loc_ifrs-fullTypesOfContractsDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullTypesOfContractsAxis" xlink:to="loc_ifrs-fullTypesOfContractsDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_SecuritiesPurchaseAgreementMember" xlink:label="loc_PLSMSecuritiesPurchaseAgreementMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullTypesOfContractsDomain" xlink:to="loc_PLSMSecuritiesPurchaseAgreementMember_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityAxis" xlink:label="loc_ifrs-fullComponentsOfEquityAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfDisaggregationOfRevenueFromContractsWithCustomersTable" xlink:to="loc_ifrs-fullComponentsOfEquityAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityDomain" xlink:label="loc_ifrs-fullComponentsOfEquityDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullComponentsOfEquityAxis" xlink:to="loc_ifrs-fullComponentsOfEquityDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ComponentsOfEquityDomain" xlink:label="loc_ifrs-fullComponentsOfEquityDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullComponentsOfEquityAxis" xlink:to="loc_ifrs-fullComponentsOfEquityDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_PrefundedWarrantsMember" xlink:label="loc_PLSMPrefundedWarrantsMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullComponentsOfEquityDomain" xlink:to="loc_PLSMPrefundedWarrantsMember_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WarrantsMember" xlink:label="loc_ifrs-fullWarrantsMember_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullComponentsOfEquityDomain" xlink:to="loc_ifrs-fullWarrantsMember_10" xlink:type="arc" order="12" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NumberOfSharesIssued" xlink:label="loc_ifrs-fullNumberOfSharesIssued_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullNumberOfSharesIssued_80" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ParValuePerShare" xlink:label="loc_ifrs-fullParValuePerShare_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullParValuePerShare_80" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromIssuingShares" xlink:label="loc_ifrs-fullProceedsFromIssuingShares_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullProceedsFromIssuingShares_80" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ShareIssueRelatedCost" xlink:label="loc_ifrs-fullShareIssueRelatedCost_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullShareIssueRelatedCost_80" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WeightedAverageSharePrice2019" xlink:label="loc_ifrs-fullWeightedAverageSharePrice2019_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullWeightedAverageSharePrice2019_80" xlink:type="arc" order="4" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_FinanceCosts" xlink:label="loc_ifrs-fullFinanceCosts_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullFinanceCosts_80" xlink:type="arc" order="5" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_DeferredLoss" xlink:label="loc_PLSMDeferredLoss_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMDeferredLoss_80" xlink:type="arc" order="6" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_WeightedAverageRemainingContractualLifeOfOutstandingWarrants" xlink:label="loc_PLSMWeightedAverageRemainingContractualLifeOfOutstandingWarrants_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMWeightedAverageRemainingContractualLifeOfOutstandingWarrants_80" xlink:type="arc" order="7" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_OtherExpenseByNature" xlink:label="loc_ifrs-fullOtherExpenseByNature_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullOtherExpenseByNature_80" xlink:type="arc" order="8" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_ShareIssueRelatedCostUnpaid" xlink:label="loc_PLSMShareIssueRelatedCostUnpaid_80" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMShareIssueRelatedCostUnpaid_80" xlink:type="arc" order="9" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/ScheduleOfFinancialInstrumentsAndFinancialRiskManagementDetails" xlink:title="999018 - Disclosure - SCHEDULE OF FINANCIAL INSTRUMENTS AND FINANCIAL RISK MANAGEMENT (Details)">
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IfrsStatementLineItems" xlink:label="loc_PLSMIfrsStatementLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfFairValueMeasurementOfAssetsTable" xlink:label="loc_ifrs-fullDisclosureOfFairValueMeasurementOfAssetsTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullDisclosureOfFairValueMeasurementOfAssetsTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyAxis" xlink:label="loc_ifrs-fullLevelsOfFairValueHierarchyAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfFairValueMeasurementOfAssetsTable" xlink:to="loc_ifrs-fullLevelsOfFairValueHierarchyAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain" xlink:label="loc_ifrs-fullLevelsOfFairValueHierarchyDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullLevelsOfFairValueHierarchyAxis" xlink:to="loc_ifrs-fullLevelsOfFairValueHierarchyDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_LevelsOfFairValueHierarchyDomain" xlink:label="loc_ifrs-fullLevelsOfFairValueHierarchyDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullLevelsOfFairValueHierarchyAxis" xlink:to="loc_ifrs-fullLevelsOfFairValueHierarchyDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Level2OfFairValueHierarchyMember" xlink:label="loc_ifrs-fullLevel2OfFairValueHierarchyMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullLevelsOfFairValueHierarchyDomain" xlink:to="loc_ifrs-fullLevel2OfFairValueHierarchyMember_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_Level3OfFairValueHierarchyMember" xlink:label="loc_ifrs-fullLevel3OfFairValueHierarchyMember_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullLevelsOfFairValueHierarchyDomain" xlink:to="loc_ifrs-fullLevel3OfFairValueHierarchyMember_10" xlink:type="arc" order="11" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsAxis" xlink:label="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfFairValueMeasurementOfAssetsTable" xlink:to="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsDomain" xlink:label="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" xlink:to="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsDomain" xlink:label="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" xlink:to="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_PrefundedWarrantsMember" xlink:label="loc_PLSMPrefundedWarrantsMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" xlink:to="loc_PLSMPrefundedWarrantsMember_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WarrantsMember" xlink:label="loc_ifrs-fullWarrantsMember_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" xlink:to="loc_ifrs-fullWarrantsMember_30" xlink:type="arc" order="32" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CurrentWarrantLiability" xlink:label="loc_ifrs-fullCurrentWarrantLiability_100" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullCurrentWarrantLiability_100" xlink:type="arc" order="0" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/ScheduleOfChangesInFinancialInstrumentsDetails" xlink:title="999019 - Disclosure - SCHEDULE OF CHANGES IN THE FINANCIAL INSTRUMENTS (Details)" />
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/FinancialInstrumentsAndFinancialRiskManagementDetailsNarrative" xlink:title="999020 - Disclosure - FINANCIAL INSTRUMENTS AND FINANCIAL RISK MANAGEMENT (Details Narrative)">
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IfrsStatementLineItems" xlink:label="loc_PLSMIfrsStatementLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfInstrumentsWithPotentialFutureDilutiveEffectNotIncludedInCalculationOfDilutedEarningsPerShareTable" xlink:label="loc_ifrs-fullDisclosureOfInstrumentsWithPotentialFutureDilutiveEffectNotIncludedInCalculationOfDilutedEarningsPerShareTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullDisclosureOfInstrumentsWithPotentialFutureDilutiveEffectNotIncludedInCalculationOfDilutedEarningsPerShareTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsAxis" xlink:label="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfInstrumentsWithPotentialFutureDilutiveEffectNotIncludedInCalculationOfDilutedEarningsPerShareTable" xlink:to="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsDomain" xlink:label="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" xlink:to="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfAntidilutiveInstrumentsDomain" xlink:label="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsAxis" xlink:to="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_WarrantsMember" xlink:label="loc_ifrs-fullWarrantsMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullTypesOfAntidilutiveInstrumentsDomain" xlink:to="loc_ifrs-fullWarrantsMember_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_WarrantsSharePrice" xlink:label="loc_PLSMWarrantsSharePrice_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMWarrantsSharePrice_30" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_WarrantsExercisePrice" xlink:label="loc_PLSMWarrantsExercisePrice_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMWarrantsExercisePrice_30" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_DescriptionOfExpectedVolatilityWarrantsGranted" xlink:label="loc_PLSMDescriptionOfExpectedVolatilityWarrantsGranted_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMDescriptionOfExpectedVolatilityWarrantsGranted_30" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_DescriptionOfRiskFreeInterestRateWarrantsGranted" xlink:label="loc_PLSMDescriptionOfRiskFreeInterestRateWarrantsGranted_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMDescriptionOfRiskFreeInterestRateWarrantsGranted_30" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_WarrantsExpectedLife" xlink:label="loc_PLSMWarrantsExpectedLife_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_PLSMWarrantsExpectedLife_30" xlink:type="arc" order="4" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/CommitmentsAndContingentLiabilitiesDetailsNarrative" xlink:title="999021 - Disclosure - COMMITMENTS AND CONTINGENT LIABILITIES (Details Narrative)">
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IfrsStatementLineItems" xlink:label="loc_PLSMIfrsStatementLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:label="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis" xlink:label="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:to="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain" xlink:label="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" xlink:to="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain" xlink:label="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" xlink:to="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_NonadjustingEventMember" xlink:label="loc_PLSMNonadjustingEventMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain" xlink:to="loc_PLSMNonadjustingEventMember_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NameOfAcquiree" xlink:label="loc_ifrs-fullNameOfAcquiree_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullNameOfAcquiree_30" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_AcquisitiondateFairValueOfTotalConsiderationTransferred" xlink:label="loc_ifrs-fullAcquisitiondateFairValueOfTotalConsiderationTransferred_30" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullAcquisitiondateFairValueOfTotalConsiderationTransferred_30" xlink:type="arc" order="1" />
    </link:definitionLink>
    <link:definitionLink xlink:type="extended" xlink:role="http://pulsenmore.com/role/SubsequentEventsDetailsNarrative" xlink:title="999022 - Disclosure - SUBSEQUENT EVENTS (Details Narrative)">
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IfrsStatementLineItems" xlink:label="loc_PLSMIfrsStatementLineItems" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_DisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:label="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xbrldt:contextElement="segment" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:type="arc" order="10" xbrldt:closed="true" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodAxis" xlink:label="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:to="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain" xlink:label="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" xlink:to="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_NonadjustingEventsAfterReportingPeriodDomain" xlink:label="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodAxis" xlink:to="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_NonadjustingEventMember" xlink:label="loc_PLSMNonadjustingEventMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullNonadjustingEventsAfterReportingPeriodDomain" xlink:to="loc_PLSMNonadjustingEventMember_0" xlink:type="arc" order="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfContractsAxis" xlink:label="loc_ifrs-fullTypesOfContractsAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:to="loc_ifrs-fullTypesOfContractsAxis" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfContractsDomain" xlink:label="loc_ifrs-fullTypesOfContractsDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullTypesOfContractsAxis" xlink:to="loc_ifrs-fullTypesOfContractsDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_TypesOfContractsDomain" xlink:label="loc_ifrs-fullTypesOfContractsDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullTypesOfContractsAxis" xlink:to="loc_ifrs-fullTypesOfContractsDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_HealthcareAIRegulatorySandboxProgramMember" xlink:label="loc_PLSMHealthcareAIRegulatorySandboxProgramMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullTypesOfContractsDomain" xlink:to="loc_PLSMHealthcareAIRegulatorySandboxProgramMember_0" xlink:type="arc" order="2" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_SmartScanAIProgramMember" xlink:label="loc_PLSMSmartScanAIProgramMember_20" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullTypesOfContractsDomain" xlink:to="loc_PLSMSmartScanAIProgramMember_20" xlink:type="arc" order="23" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesAxis" xlink:label="loc_ifrs-fullCategoriesOfRelatedPartiesAxis" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_ifrs-fullDisclosureOfNonadjustingEventsAfterReportingPeriodTable" xlink:to="loc_ifrs-fullCategoriesOfRelatedPartiesAxis" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain" xlink:label="loc_ifrs-fullCategoriesOfRelatedPartiesDomain" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_ifrs-fullCategoriesOfRelatedPartiesAxis" xlink:to="loc_ifrs-fullCategoriesOfRelatedPartiesDomain" xlink:type="arc" order="20" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_CategoriesOfRelatedPartiesDomain" xlink:label="loc_ifrs-fullCategoriesOfRelatedPartiesDomain_10" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xbrldt:contextElement="segment" xlink:from="loc_ifrs-fullCategoriesOfRelatedPartiesAxis" xlink:to="loc_ifrs-fullCategoriesOfRelatedPartiesDomain_10" xlink:type="arc" order="30" />
      <link:loc xlink:type="locator" xlink:href="plsm-20260630.xsd#PLSM_IsraelInnovationAuthorityMember" xlink:label="loc_PLSMIsraelInnovationAuthorityMember_0" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ifrs-fullCategoriesOfRelatedPartiesDomain" xlink:to="loc_PLSMIsraelInnovationAuthorityMember_0" xlink:type="arc" order="3" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_GovernmentGrants" xlink:label="loc_ifrs-fullGovernmentGrants_40" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullGovernmentGrants_40" xlink:type="arc" order="0" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.ifrs.org/taxonomy/2025-03-27/full_ifrs/full_ifrs-cor_2025-03-27.xsd#ifrs-full_ProceedsFromGovernmentGrantsClassifiedAsFinancingActivities" xlink:label="loc_ifrs-fullProceedsFromGovernmentGrantsClassifiedAsFinancingActivities_40" />
      <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_PLSMIfrsStatementLineItems" xlink:to="loc_ifrs-fullProceedsFromGovernmentGrantsClassifiedAsFinancingActivities_40" xlink:type="arc" order="1" />
    </link:definitionLink>
</link:linkbase>
