<?xml version="1.0" encoding="UTF-8"?>
<!--Generated by Broadridge Transform (tm) - http://www.broadridge.com-->
<!--Created: Mon Aug 31 15:05:14 UTC 2026-->
<edgarSubmission xsi:schemaLocation="http://www.sec.gov/edgar/npx eis_NPX_Filer.xsd" xmlns="http://www.sec.gov/edgar/npx" xmlns:common="http://www.sec.gov/edgar/common" xmlns:sccommon="http://www.sec.gov/edgar/sccommon" xmlns:iso="http://www.sec.gov/edgar/iso_statecodes" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
  <headerData>
    <submissionType>N-PX</submissionType>
    <filerInfo>
      <registrantType>IM</registrantType>
      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <issuerCredentials>
          <cik>0001179392</cik>
          <ccc>XXXXXXXX</ccc>
        </issuerCredentials>
      </filer>
      <periodOfReport>06/30/2026</periodOfReport>
    </filerInfo>
  </headerData>
  <formData>
    <coverPage>
      <yearOrQuarter>YEAR</yearOrQuarter>
      <reportCalendarYear>2026</reportCalendarYear>
      <reportingPerson>
        <name>TWO SIGMA INVESTMENTS, LP</name>
        <phoneNumber>212-625-5700</phoneNumber>
        <address>
          <common:street1>100 Avenue of the Americas</common:street1>
          <common:street2>16th Floor</common:street2>
          <common:city>New York</common:city>
          <common:stateOrCountry>NY</common:stateOrCountry>
          <common:zipCode>10013</common:zipCode>
        </address>
      </reportingPerson>
      <agentForService>
        <name>Willkie Farr &amp; Gallagher LLP</name>
        <address>
          <common:street1>787 Seventh Avenue</common:street1>
          <common:city>New York</common:city>
          <common:stateOrCountry>NY</common:stateOrCountry>
          <common:zipCode>10019</common:zipCode>
        </address>
      </agentForService>
      <reportInfo>
        <reportType>INSTITUTIONAL MANAGER COMBINATION REPORT</reportType>
        <confidentialTreatment>N</confidentialTreatment>
      </reportInfo>
      <fileNumber>028-10385</fileNumber>
      <reportingCrdNumber>137137</reportingCrdNumber>
      <reportingSecFileNumber>801-70476</reportingSecFileNumber>
      <leiNumber>0M6M8M9HXLW8D3AU0N32</leiNumber>
      <otherManagersInfo>
        <otherManager>
          <icaOr13FFileNumber>811-22743</icaOr13FFileNumber>
          <leiNumberOM>549300C1ND700M70DR68</leiNumberOM>
          <managerName>Blackstone Alternative Investment Funds</managerName>
        </otherManager>
      </otherManagersInfo>
      <explanatoryInformation>
        <explanatoryChoice>Y</explanatoryChoice>
        <explanatoryNotes>This Form N-PX Annual Report of Proxy Voting Record (the "Report") is being filed by Two Sigma Investments, LP ("TSI") jointly with its affiliate, Two Sigma Advisers, LP ("TSA"). The proxy votes for which TSI and TSA exercised voting power during the reporting period are reported separately in this Report. As of January 1, 2026, TSI manages all of the clients that were previously managed by TSA, and TSA no longer provides services to any clients, manages any client assets, or exercises voting power over any securities involving reportable voting matters.</explanatoryNotes>
      </explanatoryInformation>
    </coverPage>
    <summaryPage>
      <otherIncludedManagersCount>1</otherIncludedManagersCount>
      <otherManagers2>
        <investmentManagers>
          <serialNo>1</serialNo>
          <form13FFileNumber>028-14367</form13FFileNumber>
          <crdNumber>000152254</crdNumber>
          <secFileNumber>801-71110</secFileNumber>
          <leiNumber>RR8XXLLT9L8B1RHYI071</leiNumber>
          <name>Two Sigma Advisers, LP</name>
        </investmentManagers>
      </otherManagers2>
    </summaryPage>
    <signaturePage>
      <reportingPerson>TWO SIGMA INVESTMENTS, LP</reportingPerson>
      <txSignature>James G. Hein, Jr.</txSignature>
      <txPrintedSignature>James G. Hein, Jr.</txPrintedSignature>
      <txTitle>Authorized Signatory</txTitle>
      <txAsOfDate>08/31/2026</txAsOfDate>
    </signaturePage>
  </formData>
</edgarSubmission>
