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        <fairValLevel>1</fairValLevel>
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        <name>Amazon.com, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Howmet Aerospace, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <fairValLevel>1</fairValLevel>
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        <fairValLevel>2</fairValLevel>
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        <name>Bullish</name>
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        <fairValLevel>1</fairValLevel>
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        <fairValLevel>2</fairValLevel>
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        <name>Nebius Group NV</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Argenx SE</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Revolution Medicines, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Flutter Entertainment plc</name>
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        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
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        <name>Lumentum Holdings, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Marvell Technology, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Nexstar Media Group, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <fairValLevel>1</fairValLevel>
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        <name>Vertiv Holdings Co.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Builders FirstSource, Inc.</name>
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        <name>Datadog, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Meta Platforms, Inc.</name>
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        <name>Capital One Financial Corp.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Vaxcyte, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Hong Kong Exchanges &amp; Clearing Ltd.</name>
        <lei>213800YTVSXYQN17BW16</lei>
        <title>Hong Kong Exchanges &amp; Clearing Ltd.</title>
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        <invCountry>HK</invCountry>
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        <fairValLevel>2</fairValLevel>
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      </invstOrSec>
      <invstOrSec>
        <name>Blackstone, Inc.</name>
        <lei>5299004LW4QWGZUB8Y96</lei>
        <title>Blackstone, Inc.</title>
        <cusip>09260D107</cusip>
        <identifiers>
          <isin value="US09260D1072"/>
        </identifiers>
        <balance>360011.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>42362494.37000000</valUSD>
        <pctVal>0.771952914552</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Anterix, Inc.</name>
        <lei>549300QIX2QHFGIEQG40</lei>
        <title>Anterix, Inc.</title>
        <cusip>03676C100</cusip>
        <identifiers>
          <isin value="US03676C1009"/>
        </identifiers>
        <balance>634831.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>65349503.14000000</valUSD>
        <pctVal>1.190834962947</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Surgery Partners, Inc.</name>
        <lei>549300LC82HG33S19I03</lei>
        <title>Surgery Partners, Inc.</title>
        <cusip>86881A100</cusip>
        <identifiers>
          <isin value="US86881A1007"/>
        </identifiers>
        <balance>13258236.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>222738364.80000000</valUSD>
        <pctVal>4.058862265952</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Inhouse Asset ID" value="ABH9984U2"/>
        </identifiers>
        <balance>278040596.40420000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>278040579.91000000</valUSD>
        <pctVal>5.066609962830</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>N/A</name>
        <lei>N/A</lei>
        <title>Oracle Corp.</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Inhouse Asset ID" value="453875JI"/>
        </identifiers>
        <balance>-31000000.00000000</balance>
        <units>NC</units>
        <curCd>USD</curCd>
        <valUSD>-2419453.58000000</valUSD>
        <pctVal>-0.04408862769</pctVal>
        <payoffProfile>N/A</payoffProfile>
        <assetCat>DE</assetCat>
        <issuerConditional desc="Option" issuerCat="OTHER"/>
        <invCountry>GB</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <derivativeInfo>
          <optionSwaptionWarrantDeriv derivCat="OPT">
            <counterparties>
              <counterpartyName>GOLDMAN SACHS INTERNATIONAL</counterpartyName>
              <counterpartyLei>W22LROWP2IHZNBB6K528</counterpartyLei>
            </counterparties>
            <putOrCall>Put</putOrCall>
            <writtenOrPur>Written</writtenOrPur>
            <descRefInstrmnt>
              <otherRefInst>
                <issuerName>Oracle Corp.</issuerName>
                <issueTitle>Oracle Corp.</issueTitle>
                <identifiers>
                  <cusip value="68389X105"/>
                </identifiers>
              </otherRefInst>
            </descRefInstrmnt>
            <shareNo>1.00000000</shareNo>
            <exercisePrice>155.00000000</exercisePrice>
            <exercisePriceCurCd>USD</exercisePriceCurCd>
            <expDt>2026-07-17</expDt>
            <delta>XXXX</delta>
            <unrealizedAppr>-1631453.58000000</unrealizedAppr>
          </optionSwaptionWarrantDeriv>
        </derivativeInfo>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Arxis, Inc.</name>
        <lei>N/A</lei>
        <title>Arxis, Inc., Class A</title>
        <cusip>04339D105</cusip>
        <identifiers>
          <isin value="US04339D1054"/>
        </identifiers>
        <balance>1937365.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>89390021.10000000</valUSD>
        <pctVal>1.628914641270</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Wingstop, Inc.</name>
        <lei>549300SSH3F8HPIM3Q47</lei>
        <title>Wingstop, Inc.</title>
        <cusip>974155103</cusip>
        <identifiers>
          <isin value="US9741551033"/>
        </identifiers>
        <balance>582188.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>100957221.08000000</valUSD>
        <pctVal>1.839698587555</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Core &amp; Main, Inc.</name>
        <lei>N/A</lei>
        <title>Core &amp; Main, Inc., Class A</title>
        <cusip>21874C102</cusip>
        <identifiers>
          <isin value="US21874C1027"/>
        </identifiers>
        <balance>1704337.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>82234260.25000000</valUSD>
        <pctVal>1.498518390385</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Cullen</name>
        <lei>529900V2E9G9AY665O34</lei>
        <title>Cullen</title>
        <cusip>229899109</cusip>
        <identifiers>
          <isin value="US2298991090"/>
        </identifiers>
        <balance>582776.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>90050547.52000000</valUSD>
        <pctVal>1.640951120770</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Western Alliance Bancorp</name>
        <lei>5493003VJXZ5JXT9S762</lei>
        <title>Western Alliance Bancorp</title>
        <cusip>957638109</cusip>
        <identifiers>
          <isin value="US9576381092"/>
        </identifiers>
        <balance>1193676.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>98120167.20000000</valUSD>
        <pctVal>1.788000215116</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Forgent Power Solutions, Inc.</name>
        <lei>529900VOEJB5GLOFQR48</lei>
        <title>Forgent Power Solutions, Inc., Class A</title>
        <cusip>34631F102</cusip>
        <identifiers>
          <isin value="US34631F1021"/>
        </identifiers>
        <balance>3248079.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>181437692.94000000</valUSD>
        <pctVal>3.306258471264</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Space Exploration Technologies Corp.</name>
        <lei>549300B9WLO96RQCXP87</lei>
        <title>Space Exploration Technologies Corp., Class A</title>
        <cusip>84615Q103</cusip>
        <identifiers>
          <isin value="US84615Q1031"/>
        </identifiers>
        <balance>267048.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>45627821.28000000</valUSD>
        <pctVal>0.831455516149</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>3M Co.</name>
        <lei>LUZQVYP4VS22CLWDAR65</lei>
        <title>3M Co.</title>
        <cusip>88579Y101</cusip>
        <identifiers>
          <isin value="US88579Y1010"/>
        </identifiers>
        <balance>860583.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>139336993.53000000</valUSD>
        <pctVal>2.539076129960</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Glaukos Corp.</name>
        <lei>5299004JA1LHE21M6T14</lei>
        <title>Glaukos Corp.</title>
        <cusip>377322102</cusip>
        <identifiers>
          <isin value="US3773221029"/>
        </identifiers>
        <balance>639308.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>89349686.08000000</valUSD>
        <pctVal>1.628179634121</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>UnitedHealth Group, Inc.</name>
        <lei>549300GHBMY8T5GXDE41</lei>
        <title>UnitedHealth Group, Inc.</title>
        <cusip>91324P102</cusip>
        <identifiers>
          <isin value="US91324P1021"/>
        </identifiers>
        <balance>535384.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>222521651.92000000</valUSD>
        <pctVal>4.054913203418</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>CenterPoint Energy, Inc.</name>
        <lei>21TPXMRRHFKOBHDC8J74</lei>
        <title>CenterPoint Energy, Inc.</title>
        <cusip>15189T107</cusip>
        <identifiers>
          <isin value="US15189T1079"/>
        </identifiers>
        <balance>2548349.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>112229289.96000000</valUSD>
        <pctVal>2.045104490922</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Chewy, Inc.</name>
        <lei>254900NPQSTG9I422879</lei>
        <title>Chewy, Inc., Class A</title>
        <cusip>16679L109</cusip>
        <identifiers>
          <isin value="US16679L1098"/>
        </identifiers>
        <balance>2600543.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>51100669.95000000</valUSD>
        <pctVal>0.931184806044</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Sotera Health Co.</name>
        <lei>N/A</lei>
        <title>Sotera Health Co.</title>
        <cusip>83601L102</cusip>
        <identifiers>
          <isin value="US83601L1026"/>
        </identifiers>
        <balance>5853280.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>103895720.00000000</valUSD>
        <pctVal>1.893245547890</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-07-30</ncom:dateSigned>
      <ncom:nameOfApplicant>Janus Investment Fund</ncom:nameOfApplicant>
      <ncom:signature>Jesper Nergaard</ncom:signature>
      <ncom:signerName>Jesper Nergaard</ncom:signerName>
      <ncom:title>VP, CFO, Treasurer, and Principal Accounting Officer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
