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        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Vanguard Value ETF</name>
        <lei>N/A</lei>
        <title>Vanguard Value ETF</title>
        <cusip>922908744</cusip>
        <identifiers>
          <isin value="US9229087443"/>
        </identifiers>
        <balance>35742.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>7789254.06000000</valUSD>
        <pctVal>3.603327431235</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Enterprise Fund</name>
        <lei>TJ2Y3QQ1QRI8KQ84FR29</lei>
        <title>Janus Henderson Enterprise Fund, Class N</title>
        <cusip>47103D405</cusip>
        <identifiers>
          <isin value="US47103D4051"/>
        </identifiers>
        <balance>112895.23210000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>17813738.67000000</valUSD>
        <pctVal>8.240677824607</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson High-Yield Fund</name>
        <lei>EL1Q72EQG65N1PYH1412</lei>
        <title>Janus Henderson High-Yield Fund, Class N</title>
        <cusip>47103D850</cusip>
        <identifiers>
          <isin value="US47103D8508"/>
        </identifiers>
        <balance>298731.68000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2234512.97000000</valUSD>
        <pctVal>1.033691007923</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Forty Fund</name>
        <lei>549300RDSCGTT8PWPM78</lei>
        <title>Janus Henderson Forty Fund, Class N</title>
        <cusip>47103D603</cusip>
        <identifiers>
          <isin value="US47103D6031"/>
        </identifiers>
        <balance>3231.35000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>205675.43000000</valUSD>
        <pctVal>0.095145942492</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson European Focus Fund</name>
        <lei>549300B6VJPM3NFA9J78</lei>
        <title>Janus Henderson European Focus Fund</title>
        <cusip>47103X591</cusip>
        <identifiers>
          <isin value="US47103X5914"/>
        </identifiers>
        <balance>183024.67860000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>11845357.20000000</valUSD>
        <pctVal>5.479690378920</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Cash Liquidity Fund LLC</name>
        <lei>N/A</lei>
        <title>Janus Henderson Cash Liquidity Fund LLC</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="Inhouse Asset ID" value="ABH9984U2"/>
        </identifiers>
        <balance>2147661.02190000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>2147659.82000000</valUSD>
        <pctVal>0.993512534417</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Developed World Bond Fund</name>
        <lei>JBEKRA4YPCM7Q2GORI65</lei>
        <title>Janus Henderson Developed World Bond Fund, Class N</title>
        <cusip>47103Y821</cusip>
        <identifiers>
          <isin value="US47103Y8214"/>
        </identifiers>
        <balance>2176029.55900000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>16994790.86000000</valUSD>
        <pctVal>7.861830622321</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Core MSCI Emerging Markets ETF</name>
        <lei>5493007C24Z3ZDJ0VD06</lei>
        <title>iShares Core MSCI Emerging Markets ETF</title>
        <cusip>46434G103</cusip>
        <identifiers>
          <isin value="US46434G1031"/>
        </identifiers>
        <balance>219281.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>18165238.04000000</valUSD>
        <pctVal>8.403282268142</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Growth and Income Fund</name>
        <lei>5Y0L1HFYUGXP9RNAEB69</lei>
        <title>Janus Henderson Growth and Income Fund, Class N</title>
        <cusip>47103D868</cusip>
        <identifiers>
          <isin value="US47103D8680"/>
        </identifiers>
        <balance>226884.06700000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>18039552.17000000</valUSD>
        <pctVal>8.345139686118</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Research Fund</name>
        <lei>TO5UP72VAT80SBB47C78</lei>
        <title>Janus Henderson Research Fund, Class N</title>
        <cusip>47103D827</cusip>
        <identifiers>
          <isin value="US47103D8276"/>
        </identifiers>
        <balance>181505.04200000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>17627769.68000000</valUSD>
        <pctVal>8.154648128071</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Adaptive Risk Managed US Equity Fund</name>
        <lei>5493006DGCHLVTOMO890</lei>
        <title>Janus Henderson Adaptive Risk Managed US Equity Fund, Class N</title>
        <cusip>47103E122</cusip>
        <identifiers>
          <isin value="US47103E1221"/>
        </identifiers>
        <balance>913692.63060000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>11731813.38000000</valUSD>
        <pctVal>5.427164738068</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Emerging Markets Debt Hard Currency ETF</name>
        <lei>529900QLTSH6GU0R4M68</lei>
        <title>Janus Henderson Emerging Markets Debt Hard Currency ETF</title>
        <cusip>47103U738</cusip>
        <identifiers>
          <isin value="US47103U7384"/>
        </identifiers>
        <balance>176414.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>9491955.27000000</valUSD>
        <pctVal>4.391001055684</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>iShares Russell 2000 Value ETF</name>
        <lei>549300WJAJCAZZLTQD63</lei>
        <title>iShares Russell 2000 Value ETF</title>
        <cusip>464287630</cusip>
        <identifiers>
          <isin value="US4642876308"/>
        </identifiers>
        <balance>40264.00000000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>8906396.80000000</valUSD>
        <pctVal>4.120120316490</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Janus Henderson Overseas Fund</name>
        <lei>SHOQ1W1OPKO6Q4WZ8151</lei>
        <title>Janus Henderson Overseas Fund, Class N</title>
        <cusip>47103D835</cusip>
        <identifiers>
          <isin value="US47103D8359"/>
        </identifiers>
        <balance>350803.03700000</balance>
        <units>NS</units>
        <curCd>USD</curCd>
        <valUSD>23058283.62000000</valUSD>
        <pctVal>10.66681677669</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetConditional assetCat="OTHER" desc="Investment company"/>
        <issuerCat>RF</issuerCat>
        <invCountry>US</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-07-30</ncom:dateSigned>
      <ncom:nameOfApplicant>Janus Investment Fund</ncom:nameOfApplicant>
      <ncom:signature>Jesper Nergaard</ncom:signature>
      <ncom:signerName>Jesper Nergaard</ncom:signerName>
      <ncom:title>VP, CFO, Treasurer, and Principal Accounting Officer</ncom:title>
    </signature>
  </formData>
</edgarSubmission>
