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        <name>Roche Holding AG</name>
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        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
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        <name>Japan Post Bank Co. Ltd.</name>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Janus Henderson Cash Collateral Fund LLC</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Recruit Holdings Co. Ltd.</name>
        <lei>353800R2LRSMVVC4I806</lei>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Daiichi Life Group, Inc.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Argenx SE</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Ascendis Pharma A/S</name>
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        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Spotify Technology SA</name>
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        <assetCat>EC</assetCat>
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        <invCountry>LU</invCountry>
        
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Taiwan Semiconductor Manufacturing Co. Ltd.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Industria de Diseno Textil SA</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Nippon Sanso Holdings Corp.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>LVMH Moet Hennessy Louis Vuitton SE</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Samsonite Group SA</name>
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        <isRestrictedSec>Y</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
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        <name>St James's Place plc</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Schneider Electric SE</name>
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        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
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        <name>Resona Holdings, Inc.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Canadian Dollar</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Danish Krone</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Freeport-McMoRan, Inc.</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Davide Campari-Milano NV</name>
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        <name>ASM International NV</name>
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        <fairValLevel>2</fairValLevel>
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        <fairValLevel>1</fairValLevel>
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        <name>Yen</name>
        <lei>N/A</lei>
        <title>Yen</title>
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        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
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      <invstOrSec>
        <name>Tencent Holdings Ltd.</name>
        <lei>254900N4SLUMW4XUYY11</lei>
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          <isin value="KYG875721634"/>
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        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
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        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Canadian Natural Resources Ltd.</name>
        <lei>549300T15RIMIKZC5Q38</lei>
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        <curCd>USD</curCd>
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        <assetCat>EC</assetCat>
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        <fairValLevel>1</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      <invstOrSec>
        <name>BAE Systems plc</name>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>ASML Holding NV</name>
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        <fairValLevel>2</fairValLevel>
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          <isCashCollateral>N</isCashCollateral>
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      <invstOrSec>
        <name>Rentokil Initial plc</name>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>SAP SE</name>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Hyundai Rotem Co. Ltd.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Janus Henderson Cash Liquidity Fund LLC</name>
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        <issuerCat>RF</issuerCat>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Erste Group Bank AG</name>
        <lei>PQOH26KWDF7CG10L6792</lei>
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        <invCountry>AT</invCountry>
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        <fairValLevel>2</fairValLevel>
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      <invstOrSec>
        <name>Contemporary Amperex Technology Co. Ltd.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Won</name>
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        <invCountry>KR</invCountry>
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        <fairValLevel>1</fairValLevel>
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        <name>Indian Rupee</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Banco Bilbao Vizcaya Argentaria SA</name>
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        <fairValLevel>2</fairValLevel>
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        <name>UniCredit SpA</name>
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        <fairValLevel>2</fairValLevel>
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        <name>AstraZeneca plc</name>
        <lei>PY6ZZQWO2IZFZC3IOL08</lei>
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        <invCountry>GB</invCountry>
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        <fairValLevel>2</fairValLevel>
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        <name>SK hynix, Inc.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>Safran SA</name>
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        <fairValLevel>2</fairValLevel>
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        <name>AIA Group Ltd.</name>
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        <fairValLevel>2</fairValLevel>
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        <name>TotalEnergies SE</name>
        <lei>529900S21EQ1BO4ESM68</lei>
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        <fairValLevel>2</fairValLevel>
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        <name>Gaztransport Et Technigaz SA</name>
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        <fairValLevel>2</fairValLevel>
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        <name>New Taiwan Dollar</name>
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        <fairValLevel>1</fairValLevel>
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        <name>Piraeus Bank SA</name>
        <lei>213800OYHR1MPQ5VJL60</lei>
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        <fairValLevel>2</fairValLevel>
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        <name>Heineken NV</name>
        <lei>724500K5PTPSST86UQ23</lei>
        <title>Heineken NV</title>
        <cusip>N/A</cusip>
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        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>NL</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Fujitsu Ltd.</name>
        <lei>3538000246DHJLRTUZ24</lei>
        <title>Fujitsu Ltd.</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="JP3818000006"/>
        </identifiers>
        <balance>2572900.00000000</balance>
        <units>NS</units>
        <currencyConditional curCd="JPY" exchangeRt="162.58000000"/>
        <valUSD>51350158.27000000</valUSD>
        <pctVal>1.119029256344</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>JP</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Deutsche Telekom AG</name>
        <lei>549300V9QSIG4WX4GJ96</lei>
        <title>Deutsche Telekom AG (Registered)</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="DE0005557508"/>
        </identifiers>
        <balance>3403381.00000000</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.87512033"/>
        <valUSD>92764353.40000000</valUSD>
        <pctVal>2.021532725462</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>DE</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Shin-Etsu Chemical Co. Ltd.</name>
        <lei>353800PX8Q64N86H5W41</lei>
        <title>Shin-Etsu Chemical Co. Ltd.</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="JP3371200001"/>
        </identifiers>
        <balance>994300.00000000</balance>
        <units>NS</units>
        <currencyConditional curCd="JPY" exchangeRt="162.58000000"/>
        <valUSD>43363002.28000000</valUSD>
        <pctVal>0.944972125287</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>JP</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>EURO</name>
        <lei>N/A</lei>
        <title>EURO</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="ISO Currency code" value="EUR"/>
        </identifiers>
        <balance>560015.69000000</balance>
        <units>OU</units>
        <descOthUnits>Currencies</descOthUnits>
        <currencyConditional curCd="EUR" exchangeRt="0.87512033"/>
        <valUSD>639929.93000000</valUSD>
        <pctVal>0.013945435375</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Foreign currencies" issuerCat="OTHER"/>
        <invCountry>XX</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Pound Sterling</name>
        <lei>N/A</lei>
        <title>Pound Sterling</title>
        <cusip>N/A</cusip>
        <identifiers>
          <other otherDesc="ISO Currency code" value="GBP"/>
        </identifiers>
        <balance>704134.92000000</balance>
        <units>OU</units>
        <descOthUnits>Currencies</descOthUnits>
        <currencyConditional curCd="GBP" exchangeRt="0.75374991"/>
        <valUSD>934175.80000000</valUSD>
        <pctVal>0.020357679235</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>STIV</assetCat>
        <issuerConditional desc="Foreign currencies" issuerCat="OTHER"/>
        <invCountry>GB</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>1</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>NatWest Group plc</name>
        <lei>2138005O9XJIJN4JPN90</lei>
        <title>NatWest Group plc</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="GB00BM8PJY71"/>
        </identifiers>
        <balance>6694823.99800000</balance>
        <units>NS</units>
        <currencyConditional curCd="GBP" exchangeRt="0.75374991"/>
        <valUSD>59019154.49000000</valUSD>
        <pctVal>1.286153008755</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>GB</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Ferrari NV</name>
        <lei>549300RIVY5EX8RCON76</lei>
        <title>Ferrari NV</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="NL0011585146"/>
        </identifiers>
        <balance>260582.00000000</balance>
        <units>NS</units>
        <currencyConditional curCd="EUR" exchangeRt="0.87512033"/>
        <valUSD>96581032.87000000</valUSD>
        <pctVal>2.104706295572</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>NL</invCountry>
        
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
      <invstOrSec>
        <name>Samsung Electronics Co. Ltd.</name>
        <lei>9884007ER46L6N7EI764</lei>
        <title>Samsung Electronics Co. Ltd.</title>
        <cusip>N/A</cusip>
        <identifiers>
          <isin value="KR7005930003"/>
        </identifiers>
        <balance>782318.00000000</balance>
        <units>NS</units>
        <currencyConditional curCd="KRW" exchangeRt="1548.90000000"/>
        <valUSD>171072217.12000000</valUSD>
        <pctVal>3.728027767673</pctVal>
        <payoffProfile>Long</payoffProfile>
        <assetCat>EC</assetCat>
        <issuerCat>CORP</issuerCat>
        <invCountry>KR</invCountry>
        <isRestrictedSec>N</isRestrictedSec>
        
        <fairValLevel>2</fairValLevel>
        <securityLending>
          <isCashCollateral>N</isCashCollateral>
          <isNonCashCollateral>N</isNonCashCollateral>
          <isLoanByFund>N</isLoanByFund>
        </securityLending>
      </invstOrSec>
    </invstOrSecs>
    <explntrNotes>
      <explntrNote note="Monthly returns presented in Item B.5(a) have been calculated without deducting any applicable sales loads or redemption fees." noteItem="B.5.a"/>
    </explntrNotes>
    <signature>
      <ncom:dateSigned>2026-07-30</ncom:dateSigned>
      <ncom:nameOfApplicant>Janus Investment Fund</ncom:nameOfApplicant>
      <ncom:signature>Jesper Nergaard</ncom:signature>
      <ncom:signerName>Jesper Nergaard</ncom:signerName>
      <ncom:title>VP, CFO, Treasurer, and Principal Accounting Officer</ncom:title>
    </signature>
  </formData>
  <documents>XXXX</documents>
</edgarSubmission>
