<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:01a0152c-0ec2-7e8a-8f06-87f3982fa711,g:6b1b3ea5-8794-445f-ae95-75d55dc2d20d-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.enterprisebank.com/role/DocumentandEntityInformationDocument" xlink:type="simple" xlink:href="efsc-20260820.xsd#DocumentandEntityInformationDocument"/>
  <link:definitionLink xlink:role="http://www.enterprisebank.com/role/DocumentandEntityInformationDocument" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_01a0152c-0ec2-7bca-b532-be8fda90573b" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_us-gaap_StatementTable_01a0152c-0ec2-7bca-b532-be8fda90573b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_01a0152c-0ec2-7c76-86e4-428a564cd663" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_01a0152c-0ec2-7bca-b532-be8fda90573b" xlink:to="loc_us-gaap_StatementClassOfStockAxis_01a0152c-0ec2-7c76-86e4-428a564cd663" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_01a0152c-0ec2-7c76-86e4-428a564cd663_default" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_01a0152c-0ec2-7c76-86e4-428a564cd663" xlink:to="loc_us-gaap_ClassOfStockDomain_01a0152c-0ec2-7c76-86e4-428a564cd663_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_01a0152c-0ec2-7717-8914-692348e98063" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_01a0152c-0ec2-7c76-86e4-428a564cd663" xlink:to="loc_us-gaap_ClassOfStockDomain_01a0152c-0ec2-7717-8914-692348e98063" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_01a0152c-0ec2-779e-9d66-d23e332b403d" xlink:href="https://xbrl.fasb.org/us-gaap/2024/elts/us-gaap-2024.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_01a0152c-0ec2-7717-8914-692348e98063" xlink:to="loc_us-gaap_CommonStockMember_01a0152c-0ec2-779e-9d66-d23e332b403d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_efsc_DepositarySharesMember_01a0152c-0ec2-7df1-a92b-64cb4dd81b14" xlink:href="efsc-20260820.xsd#efsc_DepositarySharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_01a0152c-0ec2-7717-8914-692348e98063" xlink:to="loc_efsc_DepositarySharesMember_01a0152c-0ec2-7df1-a92b-64cb4dd81b14" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_01a0152c-0ec2-792e-bf05-d8a7914d19d0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_DocumentType_01a0152c-0ec2-792e-bf05-d8a7914d19d0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentPeriodEndDate_01a0152c-0ec2-7b3a-9b82-6541a8edfd03" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_DocumentPeriodEndDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_DocumentPeriodEndDate_01a0152c-0ec2-7b3a-9b82-6541a8edfd03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_01a0152c-0ec2-781a-a409-07a32c3dddde" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityRegistrantName_01a0152c-0ec2-781a-a409-07a32c3dddde" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityIncorporationStateCountryCode_01a0152c-0ec2-7868-ab9e-f9fe6a8fce0b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityIncorporationStateCountryCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityIncorporationStateCountryCode_01a0152c-0ec2-7868-ab9e-f9fe6a8fce0b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityFileNumber_01a0152c-0ec2-7d50-9051-4ed6e1aa98af" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityFileNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityFileNumber_01a0152c-0ec2-7d50-9051-4ed6e1aa98af" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_01a0152c-0ec2-71cd-8448-0810899817dd" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityTaxIdentificationNumber_01a0152c-0ec2-71cd-8448-0810899817dd" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressAddressLine1_01a0152c-0ec2-7bf9-b908-d6354aa55638" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressAddressLine1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityAddressAddressLine1_01a0152c-0ec2-7bf9-b908-d6354aa55638" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressCityOrTown_01a0152c-0ec2-73b4-8f1c-794c9f792a4f" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressCityOrTown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityAddressCityOrTown_01a0152c-0ec2-73b4-8f1c-794c9f792a4f" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressStateOrProvince_01a0152c-0ec2-7375-a115-88df082e6a39" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressStateOrProvince"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityAddressStateOrProvince_01a0152c-0ec2-7375-a115-88df082e6a39" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityAddressPostalZipCode_01a0152c-0ec2-724b-9521-b77dfcf81623" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityAddressPostalZipCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityAddressPostalZipCode_01a0152c-0ec2-724b-9521-b77dfcf81623" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_CityAreaCode_01a0152c-0ec2-7bdb-8b0f-83a8f64bf6a6" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_CityAreaCode"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_CityAreaCode_01a0152c-0ec2-7bdb-8b0f-83a8f64bf6a6" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LocalPhoneNumber_01a0152c-0ec2-74a5-a1d6-5df0134b208d" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_LocalPhoneNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_LocalPhoneNumber_01a0152c-0ec2-74a5-a1d6-5df0134b208d" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_WrittenCommunications_01a0152c-0ec2-793d-aad7-cbbff29aefb0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_WrittenCommunications"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_WrittenCommunications_01a0152c-0ec2-793d-aad7-cbbff29aefb0" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SolicitingMaterial_01a0152c-0ec2-77db-a964-eafccd86e5fd" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SolicitingMaterial"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_SolicitingMaterial_01a0152c-0ec2-77db-a964-eafccd86e5fd" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementTenderOffer_01a0152c-0ec2-7c7c-828e-4719a01fa3c7" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementTenderOffer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_PreCommencementTenderOffer_01a0152c-0ec2-7c7c-828e-4719a01fa3c7" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_PreCommencementIssuerTenderOffer_01a0152c-0ec2-7185-b14d-aeb7ce02633b" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_PreCommencementIssuerTenderOffer"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_PreCommencementIssuerTenderOffer_01a0152c-0ec2-7185-b14d-aeb7ce02633b" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_Security12bTitle_01a0152c-0ec2-7fe2-8dc2-eceecc309b50" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_Security12bTitle"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_Security12bTitle_01a0152c-0ec2-7fe2-8dc2-eceecc309b50" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_TradingSymbol_01a0152c-0ec2-7f48-a9aa-d5f32f5078e2" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_TradingSymbol"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_TradingSymbol_01a0152c-0ec2-7f48-a9aa-d5f32f5078e2" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_SecurityExchangeName_01a0152c-0ec2-764a-ace8-2da5d9bd5cc0" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_SecurityExchangeName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_SecurityExchangeName_01a0152c-0ec2-764a-ace8-2da5d9bd5cc0" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityEmergingGrowthCompany_01a0152c-0ec2-79f3-a434-d8e6a4adb220" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityEmergingGrowthCompany"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityEmergingGrowthCompany_01a0152c-0ec2-79f3-a434-d8e6a4adb220" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_01a0152c-0ec2-71fb-89ac-948117b86432" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_EntityCentralIndexKey_01a0152c-0ec2-71fb-89ac-948117b86432" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_01a0152c-0ec2-799d-bb26-c1f98354d6d3" xlink:href="https://xbrl.sec.gov/dei/2024/dei-2024.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_01a0152c-0ec2-72f1-97ca-0bb9d9c12c55" xlink:to="loc_dei_AmendmentFlag_01a0152c-0ec2-799d-bb26-c1f98354d6d3" xlink:type="arc" order="21"/>
  </link:definitionLink>
</link:linkbase>
