<?xml version="1.0" encoding="US-ASCII"?>
<!-- Generated by Broadridge Transform (tm) - http://www.broadridge.com -->
<!-- Created: Mon Aug 10 14:14:20 UTC 2026 -->
<linkbase xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
  <roleRef xlink:type="simple" xlink:href="https://xbrl.sec.gov/oef/2026/oef-2026.xsd#ClassOnly" roleURI="http://xbrl.sec.gov/oef/role/ClassOnly"/>
  <roleRef xlink:type="simple" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-roles-2026.xsd#eedm1" roleURI="http://fasb.org/srt/role/srt-eedm/ExtensibleEnumerationLists"/>
  <roleRef xlink:type="simple" xlink:href="https://xbrl.sec.gov/oef/2026/oef-2026.xsd#LargestHoldingOnly" roleURI="http://xbrl.sec.gov/oef/role/LargestHoldingOnly"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member"/>
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all"/>
  <definitionLink xlink:role="http://xbrl.sec.gov/oef/role/ClassOnly" xlink:type="extended">
    <loc xlink:type="locator" xlink:label="oef_ShareClassDomain" xlink:href="https://xbrl.sec.gov/oef/2026/oef-2026.xsd#oef_ShareClassDomain"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_C000267166Member" xlink:href="ck0001771146-20260531.xsd#ck0001771146_C000267166Member"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="oef_ShareClassDomain" xlink:to="ck0001771146_C000267166Member" order="2" xbrldt:closed="true"/>
  </definitionLink>
  <definitionLink xlink:role="http://fasb.org/srt/role/srt-eedm/ExtensibleEnumerationLists" xlink:type="extended">
    <loc xlink:type="locator" xlink:label="us-gaap_InvestmentTypeCategorizationMember" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_CryptoCurrencyMember" xlink:href="ck0001771146-20260531.xsd#ck0001771146_CryptoCurrencyMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InvestmentTypeCategorizationMember" xlink:to="ck0001771146_CryptoCurrencyMember" order="2" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_MoneyMarketFundMember" xlink:href="ck0001771146-20260531.xsd#ck0001771146_MoneyMarketFundMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_InvestmentTypeCategorizationMember" xlink:to="ck0001771146_MoneyMarketFundMember" order="4" xbrldt:closed="true"/>
  </definitionLink>
  <definitionLink xlink:role="http://xbrl.sec.gov/oef/role/LargestHoldingOnly" xlink:type="extended">
    <loc xlink:type="locator" xlink:label="oef_LargestHoldingsDomain" xlink:href="https://xbrl.sec.gov/oef/2026/oef-2026.xsd#oef_LargestHoldingsDomain"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_GrayscaleBitcoinMiniTrustETFMember" xlink:href="ck0001771146-20260531.xsd#ck0001771146_GrayscaleBitcoinMiniTrustETFMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="oef_LargestHoldingsDomain" xlink:to="ck0001771146_GrayscaleBitcoinMiniTrustETFMember" order="2" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_iSharesStakedEthereumTrustETFMember" xlink:href="ck0001771146-20260531.xsd#ck0001771146_iSharesStakedEthereumTrustETFMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="oef_LargestHoldingsDomain" xlink:to="ck0001771146_iSharesStakedEthereumTrustETFMember" order="4" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_BitwiseSolanaStakingETFMember" xlink:href="ck0001771146-20260531.xsd#ck0001771146_BitwiseSolanaStakingETFMember"/>
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="oef_LargestHoldingsDomain" xlink:to="ck0001771146_BitwiseSolanaStakingETFMember" order="6" xbrldt:closed="true"/>
    <loc xlink:type="locator" xlink:label="ck0001771146_FirstAmericanGovernmentObligationsFundMember" xlink:href="ck0001771146-20260531.xsd#ck0001771146_FirstAmericanGovernmentObligationsFundMember"/>
<!-- [WMV7][fWCsI8Ohb9mOkwElNnh/mY0KL5RuWOL3poXWiV5B43M4Ic21vJYVCfawbDgsT0MAXT3r0vrmhU+Tc/gN6yj34rDIZguu9axVd4idhBcyz7KHX8dUmXIYu4mhDfvLYqNljW8I7QqyDif1mMXh9Tc3nfYVLGv1DUh1eRbC4ERNWGf3sLJVa2JjtOwUWVNy7YG9kCKVHRNcMjv1PduVoKcBkAmatBn+hz0tosFRBaEJMaDDTqQoLehzQSH9b/xCUmEzfOksi4La9yGEDrts8G6E7cSU+qeGyE/ROeRWEExUy44YrXSWshNtPEAserVPnvk0rEIJ8+VGUzPzu4iudZA59fBUiWecAFFlHq/UreQfJlXpvgUHVi8NAElUASJ8h1JYRPzlfzOOsw==] CSR-->
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="oef_LargestHoldingsDomain" xlink:to="ck0001771146_FirstAmericanGovernmentObligationsFundMember" order="8" xbrldt:closed="true"/>
  </definitionLink>
</linkbase>
