<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019e6850-cfdd-7290-ba98-cbff96447963,g:20d7760f-2405-4163-83f3-847f111fa9e6-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://establishmentlabs.com/role/CondensedConsolidatedStatementofShareholdersEquity" xlink:type="simple" xlink:href="esta-20260630.xsd#CondensedConsolidatedStatementofShareholdersEquity"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/CondensedConsolidatedStatementofShareholdersEquity" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019e6850-d155-7ddb-977c-4b44af7849b0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019e6850-d155-7327-a9da-c51e46f82834" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019e6850-d155-7ddb-977c-4b44af7849b0" xlink:to="loc_us-gaap_StatementTable_019e6850-d155-7327-a9da-c51e46f82834" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019e6850-d155-7715-bcab-c50a305b3794" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019e6850-d155-7327-a9da-c51e46f82834" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019e6850-d155-7715-bcab-c50a305b3794" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019e6850-d155-7715-bcab-c50a305b3794_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019e6850-d155-7715-bcab-c50a305b3794" xlink:to="loc_us-gaap_EquityComponentDomain_019e6850-d155-7715-bcab-c50a305b3794_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019e6850-d155-7715-bcab-c50a305b3794" xlink:to="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_019e6850-d155-7c5f-aa5b-7ddfb6df05be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:to="loc_us-gaap_CommonStockMember_019e6850-d155-7c5f-aa5b-7ddfb6df05be" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_019e6850-d155-7f94-b293-9f94f75cf635" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:to="loc_us-gaap_TreasuryStockCommonMember_019e6850-d155-7f94-b293-9f94f75cf635" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_019e6850-d155-70b9-86e7-c393b1d3fec5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_019e6850-d155-70b9-86e7-c393b1d3fec5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_019e6850-d155-79a5-9e3b-304f5a982976" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:to="loc_us-gaap_RetainedEarningsMember_019e6850-d155-79a5-9e3b-304f5a982976" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019e6850-d155-71e2-a670-73874d972288" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e6850-d155-70e2-86ee-f03cb811e5c8" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019e6850-d155-71e2-a670-73874d972288" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e6850-d155-7ddb-977c-4b44af7849b0" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019e6850-d155-78ea-8960-d66cffb64f54" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_019e6850-d155-78ea-8960-d66cffb64f54" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019e6850-d155-76de-ab88-99d5c3c7ad55" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockholdersEquity_019e6850-d155-76de-ab88-99d5c3c7ad55" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonShares_019e6850-d155-73ba-a647-317a592d905a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_TreasuryStockCommonShares_019e6850-d155-73ba-a647-317a592d905a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_019ed88b-beed-7192-a2fa-66e4e6ba6a24" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_019ed88b-beed-7192-a2fa-66e4e6ba6a24" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_019ed88b-beed-7a3a-b284-5f29df3abd9f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_019ed88b-beed-7a3a-b284-5f29df3abd9f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_019e6850-d155-7ca4-ba9c-1735e57f5e8a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_019e6850-d155-7ca4-ba9c-1735e57f5e8a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_019e6850-d155-73c9-b265-e37513cbe945" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueIssuedForServices"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueIssuedForServices_019e6850-d155-73c9-b265-e37513cbe945" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_019e6850-d155-76e5-a1e6-d1a379eba137" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_019e6850-d155-76e5-a1e6-d1a379eba137" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_019e6850-d155-7aff-9430-4f90918f9f58" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_019e6850-d155-7aff-9430-4f90918f9f58" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_019e6850-d155-7f0f-b58e-61ee9f39c0a8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesShareBasedCompensation_019e6850-d155-7f0f-b58e-61ee9f39c0a8" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_019e6850-d155-781b-a27e-64703943f681" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueShareBasedCompensation_019e6850-d155-781b-a27e-64703943f681" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation_019e6850-d155-7e53-9e7d-725d6ef517ae" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_SharesPaidForTaxWithholdingForShareBasedCompensation_019e6850-d155-7e53-9e7d-725d6ef517ae" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_019e6850-d155-7189-88d6-eb9647786aa8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_AdjustmentsRelatedToTaxWithholdingForShareBasedCompensation_019e6850-d155-7189-88d6-eb9647786aa8" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_019e6850-d155-79d9-bbd8-cbebe0176d5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_OtherComprehensiveIncomeForeignCurrencyTransactionAndTranslationAdjustmentNetOfTaxPortionAttributableToParent_019e6850-d155-79d9-bbd8-cbebe0176d5c" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e6850-d155-7339-981f-fb246a20fd70" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019e6850-d155-710a-8f60-dccc00fa4902" xlink:to="loc_us-gaap_NetIncomeLoss_019e6850-d155-7339-981f-fb246a20fd70" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019e6850-d155-7dbe-9c2d-0fa084cbca8e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019e6850-d155-7f95-86e4-3622191e3cbd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonShares_019e6850-d155-709f-87bb-cd1502657df9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonShares"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/SummaryofSignificantAccountingPoliciesScheduleofDisaggregationofRevenueDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#SummaryofSignificantAccountingPoliciesScheduleofDisaggregationofRevenueDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/SummaryofSignificantAccountingPoliciesScheduleofDisaggregationofRevenueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_019e6850-d155-7d99-becf-6b5b00e1e3a8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_019e6850-d155-77c6-97f5-3b7ae8234332" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_019e6850-d155-7d99-becf-6b5b00e1e3a8" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_019e6850-d155-77c6-97f5-3b7ae8234332" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_019e6850-d155-7c21-a021-1299dc40320b" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_019e6850-d155-77c6-97f5-3b7ae8234332" xlink:to="loc_srt_StatementGeographicalAxis_019e6850-d155-7c21-a021-1299dc40320b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_019e6850-d155-7c21-a021-1299dc40320b_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_019e6850-d155-7c21-a021-1299dc40320b" xlink:to="loc_srt_SegmentGeographicalDomain_019e6850-d155-7c21-a021-1299dc40320b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_019e6850-d155-72c2-9dcd-c9e0de4d26ba" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_019e6850-d155-7c21-a021-1299dc40320b" xlink:to="loc_srt_SegmentGeographicalDomain_019e6850-d155-72c2-9dcd-c9e0de4d26ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonUsMember_019e6850-d155-70ab-adba-73b4dadfa68c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NonUsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_019e6850-d155-72c2-9dcd-c9e0de4d26ba" xlink:to="loc_us-gaap_NonUsMember_019e6850-d155-70ab-adba-73b4dadfa68c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_019e6850-d155-74f4-81ad-a16359cf0bff" xlink:href="https://xbrl.sec.gov/country/2026/country-2026.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_019e6850-d155-72c2-9dcd-c9e0de4d26ba" xlink:to="loc_country_US_019e6850-d155-74f4-81ad-a16359cf0bff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e6850-d155-752d-8a96-1438423adf70" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_019e6850-d155-7d99-becf-6b5b00e1e3a8" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e6850-d155-752d-8a96-1438423adf70" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/FairValueMeasurementsScheduleofFairValueLiabilitiesMeasuredonRecurringBasisDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#FairValueMeasurementsScheduleofFairValueLiabilitiesMeasuredonRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/FairValueMeasurementsScheduleofFairValueLiabilitiesMeasuredonRecurringBasisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-74c0-9a02-7b5fd75ada3d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7c03-b33b-38481b833628" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-74c0-9a02-7b5fd75ada3d" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7c03-b33b-38481b833628" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e6850-d155-7714-8fd9-1a8d44f44afe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7c03-b33b-38481b833628" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e6850-d155-7714-8fd9-1a8d44f44afe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7714-8fd9-1a8d44f44afe_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e6850-d155-7714-8fd9-1a8d44f44afe" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7714-8fd9-1a8d44f44afe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7d1f-8521-d3babf76bb87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e6850-d155-7714-8fd9-1a8d44f44afe" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7d1f-8521-d3babf76bb87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019e6850-d155-79d0-a34e-17a6c23f25fa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7d1f-8521-d3babf76bb87" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019e6850-d155-79d0-a34e-17a6c23f25fa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019e6850-d155-70c3-9c07-4ea1113dc34f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7d1f-8521-d3babf76bb87" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019e6850-d155-70c3-9c07-4ea1113dc34f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019e6850-d155-71bf-9cbe-a9833988f2ef" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e6850-d155-7d1f-8521-d3babf76bb87" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019e6850-d155-71bf-9cbe-a9833988f2ef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_019e6850-d155-704e-9be2-337f1a8025c5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-74c0-9a02-7b5fd75ada3d" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_019e6850-d155-704e-9be2-337f1a8025c5" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-7b79-aab5-0d203f6f30aa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7f3a-a713-ca5e7bcff4c7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-7b79-aab5-0d203f6f30aa" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7f3a-a713-ca5e7bcff4c7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_019e6850-d155-7b66-a15b-7179fde2f6dc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7f3a-a713-ca5e7bcff4c7" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_019e6850-d155-7b66-a15b-7179fde2f6dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019e6850-d155-7b66-a15b-7179fde2f6dc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019e6850-d155-7b66-a15b-7179fde2f6dc" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019e6850-d155-7b66-a15b-7179fde2f6dc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019e6850-d155-7372-a471-0d73a18eac68" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019e6850-d155-7b66-a15b-7179fde2f6dc" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019e6850-d155-7372-a471-0d73a18eac68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputRevenueMultipleMember_019e6850-d155-7eb8-a08e-89fcc97ab4da" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputRevenueMultipleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019e6850-d155-7372-a471-0d73a18eac68" xlink:to="loc_us-gaap_MeasurementInputRevenueMultipleMember_019e6850-d155-7eb8-a08e-89fcc97ab4da" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_MeasurementInputForecastedRevenueBaseMember_019e6850-d155-7198-a4db-36be7aaf8522" xlink:href="esta-20260630.xsd#esta_MeasurementInputForecastedRevenueBaseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019e6850-d155-7372-a471-0d73a18eac68" xlink:to="loc_esta_MeasurementInputForecastedRevenueBaseMember_019e6850-d155-7198-a4db-36be7aaf8522" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019e6850-d155-74a5-a504-09e92e9434e6" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7f3a-a713-ca5e7bcff4c7" xlink:to="loc_srt_RangeAxis_019e6850-d155-74a5-a504-09e92e9434e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019e6850-d155-74a5-a504-09e92e9434e6_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019e6850-d155-74a5-a504-09e92e9434e6" xlink:to="loc_srt_RangeMember_019e6850-d155-74a5-a504-09e92e9434e6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019e6850-d155-70cd-939a-1bdeed13fb32" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019e6850-d155-74a5-a504-09e92e9434e6" xlink:to="loc_srt_RangeMember_019e6850-d155-70cd-939a-1bdeed13fb32" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019e6850-d155-7e37-b14f-e0df243537ce" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e6850-d155-70cd-939a-1bdeed13fb32" xlink:to="loc_srt_MinimumMember_019e6850-d155-7e37-b14f-e0df243537ce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019e6850-d155-781e-9fb7-05496142e740" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e6850-d155-70cd-939a-1bdeed13fb32" xlink:to="loc_srt_MaximumMember_019e6850-d155-781e-9fb7-05496142e740" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019facad-12ac-7d39-b707-a3bb68f6da69" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e6850-d155-7f3a-a713-ca5e7bcff4c7" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019facad-12ac-7d39-b707-a3bb68f6da69" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019facad-12ac-7d39-b707-a3bb68f6da69_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019facad-12ac-7d39-b707-a3bb68f6da69" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019facad-12ac-7d39-b707-a3bb68f6da69_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019facad-12ac-7ee7-8bfa-3d9a2d33054a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019facad-12ac-7d39-b707-a3bb68f6da69" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019facad-12ac-7ee7-8bfa-3d9a2d33054a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_MotivaBeneluxBVAndMotivaNLB.V.Member_019facad-12ac-7424-ae63-7161111b83e0" xlink:href="esta-20260630.xsd#esta_MotivaBeneluxBVAndMotivaNLB.V.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019facad-12ac-7ee7-8bfa-3d9a2d33054a" xlink:to="loc_esta_MotivaBeneluxBVAndMotivaNLB.V.Member_019facad-12ac-7424-ae63-7161111b83e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_019e6850-d155-7196-a6f3-791fd4d9a9c2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-7b79-aab5-0d203f6f30aa" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiabilityMeasurementInput_019e6850-d155-7196-a6f3-791fd4d9a9c2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_019facad-12ac-7ca4-885d-d56628c37464" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-7b79-aab5-0d203f6f30aa" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_019facad-12ac-7ca4-885d-d56628c37464" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_PaymentsToAcquireBusinessesGrossNonContingentContractualPayment_019facbd-8d4c-7e0b-a1b4-c80a9ebe1652" xlink:href="esta-20260630.xsd#esta_PaymentsToAcquireBusinessesGrossNonContingentContractualPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-7b79-aab5-0d203f6f30aa" xlink:to="loc_esta_PaymentsToAcquireBusinessesGrossNonContingentContractualPayment_019facbd-8d4c-7e0b-a1b4-c80a9ebe1652" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_019e6850-d155-7e57-a872-8857ea139c1e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e6850-d155-7b79-aab5-0d203f6f30aa" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_019e6850-d155-7e57-a872-8857ea139c1e" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/DebtNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019f8911-0d1a-75bf-aaa3-6914b26a5bfb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:to="loc_us-gaap_DebtInstrumentAxis_019f8911-0d1a-75bf-aaa3-6914b26a5bfb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019f8911-0d1a-75bf-aaa3-6914b26a5bfb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019f8911-0d1a-75bf-aaa3-6914b26a5bfb" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019f8911-0d1a-75bf-aaa3-6914b26a5bfb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019f8911-0d1a-7290-b688-1018ba6a7316" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019f8911-0d1a-75bf-aaa3-6914b26a5bfb" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019f8911-0d1a-7290-b688-1018ba6a7316" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_NewTermLoanMember_019f8911-0d1a-7ae5-99c6-e15c87de89a0" xlink:href="esta-20260630.xsd#esta_NewTermLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019f8911-0d1a-7290-b688-1018ba6a7316" xlink:to="loc_esta_NewTermLoanMember_019f8911-0d1a-7ae5-99c6-e15c87de89a0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_CreditAgreementTrancheETermLoanMember_019f8911-0d1a-7d69-9cc6-272f271e8139" xlink:href="esta-20260630.xsd#esta_CreditAgreementTrancheETermLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esta_NewTermLoanMember_019f8911-0d1a-7ae5-99c6-e15c87de89a0" xlink:to="loc_esta_CreditAgreementTrancheETermLoanMember_019f8911-0d1a-7d69-9cc6-272f271e8139" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_CreditAgreementTrancheFTermLoanMember_019f8911-0d1a-798a-a46b-aca8f2356e31" xlink:href="esta-20260630.xsd#esta_CreditAgreementTrancheFTermLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esta_NewTermLoanMember_019f8911-0d1a-7ae5-99c6-e15c87de89a0" xlink:to="loc_esta_CreditAgreementTrancheFTermLoanMember_019f8911-0d1a-798a-a46b-aca8f2356e31" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_OaktreeCreditAgreementMember_019fb617-2795-743a-a1f4-035bb5de0701" xlink:href="esta-20260630.xsd#esta_OaktreeCreditAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019f8911-0d1a-7290-b688-1018ba6a7316" xlink:to="loc_esta_OaktreeCreditAgreementMember_019fb617-2795-743a-a1f4-035bb5de0701" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019fa306-1326-7bf5-a0c4-6ef8b156dd7f" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:to="loc_srt_RangeAxis_019fa306-1326-7bf5-a0c4-6ef8b156dd7f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019fa306-1326-7bf5-a0c4-6ef8b156dd7f_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019fa306-1326-7bf5-a0c4-6ef8b156dd7f" xlink:to="loc_srt_RangeMember_019fa306-1326-7bf5-a0c4-6ef8b156dd7f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019fa306-1326-7520-95af-0369f2e3e204" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019fa306-1326-7bf5-a0c4-6ef8b156dd7f" xlink:to="loc_srt_RangeMember_019fa306-1326-7520-95af-0369f2e3e204" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019fa306-e36a-7293-b73f-a7aa7a07ae03" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019fa306-1326-7520-95af-0369f2e3e204" xlink:to="loc_srt_MinimumMember_019fa306-e36a-7293-b73f-a7aa7a07ae03" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019fa306-8817-7e11-9463-6ff0b3e37417" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019fa306-1326-7520-95af-0369f2e3e204" xlink:to="loc_srt_MaximumMember_019fa306-8817-7e11-9463-6ff0b3e37417" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019f8911-0d1a-7630-aae7-57f590eebc9c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019f8911-0d1a-7630-aae7-57f590eebc9c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f8911-0d1a-7630-aae7-57f590eebc9c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f8911-0d1a-7630-aae7-57f590eebc9c" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f8911-0d1a-7630-aae7-57f590eebc9c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f8911-0d1a-7d3b-8554-2bceac506db1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f8911-0d1a-7630-aae7-57f590eebc9c" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f8911-0d1a-7d3b-8554-2bceac506db1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_019f8911-0d1a-7373-81c8-ee33cae9f464" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019f8911-0d1a-7d3b-8554-2bceac506db1" xlink:to="loc_us-gaap_LineOfCreditMember_019f8911-0d1a-7373-81c8-ee33cae9f464" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_TriggeringEventAxis_019f8911-0d1a-7b2c-8fec-464afbeeda13" xlink:href="esta-20260630.xsd#esta_TriggeringEventAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:to="loc_esta_TriggeringEventAxis_019f8911-0d1a-7b2c-8fec-464afbeeda13" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_TriggeringEventDomain_019f8911-0d1a-7b2c-8fec-464afbeeda13_default" xlink:href="esta-20260630.xsd#esta_TriggeringEventDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_esta_TriggeringEventAxis_019f8911-0d1a-7b2c-8fec-464afbeeda13" xlink:to="loc_esta_TriggeringEventDomain_019f8911-0d1a-7b2c-8fec-464afbeeda13_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_TriggeringEventDomain_019f8911-0d1a-725d-aec6-87fa87b53950" xlink:href="esta-20260630.xsd#esta_TriggeringEventDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_esta_TriggeringEventAxis_019f8911-0d1a-7b2c-8fec-464afbeeda13" xlink:to="loc_esta_TriggeringEventDomain_019f8911-0d1a-725d-aec6-87fa87b53950" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_TriggeringEventElectionToPayUpToFiftyPercentOfInterestInKindMember_019f8911-0d1a-701b-81e8-aed73e59bdc0" xlink:href="esta-20260630.xsd#esta_TriggeringEventElectionToPayUpToFiftyPercentOfInterestInKindMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esta_TriggeringEventDomain_019f8911-0d1a-725d-aec6-87fa87b53950" xlink:to="loc_esta_TriggeringEventElectionToPayUpToFiftyPercentOfInterestInKindMember_019f8911-0d1a-701b-81e8-aed73e59bdc0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_TriggeringEventElectionToPayMoreThanFiftyPercentOfInterestInKindMember_019f8911-0d1a-7c13-a4ec-216a3ce87ca5" xlink:href="esta-20260630.xsd#esta_TriggeringEventElectionToPayMoreThanFiftyPercentOfInterestInKindMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esta_TriggeringEventDomain_019f8911-0d1a-725d-aec6-87fa87b53950" xlink:to="loc_esta_TriggeringEventElectionToPayMoreThanFiftyPercentOfInterestInKindMember_019f8911-0d1a-7c13-a4ec-216a3ce87ca5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_TriggeringEventAchievementOfGrossLeverageRatioLessThanFourMember_019f8911-0d1a-733e-9644-3fcc8116c914" xlink:href="esta-20260630.xsd#esta_TriggeringEventAchievementOfGrossLeverageRatioLessThanFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_esta_TriggeringEventDomain_019f8911-0d1a-725d-aec6-87fa87b53950" xlink:to="loc_esta_TriggeringEventAchievementOfGrossLeverageRatioLessThanFourMember_019f8911-0d1a-733e-9644-3fcc8116c914" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019f8911-6933-7647-b8e4-5022c808ac5f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019f8911-0d1a-7053-a714-b16fb297e828" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019f8911-6933-7647-b8e4-5022c808ac5f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019f8911-6933-7647-b8e4-5022c808ac5f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019f8911-6933-7647-b8e4-5022c808ac5f" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019f8911-6933-7647-b8e4-5022c808ac5f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019f8911-6933-7efd-9d46-f0e5b349464e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019f8911-6933-7647-b8e4-5022c808ac5f" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019f8911-6933-7efd-9d46-f0e5b349464e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodOneMember_019f8911-6933-7749-a665-36533fc9bd69" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019f8911-6933-7efd-9d46-f0e5b349464e" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodOneMember_019f8911-6933-7749-a665-36533fc9bd69" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodTwoMember_019f8911-6933-781c-acd7-e65e04145a3d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019f8911-6933-7efd-9d46-f0e5b349464e" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodTwoMember_019f8911-6933-781c-acd7-e65e04145a3d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_019f8911-0d1a-7ece-bb35-982f5d470143" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_019f8911-0d1a-7ece-bb35-982f5d470143" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentNumberOfTranches_019f893d-7cf1-7659-8ce3-a85f6fd45dc4" xlink:href="esta-20260630.xsd#esta_DebtInstrumentNumberOfTranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentNumberOfTranches_019f893d-7cf1-7659-8ce3-a85f6fd45dc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_019f8911-0d1a-7831-9790-01d5f124024f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_019f8911-0d1a-7831-9790-01d5f124024f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentOriginalIssueDiscountPercentage_019f8911-0d1a-7fd9-806f-98cae551fe74" xlink:href="esta-20260630.xsd#esta_DebtInstrumentOriginalIssueDiscountPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentOriginalIssueDiscountPercentage_019f8911-0d1a-7fd9-806f-98cae551fe74" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLinesOfCredit_019fba9a-3a4c-7204-8bea-fddca5311773" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RepaymentsOfLinesOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_RepaymentsOfLinesOfCredit_019fba9a-3a4c-7204-8bea-fddca5311773" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_019f8911-0d1a-78c2-a97c-aa46969022a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_019f8911-0d1a-78c2-a97c-aa46969022a9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateIncreaseDecrease_019f8911-0d1a-7515-9d4a-d52e6ca173a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_DebtInstrumentInterestRateIncreaseDecrease_019f8911-0d1a-7515-9d4a-d52e6ca173a9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentCovenantGrossLeverageRatioMaximum_019f8911-0d1a-7f1c-8223-c7415ad3e3c0" xlink:href="esta-20260630.xsd#esta_DebtInstrumentCovenantGrossLeverageRatioMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentCovenantGrossLeverageRatioMaximum_019f8911-0d1a-7f1c-8223-c7415ad3e3c0" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentExitFeePercentage_019f8911-0d1a-7a88-b3ae-205d97c6aa63" xlink:href="esta-20260630.xsd#esta_DebtInstrumentExitFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentExitFeePercentage_019f8911-0d1a-7a88-b3ae-205d97c6aa63" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentCovenantMinimumPrepaymentAmount_019f8911-0d1a-73fc-9cce-22b5e00d9e46" xlink:href="esta-20260630.xsd#esta_DebtInstrumentCovenantMinimumPrepaymentAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentCovenantMinimumPrepaymentAmount_019f8911-0d1a-73fc-9cce-22b5e00d9e46" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_019f8911-6933-7c82-9665-03302c1f1532" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_019f8911-6933-7c82-9665-03302c1f1532" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentYieldProtectionPremiumIncrementalAmountOfPrincipalPercentage_019f8911-6933-771f-9dd9-d33f30111bcc" xlink:href="esta-20260630.xsd#esta_DebtInstrumentYieldProtectionPremiumIncrementalAmountOfPrincipalPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentYieldProtectionPremiumIncrementalAmountOfPrincipalPercentage_019f8911-6933-771f-9dd9-d33f30111bcc" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentCovenantMinimumLiquidityRequirement_019f8911-6933-7e2f-9e76-05d9d766fa61" xlink:href="esta-20260630.xsd#esta_DebtInstrumentCovenantMinimumLiquidityRequirement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentCovenantMinimumLiquidityRequirement_019f8911-6933-7e2f-9e76-05d9d766fa61" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentCovenantMinimumGrossSalesRequirementConsecutivePeriod_019f8911-6933-7453-b605-3b3d9308e4db" xlink:href="esta-20260630.xsd#esta_DebtInstrumentCovenantMinimumGrossSalesRequirementConsecutivePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentCovenantMinimumGrossSalesRequirementConsecutivePeriod_019f8911-6933-7453-b605-3b3d9308e4db" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentCashInterestPaymentPercentage_019fba89-e2fd-7110-a79b-5d5a06ddcfe7" xlink:href="esta-20260630.xsd#esta_DebtInstrumentCashInterestPaymentPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentCashInterestPaymentPercentage_019fba89-e2fd-7110-a79b-5d5a06ddcfe7" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DebtInstrumentStepUpCashInterestRateCharge_019fba89-112e-7f77-b7bc-1d765995f0f2" xlink:href="esta-20260630.xsd#esta_DebtInstrumentStepUpCashInterestRateCharge"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_DebtInstrumentStepUpCashInterestRateCharge_019fba89-112e-7f77-b7bc-1d765995f0f2" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebt_019f8911-8eb2-71cc-b6d2-36260261f6a8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_InterestExpenseDebt_019f8911-8eb2-71cc-b6d2-36260261f6a8" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_019f8911-8eb2-7723-8058-575bdfdc1ec4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_019f8911-8eb2-7723-8058-575bdfdc1ec4" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrent_019fb696-0103-7288-921f-0627c375fee9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_us-gaap_InterestPayableCurrent_019fb696-0103-7288-921f-0627c375fee9" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_AccruedDebtExtinguishmentExitFee_019f8911-8eb2-727e-baa1-167ec935e222" xlink:href="esta-20260630.xsd#esta_AccruedDebtExtinguishmentExitFee"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019f8911-0d1a-75b7-908e-85fa24b53268" xlink:to="loc_esta_AccruedDebtExtinguishmentExitFee_019f8911-8eb2-727e-baa1-167ec935e222" xlink:type="arc" order="19"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/DebtScheduleofDebtDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#DebtScheduleofDebtDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/DebtScheduleofDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_019e6850-d155-77c2-8b6b-252cfcabdddb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_019e6850-d155-7a78-ad2e-84f1612fba3a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019e6850-d155-77c2-8b6b-252cfcabdddb" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_019e6850-d155-7a78-ad2e-84f1612fba3a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019e6850-d155-7dfe-a160-6a7d9d166922" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019e6850-d155-7a78-ad2e-84f1612fba3a" xlink:to="loc_us-gaap_DebtInstrumentAxis_019e6850-d155-7dfe-a160-6a7d9d166922" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019e6850-d155-7dfe-a160-6a7d9d166922_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019e6850-d155-7dfe-a160-6a7d9d166922" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019e6850-d155-7dfe-a160-6a7d9d166922_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019e6850-d155-728e-a934-2fa9e46ddd88" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019e6850-d155-7dfe-a160-6a7d9d166922" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019e6850-d155-728e-a934-2fa9e46ddd88" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_NewTermLoanMember_019ed989-fa69-7c31-8a0d-44b42ee05286" xlink:href="esta-20260630.xsd#esta_NewTermLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019e6850-d155-728e-a934-2fa9e46ddd88" xlink:to="loc_esta_NewTermLoanMember_019ed989-fa69-7c31-8a0d-44b42ee05286" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019e6850-d155-7138-878b-146aae66554c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_019e6850-d155-7a78-ad2e-84f1612fba3a" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019e6850-d155-7138-878b-146aae66554c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019e6850-d155-7138-878b-146aae66554c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019e6850-d155-7138-878b-146aae66554c" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019e6850-d155-7138-878b-146aae66554c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019e6850-d155-7fca-ad12-e8c9b1cc431a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019e6850-d155-7138-878b-146aae66554c" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019e6850-d155-7fca-ad12-e8c9b1cc431a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_019e6850-d155-725e-8263-900bc01898a4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019e6850-d155-7fca-ad12-e8c9b1cc431a" xlink:to="loc_us-gaap_LineOfCreditMember_019e6850-d155-725e-8263-900bc01898a4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_019e6850-d155-747f-aea0-b57d745ab6c8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019e6850-d155-77c2-8b6b-252cfcabdddb" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_019e6850-d155-747f-aea0-b57d745ab6c8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumNet_019e6850-d155-7f8f-9efa-06ef467fc4aa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentUnamortizedDiscountPremiumNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019e6850-d155-77c2-8b6b-252cfcabdddb" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumNet_019e6850-d155-7f8f-9efa-06ef467fc4aa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_019e6850-d155-7c2b-88c9-6e1b95e56c10" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_019e6850-d155-77c2-8b6b-252cfcabdddb" xlink:to="loc_us-gaap_LongTermDebt_019e6850-d155-7c2b-88c9-6e1b95e56c10" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/NetLossPerShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#NetLossPerShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/NetLossPerShareScheduleofAntidilutiveSecuritiesExcludedfromComputationofEarningsPerShareDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_019e6850-d215-7b39-aa50-3459fc09b717" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_019e6850-d215-7520-aa87-c5ebf56cfb00" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_019e6850-d215-7b39-aa50-3459fc09b717" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_019e6850-d215-7520-aa87-c5ebf56cfb00" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019e6850-d215-7947-8e38-42b454a67777" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_019e6850-d215-7520-aa87-c5ebf56cfb00" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019e6850-d215-7947-8e38-42b454a67777" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019e6850-d215-7947-8e38-42b454a67777_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019e6850-d215-7947-8e38-42b454a67777" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019e6850-d215-7947-8e38-42b454a67777_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019e6850-d215-7e75-b895-e2d2ccb10553" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019e6850-d215-7947-8e38-42b454a67777" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019e6850-d215-7e75-b895-e2d2ccb10553" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_EmployeeAndNonemployeeStockOptionMember_019e6850-d215-709a-a6d7-e01dc9ddcc05" xlink:href="esta-20260630.xsd#esta_EmployeeAndNonemployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019e6850-d215-7e75-b895-e2d2ccb10553" xlink:to="loc_esta_EmployeeAndNonemployeeStockOptionMember_019e6850-d215-709a-a6d7-e01dc9ddcc05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019e6850-d215-76d1-9588-411dde25c4be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019e6850-d215-7e75-b895-e2d2ccb10553" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019e6850-d215-76d1-9588-411dde25c4be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019e6850-d215-7e1e-9786-9c201882974c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_019e6850-d215-7b39-aa50-3459fc09b717" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019e6850-d215-7e1e-9786-9c201882974c" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://establishmentlabs.com/role/RelatedPartyTransactionsDetails" xlink:type="simple" xlink:href="esta-20260630.xsd#RelatedPartyTransactionsDetails"/>
  <link:definitionLink xlink:role="http://establishmentlabs.com/role/RelatedPartyTransactionsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_019e6850-d215-75a1-b45d-2d4bd56b32f9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_019e6850-d215-75a1-b45d-2d4bd56b32f9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_019e6850-d215-7449-9884-9a64f5667eeb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_019e6850-d215-75a1-b45d-2d4bd56b32f9" xlink:to="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_019e6850-d215-7449-9884-9a64f5667eeb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_019e6850-d215-7449-9884-9a64f5667eeb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_019e6850-d215-7449-9884-9a64f5667eeb" xlink:to="loc_us-gaap_RelatedPartyDomain_019e6850-d215-7449-9884-9a64f5667eeb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyDomain_019e6850-d215-7860-9a40-25e933ec18d7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_019e6850-d215-7449-9884-9a64f5667eeb" xlink:to="loc_us-gaap_RelatedPartyDomain_019e6850-d215-7860-9a40-25e933ec18d7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImmediateFamilyMemberOfManagementOrPrincipalOwnerMember_019e6850-d215-7e42-889f-949f77151239" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ImmediateFamilyMemberOfManagementOrPrincipalOwnerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_019e6850-d215-7860-9a40-25e933ec18d7" xlink:to="loc_us-gaap_ImmediateFamilyMemberOfManagementOrPrincipalOwnerMember_019e6850-d215-7e42-889f-949f77151239" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyMember_019e6850-d215-7d35-8de9-6492b8308376" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyDomain_019e6850-d215-7860-9a40-25e933ec18d7" xlink:to="loc_us-gaap_RelatedPartyMember_019e6850-d215-7d35-8de9-6492b8308376" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionAxis_019e6850-d215-7955-80e9-1dfd2c57554f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRelatedPartyTransactionsByRelatedPartyTable_019e6850-d215-75a1-b45d-2d4bd56b32f9" xlink:to="loc_us-gaap_RelatedPartyTransactionAxis_019e6850-d215-7955-80e9-1dfd2c57554f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7955-80e9-1dfd2c57554f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_019e6850-d215-7955-80e9-1dfd2c57554f" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7955-80e9-1dfd2c57554f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7a1a-a3e2-3a855d389390" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RelatedPartyTransactionAxis_019e6850-d215-7955-80e9-1dfd2c57554f" xlink:to="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7a1a-a3e2-3a855d389390" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_HerramientasMedicasSAMember_019e6850-d215-7e49-b675-b027f946af06" xlink:href="esta-20260630.xsd#esta_HerramientasMedicasSAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7a1a-a3e2-3a855d389390" xlink:to="loc_esta_HerramientasMedicasSAMember_019e6850-d215-7e49-b675-b027f946af06" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_DrChaconQuirosMember_019f8948-7d31-7bb1-8635-f5aa94af33c6" xlink:href="esta-20260630.xsd#esta_DrChaconQuirosMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7a1a-a3e2-3a855d389390" xlink:to="loc_esta_DrChaconQuirosMember_019f8948-7d31-7bb1-8635-f5aa94af33c6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_NicholasLewinBoDMember_019e6850-d215-7654-be15-b6d507fe1b59" xlink:href="esta-20260630.xsd#esta_NicholasLewinBoDMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionDomain_019e6850-d215-7a1a-a3e2-3a855d389390" xlink:to="loc_esta_NicholasLewinBoDMember_019e6850-d215-7654-be15-b6d507fe1b59" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e6850-d215-7885-a9df-9fc4d0eef572" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e6850-d215-7885-a9df-9fc4d0eef572" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_019e6850-d215-771b-8cb0-6db424174cdb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_us-gaap_AccountsPayableCurrent_019e6850-d215-771b-8cb0-6db424174cdb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_RelatedPartyTransactionFeeRatePercentageOfAnnualNetSalesOfQualifyingProducts_019f894b-60ec-75cc-89ff-55e9ef1bc39f" xlink:href="esta-20260630.xsd#esta_RelatedPartyTransactionFeeRatePercentageOfAnnualNetSalesOfQualifyingProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_esta_RelatedPartyTransactionFeeRatePercentageOfAnnualNetSalesOfQualifyingProducts_019f894b-60ec-75cc-89ff-55e9ef1bc39f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RoyaltyExpense_019f8948-7d31-72f0-bdd5-9bda8cfb93c6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RoyaltyExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_us-gaap_RoyaltyExpense_019f8948-7d31-72f0-bdd5-9bda8cfb93c6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_019e6850-d215-741a-946e-5419ed11d477" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_019e6850-d215-741a-946e-5419ed11d477" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantedInPeriodFairValue_019e6850-d215-78eb-88cc-add592c92780" xlink:href="esta-20260630.xsd#esta_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantedInPeriodFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RelatedPartyTransactionLineItems_019e6850-d215-7d52-af4a-0c548cf3e37c" xlink:to="loc_esta_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantedInPeriodFairValue_019e6850-d215-78eb-88cc-add592c92780" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2026/ecd-2026.xsd#IndividualsOnly"/>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_ecd_AllIndividualsMember" xlink:href="https://xbrl.sec.gov/ecd/2026/ecd-2026.xsd#ecd_AllIndividualsMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_EdwardJ.SchutterMember_019fa1dd-5726-7c4f-9bd0-12b17eb5096f" xlink:href="esta-20260630.xsd#esta_EdwardJ.SchutterMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ecd_AllIndividualsMember" xlink:to="loc_esta_EdwardJ.SchutterMember_019fa1dd-5726-7c4f-9bd0-12b17eb5096f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_esta_BryanSlotkinMember_019fa1e7-028d-7e49-b29d-5bf0417bd194" xlink:href="esta-20260630.xsd#esta_BryanSlotkinMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_ecd_AllIndividualsMember" xlink:to="loc_esta_BryanSlotkinMember_019fa1e7-028d-7e49-b29d-5bf0417bd194" xlink:type="arc" order="3"/>
  </link:definitionLink>
</link:linkbase>
