<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019e4114-4eb9-745d-b7e0-e0a7d38f9919,g:d52baf7e-edd4-4478-acf0-9a36f3803232-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.midpennbank.com/role/CONSOLIDATEDSTATEMENTSOFINCOMEUNAUDITED" xlink:type="simple" xlink:href="mpb-20260630.xsd#CONSOLIDATEDSTATEMENTSOFINCOMEUNAUDITED"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/CONSOLIDATEDSTATEMENTSOFINCOMEUNAUDITED" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019e4114-5597-7ddf-b8de-96dba197f120" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_StatementTable_019e4114-5597-7ddf-b8de-96dba197f120" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_019e4114-5597-7a6e-9875-dac60276ef45" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019e4114-5597-7ddf-b8de-96dba197f120" xlink:to="loc_srt_ProductOrServiceAxis_019e4114-5597-7a6e-9875-dac60276ef45" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019e4114-5597-7a6e-9875-dac60276ef45_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_019e4114-5597-7a6e-9875-dac60276ef45" xlink:to="loc_srt_ProductsAndServicesDomain_019e4114-5597-7a6e-9875-dac60276ef45_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_019e4114-5597-7a6e-9875-dac60276ef45" xlink:to="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiduciaryAndTrustMember_019e4114-5597-70ff-acc9-7e9d466d8049" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiduciaryAndTrustMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:to="loc_us-gaap_FiduciaryAndTrustMember_019e4114-5597-70ff-acc9-7e9d466d8049" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebitCardMember_019e4114-5597-7672-96ca-506d5cec5d7e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebitCardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:to="loc_us-gaap_DebitCardMember_019e4114-5597-7672-96ca-506d5cec5d7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositAccountMember_019e4114-5597-7309-8d6a-900753e75dc4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepositAccountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:to="loc_us-gaap_DepositAccountMember_019e4114-5597-7309-8d6a-900753e75dc4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBankingMember_019e4114-5597-7b4f-b69b-1539df7d8ae1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBankingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:to="loc_us-gaap_MortgageBankingMember_019e4114-5597-7b4f-b69b-1539df7d8ae1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_MortgageHedgingMember_019e4114-5597-7d09-8db1-4fc9e52971d5" xlink:href="mpb-20260630.xsd#mpb_MortgageHedgingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019e4114-5597-7553-b319-380795035be4" xlink:to="loc_mpb_MortgageHedgingMember_019e4114-5597-7d09-8db1-4fc9e52971d5" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestAndDividendIncomeOperatingAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndFeeIncomeLoansAndLeases_019e4114-5597-778f-81f1-7dc9109e4bfd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestAndFeeIncomeLoansAndLeases"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:to="loc_us-gaap_InterestAndFeeIncomeLoansAndLeases_019e4114-5597-778f-81f1-7dc9109e4bfd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeSecuritiesByClassificationAbstract_019e4114-5597-7f06-927e-116b66c4f288" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestAndDividendIncomeSecuritiesByClassificationAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:to="loc_us-gaap_InterestAndDividendIncomeSecuritiesByClassificationAbstract_019e4114-5597-7f06-927e-116b66c4f288" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeSecuritiesUSTreasuryAndOtherUSGovernment_019e4114-5597-72ec-8978-56abeae760a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeSecuritiesUSTreasuryAndOtherUSGovernment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeSecuritiesByClassificationAbstract_019e4114-5597-7f06-927e-116b66c4f288" xlink:to="loc_us-gaap_InterestIncomeSecuritiesUSTreasuryAndOtherUSGovernment_019e4114-5597-72ec-8978-56abeae760a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeSecuritiesStateAndMunicipal_019e4114-5597-70a8-977a-3b85cac01baf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeSecuritiesStateAndMunicipal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeSecuritiesByClassificationAbstract_019e4114-5597-7f06-927e-116b66c4f288" xlink:to="loc_us-gaap_InterestIncomeSecuritiesStateAndMunicipal_019e4114-5597-70a8-977a-3b85cac01baf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeAndFeesBankersAcceptancesCertificatesOfDepositAndCommercialPaper_019e4114-5597-7c89-b7b9-40e86879df9e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeAndFeesBankersAcceptancesCertificatesOfDepositAndCommercialPaper"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:to="loc_us-gaap_InterestIncomeAndFeesBankersAcceptancesCertificatesOfDepositAndCommercialPaper_019e4114-5597-7c89-b7b9-40e86879df9e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeFederalFundsSold_019e4114-5597-72b8-b9c1-62441f9fb2cb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeFederalFundsSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:to="loc_us-gaap_InterestIncomeFederalFundsSold_019e4114-5597-72b8-b9c1-62441f9fb2cb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndDividendIncomeOperating_019e4114-5597-7216-bd27-33d962cbd1d8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestAndDividendIncomeOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestAndDividendIncomeOperatingAbstract_019e4114-5597-74bb-8076-6c51e3560197" xlink:to="loc_us-gaap_InterestAndDividendIncomeOperating_019e4114-5597-7216-bd27-33d962cbd1d8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseAbstract_019e4114-5597-78ba-aa66-2d2f60e24646" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_InterestExpenseAbstract_019e4114-5597-78ba-aa66-2d2f60e24646" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDomesticDeposits_019e4114-5597-7d12-a6d0-93c5300262e2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseDomesticDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_019e4114-5597-78ba-aa66-2d2f60e24646" xlink:to="loc_us-gaap_InterestExpenseDomesticDeposits_019e4114-5597-7d12-a6d0-93c5300262e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseShortTermBorrowings_019e4114-5597-7e49-b824-a7dd6738564b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_019e4114-5597-78ba-aa66-2d2f60e24646" xlink:to="loc_us-gaap_InterestExpenseShortTermBorrowings_019e4114-5597-7e49-b824-a7dd6738564b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_InterestOnLongTermAndSubordinatedDebt_019e4114-5597-7ccb-8a3d-b22e84a9eee3" xlink:href="mpb-20260630.xsd#mpb_InterestOnLongTermAndSubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_019e4114-5597-78ba-aa66-2d2f60e24646" xlink:to="loc_mpb_InterestOnLongTermAndSubordinatedDebt_019e4114-5597-7ccb-8a3d-b22e84a9eee3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseOperating_019e4114-5597-7f5e-9dc9-ef2ae06e07de" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseOperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InterestExpenseAbstract_019e4114-5597-78ba-aa66-2d2f60e24646" xlink:to="loc_us-gaap_InterestExpenseOperating_019e4114-5597-7f5e-9dc9-ef2ae06e07de" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseNet_019e4114-5597-78f0-a6f9-078d22cf0278" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeExpenseNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_InterestIncomeExpenseNet_019e4114-5597-78f0-a6f9-078d22cf0278" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLoanLossesExpensed_019e4114-5597-70a6-9e99-aa8954b306c3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProvisionForLoanLossesExpensed"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_ProvisionForLoanLossesExpensed_019e4114-5597-70a6-9e99-aa8954b306c3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForOtherCreditLosses_019e4114-5597-7547-abb8-87717e3b74fe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProvisionForOtherCreditLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_ProvisionForOtherCreditLosses_019e4114-5597-7547-abb8-87717e3b74fe" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForLoanLeaseAndOtherLosses_019e4114-5597-7ecd-b2f0-ebee13fc7d28" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProvisionForLoanLeaseAndOtherLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_ProvisionForLoanLeaseAndOtherLosses_019e4114-5597-7ecd-b2f0-ebee13fc7d28" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseAfterProvisionForLoanLoss_019e4114-5597-777f-bb80-ee4d7d0f4d5e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeExpenseAfterProvisionForLoanLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_InterestIncomeExpenseAfterProvisionForLoanLoss_019e4114-5597-777f-bb80-ee4d7d0f4d5e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestIncomeAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e4114-5597-7004-9843-aed57585776d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e4114-5597-7004-9843-aed57585776d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnSalesOfLoansNet_019e4114-5597-7b90-8171-7cac9498426b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GainLossOnSalesOfLoansNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:to="loc_us-gaap_GainLossOnSalesOfLoansNet_019e4114-5597-7b90-8171-7cac9498426b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BankOwnedLifeInsuranceIncome_019e4114-5597-73dc-9f38-9cc24bbfdff2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BankOwnedLifeInsuranceIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:to="loc_us-gaap_BankOwnedLifeInsuranceIncome_019e4114-5597-73dc-9f38-9cc24bbfdff2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncomeOtherOperatingIncome_019e4114-5597-7e3c-a2ad-c49e48743a99" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestIncomeOtherOperatingIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:to="loc_us-gaap_NoninterestIncomeOtherOperatingIncome_019e4114-5597-7e3c-a2ad-c49e48743a99" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncome_019e4114-5597-7d90-a06d-f2a9591e8926" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestIncomeAbstract_019e4114-5597-7a9c-91cd-494c5683d660" xlink:to="loc_us-gaap_NoninterestIncome_019e4114-5597-7d90-a06d-f2a9591e8926" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LaborAndRelatedExpense_019e4114-5597-7032-ac77-aa5af2fbb352" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LaborAndRelatedExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_LaborAndRelatedExpense_019e4114-5597-7032-ac77-aa5af2fbb352" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InformationTechnologyAndDataProcessing_019e4114-5597-79c6-b75d-4a6b4bfced19" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InformationTechnologyAndDataProcessing"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_InformationTechnologyAndDataProcessing_019e4114-5597-79c6-b75d-4a6b4bfced19" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OccupancyNet_019e4114-5597-7e36-b1b9-e63f2930ca4e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OccupancyNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_OccupancyNet_019e4114-5597-7e36-b1b9-e63f2930ca4e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentExpense_019e4114-5597-71ba-87ce-41dae1316cf3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquipmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_EquipmentExpense_019e4114-5597-71ba-87ce-41dae1316cf3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_SharesTaxExpense_019e4114-5597-730b-9370-4067a76da8b8" xlink:href="mpb-20260630.xsd#mpb_SharesTaxExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_mpb_SharesTaxExpense_019e4114-5597-730b-9370-4067a76da8b8" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfessionalFees_019e4114-5597-7ca7-b3d7-069543f0a954" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProfessionalFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_ProfessionalFees_019e4114-5597-7ca7-b3d7-069543f0a954" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CardProcessingFees_019e4114-5597-7502-8a7e-76962d843fdd" xlink:href="mpb-20260630.xsd#mpb_CardProcessingFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_mpb_CardProcessingFees_019e4114-5597-7502-8a7e-76962d843fdd" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_019e4114-5597-79a1-875e-c1300dd7bda5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_019e4114-5597-79a1-875e-c1300dd7bda5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FederalDepositInsuranceCorporationAssessmentExpenseBenefit_019e4114-5597-764d-8e4e-ad48cd9bc9d7" xlink:href="mpb-20260630.xsd#mpb_FederalDepositInsuranceCorporationAssessmentExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_mpb_FederalDepositInsuranceCorporationAssessmentExpenseBenefit_019e4114-5597-764d-8e4e-ad48cd9bc9d7" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_GainLossOnSaleOrWriteDownOfForeclosedAssets_019e4114-5597-7f42-acd3-db7f1b474a4a" xlink:href="mpb-20260630.xsd#mpb_GainLossOnSaleOrWriteDownOfForeclosedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_mpb_GainLossOnSaleOrWriteDownOfForeclosedAssets_019e4114-5597-7f42-acd3-db7f1b474a4a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_019e4114-5597-7570-b516-afa1dee968c1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationAcquisitionRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_019e4114-5597-7570-b516-afa1dee968c1" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoninterestExpense_019e4114-5597-7510-bca6-5d13983b4bb4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherNoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_OtherNoninterestExpense_019e4114-5597-7510-bca6-5d13983b4bb4" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestExpense_019e4114-5597-7899-ac1e-103e5c7885e2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_NoninterestExpenseAbstract_019e4114-5597-7d87-adb3-a4fe8f6eb826" xlink:to="loc_us-gaap_NoninterestExpense_019e4114-5597-7899-ac1e-103e5c7885e2" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e4114-5597-7cb0-bb22-6b39f5d795ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e4114-5597-7cb0-bb22-6b39f5d795ec" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019e4114-5597-7e94-a2fb-b4e22a6c97ca" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019e4114-5597-7e94-a2fb-b4e22a6c97ca" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e4114-5597-750d-8dd1-4e50006894f7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_NetIncomeLoss_019e4114-5597-750d-8dd1-4e50006894f7" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareAbstract_019e4114-5597-7444-9de5-85b6c748711e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-74a7-9c64-c465667bb27f" xlink:to="loc_us-gaap_EarningsPerShareAbstract_019e4114-5597-7444-9de5-85b6c748711e" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_019e4114-5597-71ba-9b7a-f0d10fb3ad08" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_019e4114-5597-7444-9de5-85b6c748711e" xlink:to="loc_us-gaap_EarningsPerShareBasic_019e4114-5597-71ba-9b7a-f0d10fb3ad08" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_019e4114-5597-76bb-b6dd-e63f08a5ba2a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_019e4114-5597-7444-9de5-85b6c748711e" xlink:to="loc_us-gaap_EarningsPerShareDiluted_019e4114-5597-76bb-b6dd-e63f08a5ba2a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019e4114-5597-727e-9fea-1f2d69b558d5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_019e4114-5597-7444-9de5-85b6c748711e" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019e4114-5597-727e-9fea-1f2d69b558d5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019e4114-5597-7fc6-9b00-32fa88ee83c4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EarningsPerShareAbstract_019e4114-5597-7444-9de5-85b6c748711e" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019e4114-5597-7fc6-9b00-32fa88ee83c4" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/CONSOLIDATEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITYUNAUDITED" xlink:type="simple" xlink:href="mpb-20260630.xsd#CONSOLIDATEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITYUNAUDITED"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/CONSOLIDATEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITYUNAUDITED" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019e4114-5597-714d-8391-bffefb46fb37" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StatementTable_019e4114-5597-714d-8391-bffefb46fb37" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019e4114-5597-7a99-addc-a5e2555978b7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019e4114-5597-714d-8391-bffefb46fb37" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019e4114-5597-7a99-addc-a5e2555978b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a99-addc-a5e2555978b7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019e4114-5597-7a99-addc-a5e2555978b7" xlink:to="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a99-addc-a5e2555978b7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019e4114-5597-7a99-addc-a5e2555978b7" xlink:to="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_019e4114-5597-7f5a-971f-41214d97891d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:to="loc_us-gaap_CommonStockMember_019e4114-5597-7f5a-971f-41214d97891d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_019e4114-5597-79d4-84d0-1782e1ff2201" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_019e4114-5597-79d4-84d0-1782e1ff2201" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_019e4114-5597-71ac-b1bb-771400277381" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:to="loc_us-gaap_RetainedEarningsMember_019e4114-5597-71ac-b1bb-771400277381" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019e4114-5597-7835-97f2-d4e75c88a3b6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019e4114-5597-7835-97f2-d4e75c88a3b6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_019e4114-5597-7fa5-b817-2248e8c34623" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:to="loc_us-gaap_TreasuryStockCommonMember_019e4114-5597-7fa5-b817-2248e8c34623" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockIncludingAdditionalPaidInCapitalMember_019e84c1-374c-7603-8379-1662cc034195" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockIncludingAdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-5597-7a62-b313-07015f4d9b0b" xlink:to="loc_us-gaap_CommonStockIncludingAdditionalPaidInCapitalMember_019e84c1-374c-7603-8379-1662cc034195" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019e4114-5597-75a7-833d-685cc1db9f56" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_019e4114-5597-75a7-833d-685cc1db9f56" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019e4114-5597-7a65-9782-3dee6da4c963" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockholdersEquity_019e4114-5597-7a65-9782-3dee6da4c963" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e4114-5597-78e4-9bc1-97d5b47e8fea" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_NetIncomeLoss_019e4114-5597-78e4-9bc1-97d5b47e8fea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_019e4114-5597-77a5-953f-a2ffcfa3447e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_019e4114-5597-77a5-953f-a2ffcfa3447e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DividendsCommonStockCash_019e4114-5597-726b-93bb-33b721f88fff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DividendsCommonStockCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_DividendsCommonStockCash_019e4114-5597-726b-93bb-33b721f88fff" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_019e4114-5597-7f6e-bb34-29e7570918d3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_019e4114-5597-7f6e-bb34-29e7570918d3" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueAcquisitions_019e4114-5597-7cdf-ac17-c5a63257099d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueAcquisitions_019e4114-5597-7cdf-ac17-c5a63257099d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_019e4114-5597-7ef7-bec1-9af11f651d9c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_019e4114-5597-7ef7-bec1-9af11f651d9c" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_019e4114-5597-7124-baf4-bf4495646eff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_019e4114-5597-7124-baf4-bf4495646eff" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_019e4114-5597-797f-8a71-cca1b5225eb2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockValueAcquiredCostMethod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_TreasuryStockValueAcquiredCostMethod_019e4114-5597-797f-8a71-cca1b5225eb2" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_019e4114-5597-7aab-a32f-cff2a1eeb9cb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_019e4114-5597-7aab-a32f-cff2a1eeb9cb" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_019e4114-5597-796b-a558-d89b01e71cf1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_019e4114-5597-796b-a558-d89b01e71cf1" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_StockIssuedDuringPeriodSharesDirectorStockPurchasePlans_019e4114-5597-7610-951b-0409f910120a" xlink:href="mpb-20260630.xsd#mpb_StockIssuedDuringPeriodSharesDirectorStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_mpb_StockIssuedDuringPeriodSharesDirectorStockPurchasePlans_019e4114-5597-7610-951b-0409f910120a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_StockIssuedDuringPeriodValueDirectorStockPurchasePlan_019e4114-5597-7665-98a2-98b64086df3b" xlink:href="mpb-20260630.xsd#mpb_StockIssuedDuringPeriodValueDirectorStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_mpb_StockIssuedDuringPeriodValueDirectorStockPurchasePlan_019e4114-5597-7665-98a2-98b64086df3b" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_019e4114-5597-7d52-9d10-43a9b3d8a842" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_019e4114-5597-7d52-9d10-43a9b3d8a842" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_019fa4ee-0833-777d-8713-75715932ca77" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_019fa4ee-0833-777d-8713-75715932ca77" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationRestrictedStockUnitsRequisiteServicePeriodRecognition_019e4114-5597-7b73-8739-d3b23922abd3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationRestrictedStockUnitsRequisiteServicePeriodRecognition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-5597-7637-bcce-7965dd95ef8c" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationRestrictedStockUnitsRequisiteServicePeriodRecognition_019e4114-5597-7b73-8739-d3b23922abd3" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019e4114-5597-7966-8f11-bd3f19428903" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019e4114-5597-7a54-bbfc-b4d7311723de" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/BusinessCombinationsNarrativeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#BusinessCombinationsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/BusinessCombinationsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7281-9dc8-1b35a767e497" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7281-9dc8-1b35a767e497" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-744c-92b7-b6af20016f68" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7281-9dc8-1b35a767e497" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-744c-92b7-b6af20016f68" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-744c-92b7-b6af20016f68_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-744c-92b7-b6af20016f68" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-744c-92b7-b6af20016f68_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7a92-bc5b-cc06d78b42fc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-744c-92b7-b6af20016f68" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7a92-bc5b-cc06d78b42fc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CumberlandAdvisorsInc.Member_019e4114-566f-7b7d-832a-bed0cdad519d" xlink:href="mpb-20260630.xsd#mpb_CumberlandAdvisorsInc.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7a92-bc5b-cc06d78b42fc" xlink:to="loc_mpb_CumberlandAdvisorsInc.Member_019e4114-566f-7b7d-832a-bed0cdad519d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A1stColonialAcquisitionMember_019eb712-fa39-760e-b420-d9fac18938e6" xlink:href="mpb-20260630.xsd#mpb_A1stColonialAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7a92-bc5b-cc06d78b42fc" xlink:to="loc_mpb_A1stColonialAcquisitionMember_019eb712-fa39-760e-b420-d9fac18938e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_WilliamPennAcquisitionMember_019e4114-566f-7acf-9e30-81bec992de1b" xlink:href="mpb-20260630.xsd#mpb_WilliamPennAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7a92-bc5b-cc06d78b42fc" xlink:to="loc_mpb_WilliamPennAcquisitionMember_019e4114-566f-7acf-9e30-81bec992de1b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CharisInsuranceGroupIncMember_019e4114-566f-7cf2-ab73-dcb693f7f3a8" xlink:href="mpb-20260630.xsd#mpb_CharisInsuranceGroupIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7a92-bc5b-cc06d78b42fc" xlink:to="loc_mpb_CharisInsuranceGroupIncMember_019e4114-566f-7cf2-ab73-dcb693f7f3a8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019eb716-019c-7bba-b9e0-aac6d4787cf3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7281-9dc8-1b35a767e497" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019eb716-019c-7bba-b9e0-aac6d4787cf3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019eb716-019c-7bba-b9e0-aac6d4787cf3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019eb716-019c-7bba-b9e0-aac6d4787cf3" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019eb716-019c-7bba-b9e0-aac6d4787cf3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019eb716-019c-7ae5-b80f-f4d719466129" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019eb716-019c-7bba-b9e0-aac6d4787cf3" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019eb716-019c-7ae5-b80f-f4d719466129" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerListsMember_019eb716-3041-7612-b6ac-f8c58720f67d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CustomerListsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019eb716-019c-7ae5-b80f-f4d719466129" xlink:to="loc_us-gaap_CustomerListsMember_019eb716-3041-7612-b6ac-f8c58720f67d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditLossStatusAxis_019e7338-d3eb-78a4-953d-aac26a7ddd99" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditLossStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7281-9dc8-1b35a767e497" xlink:to="loc_us-gaap_CreditLossStatusAxis_019e7338-d3eb-78a4-953d-aac26a7ddd99" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditLossStatusDomain_019e7338-d3eb-78a4-953d-aac26a7ddd99_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditLossStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditLossStatusAxis_019e7338-d3eb-78a4-953d-aac26a7ddd99" xlink:to="loc_us-gaap_CreditLossStatusDomain_019e7338-d3eb-78a4-953d-aac26a7ddd99_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditLossStatusDomain_019e7338-d3eb-7880-8701-074d1d4ea537" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditLossStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditLossStatusAxis_019e7338-d3eb-78a4-953d-aac26a7ddd99" xlink:to="loc_us-gaap_CreditLossStatusDomain_019e7338-d3eb-7880-8701-074d1d4ea537" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetAcquiredAndNoCreditDeteriorationMember_019e7339-0a7a-795b-b27e-2386bd1eea2f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialAssetAcquiredAndNoCreditDeteriorationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditLossStatusDomain_019e7338-d3eb-7880-8701-074d1d4ea537" xlink:to="loc_us-gaap_FinancialAssetAcquiredAndNoCreditDeteriorationMember_019e7339-0a7a-795b-b27e-2386bd1eea2f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetAcquiredWithCreditDeteriorationMember_019e733b-0788-7e72-a489-e811da86c2b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialAssetAcquiredWithCreditDeteriorationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditLossStatusDomain_019e7338-d3eb-7880-8701-074d1d4ea537" xlink:to="loc_us-gaap_FinancialAssetAcquiredWithCreditDeteriorationMember_019e733b-0788-7e72-a489-e811da86c2b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesAxis_019fc7b0-5cd0-7567-8846-d16ea3f1dfca" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ConsolidatedEntitiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7281-9dc8-1b35a767e497" xlink:to="loc_srt_ConsolidatedEntitiesAxis_019fc7b0-5cd0-7567-8846-d16ea3f1dfca" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_019fc7b0-5cd0-7567-8846-d16ea3f1dfca_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidatedEntitiesAxis_019fc7b0-5cd0-7567-8846-d16ea3f1dfca" xlink:to="loc_srt_ConsolidatedEntitiesDomain_019fc7b0-5cd0-7567-8846-d16ea3f1dfca_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidatedEntitiesDomain_019fc7b0-5cd0-7c82-bb91-7fe0132811be" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ConsolidatedEntitiesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidatedEntitiesAxis_019fc7b0-5cd0-7567-8846-d16ea3f1dfca" xlink:to="loc_srt_ConsolidatedEntitiesDomain_019fc7b0-5cd0-7c82-bb91-7fe0132811be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SubsidiariesMember_019fc7b0-b0fe-7be3-960e-0b3eb7bd007a" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_SubsidiariesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidatedEntitiesDomain_019fc7b0-5cd0-7c82-bb91-7fe0132811be" xlink:to="loc_srt_SubsidiariesMember_019fc7b0-b0fe-7be3-960e-0b3eb7bd007a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_019e4114-566f-73b4-a6d5-f46d60f7c798" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_019e4114-566f-73b4-a6d5-f46d60f7c798" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_019e7317-e295-7eeb-a8f8-dbe6185a6e23" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_019e7317-e295-7eeb-a8f8-dbe6185a6e23" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_019e4114-566f-7131-885f-6ca859e58bb6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_019e4114-566f-7131-885f-6ca859e58bb6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsUnderManagementCarryingAmount_019fc7b1-4137-75ff-a848-60de144b168c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsUnderManagementCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_AssetsUnderManagementCarryingAmount_019fc7b1-4137-75ff-a848-60de144b168c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_019e4114-566f-7678-aaed-60772703e2ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_Goodwill_019e4114-566f-7678-aaed-60772703e2ec" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_019e7335-1991-753a-883a-19a4bce2c36d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_019e7335-1991-753a-883a-19a4bce2c36d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_019e4114-566f-790d-884d-1a50691d4da8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationAcquisitionRelatedCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessCombinationAcquisitionRelatedCosts_019e4114-566f-790d-884d-1a50691d4da8" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivablePurchasedSeasonedLoansAmountAtPurchasedPrice_019e7337-ce03-78e7-a09a-81ef018706c2" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivablePurchasedSeasonedLoansAmountAtPurchasedPrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_mpb_FinancingReceivablePurchasedSeasonedLoansAmountAtPurchasedPrice_019e7337-ce03-78e7-a09a-81ef018706c2" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAmountAtPurchasePrice_019e7339-8bac-7ceb-b65c-fa9beac2ab3c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAmountAtPurchasePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAmountAtPurchasePrice_019e7339-8bac-7ceb-b65c-fa9beac2ab3c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableAllowanceForCreditLossAtAcquisitionDate_019e733d-d546-7ac3-a492-a8222f85e3f8" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableAllowanceForCreditLossAtAcquisitionDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_mpb_FinancingReceivableAllowanceForCreditLossAtAcquisitionDate_019e733d-d546-7ac3-a492-a8222f85e3f8" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate_019e733f-4a30-739a-9397-ecccaa8efe2b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate_019e733f-4a30-739a-9397-ecccaa8efe2b" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationProFormaInformationRevenueOfAcquireeSinceAcquisitionDateActual_019e4114-566f-7ed8-a949-a8cf3d2c47d0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationProFormaInformationRevenueOfAcquireeSinceAcquisitionDateActual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessCombinationProFormaInformationRevenueOfAcquireeSinceAcquisitionDateActual_019e4114-566f-7ed8-a949-a8cf3d2c47d0" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationProFormaInformationEarningsOrLossOfAcquireeSinceAcquisitionDateActual_019e4114-566f-7d2d-9755-f5a157df66ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationProFormaInformationEarningsOrLossOfAcquireeSinceAcquisitionDateActual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessCombinationProFormaInformationEarningsOrLossOfAcquireeSinceAcquisitionDateActual_019e4114-566f-7d2d-9755-f5a157df66ec" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionPercentageOfVotingInterestsAcquired_019e4114-566f-7681-b28f-0d30f801bf05" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionPercentageOfVotingInterestsAcquired"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_us-gaap_BusinessAcquisitionPercentageOfVotingInterestsAcquired_019e4114-566f-7681-b28f-0d30f801bf05" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationAdditionalBranches_019e4114-566f-7459-a177-111426fb9113" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationAdditionalBranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-79ab-b26d-fe5b6933a365" xlink:to="loc_mpb_BusinessCombinationAdditionalBranches_019e4114-566f-7459-a177-111426fb9113" xlink:type="arc" order="14"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/BusinessCombinationsScheduleofEstimatedFairValueofAssetsAcquiredDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#BusinessCombinationsScheduleofEstimatedFairValueofAssetsAcquiredDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/BusinessCombinationsScheduleofEstimatedFairValueofAssetsAcquiredDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-7aad-ae68-662f3092b10b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7bec-b08a-04f3d3699821" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-7aad-ae68-662f3092b10b" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7bec-b08a-04f3d3699821" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-73e8-b717-2898ba116037" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-7bec-b08a-04f3d3699821" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-73e8-b717-2898ba116037" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-73e8-b717-2898ba116037_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-73e8-b717-2898ba116037" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-73e8-b717-2898ba116037_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-71b4-aaf9-9eff8801f700" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-73e8-b717-2898ba116037" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-71b4-aaf9-9eff8801f700" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A1stColonialAcquisitionMember_019eb714-197f-7f14-b6b2-053dc8fba471" xlink:href="mpb-20260630.xsd#mpb_A1stColonialAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-71b4-aaf9-9eff8801f700" xlink:to="loc_mpb_A1stColonialAcquisitionMember_019eb714-197f-7f14-b6b2-053dc8fba471" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-7aad-ae68-662f3092b10b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents_019e4114-566f-74f6-974a-187f43be1e69" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashAndEquivalents_019e4114-566f-74f6-974a-187f43be1e69" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFederalFundsSold_019e4114-566f-7e3e-b3d4-26aa06194d0d" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFederalFundsSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFederalFundsSold_019e4114-566f-7e3e-b3d4-26aa06194d0d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsMarketableSecurities_019e4114-566f-7ec9-bd90-27732127359a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsMarketableSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsMarketableSecurities_019e4114-566f-7ec9-bd90-27732127359a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedAssetAcquiredLoansHeldForSale_019e4114-566f-7ba7-8c07-d0e3add17bfc" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedAssetAcquiredLoansHeldForSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_mpb_BusinessCombinationRecognizedAssetAcquiredLoansHeldForSale_019e4114-566f-7ba7-8c07-d0e3add17bfc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFinancialAssets_019f043e-2578-7576-a976-35c41d1cfa12" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFinancialAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedFinancialAssets_019f043e-2578-7576-a976-35c41d1cfa12" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_019e4114-566f-78df-8b0c-998f44381380" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedIntangibles_019e4114-566f-78df-8b0c-998f44381380" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_019e4114-566f-7013-876d-39fe803ae296" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedPropertyPlantAndEquipment_019e4114-566f-7013-876d-39fe803ae296" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOperatingLeaseRightOfUseAsset_019e4114-566f-748e-a86e-8c146cd0d4e1" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOperatingLeaseRightOfUseAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOperatingLeaseRightOfUseAsset_019e4114-566f-748e-a86e-8c146cd0d4e1" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashSurrenderValueOfLifeInsurance_019e4114-566f-7fa8-b08e-093b81662a6c" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashSurrenderValueOfLifeInsurance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCashSurrenderValueOfLifeInsurance_019e4114-566f-7fa8-b08e-093b81662a6c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets_019e4114-566f-7d34-9a82-ce597f7e7799" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedDeferredTaxAssets_019e4114-566f-7d34-9a82-ce597f7e7799" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_019e4114-566f-7be3-9772-8ac293632097" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentAssetsReceivables_019e4114-566f-7be3-9772-8ac293632097" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOtherNoncurrentAssets_019e4114-566f-7e2c-a4b6-4de258dba1b5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOtherNoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedOtherNoncurrentAssets_019e4114-566f-7e2c-a4b6-4de258dba1b5" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_019eb6fa-85e1-7f4c-8766-6b028c984473" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssetsAbstract_019e4114-566f-74ec-8dba-f4dc6c291f6d" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedAssets_019eb6fa-85e1-7f4c-8766-6b028c984473" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-7aad-ae68-662f3092b10b" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepositsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNonInterestBearingDemandDepositLiabilities_019e4114-566f-7d5d-8de9-2f0edfb689b0" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNonInterestBearingDemandDepositLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNonInterestBearingDemandDepositLiabilities_019e4114-566f-7d5d-8de9-2f0edfb689b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInterestBearingDemandDepositLiabilities_019e4114-566f-749f-9f01-182914ba5562" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInterestBearingDemandDepositLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedInterestBearingDemandDepositLiabilities_019e4114-566f-749f-9f01-182914ba5562" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedMoneyMarketDepositLiabilities_019e4114-566f-7c27-b813-82e295c16fd1" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedMoneyMarketDepositLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedMoneyMarketDepositLiabilities_019e4114-566f-7c27-b813-82e295c16fd1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedSavingsDepositLiabilities_019e4114-566f-77b2-b90a-d03080cbc7c8" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedSavingsDepositLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedSavingsDepositLiabilities_019e4114-566f-77b2-b90a-d03080cbc7c8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedTimeDepositLiabilities_019e4114-566f-748e-938d-9ced675f626f" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedTimeDepositLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositsAbstract_019e4114-566f-7673-95d5-abb9795e5867" xlink:to="loc_mpb_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedTimeDepositLiabilities_019e4114-566f-748e-938d-9ced675f626f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCapitalLeaseObligation_019e4114-566f-7927-a271-a54d2b70b848" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCapitalLeaseObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCapitalLeaseObligation_019e4114-566f-7927-a271-a54d2b70b848" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_019e4114-566f-7939-b458-fd72270cc661" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedCurrentLiabilitiesAccountsPayable_019e4114-566f-7939-b458-fd72270cc661" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther_019e4114-566f-7bca-9bd9-0365e9cea7ed" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesOther_019e4114-566f-7bca-9bd9-0365e9cea7ed" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities_019e4114-566f-741d-a412-b579e4d3dcc1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilities_019e4114-566f-741d-a412-b579e4d3dcc1" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferred1_019e4114-566f-7b2f-b23d-00ef3781bd30" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationConsiderationTransferred1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferred1_019e4114-566f-7b2f-b23d-00ef3781bd30" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationCashPaidInLieuOfFractionalShares_019e7355-fc2a-7403-91f3-a38b10660751" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationCashPaidInLieuOfFractionalShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_mpb_BusinessCombinationCashPaidInLieuOfFractionalShares_019e7355-fc2a-7403-91f3-a38b10660751" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesGross_019e4114-566f-7de8-9f46-741921e4cce5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquireBusinessesGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesGross_019e4114-566f-7de8-9f46-741921e4cce5" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationPurchasePriceAssignedToStockOptionsSettledForCash_019e7357-16f0-77b5-a997-abfc9908c096" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationPurchasePriceAssignedToStockOptionsSettledForCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_mpb_BusinessCombinationPurchasePriceAssignedToStockOptionsSettledForCash_019e7357-16f0-77b5-a997-abfc9908c096" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_019e4114-566f-7cd3-bfd1-d02e7fef2c2a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationConsiderationTransferredEquityInterestsIssuedAndIssuable_019e4114-566f-7cd3-bfd1-d02e7fef2c2a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_019e4114-566f-7f8e-bb67-8c97a02760be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNet_019e4114-566f-7f8e-bb67-8c97a02760be" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_019e4114-566f-7541-b072-302e2a9fd985" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedLiabilitiesAbstract_019e4114-566f-771a-8297-6b7cde2b3b75" xlink:to="loc_us-gaap_Goodwill_019e4114-566f-7541-b072-302e2a9fd985" xlink:type="arc" order="12"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/BusinessCombinationsScheduleofSupplementalProFormaInformationDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#BusinessCombinationsScheduleofSupplementalProFormaInformationDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/BusinessCombinationsScheduleofSupplementalProFormaInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-75f2-86f9-6a4292f2952f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-72d4-8d44-c98481cda297" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-75f2-86f9-6a4292f2952f" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-72d4-8d44-c98481cda297" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7963-bd4b-1b1989220bfc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e4114-566f-72d4-8d44-c98481cda297" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7963-bd4b-1b1989220bfc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7963-bd4b-1b1989220bfc_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7963-bd4b-1b1989220bfc" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7963-bd4b-1b1989220bfc_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-70a9-a3c1-34bd95fb5f5d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7963-bd4b-1b1989220bfc" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-70a9-a3c1-34bd95fb5f5d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A1stColonialAcquisitionMember_019eb714-484d-7044-a6c1-326a224df9e7" xlink:href="mpb-20260630.xsd#mpb_A1stColonialAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-70a9-a3c1-34bd95fb5f5d" xlink:to="loc_mpb_A1stColonialAcquisitionMember_019eb714-484d-7044-a6c1-326a224df9e7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationProFormaInformationProFormaInterestIncomeExpenseAfterProvisionForLoanLoss_019e4114-566f-767a-8ae1-9c4c82760621" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationProFormaInformationProFormaInterestIncomeExpenseAfterProvisionForLoanLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-75f2-86f9-6a4292f2952f" xlink:to="loc_mpb_BusinessCombinationProFormaInformationProFormaInterestIncomeExpenseAfterProvisionForLoanLoss_019e4114-566f-767a-8ae1-9c4c82760621" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationProFormaInformationProFormaNoninterestIncome_019e4114-566f-750a-a926-3c01cd3edc67" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationProFormaInformationProFormaNoninterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-75f2-86f9-6a4292f2952f" xlink:to="loc_mpb_BusinessCombinationProFormaInformationProFormaNoninterestIncome_019e4114-566f-750a-a926-3c01cd3edc67" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationProFormaInformationProFormaNoninterestExpense_019e4114-566f-701b-a05a-d3f29e6412bb" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationProFormaInformationProFormaNoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-75f2-86f9-6a4292f2952f" xlink:to="loc_mpb_BusinessCombinationProFormaInformationProFormaNoninterestExpense_019e4114-566f-701b-a05a-d3f29e6412bb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionsProFormaNetIncomeLoss_019e4114-566f-71a1-a6f9-dd520011e57e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionsProFormaNetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e4114-566f-75f2-86f9-6a4292f2952f" xlink:to="loc_us-gaap_BusinessAcquisitionsProFormaNetIncomeLoss_019e4114-566f-71a1-a6f9-dd520011e57e" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/InvestmentSecuritiesScheduleofUnrealizedGainLossonInvestmentsDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#InvestmentSecuritiesScheduleofUnrealizedGainLossonInvestmentsDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/InvestmentSecuritiesScheduleofUnrealizedGainLossonInvestmentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfInvestmentsTable_019e4114-566f-7d31-93f7-a7a3f5638c00" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_ScheduleOfInvestmentsTable_019e4114-566f-7d31-93f7-a7a3f5638c00" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7665-a2e1-828379d77988" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfInvestmentsTable_019e4114-566f-7d31-93f7-a7a3f5638c00" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7665-a2e1-828379d77988" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7665-a2e1-828379d77988_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7665-a2e1-828379d77988" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7665-a2e1-828379d77988_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c14-867e-7aafe156c0d9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7665-a2e1-828379d77988" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c14-867e-7aafe156c0d9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_019e4114-566f-73e7-9bbd-0632ce7da774" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c14-867e-7aafe156c0d9" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_019e4114-566f-73e7-9bbd-0632ce7da774" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-566f-72c9-b547-089c0165cf52" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c14-867e-7aafe156c0d9" xlink:to="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-566f-72c9-b547-089c0165cf52" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-566f-765a-adb2-9c14f0d5bdf3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c14-867e-7aafe156c0d9" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-566f-765a-adb2-9c14f0d5bdf3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-566f-7304-b880-56b93ef34c1e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c14-867e-7aafe156c0d9" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-566f-7304-b880-56b93ef34c1e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesAbstract_019e4114-566f-7bb5-835a-8382898f2848" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesAbstract_019e4114-566f-7bb5-835a-8382898f2848" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-774f-8e00-7dca8bee409d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_019e4114-566f-7bb5-835a-8382898f2848" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-774f-8e00-7dca8bee409d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_019e4114-566f-7eee-9cb1-b7dd1e897e88" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_019e4114-566f-7bb5-835a-8382898f2848" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedGainBeforeTax_019e4114-566f-7eee-9cb1-b7dd1e897e88" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_019e4114-566f-7f68-9576-234f35831731" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_019e4114-566f-7bb5-835a-8382898f2848" xlink:to="loc_us-gaap_AvailableForSaleDebtSecuritiesAccumulatedGrossUnrealizedLossBeforeTax_019e4114-566f-7f68-9576-234f35831731" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-566f-7854-bc73-73ed176b7f42" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesAbstract_019e4114-566f-7bb5-835a-8382898f2848" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-566f-7854-bc73-73ed176b7f42" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract_019e4114-566f-75a9-b9fd-6936974af3e8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract_019e4114-566f-75a9-b9fd-6936974af3e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-727d-acd7-de0e6824b50b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract_019e4114-566f-75a9-b9fd-6936974af3e8" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-727d-acd7-de0e6824b50b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_019e4114-566f-746d-a9c7-40e6dff5a723" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract_019e4114-566f-75a9-b9fd-6936974af3e8" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingGain_019e4114-566f-746d-a9c7-40e6dff5a723" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_019e4114-566f-7bbd-8867-3bbb33ae768f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract_019e4114-566f-75a9-b9fd-6936974af3e8" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesAccumulatedUnrecognizedHoldingLoss_019e4114-566f-7bbd-8867-3bbb33ae768f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_019e4114-566f-7114-be64-525771542aca" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtSecuritiesHeldToMaturityFairValueAbstract_019e4114-566f-75a9-b9fd-6936974af3e8" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_019e4114-566f-7114-be64-525771542aca" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityAmortizedCostBeforeAllowanceForCreditLoss_019e4114-566f-7dde-9cb7-8516b5a185ea" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityAmortizedCostBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityAmortizedCostBeforeAllowanceForCreditLoss_019e4114-566f-7dde-9cb7-8516b5a185ea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityCumulativeUnrecognizedGain_019e4114-566f-7d5d-9d1f-e6bce1ab809a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityCumulativeUnrecognizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityCumulativeUnrecognizedGain_019e4114-566f-7d5d-9d1f-e6bce1ab809a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityCumulativeUnrecognizedLoss_019e4114-566f-7a12-9b81-b292543aefe9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityCumulativeUnrecognizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityCumulativeUnrecognizedLoss_019e4114-566f-7a12-9b81-b292543aefe9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityFairValue_019e4114-566f-76d6-a353-32de58f42e67" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7275-b410-e663cbdef3e5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAndHeldToMaturityFairValue_019e4114-566f-76d6-a353-32de58f42e67" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/InvestmentSecuritiesScheduleofFairValueandUnrealizedLossonDebtSecurityInvestmentsinaContinuousUnrealizedLossPositionDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#InvestmentSecuritiesScheduleofFairValueandUnrealizedLossonDebtSecurityInvestmentsinaContinuousUnrealizedLossPositionDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/InvestmentSecuritiesScheduleofFairValueandUnrealizedLossonDebtSecurityInvestmentsinaContinuousUnrealizedLossPositionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_019e4114-566f-763a-8763-41bff3000f8f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_019e4114-566f-763a-8763-41bff3000f8f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-77f7-8f85-7e565e226c04" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_019e4114-566f-763a-8763-41bff3000f8f" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-77f7-8f85-7e565e226c04" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-77f7-8f85-7e565e226c04_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-77f7-8f85-7e565e226c04" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-77f7-8f85-7e565e226c04_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7b38-8aa4-2c801cb02eb3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-77f7-8f85-7e565e226c04" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7b38-8aa4-2c801cb02eb3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_019e4114-566f-7f52-9dfe-3d372c3cee79" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7b38-8aa4-2c801cb02eb3" xlink:to="loc_us-gaap_USTreasuryAndGovernmentMember_019e4114-566f-7f52-9dfe-3d372c3cee79" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-566f-7485-a926-0c3de103a98a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7b38-8aa4-2c801cb02eb3" xlink:to="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-566f-7485-a926-0c3de103a98a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-566f-7df0-99a4-3bdcf5322e42" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7b38-8aa4-2c801cb02eb3" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-566f-7df0-99a4-3bdcf5322e42" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-566f-7de9-bda8-390a891a7db2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7b38-8aa4-2c801cb02eb3" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-566f-7de9-bda8-390a891a7db2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsNumberOfPositions_019e4114-566f-7cad-90fe-dc4d3833023e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsNumberOfPositions_019e4114-566f-7cad-90fe-dc4d3833023e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_019e4114-566f-7337-b919-8775d233e8ca" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12Months_019e4114-566f-7337-b919-8775d233e8ca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_019e4114-566f-766c-a52e-0d5c93639575" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_019e4114-566f-766c-a52e-0d5c93639575" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerNumberOfPositions_019e4114-566f-7a56-b978-acd464de23e6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerNumberOfPositions_019e4114-566f-7a56-b978-acd464de23e6" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_019e4114-566f-7b51-9d9c-3c69bd76a3bb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLonger_019e4114-566f-7b51-9d9c-3c69bd76a3bb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_019e4114-566f-7b05-afb5-5ef7a36d50bd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_019e4114-566f-7b05-afb5-5ef7a36d50bd" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions_019e4114-566f-79b4-9980-293132d1f5e4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionNumberOfPositions_019e4114-566f-79b4-9980-293132d1f5e4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_019e4114-566f-74b6-928e-c38f5d89a5f6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPosition_019e4114-566f-74b6-928e-c38f5d89a5f6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_019e4114-566f-72af-9eef-1c111d913134" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_019e4114-566f-797d-84a1-0c569f6306a5" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleUnrealizedLossPositionAccumulatedLoss_019e4114-566f-72af-9eef-1c111d913134" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_019e4114-566f-7d63-95d0-a31be4c4131b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfHeldToMaturitySecuritiesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_019e4114-566f-7d63-95d0-a31be4c4131b" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7008-ab80-8d835421fc82" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesTable_019e4114-566f-7d63-95d0-a31be4c4131b" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7008-ab80-8d835421fc82" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7008-ab80-8d835421fc82_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7c34-a719-a532981cc943" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasuryAndGovernmentMember_019e4114-566f-7bab-9513-78675805fd1a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USTreasuryAndGovernmentMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-566f-707d-9a68-061fa468ebe5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-566f-7857-a0db-45c1f9ee907b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-566f-7983-a80e-ab5aea5491a3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThanOneYear_019e4114-566f-7457-a626-69cc3cb4fde2" xlink:href="mpb-20260630.xsd#mpb_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThanOneYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_mpb_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsLessThanOneYear_019e4114-566f-7457-a626-69cc3cb4fde2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThanTwelveMonthsFairValue_019e4114-566f-773a-a323-5240ad6c646d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThanTwelveMonthsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThanTwelveMonthsFairValue_019e4114-566f-773a-a323-5240ad6c646d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_019e4114-566f-7679-a7a6-ef4d46710a21" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionLessThan12MonthsAccumulatedLoss_019e4114-566f-7679-a7a6-ef4d46710a21" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsGreaterThanOrEqualToOneYear_019e4114-566f-7933-9614-5a44867231d3" xlink:href="mpb-20260630.xsd#mpb_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsGreaterThanOrEqualToOneYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_mpb_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositionsGreaterThanOrEqualToOneYear_019e4114-566f-7933-9614-5a44867231d3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionTwelveMonthsOrLongerFairValue_019e4114-566f-7beb-8365-e9cfdfe3c949" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionTwelveMonthsOrLongerFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionTwelveMonthsOrLongerFairValue_019e4114-566f-7beb-8365-e9cfdfe3c949" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_019e4114-566f-7680-8b0b-963724db7845" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPosition12MonthsOrLongerAccumulatedLoss_019e4114-566f-7680-8b0b-963724db7845" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions_019e4114-566f-7b7d-8c6b-20d27fbc9640" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesInUnrealizedLossPositionsQualitativeDisclosureNumberOfPositions_019e4114-566f-7b7d-8c6b-20d27fbc9640" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionFairValue_019e4114-566f-73a8-bc40-3b2d6c4d43b1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionFairValue_019e4114-566f-73a8-bc40-3b2d6c4d43b1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionAccumulatedLoss_019e4114-566f-7012-834b-5902df949806" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionAccumulatedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfHeldToMaturitySecuritiesLineItems_019e4114-566f-7daa-94e3-adb3f7f0e722" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesContinuousUnrealizedLossPositionAccumulatedLoss_019e4114-566f-7012-834b-5902df949806" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/InvestmentSecuritiesScheduleofInvestmentsClassifiedbyContractualMaturityDateDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#InvestmentSecuritiesScheduleofInvestmentsClassifiedbyContractualMaturityDateDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/InvestmentSecuritiesScheduleofInvestmentsClassifiedbyContractualMaturityDateDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7097-933e-475231ec944d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfInvestmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfInvestmentsTable_019e4114-566f-718e-bfe9-8ef0c8da3eda" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfInvestmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7097-933e-475231ec944d" xlink:to="loc_us-gaap_ScheduleOfInvestmentsTable_019e4114-566f-718e-bfe9-8ef0c8da3eda" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-757b-966b-141a9f3335ab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfInvestmentsTable_019e4114-566f-718e-bfe9-8ef0c8da3eda" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-757b-966b-141a9f3335ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-757b-966b-141a9f3335ab_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-757b-966b-141a9f3335ab" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-757b-966b-141a9f3335ab_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7ec4-b760-5effb5490381" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-757b-966b-141a9f3335ab" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7ec4-b760-5effb5490381" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBackedSecuritiesMember_019e4114-566f-70f2-83d9-677241325bf5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7ec4-b760-5effb5490381" xlink:to="loc_us-gaap_MortgageBackedSecuritiesMember_019e4114-566f-70f2-83d9-677241325bf5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAbstract_019e4114-566f-7c4c-a943-361efdda0758" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7097-933e-475231ec944d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAbstract_019e4114-566f-7c4c-a943-361efdda0758" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAbstract_019e4114-566f-7c4c-a943-361efdda0758" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearAmortizedCost_019e4114-566f-76b6-8d2b-b37575bc7bed" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearAmortizedCost_019e4114-566f-76b6-8d2b-b37575bc7bed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsAmortizedCost_019e4114-566f-7d59-b19e-f67e78203be9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsAmortizedCost_019e4114-566f-7d59-b19e-f67e78203be9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterFiveThroughTenYearsAmortizedCost_019e4114-566f-71b2-9d16-88fcc02d01cd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterFiveThroughTenYearsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterFiveThroughTenYearsAmortizedCost_019e4114-566f-71b2-9d16-88fcc02d01cd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterTenYearsAmortizedCost_019e4114-566f-7818-a749-bb22758681ea" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterTenYearsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterTenYearsAmortizedCost_019e4114-566f-7818-a749-bb22758681ea" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesSingleMaturityDateAmortizedCostBasis_019e4114-566f-75ec-adda-c2b1c0e3f76a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesSingleMaturityDateAmortizedCostBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesSingleMaturityDateAmortizedCostBasis_019e4114-566f-75ec-adda-c2b1c0e3f76a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithoutSingleMaturityDateAmortizedCost_019e4114-566f-7749-a994-b60507bce5b4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithoutSingleMaturityDateAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithoutSingleMaturityDateAmortizedCost_019e4114-566f-7749-a994-b60507bce5b4" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-7fc5-a7a4-cc1ae804abed" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAmortizedCostAbstract_019e4114-566f-7a77-bb00-b85080920d9f" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleAmortizedCostExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-7fc5-a7a4-cc1ae804abed" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAbstract_019e4114-566f-7c4c-a943-361efdda0758" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearFairValue_019e4114-566f-7612-a881-6a042ee56bf3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearFairValue_019e4114-566f-7612-a881-6a042ee56bf3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue_019e4114-566f-73a5-aae9-2a4b9ac6554b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue_019e4114-566f-73a5-aae9-2a4b9ac6554b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterFiveThroughTenYearsFairValue_019e4114-566f-7551-a686-46174d0a5de0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterFiveThroughTenYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterFiveThroughTenYearsFairValue_019e4114-566f-7551-a686-46174d0a5de0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterTenYearsFairValue_019e4114-566f-7256-874e-921dd1d7bac2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterTenYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterTenYearsFairValue_019e4114-566f-7256-874e-921dd1d7bac2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesSingleMaturityDate_019e4114-566f-7bb4-a4b5-3d3890a0c056" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesSingleMaturityDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesSingleMaturityDate_019e4114-566f-7bb4-a4b5-3d3890a0c056" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithoutSingleMaturityDateFairValue_019e4114-566f-7a6b-a099-f246db93a3e2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithoutSingleMaturityDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithoutSingleMaturityDateFairValue_019e4114-566f-7a6b-a099-f246db93a3e2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-566f-78d6-afaa-d6ec7e89cc52" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-751a-96b2-0630b166020d" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-566f-78d6-afaa-d6ec7e89cc52" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAbstract_019e4114-566f-766c-92f1-2989b7765207" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfInvestmentsLineItems_019e4114-566f-7097-933e-475231ec944d" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAbstract_019e4114-566f-766c-92f1-2989b7765207" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAbstract_019e4114-566f-766c-92f1-2989b7765207" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateYearOne_019e4114-566f-7684-9faf-f0995b81dd87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateYearOne"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateYearOne_019e4114-566f-7684-9faf-f0995b81dd87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYearOneThroughFive_019e4114-566f-7901-994d-8f5673f201a2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYearOneThroughFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYearOneThroughFive_019e4114-566f-7901-994d-8f5673f201a2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYear5Through10_019e4114-566f-794f-b15e-4aa96159384e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYear5Through10"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYear5Through10_019e4114-566f-794f-b15e-4aa96159384e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYear10_019e4114-566f-7b17-a73d-d628b46964d9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYear10"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDateAfterYear10_019e4114-566f-7b17-a73d-d628b46964d9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDate_019e4114-566f-7066-937a-5f1bd95f5f30" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityAllocatedAndSingleMaturityDate_019e4114-566f-7066-937a-5f1bd95f5f30" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityWithoutSingleMaturityDate_019e4114-566f-7c4f-b5f4-4c034e3bb3d3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityWithoutSingleMaturityDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityAmortizedCostExcludingAccruedInterestAfterAllowanceForCreditLossMaturityWithoutSingleMaturityDate_019e4114-566f-7c4f-b5f4-4c034e3bb3d3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesHeldToMaturityExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-73c9-a729-77d5bc5feded" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesHeldToMaturityExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesNetCarryingAmountAbstract_019e4114-566f-7cc1-a874-211c8bf92361" xlink:to="loc_us-gaap_DebtSecuritiesHeldToMaturityExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-73c9-a729-77d5bc5feded" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAbstract_019e4114-566f-766c-92f1-2989b7765207" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearFairValue_019e4114-566f-7532-b845-1cb6127bb4be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithinOneYearFairValue_019e4114-566f-7532-b845-1cb6127bb4be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue_019e4114-566f-77a7-865c-55e90a7c4d57" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue_019e4114-566f-77a7-865c-55e90a7c4d57" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterFiveThroughTenYearsFairValue_019e4114-566f-7279-aced-319a816f58cf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterFiveThroughTenYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterFiveThroughTenYearsFairValue_019e4114-566f-7279-aced-319a816f58cf" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterTenYearsFairValue_019e4114-566f-796f-96b1-d37dac68048b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterTenYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesAfterTenYearsFairValue_019e4114-566f-796f-96b1-d37dac68048b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesSingleMaturityDateFairValue_019e4114-566f-79cd-a26c-2fe9a0d9d5ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesSingleMaturityDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesSingleMaturityDateFairValue_019e4114-566f-79cd-a26c-2fe9a0d9d5ec" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithoutSingleMaturityDateFairValue_019e4114-566f-7607-9f3c-a881d94262ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithoutSingleMaturityDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesWithoutSingleMaturityDateFairValue_019e4114-566f-7607-9f3c-a881d94262ec" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_019e4114-566f-7950-85be-05c04b095d81" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HeldToMaturitySecuritiesDebtMaturitiesFairValueAbstract_019e4114-566f-7fcd-9e9e-b04fece2d4ba" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_019e4114-566f-7950-85be-05c04b095d81" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofClassesoftheLoanPortfolioDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansScheduleofClassesoftheLoanPortfolioDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofClassesoftheLoanPortfolioDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7100-b123-b4138d277925" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-7fee-9892-1b123604d15b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7100-b123-b4138d277925" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-7fee-9892-1b123604d15b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7519-8fc3-e7baa11cd685" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-7fee-9892-1b123604d15b" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7519-8fc3-e7baa11cd685" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7519-8fc3-e7baa11cd685_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7519-8fc3-e7baa11cd685" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7519-8fc3-e7baa11cd685_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7519-8fc3-e7baa11cd685" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7ce9-856e-c8e204ebff33" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7ce9-856e-c8e204ebff33" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-73cc-9f4e-37a491900492" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-73cc-9f4e-37a491900492" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionLoansMember_019e4114-566f-74c7-9b4d-efa7c6b154cf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConstructionLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:to="loc_us-gaap_ConstructionLoansMember_019e4114-566f-74c7-9b4d-efa7c6b154cf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-758c-a0f4-538d63cd819e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-758c-a0f4-538d63cd819e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-723c-99a8-8a7a0b68ac84" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7009-9b8c-368f9c33b5c2" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-723c-99a8-8a7a0b68ac84" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7327-8653-1f13fd89c992" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-7fee-9892-1b123604d15b" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7327-8653-1f13fd89c992" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7327-8653-1f13fd89c992_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7327-8653-1f13fd89c992" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7327-8653-1f13fd89c992_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7327-8653-1f13fd89c992" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7e97-b92e-e6b997e312c9" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7e97-b92e-e6b997e312c9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-75ec-8c3a-6deb380601df" xlink:href="mpb-20260630.xsd#mpb_CREOwnerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-75ec-8c3a-6deb380601df" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MultifamilyMember_019e4114-566f-7bf8-addf-ce6c2884ab2e" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MultifamilyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_srt_MultifamilyMember_019e4114-566f-7bf8-addf-ce6c2884ab2e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FarmlandMember_019e4114-566f-7100-a247-b887fdd7ed4b" xlink:href="mpb-20260630.xsd#mpb_FarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_FarmlandMember_019e4114-566f-7100-a247-b887fdd7ed4b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ResidentialConstructionMember_019e4114-566f-7306-a777-3fefb1a96b3d" xlink:href="mpb-20260630.xsd#mpb_ResidentialConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_ResidentialConstructionMember_019e4114-566f-7306-a777-3fefb1a96b3d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OtherConstructionMember_019e4114-566f-7eaa-9c4f-b783a51f660e" xlink:href="mpb-20260630.xsd#mpb_OtherConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_OtherConstructionMember_019e4114-566f-7eaa-9c4f-b783a51f660e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14Family1stLienMember_019e4114-566f-7489-8f0f-d9027749e6d9" xlink:href="mpb-20260630.xsd#mpb_A14Family1stLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_A14Family1stLienMember_019e4114-566f-7489-8f0f-d9027749e6d9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019e4114-566f-7297-9b5b-73fb6415ed41" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_A14FamilyRentalMember_019e4114-566f-7297-9b5b-73fb6415ed41" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-7354-b74f-29c37dda5617" xlink:href="mpb-20260630.xsd#mpb_HELOCAndJuniorLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-77ec-8f72-da6a4c2600ac" xlink:to="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-7354-b74f-29c37dda5617" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-7188-9693-2ae14e70ea21" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7100-b123-b4138d277925" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-7188-9693-2ae14e70ea21" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansNarrativeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-7407-8bc5-6f55f8669edc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-7407-8bc5-6f55f8669edc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-78e0-ab59-32522a8e6016" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-7407-8bc5-6f55f8669edc" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-78e0-ab59-32522a8e6016" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-78e0-ab59-32522a8e6016_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-78e0-ab59-32522a8e6016" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-78e0-ab59-32522a8e6016_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-786b-b1ad-60c7142eea1a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-78e0-ab59-32522a8e6016" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-786b-b1ad-60c7142eea1a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBackedSecuritiesMember_019e4114-566f-79f6-a96b-0e63994393d6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBackedSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-786b-b1ad-60c7142eea1a" xlink:to="loc_us-gaap_MortgageBackedSecuritiesMember_019e4114-566f-79f6-a96b-0e63994393d6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableUnamortizedLoanCommitmentOriginationFeeAndPremiumDiscount_019e4114-566f-7854-a507-be3876f44dcb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableUnamortizedLoanCommitmentOriginationFeeAndPremiumDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableUnamortizedLoanCommitmentOriginationFeeAndPremiumDiscount_019e4114-566f-7854-a507-be3876f44dcb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAccruedInterestAfterAllowanceForCreditLoss_019e4114-566f-71cc-99d1-271ff46caa02" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAccruedInterestAfterAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableAccruedInterestAfterAllowanceForCreditLoss_019e4114-566f-71cc-99d1-271ff46caa02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAccruedInterestAfterAllowanceForCreditLossStatementOfFinancialPositionExtensibleList_019e4114-566f-793c-be5a-af65f3a9e2d5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAccruedInterestAfterAllowanceForCreditLossStatementOfFinancialPositionExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableAccruedInterestAfterAllowanceForCreditLossStatementOfFinancialPositionExtensibleList_019e4114-566f-793c-be5a-af65f3a9e2d5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableNonaccrualInterestIncome_019e4114-566f-7303-a7bc-d18144c0dd04" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableNonaccrualInterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableNonaccrualInterestIncome_019e4114-566f-7303-a7bc-d18144c0dd04" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageLoansInProcessOfForeclosureAmount_019e4114-566f-7a5a-87d0-70fc14116d76" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageLoansInProcessOfForeclosureAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_MortgageLoansInProcessOfForeclosureAmount_019e4114-566f-7a5a-87d0-70fc14116d76" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-74e9-8c69-d8d10db9a64d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-74e9-8c69-d8d10db9a64d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPeriod_019e4114-566f-704e-ab7a-93f440e7c9e0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPeriod_019e4114-566f-704e-ab7a-93f440e7c9e0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModifiedWeightedAverageTermIncreaseFromModification_019e4114-566f-7ff0-9074-e7be20c462d7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableModifiedWeightedAverageTermIncreaseFromModification"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableModifiedWeightedAverageTermIncreaseFromModification_019e4114-566f-7ff0-9074-e7be20c462d7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPast12Months_019e736a-9a96-739b-88a9-fa793e9bb2f6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPast12Months"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-7df4-a9b1-f713979cdc03" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPast12Months_019e736a-9a96-739b-88a9-fa793e9bb2f6" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofLoanPortfolioSummarizedbythePastDueStatusDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansScheduleofLoanPortfolioSummarizedbythePastDueStatusDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofLoanPortfolioSummarizedbythePastDueStatusDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7d1d-bbe1-192e600e3c5d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-7661-8cae-8ee8b1e13da2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivablesPastDueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7d1d-bbe1-192e600e3c5d" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-7661-8cae-8ee8b1e13da2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-704b-86c4-68feb080b140" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-7661-8cae-8ee8b1e13da2" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-704b-86c4-68feb080b140" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-704b-86c4-68feb080b140_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-704b-86c4-68feb080b140" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-704b-86c4-68feb080b140_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-704b-86c4-68feb080b140" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7199-9edf-8a6e9d66b207" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7199-9edf-8a6e9d66b207" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7557-9e31-104707ab683b" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7557-9e31-104707ab683b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionLoansMember_019e4114-566f-74ba-bb85-f4942e560ee3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConstructionLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:to="loc_us-gaap_ConstructionLoansMember_019e4114-566f-74ba-bb85-f4942e560ee3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-7427-895a-68f4c89da00a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-7427-895a-68f4c89da00a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-7399-97da-fecb409aa24e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d8e-905a-c55dc8e1ccfa" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-7399-97da-fecb409aa24e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_019e4114-566f-7ab8-888f-c2ee3a972bf3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablesPeriodPastDueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-7661-8cae-8ee8b1e13da2" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_019e4114-566f-7ab8-888f-c2ee3a972bf3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_019e4114-566f-7ab8-888f-c2ee3a972bf3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_019e4114-566f-7ab8-888f-c2ee3a972bf3" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_019e4114-566f-7ab8-888f-c2ee3a972bf3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_019e4114-566f-7e0a-8e3b-6cbab12da80e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablesPeriodPastDueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueAxis_019e4114-566f-7ab8-888f-c2ee3a972bf3" xlink:to="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_019e4114-566f-7e0a-8e3b-6cbab12da80e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetPastDueMember_019e4114-566f-7d59-9ede-7a06ebf5f1fb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialAssetPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_019e4114-566f-7e0a-8e3b-6cbab12da80e" xlink:to="loc_us-gaap_FinancialAssetPastDueMember_019e4114-566f-7d59-9ede-7a06ebf5f1fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivables30To59DaysPastDueMember_019e4114-566f-7f2d-9d90-fc0b303bc35e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivables30To59DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialAssetPastDueMember_019e4114-566f-7d59-9ede-7a06ebf5f1fb" xlink:to="loc_us-gaap_FinancingReceivables30To59DaysPastDueMember_019e4114-566f-7f2d-9d90-fc0b303bc35e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivables60To89DaysPastDueMember_019e4114-566f-7d36-87d0-a0eb2e63861e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivables60To89DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialAssetPastDueMember_019e4114-566f-7d59-9ede-7a06ebf5f1fb" xlink:to="loc_us-gaap_FinancingReceivables60To89DaysPastDueMember_019e4114-566f-7d36-87d0-a0eb2e63861e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember_019e4114-566f-7847-9442-c92a50eb57f1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialAssetPastDueMember_019e4114-566f-7d59-9ede-7a06ebf5f1fb" xlink:to="loc_us-gaap_FinancingReceivablesEqualToGreaterThan90DaysPastDueMember_019e4114-566f-7847-9442-c92a50eb57f1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialAssetNotPastDueMember_019e4114-566f-747b-b025-93a8ac15bb7a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialAssetNotPastDueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablesPeriodPastDueDomain_019e4114-566f-7e0a-8e3b-6cbab12da80e" xlink:to="loc_us-gaap_FinancialAssetNotPastDueMember_019e4114-566f-747b-b025-93a8ac15bb7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7b6a-ab78-65a24ab77101" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-7661-8cae-8ee8b1e13da2" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7b6a-ab78-65a24ab77101" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b6a-ab78-65a24ab77101_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7b6a-ab78-65a24ab77101" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b6a-ab78-65a24ab77101_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7b6a-ab78-65a24ab77101" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7e87-8687-228b0adcd140" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7e87-8687-228b0adcd140" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-7871-9153-83d1ffbe264f" xlink:href="mpb-20260630.xsd#mpb_CREOwnerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-7871-9153-83d1ffbe264f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MultifamilyMember_019e4114-566f-72e1-b567-06a8ba9c84a7" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MultifamilyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_srt_MultifamilyMember_019e4114-566f-72e1-b567-06a8ba9c84a7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FarmlandMember_019e4114-566f-70ee-8081-a0e6f595a3ab" xlink:href="mpb-20260630.xsd#mpb_FarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_FarmlandMember_019e4114-566f-70ee-8081-a0e6f595a3ab" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ResidentialConstructionMember_019e4114-566f-7c74-b139-cc873421eb07" xlink:href="mpb-20260630.xsd#mpb_ResidentialConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_ResidentialConstructionMember_019e4114-566f-7c74-b139-cc873421eb07" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OtherConstructionMember_019e4114-566f-79e4-a02b-57b22ed91f15" xlink:href="mpb-20260630.xsd#mpb_OtherConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_OtherConstructionMember_019e4114-566f-79e4-a02b-57b22ed91f15" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14Family1stLienMember_019e4114-566f-7317-b512-2003679e8a45" xlink:href="mpb-20260630.xsd#mpb_A14Family1stLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_A14Family1stLienMember_019e4114-566f-7317-b512-2003679e8a45" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019e4114-566f-7b09-95ab-2c8ae9fe8344" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_A14FamilyRentalMember_019e4114-566f-7b09-95ab-2c8ae9fe8344" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-71a3-876a-c4cdb5f0a842" xlink:href="mpb-20260630.xsd#mpb_HELOCAndJuniorLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7b2d-b9e3-5e1b90e29d06" xlink:to="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-71a3-876a-c4cdb5f0a842" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-73e2-a31c-e7d944c09fc1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7d1d-bbe1-192e600e3c5d" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-73e2-a31c-e7d944c09fc1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterest90DaysOrMorePastDueStillAccruing_019e4114-566f-7a04-b42a-cb25e5f6bcda" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterest90DaysOrMorePastDueStillAccruing"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7d1d-bbe1-192e600e3c5d" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterest90DaysOrMorePastDueStillAccruing_019e4114-566f-7a04-b42a-cb25e5f6bcda" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofNonaccrualLoansbyClassesoftheLoanPortfolioIncludingLoansAcquiredWithCreditDeteriorationDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansScheduleofNonaccrualLoansbyClassesoftheLoanPortfolioIncludingLoansAcquiredWithCreditDeteriorationDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofNonaccrualLoansbyClassesoftheLoanPortfolioIncludingLoansAcquiredWithCreditDeteriorationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7c31-813d-850641c922c1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-727b-962e-e9ec3c262346" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivablesPastDueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7c31-813d-850641c922c1" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-727b-962e-e9ec3c262346" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7c2f-a285-696abc311603" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-727b-962e-e9ec3c262346" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7c2f-a285-696abc311603" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c2f-a285-696abc311603_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7c2f-a285-696abc311603" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c2f-a285-696abc311603_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7ebf-a8ba-fd6beb55b057" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7c2f-a285-696abc311603" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7ebf-a8ba-fd6beb55b057" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-71a3-8461-55cba1b0bd9b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7ebf-a8ba-fd6beb55b057" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-71a3-8461-55cba1b0bd9b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-76e0-8688-585c2d9fcf93" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7ebf-a8ba-fd6beb55b057" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-76e0-8688-585c2d9fcf93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-7a28-9483-033d9c835b28" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7ebf-a8ba-fd6beb55b057" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-7a28-9483-033d9c835b28" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-79a6-9f0d-439e9ded59eb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7ebf-a8ba-fd6beb55b057" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-79a6-9f0d-439e9ded59eb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7458-b093-c5e1c417528f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-727b-962e-e9ec3c262346" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7458-b093-c5e1c417528f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7458-b093-c5e1c417528f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7458-b093-c5e1c417528f" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7458-b093-c5e1c417528f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-7458-b093-c5e1c417528f" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7828-b7a2-0d60f5651466" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7828-b7a2-0d60f5651466" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-7a8a-9292-37e177cb8479" xlink:href="mpb-20260630.xsd#mpb_CREOwnerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-7a8a-9292-37e177cb8479" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MultifamilyMember_019e4114-566f-7907-a7fd-cd39b82919ee" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MultifamilyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_srt_MultifamilyMember_019e4114-566f-7907-a7fd-cd39b82919ee" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FarmlandMember_019e4114-566f-784f-bed1-8b0dd5da1397" xlink:href="mpb-20260630.xsd#mpb_FarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_mpb_FarmlandMember_019e4114-566f-784f-bed1-8b0dd5da1397" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14Family1stLienMember_019e4114-566f-7303-bf91-61e90c63cb98" xlink:href="mpb-20260630.xsd#mpb_A14Family1stLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_mpb_A14Family1stLienMember_019e4114-566f-7303-bf91-61e90c63cb98" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019e4114-566f-7c20-a376-a71d3cb92f60" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_mpb_A14FamilyRentalMember_019e4114-566f-7c20-a376-a71d3cb92f60" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-742f-b21b-712ee3acae4c" xlink:href="mpb-20260630.xsd#mpb_HELOCAndJuniorLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7204-874a-89d423613bab" xlink:to="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-742f-b21b-712ee3acae4c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestNonaccrualWithAllowance_019e4114-566f-75f1-9367-ce3536ef0c82" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestNonaccrualWithAllowance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7c31-813d-850641c922c1" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestNonaccrualWithAllowance_019e4114-566f-75f1-9367-ce3536ef0c82" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestNonaccrualNoAllowance_019e4114-566f-7a77-a482-f262c96fabfe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestNonaccrualNoAllowance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7c31-813d-850641c922c1" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestNonaccrualNoAllowance_019e4114-566f-7a77-a482-f262c96fabfe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestNonaccrual_019e4114-566f-7956-a655-cd7d3be386f8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestNonaccrual"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentPastDueLineItems_019e4114-566f-7c31-813d-850641c922c1" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestNonaccrual_019e4114-566f-7956-a655-cd7d3be386f8" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofCreditQualityIndicatorsDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansScheduleofCreditQualityIndicatorsDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofCreditQualityIndicatorsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-72c1-ad6e-c62742725ef1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-72c1-ad6e-c62742725ef1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7d09-8906-d37822c9fba5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-72c1-ad6e-c62742725ef1" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7d09-8906-d37822c9fba5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d09-8906-d37822c9fba5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7d09-8906-d37822c9fba5" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7d09-8906-d37822c9fba5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7d09-8906-d37822c9fba5" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7dd9-8950-18e366e57ca1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7dd9-8950-18e366e57ca1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7374-8982-e01dd35320dc" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7374-8982-e01dd35320dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionLoansMember_019e4114-566f-7328-b34c-bdc85fb00186" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConstructionLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:to="loc_us-gaap_ConstructionLoansMember_019e4114-566f-7328-b34c-bdc85fb00186" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-7fd7-b230-d514010d5311" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-7fd7-b230-d514010d5311" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-7f55-9bad-00a7f6fdf803" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7693-b10d-e1b8661114e8" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-7f55-9bad-00a7f6fdf803" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-72f0-bd29-f4c68eed1366" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-72c1-ad6e-c62742725ef1" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-72f0-bd29-f4c68eed1366" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-72f0-bd29-f4c68eed1366_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-72f0-bd29-f4c68eed1366" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-72f0-bd29-f4c68eed1366_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-72f0-bd29-f4c68eed1366" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7769-b82a-09dbf02d974f" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7769-b82a-09dbf02d974f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-7b41-87dc-79c1c5141c76" xlink:href="mpb-20260630.xsd#mpb_CREOwnerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-7b41-87dc-79c1c5141c76" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MultifamilyMember_019e4114-566f-70b7-9744-bafa943b22c4" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MultifamilyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_srt_MultifamilyMember_019e4114-566f-70b7-9744-bafa943b22c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FarmlandMember_019e4114-566f-7792-b5a9-dcd381714e7d" xlink:href="mpb-20260630.xsd#mpb_FarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_FarmlandMember_019e4114-566f-7792-b5a9-dcd381714e7d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ResidentialConstructionMember_019e4114-566f-7f7c-82a8-0468c174a5c2" xlink:href="mpb-20260630.xsd#mpb_ResidentialConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_ResidentialConstructionMember_019e4114-566f-7f7c-82a8-0468c174a5c2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OtherConstructionMember_019e4114-566f-7417-8385-e9645b873f77" xlink:href="mpb-20260630.xsd#mpb_OtherConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_OtherConstructionMember_019e4114-566f-7417-8385-e9645b873f77" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14Family1stLienMember_019e4114-566f-76a0-8873-71157338f0b9" xlink:href="mpb-20260630.xsd#mpb_A14Family1stLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_A14Family1stLienMember_019e4114-566f-76a0-8873-71157338f0b9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019e4114-566f-7cf6-a630-742f23df5af8" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_A14FamilyRentalMember_019e4114-566f-7cf6-a630-742f23df5af8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-71fb-b8ee-8455e6cc0d87" xlink:href="mpb-20260630.xsd#mpb_HELOCAndJuniorLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9f-bf6c-3424df97fa42" xlink:to="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-71fb-b8ee-8455e6cc0d87" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentAxis_019e4114-566f-7cbc-93e4-1350c7fd102b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InternalCreditAssessmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-72c1-ad6e-c62742725ef1" xlink:to="loc_us-gaap_InternalCreditAssessmentAxis_019e4114-566f-7cbc-93e4-1350c7fd102b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7cbc-93e4-1350c7fd102b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_019e4114-566f-7cbc-93e4-1350c7fd102b" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7cbc-93e4-1350c7fd102b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7184-ab98-a4eba13887e9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InternalCreditAssessmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InternalCreditAssessmentAxis_019e4114-566f-7cbc-93e4-1350c7fd102b" xlink:to="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7184-ab98-a4eba13887e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PassMember_019e4114-566f-736c-a7e0-87703b59de2d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PassMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7184-ab98-a4eba13887e9" xlink:to="loc_us-gaap_PassMember_019e4114-566f-736c-a7e0-87703b59de2d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SpecialMentionMember_019e4114-566f-7e6f-aab7-582e9f87c2f2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SpecialMentionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7184-ab98-a4eba13887e9" xlink:to="loc_us-gaap_SpecialMentionMember_019e4114-566f-7e6f-aab7-582e9f87c2f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubstandardMember_019e4114-566f-70f4-b543-7ee08cdb1831" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubstandardMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InternalCreditAssessmentDomain_019e4114-566f-7184-ab98-a4eba13887e9" xlink:to="loc_us-gaap_SubstandardMember_019e4114-566f-70f4-b543-7ee08cdb1831" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_019e4114-566f-7dcd-8831-e043c57658c7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentPerformanceStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableRecordedInvestmentCreditQualityIndicatorTable_019e4114-566f-72c1-ad6e-c62742725ef1" xlink:to="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_019e4114-566f-7dcd-8831-e043c57658c7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_019e4114-566f-7dcd-8831-e043c57658c7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentPerformanceStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_019e4114-566f-7dcd-8831-e043c57658c7" xlink:to="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_019e4114-566f-7dcd-8831-e043c57658c7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_019e4114-566f-7cd1-956f-ec900c91d99d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentPerformanceStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusAxis_019e4114-566f-7dcd-8831-e043c57658c7" xlink:to="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_019e4114-566f-7cd1-956f-ec900c91d99d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformingFinancingReceivableMember_019e4114-566f-7308-bef9-17770b3a8799" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PerformingFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_019e4114-566f-7cd1-956f-ec900c91d99d" xlink:to="loc_us-gaap_PerformingFinancingReceivableMember_019e4114-566f-7308-bef9-17770b3a8799" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonperformingFinancingReceivableMember_019e4114-566f-740c-a1a6-ed2ac7106769" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NonperformingFinancingReceivableMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentPerformanceStatusDomain_019e4114-566f-7cd1-956f-ec900c91d99d" xlink:to="loc_us-gaap_NonperformingFinancingReceivableMember_019e4114-566f-740c-a1a6-ed2ac7106769" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYear_019e4114-566f-7cb1-9216-bfdc5779550e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYear_019e4114-566f-7cb1-9216-bfdc5779550e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYear_019e4114-566f-71ac-b161-4b79eccd7427" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYear_019e4114-566f-71ac-b161-4b79eccd7427" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYear_019e4114-566f-7725-abe2-513562b18db2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYear_019e4114-566f-7725-abe2-513562b18db2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYear_019e4114-566f-7b16-bea6-9f4af5af145a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYear_019e4114-566f-7b16-bea6-9f4af5af145a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYear_019e4114-566f-77d8-ad8f-178b71ac4159" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYear_019e4114-566f-77d8-ad8f-178b71ac4159" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYear_019e4114-566f-74f3-8a05-68a9639b1187" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYear_019e4114-566f-74f3-8a05-68a9639b1187" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolving_019e4114-566f-7eef-9121-d1b92777bb6c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestRevolving"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolving_019e4114-566f-7eef-9121-d1b92777bb6c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-7606-bb22-adf558a5a353" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestBeforeAllowanceForCreditLoss_019e4114-566f-7606-bb22-adf558a5a353" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearWriteoff_019e4114-566f-77dd-bd2e-f369dfc4f932" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearWriteoff_019e4114-566f-77dd-bd2e-f369dfc4f932" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearWriteoff_019e4114-566f-7b12-a51d-27f8b5734c1e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearWriteoff_019e4114-566f-7b12-a51d-27f8b5734c1e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-722a-85d9-90286f0bd146" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-722a-85d9-90286f0bd146" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-7677-9823-b1293d52b6ed" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-7677-9823-b1293d52b6ed" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-7220-940e-10245be1f144" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-7220-940e-10245be1f144" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-7d35-8dc7-47a7a92b9b46" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearWriteoff_019e4114-566f-7d35-8dc7-47a7a92b9b46" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolvingWriteoff_019e4114-566f-7863-8593-b0dfd5295eda" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestRevolvingWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestRevolvingWriteoff_019e4114-566f-7863-8593-b0dfd5295eda" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff_019e4114-566f-7104-9728-3a4663897874" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffByOriginationYearAbstract_019e4114-566f-79cd-a909-7c944bb29fb6" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff_019e4114-566f-7104-9728-3a4663897874" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearRecovery_019e4114-566f-723f-9b35-f1826b3c1d34" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearRecovery_019e4114-566f-723f-9b35-f1826b3c1d34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearRecovery_019e4114-566f-72f4-897a-c20704157c25" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearRecovery_019e4114-566f-72f4-897a-c20704157c25" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-7d44-b3d9-2d15849b6731" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-7d44-b3d9-2d15849b6731" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-7e86-b73c-b4a0a29a6650" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-7e86-b73c-b4a0a29a6650" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-776c-9f8f-c88d36a7e724" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-776c-9f8f-c88d36a7e724" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-7ca2-9c6e-04bfcc03ae2a" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearRecovery_019e4114-566f-7ca2-9c6e-04bfcc03ae2a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestRevolvingRecovery_019e4114-566f-722f-a800-f57807a44933" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestRevolvingRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestRevolvingRecovery_019e4114-566f-722f-a800-f57807a44933" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery_019e4114-566f-7d05-b623-8402518d1e77" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecoveryAbstract_019e4114-566f-77b0-9e8b-3f545ae2e60c" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery_019e4114-566f-7d05-b623-8402518d1e77" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentLineItems_019e4114-566f-7fce-befb-f67867850fae" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7df5-9810-f4c54b58c83f" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearOneOriginatedCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7df5-9810-f4c54b58c83f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-77c4-9e89-dde2b765e669" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearTwoOriginatedFiscalYearBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-77c4-9e89-dde2b765e669" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7d5d-a4bc-845087ee89dc" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearThreeOriginatedTwoYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7d5d-a4bc-845087ee89dc" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7f67-80f3-125e130ef12d" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFourOriginatedThreeYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7f67-80f3-125e130ef12d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7b30-b1b0-4e516af7162d" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestYearFiveOriginatedFourYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7b30-b1b0-4e516af7162d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7637-9a5b-6d2915d0ad8b" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestOriginatedMoreThanFiveYearsBeforeCurrentFiscalYearNetWriteoffRecovery_019e4114-566f-7637-9a5b-6d2915d0ad8b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableExcludingAccruedInterestRevolvingNetWriteoffRecovery_019e4114-566f-784e-9117-8341c32de01e" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableExcludingAccruedInterestRevolvingNetWriteoffRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_mpb_FinancingReceivableExcludingAccruedInterestRevolvingNetWriteoffRecovery_019e4114-566f-784e-9117-8341c32de01e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecovery_019e4114-566f-7c4b-9d50-2f4f1cc69a5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecoveryAbstract_019e4114-566f-7221-967c-fd40149d5a11" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoffAfterRecovery_019e4114-566f-7c4b-9d50-2f4f1cc69a5c" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofACLLoansbyPortfolioSegmentDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansScheduleofACLLoansbyPortfolioSegmentDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofACLLoansbyPortfolioSegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-77d7-ace9-a713644afc09" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-73e9-a420-d9b77b9a0bf9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-77d7-ace9-a713644afc09" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-73e9-a420-d9b77b9a0bf9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7369-acb5-7cddb8442998" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-73e9-a420-d9b77b9a0bf9" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7369-acb5-7cddb8442998" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7369-acb5-7cddb8442998_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7369-acb5-7cddb8442998" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7369-acb5-7cddb8442998_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-7369-acb5-7cddb8442998" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7cd3-8d0a-08c9246fe17e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019e4114-566f-7cd3-8d0a-08c9246fe17e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7b3f-9dee-3178bf373588" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7b3f-9dee-3178bf373588" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConstructionLoansMember_019e4114-566f-7205-8fa4-ab4b3c4ffd16" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConstructionLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:to="loc_us-gaap_ConstructionLoansMember_019e4114-566f-7205-8fa4-ab4b3c4ffd16" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-779f-af6f-f2fb55b1a58e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_019e4114-566f-779f-af6f-f2fb55b1a58e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-7797-99c8-5a93549cec15" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-79d5-8661-62a6db70f86e" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_019e4114-566f-7797-99c8-5a93549cec15" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-727e-bbdc-917521660ade" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-73e9-a420-d9b77b9a0bf9" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-727e-bbdc-917521660ade" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-727e-bbdc-917521660ade_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-727e-bbdc-917521660ade" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-727e-bbdc-917521660ade_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-566f-727e-bbdc-917521660ade" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7634-b6ae-a76e819ae977" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_CRENonownerOccupiedMember_019e4114-566f-7634-b6ae-a76e819ae977" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-79df-8042-83cbf285c3e2" xlink:href="mpb-20260630.xsd#mpb_CREOwnerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_CREOwnerOccupiedMember_019e4114-566f-79df-8042-83cbf285c3e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MultifamilyMember_019e4114-566f-78df-bc9e-1ff840f49f87" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MultifamilyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_srt_MultifamilyMember_019e4114-566f-78df-bc9e-1ff840f49f87" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FarmlandMember_019e4114-566f-7f88-9d1f-8681b15ba7c4" xlink:href="mpb-20260630.xsd#mpb_FarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_FarmlandMember_019e4114-566f-7f88-9d1f-8681b15ba7c4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ResidentialConstructionMember_019e4114-566f-704f-a8c8-aa393fddc481" xlink:href="mpb-20260630.xsd#mpb_ResidentialConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_ResidentialConstructionMember_019e4114-566f-704f-a8c8-aa393fddc481" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OtherConstructionMember_019e4114-566f-7b22-a8b7-a863b54c912e" xlink:href="mpb-20260630.xsd#mpb_OtherConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_OtherConstructionMember_019e4114-566f-7b22-a8b7-a863b54c912e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14Family1stLienMember_019e4114-566f-7ff2-958b-64612d949de9" xlink:href="mpb-20260630.xsd#mpb_A14Family1stLienMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_A14Family1stLienMember_019e4114-566f-7ff2-958b-64612d949de9" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019e4114-566f-7fd2-a311-d396a5cca267" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_A14FamilyRentalMember_019e4114-566f-7fd2-a311-d396a5cca267" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-7eaf-b28b-eafadc3ce79f" xlink:href="mpb-20260630.xsd#mpb_HELOCAndJuniorLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-566f-7e9c-b9f0-ced00b9f3458" xlink:to="loc_mpb_HELOCAndJuniorLiensMember_019e4114-566f-7eaf-b28b-eafadc3ce79f" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7795-ab03-16ead2f8a91a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivableAllowanceForCreditLossesTable_019e4114-566f-73e9-a420-d9b77b9a0bf9" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7795-ab03-16ead2f8a91a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7795-ab03-16ead2f8a91a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7795-ab03-16ead2f8a91a" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7795-ab03-16ead2f8a91a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7404-8838-cee8f77a1992" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-566f-7795-ab03-16ead2f8a91a" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7404-8838-cee8f77a1992" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A1stColonialAcquisitionMember_019eb73f-6694-76fb-ba3e-68b4315ce952" xlink:href="mpb-20260630.xsd#mpb_A1stColonialAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7404-8838-cee8f77a1992" xlink:to="loc_mpb_A1stColonialAcquisitionMember_019eb73f-6694-76fb-ba3e-68b4315ce952" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_WilliamPennAcquisitionMember_019e4114-566f-76c0-8971-ea9d0707fda9" xlink:href="mpb-20260630.xsd#mpb_WilliamPennAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-566f-7404-8838-cee8f77a1992" xlink:to="loc_mpb_WilliamPennAcquisitionMember_019e4114-566f-76c0-8971-ea9d0707fda9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesLineItems_019e4114-566f-77d7-ace9-a713644afc09" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_019e4114-566f-7604-835f-b44d2736fc6c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_019e4114-566f-7604-835f-b44d2736fc6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestPurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate_019e4114-566f-7c0b-8480-ffd6569888b9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestPurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestPurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate_019e4114-566f-7c0b-8480-ffd6569888b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossPurchasedSeasonedLoan_019eb740-692a-7bfa-a9b2-badf7b4de045" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossPurchasedSeasonedLoan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossPurchasedSeasonedLoan_019eb740-692a-7bfa-a9b2-badf7b4de045" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff_019e4114-566f-7bdb-a071-ade648791c61" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossWriteoff_019e4114-566f-7bdb-a071-ade648791c61" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery_019e4114-566f-7329-a65a-af439a35f6d9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestAllowanceForCreditLossRecovery_019e4114-566f-7329-a65a-af439a35f6d9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossWriteoffAfterRecovery_019e4114-566f-7e85-a96a-88e61c1e2a2a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossWriteoffAfterRecovery"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableAllowanceForCreditLossWriteoffAfterRecovery_019e4114-566f-7e85-a96a-88e61c1e2a2a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestCreditLossExpenseReversal_019e4114-566f-7422-bee7-48e2d13172d1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestCreditLossExpenseReversal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestCreditLossExpenseReversal_019e4114-566f-7422-bee7-48e2d13172d1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest_019e4114-566f-7a35-b51e-fa5de5e89949" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossExcludingAccruedInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate_019e736e-ce56-782f-8a91-37bda2697710" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_us-gaap_FinancingReceivablePurchasedWithCreditDeteriorationAllowanceForCreditLossAtAcquisitionDate_019e736e-ce56-782f-8a91-37bda2697710" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableAllowanceForCreditLossPurchasedSeasonedLoan_019e7379-85c3-77a6-abd1-910f15258214" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableAllowanceForCreditLossPurchasedSeasonedLoan"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivablePurchasedWithCreditDeteriorationProvisionExpense_019e4114-566f-7442-ae9e-8366c8071b5d" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivablePurchasedWithCreditDeteriorationProvisionExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableAllowanceForCreditLossesRollForward_019e4114-566f-73e8-b64f-30fabd9cc0af" xlink:to="loc_mpb_FinancingReceivablePurchasedWithCreditDeteriorationProvisionExpense_019e4114-566f-7442-ae9e-8366c8071b5d" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofTroubledDebtRestructuringsDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#LoansandAllowanceforCreditLossesLoansScheduleofTroubledDebtRestructuringsDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/LoansandAllowanceforCreditLossesLoansScheduleofTroubledDebtRestructuringsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationsLineItems_019e4114-566f-7f1d-8e51-6fabd6f2a2a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableModificationsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-72a8-a555-b1558d836f6a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFinancingReceivablesPastDueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_019e4114-566f-7f1d-8e51-6fabd6f2a2a9" xlink:to="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-72a8-a555-b1558d836f6a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-75b2-b86e-25c04afa8ffd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-72a8-a555-b1558d836f6a" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-75b2-b86e-25c04afa8ffd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-75b2-b86e-25c04afa8ffd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-75b2-b86e-25c04afa8ffd" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-75b2-b86e-25c04afa8ffd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c38-b4cb-c6d09f73d92a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivablePortfolioSegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentAxis_019e4114-566f-75b2-b86e-25c04afa8ffd" xlink:to="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c38-b4cb-c6d09f73d92a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019f835d-6c31-70b8-b061-de691a45316d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialRealEstatePortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c38-b4cb-c6d09f73d92a" xlink:to="loc_us-gaap_CommercialRealEstatePortfolioSegmentMember_019f835d-6c31-70b8-b061-de691a45316d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7ce5-b944-2db14529b5af" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c38-b4cb-c6d09f73d92a" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-566f-7ce5-b944-2db14529b5af" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResidentialPortfolioSegmentMember_019f835f-2bce-7fb2-8327-77dfd16e73dd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResidentialPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c38-b4cb-c6d09f73d92a" xlink:to="loc_us-gaap_ResidentialPortfolioSegmentMember_019f835f-2bce-7fb2-8327-77dfd16e73dd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConsumerPortfolioSegmentMember_019f8360-11fc-7d81-ad7e-d3caf100d236" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConsumerPortfolioSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivablePortfolioSegmentDomain_019e4114-566f-7c38-b4cb-c6d09f73d92a" xlink:to="loc_us-gaap_ConsumerPortfolioSegmentMember_019f8360-11fc-7d81-ad7e-d3caf100d236" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoanRestructuringModificationAxis_019e4114-566f-7d09-8f02-0cf09efb0ee1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoanRestructuringModificationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-72a8-a555-b1558d836f6a" xlink:to="loc_us-gaap_LoanRestructuringModificationAxis_019e4114-566f-7d09-8f02-0cf09efb0ee1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-7d09-8f02-0cf09efb0ee1_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoanRestructuringModificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LoanRestructuringModificationAxis_019e4114-566f-7d09-8f02-0cf09efb0ee1" xlink:to="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-7d09-8f02-0cf09efb0ee1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-720e-9328-3def39f87ec0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoanRestructuringModificationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LoanRestructuringModificationAxis_019e4114-566f-7d09-8f02-0cf09efb0ee1" xlink:to="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-720e-9328-3def39f87ec0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractualInterestRateReductionMember_019e4114-566f-7b09-9905-4772db97328c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractualInterestRateReductionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-720e-9328-3def39f87ec0" xlink:to="loc_us-gaap_ContractualInterestRateReductionMember_019e4114-566f-7b09-9905-4772db97328c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ExtendedMaturityMember_019e4114-566f-7cb0-b559-f939aa959701" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExtendedMaturityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-720e-9328-3def39f87ec0" xlink:to="loc_us-gaap_ExtendedMaturityMember_019e4114-566f-7cb0-b559-f939aa959701" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ExtendedMaturityAndInterestRateReductionMember_019e4114-566f-7b5d-a352-81f2b6e36cea" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExtendedMaturityAndInterestRateReductionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LoanRestructuringModificationDomain_019e4114-566f-720e-9328-3def39f87ec0" xlink:to="loc_us-gaap_ExtendedMaturityAndInterestRateReductionMember_019e4114-566f-7b5d-a352-81f2b6e36cea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019f835d-6c31-7c96-af54-e024879f64fe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFinancingReceivablesPastDueTable_019e4114-566f-72a8-a555-b1558d836f6a" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019f835d-6c31-7c96-af54-e024879f64fe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019f835d-6c31-7c96-af54-e024879f64fe_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019f835d-6c31-7c96-af54-e024879f64fe" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019f835d-6c31-7c96-af54-e024879f64fe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019f835d-6c31-7a78-ab46-09ee8df18552" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019f835d-6c31-7c96-af54-e024879f64fe" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019f835d-6c31-7a78-ab46-09ee8df18552" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019f835d-6c31-70b0-b922-e726d58023f2" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019f835d-6c31-7a78-ab46-09ee8df18552" xlink:to="loc_mpb_CRENonownerOccupiedMember_019f835d-6c31-70b0-b922-e726d58023f2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019f835f-8ab0-7283-893f-114550576af5" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019f835d-6c31-7a78-ab46-09ee8df18552" xlink:to="loc_mpb_A14FamilyRentalMember_019f835f-8ab0-7283-893f-114550576af5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPeriod_019e4114-566f-7027-a618-f8110cfd8027" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_019e4114-566f-7f1d-8e51-6fabd6f2a2a9" xlink:to="loc_us-gaap_FinancingReceivableExcludingAccruedInterestModifiedPeriod_019e4114-566f-7027-a618-f8110cfd8027" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableModificationToTotalFinancingReceivablesExcludingAccruedInterestPercent_019e4114-566f-71f0-ba07-3ba05c8540b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableModificationToTotalFinancingReceivablesExcludingAccruedInterestPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableModificationsLineItems_019e4114-566f-7f1d-8e51-6fabd6f2a2a9" xlink:to="loc_us-gaap_FinancingReceivableModificationToTotalFinancingReceivablesExcludingAccruedInterestPercent_019e4114-566f-71f0-ba07-3ba05c8540b8" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/DepositsScheduleofTimeDepositsByMaturityDateDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#DepositsScheduleofTimeDepositsByMaturityDateDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/DepositsScheduleofTimeDepositsByMaturityDateDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepositLiabilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositsTable_019e4114-566f-700d-b24f-5f82626b38d2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDepositsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDepositsTable_019e4114-566f-700d-b24f-5f82626b38d2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TimeDepositDurationAxis_019e4114-566f-71c4-8c12-29e19942d93f" xlink:href="mpb-20260630.xsd#mpb_TimeDepositDurationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_TimeDepositsTable_019e4114-566f-700d-b24f-5f82626b38d2" xlink:to="loc_mpb_TimeDepositDurationAxis_019e4114-566f-71c4-8c12-29e19942d93f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TimeDepositDurationDomain_019e4114-566f-71c4-8c12-29e19942d93f_default" xlink:href="mpb-20260630.xsd#mpb_TimeDepositDurationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_mpb_TimeDepositDurationAxis_019e4114-566f-71c4-8c12-29e19942d93f" xlink:to="loc_mpb_TimeDepositDurationDomain_019e4114-566f-71c4-8c12-29e19942d93f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TimeDepositDurationDomain_019e4114-566f-7990-9115-edc88803bc74" xlink:href="mpb-20260630.xsd#mpb_TimeDepositDurationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_mpb_TimeDepositDurationAxis_019e4114-566f-71c4-8c12-29e19942d93f" xlink:to="loc_mpb_TimeDepositDurationDomain_019e4114-566f-7990-9115-edc88803bc74" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TimeDepositsLessThan250000Member_019e4114-566f-7ed9-95f3-c64af27315b5" xlink:href="mpb-20260630.xsd#mpb_TimeDepositsLessThan250000Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_TimeDepositDurationDomain_019e4114-566f-7990-9115-edc88803bc74" xlink:to="loc_mpb_TimeDepositsLessThan250000Member_019e4114-566f-7ed9-95f3-c64af27315b5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TimeDeposits250000OrMoreMember_019e4114-566f-763a-acff-3fba090e92a0" xlink:href="mpb-20260630.xsd#mpb_TimeDeposits250000OrMoreMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_TimeDepositDurationDomain_019e4114-566f-7990-9115-edc88803bc74" xlink:to="loc_mpb_TimeDeposits250000OrMoreMember_019e4114-566f-763a-acff-3fba090e92a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositMaturitiesRemainderOfFiscalYear_019e4114-566f-7ad8-9710-0d6fa5b0c310" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDepositMaturitiesRemainderOfFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDepositMaturitiesRemainderOfFiscalYear_019e4114-566f-7ad8-9710-0d6fa5b0c310" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositMaturitiesYearOne_019e4114-566f-78a6-b527-058b05de680d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDepositMaturitiesYearOne"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDepositMaturitiesYearOne_019e4114-566f-78a6-b527-058b05de680d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositMaturitiesYearTwo_019e4114-566f-7b06-a662-607520732568" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDepositMaturitiesYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDepositMaturitiesYearTwo_019e4114-566f-7b06-a662-607520732568" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositMaturitiesYearThree_019e4114-566f-7d88-b214-b1bb3c7c7aec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDepositMaturitiesYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDepositMaturitiesYearThree_019e4114-566f-7d88-b214-b1bb3c7c7aec" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDepositMaturitiesYearFour_019e4114-566f-7ecb-b9fb-1e6f19dc3422" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDepositMaturitiesYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDepositMaturitiesYearFour_019e4114-566f-7ecb-b9fb-1e6f19dc3422" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TimeDepositMaturitiesAfterYearFour_019e4114-566f-7ba8-b3cd-bc65e1393ac5" xlink:href="mpb-20260630.xsd#mpb_TimeDepositMaturitiesAfterYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_mpb_TimeDepositMaturitiesAfterYearFour_019e4114-566f-7ba8-b3cd-bc65e1393ac5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TimeDeposits_019e4114-566f-7d9c-b9c3-c08e311c0ef2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TimeDeposits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DepositLiabilityLineItems_019e4114-566f-77b1-afa6-68fd855b6698" xlink:to="loc_us-gaap_TimeDeposits_019e4114-566f-7d9c-b9c3-c08e311c0ef2" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/DerivativeFinancialInstrumentsScheduleofLoanLevelSwapsDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#DerivativeFinancialInstrumentsScheduleofLoanLevelSwapsDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/DerivativeFinancialInstrumentsScheduleofLoanLevelSwapsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_019e4114-566f-7155-a794-ae35a495c2d7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeTable_019e4114-566f-7155-a794-ae35a495c2d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7e49-80fa-bd0879abea0d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_019e4114-566f-7155-a794-ae35a495c2d7" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7e49-80fa-bd0879abea0d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-7e49-80fa-bd0879abea0d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7e49-80fa-bd0879abea0d" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-7e49-80fa-bd0879abea0d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-731c-87ee-0c576f57b988" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7e49-80fa-bd0879abea0d" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-731c-87ee-0c576f57b988" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_InterestRateSwapWithCustomersMember_019e4114-566f-7bbe-990b-148b272f5471" xlink:href="mpb-20260630.xsd#mpb_InterestRateSwapWithCustomersMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-731c-87ee-0c576f57b988" xlink:to="loc_mpb_InterestRateSwapWithCustomersMember_019e4114-566f-7bbe-990b-148b272f5471" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_InterestRateSwapWithCounterpartiesMember_019e4114-566f-7ccf-9ea3-05573610efbb" xlink:href="mpb-20260630.xsd#mpb_InterestRateSwapWithCounterpartiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-731c-87ee-0c576f57b988" xlink:to="loc_mpb_InterestRateSwapWithCounterpartiesMember_019e4114-566f-7ccf-9ea3-05573610efbb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_InterestRateSwapsUsedInCashFlowHedgesMember_019e4114-566f-7e0d-9dd0-385ae3b45620" xlink:href="mpb-20260630.xsd#mpb_InterestRateSwapsUsedInCashFlowHedgesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-731c-87ee-0c576f57b988" xlink:to="loc_mpb_InterestRateSwapsUsedInCashFlowHedgesMember_019e4114-566f-7e0d-9dd0-385ae3b45620" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateFloorMember_019fc7ae-8003-7d22-9170-095d4932e427" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestRateFloorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-731c-87ee-0c576f57b988" xlink:to="loc_us-gaap_InterestRateFloorMember_019fc7ae-8003-7d22-9170-095d4932e427" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7db6-85ee-dbb176d65010" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_019e4114-566f-7155-a794-ae35a495c2d7" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7db6-85ee-dbb176d65010" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7db6-85ee-dbb176d65010_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7db6-85ee-dbb176d65010" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-7db6-85ee-dbb176d65010_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-75f1-b9b6-b4227cb17cc2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-566f-7db6-85ee-dbb176d65010" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-75f1-b9b6-b4227cb17cc2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialLoanMember_019e4114-566f-72ee-b48a-856e21b5434c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommercialLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-75f1-b9b6-b4227cb17cc2" xlink:to="loc_us-gaap_CommercialLoanMember_019e4114-566f-72ee-b48a-856e21b5434c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashFlowHedgingMember_019e4114-566f-7368-a730-231ce3d2dbcc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashFlowHedgingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-566f-75f1-b9b6-b4227cb17cc2" xlink:to="loc_us-gaap_CashFlowHedgingMember_019e4114-566f-7368-a730-231ce3d2dbcc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeNotionalAmount_019e4114-566f-7a81-b072-fb7ad01e988b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeNotionalAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeNotionalAmount_019e4114-566f-7a81-b072-fb7ad01e988b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAverageRemainingMaturity1_019e4114-566f-730c-8595-11564f91cdc1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeAverageRemainingMaturity1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeAverageRemainingMaturity1_019e4114-566f-730c-8595-11564f91cdc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFloorInterestRate_019fc7af-7f85-7c71-9ea9-667e7618b18b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeFloorInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeFloorInterestRate_019fc7af-7f85-7c71-9ea9-667e7618b18b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAverageFixedInterestRate_019e4114-566f-7529-92bf-16ea5619f9f7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeAverageFixedInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeAverageFixedInterestRate_019e4114-566f-7529-92bf-16ea5619f9f7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAverageVariableInterestRate_019e4114-566f-7a25-bfca-5d295bb0c68d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeAverageVariableInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeAverageVariableInterestRate_019e4114-566f-7a25-bfca-5d295bb0c68d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeFairValueOfDerivativeNet_019e4114-566f-79e2-a816-805c404ac25f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeFairValueOfDerivativeNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7cf6-bb1b-5dc859543b6c" xlink:to="loc_us-gaap_DerivativeFairValueOfDerivativeNet_019e4114-566f-79e2-a816-805c404ac25f" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/DerivativeFinancialInstrumentsNarrativeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#DerivativeFinancialInstrumentsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/DerivativeFinancialInstrumentsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLineItems_019e4114-566f-7d56-9bdb-c12972ff7471" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeTable_019e4114-566f-7c59-a19d-528528640c70" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7d56-9bdb-c12972ff7471" xlink:to="loc_us-gaap_DerivativeTable_019e4114-566f-7c59-a19d-528528640c70" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7aa7-9c17-715c17bf694e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeInstrumentRiskAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeTable_019e4114-566f-7c59-a19d-528528640c70" xlink:to="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7aa7-9c17-715c17bf694e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-7aa7-9c17-715c17bf694e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7aa7-9c17-715c17bf694e" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-7aa7-9c17-715c17bf694e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-721d-96a6-8074e3bd683c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DerivativeInstrumentRiskAxis_019e4114-566f-7aa7-9c17-715c17bf694e" xlink:to="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-721d-96a6-8074e3bd683c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateContractMember_019e4114-566f-791f-abf4-cf464b1a8eaa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestRateContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeContractTypeDomain_019e4114-566f-721d-96a6-8074e3bd683c" xlink:to="loc_us-gaap_InterestRateContractMember_019e4114-566f-791f-abf4-cf464b1a8eaa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashFlowHedgeGainLossToBeReclassifiedWithinTwelveMonths_019e4114-566f-7ec4-9b81-bac1410accce" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashFlowHedgeGainLossToBeReclassifiedWithinTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeLineItems_019e4114-566f-7d56-9bdb-c12972ff7471" xlink:to="loc_us-gaap_CashFlowHedgeGainLossToBeReclassifiedWithinTwelveMonths_019e4114-566f-7ec4-9b81-bac1410accce" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/AccumulatedOtherComprehensiveLossIncomeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#AccumulatedOtherComprehensiveLossIncomeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/AccumulatedOtherComprehensiveLossIncomeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_019e4114-573d-7868-bebc-953d2bbb0068" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_019e4114-573d-7367-bb5d-36fc423b6159" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_019e4114-573d-7868-bebc-953d2bbb0068" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_019e4114-573d-7367-bb5d-36fc423b6159" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019e4114-573d-71f9-9132-1cd52e45dbbe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossTable_019e4114-573d-7367-bb5d-36fc423b6159" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019e4114-573d-71f9-9132-1cd52e45dbbe" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019e4114-573d-71f9-9132-1cd52e45dbbe_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019e4114-573d-71f9-9132-1cd52e45dbbe" xlink:to="loc_us-gaap_EquityComponentDomain_019e4114-573d-71f9-9132-1cd52e45dbbe_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019e4114-573d-77e5-b061-9ff789853f5f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019e4114-573d-71f9-9132-1cd52e45dbbe" xlink:to="loc_us-gaap_EquityComponentDomain_019e4114-573d-77e5-b061-9ff789853f5f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_019e4114-573d-76ba-bb06-8a6abd97135f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-573d-77e5-b061-9ff789853f5f" xlink:to="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_019e4114-573d-76ba-bb06-8a6abd97135f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_019e4114-573d-7c27-80ec-f630bb508e1b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-573d-77e5-b061-9ff789853f5f" xlink:to="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_019e4114-573d-7c27-80ec-f630bb508e1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_019e4114-573d-7219-b02c-5d4a86fccb1f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-573d-77e5-b061-9ff789853f5f" xlink:to="loc_us-gaap_AccumulatedDefinedBenefitPlansAdjustmentMember_019e4114-573d-7219-b02c-5d4a86fccb1f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019e4114-573d-727e-94b7-851f8821f547" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019e4114-573d-77e5-b061-9ff789853f5f" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019e4114-573d-727e-94b7-851f8821f547" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019e4114-573d-7ee5-a7cf-a6f5e575d60e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_019e4114-573d-7868-bebc-953d2bbb0068" xlink:to="loc_us-gaap_StockholdersEquity_019e4114-573d-7ee5-a7cf-a6f5e575d60e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent_019e4114-573d-7113-bcc6-02e2cf9533cb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_019e4114-573d-7868-bebc-953d2bbb0068" xlink:to="loc_us-gaap_OciBeforeReclassificationsNetOfTaxAttributableToParent_019e4114-573d-7113-bcc6-02e2cf9533cb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_019e4114-573d-7ebc-9a8d-601fbbc7775b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossLineItems_019e4114-573d-7868-bebc-953d2bbb0068" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodNetOfTaxAttributableToParent_019e4114-573d-7ebc-9a8d-601fbbc7775b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019e4114-573d-769b-aaf0-71d2c2b8c7b2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/FairValueMeasurementScheduleofAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#FairValueMeasurementScheduleofAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/FairValueMeasurementScheduleofAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-765f-b563-9b736de0b5ff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-765f-b563-9b736de0b5ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-76f0-b410-401428beea2f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-765f-b563-9b736de0b5ff" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-76f0-b410-401428beea2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-76f0-b410-401428beea2f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-76f0-b410-401428beea2f" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-76f0-b410-401428beea2f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c65-a3a8-3d9cab4bd214" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-76f0-b410-401428beea2f" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c65-a3a8-3d9cab4bd214" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019e4114-573d-7040-8d26-d3ab393a204e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c65-a3a8-3d9cab4bd214" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019e4114-573d-7040-8d26-d3ab393a204e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019e4114-573d-753e-8ed1-ee0a7ff719ea" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c65-a3a8-3d9cab4bd214" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019e4114-573d-753e-8ed1-ee0a7ff719ea" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019e4114-573d-7413-bf56-60444e299db6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c65-a3a8-3d9cab4bd214" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019e4114-573d-7413-bf56-60444e299db6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_019e4114-573d-773e-a23b-8aa417adef39" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-765f-b563-9b736de0b5ff" xlink:to="loc_us-gaap_FinancialInstrumentAxis_019e4114-573d-773e-a23b-8aa417adef39" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-773e-a23b-8aa417adef39_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-573d-773e-a23b-8aa417adef39" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-773e-a23b-8aa417adef39_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-77b4-8ab0-808297e7f383" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_019e4114-573d-773e-a23b-8aa417adef39" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-77b4-8ab0-808297e7f383" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_USTreasuryAndUSGovernmentAgenciesMember_019e4114-573d-737b-affd-0b9bdca7998d" xlink:href="mpb-20260630.xsd#mpb_USTreasuryAndUSGovernmentAgenciesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-77b4-8ab0-808297e7f383" xlink:to="loc_mpb_USTreasuryAndUSGovernmentAgenciesMember_019e4114-573d-737b-affd-0b9bdca7998d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-573d-7730-989b-0afb2b366275" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-77b4-8ab0-808297e7f383" xlink:to="loc_us-gaap_MortgageBackedSecuritiesIssuedByUSGovernmentSponsoredEnterprisesMember_019e4114-573d-7730-989b-0afb2b366275" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-573d-7ab7-a2ae-70d7fcb26211" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_USStatesAndPoliticalSubdivisionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-77b4-8ab0-808297e7f383" xlink:to="loc_us-gaap_USStatesAndPoliticalSubdivisionsMember_019e4114-573d-7ab7-a2ae-70d7fcb26211" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-573d-7f89-b798-67115882fc34" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CorporateDebtSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_019e4114-573d-77b4-8ab0-808297e7f383" xlink:to="loc_us-gaap_CorporateDebtSecuritiesMember_019e4114-573d-7f89-b798-67115882fc34" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019eb6bd-f78d-7823-be5c-6f5602e0e275" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-765f-b563-9b736de0b5ff" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019eb6bd-f78d-7823-be5c-6f5602e0e275" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_019eb6bd-f78d-7823-be5c-6f5602e0e275_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019eb6bd-f78d-7823-be5c-6f5602e0e275" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_019eb6bd-f78d-7823-be5c-6f5602e0e275_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_019eb6bd-f78d-74ed-aba6-8db4e3ff4e2a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019eb6bd-f78d-7823-be5c-6f5602e0e275" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_019eb6bd-f78d-74ed-aba6-8db4e3ff4e2a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_019eb6be-250d-738f-a1df-d89c938263b4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_019eb6bd-f78d-74ed-aba6-8db4e3ff4e2a" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_019eb6be-250d-738f-a1df-d89c938263b4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-573d-7534-b80b-ea7cd33466d6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-573d-7534-b80b-ea7cd33466d6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_019f043a-2256-72f0-9951-588be9d5831a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:to="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_019f043a-2256-72f0-9951-588be9d5831a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansHeldForSaleFairValueDisclosure_019e4114-573d-708d-8465-325c2766c036" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoansHeldForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:to="loc_us-gaap_LoansHeldForSaleFairValueDisclosure_019e4114-573d-708d-8465-325c2766c036" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-7f25-9f4b-24fb28f42182" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-7f25-9f4b-24fb28f42182" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_019e4114-573d-70ea-b91e-0aabf6501c5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-7f25-9f4b-24fb28f42182" xlink:to="loc_us-gaap_DerivativeAssets_019e4114-573d-70ea-b91e-0aabf6501c5c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7246-bc92-ba35b7a754d3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-733b-9831-3424a9715ff8" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7246-bc92-ba35b7a754d3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_019e4114-573d-7eed-96ec-d1430432ed52" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7246-bc92-ba35b7a754d3" xlink:to="loc_us-gaap_DerivativeLiabilities_019e4114-573d-7eed-96ec-d1430432ed52" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/FairValueMeasurementScheduleofFairValueMeasurementsNonrecurringDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#FairValueMeasurementScheduleofFairValueMeasurementsNonrecurringDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/FairValueMeasurementScheduleofFairValueMeasurementsNonrecurringDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-744d-84d9-46bebefc9dd7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-744d-84d9-46bebefc9dd7" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByAssetClassAxis_019e4114-573d-7036-b2f4-f6d7638ab0ef" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByAssetClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:to="loc_us-gaap_FairValueByAssetClassAxis_019e4114-573d-7036-b2f4-f6d7638ab0ef" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019e4114-573d-7036-b2f4-f6d7638ab0ef_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_019e4114-573d-7036-b2f4-f6d7638ab0ef" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019e4114-573d-7036-b2f4-f6d7638ab0ef_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019e4114-573d-79b4-a94f-b4e6b9e461a7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_019e4114-573d-7036-b2f4-f6d7638ab0ef" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019e4114-573d-79b4-a94f-b4e6b9e461a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_IndividuallyEvaluatedLoansNetOfAllowanceForCreditLossMember_019e4114-573d-7c60-88bc-3989406eca1a" xlink:href="mpb-20260630.xsd#mpb_IndividuallyEvaluatedLoansNetOfAllowanceForCreditLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019e4114-573d-79b4-a94f-b4e6b9e461a7" xlink:to="loc_mpb_IndividuallyEvaluatedLoansNetOfAllowanceForCreditLossMember_019e4114-573d-7c60-88bc-3989406eca1a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ForeclosedAssetsHeldForSaleMember_019e4114-573d-7448-9904-71293ddba4ec" xlink:href="mpb-20260630.xsd#mpb_ForeclosedAssetsHeldForSaleMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019e4114-573d-79b4-a94f-b4e6b9e461a7" xlink:to="loc_mpb_ForeclosedAssetsHeldForSaleMember_019e4114-573d-7448-9904-71293ddba4ec" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7bf6-b3b8-cab90ec1e27e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7bf6-b3b8-cab90ec1e27e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7bf6-b3b8-cab90ec1e27e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7bf6-b3b8-cab90ec1e27e" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7bf6-b3b8-cab90ec1e27e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7d4a-8fa9-0387250c41d6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7bf6-b3b8-cab90ec1e27e" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7d4a-8fa9-0387250c41d6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019e4114-573d-730c-9c7a-cc496729cc3c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7d4a-8fa9-0387250c41d6" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019e4114-573d-730c-9c7a-cc496729cc3c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019e4114-573d-73af-ab7d-7fcf1bc8d185" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7d4a-8fa9-0387250c41d6" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019e4114-573d-73af-ab7d-7fcf1bc8d185" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019e4114-573d-7795-a223-4651478986ab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7d4a-8fa9-0387250c41d6" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019e4114-573d-7795-a223-4651478986ab" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019e4114-573d-7655-92ed-6d7dea70c966" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019e4114-573d-7655-92ed-6d7dea70c966" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_019e4114-573d-7655-92ed-6d7dea70c966_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019e4114-573d-7655-92ed-6d7dea70c966" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_019e4114-573d-7655-92ed-6d7dea70c966_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_019e4114-573d-747e-9264-52a1a3f54441" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_019e4114-573d-7655-92ed-6d7dea70c966" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_019e4114-573d-747e-9264-52a1a3f54441" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsNonrecurringMember_019e4114-573d-74bc-9c60-cdcc6e69b292" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsNonrecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_019e4114-573d-747e-9264-52a1a3f54441" xlink:to="loc_us-gaap_FairValueMeasurementsNonrecurringMember_019e4114-573d-74bc-9c60-cdcc6e69b292" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019e4114-573d-7c83-a0e6-e02c792c09f8" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:to="loc_srt_RangeAxis_019e4114-573d-7c83-a0e6-e02c792c09f8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019e4114-573d-7c83-a0e6-e02c792c09f8_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019e4114-573d-7c83-a0e6-e02c792c09f8" xlink:to="loc_srt_RangeMember_019e4114-573d-7c83-a0e6-e02c792c09f8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019e4114-573d-7293-af57-0fa63f2f3647" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019e4114-573d-7c83-a0e6-e02c792c09f8" xlink:to="loc_srt_RangeMember_019e4114-573d-7293-af57-0fa63f2f3647" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019e4114-573d-7f2e-86d2-04e4d6c03c5e" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e4114-573d-7293-af57-0fa63f2f3647" xlink:to="loc_srt_MinimumMember_019e4114-573d-7f2e-86d2-04e4d6c03c5e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019e4114-573d-7f49-8cf9-983fe9dbef26" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e4114-573d-7293-af57-0fa63f2f3647" xlink:to="loc_srt_MaximumMember_019e4114-573d-7f49-8cf9-983fe9dbef26" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_WeightedAverageMember_019e4114-573d-70f4-908a-9143583d90ed" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_WeightedAverageMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e4114-573d-7293-af57-0fa63f2f3647" xlink:to="loc_srt_WeightedAverageMember_019e4114-573d-70f4-908a-9143583d90ed" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_019e4114-573d-7494-a327-7d1bc0989fe9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_019e4114-573d-7494-a327-7d1bc0989fe9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019e4114-573d-7494-a327-7d1bc0989fe9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019e4114-573d-7494-a327-7d1bc0989fe9" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019e4114-573d-7494-a327-7d1bc0989fe9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019e4114-573d-7643-800c-50ee6b0750b0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019e4114-573d-7494-a327-7d1bc0989fe9" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019e4114-573d-7643-800c-50ee6b0750b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputAppraisedValueMember_019e4114-573d-7ea0-901b-922cd0c9d5e1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputAppraisedValueMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019e4114-573d-7643-800c-50ee6b0750b0" xlink:to="loc_us-gaap_MeasurementInputAppraisedValueMember_019e4114-573d-7ea0-901b-922cd0c9d5e1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueAxis_019e4114-573d-77d1-9a06-000463c1d215" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ValuationTechniqueAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019e4114-573d-73f8-b375-e440368399f9" xlink:to="loc_us-gaap_ValuationTechniqueAxis_019e4114-573d-77d1-9a06-000463c1d215" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_019e4114-573d-77d1-9a06-000463c1d215_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ValuationTechniqueAxis_019e4114-573d-77d1-9a06-000463c1d215" xlink:to="loc_us-gaap_ValuationTechniqueDomain_019e4114-573d-77d1-9a06-000463c1d215_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ValuationTechniqueDomain_019e4114-573d-7293-a84e-c426543fb3d3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ValuationTechniqueDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ValuationTechniqueAxis_019e4114-573d-77d1-9a06-000463c1d215" xlink:to="loc_us-gaap_ValuationTechniqueDomain_019e4114-573d-7293-a84e-c426543fb3d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_UnobservableInputAppraisalAdjustmentsMember_019e4114-573d-725d-880e-47777f61405b" xlink:href="mpb-20260630.xsd#mpb_UnobservableInputAppraisalAdjustmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ValuationTechniqueDomain_019e4114-573d-7293-a84e-c426543fb3d3" xlink:to="loc_mpb_UnobservableInputAppraisalAdjustmentsMember_019e4114-573d-725d-880e-47777f61405b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_019e4114-573d-7388-b787-99c0dee06a48" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-744d-84d9-46bebefc9dd7" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_019e4114-573d-7388-b787-99c0dee06a48" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansHeldForSaleMeasurementInput_019e4114-573d-777a-9a3e-5a7dbe8ff69b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoansHeldForSaleMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019e4114-573d-744d-84d9-46bebefc9dd7" xlink:to="loc_us-gaap_LoansHeldForSaleMeasurementInput_019e4114-573d-777a-9a3e-5a7dbe8ff69b" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/FairValueMeasurementScheduleofFairValuebyBalanceSheetGroupingDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#FairValueMeasurementScheduleofFairValuebyBalanceSheetGroupingDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/FairValueMeasurementScheduleofFairValuebyBalanceSheetGroupingDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019e4114-573d-738e-bd27-a9605452bad1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019e4114-573d-7822-88f5-294813af53a5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019e4114-573d-738e-bd27-a9605452bad1" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019e4114-573d-7822-88f5-294813af53a5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_019e4114-573d-70af-8c7d-9ff900265665" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019e4114-573d-7822-88f5-294813af53a5" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_019e4114-573d-70af-8c7d-9ff900265665" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019e4114-573d-70af-8c7d-9ff900265665_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_019e4114-573d-70af-8c7d-9ff900265665" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019e4114-573d-70af-8c7d-9ff900265665_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019e4114-573d-7369-97ab-0f5c06101ce3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_019e4114-573d-70af-8c7d-9ff900265665" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019e4114-573d-7369-97ab-0f5c06101ce3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019e4114-573d-7ea8-abe2-32dc05f8cae2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019e4114-573d-7369-97ab-0f5c06101ce3" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019e4114-573d-7ea8-abe2-32dc05f8cae2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_019e4114-573d-75e5-985a-1f4087a8be31" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019e4114-573d-7ea8-abe2-32dc05f8cae2" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_019e4114-573d-75e5-985a-1f4087a8be31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_019e4114-573d-7007-8827-2924ee1ab0ff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019e4114-573d-7ea8-abe2-32dc05f8cae2" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_019e4114-573d-7007-8827-2924ee1ab0ff" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7c2b-a8b8-702cdf63a556" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019e4114-573d-7822-88f5-294813af53a5" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7c2b-a8b8-702cdf63a556" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c2b-a8b8-702cdf63a556_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7c2b-a8b8-702cdf63a556" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7c2b-a8b8-702cdf63a556_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7f8f-a710-c16b126761da" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019e4114-573d-7c2b-a8b8-702cdf63a556" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7f8f-a710-c16b126761da" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019e4114-573d-771d-b0da-699a9f5086b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7f8f-a710-c16b126761da" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019e4114-573d-771d-b0da-699a9f5086b8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019e4114-573d-70a2-8375-46a6ae331ca6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7f8f-a710-c16b126761da" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019e4114-573d-70a2-8375-46a6ae331ca6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019e4114-573d-7ca8-8450-8ac2ba7b228e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019e4114-573d-7f8f-a710-c16b126761da" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019e4114-573d-7ca8-8450-8ac2ba7b228e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019e4114-573d-738e-bd27-a9605452bad1" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019e4114-573d-7fd1-ab75-00ff3e91d27b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019e4114-573d-7fd1-ab75-00ff3e91d27b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-573d-7074-a6bd-845020cb9a9b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleExcludingAccruedInterest_019e4114-573d-7074-a6bd-845020cb9a9b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HeldToMaturitySecuritiesFairValue_019e4114-573d-71e9-a9af-bb3d22eb52e5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_HeldToMaturitySecuritiesFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_HeldToMaturitySecuritiesFairValue_019e4114-573d-71e9-a9af-bb3d22eb52e5" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNi_019e4114-573d-7584-8388-0e6c6010fb0b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquitySecuritiesFvNi"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_EquitySecuritiesFvNi_019e4114-573d-7584-8388-0e6c6010fb0b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansHeldForSaleFairValueDisclosure_019e4114-573d-7c4f-9cf6-7253d19fbb81" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoansHeldForSaleFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_LoansHeldForSaleFairValueDisclosure_019e4114-573d-7c4f-9cf6-7253d19fbb81" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansReceivableFairValueDisclosure_019e4114-573d-7fdf-9b39-88c15f87a4f2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoansReceivableFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_LoansReceivableFairValueDisclosure_019e4114-573d-7fdf-9b39-88c15f87a4f2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentInFederalHomeLoanBankStockFairValueDisclosure_019e4114-573d-72a5-b719-e704445acb87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentInFederalHomeLoanBankStockFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_InvestmentInFederalHomeLoanBankStockFairValueDisclosure_019e4114-573d-72a5-b719-e704445acb87" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_AccruedInterestReceivableFairValueDisclosure_019e4114-573d-7ce2-91ec-e882b601f56c" xlink:href="mpb-20260630.xsd#mpb_AccruedInterestReceivableFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_mpb_AccruedInterestReceivableFairValueDisclosure_019e4114-573d-7ce2-91ec-e882b601f56c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeAssets_019e4114-573d-7e74-83ab-0a47acab6375" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialAssetsBalanceSheetGroupingsAbstract_019e4114-573d-79ac-87ca-66c755c56b9a" xlink:to="loc_us-gaap_DerivativeAssets_019e4114-573d-7e74-83ab-0a47acab6375" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019e4114-573d-738e-bd27-a9605452bad1" xlink:to="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsFairValueDisclosure_019e4114-573d-71a2-b7ed-dd7bea24f386" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepositsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:to="loc_us-gaap_DepositsFairValueDisclosure_019e4114-573d-71a2-b7ed-dd7bea24f386" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShorttermDebtFairValue_019e4114-573d-7a67-a819-55fac703cc9d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShorttermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:to="loc_us-gaap_ShorttermDebtFairValue_019e4114-573d-7a67-a819-55fac703cc9d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtFairValue_019e4114-573d-7eb9-b242-6ec0ce9fad69" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:to="loc_us-gaap_LongTermDebtFairValue_019e4114-573d-7eb9-b242-6ec0ce9fad69" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisSubordinatedDebtObligations_019e4114-573d-7963-b6bb-20e815f03a90" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisSubordinatedDebtObligations"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:to="loc_us-gaap_FairValueLiabilitiesMeasuredOnRecurringBasisSubordinatedDebtObligations_019e4114-573d-7963-b6bb-20e815f03a90" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesFairValueDisclosure_019e4114-573d-727e-ab55-d08a8aecd6c5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccruedLiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:to="loc_us-gaap_AccruedLiabilitiesFairValueDisclosure_019e4114-573d-727e-ab55-d08a8aecd6c5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateDerivativeLiabilitiesAtFairValue_019e4114-573d-7763-8aed-35b58d002344" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestRateDerivativeLiabilitiesAtFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancialInstrumentsFinancialLiabilitiesBalanceSheetGroupingsAbstract_019e4114-573d-7eae-977c-ff714e90f1f3" xlink:to="loc_us-gaap_InterestRateDerivativeLiabilitiesAtFairValue_019e4114-573d-7763-8aed-35b58d002344" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/CommitmentsandContingenciesNarrativeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#CommitmentsandContingenciesNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/CommitmentsandContingenciesNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommitmentsContingenciesAndGuaranteesLineItems_019e4114-573d-79b4-9335-b55ed7fce5ea" xlink:href="mpb-20260630.xsd#mpb_CommitmentsContingenciesAndGuaranteesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommitmentsContingenciesAndGuaranteesTable_019e4114-573d-73cc-9bce-80fb5a6897b3" xlink:href="mpb-20260630.xsd#mpb_CommitmentsContingenciesAndGuaranteesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_mpb_CommitmentsContingenciesAndGuaranteesLineItems_019e4114-573d-79b4-9335-b55ed7fce5ea" xlink:to="loc_mpb_CommitmentsContingenciesAndGuaranteesTable_019e4114-573d-73cc-9bce-80fb5a6897b3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_019e4114-573d-7fd4-b543-c238cb8998bf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_mpb_CommitmentsContingenciesAndGuaranteesTable_019e4114-573d-73cc-9bce-80fb5a6897b3" xlink:to="loc_us-gaap_CreditFacilityAxis_019e4114-573d-7fd4-b543-c238cb8998bf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019e4114-573d-7fd4-b543-c238cb8998bf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_019e4114-573d-7fd4-b543-c238cb8998bf" xlink:to="loc_us-gaap_CreditFacilityDomain_019e4114-573d-7fd4-b543-c238cb8998bf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019e4114-573d-76fb-ae03-36c96341042d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_019e4114-573d-7fd4-b543-c238cb8998bf" xlink:to="loc_us-gaap_CreditFacilityDomain_019e4114-573d-76fb-ae03-36c96341042d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StandbyLettersOfCreditMember_019e4114-573d-71f3-8696-eb4381270510" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StandbyLettersOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019e4114-573d-76fb-ae03-36c96341042d" xlink:to="loc_us-gaap_StandbyLettersOfCreditMember_019e4114-573d-71f3-8696-eb4381270510" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskCreditRiskFinancialInstrumentMaximumExposure_019e4114-573d-72ab-86ca-b07b5e9ec582" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConcentrationRiskCreditRiskFinancialInstrumentMaximumExposure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_CommitmentsContingenciesAndGuaranteesLineItems_019e4114-573d-79b4-9335-b55ed7fce5ea" xlink:to="loc_us-gaap_ConcentrationRiskCreditRiskFinancialInstrumentMaximumExposure_019e4114-573d-72ab-86ca-b07b5e9ec582" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffBalanceSheetCreditLossLiability_019e4114-573d-756c-9b05-8e549378ab51" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OffBalanceSheetCreditLossLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_CommitmentsContingenciesAndGuaranteesLineItems_019e4114-573d-79b4-9335-b55ed7fce5ea" xlink:to="loc_us-gaap_OffBalanceSheetCreditLossLiability_019e4114-573d-756c-9b05-8e549378ab51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversal_019e4114-573d-7d79-81a2-a95397ce05c6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_CommitmentsContingenciesAndGuaranteesLineItems_019e4114-573d-79b4-9335-b55ed7fce5ea" xlink:to="loc_us-gaap_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversal_019e4114-573d-7d79-81a2-a95397ce05c6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversalNetOfAcquisitionAdjustment_019e4114-573d-7a64-a75a-29346b7bc45b" xlink:href="mpb-20260630.xsd#mpb_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversalNetOfAcquisitionAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_mpb_CommitmentsContingenciesAndGuaranteesLineItems_019e4114-573d-79b4-9335-b55ed7fce5ea" xlink:to="loc_mpb_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversalNetOfAcquisitionAdjustment_019e4114-573d-7a64-a75a-29346b7bc45b" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/CommitmentsandContingenciesScheduleofACLOBSbySegmentDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#CommitmentsandContingenciesScheduleofACLOBSbySegmentDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/CommitmentsandContingenciesScheduleofACLOBSbySegmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueOffBalanceSheetRisksDisclosureInformationLineItems_019e4114-573d-7352-8760-dec2029bea02" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueOffBalanceSheetRisksDisclosureInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFairValueOffBalanceSheetRisksTable_019e4114-573d-7f4d-9397-0cd135ae6a0d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFairValueOffBalanceSheetRisksTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueOffBalanceSheetRisksDisclosureInformationLineItems_019e4114-573d-7352-8760-dec2029bea02" xlink:to="loc_us-gaap_ScheduleOfFairValueOffBalanceSheetRisksTable_019e4114-573d-7f4d-9397-0cd135ae6a0d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-573d-7318-b6aa-bc4c9e79ab30" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFairValueOffBalanceSheetRisksTable_019e4114-573d-7f4d-9397-0cd135ae6a0d" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-573d-7318-b6aa-bc4c9e79ab30" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7318-b6aa-bc4c9e79ab30_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-573d-7318-b6aa-bc4c9e79ab30" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7318-b6aa-bc4c9e79ab30_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentByClassOfFinancingReceivableAxis_019e4114-573d-7318-b6aa-bc4c9e79ab30" xlink:to="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A14FamilyRentalMember_019e4114-573d-7824-98c9-f33b0e9bc74c" xlink:href="mpb-20260630.xsd#mpb_A14FamilyRentalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_A14FamilyRentalMember_019e4114-573d-7824-98c9-f33b0e9bc74c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-573d-786b-ab81-1081d012afd2" xlink:href="mpb-20260630.xsd#mpb_CommercialAndIndustrialLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_CommercialAndIndustrialLoansMember_019e4114-573d-786b-ab81-1081d012afd2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CRENonownerOccupiedMember_019e4114-573d-7c1d-b9cf-872d237761ef" xlink:href="mpb-20260630.xsd#mpb_CRENonownerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_CRENonownerOccupiedMember_019e4114-573d-7c1d-b9cf-872d237761ef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CREOwnerOccupiedMember_019e4114-573d-7b96-ad96-2204dcb55539" xlink:href="mpb-20260630.xsd#mpb_CREOwnerOccupiedMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_CREOwnerOccupiedMember_019e4114-573d-7b96-ad96-2204dcb55539" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ConsumerMember_019e4114-573d-70e6-8499-52b6f91c4d92" xlink:href="mpb-20260630.xsd#mpb_ConsumerMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_ConsumerMember_019e4114-573d-70e6-8499-52b6f91c4d92" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FarmlandMember_019e4114-573d-78d3-a50c-e584960d5d34" xlink:href="mpb-20260630.xsd#mpb_FarmlandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_FarmlandMember_019e4114-573d-78d3-a50c-e584960d5d34" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_HELOCAndJuniorLiensMember_019e4114-573d-7045-8fd2-c4208a0bbdfe" xlink:href="mpb-20260630.xsd#mpb_HELOCAndJuniorLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_HELOCAndJuniorLiensMember_019e4114-573d-7045-8fd2-c4208a0bbdfe" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MultifamilyMember_019e4114-573d-7fa2-885b-90da1725f077" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MultifamilyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_srt_MultifamilyMember_019e4114-573d-7fa2-885b-90da1725f077" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OtherConstructionAndLandMember_019e4114-573d-736d-9693-7217bd40e759" xlink:href="mpb-20260630.xsd#mpb_OtherConstructionAndLandMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_OtherConstructionAndLandMember_019e4114-573d-736d-9693-7217bd40e759" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ResidentialConstructionMember_019e4114-573d-7edb-984d-707b615db6a9" xlink:href="mpb-20260630.xsd#mpb_ResidentialConstructionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_ResidentialConstructionMember_019e4114-573d-7edb-984d-707b615db6a9" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ResidentialFirstLiensMember_019e4114-573d-773d-94e2-fed9c14e5c98" xlink:href="mpb-20260630.xsd#mpb_ResidentialFirstLiensMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FinancingReceivableRecordedInvestmentClassOfFinancingReceivableDomain_019e4114-573d-7e5c-a9e4-71262319af38" xlink:to="loc_mpb_ResidentialFirstLiensMember_019e4114-573d-773d-94e2-fed9c14e5c98" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffBalanceSheetCreditLossLiabilityRollforward_019e4114-573d-7212-81bd-6a3c64f2d5a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OffBalanceSheetCreditLossLiabilityRollforward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueOffBalanceSheetRisksDisclosureInformationLineItems_019e4114-573d-7352-8760-dec2029bea02" xlink:to="loc_us-gaap_OffBalanceSheetCreditLossLiabilityRollforward_019e4114-573d-7212-81bd-6a3c64f2d5a9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffBalanceSheetCreditLossLiability_019e4114-573d-7226-bd16-d420fb779b34" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OffBalanceSheetCreditLossLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OffBalanceSheetCreditLossLiabilityRollforward_019e4114-573d-7212-81bd-6a3c64f2d5a9" xlink:to="loc_us-gaap_OffBalanceSheetCreditLossLiability_019e4114-573d-7226-bd16-d420fb779b34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OffBalanceSheetCreditLossLiabilityInitialAllowanceForCreditLossOnAcquiredCommitments_019e6dc9-918d-79d9-a368-af3f76916baf" xlink:href="mpb-20260630.xsd#mpb_OffBalanceSheetCreditLossLiabilityInitialAllowanceForCreditLossOnAcquiredCommitments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OffBalanceSheetCreditLossLiabilityRollforward_019e4114-573d-7212-81bd-6a3c64f2d5a9" xlink:to="loc_mpb_OffBalanceSheetCreditLossLiabilityInitialAllowanceForCreditLossOnAcquiredCommitments_019e6dc9-918d-79d9-a368-af3f76916baf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversal_019e4114-573d-731a-924f-e8f8b8bb6ac8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversal"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OffBalanceSheetCreditLossLiabilityRollforward_019e4114-573d-7212-81bd-6a3c64f2d5a9" xlink:to="loc_us-gaap_OffBalanceSheetCreditLossLiabilityCreditLossExpenseReversal_019e4114-573d-731a-924f-e8f8b8bb6ac8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OffBalanceSheetCreditLossLiability_019e4114-573d-7471-a8fd-e9edea5ee3e8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OffBalanceSheetCreditLossLiability"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/DebtNarrativeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#DebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/DebtNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7931-a7c8-3e617734315c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7931-a7c8-3e617734315c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAxis_019e4114-573d-7333-9beb-47a0bc7fa7d7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7931-a7c8-3e617734315c" xlink:to="loc_us-gaap_LineOfCreditFacilityAxis_019e4114-573d-7333-9beb-47a0bc7fa7d7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_019e4114-573d-7333-9beb-47a0bc7fa7d7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_019e4114-573d-7333-9beb-47a0bc7fa7d7" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_019e4114-573d-7333-9beb-47a0bc7fa7d7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_019e4114-573d-73c4-a073-1e513ddad20b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_019e4114-573d-7333-9beb-47a0bc7fa7d7" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_019e4114-573d-73c4-a073-1e513ddad20b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_OtherCorrespondentBanksMember_019e4114-573d-7811-b7da-6b46956992f8" xlink:href="mpb-20260630.xsd#mpb_OtherCorrespondentBanksMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLenderDomain_019e4114-573d-73c4-a073-1e513ddad20b" xlink:to="loc_mpb_OtherCorrespondentBanksMember_019e4114-573d-7811-b7da-6b46956992f8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermBorrowings_019e4114-573d-7a47-acf3-9e79fec57fc5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_ShortTermBorrowings_019e4114-573d-7a47-acf3-9e79fec57fc5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_MaturityOfFederalFundsPurchasedFromCorrespondentBanks_019e4114-573d-7a73-9f64-5860fae267a8" xlink:href="mpb-20260630.xsd#mpb_MaturityOfFederalFundsPurchasedFromCorrespondentBanks"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_mpb_MaturityOfFederalFundsPurchasedFromCorrespondentBanks_019e4114-573d-7a73-9f64-5860fae267a8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresMaximumAmountAvailable_019e4114-573d-7b09-a492-0dd0e2e06cbd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresMaximumAmountAvailable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresMaximumAmountAvailable_019e4114-573d-7b09-a492-0dd0e2e06cbd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresAmountOfAvailableUnusedFunds_019e4114-573d-7477-ab04-2339b6dd0494" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresAmountOfAvailableUnusedFunds"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesGeneralDebtObligationsDisclosuresAmountOfAvailableUnusedFunds_019e4114-573d-7477-ab04-2339b6dd0494" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FederalHomeLoanBankAdvancesGeneralDebtObligationsMaximumAmountAvailableNetOfDepositsAndAdvances_019e4114-573d-73f4-bea0-8244f290d8d9" xlink:href="mpb-20260630.xsd#mpb_FederalHomeLoanBankAdvancesGeneralDebtObligationsMaximumAmountAvailableNetOfDepositsAndAdvances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_mpb_FederalHomeLoanBankAdvancesGeneralDebtObligationsMaximumAmountAvailableNetOfDepositsAndAdvances_019e4114-573d-73f4-bea0-8244f290d8d9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_019e4114-573d-7dbf-8a1d-3d77756ce20d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_LineOfCreditFacilityRemainingBorrowingCapacity_019e4114-573d-7dbf-8a1d-3d77756ce20d" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_AmountOfDrawingsOutstandingOnShortTermBorrowings_019e4114-573d-7aa1-8baa-5331db792620" xlink:href="mpb-20260630.xsd#mpb_AmountOfDrawingsOutstandingOnShortTermBorrowings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_mpb_AmountOfDrawingsOutstandingOnShortTermBorrowings_019e4114-573d-7aa1-8baa-5331db792620" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_019e6db1-dcd3-7b00-a68c-c39f10cdeef0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FederalHomeLoanBankAdvancesLongTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_019e6db1-dcd3-7b00-a68c-c39f10cdeef0" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LettersOfCreditOutstandingAmount_019e4114-573d-7e4c-882e-56b42a9e2f75" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LettersOfCreditOutstandingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-770c-8d64-df524178abde" xlink:to="loc_us-gaap_LettersOfCreditOutstandingAmount_019e4114-573d-7e4c-882e-56b42a9e2f75" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/DebtScheduleofLongtermDebtOutstandingbyDueDateDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#DebtScheduleofLongtermDebtOutstandingbyDueDateDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/DebtScheduleofLongtermDebtOutstandingbyDueDateDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7be7-8b2f-dcaad2729761" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:to="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7be7-8b2f-dcaad2729761" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019e4114-573d-7436-8b0d-d8af4de1fcc4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7be7-8b2f-dcaad2729761" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019e4114-573d-7436-8b0d-d8af4de1fcc4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-7436-8b0d-d8af4de1fcc4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019e4114-573d-7436-8b0d-d8af4de1fcc4" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-7436-8b0d-d8af4de1fcc4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-712f-8883-33a9eed49045" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodAxis_019e4114-573d-7436-8b0d-d8af4de1fcc4" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-712f-8883-33a9eed49045" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodThreeMember_019e4114-573d-77dc-982c-acf12dd60afd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-712f-8883-33a9eed49045" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodThreeMember_019e4114-573d-77dc-982c-acf12dd60afd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodFourMember_019e4114-573d-75f2-8c79-71bf6637e56b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodFourMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-712f-8883-33a9eed49045" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodFourMember_019e4114-573d-75f2-8c79-71bf6637e56b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRedemptionPeriodFiveMember_019e4114-573d-7d17-9554-27121334245a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRedemptionPeriodFiveMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentRedemptionPeriodDomain_019e4114-573d-712f-8883-33a9eed49045" xlink:to="loc_us-gaap_DebtInstrumentRedemptionPeriodFiveMember_019e4114-573d-7d17-9554-27121334245a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FederalHomeLoanBankAdvancesLongTermBeforeFairValueAdjustment_019e4114-573d-7a5f-ac04-982fa9936bab" xlink:href="mpb-20260630.xsd#mpb_FederalHomeLoanBankAdvancesLongTermBeforeFairValueAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:to="loc_mpb_FederalHomeLoanBankAdvancesLongTermBeforeFairValueAdjustment_019e4114-573d-7a5f-ac04-982fa9936bab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FederalHomeLoanBankAdvancesLongTermAfterFairValueAdjustment_019e4114-573d-7415-a672-126c278e2598" xlink:href="mpb-20260630.xsd#mpb_FederalHomeLoanBankAdvancesLongTermAfterFairValueAdjustment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:to="loc_mpb_FederalHomeLoanBankAdvancesLongTermAfterFairValueAdjustment_019e4114-573d-7415-a672-126c278e2598" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FederalHomeLoanBankAdvancesLeaseObligationsIncludedInLongTermDebt_019e4114-573d-72cf-b567-caa2685f2449" xlink:href="mpb-20260630.xsd#mpb_FederalHomeLoanBankAdvancesLeaseObligationsIncludedInLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:to="loc_mpb_FederalHomeLoanBankAdvancesLeaseObligationsIncludedInLongTermDebt_019e4114-573d-72cf-b567-caa2685f2449" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_019e4114-573d-7be4-a11f-f38ffa1c6fd7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FederalHomeLoanBankAdvancesLongTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesLongTerm_019e4114-573d-7be4-a11f-f38ffa1c6fd7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankInterestRate_019e4114-573d-750a-b0bf-5dafc0edb337" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019e4114-573d-7d8f-9b57-8fb0a7cbc452" xlink:to="loc_us-gaap_FederalHomeLoanBankAdvancesBranchOfFHLBBankInterestRate_019e4114-573d-750a-b0bf-5dafc0edb337" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/SubordinatedDebtDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#SubordinatedDebtDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/SubordinatedDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7444-9740-dabba6738c93" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:to="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7444-9740-dabba6738c93" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-737f-97cf-63ba34c4bb31" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7444-9740-dabba6738c93" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-737f-97cf-63ba34c4bb31" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-737f-97cf-63ba34c4bb31_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-737f-97cf-63ba34c4bb31" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-737f-97cf-63ba34c4bb31_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-7a3d-9638-3b2a67650489" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-737f-97cf-63ba34c4bb31" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-7a3d-9638-3b2a67650489" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_RiverviewAcquisitionMember_019e4114-573d-7d19-9e86-8d39a9dfc596" xlink:href="mpb-20260630.xsd#mpb_RiverviewAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-7a3d-9638-3b2a67650489" xlink:to="loc_mpb_RiverviewAcquisitionMember_019e4114-573d-7d19-9e86-8d39a9dfc596" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019e4114-573d-78b2-b5f6-e8bf60e0370b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7444-9740-dabba6738c93" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019e4114-573d-78b2-b5f6-e8bf60e0370b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019e4114-573d-78b2-b5f6-e8bf60e0370b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019e4114-573d-78b2-b5f6-e8bf60e0370b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019e4114-573d-78b2-b5f6-e8bf60e0370b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019e4114-573d-72a8-8995-72ae72d4c282" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019e4114-573d-78b2-b5f6-e8bf60e0370b" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019e4114-573d-72a8-8995-72ae72d4c282" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedDebtMember_019e4114-573d-77b9-9980-d1efd65c1002" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubordinatedDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019e4114-573d-72a8-8995-72ae72d4c282" xlink:to="loc_us-gaap_SubordinatedDebtMember_019e4114-573d-77b9-9980-d1efd65c1002" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019e4114-573d-75b2-bda4-ed4c249ad285" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019e4114-573d-7444-9740-dabba6738c93" xlink:to="loc_us-gaap_DebtInstrumentAxis_019e4114-573d-75b2-bda4-ed4c249ad285" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019e4114-573d-75b2-bda4-ed4c249ad285_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019e4114-573d-75b2-bda4-ed4c249ad285" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019e4114-573d-75b2-bda4-ed4c249ad285_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019e4114-573d-739c-9a98-df2bf5e0b741" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019e4114-573d-75b2-bda4-ed4c249ad285" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019e4114-573d-739c-9a98-df2bf5e0b741" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_SubordinatedNotesDueDecemberTwoThousandAndThirtyMember_019e4114-573d-7d9b-88d2-ba47ee3309fa" xlink:href="mpb-20260630.xsd#mpb_SubordinatedNotesDueDecemberTwoThousandAndThirtyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019e4114-573d-739c-9a98-df2bf5e0b741" xlink:to="loc_mpb_SubordinatedNotesDueDecemberTwoThousandAndThirtyMember_019e4114-573d-7d9b-88d2-ba47ee3309fa" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_SubordinatedNotesDueMarchTwoThousandAndThirtyMember_019e4114-573d-79a3-a227-1139730bf467" xlink:href="mpb-20260630.xsd#mpb_SubordinatedNotesDueMarchTwoThousandAndThirtyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019e4114-573d-739c-9a98-df2bf5e0b741" xlink:to="loc_mpb_SubordinatedNotesDueMarchTwoThousandAndThirtyMember_019e4114-573d-79a3-a227-1139730bf467" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesLongTermDebt_019e4114-573d-795a-860a-a89def321d1a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:to="loc_us-gaap_BusinessCombinationRecognizedIdentifiableAssetsAcquiredAndLiabilitiesAssumedNoncurrentLiabilitiesLongTermDebt_019e4114-573d-795a-860a-a89def321d1a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationLongTermDebtFairValuePremium_019e4114-573d-7b69-b44d-6e8fd7565626" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationLongTermDebtFairValuePremium"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:to="loc_mpb_BusinessCombinationLongTermDebtFairValuePremium_019e4114-573d-7b69-b44d-6e8fd7565626" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019e4114-573d-7373-a7ae-98262ea6ec0b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019e4114-573d-7373-a7ae-98262ea6ec0b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt_019e4114-573d-7c48-9ae3-4979a80140b2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSubordinatedLongTermDebt_019e4114-573d-7c48-9ae3-4979a80140b2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubordinatedDebt_019e6d71-8818-7e48-a5ac-4e86f879195f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubordinatedDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019e4114-573d-7305-a62f-23b79da202b7" xlink:to="loc_us-gaap_SubordinatedDebt_019e6d71-8818-7e48-a5ac-4e86f879195f" xlink:type="arc" order="4"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/CommonStockandEquityIncentivePlansDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#CommonStockandEquityIncentivePlansDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/CommonStockandEquityIncentivePlansDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ShareRepurchaseProgramAxis_019e4114-573d-7e84-ae9a-cffa2a36f8bd" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ShareRepurchaseProgramAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:to="loc_srt_ShareRepurchaseProgramAxis_019e4114-573d-7e84-ae9a-cffa2a36f8bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ShareRepurchaseProgramDomain_019e4114-573d-7e84-ae9a-cffa2a36f8bd_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ShareRepurchaseProgramAxis_019e4114-573d-7e84-ae9a-cffa2a36f8bd" xlink:to="loc_srt_ShareRepurchaseProgramDomain_019e4114-573d-7e84-ae9a-cffa2a36f8bd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ShareRepurchaseProgramDomain_019e4114-573d-757d-9ae3-3a41f8bec39a" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ShareRepurchaseProgramDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ShareRepurchaseProgramAxis_019e4114-573d-7e84-ae9a-cffa2a36f8bd" xlink:to="loc_srt_ShareRepurchaseProgramDomain_019e4114-573d-757d-9ae3-3a41f8bec39a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_TreasuryStockRepurchaseProgramMember_019e4114-573d-7baf-93a1-506d64f4de9d" xlink:href="mpb-20260630.xsd#mpb_TreasuryStockRepurchaseProgramMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ShareRepurchaseProgramDomain_019e4114-573d-757d-9ae3-3a41f8bec39a" xlink:to="loc_mpb_TreasuryStockRepurchaseProgramMember_019e4114-573d-7baf-93a1-506d64f4de9d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_019e4114-573d-7a9b-a15c-31f3b42900b9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:to="loc_us-gaap_PlanNameAxis_019e4114-573d-7a9b-a15c-31f3b42900b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_019e4114-573d-7a9b-a15c-31f3b42900b9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_019e4114-573d-7a9b-a15c-31f3b42900b9" xlink:to="loc_us-gaap_PlanNameDomain_019e4114-573d-7a9b-a15c-31f3b42900b9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_019e4114-573d-701b-a407-612926a92a78" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_019e4114-573d-7a9b-a15c-31f3b42900b9" xlink:to="loc_us-gaap_PlanNameDomain_019e4114-573d-701b-a407-612926a92a78" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A2023StockIncentivePlanMember_019e4114-573d-745f-98f6-b0cf68fe090d" xlink:href="mpb-20260630.xsd#mpb_A2023StockIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_019e4114-573d-701b-a407-612926a92a78" xlink:to="loc_mpb_A2023StockIncentivePlanMember_019e4114-573d-745f-98f6-b0cf68fe090d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualAxis_019e4114-573d-7677-83f5-cbb89bd145d7" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:to="loc_srt_TitleOfIndividualAxis_019e4114-573d-7677-83f5-cbb89bd145d7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_019e4114-573d-7677-83f5-cbb89bd145d7_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_TitleOfIndividualAxis_019e4114-573d-7677-83f5-cbb89bd145d7" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_019e4114-573d-7677-83f5-cbb89bd145d7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_019e4114-573d-71ce-bd86-55bb991a2a00" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_TitleOfIndividualAxis_019e4114-573d-7677-83f5-cbb89bd145d7" xlink:to="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_019e4114-573d-71ce-bd86-55bb991a2a00" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_EmployeeMember_019e4114-573d-729f-a99b-fe453585cc5c" xlink:href="mpb-20260630.xsd#mpb_EmployeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_019e4114-573d-71ce-bd86-55bb991a2a00" xlink:to="loc_mpb_EmployeeMember_019e4114-573d-729f-a99b-fe453585cc5c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_DirectorMember_019e4114-573d-7427-91c7-58344d5ad48d" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_DirectorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_TitleOfIndividualWithRelationshipToEntityDomain_019e4114-573d-71ce-bd86-55bb991a2a00" xlink:to="loc_srt_DirectorMember_019e4114-573d-7427-91c7-58344d5ad48d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019e4114-573d-7acb-9968-f1285426c452" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:to="loc_srt_RangeAxis_019e4114-573d-7acb-9968-f1285426c452" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019e4114-573d-7acb-9968-f1285426c452_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019e4114-573d-7acb-9968-f1285426c452" xlink:to="loc_srt_RangeMember_019e4114-573d-7acb-9968-f1285426c452_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019e4114-573d-7f02-82cb-010b937ccae6" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019e4114-573d-7acb-9968-f1285426c452" xlink:to="loc_srt_RangeMember_019e4114-573d-7f02-82cb-010b937ccae6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019e4114-573d-77d9-b3ea-d657a1fbf74f" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e4114-573d-7f02-82cb-010b937ccae6" xlink:to="loc_srt_MinimumMember_019e4114-573d-77d9-b3ea-d657a1fbf74f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019e4114-573d-74ed-bc58-8da6ed33721d" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019e4114-573d-7f02-82cb-010b937ccae6" xlink:to="loc_srt_MaximumMember_019e4114-573d-74ed-bc58-8da6ed33721d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-7c33-af95-ff9c4bc3956b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-7c33-af95-ff9c4bc3956b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-7c33-af95-ff9c4bc3956b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-7c33-af95-ff9c4bc3956b" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-7c33-af95-ff9c4bc3956b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-705e-a047-ce506924602f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e4114-573d-7c33-af95-ff9c4bc3956b" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-705e-a047-ce506924602f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_WilliamPennAcquisitionMember_019e4114-573d-7e8b-a444-01a9cff4b3f6" xlink:href="mpb-20260630.xsd#mpb_WilliamPennAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e4114-573d-705e-a047-ce506924602f" xlink:to="loc_mpb_WilliamPennAcquisitionMember_019e4114-573d-7e8b-a444-01a9cff4b3f6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_019e4114-573d-75e6-8ae2-1ba8cab356a0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019e4114-573d-7df1-9f9e-ca1c7a1ab863" xlink:to="loc_us-gaap_AwardTypeAxis_019e4114-573d-75e6-8ae2-1ba8cab356a0" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-75e6-8ae2-1ba8cab356a0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_019e4114-573d-75e6-8ae2-1ba8cab356a0" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-75e6-8ae2-1ba8cab356a0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-7cca-8243-3542a31c9362" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_019e4114-573d-75e6-8ae2-1ba8cab356a0" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-7cca-8243-3542a31c9362" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019e4114-573d-70ae-835e-1bc1326e66fb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-7cca-8243-3542a31c9362" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019e4114-573d-70ae-835e-1bc1326e66fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_019e4114-573d-78ae-b502-d20e5479f8a7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-7cca-8243-3542a31c9362" xlink:to="loc_us-gaap_EmployeeStockOptionMember_019e4114-573d-78ae-b502-d20e5479f8a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockAppreciationRightsSARSMember_019e6d5f-6719-7afa-84ef-d181479e7415" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockAppreciationRightsSARSMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019e4114-573d-7cca-8243-3542a31c9362" xlink:to="loc_us-gaap_StockAppreciationRightsSARSMember_019e6d5f-6719-7afa-84ef-d181479e7415" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StockRepurchaseProgramAuthorizedAmount1_019e4114-573d-7172-95f7-b33f7bfa7f8d" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_StockRepurchaseProgramAuthorizedAmount1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_srt_StockRepurchaseProgramAuthorizedAmount1_019e4114-573d-7172-95f7-b33f7bfa7f8d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockRepurchasedDuringPeriodShares_019e4114-573d-7304-ba7b-9ac9afe599ab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockRepurchasedDuringPeriodShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_StockRepurchasedDuringPeriodShares_019e4114-573d-7304-ba7b-9ac9afe599ab" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_019e4114-573d-7cfd-9ddb-6ee1abb732fd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockAcquiredAverageCostPerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_TreasuryStockAcquiredAverageCostPerShare_019e4114-573d-7cfd-9ddb-6ee1abb732fd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_StockRepurchasedDuringPeriodCumulativeShares_019e4114-573d-73db-bad6-cf5d9ab16eb7" xlink:href="mpb-20260630.xsd#mpb_StockRepurchasedDuringPeriodCumulativeShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_mpb_StockRepurchasedDuringPeriodCumulativeShares_019e4114-573d-73db-bad6-cf5d9ab16eb7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_StockRepurchasedDuringPeriodCumulativePricePerShare_019e4114-573d-7d42-a668-3e114fb5da66" xlink:href="mpb-20260630.xsd#mpb_StockRepurchasedDuringPeriodCumulativePricePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_mpb_StockRepurchasedDuringPeriodCumulativePricePerShare_019e4114-573d-7d42-a668-3e114fb5da66" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_019e6d5a-c9b8-7bda-a942-8ecb747a5cba" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_019e6d5a-c9b8-7bda-a942-8ecb747a5cba" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_019e4114-573d-7ce1-9efe-a9476798706d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_019e4114-573d-7ce1-9efe-a9476798706d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_019e4114-573d-7f2d-82c7-538cad5d11d8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_019e4114-573d-7f2d-82c7-538cad5d11d8" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_019e4114-573d-7ee7-afc1-e13eef948c51" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_019e4114-573d-7ee7-afc1-e13eef948c51" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_019e4114-573d-7c6c-8485-f487d4b08a61" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_019e4114-573d-7c6c-8485-f487d4b08a61" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_019e4114-573d-7788-8736-4651a2b53107" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_019e4114-573d-7788-8736-4651a2b53107" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_019e4114-573d-73f9-8abb-b2b85fec6fa7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_019e4114-573d-73f9-8abb-b2b85fec6fa7" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_019e4114-573d-7d2a-ae7d-02531ba6f248" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_019e4114-573d-7d2a-ae7d-02531ba6f248" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationConsiderationTransferredEquityInterestShareIssuedAdditionalNumberOfShares_019e4114-573d-75c8-9479-9935082c8b1b" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationConsiderationTransferredEquityInterestShareIssuedAdditionalNumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_mpb_BusinessCombinationConsiderationTransferredEquityInterestShareIssuedAdditionalNumberOfShares_019e4114-573d-75c8-9479-9935082c8b1b" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedNumberOfShares_019e4114-573d-74c9-b505-aae12d544d41" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedNumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsNonvestedNumberOfShares_019e4114-573d-74c9-b505-aae12d544d41" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_019e4114-573d-7818-841f-017cc6ed64ee" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_019e4114-573d-7818-841f-017cc6ed64ee" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercisableMaximumAggregateValue_019e6d59-9c1d-7eb4-b91a-cedbb039e67f" xlink:href="mpb-20260630.xsd#mpb_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercisableMaximumAggregateValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019e4114-573d-7e22-9f47-f274f9a82f3f" xlink:to="loc_mpb_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercisableMaximumAggregateValue_019e6d59-9c1d-7eb4-b91a-cedbb039e67f" xlink:type="arc" order="16"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/EarningsPerShareNarrativeDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#EarningsPerShareNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/EarningsPerShareNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_019e6d2d-bed8-7b99-b26b-b7d3fe66d2b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e6d2d-bed8-77f6-a20a-517b9b7acdbb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e6d2d-bed8-7b99-b26b-b7d3fe66d2b8" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e6d2d-bed8-77f6-a20a-517b9b7acdbb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019e6d2d-bed8-75c8-bba1-2071902a6eb8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019e6d2d-bed8-77f6-a20a-517b9b7acdbb" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019e6d2d-bed8-75c8-bba1-2071902a6eb8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-75c8-bba1-2071902a6eb8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e6d2d-bed8-75c8-bba1-2071902a6eb8" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-75c8-bba1-2071902a6eb8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-7a7a-9b7d-5fbd94af1625" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019e6d2d-bed8-75c8-bba1-2071902a6eb8" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-7a7a-9b7d-5fbd94af1625" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_WilliamPennAcquisitionMember_019e6d2d-bed8-703b-838b-032b121a38b2" xlink:href="mpb-20260630.xsd#mpb_WilliamPennAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-7a7a-9b7d-5fbd94af1625" xlink:to="loc_mpb_WilliamPennAcquisitionMember_019e6d2d-bed8-703b-838b-032b121a38b2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_CumberlandAdvisorsInc.Member_019e73c0-3814-7e40-870e-41c107bb2f40" xlink:href="mpb-20260630.xsd#mpb_CumberlandAdvisorsInc.Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-7a7a-9b7d-5fbd94af1625" xlink:to="loc_mpb_CumberlandAdvisorsInc.Member_019e73c0-3814-7e40-870e-41c107bb2f40" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_A1stColonialAcquisitionMember_019eb747-b85c-7c7f-8c81-adb2464d567f" xlink:href="mpb-20260630.xsd#mpb_A1stColonialAcquisitionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019e6d2d-bed8-7a7a-9b7d-5fbd94af1625" xlink:to="loc_mpb_A1stColonialAcquisitionMember_019eb747-b85c-7c7f-8c81-adb2464d567f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019e4114-573d-7034-b94c-2bd01f8b4b27" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e6d2d-bed8-7b99-b26b-b7d3fe66d2b8" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019e4114-573d-7034-b94c-2bd01f8b4b27" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_019e6d2d-bed8-769b-9577-e1485b7aa557" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e6d2d-bed8-7b99-b26b-b7d3fe66d2b8" xlink:to="loc_us-gaap_BusinessAcquisitionEquityInterestsIssuedOrIssuableNumberOfSharesIssued_019e6d2d-bed8-769b-9577-e1485b7aa557" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_BusinessCombinationConsiderationTransferredEquityInterestShareIssuedAdditionalNumberOfShares_019e6d2d-bed8-75d3-9767-3067f6bf413a" xlink:href="mpb-20260630.xsd#mpb_BusinessCombinationConsiderationTransferredEquityInterestShareIssuedAdditionalNumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019e6d2d-bed8-7b99-b26b-b7d3fe66d2b8" xlink:to="loc_mpb_BusinessCombinationConsiderationTransferredEquityInterestShareIssuedAdditionalNumberOfShares_019e6d2d-bed8-75d3-9767-3067f6bf413a" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.midpennbank.com/role/SegmentReportingScheduleofSegmentReportingInformationDetails" xlink:type="simple" xlink:href="mpb-20260630.xsd#SegmentReportingScheduleofSegmentReportingInformationDetails"/>
  <link:definitionLink xlink:role="http://www.midpennbank.com/role/SegmentReportingScheduleofSegmentReportingInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019e4114-573d-71e7-8606-9edca9dc5966" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019e4114-573d-71e7-8606-9edca9dc5966" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_019e4114-573d-75f5-aa65-4fdaeb4e0494" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019e4114-573d-71e7-8606-9edca9dc5966" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_019e4114-573d-75f5-aa65-4fdaeb4e0494" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_019e4114-573d-75f5-aa65-4fdaeb4e0494_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_019e4114-573d-75f5-aa65-4fdaeb4e0494" xlink:to="loc_us-gaap_SegmentDomain_019e4114-573d-75f5-aa65-4fdaeb4e0494_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_019e4114-573d-7126-b691-8464358f77fd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_019e4114-573d-75f5-aa65-4fdaeb4e0494" xlink:to="loc_us-gaap_SegmentDomain_019e4114-573d-7126-b691-8464358f77fd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_ReportableSegmentMember_019e4114-573d-7844-9a27-187644efcc74" xlink:href="mpb-20260630.xsd#mpb_ReportableSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_019e4114-573d-7126-b691-8464358f77fd" xlink:to="loc_mpb_ReportableSegmentMember_019e4114-573d-7844-9a27-187644efcc74" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeExpenseNet_019e4114-573d-72e6-aac4-8f6dce513194" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestIncomeExpenseNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_InterestIncomeExpenseNet_019e4114-573d-72e6-aac4-8f6dce513194" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_mpb_FinancingReceivableCreditLossExpenseReversalAdjusted_019e4114-573d-79e8-81c8-e21a47e2feb7" xlink:href="mpb-20260630.xsd#mpb_FinancingReceivableCreditLossExpenseReversalAdjusted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_mpb_FinancingReceivableCreditLossExpenseReversalAdjusted_019e4114-573d-79e8-81c8-e21a47e2feb7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestIncome_019e4114-573d-7c1a-b960-4a86632e099a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_NoninterestIncome_019e4114-573d-7c1a-b960-4a86632e099a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoninterestExpense_019e4114-573d-7c19-b754-29c36205d60c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NoninterestExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_NoninterestExpense_019e4114-573d-7c19-b754-29c36205d60c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019e4114-573d-72d7-8c59-0aae6cc764e7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019e4114-573d-72d7-8c59-0aae6cc764e7" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e4114-573d-732f-aadd-97b9dd24f430" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_NetIncomeLoss_019e4114-573d-732f-aadd-97b9dd24f430" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_019e4114-573d-72e6-9ec5-d73986de39f5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Assets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019e4114-573d-7e2b-b6d3-10a02dd29cda" xlink:to="loc_us-gaap_Assets_019e4114-573d-72e6-9ec5-d73986de39f5" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://fasb.org/srt/role/srt-eedm/ExtensibleEnumerationLists" xlink:type="simple" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-roles-2026.xsd#eedm1"/>
  <link:definitionLink xlink:role="http://fasb.org/srt/role/srt-eedm/ExtensibleEnumerationLists" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestReceivable" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsAbstract" xlink:to="loc_us-gaap_InterestReceivable" xlink:type="arc" order="101"/>
  </link:definitionLink>
</link:linkbase>
