<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019ef11c-bec5-7eb5-9cf8-dd952984b6d7,g:35326ea2-e820-41b4-8595-baca25ca0fc1-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.therealreal.com/role/CondensedStatementsofOperations" xlink:type="simple" xlink:href="real-20260630.xsd#CondensedStatementsofOperations"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/CondensedStatementsofOperations" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019ef11c-bfa2-75e3-97e0-41203b8f9f29" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_StatementTable_019ef11c-bfa2-75e3-97e0-41203b8f9f29" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_019ef11c-bfa2-7a9d-a1c7-be1469473f35" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019ef11c-bfa2-75e3-97e0-41203b8f9f29" xlink:to="loc_srt_ProductOrServiceAxis_019ef11c-bfa2-7a9d-a1c7-be1469473f35" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7a9d-a1c7-be1469473f35_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_019ef11c-bfa2-7a9d-a1c7-be1469473f35" xlink:to="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7a9d-a1c7-be1469473f35_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7ad9-91e1-fd15cd566b02" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_019ef11c-bfa2-7a9d-a1c7-be1469473f35" xlink:to="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7ad9-91e1-fd15cd566b02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServiceMember_019ef11c-bfa2-7483-9ec0-af946402c966" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7ad9-91e1-fd15cd566b02" xlink:to="loc_us-gaap_ServiceMember_019ef11c-bfa2-7483-9ec0-af946402c966" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_019ef11c-bfa2-779b-bee4-d4c4d89c8972" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7ad9-91e1-fd15cd566b02" xlink:to="loc_us-gaap_ProductMember_019ef11c-bfa2-779b-bee4-d4c4d89c8972" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShippingAndHandlingMember_019ef11c-bfa2-784b-b890-0a3c923bb8e3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShippingAndHandlingMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019ef11c-bfa2-7ad9-91e1-fd15cd566b02" xlink:to="loc_us-gaap_ShippingAndHandlingMember_019ef11c-bfa2-784b-b890-0a3c923bb8e3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_019ef11c-bfa2-7802-aaf6-60895b54e461" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_RevenuesAbstract_019ef11c-bfa2-7802-aaf6-60895b54e461" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019ef11c-bfa2-75a8-b9d1-47f676afd2c5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_019ef11c-bfa2-7802-aaf6-60895b54e461" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019ef11c-bfa2-75a8-b9d1-47f676afd2c5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfRevenueAbstract_019ef11c-bfa2-7342-a931-33bb9865349d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostOfRevenueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_CostOfRevenueAbstract_019ef11c-bfa2-7342-a931-33bb9865349d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfRevenue_019ef11c-bfa2-79ba-bbfc-9651d34bc9f7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostOfRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostOfRevenueAbstract_019ef11c-bfa2-7342-a931-33bb9865349d" xlink:to="loc_us-gaap_CostOfRevenue_019ef11c-bfa2-79ba-bbfc-9651d34bc9f7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_019ef11c-bfa2-7d72-a5c6-54df76e920be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_GrossProfit_019ef11c-bfa2-7d72-a5c6-54df76e920be" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_019ef11c-bfa2-7e17-b879-e3aa78ac4f31" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_OperatingExpensesAbstract_019ef11c-bfa2-7e17-b879-e3aa78ac4f31" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketingExpense_019ef11c-bfa2-7a7a-a4b2-55c364a4f0a0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019ef11c-bfa2-7e17-b879-e3aa78ac4f31" xlink:to="loc_us-gaap_MarketingExpense_019ef11c-bfa2-7a7a-a4b2-55c364a4f0a0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_OperationsAndTechnologyExpense_019ef11c-bfa2-7e5a-804e-f850884000db" xlink:href="real-20260630.xsd#real_OperationsAndTechnologyExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019ef11c-bfa2-7e17-b879-e3aa78ac4f31" xlink:to="loc_real_OperationsAndTechnologyExpense_019ef11c-bfa2-7e5a-804e-f850884000db" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019ef11c-bfa2-7e2f-bff4-557ca20cd8a8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019ef11c-bfa2-7e17-b879-e3aa78ac4f31" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019ef11c-bfa2-7e2f-bff4-557ca20cd8a8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_019ef11c-bfa2-7020-91ec-5f2418a39fc8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019ef11c-bfa2-7e17-b879-e3aa78ac4f31" xlink:to="loc_us-gaap_OperatingExpenses_019ef11c-bfa2-7020-91ec-5f2418a39fc8" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019ef11c-bfa2-7d61-bfb6-32c9e125cfde" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_OperatingIncomeLoss_019ef11c-bfa2-7d61-bfb6-32c9e125cfde" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAdjustmentOfWarrants_019ef11c-bfa2-7184-b6fa-2ef7c2e35183" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAdjustmentOfWarrants"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_FairValueAdjustmentOfWarrants_019ef11c-bfa2-7184-b6fa-2ef7c2e35183" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_019ef11c-bfa2-709e-885f-426909f91c43" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_019ef11c-bfa2-709e-885f-426909f91c43" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeInterest_019ef11c-bfa2-71b5-a7ff-bdf886c89d71" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentIncomeInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_InvestmentIncomeInterest_019ef11c-bfa2-71b5-a7ff-bdf886c89d71" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_019ef11c-bfa2-7992-bc35-89f43f7bdb2e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_InterestExpenseNonoperating_019ef11c-bfa2-7992-bc35-89f43f7bdb2e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_019ef11c-bfa2-7857-a76e-9498f21db7dd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_019ef11c-bfa2-7857-a76e-9498f21db7dd" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019ef11c-bfa2-70c3-8040-2ebd0738a174" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019ef11c-bfa2-70c3-8040-2ebd0738a174" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019ef11c-bfa2-7cef-9ee5-23b974aa6d12" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019ef11c-bfa2-7cef-9ee5-23b974aa6d12" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_019ef11c-bfa2-76c8-9ee4-df4685106058" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_019ef11c-bfa2-76c8-9ee4-df4685106058" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_019ef11c-bfa2-7b16-bf22-066a83f6994e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_EarningsPerShareBasic_019ef11c-bfa2-7b16-bf22-066a83f6994e" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_019ef11c-bfa2-79ea-b0ea-319a54c3e132" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_EarningsPerShareDiluted_019ef11c-bfa2-79ea-b0ea-319a54c3e132" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019ef11c-bfa2-7ee3-9b17-e93a93aad5a3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019ef11c-bfa2-7ee3-9b17-e93a93aad5a3" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019ef11c-bfa2-7d53-904d-b7084e92b40c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-bfa2-7b46-b5af-fa278092083f" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019ef11c-bfa2-7d53-904d-b7084e92b40c" xlink:type="arc" order="16"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/CondensedStatementsofStockholdersDeficit" xlink:type="simple" xlink:href="real-20260630.xsd#CondensedStatementsofStockholdersDeficit"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/CondensedStatementsofStockholdersDeficit" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019ef11c-c08f-741c-9ae6-acf944a64ece" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019ef11c-c08f-7d80-938c-6668a54d2668" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-c08f-741c-9ae6-acf944a64ece" xlink:to="loc_us-gaap_StatementTable_019ef11c-c08f-7d80-938c-6668a54d2668" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019ef11c-c08f-71bb-a93e-4cfee24c2c4e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019ef11c-c08f-7d80-938c-6668a54d2668" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019ef11c-c08f-71bb-a93e-4cfee24c2c4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-71bb-a93e-4cfee24c2c4e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019ef11c-c08f-71bb-a93e-4cfee24c2c4e" xlink:to="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-71bb-a93e-4cfee24c2c4e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-7a91-8ce6-30ee3d6f59a4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019ef11c-c08f-71bb-a93e-4cfee24c2c4e" xlink:to="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-7a91-8ce6-30ee3d6f59a4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_019ef11c-c08f-750b-8b9f-b0fecc0cb796" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-7a91-8ce6-30ee3d6f59a4" xlink:to="loc_us-gaap_CommonStockMember_019ef11c-c08f-750b-8b9f-b0fecc0cb796" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_019ef11c-c08f-7891-92c2-e590bf31fd1c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-7a91-8ce6-30ee3d6f59a4" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_019ef11c-c08f-7891-92c2-e590bf31fd1c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_019ef11c-c08f-7e01-9d0b-a44f67883a3f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019ef11c-c08f-7a91-8ce6-30ee3d6f59a4" xlink:to="loc_us-gaap_RetainedEarningsMember_019ef11c-c08f-7e01-9d0b-a44f67883a3f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019ef11c-c08f-741c-9ae6-acf944a64ece" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019ef11c-c08f-7cbb-a639-cc1f07fe5989" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_019ef11c-c08f-7cbb-a639-cc1f07fe5989" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019ef11c-c08f-7e88-8088-9a77f24b55a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockholdersEquity_019ef11c-c08f-7e88-8088-9a77f24b55a9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_StockIssuedDuringPeriodSharesSharesIssuedUponNetSettlementOfWarrantsExercised_019f17bb-4730-7625-a4cb-a34be9484b3c" xlink:href="real-20260630.xsd#real_StockIssuedDuringPeriodSharesSharesIssuedUponNetSettlementOfWarrantsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_real_StockIssuedDuringPeriodSharesSharesIssuedUponNetSettlementOfWarrantsExercised_019f17bb-4730-7625-a4cb-a34be9484b3c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_StockIssuedDuringPeriodValueSharesIssuedUponNetSettlementOfWarrantsExercised_019f17bb-fb1c-782a-bae0-0d788d1f7369" xlink:href="real-20260630.xsd#real_StockIssuedDuringPeriodValueSharesIssuedUponNetSettlementOfWarrantsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_real_StockIssuedDuringPeriodValueSharesIssuedUponNetSettlementOfWarrantsExercised_019f17bb-fb1c-782a-bae0-0d788d1f7369" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_AdjustmentsToAdditionalPaidInCapitalCallOptionsSettledInEquityAmount_019f17c5-95b6-7a6c-8bcb-65d07c6a8580" xlink:href="real-20260630.xsd#real_AdjustmentsToAdditionalPaidInCapitalCallOptionsSettledInEquityAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_real_AdjustmentsToAdditionalPaidInCapitalCallOptionsSettledInEquityAmount_019f17c5-95b6-7a6c-8bcb-65d07c6a8580" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_019ef11c-c08f-7589-b9b5-590c522ef66f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_019ef11c-c08f-7589-b9b5-590c522ef66f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_019ef11c-c08f-7ac8-bec2-3f174d317e99" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_019ef11c-c08f-7ac8-bec2-3f174d317e99" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_019ef11c-c08f-778d-8315-82b87eac1ecd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardNetOfForfeitures_019ef11c-c08f-778d-8315-82b87eac1ecd" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_019ef11c-c08f-7011-8385-2d886f57eb25" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueRestrictedStockAwardNetOfForfeitures_019ef11c-c08f-7011-8385-2d886f57eb25" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_019ef11c-c08f-7641-b34d-0f7ebad77216" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesEmployeeStockPurchasePlans_019ef11c-c08f-7641-b34d-0f7ebad77216" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_019ef11c-c08f-78ed-83da-2a8fd67309f2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueEmployeeStockPurchasePlan_019ef11c-c08f-78ed-83da-2a8fd67309f2" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_019ef11c-c08f-70b7-a88c-de3945067ef5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_019ef11c-c08f-70b7-a88c-de3945067ef5" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019ef11c-c08f-7345-8249-104f89ad7ee3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019ef11c-c08f-750a-90ac-679552a488c0" xlink:to="loc_us-gaap_NetIncomeLoss_019ef11c-c08f-7345-8249-104f89ad7ee3" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019ef11c-c08f-793c-acb0-fc05db8e0c15" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019ef11c-c08f-70bc-a287-639af0e18655" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/SummaryofSignificantAccountingPoliciesAdditionalInformationDetails" xlink:type="simple" xlink:href="real-20260630.xsd#SummaryofSignificantAccountingPoliciesAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/SummaryofSignificantAccountingPoliciesAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:href="real-20260630.xsd#real_SummaryOfSignificantAccountingPoliciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_SummaryOfSignificantAccountingPoliciesTable_019ef11c-c08f-7aec-bf4d-c4562aa253b6" xlink:href="real-20260630.xsd#real_SummaryOfSignificantAccountingPoliciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_real_SummaryOfSignificantAccountingPoliciesTable_019ef11c-c08f-7aec-bf4d-c4562aa253b6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementOfFinancialPositionLocationBalanceAxis_019ef11c-c08f-70f4-b24b-ee42ab01b26b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementOfFinancialPositionLocationBalanceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesTable_019ef11c-c08f-7aec-bf4d-c4562aa253b6" xlink:to="loc_us-gaap_StatementOfFinancialPositionLocationBalanceAxis_019ef11c-c08f-70f4-b24b-ee42ab01b26b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UndistributedEarningsLossAllocatedToParticipatingSecuritiesBasic_019f17cf-f990-74e3-a5d8-fd4562edd78e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_UndistributedEarningsLossAllocatedToParticipatingSecuritiesBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_UndistributedEarningsLossAllocatedToParticipatingSecuritiesBasic_019f17cf-f990-74e3-a5d8-fd4562edd78e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_019f17cf-f990-7c32-b081-ee93e447228e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_NumberOfOperatingSegments_019f17cf-f990-7c32-b081-ee93e447228e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_019f17cf-f990-7fbb-804b-72b89b389c5b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_NumberOfReportableSegments_019f17cf-f990-7fbb-804b-72b89b389c5b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_019ef11c-c08f-7bab-9683-a6972df0a837" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_RestrictedCash_019ef11c-c08f-7bab-9683-a6972df0a837" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWriteDown_019ef11c-c08f-7908-b8b9-c80469b78fcb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InventoryWriteDown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_InventoryWriteDown_019ef11c-c08f-7908-b8b9-c80469b78fcb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_InventoryReturnReservesAllowances_019ef11c-c08f-782e-ba39-742ade19033c" xlink:href="real-20260630.xsd#real_InventoryReturnReservesAllowances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_real_InventoryReturnReservesAllowances_019ef11c-c08f-782e-ba39-742ade19033c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_RightsToRecoverProductsFromCustomers_019ef11c-c08f-7d15-8478-4323c8f904df" xlink:href="real-20260630.xsd#real_RightsToRecoverProductsFromCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_real_RightsToRecoverProductsFromCustomers_019ef11c-c08f-7d15-8478-4323c8f904df" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/SummaryofSignificantAccountingPoliciesAdditionalInformationDetails_1" xlink:type="simple" xlink:href="real-20260630.xsd#SummaryofSignificantAccountingPoliciesAdditionalInformationDetails_1"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/SummaryofSignificantAccountingPoliciesAdditionalInformationDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:href="real-20260630.xsd#real_SummaryOfSignificantAccountingPoliciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_SummaryOfSignificantAccountingPoliciesTable_019ef11c-c08f-7aec-bf4d-c4562aa253b6" xlink:href="real-20260630.xsd#real_SummaryOfSignificantAccountingPoliciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_real_SummaryOfSignificantAccountingPoliciesTable_019ef11c-c08f-7aec-bf4d-c4562aa253b6" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UndistributedEarningsLossAllocatedToParticipatingSecuritiesBasic_019f17cf-f990-74e3-a5d8-fd4562edd78e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_UndistributedEarningsLossAllocatedToParticipatingSecuritiesBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_UndistributedEarningsLossAllocatedToParticipatingSecuritiesBasic_019f17cf-f990-74e3-a5d8-fd4562edd78e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_019f17cf-f990-7c32-b081-ee93e447228e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_NumberOfOperatingSegments_019f17cf-f990-7c32-b081-ee93e447228e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_019f17cf-f990-7fbb-804b-72b89b389c5b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_NumberOfReportableSegments_019f17cf-f990-7fbb-804b-72b89b389c5b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_019ef11c-c08f-7bab-9683-a6972df0a837" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_RestrictedCash_019ef11c-c08f-7bab-9683-a6972df0a837" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryWriteDown_019ef11c-c08f-7908-b8b9-c80469b78fcb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InventoryWriteDown"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_us-gaap_InventoryWriteDown_019ef11c-c08f-7908-b8b9-c80469b78fcb" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_InventoryReturnReservesAllowances_019ef11c-c08f-782e-ba39-742ade19033c" xlink:href="real-20260630.xsd#real_InventoryReturnReservesAllowances"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_real_InventoryReturnReservesAllowances_019ef11c-c08f-782e-ba39-742ade19033c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_RightsToRecoverProductsFromCustomers_019ef11c-c08f-7d15-8478-4323c8f904df" xlink:href="real-20260630.xsd#real_RightsToRecoverProductsFromCustomers"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_SummaryOfSignificantAccountingPoliciesLineItems_019ef11c-c08f-7fd0-ba0a-5d4cccbad48a" xlink:to="loc_real_RightsToRecoverProductsFromCustomers_019ef11c-c08f-7d15-8478-4323c8f904df" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/CashandCashEquivalentsDetails" xlink:type="simple" xlink:href="real-20260630.xsd#CashandCashEquivalentsDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/CashandCashEquivalentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsLineItems_019ef11c-c08f-7bb9-952a-40f4cacff2fe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_019ef11c-c08f-7fb8-a2a0-f78af607c1b1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfCashAndCashEquivalentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_019ef11c-c08f-7bb9-952a-40f4cacff2fe" xlink:to="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_019ef11c-c08f-7fb8-a2a0-f78af607c1b1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_019ef11c-c08f-7038-9485-b51302c70ae0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfCashAndCashEquivalentsTable_019ef11c-c08f-7fb8-a2a0-f78af607c1b1" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_019ef11c-c08f-7038-9485-b51302c70ae0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7038-9485-b51302c70ae0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_019ef11c-c08f-7038-9485-b51302c70ae0" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7038-9485-b51302c70ae0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7f63-8c4a-ce6d14ebf16f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsAxis_019ef11c-c08f-7038-9485-b51302c70ae0" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7f63-8c4a-ce6d14ebf16f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_RestrictedCashMember_019ef11c-c08f-7e32-9004-3fc8b3ae6005" xlink:href="real-20260630.xsd#real_RestrictedCashMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7f63-8c4a-ce6d14ebf16f" xlink:to="loc_real_RestrictedCashMember_019ef11c-c08f-7e32-9004-3fc8b3ae6005" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashMember_019ef11c-c08f-7f70-b92d-3be582baae8f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7f63-8c4a-ce6d14ebf16f" xlink:to="loc_us-gaap_CashMember_019ef11c-c08f-7f70-b92d-3be582baae8f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_019ef11c-c08f-7eec-932f-5deeaa3b7eb5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_019ef11c-c08f-7f63-8c4a-ce6d14ebf16f" xlink:to="loc_us-gaap_MoneyMarketFundsMember_019ef11c-c08f-7eec-932f-5deeaa3b7eb5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_CashAndCashEquivalentsUnrealizedGain_019ef11c-c08f-7a84-a96d-60a805ca6e29" xlink:href="real-20260630.xsd#real_CashAndCashEquivalentsUnrealizedGain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_019ef11c-c08f-7bb9-952a-40f4cacff2fe" xlink:to="loc_real_CashAndCashEquivalentsUnrealizedGain_019ef11c-c08f-7a84-a96d-60a805ca6e29" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_CashAndCashEquivalentsUnrealizedLoss_019ef11c-c08f-7171-9849-9996d07b5715" xlink:href="real-20260630.xsd#real_CashAndCashEquivalentsUnrealizedLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_019ef11c-c08f-7bb9-952a-40f4cacff2fe" xlink:to="loc_real_CashAndCashEquivalentsUnrealizedLoss_019ef11c-c08f-7171-9849-9996d07b5715" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019ef11c-c08f-7bd2-a95e-145f2ad3cd56" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_019ef11c-c08f-7bb9-952a-40f4cacff2fe" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019ef11c-c08f-7bd2-a95e-145f2ad3cd56" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019ef11c-c08f-707f-8c30-1d20ae23d13b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CashAndCashEquivalentsLineItems_019ef11c-c08f-7bb9-952a-40f4cacff2fe" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019ef11c-c08f-707f-8c30-1d20ae23d13b" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/FairValueMeasurementScheduleofFinancialInstrumentsMeasuredatFairValueDetails" xlink:type="simple" xlink:href="real-20260630.xsd#FairValueMeasurementScheduleofFinancialInstrumentsMeasuredatFairValueDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/FairValueMeasurementScheduleofFinancialInstrumentsMeasuredatFairValueDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-766a-b8f3-e350db805c0c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7b73-a59c-4530a69b9ed7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-766a-b8f3-e350db805c0c" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7b73-a59c-4530a69b9ed7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByAssetClassAxis_019ef11c-c08f-7fde-bf7e-bd620cb23383" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByAssetClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7b73-a59c-4530a69b9ed7" xlink:to="loc_us-gaap_FairValueByAssetClassAxis_019ef11c-c08f-7fde-bf7e-bd620cb23383" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019ef11c-c08f-7fde-bf7e-bd620cb23383_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_019ef11c-c08f-7fde-bf7e-bd620cb23383" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019ef11c-c08f-7fde-bf7e-bd620cb23383_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019ef11c-c08f-75a2-9bb6-3c36fd88d773" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByAssetClassAxis_019ef11c-c08f-7fde-bf7e-bd620cb23383" xlink:to="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019ef11c-c08f-75a2-9bb6-3c36fd88d773" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_019ef11c-c08f-7434-8e1f-01f65d047dad" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsMeasuredOnRecurringBasisUnobservableInputReconciliationByAssetClassDomain_019ef11c-c08f-75a2-9bb6-3c36fd88d773" xlink:to="loc_us-gaap_MoneyMarketFundsMember_019ef11c-c08f-7434-8e1f-01f65d047dad" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019ef11c-c08f-7ff3-b1a5-9d5a94ddaef6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7b73-a59c-4530a69b9ed7" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019ef11c-c08f-7ff3-b1a5-9d5a94ddaef6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7ff3-b1a5-9d5a94ddaef6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019ef11c-c08f-7ff3-b1a5-9d5a94ddaef6" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7ff3-b1a5-9d5a94ddaef6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7f21-9042-aadfc2e91628" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019ef11c-c08f-7ff3-b1a5-9d5a94ddaef6" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7f21-9042-aadfc2e91628" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019ef11c-c08f-70b7-81a2-7f1cbb3112ff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7f21-9042-aadfc2e91628" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019ef11c-c08f-70b7-81a2-7f1cbb3112ff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019ef11c-c08f-7776-812b-83f97fcbd07f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7f21-9042-aadfc2e91628" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019ef11c-c08f-7776-812b-83f97fcbd07f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019ef11c-c08f-708f-a157-23d3632c2679" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019ef11c-c08f-7f21-9042-aadfc2e91628" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019ef11c-c08f-708f-a157-23d3632c2679" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosureAbstract_019ef11c-c08f-7294-9703-90578efa6886" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-766a-b8f3-e350db805c0c" xlink:to="loc_us-gaap_AssetsFairValueDisclosureAbstract_019ef11c-c08f-7294-9703-90578efa6886" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019ef11c-c08f-745f-8a4f-14f13361c494" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_019ef11c-c08f-7294-9703-90578efa6886" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019ef11c-c08f-745f-8a4f-14f13361c494" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_019ef11c-c08f-79c2-b32f-518bdb1144c2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AssetsFairValueDisclosureAbstract_019ef11c-c08f-7294-9703-90578efa6886" xlink:to="loc_us-gaap_AssetsFairValueDisclosure_019ef11c-c08f-79c2-b32f-518bdb1144c2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_019ef11c-c08f-753b-af35-117a6ab20d0d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesFairValueDisclosureAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-766a-b8f3-e350db805c0c" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_019ef11c-c08f-753b-af35-117a6ab20d0d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_WarrantLiabilityNoncurrent_019ef11c-c08f-7862-845b-f001e195a21d" xlink:href="real-20260630.xsd#real_WarrantLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_019ef11c-c08f-753b-af35-117a6ab20d0d" xlink:to="loc_real_WarrantLiabilityNoncurrent_019ef11c-c08f-7862-845b-f001e195a21d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_019ef11c-c08f-7e14-8e2e-c5b30b1bd90e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosureAbstract_019ef11c-c08f-753b-af35-117a6ab20d0d" xlink:to="loc_us-gaap_LiabilitiesFairValueDisclosure_019ef11c-c08f-7e14-8e2e-c5b30b1bd90e" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/FairValueMeasurementScheduleofCarryingAmountsandEstimatedFairValuesDetails" xlink:type="simple" xlink:href="real-20260630.xsd#FairValueMeasurementScheduleofCarryingAmountsandEstimatedFairValuesDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/FairValueMeasurementScheduleofCarryingAmountsandEstimatedFairValuesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019ef11c-c08f-7ac2-842e-3e28b054703c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019ef11c-c08f-7d86-bcbc-cf1f7f3228d7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByBalanceSheetGroupingTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019ef11c-c08f-7ac2-842e-3e28b054703c" xlink:to="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019ef11c-c08f-7d86-bcbc-cf1f7f3228d7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_019ef11c-c08f-7727-af27-cf49609f1808" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019ef11c-c08f-7d86-bcbc-cf1f7f3228d7" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_019ef11c-c08f-7727-af27-cf49609f1808" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019ef11c-c08f-7727-af27-cf49609f1808_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_019ef11c-c08f-7727-af27-cf49609f1808" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019ef11c-c08f-7727-af27-cf49609f1808_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019ef11c-c08f-7bc4-a75a-ae90024dbbdf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_019ef11c-c08f-7727-af27-cf49609f1808" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019ef11c-c08f-7bc4-a75a-ae90024dbbdf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019ef11c-c08f-7e00-a9c4-46e2f79ccf7d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019ef11c-c08f-7bc4-a75a-ae90024dbbdf" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019ef11c-c08f-7e00-a9c4-46e2f79ccf7d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_019ef11c-c08f-7b8f-8266-a738eacbf957" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CarryingReportedAmountFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019ef11c-c08f-7e00-a9c4-46e2f79ccf7d" xlink:to="loc_us-gaap_CarryingReportedAmountFairValueDisclosureMember_019ef11c-c08f-7b8f-8266-a738eacbf957" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_019ef11c-c08f-793f-ae61-491351f97e66" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019ef11c-c08f-7e00-a9c4-46e2f79ccf7d" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_019ef11c-c08f-793f-ae61-491351f97e66" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7981-b756-2f22e3c830c4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019ef11c-c08f-7d86-bcbc-cf1f7f3228d7" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7981-b756-2f22e3c830c4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7981-b756-2f22e3c830c4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7981-b756-2f22e3c830c4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7981-b756-2f22e3c830c4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-78b1-85bf-b1a27e510aa9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7981-b756-2f22e3c830c4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-78b1-85bf-b1a27e510aa9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_019ef11c-c08f-747f-91e2-31cb73a717bb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-78b1-85bf-b1a27e510aa9" xlink:to="loc_us-gaap_SeniorNotesMember_019ef11c-c08f-747f-91e2-31cb73a717bb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7245-95b8-52d3b7351026" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueByBalanceSheetGroupingTable_019ef11c-c08f-7d86-bcbc-cf1f7f3228d7" xlink:to="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7245-95b8-52d3b7351026" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7245-95b8-52d3b7351026_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7245-95b8-52d3b7351026" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7245-95b8-52d3b7351026_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77d7-a85f-2cd6bf655864" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7245-95b8-52d3b7351026" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77d7-a85f-2cd6bf655864" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-7f0f-a050-97de9edf2856" xlink:href="real-20260630.xsd#real_A2028ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77d7-a85f-2cd6bf655864" xlink:to="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-7f0f-a050-97de9edf2856" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2029ConvertibleSeniorNotesMember_019ef11c-c08f-78b4-8baa-b92e2fe71ee3" xlink:href="real-20260630.xsd#real_A2029ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77d7-a85f-2cd6bf655864" xlink:to="loc_real_A2029ConvertibleSeniorNotesMember_019ef11c-c08f-78b4-8baa-b92e2fe71ee3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-7c5d-81f1-88c8d0ced8c5" xlink:href="real-20260630.xsd#real_A2031ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77d7-a85f-2cd6bf655864" xlink:to="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-7c5d-81f1-88c8d0ced8c5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleNotesPayable_019ef11c-c08f-728c-8e93-21a1d7121963" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConvertibleNotesPayable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019ef11c-c08f-7ac2-842e-3e28b054703c" xlink:to="loc_us-gaap_ConvertibleNotesPayable_019ef11c-c08f-728c-8e93-21a1d7121963" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableFairValueDisclosure_019ef11c-c08f-7eb0-847d-bee0297aaed5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NotesPayableFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueBalanceSheetGroupingFinancialStatementCaptionsLineItems_019ef11c-c08f-7ac2-842e-3e28b054703c" xlink:to="loc_us-gaap_NotesPayableFairValueDisclosure_019ef11c-c08f-7eb0-847d-bee0297aaed5" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/FairValueMeasurementAdditionalInformationDetails" xlink:type="simple" xlink:href="real-20260630.xsd#FairValueMeasurementAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/FairValueMeasurementAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7609-a5ef-af3c51c67f08" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7609-a5ef-af3c51c67f08" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-76af-82f7-a0ab3ff1bdaf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7609-a5ef-af3c51c67f08" xlink:to="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-76af-82f7-a0ab3ff1bdaf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-76af-82f7-a0ab3ff1bdaf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-76af-82f7-a0ab3ff1bdaf" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-76af-82f7-a0ab3ff1bdaf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7380-89b3-831802f98d91" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-76af-82f7-a0ab3ff1bdaf" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7380-89b3-831802f98d91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-7ce6-9b3c-e8859b98846f" xlink:href="real-20260630.xsd#real_A2028ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7380-89b3-831802f98d91" xlink:to="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-7ce6-9b3c-e8859b98846f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2029ConvertibleSeniorNotesMember_019ef11c-c08f-758f-8d9d-12beb32db551" xlink:href="real-20260630.xsd#real_A2029ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7380-89b3-831802f98d91" xlink:to="loc_real_A2029ConvertibleSeniorNotesMember_019ef11c-c08f-758f-8d9d-12beb32db551" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-75dc-85d0-8774ea70d5d3" xlink:href="real-20260630.xsd#real_A2031ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7380-89b3-831802f98d91" xlink:to="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-75dc-85d0-8774ea70d5d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-70e0-b73f-b8ae6a8fc407" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019ef11c-c08f-7609-a5ef-af3c51c67f08" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-70e0-b73f-b8ae6a8fc407" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-70e0-b73f-b8ae6a8fc407_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-70e0-b73f-b8ae6a8fc407" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-70e0-b73f-b8ae6a8fc407_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7861-8c43-1e35bac0ed6d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-70e0-b73f-b8ae6a8fc407" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7861-8c43-1e35bac0ed6d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_019ef11c-c08f-7247-ac4e-8abbc486f53f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7861-8c43-1e35bac0ed6d" xlink:to="loc_us-gaap_SeniorNotesMember_019ef11c-c08f-7247-ac4e-8abbc486f53f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019ef11c-c08f-7168-9b91-df5ca027743e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019ef11c-c08f-7168-9b91-df5ca027743e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtInstrumentPayableInKindInterestPercentage_019ef11c-c08f-7c5f-9bfc-39418b7e45e9" xlink:href="real-20260630.xsd#real_DebtInstrumentPayableInKindInterestPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_real_DebtInstrumentPayableInKindInterestPercentage_019ef11c-c08f-7c5f-9bfc-39418b7e45e9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtInstrumentPayableInCashInterestPercentage_019ef11c-c08f-7a8e-b59c-d48dc00cdd04" xlink:href="real-20260630.xsd#real_DebtInstrumentPayableInCashInterestPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_real_DebtInstrumentPayableInCashInterestPercentage_019ef11c-c08f-7a8e-b59c-d48dc00cdd04" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_019ef11c-c08f-7729-b729-5224ab99162a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_019ef11c-c08f-7729-b729-5224ab99162a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_019ef11c-c08f-7910-b6ea-6a5105b4e5f2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_019ef11c-c08f-7910-b6ea-6a5105b4e5f2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_019ef11c-c08f-78ce-8724-915152df2e6b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_019ef11c-c08f-78ce-8724-915152df2e6b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ClassOfWarrantOrRightWarrantsExercisedOnCashlessBasisAndReclassifiedFromWarrantLiabilities_019f1859-e957-705f-8681-0fd870bdcf20" xlink:href="real-20260630.xsd#real_ClassOfWarrantOrRightWarrantsExercisedOnCashlessBasisAndReclassifiedFromWarrantLiabilities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_real_ClassOfWarrantOrRightWarrantsExercisedOnCashlessBasisAndReclassifiedFromWarrantLiabilities_019f1859-e957-705f-8681-0fd870bdcf20" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ClassOfWarrantOrRightNumberOfWarrantsSettledOnNetShareBasis_019f1859-e957-72cd-853b-0554675e2cc7" xlink:href="real-20260630.xsd#real_ClassOfWarrantOrRightNumberOfWarrantsSettledOnNetShareBasis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_real_ClassOfWarrantOrRightNumberOfWarrantsSettledOnNetShareBasis_019f1859-e957-72cd-853b-0554675e2cc7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_StockIssuedDuringPeriodSharesSharesIssuedUponNetSettlementOfWarrantsExercised_019f1859-e957-7482-9dfa-047883ffd389" xlink:href="real-20260630.xsd#real_StockIssuedDuringPeriodSharesSharesIssuedUponNetSettlementOfWarrantsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_real_StockIssuedDuringPeriodSharesSharesIssuedUponNetSettlementOfWarrantsExercised_019f1859-e957-7482-9dfa-047883ffd389" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfWarrantOrRightOutstanding_019f1859-e957-7abc-8dbd-e2e58f05945e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019ef11c-c08f-7041-b30d-de6f3feb58ee" xlink:to="loc_us-gaap_ClassOfWarrantOrRightOutstanding_019f1859-e957-7abc-8dbd-e2e58f05945e" xlink:type="arc" order="9"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/FairValueMeasurementScheduleofFairValueMeasurementInputsandValuationTechniquesDetails" xlink:type="simple" xlink:href="real-20260630.xsd#FairValueMeasurementScheduleofFairValueMeasurementInputsandValuationTechniquesDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/FairValueMeasurementScheduleofFairValueMeasurementInputsandValuationTechniquesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_019ef11c-c08f-7fc7-a8d3-dc21ce8bf74f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_019ef11c-c08f-7d5a-8a24-e7d7d09dec87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_019ef11c-c08f-7fc7-a8d3-dc21ce8bf74f" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_019ef11c-c08f-7d5a-8a24-e7d7d09dec87" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_019ef11c-c08f-7084-97bc-f1657a0a5aa9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesTable_019ef11c-c08f-7d5a-8a24-e7d7d09dec87" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_019ef11c-c08f-7084-97bc-f1657a0a5aa9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7084-97bc-f1657a0a5aa9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019ef11c-c08f-7084-97bc-f1657a0a5aa9" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7084-97bc-f1657a0a5aa9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019ef11c-c08f-7084-97bc-f1657a0a5aa9" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputSharePriceMember_019ef11c-c08f-7d86-9611-f380693379a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputSharePriceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:to="loc_us-gaap_MeasurementInputSharePriceMember_019ef11c-c08f-7d86-9611-f380693379a9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputExercisePriceMember_019ef11c-c08f-7cf0-9e00-cb9f7a83e5c3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputExercisePriceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:to="loc_us-gaap_MeasurementInputExercisePriceMember_019ef11c-c08f-7cf0-9e00-cb9f7a83e5c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputExpectedTermMember_019ef11c-c08f-7248-9c1e-36fc44713f3c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputExpectedTermMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:to="loc_us-gaap_MeasurementInputExpectedTermMember_019ef11c-c08f-7248-9c1e-36fc44713f3c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputPriceVolatilityMember_019ef11c-c08f-78c1-83a5-9883c69ba72b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputPriceVolatilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:to="loc_us-gaap_MeasurementInputPriceVolatilityMember_019ef11c-c08f-78c1-83a5-9883c69ba72b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputExpectedDividendRateMember_019ef11c-c08f-71e8-92f5-e014dba0ce9e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputExpectedDividendRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:to="loc_us-gaap_MeasurementInputExpectedDividendRateMember_019ef11c-c08f-71e8-92f5-e014dba0ce9e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputDiscountRateMember_019ef11c-c08f-71c8-b0f5-526a862da0fa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputDiscountRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019ef11c-c08f-7179-83fc-ff3164e6726d" xlink:to="loc_us-gaap_MeasurementInputDiscountRateMember_019ef11c-c08f-71c8-b0f5-526a862da0fa" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantsAndRightsOutstandingMeasurementInput_019ef11c-c08f-7c6a-ac55-57c4e80ee9d1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantsAndRightsOutstandingMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisValuationTechniquesLineItems_019ef11c-c08f-7fc7-a8d3-dc21ce8bf74f" xlink:to="loc_us-gaap_WarrantsAndRightsOutstandingMeasurementInput_019ef11c-c08f-7c6a-ac55-57c4e80ee9d1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/CondensedBalanceSheetComponentsScheduleofPropertyPlantandEquipmentDetails" xlink:type="simple" xlink:href="real-20260630.xsd#CondensedBalanceSheetComponentsScheduleofPropertyPlantandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/CondensedBalanceSheetComponentsScheduleofPropertyPlantandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019ef11c-c08f-7c5e-9184-ae4a224d4b7f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019ef11c-c08f-7d53-a899-e75e950dd699" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019ef11c-c08f-7c5e-9184-ae4a224d4b7f" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019ef11c-c08f-7d53-a899-e75e950dd699" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019ef11c-c08f-7377-8725-6db45548516d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019ef11c-c08f-7d53-a899-e75e950dd699" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019ef11c-c08f-7377-8725-6db45548516d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7377-8725-6db45548516d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019ef11c-c08f-7377-8725-6db45548516d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7377-8725-6db45548516d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7fc1-99b2-40dc8539b9d3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019ef11c-c08f-7377-8725-6db45548516d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7fc1-99b2-40dc8539b9d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareDevelopmentMember_019f1867-79f3-7f41-9867-49005c3e3964" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SoftwareDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7fc1-99b2-40dc8539b9d3" xlink:to="loc_us-gaap_SoftwareDevelopmentMember_019f1867-79f3-7f41-9867-49005c3e3964" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_FurnitureAndEquipmentMember_019ef11c-c08f-79e9-b531-19beec1d0f01" xlink:href="real-20260630.xsd#real_FurnitureAndEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7fc1-99b2-40dc8539b9d3" xlink:to="loc_real_FurnitureAndEquipmentMember_019ef11c-c08f-79e9-b531-19beec1d0f01" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AutomobilesMember_019ef11c-c08f-736a-992c-903236a28d64" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AutomobilesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7fc1-99b2-40dc8539b9d3" xlink:to="loc_us-gaap_AutomobilesMember_019ef11c-c08f-736a-992c-903236a28d64" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_019ef11c-c08f-7776-a524-b8272706b7a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019ef11c-c08f-7fc1-99b2-40dc8539b9d3" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_019ef11c-c08f-7776-a524-b8272706b7a9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_019ef11c-c08f-79c6-bbcb-3702434f7f97" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019ef11c-c08f-7c5e-9184-ae4a224d4b7f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_019ef11c-c08f-79c6-bbcb-3702434f7f97" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_019ef11c-c08f-7e41-8d03-585d874ed6e2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019ef11c-c08f-7c5e-9184-ae4a224d4b7f" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_019ef11c-c08f-7e41-8d03-585d874ed6e2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_019ef11c-c08f-7b9e-aadd-f8ba1cafae40" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019ef11c-c08f-7c5e-9184-ae4a224d4b7f" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_019ef11c-c08f-7b9e-aadd-f8ba1cafae40" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/NonconvertibleNotesNetAdditionalInformationDetails" xlink:type="simple" xlink:href="real-20260630.xsd#NonconvertibleNotesNetAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/NonconvertibleNotesNetAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:href="real-20260630.xsd#real_DebtDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureTable_019ef11c-c08f-7c0b-ad89-1d80e52940ca" xlink:href="real-20260630.xsd#real_DebtDisclosureTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_real_DebtDisclosureTable_019ef11c-c08f-7c0b-ad89-1d80e52940ca" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7bc6-9017-41317d8ce1bd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7c0b-ad89-1d80e52940ca" xlink:to="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7bc6-9017-41317d8ce1bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7bc6-9017-41317d8ce1bd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7bc6-9017-41317d8ce1bd" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7bc6-9017-41317d8ce1bd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-765d-86f3-01c6851b0251" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7bc6-9017-41317d8ce1bd" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-765d-86f3-01c6851b0251" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2025ConvertibleSeniorNotesMember_019ef11c-c08f-7c2d-bcf3-230401b7a240" xlink:href="real-20260630.xsd#real_A2025ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-765d-86f3-01c6851b0251" xlink:to="loc_real_A2025ConvertibleSeniorNotesMember_019ef11c-c08f-7c2d-bcf3-230401b7a240" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-7ccc-b58c-ae4eaeb95ac5" xlink:href="real-20260630.xsd#real_A2028ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-765d-86f3-01c6851b0251" xlink:to="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-7ccc-b58c-ae4eaeb95ac5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2029SeniorNotesMember_019ef11c-c08f-704f-b7bd-a7d6a45db2b9" xlink:href="real-20260630.xsd#real_A2029SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-765d-86f3-01c6851b0251" xlink:to="loc_real_A2029SeniorNotesMember_019ef11c-c08f-704f-b7bd-a7d6a45db2b9" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7e11-ad3b-c66801cf9b78" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7c0b-ad89-1d80e52940ca" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7e11-ad3b-c66801cf9b78" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7e11-ad3b-c66801cf9b78_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7e11-ad3b-c66801cf9b78" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7e11-ad3b-c66801cf9b78_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7575-a29d-85de3f6afd64" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019ef11c-c08f-7e11-ad3b-c66801cf9b78" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7575-a29d-85de3f6afd64" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_019ef11c-c08f-7f05-af14-6c650a657458" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019ef11c-c08f-7575-a29d-85de3f6afd64" xlink:to="loc_us-gaap_SeniorNotesMember_019ef11c-c08f-7f05-af14-6c650a657458" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019ef11c-c08f-703e-8f15-a8a58754f34c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019ef11c-c08f-703e-8f15-a8a58754f34c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRepurchasedFaceAmount_019ef11c-c08f-70a8-8d1a-7b86f470452d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRepurchasedFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_us-gaap_DebtInstrumentRepurchasedFaceAmount_019ef11c-c08f-70a8-8d1a-7b86f470452d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_019ef11c-c08f-7579-82fd-57d593e7a737" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_019ef11c-c08f-7579-82fd-57d593e7a737" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtInstrumentPayableInCashInterestPercentage_019ef11c-c08f-754a-b8a0-71a9256fc446" xlink:href="real-20260630.xsd#real_DebtInstrumentPayableInCashInterestPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_real_DebtInstrumentPayableInCashInterestPercentage_019ef11c-c08f-754a-b8a0-71a9256fc446" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtInstrumentPayableInKindInterestPercentage_019ef11c-c08f-75f5-a44b-e76d1dcb8ffa" xlink:href="real-20260630.xsd#real_DebtInstrumentPayableInKindInterestPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_real_DebtInstrumentPayableInKindInterestPercentage_019ef11c-c08f-75f5-a44b-e76d1dcb8ffa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtInstrumentAccruedPaidInKindInterestCapitalized_019ef11c-c08f-7cbc-92b1-de5a2aad89ed" xlink:href="real-20260630.xsd#real_DebtInstrumentAccruedPaidInKindInterestCapitalized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7e7b-aa5b-9b355dba5316" xlink:to="loc_real_DebtInstrumentAccruedPaidInKindInterestCapitalized_019ef11c-c08f-7cbc-92b1-de5a2aad89ed" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/NonconvertibleNotesNetScheduleofFutureMinimumPaymentsUnderNotesDetails" xlink:type="simple" xlink:href="real-20260630.xsd#NonconvertibleNotesNetScheduleofFutureMinimumPaymentsUnderNotesDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/NonconvertibleNotesNetScheduleofFutureMinimumPaymentsUnderNotesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:href="real-20260630.xsd#real_DebtDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureTable_019ef11c-c08f-7cd0-97a6-1218a81c8a67" xlink:href="real-20260630.xsd#real_DebtDisclosureTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_real_DebtDisclosureTable_019ef11c-c08f-7cd0-97a6-1218a81c8a67" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-72a0-9e28-efb6a461a1ca" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7cd0-97a6-1218a81c8a67" xlink:to="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-72a0-9e28-efb6a461a1ca" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-72a0-9e28-efb6a461a1ca_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-72a0-9e28-efb6a461a1ca" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-72a0-9e28-efb6a461a1ca_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77da-a032-3b21076be584" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-72a0-9e28-efb6a461a1ca" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77da-a032-3b21076be584" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2029SeniorNotesMember_019ef11c-c08f-7f04-a263-aa4500dd95a6" xlink:href="real-20260630.xsd#real_A2029SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-77da-a032-3b21076be584" xlink:to="loc_real_A2029SeniorNotesMember_019ef11c-c08f-7f04-a263-aa4500dd95a6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_019ef11c-c08f-7abf-b38b-bf97f7c9aa21" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_019ef11c-c08f-7abf-b38b-bf97f7c9aa21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_019ef11c-c08f-7646-8f86-579995ef5b59" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_019ef11c-c08f-7646-8f86-579995ef5b59" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_019ef11c-c08f-7cba-83e4-e152cbd5cedc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_019ef11c-c08f-7cba-83e4-e152cbd5cedc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_019ef11c-c08f-76fe-950d-4c78410cdb49" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_019ef11c-c08f-76fe-950d-4c78410cdb49" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_LongTermDebtMaturityIncludingInterest_019ef11c-c08f-7b21-804c-4f8591e73aee" xlink:href="real-20260630.xsd#real_LongTermDebtMaturityIncludingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_real_LongTermDebtMaturityIncludingInterest_019ef11c-c08f-7b21-804c-4f8591e73aee" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet_019ef11c-c08f-75f8-bb40-796305b32e64" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet_019ef11c-c08f-75f8-bb40-796305b32e64" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_AccruedPaidInKindInterest_019ef11c-c08f-7030-8726-59e05b9604e6" xlink:href="real-20260630.xsd#real_AccruedPaidInKindInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_real_AccruedPaidInKindInterest_019ef11c-c08f-7030-8726-59e05b9604e6" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_019ef11c-c08f-751c-a0e5-b284bf01b82a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7fcb-8f3a-8553865592ff" xlink:to="loc_us-gaap_LongTermDebt_019ef11c-c08f-751c-a0e5-b284bf01b82a" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/ConvertibleSeniorNotesNetAdditionalInformationDetails" xlink:type="simple" xlink:href="real-20260630.xsd#ConvertibleSeniorNotesNetAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/ConvertibleSeniorNotesNetAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_019ef11c-c08f-77e1-a70d-575bfb63b0a3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_DebtInstrumentTable_019ef11c-c08f-77e1-a70d-575bfb63b0a3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019f18ae-c02c-710e-b08c-3ff5f90fd007" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019ef11c-c08f-77e1-a70d-575bfb63b0a3" xlink:to="loc_us-gaap_DebtInstrumentAxis_019f18ae-c02c-710e-b08c-3ff5f90fd007" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-710e-b08c-3ff5f90fd007_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019f18ae-c02c-710e-b08c-3ff5f90fd007" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-710e-b08c-3ff5f90fd007_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-722a-9b47-2c06649f0b1b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019f18ae-c02c-710e-b08c-3ff5f90fd007" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-722a-9b47-2c06649f0b1b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2031ConvertibleSeniorNotesMember_019f18ae-c02c-75f2-b4ca-5d9afe194c28" xlink:href="real-20260630.xsd#real_A2031ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-722a-9b47-2c06649f0b1b" xlink:to="loc_real_A2031ConvertibleSeniorNotesMember_019f18ae-c02c-75f2-b4ca-5d9afe194c28" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2028ConvertibleSeniorNotesMember_019f18ae-c02c-7e2e-b5d4-f7609b3cc83e" xlink:href="real-20260630.xsd#real_A2028ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-722a-9b47-2c06649f0b1b" xlink:to="loc_real_A2028ConvertibleSeniorNotesMember_019f18ae-c02c-7e2e-b5d4-f7609b3cc83e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2025ConvertibleSeniorNotesMember_019f18ae-c02c-7a59-985d-4f0dbd7757c0" xlink:href="real-20260630.xsd#real_A2025ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019f18ae-c02c-722a-9b47-2c06649f0b1b" xlink:to="loc_real_A2025ConvertibleSeniorNotesMember_019f18ae-c02c-7a59-985d-4f0dbd7757c0" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019f18ae-c02c-711f-8be0-04133c25a9b4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019ef11c-c08f-77e1-a70d-575bfb63b0a3" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019f18ae-c02c-711f-8be0-04133c25a9b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-711f-8be0-04133c25a9b4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f18ae-c02c-711f-8be0-04133c25a9b4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-711f-8be0-04133c25a9b4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-7a26-8cf7-639c8b4ad57b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f18ae-c02c-711f-8be0-04133c25a9b4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-7a26-8cf7-639c8b4ad57b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertibleDebtMember_019f8f88-46c6-73bd-b44b-3a56e69fec30" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConvertibleDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-7a26-8cf7-639c8b4ad57b" xlink:to="loc_us-gaap_ConvertibleDebtMember_019f8f88-46c6-73bd-b44b-3a56e69fec30" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableOtherPayablesMember_019f18ae-c02c-787d-9a3c-983339da1670" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NotesPayableOtherPayablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-7a26-8cf7-639c8b4ad57b" xlink:to="loc_us-gaap_NotesPayableOtherPayablesMember_019f18ae-c02c-787d-9a3c-983339da1670" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SeniorNotesMember_019f4274-f221-7850-8893-5e324d29827a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019f18ae-c02c-7a26-8cf7-639c8b4ad57b" xlink:to="loc_us-gaap_SeniorNotesMember_019f4274-f221-7850-8893-5e324d29827a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_019f8f86-ec68-74dc-a1d3-5d3bff66018e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtConversionByUniqueDescriptionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019ef11c-c08f-77e1-a70d-575bfb63b0a3" xlink:to="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_019f8f86-ec68-74dc-a1d3-5d3bff66018e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionNameDomain_019f8f86-ec68-74dc-a1d3-5d3bff66018e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtConversionNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_019f8f86-ec68-74dc-a1d3-5d3bff66018e" xlink:to="loc_us-gaap_DebtConversionNameDomain_019f8f86-ec68-74dc-a1d3-5d3bff66018e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtConversionNameDomain_019f8f86-ec68-7bf2-9a71-9c3398e9f27c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtConversionNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtConversionByUniqueDescriptionAxis_019f8f86-ec68-74dc-a1d3-5d3bff66018e" xlink:to="loc_us-gaap_DebtConversionNameDomain_019f8f86-ec68-7bf2-9a71-9c3398e9f27c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_February2025NoteExchangeMember_019f8f87-2a6b-7009-a1da-23edc8441d41" xlink:href="real-20260630.xsd#real_February2025NoteExchangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtConversionNameDomain_019f8f86-ec68-7bf2-9a71-9c3398e9f27c" xlink:to="loc_real_February2025NoteExchangeMember_019f8f87-2a6b-7009-a1da-23edc8441d41" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_August2025NoteExchangeMember_019f8f87-6b55-7ad5-ab14-482bd3ef18e5" xlink:href="real-20260630.xsd#real_August2025NoteExchangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtConversionNameDomain_019f8f86-ec68-7bf2-9a71-9c3398e9f27c" xlink:to="loc_real_August2025NoteExchangeMember_019f8f87-6b55-7ad5-ab14-482bd3ef18e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_019f18ae-c02c-7488-8bb2-686fc254ca40" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_019f18ae-c02c-7488-8bb2-686fc254ca40" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentRepurchasedFaceAmount_019f8f8b-bb3e-7bb6-abf3-aabfe2638942" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRepurchasedFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_DebtInstrumentRepurchasedFaceAmount_019f8f8b-bb3e-7bb6-abf3-aabfe2638942" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfConvertibleDebt_019f18ae-c02c-7d38-8a25-543c54bfab98" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RepaymentsOfConvertibleDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_RepaymentsOfConvertibleDebt_019f18ae-c02c-7d38-8a25-543c54bfab98" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_019f18ae-c02c-765a-9cde-cadad8872997" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_LongTermDebt_019f18ae-c02c-765a-9cde-cadad8872997" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019f18ae-c02c-7982-b7a8-e859c0c1fe60" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019f18ae-c02c-7982-b7a8-e859c0c1fe60" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet_019f18ae-c02c-7cd4-87b2-d75090d56e3e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet_019f18ae-c02c-7cd4-87b2-d75090d56e3e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnamortizedDebtIssuanceExpense_019f18ae-c02c-75e0-a634-82f1b64142b7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_UnamortizedDebtIssuanceExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019f18ae-c02c-7272-8ac3-940afd22ce18" xlink:to="loc_us-gaap_UnamortizedDebtIssuanceExpense_019f18ae-c02c-75e0-a634-82f1b64142b7" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/ConvertibleSeniorNotesNetScheduleofAmountsRecordedinInterestExpenseRelatedtoNotesDetails" xlink:type="simple" xlink:href="real-20260630.xsd#ConvertibleSeniorNotesNetScheduleofAmountsRecordedinInterestExpenseRelatedtoNotesDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/ConvertibleSeniorNotesNetScheduleofAmountsRecordedinInterestExpenseRelatedtoNotesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7df3-a25b-127c11c85e41" xlink:href="real-20260630.xsd#real_DebtDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureTable_019ef11c-c08f-7d13-8f15-700c7dd76772" xlink:href="real-20260630.xsd#real_DebtDisclosureTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7df3-a25b-127c11c85e41" xlink:to="loc_real_DebtDisclosureTable_019ef11c-c08f-7d13-8f15-700c7dd76772" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-767f-979b-24ea4c02e806" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7d13-8f15-700c7dd76772" xlink:to="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-767f-979b-24ea4c02e806" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-767f-979b-24ea4c02e806_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-767f-979b-24ea4c02e806" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-767f-979b-24ea4c02e806_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-73f6-af99-df0a6e740a7a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-767f-979b-24ea4c02e806" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-73f6-af99-df0a6e740a7a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2025ConvertibleSeniorNotesMember_019ef11c-c08f-720b-af3c-cef2504dc50f" xlink:href="real-20260630.xsd#real_A2025ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-73f6-af99-df0a6e740a7a" xlink:to="loc_real_A2025ConvertibleSeniorNotesMember_019ef11c-c08f-720b-af3c-cef2504dc50f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-76b6-b79f-bfc916b81d91" xlink:href="real-20260630.xsd#real_A2028ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-73f6-af99-df0a6e740a7a" xlink:to="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-76b6-b79f-bfc916b81d91" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-70c2-9369-58f86d3838ad" xlink:href="real-20260630.xsd#real_A2031ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-73f6-af99-df0a6e740a7a" xlink:to="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-70c2-9369-58f86d3838ad" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019f18b2-90cd-719a-a4bb-70c225ca6be4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7d13-8f15-700c7dd76772" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019f18b2-90cd-719a-a4bb-70c225ca6be4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f18b2-90cd-719a-a4bb-70c225ca6be4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f18b2-90cd-719a-a4bb-70c225ca6be4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f18b2-90cd-719a-a4bb-70c225ca6be4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f18b2-90cd-7290-94b8-4aef16e60239" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f18b2-90cd-719a-a4bb-70c225ca6be4" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f18b2-90cd-7290-94b8-4aef16e60239" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableOtherPayablesMember_019f18b2-90cd-784e-8f63-8c5368014527" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NotesPayableOtherPayablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019f18b2-90cd-7290-94b8-4aef16e60239" xlink:to="loc_us-gaap_NotesPayableOtherPayablesMember_019f18b2-90cd-784e-8f63-8c5368014527" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebtExcludingAmortization_019ef11c-c08f-78af-aaa9-28cb8b648646" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseDebtExcludingAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7df3-a25b-127c11c85e41" xlink:to="loc_us-gaap_InterestExpenseDebtExcludingAmortization_019ef11c-c08f-78af-aaa9-28cb8b648646" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfFinancingCosts_019ef11c-c08f-7d28-9d14-1eefc2792727" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfFinancingCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7df3-a25b-127c11c85e41" xlink:to="loc_us-gaap_AmortizationOfFinancingCosts_019ef11c-c08f-7d28-9d14-1eefc2792727" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseDebt_019ef11c-c08f-7f41-b6f9-2e439441c34e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-7df3-a25b-127c11c85e41" xlink:to="loc_us-gaap_InterestExpenseDebt_019ef11c-c08f-7f41-b6f9-2e439441c34e" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/ConvertibleSeniorNotesNetScheduleofFutureMinimumPaymentsUnderNotesDetails" xlink:type="simple" xlink:href="real-20260630.xsd#ConvertibleSeniorNotesNetScheduleofFutureMinimumPaymentsUnderNotesDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/ConvertibleSeniorNotesNetScheduleofFutureMinimumPaymentsUnderNotesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:href="real-20260630.xsd#real_DebtDisclosureLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_DebtDisclosureTable_019ef11c-c08f-7c97-aa07-977a1018b702" xlink:href="real-20260630.xsd#real_DebtDisclosureTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_real_DebtDisclosureTable_019ef11c-c08f-7c97-aa07-977a1018b702" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7355-985d-09b03ed501ee" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7c97-aa07-977a1018b702" xlink:to="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7355-985d-09b03ed501ee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7355-985d-09b03ed501ee_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7355-985d-09b03ed501ee" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-7355-985d-09b03ed501ee_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-75de-8040-f5bffb78fdf9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019ef11c-c08f-7355-985d-09b03ed501ee" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-75de-8040-f5bffb78fdf9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-75bc-86d8-72fd50f30b0a" xlink:href="real-20260630.xsd#real_A2028ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-75de-8040-f5bffb78fdf9" xlink:to="loc_real_A2028ConvertibleSeniorNotesMember_019ef11c-c08f-75bc-86d8-72fd50f30b0a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-78da-9148-1a6b0dbbb903" xlink:href="real-20260630.xsd#real_A2031ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019ef11c-c08f-75de-8040-f5bffb78fdf9" xlink:to="loc_real_A2031ConvertibleSeniorNotesMember_019ef11c-c08f-78da-9148-1a6b0dbbb903" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019f18b3-ed09-7ef0-b828-4a4cc505e75f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_DebtDisclosureTable_019ef11c-c08f-7c97-aa07-977a1018b702" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019f18b3-ed09-7ef0-b828-4a4cc505e75f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f18b3-ed09-7ef0-b828-4a4cc505e75f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f18b3-ed09-7ef0-b828-4a4cc505e75f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f18b3-ed09-7ef0-b828-4a4cc505e75f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019f18b3-ed09-7929-acb4-f2128c5ca466" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019f18b3-ed09-7ef0-b828-4a4cc505e75f" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019f18b3-ed09-7929-acb4-f2128c5ca466" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NotesPayableOtherPayablesMember_019f18b3-ed09-7dda-891d-b27ecfd6ab18" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NotesPayableOtherPayablesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019f18b3-ed09-7929-acb4-f2128c5ca466" xlink:to="loc_us-gaap_NotesPayableOtherPayablesMember_019f18b3-ed09-7dda-891d-b27ecfd6ab18" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_019ef11c-c08f-70d3-84c9-3fb2f20f2074" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_019ef11c-c08f-70d3-84c9-3fb2f20f2074" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_019ef11c-c08f-7116-888b-3eb03c3426ba" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_019ef11c-c08f-7116-888b-3eb03c3426ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_019ef11c-c08f-761e-b426-a2171d3a5657" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_019ef11c-c08f-761e-b426-a2171d3a5657" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_019ef11c-c08f-71ab-9734-68fbd1d9dd8f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_019ef11c-c08f-71ab-9734-68fbd1d9dd8f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour_019ef11c-c08f-709d-86a0-01d5bd7a3b68" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFour_019ef11c-c08f-709d-86a0-01d5bd7a3b68" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive_019ef11c-c08f-7f2b-9be1-6b33a2efbdd9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearFive_019ef11c-c08f-7f2b-9be1-6b33a2efbdd9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_LongTermDebtMaturityIncludingInterest_019ef11c-c08f-7fa9-b62f-39bf0cb9873b" xlink:href="real-20260630.xsd#real_LongTermDebtMaturityIncludingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_real_LongTermDebtMaturityIncludingInterest_019ef11c-c08f-7fa9-b62f-39bf0cb9873b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet_019ef11c-c08f-7759-b099-8c387b341df7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_DebtInstrumentUnamortizedDiscountPremiumAndDebtIssuanceCostsNet_019ef11c-c08f-7759-b099-8c387b341df7" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_019ef11c-c08f-78ce-8b0e-b965a72f669b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_DebtDisclosureLineItems_019ef11c-c08f-79c4-8c44-bfd63fc9f81e" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_019ef11c-c08f-78ce-8b0e-b965a72f669b" xlink:type="arc" order="8"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/SharebasedCompensationPlansAdditionalInformationDetails" xlink:type="simple" xlink:href="real-20260630.xsd#SharebasedCompensationPlansAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/SharebasedCompensationPlansAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_019ef11c-c08f-75e7-a51e-e3a5fdb1f375" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:to="loc_us-gaap_AwardTypeAxis_019ef11c-c08f-75e7-a51e-e3a5fdb1f375" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-75e7-a51e-e3a5fdb1f375_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_019ef11c-c08f-75e7-a51e-e3a5fdb1f375" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-75e7-a51e-e3a5fdb1f375_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-70c1-a3b2-25fe4f397d0f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_019ef11c-c08f-75e7-a51e-e3a5fdb1f375" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-70c1-a3b2-25fe4f397d0f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PerformanceSharesMember_019ef11c-c08f-7734-95df-1514f62dbd8e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PerformanceSharesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-70c1-a3b2-25fe4f397d0f" xlink:to="loc_us-gaap_PerformanceSharesMember_019ef11c-c08f-7734-95df-1514f62dbd8e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_RestrictedStockUnitsAndPerformanceShareUnitsMember_019ef11c-c08f-751f-abba-1893baa7c95f" xlink:href="real-20260630.xsd#real_RestrictedStockUnitsAndPerformanceShareUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-70c1-a3b2-25fe4f397d0f" xlink:to="loc_real_RestrictedStockUnitsAndPerformanceShareUnitsMember_019ef11c-c08f-751f-abba-1893baa7c95f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_019ef11c-c08f-79af-84b8-4830215c773f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-70c1-a3b2-25fe4f397d0f" xlink:to="loc_us-gaap_EmployeeStockOptionMember_019ef11c-c08f-79af-84b8-4830215c773f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019ef11c-c08f-794f-a585-64d526e931e1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019ef11c-c08f-70c1-a3b2-25fe4f397d0f" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019ef11c-c08f-794f-a585-64d526e931e1" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_019ef11c-c08f-75f1-b087-4d31c616274c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:to="loc_us-gaap_PlanNameAxis_019ef11c-c08f-75f1-b087-4d31c616274c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_019ef11c-c08f-75f1-b087-4d31c616274c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_019ef11c-c08f-75f1-b087-4d31c616274c" xlink:to="loc_us-gaap_PlanNameDomain_019ef11c-c08f-75f1-b087-4d31c616274c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_019ef11c-c08f-737f-ad42-3ba58c505162" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_019ef11c-c08f-75f1-b087-4d31c616274c" xlink:to="loc_us-gaap_PlanNameDomain_019ef11c-c08f-737f-ad42-3ba58c505162" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_TwoThousandNineteenEquityIncentivePlanMember_019ef11c-c08f-72c3-9fe5-b6612e3e8bc8" xlink:href="real-20260630.xsd#real_TwoThousandNineteenEquityIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_019ef11c-c08f-737f-ad42-3ba58c505162" xlink:to="loc_real_TwoThousandNineteenEquityIncentivePlanMember_019ef11c-c08f-72c3-9fe5-b6612e3e8bc8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_InducementGrantsMember_019ef11c-c08f-7bb6-b3eb-89fb0319a23a" xlink:href="real-20260630.xsd#real_InducementGrantsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_019ef11c-c08f-737f-ad42-3ba58c505162" xlink:to="loc_real_InducementGrantsMember_019ef11c-c08f-7bb6-b3eb-89fb0319a23a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019ef11c-c08f-7b58-afb1-db0ffe6daaa8" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:to="loc_srt_RangeAxis_019ef11c-c08f-7b58-afb1-db0ffe6daaa8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019ef11c-c08f-7b58-afb1-db0ffe6daaa8_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019ef11c-c08f-7b58-afb1-db0ffe6daaa8" xlink:to="loc_srt_RangeMember_019ef11c-c08f-7b58-afb1-db0ffe6daaa8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019ef11c-c08f-7762-a2bf-2f46cf446c6c" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019ef11c-c08f-7b58-afb1-db0ffe6daaa8" xlink:to="loc_srt_RangeMember_019ef11c-c08f-7762-a2bf-2f46cf446c6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019ef11c-c08f-7a28-ac88-debc45d33dfd" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019ef11c-c08f-7762-a2bf-2f46cf446c6c" xlink:to="loc_srt_MinimumMember_019ef11c-c08f-7a28-ac88-debc45d33dfd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019ef11c-c08f-7f35-8730-d481d4fae362" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019ef11c-c08f-7762-a2bf-2f46cf446c6c" xlink:to="loc_srt_MaximumMember_019ef11c-c08f-7f35-8730-d481d4fae362" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusAxis_019ef11c-c08f-7872-9845-ca1900af960e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GranteeStatusAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:to="loc_us-gaap_GranteeStatusAxis_019ef11c-c08f-7872-9845-ca1900af960e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_019ef11c-c08f-7872-9845-ca1900af960e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_GranteeStatusAxis_019ef11c-c08f-7872-9845-ca1900af960e" xlink:to="loc_us-gaap_GranteeStatusDomain_019ef11c-c08f-7872-9845-ca1900af960e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GranteeStatusDomain_019ef11c-c08f-7e59-a01a-56953150fa14" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GranteeStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_GranteeStatusAxis_019ef11c-c08f-7872-9845-ca1900af960e" xlink:to="loc_us-gaap_GranteeStatusDomain_019ef11c-c08f-7e59-a01a-56953150fa14" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_019ef11c-c08f-7001-95e1-d306f5ee2142" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedPaymentArrangementEmployeeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GranteeStatusDomain_019ef11c-c08f-7e59-a01a-56953150fa14" xlink:to="loc_us-gaap_ShareBasedPaymentArrangementEmployeeMember_019ef11c-c08f-7001-95e1-d306f5ee2142" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_019ef11c-c08f-799a-8790-3785b608d3cf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7b79-9976-f1c00860459f" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_019ef11c-c08f-799a-8790-3785b608d3cf" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019ef11c-c08f-799a-8790-3785b608d3cf_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_019ef11c-c08f-799a-8790-3785b608d3cf" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019ef11c-c08f-799a-8790-3785b608d3cf_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019ef11c-c08f-73ca-956f-cf7d55329102" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_019ef11c-c08f-799a-8790-3785b608d3cf" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019ef11c-c08f-73ca-956f-cf7d55329102" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_019ef11c-c08f-760d-b64e-dd4be4a3206f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019ef11c-c08f-73ca-956f-cf7d55329102" xlink:to="loc_us-gaap_EmployeeStockMember_019ef11c-c08f-760d-b64e-dd4be4a3206f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_019ef11c-c08f-7a66-a8e3-592017b18ac8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_019ef11c-c08f-7a66-a8e3-592017b18ac8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorizedAnnualIncreaseShares_019ef11c-c08f-720d-a2d3-97e18d212241" xlink:href="real-20260630.xsd#real_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorizedAnnualIncreaseShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorizedAnnualIncreaseShares_019ef11c-c08f-720d-a2d3-97e18d212241" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorizedAnnualIncreasePercentOfSharesOutstanding_019ef11c-c08f-7a74-b832-214de3189d46" xlink:href="real-20260630.xsd#real_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorizedAnnualIncreasePercentOfSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorizedAnnualIncreasePercentOfSharesOutstanding_019ef11c-c08f-7a74-b832-214de3189d46" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardVestingNumberOfTranche_019ef11c-c08f-7e6e-a2dc-a79d41e73768" xlink:href="real-20260630.xsd#real_ShareBasedCompensationArrangementByShareBasedPaymentAwardVestingNumberOfTranche"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardVestingNumberOfTranche_019ef11c-c08f-7e6e-a2dc-a79d41e73768" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_019ef11c-c08f-7a82-86f7-df40bf4f742f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_019ef11c-c08f-7a82-86f7-df40bf4f742f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardPerformanceTargetPercentage_019ef11c-c08f-713b-b924-4f8786e8c0f5" xlink:href="real-20260630.xsd#real_ShareBasedCompensationArrangementByShareBasedPaymentAwardPerformanceTargetPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_real_ShareBasedCompensationArrangementByShareBasedPaymentAwardPerformanceTargetPercentage_019ef11c-c08f-713b-b924-4f8786e8c0f5" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_019ef11c-c08f-7ad0-b5ba-404388eb4501" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_019ef11c-c08f-7ad0-b5ba-404388eb4501" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_019ef11c-c08f-7c5a-b8cd-efdc36c8c8a1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_019ef11c-c08f-7c5a-b8cd-efdc36c8c8a1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_019ef11c-c08f-74c5-ab41-d4d2100ce78d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_019ef11c-c08f-74c5-ab41-d4d2100ce78d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_019ef11c-c08f-7267-ab2d-5c21478a5c55" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardPurchasePriceOfCommonStockPercent_019ef11c-c08f-7267-ab2d-5c21478a5c55" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesPurchasedForAward_019ef11c-c08f-7b47-b099-ecfc8e648123" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesPurchasedForAward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesPurchasedForAward_019ef11c-c08f-7b47-b099-ecfc8e648123" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount_019f18bf-f49f-7cf5-b4ac-500d54d8e3f4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-79d9-8559-1e26407d1ed9" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount_019f18bf-f49f-7cf5-b4ac-500d54d8e3f4" xlink:type="arc" order="11"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/SharebasedCompensationPlansScheduleofTotalStockbasedCompensationExpenseDetails" xlink:type="simple" xlink:href="real-20260630.xsd#SharebasedCompensationPlansScheduleofTotalStockbasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/SharebasedCompensationPlansScheduleofTotalStockbasedCompensationExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-7b64-bd3e-6be9aa05ddfc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7fa7-be44-e466a6fcb50b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-7b64-bd3e-6be9aa05ddfc" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7fa7-be44-e466a6fcb50b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementOfIncomeLocationBalanceAxis_019ef11c-c08f-741e-8c66-b192aedb31ba" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementOfIncomeLocationBalanceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7fa7-be44-e466a6fcb50b" xlink:to="loc_us-gaap_StatementOfIncomeLocationBalanceAxis_019ef11c-c08f-741e-8c66-b192aedb31ba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_019ef11c-c08f-7f9c-9834-638a1763677b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-7b64-bd3e-6be9aa05ddfc" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_019ef11c-c08f-7f9c-9834-638a1763677b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/SharebasedCompensationPlansScheduleofTotalStockbasedCompensationExpenseDetails_1" xlink:type="simple" xlink:href="real-20260630.xsd#SharebasedCompensationPlansScheduleofTotalStockbasedCompensationExpenseDetails_1"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/SharebasedCompensationPlansScheduleofTotalStockbasedCompensationExpenseDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-7b64-bd3e-6be9aa05ddfc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7fa7-be44-e466a6fcb50b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-7b64-bd3e-6be9aa05ddfc" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019ef11c-c08f-7fa7-be44-e466a6fcb50b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_019ef11c-c08f-7f9c-9834-638a1763677b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019ef11c-c08f-7b64-bd3e-6be9aa05ddfc" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_019ef11c-c08f-7f9c-9834-638a1763677b" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/LeasesAdditionalInformationDetails" xlink:type="simple" xlink:href="real-20260630.xsd#LeasesAdditionalInformationDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/LeasesAdditionalInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_real_LeasesLineItems_019ef11c-c08f-77b9-980d-962a8d813a87" xlink:href="real-20260630.xsd#real_LeasesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_LeasesTable_019ef11c-c08f-79aa-bfa8-a5fddc13ea41" xlink:href="real-20260630.xsd#real_LeasesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_real_LeasesLineItems_019ef11c-c08f-77b9-980d-962a8d813a87" xlink:to="loc_real_LeasesTable_019ef11c-c08f-79aa-bfa8-a5fddc13ea41" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019ef11c-c08f-7bdc-a7b3-32fe11db976f" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_real_LeasesTable_019ef11c-c08f-79aa-bfa8-a5fddc13ea41" xlink:to="loc_srt_RangeAxis_019ef11c-c08f-7bdc-a7b3-32fe11db976f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019ef11c-c08f-7bdc-a7b3-32fe11db976f_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019ef11c-c08f-7bdc-a7b3-32fe11db976f" xlink:to="loc_srt_RangeMember_019ef11c-c08f-7bdc-a7b3-32fe11db976f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019ef11c-c08f-7171-9a1f-513f6ef28983" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019ef11c-c08f-7bdc-a7b3-32fe11db976f" xlink:to="loc_srt_RangeMember_019ef11c-c08f-7171-9a1f-513f6ef28983" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019ef11c-c08f-7341-a901-76a4eeb2d274" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019ef11c-c08f-7171-9a1f-513f6ef28983" xlink:to="loc_srt_MinimumMember_019ef11c-c08f-7341-a901-76a4eeb2d274" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019ef11c-c08f-748c-9eb0-649783f09ec6" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019ef11c-c08f-7171-9a1f-513f6ef28983" xlink:to="loc_srt_MaximumMember_019ef11c-c08f-748c-9eb0-649783f09ec6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseTermOfContract_019ef11c-c08f-7369-9a7c-cb7a61a38ed3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LesseeOperatingLeaseTermOfContract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_LeasesLineItems_019ef11c-c08f-77b9-980d-962a8d813a87" xlink:to="loc_us-gaap_LesseeOperatingLeaseTermOfContract_019ef11c-c08f-7369-9a7c-cb7a61a38ed3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseCost_019ef11c-c08f-776f-ab65-de4687d08b5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_LeasesLineItems_019ef11c-c08f-77b9-980d-962a8d813a87" xlink:to="loc_us-gaap_OperatingLeaseCost_019ef11c-c08f-776f-ab65-de4687d08b5c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableLeaseCost_019ef11c-c08f-7c8b-a663-8161ccfa240e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_VariableLeaseCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_real_LeasesLineItems_019ef11c-c08f-77b9-980d-962a8d813a87" xlink:to="loc_us-gaap_VariableLeaseCost_019ef11c-c08f-7c8b-a663-8161ccfa240e" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/CommitmentsandContingenciesDetails" xlink:type="simple" xlink:href="real-20260630.xsd#CommitmentsandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/CommitmentsandContingenciesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherCommitmentsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCommitmentsTable_019ef11c-c090-76c5-9298-da1d51f7eb2f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherCommitmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_us-gaap_OtherCommitmentsTable_019ef11c-c090-76c5-9298-da1d51f7eb2f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseAxis_019ef11c-c090-791e-ad59-35d2677246b0" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_LitigationCaseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OtherCommitmentsTable_019ef11c-c090-76c5-9298-da1d51f7eb2f" xlink:to="loc_srt_LitigationCaseAxis_019ef11c-c090-791e-ad59-35d2677246b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_019ef11c-c090-791e-ad59-35d2677246b0_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_LitigationCaseAxis_019ef11c-c090-791e-ad59-35d2677246b0" xlink:to="loc_srt_LitigationCaseTypeDomain_019ef11c-c090-791e-ad59-35d2677246b0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_LitigationCaseTypeDomain_019ef11c-c090-725c-9e1e-e1f33e42cf80" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_LitigationCaseTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_LitigationCaseAxis_019ef11c-c090-791e-ad59-35d2677246b0" xlink:to="loc_srt_LitigationCaseTypeDomain_019ef11c-c090-725c-9e1e-e1f33e42cf80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ClassActionsComplaintFiledInSanMateoCountyCaliforniaMember_019ef11c-c090-7e63-a6e6-bb642fed46d1" xlink:href="real-20260630.xsd#real_ClassActionsComplaintFiledInSanMateoCountyCaliforniaMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_LitigationCaseTypeDomain_019ef11c-c090-725c-9e1e-e1f33e42cf80" xlink:to="loc_real_ClassActionsComplaintFiledInSanMateoCountyCaliforniaMember_019ef11c-c090-7e63-a6e6-bb642fed46d1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermPurchaseCommitmentAmount_019f18d1-a50a-7020-acf1-96173b6386ed" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermPurchaseCommitmentAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_us-gaap_LongTermPurchaseCommitmentAmount_019f18d1-a50a-7020-acf1-96173b6386ed" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermPurchaseCommitmentPeriod_019f18d1-a50a-705a-a66a-0a92219f9463" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermPurchaseCommitmentPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_us-gaap_LongtermPurchaseCommitmentPeriod_019f18d1-a50a-705a-a66a-0a92219f9463" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationSettlementAmountAwardedToOtherParty_019f18d1-cc0b-723a-83dc-37559972289e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LitigationSettlementAmountAwardedToOtherParty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_us-gaap_LitigationSettlementAmountAwardedToOtherParty_019f18d1-cc0b-723a-83dc-37559972289e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_LitigationSettlementPaymentPeriodForAmountAwardedToOtherParty_019f18d1-cc0b-7c13-b501-1a3c63bd4a9d" xlink:href="real-20260630.xsd#real_LitigationSettlementPaymentPeriodForAmountAwardedToOtherParty"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_real_LitigationSettlementPaymentPeriodForAmountAwardedToOtherParty_019f18d1-cc0b-7c13-b501-1a3c63bd4a9d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LitigationSettlementExpense_019f18d1-cc0b-74d5-a751-17ef0fe401db" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LitigationSettlementExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_us-gaap_LitigationSettlementExpense_019f18d1-cc0b-74d5-a751-17ef0fe401db" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_LossContingencyNumberOfPlaintiffsOptingOut_019f18d1-cc0b-7982-91ef-fdccfa42c2d6" xlink:href="real-20260630.xsd#real_LossContingencyNumberOfPlaintiffsOptingOut"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_real_LossContingencyNumberOfPlaintiffsOptingOut_019f18d1-cc0b-7982-91ef-fdccfa42c2d6" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_LossContingencyNumberOfPlaintiffsOptingOutOrPurchasedStock_019f18d1-cc0b-700b-814e-4b7f51666636" xlink:href="real-20260630.xsd#real_LossContingencyNumberOfPlaintiffsOptingOutOrPurchasedStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherCommitmentsLineItems_019ef11c-c090-7795-b591-41a870653382" xlink:to="loc_real_LossContingencyNumberOfPlaintiffsOptingOutOrPurchasedStock_019f18d1-cc0b-700b-814e-4b7f51666636" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.therealreal.com/role/NetIncomeLossPerShareAttributabletoCommonStockholdersScheduleofAntidilutiveSecuritiesExcludedfromComputationofDilutedNetLossPerShareDetails" xlink:type="simple" xlink:href="real-20260630.xsd#NetIncomeLossPerShareAttributabletoCommonStockholdersScheduleofAntidilutiveSecuritiesExcludedfromComputationofDilutedNetLossPerShareDetails"/>
  <link:definitionLink xlink:role="http://www.therealreal.com/role/NetIncomeLossPerShareAttributabletoCommonStockholdersScheduleofAntidilutiveSecuritiesExcludedfromComputationofDilutedNetLossPerShareDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_019ef11c-c090-70b8-82be-a2977ef6c061" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_019ef11c-c090-7fe4-a329-b585b241280b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_019ef11c-c090-70b8-82be-a2977ef6c061" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_019ef11c-c090-7fe4-a329-b585b241280b" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019ef11c-c090-725d-b018-adfb0e46c3e5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_019ef11c-c090-7fe4-a329-b585b241280b" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019ef11c-c090-725d-b018-adfb0e46c3e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-725d-b018-adfb0e46c3e5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019ef11c-c090-725d-b018-adfb0e46c3e5" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-725d-b018-adfb0e46c3e5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019ef11c-c090-725d-b018-adfb0e46c3e5" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_019ef11c-c090-7187-a462-5c820e4fd338" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:to="loc_us-gaap_EmployeeStockOptionMember_019ef11c-c090-7187-a462-5c820e4fd338" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019ef11c-c090-7ee8-aadf-6f6cf4be6b49" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019ef11c-c090-7ee8-aadf-6f6cf4be6b49" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_019ef11c-c090-7376-ac00-f34e11ee4ab2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:to="loc_us-gaap_EmployeeStockMember_019ef11c-c090-7376-ac00-f34e11ee4ab2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_real_ConvertibleSeniorNotesMember_019ef11c-c090-7b81-ae3f-0478e77148f3" xlink:href="real-20260630.xsd#real_ConvertibleSeniorNotesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:to="loc_real_ConvertibleSeniorNotesMember_019ef11c-c090-7b81-ae3f-0478e77148f3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WarrantMember_019ef11c-c090-71bb-991d-44f409b3cff4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019ef11c-c090-73f6-9968-449d7b43ad93" xlink:to="loc_us-gaap_WarrantMember_019ef11c-c090-71bb-991d-44f409b3cff4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019ef11c-c090-723d-88d5-87baeb4e573d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_019ef11c-c090-70b8-82be-a2977ef6c061" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019ef11c-c090-723d-88d5-87baeb4e573d" xlink:type="arc" order="0"/>
  </link:definitionLink>
</link:linkbase>
