<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019df4d8-0d54-71e6-a093-e931a649c7af,g:1a97f33f-2ba6-4d4d-b2a9-637a25e3254e-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.evolus.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="simple" xlink:href="eols-20260630.xsd#CondensedConsolidatedStatementsofOperationsandComprehensiveLoss"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019df4d8-0e1a-7bad-a942-3f2f74d24086" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_StatementTable_019df4d8-0e1a-7bad-a942-3f2f74d24086" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_019df4d8-0e1a-73c5-a184-f76cd7188f24" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019df4d8-0e1a-7bad-a942-3f2f74d24086" xlink:to="loc_srt_ProductOrServiceAxis_019df4d8-0e1a-73c5-a184-f76cd7188f24" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019df4d8-0e1a-73c5-a184-f76cd7188f24_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_019df4d8-0e1a-73c5-a184-f76cd7188f24" xlink:to="loc_srt_ProductsAndServicesDomain_019df4d8-0e1a-73c5-a184-f76cd7188f24_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019df4d8-0e1a-7e7a-aedb-3d05259f982b" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_019df4d8-0e1a-73c5-a184-f76cd7188f24" xlink:to="loc_srt_ProductsAndServicesDomain_019df4d8-0e1a-7e7a-aedb-3d05259f982b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_019df4d8-0e1a-7f46-8242-b2fd02c2dee2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019df4d8-0e1a-7e7a-aedb-3d05259f982b" xlink:to="loc_us-gaap_ProductMember_019df4d8-0e1a-7f46-8242-b2fd02c2dee2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServiceMember_019df4d8-0e1a-7d86-bafd-0cf649f94fdb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019df4d8-0e1a-7e7a-aedb-3d05259f982b" xlink:to="loc_us-gaap_ServiceMember_019df4d8-0e1a-7d86-bafd-0cf649f94fdb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_019df4d8-0e1a-7c8c-b5d4-a8c5d10c764a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_RevenuesAbstract_019df4d8-0e1a-7c8c-b5d4-a8c5d10c764a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019df4d8-0e1a-79d7-887d-ea52324ec8d1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_019df4d8-0e1a-7c8c-b5d4-a8c5d10c764a" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019df4d8-0e1a-79d7-887d-ea52324ec8d1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_019df4d8-0e1a-77c4-a2e6-93c3c92bbfaa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_019df4d8-0e1a-77c4-a2e6-93c3c92bbfaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfCostGoodOrServiceExtensibleList_019df4d8-0e1a-7c6d-8c2d-70a0c32b82ff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfCostGoodOrServiceExtensibleList"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostOfGoodsAndServicesSold_019df4d8-0e1a-77c4-a2e6-93c3c92bbfaa" xlink:to="loc_us-gaap_TypeOfCostGoodOrServiceExtensibleList_019df4d8-0e1a-7c6d-8c2d-70a0c32b82ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_019df4d8-0e1a-763c-8c04-9e5355073f05" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_GrossProfit_019df4d8-0e1a-763c-8c04-9e5355073f05" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0e1a-7f2a-a58e-1a4ac8925d6c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0e1a-7f2a-a58e-1a4ac8925d6c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseExcludingAcquiredInProcessCost_019df4d8-0e1a-7787-a0ca-7c55f0789667" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResearchAndDevelopmentExpenseExcludingAcquiredInProcessCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseExcludingAcquiredInProcessCost_019df4d8-0e1a-7787-a0ca-7c55f0789667" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilityGainLossIncludedInEarnings_019df4d8-0e1a-7c9b-a109-a03b5bbd62ff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilityGainLossIncludedInEarnings"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationRecurringBasisLiabilityGainLossIncludedInEarnings_019df4d8-0e1a-7c9b-a109-a03b5bbd62ff" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_019df4d8-0e1a-7c4b-a454-e24ad6e55884" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:to="loc_us-gaap_DepreciationAndAmortization_019df4d8-0e1a-7c4b-a454-e24ad6e55884" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_019df4d8-0e1a-7813-b869-763cbc492a53" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostsAndExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_019df4d8-0e1a-7693-a5e2-147b3ff332c0" xlink:to="loc_us-gaap_CostsAndExpenses_019df4d8-0e1a-7813-b869-763cbc492a53" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019df4d8-0e1a-79a8-92cb-20c6768c06fa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_OperatingIncomeLoss_019df4d8-0e1a-79a8-92cb-20c6768c06fa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpenseAbstract_019df4d8-0e1a-7e58-92d5-5e2e184ada8c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherNonoperatingIncomeExpenseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpenseAbstract_019df4d8-0e1a-7e58-92d5-5e2e184ada8c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeNet_019df4d8-0e1a-726c-8f77-afacd8ffcd80" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentIncomeNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpenseAbstract_019df4d8-0e1a-7e58-92d5-5e2e184ada8c" xlink:to="loc_us-gaap_InvestmentIncomeNet_019df4d8-0e1a-726c-8f77-afacd8ffcd80" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_019df4d8-0e1a-7a7f-b278-b70b01c5c188" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpenseAbstract_019df4d8-0e1a-7e58-92d5-5e2e184ada8c" xlink:to="loc_us-gaap_InterestExpenseNonoperating_019df4d8-0e1a-7a7f-b278-b70b01c5c188" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_019df4d8-0e1a-7812-8b38-a0c4e777f0b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpenseAbstract_019df4d8-0e1a-7e58-92d5-5e2e184ada8c" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_019df4d8-0e1a-7812-8b38-a0c4e777f0b8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019df4d8-0e1a-78a6-868b-c474cb59485d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019df4d8-0e1a-78a6-868b-c474cb59485d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0e1a-75c5-be80-5a2ba8c5a63d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0e1a-75c5-be80-5a2ba8c5a63d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019df4d8-0e1a-7178-a3f9-ba7d8a0f01bf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_NetIncomeLoss_019df4d8-0e1a-7178-a3f9-ba7d8a0f01bf" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_019df4d8-0e1a-7447-9429-47c951b5fe2b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_019df4d8-0e1a-7447-9429-47c951b5fe2b" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_019df4d8-0e1a-77da-b878-7c48d9807881" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPeriodIncreaseDecreaseAbstract_019df4d8-0e1a-7447-9429-47c951b5fe2b" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossForeignCurrencyTransactionAndTranslationAdjustmentNetOfTax_019df4d8-0e1a-77da-b878-7c48d9807881" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_019df4d8-0e1a-7f2b-a6a5-2d88e496d74a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_ComprehensiveIncomeNetOfTax_019df4d8-0e1a-7f2b-a6a5-2d88e496d74a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_019df4d8-0e1a-774d-ae5c-ed5d50f07ca1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_EarningsPerShareBasic_019df4d8-0e1a-774d-ae5c-ed5d50f07ca1" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareDiluted_019df4d8-0e1a-7d6e-8361-64b358981256" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EarningsPerShareDiluted"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_EarningsPerShareDiluted_019df4d8-0e1a-7d6e-8361-64b358981256" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019df4d8-0e1a-78cc-bac1-85ac66fb7d28" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019df4d8-0e1a-78cc-bac1-85ac66fb7d28" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019df4d8-0e1a-7ecc-95a7-ce498cf3fdf3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7448-bcdf-6e87a8b061e9" xlink:to="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019df4d8-0e1a-7ecc-95a7-ce498cf3fdf3" xlink:type="arc" order="14"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/CondensedConsolidatedStatementsofStockholdersEquityDeficit" xlink:type="simple" xlink:href="eols-20260630.xsd#CondensedConsolidatedStatementsofStockholdersEquityDeficit"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/CondensedConsolidatedStatementsofStockholdersEquityDeficit" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7ba7-b32c-4cd62fd59795" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_019df4d8-0e1a-70bf-8b19-b95e6d06d43f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7ba7-b32c-4cd62fd59795" xlink:to="loc_us-gaap_StatementTable_019df4d8-0e1a-70bf-8b19-b95e6d06d43f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-77c2-a47c-4f94b8f02529" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_019df4d8-0e1a-70bf-8b19-b95e6d06d43f" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-77c2-a47c-4f94b8f02529" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-77c2-a47c-4f94b8f02529_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-77c2-a47c-4f94b8f02529" xlink:to="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-77c2-a47c-4f94b8f02529_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7731-aa6e-3d974be12097" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-77c2-a47c-4f94b8f02529" xlink:to="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7731-aa6e-3d974be12097" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_019df4d8-0e1a-7a50-8657-c1a28e0e0d5a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7731-aa6e-3d974be12097" xlink:to="loc_us-gaap_CommonStockMember_019df4d8-0e1a-7a50-8657-c1a28e0e0d5a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_019df4d8-0e1a-7fbc-b99a-ee2cf9773310" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7731-aa6e-3d974be12097" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_019df4d8-0e1a-7fbc-b99a-ee2cf9773310" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019df4d8-0e1a-7983-addf-a0ba1b9d9751" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7731-aa6e-3d974be12097" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeMember_019df4d8-0e1a-7983-addf-a0ba1b9d9751" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_019df4d8-0e1a-7016-9c21-7fc7aa13ef7b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7731-aa6e-3d974be12097" xlink:to="loc_us-gaap_RetainedEarningsMember_019df4d8-0e1a-7016-9c21-7fc7aa13ef7b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_019df4d8-0e1a-7ba7-b32c-4cd62fd59795" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_019df4d8-0e1a-7792-9134-0b40ec561e97" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_us-gaap_CommonStockSharesIssued_019df4d8-0e1a-7792-9134-0b40ec561e97" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019df4d8-0e1a-708d-b3b6-b81f68943127" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_us-gaap_StockholdersEquity_019df4d8-0e1a-708d-b3b6-b81f68943127" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_StockIssuedDuringPeriodSharesIncentiveEquityPlan_019df4d8-0e1a-76e5-8358-ed9bcb2aeae7" xlink:href="eols-20260630.xsd#eols_StockIssuedDuringPeriodSharesIncentiveEquityPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_eols_StockIssuedDuringPeriodSharesIncentiveEquityPlan_019df4d8-0e1a-76e5-8358-ed9bcb2aeae7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_StockIssuedDuringPeriodValueIncentiveEquityPlan_019df4d8-0e1a-74ff-889f-a995e8b553e0" xlink:href="eols-20260630.xsd#eols_StockIssuedDuringPeriodValueIncentiveEquityPlan"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_eols_StockIssuedDuringPeriodValueIncentiveEquityPlan_019df4d8-0e1a-74ff-889f-a995e8b553e0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_019df4d8-0e1a-72a1-938c-58bb119a4f5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_019df4d8-0e1a-72a1-938c-58bb119a4f5c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019df4d8-0e1a-7dce-a46b-32663574c48e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_us-gaap_NetIncomeLoss_019df4d8-0e1a-7dce-a46b-32663574c48e" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_019df4d8-0e1a-78ef-94f2-452e5008e08d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_019df4d8-0e1a-74fb-aacd-a3493ca4be12" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_019df4d8-0e1a-78ef-94f2-452e5008e08d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_019df4d8-0e1a-770f-b8c0-b35438defaa7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019df4d8-0e1a-722e-b46a-15f63523d6fa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/DescriptionofBusinessDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#DescriptionofBusinessDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/DescriptionofBusinessDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsidiarySaleOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7f3f-9c59-1befeb9b845e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:to="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7f3f-9c59-1befeb9b845e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7f3f-9c59-1befeb9b845e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7f3f-9c59-1befeb9b845e" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7f3f-9c59-1befeb9b845e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-705e-8a53-18b6643339be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7f3f-9c59-1befeb9b845e" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-705e-8a53-18b6643339be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseU.S.AgreementMember_019df4d8-0e1a-7c87-a6f9-4d6b0ea5e086" xlink:href="eols-20260630.xsd#eols_SymateseU.S.AgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-705e-8a53-18b6643339be" xlink:to="loc_eols_SymateseU.S.AgreementMember_019df4d8-0e1a-7c87-a6f9-4d6b0ea5e086" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseEuropeAgreementMember_019df4d8-0e1a-7977-9180-895895244d18" xlink:href="eols-20260630.xsd#eols_SymateseEuropeAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-705e-8a53-18b6643339be" xlink:to="loc_eols_SymateseEuropeAgreementMember_019df4d8-0e1a-7977-9180-895895244d18" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019df4d8-0e1a-721c-b3e4-c5946997b746" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:to="loc_us-gaap_DebtInstrumentAxis_019df4d8-0e1a-721c-b3e4-c5946997b746" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-721c-b3e4-c5946997b746_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0e1a-721c-b3e4-c5946997b746" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-721c-b3e4-c5946997b746_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0e1a-721c-b3e4-c5946997b746" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementMember_019df4d8-0e1a-7f4d-8674-fae9eb51ceb3" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:to="loc_eols_ARLoanAgreementMember_019df4d8-0e1a-7f4d-8674-fae9eb51ceb3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheOneMember_019df4d8-0e1a-7b3d-a984-cdb98b8fa833" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:to="loc_eols_ARLoanAgreementTrancheOneMember_019df4d8-0e1a-7b3d-a984-cdb98b8fa833" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheTwoMember_019df4d8-0e1a-7159-9577-2038d0d23277" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:to="loc_eols_ARLoanAgreementTrancheTwoMember_019df4d8-0e1a-7159-9577-2038d0d23277" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheThreeMember_019df4d8-0e1a-71a9-9be1-abf7cc562cdd" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:to="loc_eols_ARLoanAgreementTrancheThreeMember_019df4d8-0e1a-71a9-9be1-abf7cc562cdd" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LoanAgreementMember_019df4d8-0e1a-7177-8d36-114cf7fb3147" xlink:href="eols-20260630.xsd#eols_LoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0e1a-7647-811d-c7ab5940a194" xlink:to="loc_eols_LoanAgreementMember_019df4d8-0e1a-7177-8d36-114cf7fb3147" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0e1a-713c-af62-6457a7ac5a26" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0e1a-713c-af62-6457a7ac5a26" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0e1a-713c-af62-6457a7ac5a26_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0e1a-713c-af62-6457a7ac5a26" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0e1a-713c-af62-6457a7ac5a26_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0e1a-7988-abfd-5823031be524" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0e1a-713c-af62-6457a7ac5a26" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0e1a-7988-abfd-5823031be524" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_019df4d8-0e1a-7f5a-bdde-463b9f95f2e1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0e1a-7988-abfd-5823031be524" xlink:to="loc_us-gaap_LineOfCreditMember_019df4d8-0e1a-7f5a-bdde-463b9f95f2e1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_019df4d8-0e1a-7c58-8314-3dd9a04b31f0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:to="loc_us-gaap_CreditFacilityAxis_019df4d8-0e1a-7c58-8314-3dd9a04b31f0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019df4d8-0e1a-7c58-8314-3dd9a04b31f0_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_019df4d8-0e1a-7c58-8314-3dd9a04b31f0" xlink:to="loc_us-gaap_CreditFacilityDomain_019df4d8-0e1a-7c58-8314-3dd9a04b31f0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019df4d8-0e1a-7ca4-88f3-41bd55c0dc8b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_019df4d8-0e1a-7c58-8314-3dd9a04b31f0" xlink:to="loc_us-gaap_CreditFacilityDomain_019df4d8-0e1a-7ca4-88f3-41bd55c0dc8b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_019df4d8-0e1a-72b4-a793-f71bb3aa5015" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019df4d8-0e1a-7ca4-88f3-41bd55c0dc8b" xlink:to="loc_us-gaap_SecuredDebtMember_019df4d8-0e1a-72b4-a793-f71bb3aa5015" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_019df4d8-0e1a-78b4-b60b-7d1e315641af" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019df4d8-0e1a-7ca4-88f3-41bd55c0dc8b" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_019df4d8-0e1a-78b4-b60b-7d1e315641af" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0e1a-73d9-b313-cb580082397f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsidiaryOrEquityMethodInvesteeSaleOfStockBySubsidiaryOrEquityInvesteeTable_019df4d8-0e1a-7890-afeb-f06934bb4fa8" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0e1a-73d9-b313-cb580082397f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0e1a-73d9-b313-cb580082397f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0e1a-73d9-b313-cb580082397f" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0e1a-73d9-b313-cb580082397f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0e1a-712d-843f-f748e5081fff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0e1a-73d9-b313-cb580082397f" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0e1a-712d-843f-f748e5081fff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ATMSalesAgreementMember_019df4d8-0e1a-7b07-a607-0d6ce42daf74" xlink:href="eols-20260630.xsd#eols_ATMSalesAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0e1a-712d-843f-f748e5081fff" xlink:to="loc_eols_ATMSalesAgreementMember_019df4d8-0e1a-7b07-a607-0d6ce42daf74" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementExpectedRegulatoryApprovalNumberOfProducts_019df4d8-0e1a-7417-94f9-af78340d30fe" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementExpectedRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementExpectedRegulatoryApprovalNumberOfProducts_019df4d8-0e1a-7417-94f9-af78340d30fe" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f6eb3-85b2-7d68-9d52-ac5c2aad9a1f" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f6eb3-85b2-7d68-9d52-ac5c2aad9a1f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019df4d8-0e1a-7534-bee4-b3ab063d576c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_OperatingIncomeLoss_019df4d8-0e1a-7534-bee4-b3ab063d576c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019df4d8-0e1a-7442-83cc-dfb60f00f609" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_NetIncomeLoss_019df4d8-0e1a-7442-83cc-dfb60f00f609" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019df4d8-0e1a-72f3-89a9-0e21eea78b2c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019df4d8-0e1a-72f3-89a9-0e21eea78b2c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019df4d8-0e1a-7649-8c2b-263f93913f72" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019df4d8-0e1a-7649-8c2b-263f93913f72" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_019df4d8-0e1a-701f-b10c-ab434a5eca97" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_019df4d8-0e1a-701f-b10c-ab434a5eca97" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_019df4d8-0e1a-7610-86f2-e5c35be95476" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_019df4d8-0e1a-7610-86f2-e5c35be95476" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityAccordionFeatureMaximumLimit_019df4d8-0e1a-7a4d-bd88-1c75e9f203de" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityAccordionFeatureMaximumLimit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_LineOfCreditFacilityAccordionFeatureMaximumLimit_019df4d8-0e1a-7a4d-bd88-1c75e9f203de" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityAccordionFeatureElectableIncrementAmount_019df4d8-0e1a-7b49-ab06-3b71271e3222" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityAccordionFeatureElectableIncrementAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_LineOfCreditFacilityAccordionFeatureElectableIncrementAmount_019df4d8-0e1a-7b49-ab06-3b71271e3222" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityMinimumUtilizationThresholdAmount_019df4d8-0e1a-7daa-8241-62a622a7e9b5" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityMinimumUtilizationThresholdAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_LineOfCreditFacilityMinimumUtilizationThresholdAmount_019df4d8-0e1a-7daa-8241-62a622a7e9b5" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentNumberOfTranches_019df4d8-0e1a-7e7b-bce2-2e55a2435a41" xlink:href="eols-20260630.xsd#eols_DebtInstrumentNumberOfTranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_DebtInstrumentNumberOfTranches_019df4d8-0e1a-7e7b-bce2-2e55a2435a41" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSecuredLinesOfCredit_019df4d8-0e1a-745d-80f0-9e051ee08113" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromSecuredLinesOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_ProceedsFromSecuredLinesOfCredit_019df4d8-0e1a-745d-80f0-9e051ee08113" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityMaximumBorrowingCapacityOption_019df4d8-0e1a-7921-b37a-9771c32b2355" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityMaximumBorrowingCapacityOption"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_LineOfCreditFacilityMaximumBorrowingCapacityOption_019df4d8-0e1a-7921-b37a-9771c32b2355" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SaleOfStockMaximumConsiderationReceivable_019df4d8-0e1a-71a6-8e2b-28a2bb2be6a9" xlink:href="eols-20260630.xsd#eols_SaleOfStockMaximumConsiderationReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_eols_SaleOfStockMaximumConsiderationReceivable_019df4d8-0e1a-71a6-8e2b-28a2bb2be6a9" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_019df4d8-0e1a-75dd-a35c-28b627a97428" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsidiarySaleOfStockLineItems_019df4d8-0e1a-7231-bf54-8266b4bf2b59" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_019df4d8-0e1a-75dd-a35c-28b627a97428" xlink:type="arc" order="15"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0e1a-7ef7-9459-c504796646d3_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:to="loc_srt_RangeMember_019df4d8-0e1a-7ef7-9459-c504796646d3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:to="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019df4d8-0e1a-74a1-b0ea-31f2052d099e" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:to="loc_srt_MinimumMember_019df4d8-0e1a-74a1-b0ea-31f2052d099e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019df4d8-0e1a-7078-a189-d0c04f219d5e" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:to="loc_srt_MaximumMember_019df4d8-0e1a-7078-a189-d0c04f219d5e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-725d-8430-a708634c81dd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-725d-8430-a708634c81dd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_019df4d8-0e1a-7eb0-a236-1e421e79e8d8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:to="loc_us-gaap_ComputerEquipmentMember_019df4d8-0e1a-7eb0-a236-1e421e79e8d8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_019df4d8-0e1a-7b08-b934-8497ee642f39" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:to="loc_us-gaap_EquipmentMember_019df4d8-0e1a-7b08-b934-8497ee642f39" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_019df4d8-0e1a-73c3-b493-b97471b87d71" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_019df4d8-0e1a-73c3-b493-b97471b87d71" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7882-acdd-cdfb2208125e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7882-acdd-cdfb2208125e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseU.S.AgreementMember_019df4d8-0e1a-7525-8cf7-30b19b531f4c" xlink:href="eols-20260630.xsd#eols_SymateseU.S.AgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:to="loc_eols_SymateseU.S.AgreementMember_019df4d8-0e1a-7525-8cf7-30b19b531f4c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseEuropeAgreementMember_019df4d8-0e1a-7778-8df8-20178f7afa2b" xlink:href="eols-20260630.xsd#eols_SymateseEuropeAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:to="loc_eols_SymateseEuropeAgreementMember_019df4d8-0e1a-7778-8df8-20178f7afa2b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:to="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-71cb-b907-0b7d2ecdd30b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:to="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-71cb-b907-0b7d2ecdd30b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_019df4d8-0e1a-7fa1-93de-c2835d84c213" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-71cb-b907-0b7d2ecdd30b" xlink:to="loc_us-gaap_CommonStockMember_019df4d8-0e1a-7fa1-93de-c2835d84c213" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-7cac-a488-ea9bb8468d8f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-7cac-a488-ea9bb8468d8f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DistributionRightsEvolysseNasolabialFoldProductMember_019df4d8-0e1a-787a-a50b-529d521076d9" xlink:href="eols-20260630.xsd#eols_DistributionRightsEvolysseNasolabialFoldProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_eols_DistributionRightsEvolysseNasolabialFoldProductMember_019df4d8-0e1a-787a-a50b-529d521076d9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DistributionRightsMember_019df4d8-0e1a-72b3-9f44-549ae7ecdd02" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DistributionRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_us-gaap_DistributionRightsMember_019df4d8-0e1a-72b3-9f44-549ae7ecdd02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DistributionRightsEvolysseProductsMember_019df4d8-0e1a-7b37-bfaa-70d0c60e66d3" xlink:href="eols-20260630.xsd#eols_DistributionRightsEvolysseProductsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_eols_DistributionRightsEvolysseProductsMember_019df4d8-0e1a-7b37-bfaa-70d0c60e66d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7718-91f4-1f112a5feeab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SoftwareDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7718-91f4-1f112a5feeab" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:href="eols-20260630.xsd#eols_MilestoneAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneDomain_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f_default" xlink:href="eols-20260630.xsd#eols_MilestoneDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:to="loc_eols_MilestoneDomain_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:href="eols-20260630.xsd#eols_MilestoneDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:to="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_FirstMilestonePaymentMember_019df4d8-0ed6-7044-9557-7b2dee11c852" xlink:href="eols-20260630.xsd#eols_FirstMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:to="loc_eols_FirstMilestonePaymentMember_019df4d8-0ed6-7044-9557-7b2dee11c852" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SecondMilestonePaymentMember_019df4d8-0ed6-7061-9d90-1370ee32fed2" xlink:href="eols-20260630.xsd#eols_SecondMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:to="loc_eols_SecondMilestonePaymentMember_019df4d8-0ed6-7061-9d90-1370ee32fed2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-7a6e-b6ad-055a92a9f420_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-7a6e-b6ad-055a92a9f420_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-73a7-a2ea-9682f236e062" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-73a7-a2ea-9682f236e062" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_EvolusIncMember_019df4d8-0ed6-7375-a4ea-a1132884909b" xlink:href="eols-20260630.xsd#eols_EvolusIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-73a7-a2ea-9682f236e062" xlink:to="loc_eols_EvolusIncMember_019df4d8-0ed6-7375-a4ea-a1132884909b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff_default" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:to="loc_dei_EntityDomain_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019df4d8-0ed6-7a53-b5e5-a7dd95f755a6" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:to="loc_dei_EntityDomain_019df4d8-0ed6-7a53-b5e5-a7dd95f755a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SCHAEONLLCMember_019df4d8-0ed6-7ad4-8f69-5bf04ff7683f" xlink:href="eols-20260630.xsd#eols_SCHAEONLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019df4d8-0ed6-7a53-b5e5-a7dd95f755a6" xlink:to="loc_eols_SCHAEONLLCMember_019df4d8-0ed6-7ad4-8f69-5bf04ff7683f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-756f-ac19-96a08da7132c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-756f-ac19-96a08da7132c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PerformanceRestrictedStockUnitsPRSUMember_019df4d8-0ed6-7d6d-8c52-94bc7c71c2ee" xlink:href="eols-20260630.xsd#eols_PerformanceRestrictedStockUnitsPRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-756f-ac19-96a08da7132c" xlink:to="loc_eols_PerformanceRestrictedStockUnitsPRSUMember_019df4d8-0ed6-7d6d-8c52-94bc7c71c2ee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-74d0-8b6b-81bee167939a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-74d0-8b6b-81bee167939a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_019df4d8-0ed6-7cf7-a554-b5a0b4171c87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:to="loc_us-gaap_EmployeeStockOptionMember_019df4d8-0ed6-7cf7-a554-b5a0b4171c87" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019df4d8-0ed6-7144-973e-9a1d265cceaa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019df4d8-0ed6-7144-973e-9a1d265cceaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementOfIncomeLocationBalanceAxis_019f8d03-fe18-7be3-bfeb-61a63d3e8561" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementOfIncomeLocationBalanceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_StatementOfIncomeLocationBalanceAxis_019f8d03-fe18-7be3-bfeb-61a63d3e8561" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingenciesByNatureOfContingencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-7376-8b52-a82c52f1314c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:to="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-7376-8b52-a82c52f1314c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-75b0-ac35-b63e41fdfda0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:to="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-75b0-ac35-b63e41fdfda0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_IEEPATariffRefundMember_019f8d03-3f79-7ccc-8949-528a61c975df" xlink:href="eols-20260630.xsd#eols_IEEPATariffRefundMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-75b0-ac35-b63e41fdfda0" xlink:to="loc_eols_IEEPATariffRefundMember_019f8d03-3f79-7ccc-8949-528a61c975df" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f6eb5-5f06-704f-a509-01dcff861a21" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f6eb5-5f06-704f-a509-01dcff861a21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_019df4d8-0ed6-7abb-8704-b92e2ee691c0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_NumberOfReportableSegments_019df4d8-0ed6-7abb-8704-b92e2ee691c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_019df4d8-0ed6-7ace-83c8-7d40c403d49b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_NumberOfOperatingSegments_019df4d8-0ed6-7ace-83c8-7d40c403d49b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_AccountsReceivableCreditCardTransactionConvertedToCashPeriod_019df4d8-0ed6-7488-baa0-20d6e7c9701a" xlink:href="eols-20260630.xsd#eols_AccountsReceivableCreditCardTransactionConvertedToCashPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_AccountsReceivableCreditCardTransactionConvertedToCashPeriod_019df4d8-0ed6-7488-baa0-20d6e7c9701a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivableForRecoveryOfImportDutiesNet_019f6e9e-a7d0-7e2d-9b6b-aa4ff3a63f1d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ReceivableForRecoveryOfImportDutiesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ReceivableForRecoveryOfImportDutiesNet_019f6e9e-a7d0-7e2d-9b6b-aa4ff3a63f1d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RecoveryOfDirectCosts_019f6e9e-a7d0-7d52-a6fd-340c32bd3af2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RecoveryOfDirectCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_RecoveryOfDirectCosts_019f6e9e-a7d0-7d52-a6fd-340c32bd3af2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_IEEPATariffRefundOperatingOffset_019fcedb-e7f2-7719-95b3-63bae42c4a9a" xlink:href="eols-20260630.xsd#eols_IEEPATariffRefundOperatingOffset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_IEEPATariffRefundOperatingOffset_019fcedb-e7f2-7719-95b3-63bae42c4a9a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryAdjustments_019f6e9e-a7d0-7b0c-b85a-1af3b968994d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InventoryAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_InventoryAdjustments_019f6e9e-a7d0-7b0c-b85a-1af3b968994d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_019df4d8-0ed6-7da6-b234-53d9446ed20d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_019df4d8-0ed6-7da6-b234-53d9446ed20d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportingUnits_019df4d8-0ed6-765e-8673-bf78684de972" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportingUnits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_NumberOfReportingUnits_019df4d8-0ed6-765e-8673-bf78684de972" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillImpairmentLoss_019df4d8-0ed6-739b-b288-7c2ace21f8e0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GoodwillImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_GoodwillImpairmentLoss_019df4d8-0ed6-739b-b288-7c2ace21f8e0" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment_019df4d8-0ed6-78cd-a95c-e62f70a17e6e" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment_019df4d8-0ed6-78cd-a95c-e62f70a17e6e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement_019df4d8-0ed6-7e37-93cc-b1f3d627276b" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement_019df4d8-0ed6-7e37-93cc-b1f3d627276b" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod_019df4d8-0ed6-754c-ae88-96ffeb523a3a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod_019df4d8-0ed6-754c-ae88-96ffeb523a3a" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne_019df4d8-0ed6-74ac-9239-f4f1435fb783" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne_019df4d8-0ed6-74ac-9239-f4f1435fb783" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo_019df4d8-0ed6-7978-a924-f4ee5d80a442" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo_019df4d8-0ed6-7978-a924-f4ee5d80a442" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree_019df4d8-0ed6-7e4e-a966-860caa505852" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree_019df4d8-0ed6-7e4e-a966-860caa505852" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour_019df4d8-0ed6-7d8b-919f-7ee215d9c265" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour_019df4d8-0ed6-7d8b-919f-7ee215d9c265" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7702-8e4d-f64fe3a20173" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7702-8e4d-f64fe3a20173" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense_019df4d8-0ed6-7368-84ee-9bfff4696192" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense_019df4d8-0ed6-7368-84ee-9bfff4696192" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts_019df4d8-0ed6-7a0e-9ca9-376a4edcb08f" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts_019df4d8-0ed6-7a0e-9ca9-376a4edcb08f" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment_019df4d8-0ed6-76a1-bcf5-9dd82436fd96" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment_019df4d8-0ed6-76a1-bcf5-9dd82436fd96" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialTerm_019df4d8-0ed6-71e0-be60-304369d6591a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialTerm_019df4d8-0ed6-71e0-be60-304369d6591a" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRenewalTerm_019df4d8-0ed6-7811-8fcf-65fb2752d99b" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRenewalTerm_019df4d8-0ed6-7811-8fcf-65fb2752d99b" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfCountriesUseAestheticsAndDermatologicalFields_019df4d8-0ed6-73e9-a03c-840a55070de2" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfCountriesUseAestheticsAndDermatologicalFields"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfCountriesUseAestheticsAndDermatologicalFields_019df4d8-0ed6-73e9-a03c-840a55070de2" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_StockIssuedDuringPeriodSharesIssuedForLicenseSupplyAndDistributionAgreement_019df4d8-0ed6-7442-aff8-38e1d7868b8b" xlink:href="eols-20260630.xsd#eols_StockIssuedDuringPeriodSharesIssuedForLicenseSupplyAndDistributionAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_StockIssuedDuringPeriodSharesIssuedForLicenseSupplyAndDistributionAgreement_019df4d8-0ed6-7442-aff8-38e1d7868b8b" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment_019df4d8-0ed6-7e3f-9f18-ac189fe5deeb" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment_019df4d8-0ed6-7e3f-9f18-ac189fe5deeb" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue_019df4d8-0ed6-7f9a-a61f-ed05725963ab" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue_019df4d8-0ed6-7f9a-a61f-ed05725963ab" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_NonCashInResearchAndDevelopmentInProcessExpenses_019df4d8-0ed6-7669-ae4b-dfa8cbac7961" xlink:href="eols-20260630.xsd#eols_NonCashInResearchAndDevelopmentInProcessExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_NonCashInResearchAndDevelopmentInProcessExpenses_019df4d8-0ed6-7669-ae4b-dfa8cbac7961" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_019df4d8-0ed6-7050-9f32-c5463f8a7623" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinitelivedIntangibleAssetsAcquired1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_019df4d8-0ed6-7050-9f32-c5463f8a7623" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_019df4d8-0ed6-74cf-9551-100be3b3b519" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_019df4d8-0ed6-74cf-9551-100be3b3b519" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementPendingRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-703b-a114-27ff83f2847d" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementPendingRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementPendingRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-703b-a114-27ff83f2847d" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7086-a181-e5ae980c4c85" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7086-a181-e5ae980c4c85" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod_019df4d8-0ed6-737f-a0d4-f53c53db395a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod_019df4d8-0ed6-737f-a0d4-f53c53db395a" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent_019df4d8-0ed6-749d-97ea-e3decf9b97e1" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent_019df4d8-0ed6-749d-97ea-e3decf9b97e1" xlink:type="arc" order="34"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementIntangibleAsset_019df4d8-0ed6-7e74-9579-54fb03d89bf4" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementIntangibleAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementIntangibleAsset_019df4d8-0ed6-7e74-9579-54fb03d89bf4" xlink:type="arc" order="35"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill_019df4d8-0ed6-72ba-aab1-91ae66b773f5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill_019df4d8-0ed6-72ba-aab1-91ae66b773f5" xlink:type="arc" order="36"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_RelatedPartyTransactionPeriodOfTerminationOfFirstCommercialSale_019df4d8-0ed6-75e5-bc41-98eebdf7b898" xlink:href="eols-20260630.xsd#eols_RelatedPartyTransactionPeriodOfTerminationOfFirstCommercialSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_RelatedPartyTransactionPeriodOfTerminationOfFirstCommercialSale_019df4d8-0ed6-75e5-bc41-98eebdf7b898" xlink:type="arc" order="37"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_019df4d8-0ed6-79e3-ad34-0b28dd8f0b17" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_019df4d8-0ed6-79e3-ad34-0b28dd8f0b17" xlink:type="arc" order="38"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_RebatesAndCouponsCreditsAndPayments_019df4d8-0ed6-7c6f-a24a-60044a18f3e8" xlink:href="eols-20260630.xsd#eols_RebatesAndCouponsCreditsAndPayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_RebatesAndCouponsCreditsAndPayments_019df4d8-0ed6-7c6f-a24a-60044a18f3e8" xlink:type="arc" order="39"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PaymentsForProvisionsForAccruedVolumeBasedRebateAndCouponLiability_019df4d8-0ed6-704f-921a-513f61a4e323" xlink:href="eols-20260630.xsd#eols_PaymentsForProvisionsForAccruedVolumeBasedRebateAndCouponLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_PaymentsForProvisionsForAccruedVolumeBasedRebateAndCouponLiability_019df4d8-0ed6-704f-921a-513f61a4e323" xlink:type="arc" order="40"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_019df4d8-0ed6-76a2-9b7f-c5b054f10d27" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractWithCustomerLiabilityRevenueRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_019df4d8-0ed6-76a2-9b7f-c5b054f10d27" xlink:type="arc" order="41"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_019df4d8-0ed6-70be-8f18-da01d4e3ab55" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_019df4d8-0ed6-70be-8f18-da01d4e3ab55" xlink:type="arc" order="42"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_019df4d8-0ed6-7161-adc8-0ba2c6363813" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_019df4d8-0ed6-7161-adc8-0ba2c6363813" xlink:type="arc" order="43"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_AccountsReceivableAllowanceForCreditLossWriteOffRecoveryNet_019df4d8-0ed6-7730-9d09-8a212e6db74c" xlink:href="eols-20260630.xsd#eols_AccountsReceivableAllowanceForCreditLossWriteOffRecoveryNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_AccountsReceivableAllowanceForCreditLossWriteOffRecoveryNet_019df4d8-0ed6-7730-9d09-8a212e6db74c" xlink:type="arc" order="44"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_019df4d8-0ed6-770e-b6ca-84ce24c6e681" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_019df4d8-0ed6-770e-b6ca-84ce24c6e681" xlink:type="arc" order="45"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0ed6-7a33-bf99-8fc94e47e981" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0ed6-7a33-bf99-8fc94e47e981" xlink:type="arc" order="46"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019df4d8-0ed6-7466-a923-594cc153bc4b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019df4d8-0ed6-7466-a923-594cc153bc4b" xlink:type="arc" order="47"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesDetails_1" xlink:type="simple" xlink:href="eols-20260630.xsd#BasisofPresentationandSummaryofSignificantAccountingPoliciesDetails_1"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/BasisofPresentationandSummaryofSignificantAccountingPoliciesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0e1a-7ef7-9459-c504796646d3_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:to="loc_srt_RangeMember_019df4d8-0e1a-7ef7-9459-c504796646d3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019df4d8-0e1a-7ef7-9459-c504796646d3" xlink:to="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019df4d8-0e1a-74a1-b0ea-31f2052d099e" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:to="loc_srt_MinimumMember_019df4d8-0e1a-74a1-b0ea-31f2052d099e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019df4d8-0e1a-7078-a189-d0c04f219d5e" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0e1a-7f7e-a569-4f15f22d8173" xlink:to="loc_srt_MaximumMember_019df4d8-0e1a-7078-a189-d0c04f219d5e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-725d-8430-a708634c81dd_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-725d-8430-a708634c81dd_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0e1a-725d-8430-a708634c81dd" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_019df4d8-0e1a-7eb0-a236-1e421e79e8d8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:to="loc_us-gaap_ComputerEquipmentMember_019df4d8-0e1a-7eb0-a236-1e421e79e8d8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_019df4d8-0e1a-7b08-b934-8497ee642f39" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:to="loc_us-gaap_EquipmentMember_019df4d8-0e1a-7b08-b934-8497ee642f39" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_019df4d8-0e1a-73c3-b493-b97471b87d71" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0e1a-745a-b9cb-c6a3fa4bc531" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_019df4d8-0e1a-73c3-b493-b97471b87d71" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7882-acdd-cdfb2208125e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7882-acdd-cdfb2208125e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0e1a-7882-acdd-cdfb2208125e" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseU.S.AgreementMember_019df4d8-0e1a-7525-8cf7-30b19b531f4c" xlink:href="eols-20260630.xsd#eols_SymateseU.S.AgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:to="loc_eols_SymateseU.S.AgreementMember_019df4d8-0e1a-7525-8cf7-30b19b531f4c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseEuropeAgreementMember_019df4d8-0e1a-7778-8df8-20178f7afa2b" xlink:href="eols-20260630.xsd#eols_SymateseEuropeAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0e1a-7537-a4cb-1b8808c19809" xlink:to="loc_eols_SymateseEuropeAgreementMember_019df4d8-0e1a-7778-8df8-20178f7afa2b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:to="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-71cb-b907-0b7d2ecdd30b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_019df4d8-0e1a-7ae6-b7ee-fdf55ba0848b" xlink:to="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-71cb-b907-0b7d2ecdd30b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_019df4d8-0e1a-7fa1-93de-c2835d84c213" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_019df4d8-0e1a-71cb-b907-0b7d2ecdd30b" xlink:to="loc_us-gaap_CommonStockMember_019df4d8-0e1a-7fa1-93de-c2835d84c213" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-7cac-a488-ea9bb8468d8f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-7cac-a488-ea9bb8468d8f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0e1a-7cac-a488-ea9bb8468d8f" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DistributionRightsEvolysseNasolabialFoldProductMember_019df4d8-0e1a-787a-a50b-529d521076d9" xlink:href="eols-20260630.xsd#eols_DistributionRightsEvolysseNasolabialFoldProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_eols_DistributionRightsEvolysseNasolabialFoldProductMember_019df4d8-0e1a-787a-a50b-529d521076d9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DistributionRightsMember_019df4d8-0e1a-72b3-9f44-549ae7ecdd02" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DistributionRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_us-gaap_DistributionRightsMember_019df4d8-0e1a-72b3-9f44-549ae7ecdd02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DistributionRightsEvolysseProductsMember_019df4d8-0e1a-7b37-bfaa-70d0c60e66d3" xlink:href="eols-20260630.xsd#eols_DistributionRightsEvolysseProductsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_eols_DistributionRightsEvolysseProductsMember_019df4d8-0e1a-7b37-bfaa-70d0c60e66d3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7718-91f4-1f112a5feeab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SoftwareDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0e1a-734b-b7d6-087c9e32aa82" xlink:to="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7718-91f4-1f112a5feeab" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:href="eols-20260630.xsd#eols_MilestoneAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneDomain_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f_default" xlink:href="eols-20260630.xsd#eols_MilestoneDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:to="loc_eols_MilestoneDomain_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:href="eols-20260630.xsd#eols_MilestoneDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_eols_MilestoneAxis_019df4d8-0ed6-7a6d-afa5-9cde2f500e0f" xlink:to="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_FirstMilestonePaymentMember_019df4d8-0ed6-7044-9557-7b2dee11c852" xlink:href="eols-20260630.xsd#eols_FirstMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:to="loc_eols_FirstMilestonePaymentMember_019df4d8-0ed6-7044-9557-7b2dee11c852" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SecondMilestonePaymentMember_019df4d8-0ed6-7061-9d90-1370ee32fed2" xlink:href="eols-20260630.xsd#eols_SecondMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_MilestoneDomain_019df4d8-0ed6-7d72-a19f-fdf4085bf4bb" xlink:to="loc_eols_SecondMilestonePaymentMember_019df4d8-0ed6-7061-9d90-1370ee32fed2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-7a6e-b6ad-055a92a9f420_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-7a6e-b6ad-055a92a9f420_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-73a7-a2ea-9682f236e062" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionAcquireeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_BusinessAcquisitionAxis_019df4d8-0ed6-7a6e-b6ad-055a92a9f420" xlink:to="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-73a7-a2ea-9682f236e062" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_EvolusIncMember_019df4d8-0ed6-7375-a4ea-a1132884909b" xlink:href="eols-20260630.xsd#eols_EvolusIncMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionAcquireeDomain_019df4d8-0ed6-73a7-a2ea-9682f236e062" xlink:to="loc_eols_EvolusIncMember_019df4d8-0ed6-7375-a4ea-a1132884909b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff_default" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:to="loc_dei_EntityDomain_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019df4d8-0ed6-7a53-b5e5-a7dd95f755a6" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019df4d8-0ed6-7a30-91a8-6f9fa18e1dff" xlink:to="loc_dei_EntityDomain_019df4d8-0ed6-7a53-b5e5-a7dd95f755a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SCHAEONLLCMember_019df4d8-0ed6-7ad4-8f69-5bf04ff7683f" xlink:href="eols-20260630.xsd#eols_SCHAEONLLCMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019df4d8-0ed6-7a53-b5e5-a7dd95f755a6" xlink:to="loc_eols_SCHAEONLLCMember_019df4d8-0ed6-7ad4-8f69-5bf04ff7683f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-756f-ac19-96a08da7132c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-7ca4-86ad-b82bd3fc32a9" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-756f-ac19-96a08da7132c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PerformanceRestrictedStockUnitsPRSUMember_019df4d8-0ed6-7d6d-8c52-94bc7c71c2ee" xlink:href="eols-20260630.xsd#eols_PerformanceRestrictedStockUnitsPRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-756f-ac19-96a08da7132c" xlink:to="loc_eols_PerformanceRestrictedStockUnitsPRSUMember_019df4d8-0ed6-7d6d-8c52-94bc7c71c2ee" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-74d0-8b6b-81bee167939a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-74d0-8b6b-81bee167939a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_019df4d8-0ed6-74d0-8b6b-81bee167939a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_019df4d8-0ed6-7cf7-a554-b5a0b4171c87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:to="loc_us-gaap_EmployeeStockOptionMember_019df4d8-0ed6-7cf7-a554-b5a0b4171c87" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019df4d8-0ed6-7144-973e-9a1d265cceaa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_019df4d8-0ed6-778f-9567-1cd9426da4bd" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019df4d8-0ed6-7144-973e-9a1d265cceaa" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingenciesByNatureOfContingencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0e1a-774f-9940-175e57bc3cb4" xlink:to="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-7376-8b52-a82c52f1314c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:to="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-7376-8b52-a82c52f1314c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-75b0-ac35-b63e41fdfda0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyNatureDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LossContingenciesByNatureOfContingencyAxis_019fcee1-654e-7376-8b52-a82c52f1314c" xlink:to="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-75b0-ac35-b63e41fdfda0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_IEEPATariffRefundMember_019f8d03-3f79-7ccc-8949-528a61c975df" xlink:href="eols-20260630.xsd#eols_IEEPATariffRefundMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingencyNatureDomain_019fcee1-654e-75b0-ac35-b63e41fdfda0" xlink:to="loc_eols_IEEPATariffRefundMember_019f8d03-3f79-7ccc-8949-528a61c975df" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f6eb5-5f06-704f-a509-01dcff861a21" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f6eb5-5f06-704f-a509-01dcff861a21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_019df4d8-0ed6-7abb-8704-b92e2ee691c0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_NumberOfReportableSegments_019df4d8-0ed6-7abb-8704-b92e2ee691c0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_019df4d8-0ed6-7ace-83c8-7d40c403d49b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_NumberOfOperatingSegments_019df4d8-0ed6-7ace-83c8-7d40c403d49b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_AccountsReceivableCreditCardTransactionConvertedToCashPeriod_019df4d8-0ed6-7488-baa0-20d6e7c9701a" xlink:href="eols-20260630.xsd#eols_AccountsReceivableCreditCardTransactionConvertedToCashPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_AccountsReceivableCreditCardTransactionConvertedToCashPeriod_019df4d8-0ed6-7488-baa0-20d6e7c9701a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivableForRecoveryOfImportDutiesNet_019f6e9e-a7d0-7e2d-9b6b-aa4ff3a63f1d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ReceivableForRecoveryOfImportDutiesNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ReceivableForRecoveryOfImportDutiesNet_019f6e9e-a7d0-7e2d-9b6b-aa4ff3a63f1d" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RecoveryOfDirectCosts_019f6e9e-a7d0-7d52-a6fd-340c32bd3af2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RecoveryOfDirectCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_RecoveryOfDirectCosts_019f6e9e-a7d0-7d52-a6fd-340c32bd3af2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_IEEPATariffRefundOperatingOffset_019fcedb-e7f2-7719-95b3-63bae42c4a9a" xlink:href="eols-20260630.xsd#eols_IEEPATariffRefundOperatingOffset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_IEEPATariffRefundOperatingOffset_019fcedb-e7f2-7719-95b3-63bae42c4a9a" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryAdjustments_019f6e9e-a7d0-7b0c-b85a-1af3b968994d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InventoryAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_InventoryAdjustments_019f6e9e-a7d0-7b0c-b85a-1af3b968994d" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_019df4d8-0ed6-7da6-b234-53d9446ed20d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_019df4d8-0ed6-7da6-b234-53d9446ed20d" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportingUnits_019df4d8-0ed6-765e-8673-bf78684de972" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportingUnits"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_NumberOfReportingUnits_019df4d8-0ed6-765e-8673-bf78684de972" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillImpairmentLoss_019df4d8-0ed6-739b-b288-7c2ace21f8e0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GoodwillImpairmentLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_GoodwillImpairmentLoss_019df4d8-0ed6-739b-b288-7c2ace21f8e0" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment_019df4d8-0ed6-78cd-a95c-e62f70a17e6e" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment_019df4d8-0ed6-78cd-a95c-e62f70a17e6e" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement_019df4d8-0ed6-7e37-93cc-b1f3d627276b" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement_019df4d8-0ed6-7e37-93cc-b1f3d627276b" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod_019df4d8-0ed6-754c-ae88-96ffeb523a3a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod_019df4d8-0ed6-754c-ae88-96ffeb523a3a" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne_019df4d8-0ed6-74ac-9239-f4f1435fb783" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne_019df4d8-0ed6-74ac-9239-f4f1435fb783" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo_019df4d8-0ed6-7978-a924-f4ee5d80a442" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo_019df4d8-0ed6-7978-a924-f4ee5d80a442" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree_019df4d8-0ed6-7e4e-a966-860caa505852" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree_019df4d8-0ed6-7e4e-a966-860caa505852" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour_019df4d8-0ed6-7d8b-919f-7ee215d9c265" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour_019df4d8-0ed6-7d8b-919f-7ee215d9c265" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7702-8e4d-f64fe3a20173" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7702-8e4d-f64fe3a20173" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense_019df4d8-0ed6-7368-84ee-9bfff4696192" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense_019df4d8-0ed6-7368-84ee-9bfff4696192" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts_019df4d8-0ed6-7a0e-9ca9-376a4edcb08f" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts_019df4d8-0ed6-7a0e-9ca9-376a4edcb08f" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment_019df4d8-0ed6-76a1-bcf5-9dd82436fd96" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment_019df4d8-0ed6-76a1-bcf5-9dd82436fd96" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialTerm_019df4d8-0ed6-71e0-be60-304369d6591a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialTerm_019df4d8-0ed6-71e0-be60-304369d6591a" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRenewalTerm_019df4d8-0ed6-7811-8fcf-65fb2752d99b" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRenewalTerm_019df4d8-0ed6-7811-8fcf-65fb2752d99b" xlink:type="arc" order="23"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfCountriesUseAestheticsAndDermatologicalFields_019df4d8-0ed6-73e9-a03c-840a55070de2" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfCountriesUseAestheticsAndDermatologicalFields"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfCountriesUseAestheticsAndDermatologicalFields_019df4d8-0ed6-73e9-a03c-840a55070de2" xlink:type="arc" order="24"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_StockIssuedDuringPeriodSharesIssuedForLicenseSupplyAndDistributionAgreement_019df4d8-0ed6-7442-aff8-38e1d7868b8b" xlink:href="eols-20260630.xsd#eols_StockIssuedDuringPeriodSharesIssuedForLicenseSupplyAndDistributionAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_StockIssuedDuringPeriodSharesIssuedForLicenseSupplyAndDistributionAgreement_019df4d8-0ed6-7442-aff8-38e1d7868b8b" xlink:type="arc" order="25"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment_019df4d8-0ed6-7e3f-9f18-ac189fe5deeb" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment_019df4d8-0ed6-7e3f-9f18-ac189fe5deeb" xlink:type="arc" order="26"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue_019df4d8-0ed6-7f9a-a61f-ed05725963ab" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue_019df4d8-0ed6-7f9a-a61f-ed05725963ab" xlink:type="arc" order="27"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_NonCashInResearchAndDevelopmentInProcessExpenses_019df4d8-0ed6-7669-ae4b-dfa8cbac7961" xlink:href="eols-20260630.xsd#eols_NonCashInResearchAndDevelopmentInProcessExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_NonCashInResearchAndDevelopmentInProcessExpenses_019df4d8-0ed6-7669-ae4b-dfa8cbac7961" xlink:type="arc" order="28"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_019df4d8-0ed6-7050-9f32-c5463f8a7623" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FinitelivedIntangibleAssetsAcquired1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_FinitelivedIntangibleAssetsAcquired1_019df4d8-0ed6-7050-9f32-c5463f8a7623" xlink:type="arc" order="29"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_019df4d8-0ed6-74cf-9551-100be3b3b519" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_019df4d8-0ed6-74cf-9551-100be3b3b519" xlink:type="arc" order="30"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementPendingRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-703b-a114-27ff83f2847d" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementPendingRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementPendingRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-703b-a114-27ff83f2847d" xlink:type="arc" order="31"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7086-a181-e5ae980c4c85" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7086-a181-e5ae980c4c85" xlink:type="arc" order="32"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod_019df4d8-0ed6-737f-a0d4-f53c53db395a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod_019df4d8-0ed6-737f-a0d4-f53c53db395a" xlink:type="arc" order="33"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent_019df4d8-0ed6-749d-97ea-e3decf9b97e1" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent_019df4d8-0ed6-749d-97ea-e3decf9b97e1" xlink:type="arc" order="34"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementIntangibleAsset_019df4d8-0ed6-7e74-9579-54fb03d89bf4" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementIntangibleAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementIntangibleAsset_019df4d8-0ed6-7e74-9579-54fb03d89bf4" xlink:type="arc" order="35"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill_019df4d8-0ed6-72ba-aab1-91ae66b773f5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ImpairmentOfIntangibleAssetsExcludingGoodwill_019df4d8-0ed6-72ba-aab1-91ae66b773f5" xlink:type="arc" order="36"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_RelatedPartyTransactionPeriodOfTerminationOfFirstCommercialSale_019df4d8-0ed6-75e5-bc41-98eebdf7b898" xlink:href="eols-20260630.xsd#eols_RelatedPartyTransactionPeriodOfTerminationOfFirstCommercialSale"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_RelatedPartyTransactionPeriodOfTerminationOfFirstCommercialSale_019df4d8-0ed6-75e5-bc41-98eebdf7b898" xlink:type="arc" order="37"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_019df4d8-0ed6-79e3-ad34-0b28dd8f0b17" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_019df4d8-0ed6-79e3-ad34-0b28dd8f0b17" xlink:type="arc" order="38"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_RebatesAndCouponsCreditsAndPayments_019df4d8-0ed6-7c6f-a24a-60044a18f3e8" xlink:href="eols-20260630.xsd#eols_RebatesAndCouponsCreditsAndPayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_RebatesAndCouponsCreditsAndPayments_019df4d8-0ed6-7c6f-a24a-60044a18f3e8" xlink:type="arc" order="39"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PaymentsForProvisionsForAccruedVolumeBasedRebateAndCouponLiability_019df4d8-0ed6-704f-921a-513f61a4e323" xlink:href="eols-20260630.xsd#eols_PaymentsForProvisionsForAccruedVolumeBasedRebateAndCouponLiability"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_PaymentsForProvisionsForAccruedVolumeBasedRebateAndCouponLiability_019df4d8-0ed6-704f-921a-513f61a4e323" xlink:type="arc" order="40"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_019df4d8-0ed6-76a2-9b7f-c5b054f10d27" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractWithCustomerLiabilityRevenueRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityRevenueRecognized_019df4d8-0ed6-76a2-9b7f-c5b054f10d27" xlink:type="arc" order="41"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_019df4d8-0ed6-70be-8f18-da01d4e3ab55" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_019df4d8-0ed6-70be-8f18-da01d4e3ab55" xlink:type="arc" order="42"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProvisionForDoubtfulAccounts_019df4d8-0ed6-7161-adc8-0ba2c6363813" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProvisionForDoubtfulAccounts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ProvisionForDoubtfulAccounts_019df4d8-0ed6-7161-adc8-0ba2c6363813" xlink:type="arc" order="43"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_AccountsReceivableAllowanceForCreditLossWriteOffRecoveryNet_019df4d8-0ed6-7730-9d09-8a212e6db74c" xlink:href="eols-20260630.xsd#eols_AccountsReceivableAllowanceForCreditLossWriteOffRecoveryNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_eols_AccountsReceivableAllowanceForCreditLossWriteOffRecoveryNet_019df4d8-0ed6-7730-9d09-8a212e6db74c" xlink:type="arc" order="44"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_019df4d8-0ed6-770e-b6ca-84ce24c6e681" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_019df4d8-0ed6-770e-b6ca-84ce24c6e681" xlink:type="arc" order="45"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0ed6-7a33-bf99-8fc94e47e981" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0ed6-7a33-bf99-8fc94e47e981" xlink:type="arc" order="46"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019df4d8-0ed6-7466-a923-594cc153bc4b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-7340-8135-50dfa1a20c1a" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_019df4d8-0ed6-7466-a923-594cc153bc4b" xlink:type="arc" order="47"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/FairValueMeasurementsScheduleofFairValueofInstrumentsDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#FairValueMeasurementsScheduleofFairValueofInstrumentsDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/FairValueMeasurementsScheduleofFairValueofInstrumentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-708e-b5d2-b2c44946aa6a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-7614-bec2-408a440370b0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-708e-b5d2-b2c44946aa6a" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-7614-bec2-408a440370b0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019df4d8-0ed6-7dfe-a8e6-e1465a4d9137" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-7614-bec2-408a440370b0" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019df4d8-0ed6-7dfe-a8e6-e1465a4d9137" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-7dfe-a8e6-e1465a4d9137_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019df4d8-0ed6-7dfe-a8e6-e1465a4d9137" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-7dfe-a8e6-e1465a4d9137_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-76a1-9bc0-60f157e96558" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019df4d8-0ed6-7dfe-a8e6-e1465a4d9137" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-76a1-9bc0-60f157e96558" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019df4d8-0ed6-71ff-9550-e6303a78e467" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-76a1-9bc0-60f157e96558" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019df4d8-0ed6-71ff-9550-e6303a78e467" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019df4d8-0ed6-7e61-b578-adaf072a7feb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-76a1-9bc0-60f157e96558" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019df4d8-0ed6-7e61-b578-adaf072a7feb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019df4d8-0ed6-78e8-9e8d-a9bcd167c100" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019df4d8-0ed6-76a1-9bc0-60f157e96558" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019df4d8-0ed6-78e8-9e8d-a9bcd167c100" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ContingentRoyaltyObligationPayableToRelatedPartyFairValue_019df4d8-0ed6-7bd6-9e15-0636e980236a" xlink:href="eols-20260630.xsd#eols_ContingentRoyaltyObligationPayableToRelatedPartyFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-708e-b5d2-b2c44946aa6a" xlink:to="loc_eols_ContingentRoyaltyObligationPayableToRelatedPartyFairValue_019df4d8-0ed6-7bd6-9e15-0636e980236a" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#FairValueMeasurementsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/FairValueMeasurementsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-7bbe-871f-191f54b51658" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-770b-ba65-499fc5dd291c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-7bbe-871f-191f54b51658" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-770b-ba65-499fc5dd291c" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeAxis_019df4d8-0ed6-72fc-987c-2922f264879a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-770b-ba65-499fc5dd291c" xlink:to="loc_us-gaap_MeasurementInputTypeAxis_019df4d8-0ed6-72fc-987c-2922f264879a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019df4d8-0ed6-72fc-987c-2922f264879a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019df4d8-0ed6-72fc-987c-2922f264879a" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019df4d8-0ed6-72fc-987c-2922f264879a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputTypeDomain_019df4d8-0ed6-7ba1-a393-d51bba26d8f3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_MeasurementInputTypeAxis_019df4d8-0ed6-72fc-987c-2922f264879a" xlink:to="loc_us-gaap_MeasurementInputTypeDomain_019df4d8-0ed6-7ba1-a393-d51bba26d8f3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MeasurementInputDiscountRateMember_019df4d8-0ed6-724f-8be6-8e67f537e534" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MeasurementInputDiscountRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_MeasurementInputTypeDomain_019df4d8-0ed6-7ba1-a393-d51bba26d8f3" xlink:to="loc_us-gaap_MeasurementInputDiscountRateMember_019df4d8-0ed6-724f-8be6-8e67f537e534" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019df4d8-0ed6-7f56-9ef6-c82e9007a2a2" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-770b-ba65-499fc5dd291c" xlink:to="loc_srt_RangeAxis_019df4d8-0ed6-7f56-9ef6-c82e9007a2a2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0ed6-7f56-9ef6-c82e9007a2a2_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019df4d8-0ed6-7f56-9ef6-c82e9007a2a2" xlink:to="loc_srt_RangeMember_019df4d8-0ed6-7f56-9ef6-c82e9007a2a2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0ed6-72eb-a4bb-7efc4ba3e977" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019df4d8-0ed6-7f56-9ef6-c82e9007a2a2" xlink:to="loc_srt_RangeMember_019df4d8-0ed6-72eb-a4bb-7efc4ba3e977" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019df4d8-0ed6-7709-b251-f893b4d4255a" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0ed6-72eb-a4bb-7efc4ba3e977" xlink:to="loc_srt_MaximumMember_019df4d8-0ed6-7709-b251-f893b4d4255a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementBasisAxis_019df4d8-0ed6-7cc0-880c-f038b99631ac" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueByMeasurementBasisAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-770b-ba65-499fc5dd291c" xlink:to="loc_us-gaap_FairValueByMeasurementBasisAxis_019df4d8-0ed6-7cc0-880c-f038b99631ac" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019df4d8-0ed6-7cc0-880c-f038b99631ac_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_019df4d8-0ed6-7cc0-880c-f038b99631ac" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019df4d8-0ed6-7cc0-880c-f038b99631ac_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019df4d8-0ed6-78df-b885-1826d4c0d8b6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueDisclosureItemAmountsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementBasisAxis_019df4d8-0ed6-7cc0-880c-f038b99631ac" xlink:to="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019df4d8-0ed6-78df-b885-1826d4c0d8b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019df4d8-0ed6-7052-91ea-678deee09684" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PortionAtFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019df4d8-0ed6-78df-b885-1826d4c0d8b6" xlink:to="loc_us-gaap_PortionAtFairValueFairValueDisclosureMember_019df4d8-0ed6-7052-91ea-678deee09684" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_019df4d8-0ed6-7dbd-8ee2-c193e74b5aba" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EstimateOfFairValueFairValueDisclosureMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueDisclosureItemAmountsDomain_019df4d8-0ed6-78df-b885-1826d4c0d8b6" xlink:to="loc_us-gaap_EstimateOfFairValueFairValueDisclosureMember_019df4d8-0ed6-7dbd-8ee2-c193e74b5aba" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7ec9-85e2-2b9afce9c80f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_019df4d8-0ed6-770b-ba65-499fc5dd291c" xlink:to="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7ec9-85e2-2b9afce9c80f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7ec9-85e2-2b9afce9c80f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7ec9-85e2-2b9afce9c80f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7ec9-85e2-2b9afce9c80f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7b23-81bd-906b38c2df07" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7ec9-85e2-2b9afce9c80f" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7b23-81bd-906b38c2df07" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PharmakonTermLoansMember_019df4d8-0ed6-7534-8e5e-d0eb3b558d31" xlink:href="eols-20260630.xsd#eols_PharmakonTermLoansMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7b23-81bd-906b38c2df07" xlink:to="loc_eols_PharmakonTermLoansMember_019df4d8-0ed6-7534-8e5e-d0eb3b558d31" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ContingentLiabilityToRelatedPartyMeasurementInput_019df4d8-0ed6-77f8-b0f5-08bb19a8c884" xlink:href="eols-20260630.xsd#eols_ContingentLiabilityToRelatedPartyMeasurementInput"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-7bbe-871f-191f54b51658" xlink:to="loc_eols_ContingentLiabilityToRelatedPartyMeasurementInput_019df4d8-0ed6-77f8-b0f5-08bb19a8c884" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationsRecurringBasisLiabilityValue_019df4d8-0ed6-774f-b050-2660debfd78c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationsRecurringBasisLiabilityValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_019df4d8-0ed6-7bbe-871f-191f54b51658" xlink:to="loc_us-gaap_FairValueMeasurementWithUnobservableInputsReconciliationsRecurringBasisLiabilityValue_019df4d8-0ed6-774f-b050-2660debfd78c" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/GoodwillandIntangibleAssetsScheduleofDefiniteandIndefiniteLivedIntangibleAssetsDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#GoodwillandIntangibleAssetsScheduleofDefiniteandIndefiniteLivedIntangibleAssetsDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/GoodwillandIntangibleAssetsScheduleofDefiniteandIndefiniteLivedIntangibleAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_019df4d8-0ed6-7e0e-865d-6fd8f0f9ff5a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_019df4d8-0ed6-7832-a12d-29fd34f7df67" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_019df4d8-0ed6-7e0e-865d-6fd8f0f9ff5a" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_019df4d8-0ed6-7832-a12d-29fd34f7df67" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7ce9-81bd-fc6bf0fba351" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_019df4d8-0ed6-7832-a12d-29fd34f7df67" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7ce9-81bd-fc6bf0fba351" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7ce9-81bd-fc6bf0fba351_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7ce9-81bd-fc6bf0fba351" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7ce9-81bd-fc6bf0fba351_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7801-94b7-951aae83edd2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7ce9-81bd-fc6bf0fba351" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7801-94b7-951aae83edd2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DistributionRightsMember_019df4d8-0ed6-7d55-a6c7-cac8e33ffbb2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DistributionRightsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7801-94b7-951aae83edd2" xlink:to="loc_us-gaap_DistributionRightsMember_019df4d8-0ed6-7d55-a6c7-cac8e33ffbb2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7bb3-a9f0-cad7a0869d7f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SoftwareDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7801-94b7-951aae83edd2" xlink:to="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7bb3-a9f0-cad7a0869d7f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNetAbstract_019df4d8-0ed6-7d48-8ea9-985fad120b1b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsNetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_019df4d8-0ed6-7e0e-865d-6fd8f0f9ff5a" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNetAbstract_019df4d8-0ed6-7d48-8ea9-985fad120b1b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_019df4d8-0ed6-7701-a5ed-054efe479084" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNetAbstract_019df4d8-0ed6-7d48-8ea9-985fad120b1b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_019df4d8-0ed6-7701-a5ed-054efe479084" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_019df4d8-0ed6-70de-b437-acc0065589e2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNetAbstract_019df4d8-0ed6-7d48-8ea9-985fad120b1b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_019df4d8-0ed6-70de-b437-acc0065589e2" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_019df4d8-0ed6-75f7-9f0f-157534402bc4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNetAbstract_019df4d8-0ed6-7d48-8ea9-985fad120b1b" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_019df4d8-0ed6-75f7-9f0f-157534402bc4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwillAbstract_019df4d8-0ed6-7781-954a-116830fde1e6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwillAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_019df4d8-0ed6-7e0e-865d-6fd8f0f9ff5a" xlink:to="loc_us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwillAbstract_019df4d8-0ed6-7781-954a-116830fde1e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_019df4d8-0ed6-73eb-ae6f-9866f89f575e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IndefiniteLivedIntangibleAssetsExcludingGoodwillAbstract_019df4d8-0ed6-7781-954a-116830fde1e6" xlink:to="loc_us-gaap_Goodwill_019df4d8-0ed6-73eb-ae6f-9866f89f575e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_IntangibleAssetsGrossIncludingGoodwill_019df4d8-0ed6-701b-82b8-7a9c328f51ef" xlink:href="eols-20260630.xsd#eols_IntangibleAssetsGrossIncludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_019df4d8-0ed6-7e0e-865d-6fd8f0f9ff5a" xlink:to="loc_eols_IntangibleAssetsGrossIncludingGoodwill_019df4d8-0ed6-701b-82b8-7a9c328f51ef" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetIncludingGoodwill_019df4d8-0ed6-7947-9c7d-17adc942077f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IntangibleAssetsNetIncludingGoodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_019df4d8-0ed6-7e0e-865d-6fd8f0f9ff5a" xlink:to="loc_us-gaap_IntangibleAssetsNetIncludingGoodwill_019df4d8-0ed6-7947-9c7d-17adc942077f" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/GoodwillandIntangibleAssetsNarrativeDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#GoodwillandIntangibleAssetsNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/GoodwillandIntangibleAssetsNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessAcquisitionLineItems_019df4d8-0ed6-7e34-91f1-8d6d38e82e94" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_BusinessAcquisitionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019df4d8-0ed6-7e19-b594-e77c4df6f498" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019df4d8-0ed6-7e34-91f1-8d6d38e82e94" xlink:to="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019df4d8-0ed6-7e19-b594-e77c4df6f498" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7e10-afe4-562df7238173" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfBusinessAcquisitionsByAcquisitionTable_019df4d8-0ed6-7e19-b594-e77c4df6f498" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7e10-afe4-562df7238173" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7e10-afe4-562df7238173_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7e10-afe4-562df7238173" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7e10-afe4-562df7238173_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7b4d-83b8-5f93a0b38757" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_019df4d8-0ed6-7e10-afe4-562df7238173" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7b4d-83b8-5f93a0b38757" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7934-89e4-00d345ae44b7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SoftwareDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_019df4d8-0ed6-7b4d-83b8-5f93a0b38757" xlink:to="loc_us-gaap_SoftwareDevelopmentMember_019df4d8-0ed6-7934-89e4-00d345ae44b7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_CapitalizedCostsIntangibleAssetsAddition_019df4d8-0ed6-7c71-bd91-6a4d614e0b16" xlink:href="eols-20260630.xsd#eols_CapitalizedCostsIntangibleAssetsAddition"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019df4d8-0ed6-7e34-91f1-8d6d38e82e94" xlink:to="loc_eols_CapitalizedCostsIntangibleAssetsAddition_019df4d8-0ed6-7c71-bd91-6a4d614e0b16" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_019df4d8-0ed6-713f-a03d-8adc19e3f368" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019df4d8-0ed6-7e34-91f1-8d6d38e82e94" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_019df4d8-0ed6-713f-a03d-8adc19e3f368" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfIntangibleAssets_019df4d8-0ed6-7e62-9a45-218b733ab698" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfIntangibleAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_BusinessAcquisitionLineItems_019df4d8-0ed6-7e34-91f1-8d6d38e82e94" xlink:to="loc_us-gaap_AmortizationOfIntangibleAssets_019df4d8-0ed6-7e62-9a45-218b733ab698" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/PropertyandEquipmentScheduleofPropertyandEquipmentDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#PropertyandEquipmentScheduleofPropertyandEquipmentDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/PropertyandEquipmentScheduleofPropertyandEquipmentDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-78cc-a1c0-b8819ebf9dd2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0ed6-7d61-a5ac-5b92368f8ba1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-78cc-a1c0-b8819ebf9dd2" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0ed6-7d61-a5ac-5b92368f8ba1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0ed6-7d5f-86ba-24ee42589e29" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_019df4d8-0ed6-7d61-a5ac-5b92368f8ba1" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0ed6-7d5f-86ba-24ee42589e29" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-7d5f-86ba-24ee42589e29_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0ed6-7d5f-86ba-24ee42589e29" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-7d5f-86ba-24ee42589e29_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_019df4d8-0ed6-7d5f-86ba-24ee42589e29" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquipmentMember_019df4d8-0ed6-76ea-b6dc-ac3fe3ef2e7e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:to="loc_us-gaap_EquipmentMember_019df4d8-0ed6-76ea-b6dc-ac3fe3ef2e7e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FurnitureAndFixturesMember_019df4d8-0ed6-7d85-a2d6-7440653ee103" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FurnitureAndFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:to="loc_us-gaap_FurnitureAndFixturesMember_019df4d8-0ed6-7d85-a2d6-7440653ee103" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_019df4d8-0ed6-7131-808a-0a5f33dd88bf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_019df4d8-0ed6-7131-808a-0a5f33dd88bf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_019df4d8-0ed6-7593-97a6-c403d592a388" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:to="loc_us-gaap_ComputerEquipmentMember_019df4d8-0ed6-7593-97a6-c403d592a388" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MarketingFixturesMember_019df4d8-0ed6-78b9-bb12-8124f013ed1e" xlink:href="eols-20260630.xsd#eols_MarketingFixturesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_019df4d8-0ed6-76a6-879a-890ee2994be0" xlink:to="loc_eols_MarketingFixturesMember_019df4d8-0ed6-78b9-bb12-8124f013ed1e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_019df4d8-0ed6-7fda-bf37-d18a33349dcc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-78cc-a1c0-b8819ebf9dd2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_019df4d8-0ed6-7fda-bf37-d18a33349dcc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_019df4d8-0ed6-7193-af27-6d0ce1958539" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-78cc-a1c0-b8819ebf9dd2" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_019df4d8-0ed6-7193-af27-6d0ce1958539" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_019df4d8-0ed6-7865-bc8a-198deb8e69dd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_019df4d8-0ed6-78cc-a1c0-b8819ebf9dd2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_019df4d8-0ed6-7865-bc8a-198deb8e69dd" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/LongTermDebtNarrativeDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#LongTermDebtNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/LongTermDebtNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7241-9336-9682ee36d1b2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7241-9336-9682ee36d1b2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7ac8-a777-1918d901f7a9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7241-9336-9682ee36d1b2" xlink:to="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7ac8-a777-1918d901f7a9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-7ac8-a777-1918d901f7a9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7ac8-a777-1918d901f7a9" xlink:to="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-7ac8-a777-1918d901f7a9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71c4-9a8e-ee61652f365a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7ac8-a777-1918d901f7a9" xlink:to="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71c4-9a8e-ee61652f365a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_019df4d8-0ed6-75f8-8799-8d8a6699b711" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71c4-9a8e-ee61652f365a" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_019df4d8-0ed6-75f8-8799-8d8a6699b711" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_019df4d8-0ed6-7d3b-99a2-8f3f441ce331" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71c4-9a8e-ee61652f365a" xlink:to="loc_us-gaap_SecuredDebtMember_019df4d8-0ed6-7d3b-99a2-8f3f441ce331" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7f94-8faf-5f0f7397bd36" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7241-9336-9682ee36d1b2" xlink:to="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7f94-8faf-5f0f7397bd36" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7f94-8faf-5f0f7397bd36_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7f94-8faf-5f0f7397bd36" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7f94-8faf-5f0f7397bd36_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-7f94-8faf-5f0f7397bd36" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LoanAgreementMember_019df4d8-0ed6-7e54-ac0e-eabe07bd289f" xlink:href="eols-20260630.xsd#eols_LoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:to="loc_eols_LoanAgreementMember_019df4d8-0ed6-7e54-ac0e-eabe07bd289f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-7e0d-824d-906e347ec6b4" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:to="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-7e0d-824d-906e347ec6b4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheOneMember_019df4d8-0ed6-7618-89f9-78300769b5e1" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheOneMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-7e0d-824d-906e347ec6b4" xlink:to="loc_eols_ARLoanAgreementTrancheOneMember_019df4d8-0ed6-7618-89f9-78300769b5e1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheTwoMember_019df4d8-0ed6-758f-a35b-4212753f18dc" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheTwoMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-7e0d-824d-906e347ec6b4" xlink:to="loc_eols_ARLoanAgreementTrancheTwoMember_019df4d8-0ed6-758f-a35b-4212753f18dc" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheThreeMember_019df4d8-0ed6-77b0-a6ac-4a2f4ddd2c0b" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-7e0d-824d-906e347ec6b4" xlink:to="loc_eols_ARLoanAgreementTrancheThreeMember_019df4d8-0ed6-77b0-a6ac-4a2f4ddd2c0b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementAmountsBorrowedUnderPriorTermLoanMember_019df4d8-0ed6-793e-829a-a37e6de01bac" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementAmountsBorrowedUnderPriorTermLoanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:to="loc_eols_ARLoanAgreementAmountsBorrowedUnderPriorTermLoanMember_019df4d8-0ed6-793e-829a-a37e6de01bac" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementIncrementalBorrowingsMember_019df4d8-0ed6-7941-87a2-e4db348b19b8" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementIncrementalBorrowingsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:to="loc_eols_ARLoanAgreementIncrementalBorrowingsMember_019df4d8-0ed6-7941-87a2-e4db348b19b8" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementTrancheTwoAndThreeMember_019df4d8-0ed6-7620-b8f4-745c3aba778f" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementTrancheTwoAndThreeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:to="loc_eols_ARLoanAgreementTrancheTwoAndThreeMember_019df4d8-0ed6-7620-b8f4-745c3aba778f" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PriorPharmakonLoanAgreementMember_019df4d8-0ed6-7d2f-9879-221d54df7e36" xlink:href="eols-20260630.xsd#eols_PriorPharmakonLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-79bb-a3d5-75c7deaf1c38" xlink:to="loc_eols_PriorPharmakonLoanAgreementMember_019df4d8-0ed6-7d2f-9879-221d54df7e36" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-76f3-865c-2b04fb803056" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7241-9336-9682ee36d1b2" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-76f3-865c-2b04fb803056" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-76f3-865c-2b04fb803056_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-76f3-865c-2b04fb803056" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-76f3-865c-2b04fb803056_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-73a9-a0bf-06bffa84b13f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-76f3-865c-2b04fb803056" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-73a9-a0bf-06bffa84b13f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_019df4d8-0ed6-76a5-b029-5496206f13b9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-73a9-a0bf-06bffa84b13f" xlink:to="loc_us-gaap_LineOfCreditMember_019df4d8-0ed6-76a5-b029-5496206f13b9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_019df4d8-0ed6-760b-a2a6-89b998d3b3be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_019df4d8-0ed6-760b-a2a6-89b998d3b3be" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityAccordionFeatureMaximumLimit_019df4d8-0ed6-76fd-9f75-f5fd9da100e5" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityAccordionFeatureMaximumLimit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_LineOfCreditFacilityAccordionFeatureMaximumLimit_019df4d8-0ed6-76fd-9f75-f5fd9da100e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityAccordionFeatureElectableIncrementAmount_019df4d8-0ed6-7e99-a8c8-c8ef0c83f403" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityAccordionFeatureElectableIncrementAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_LineOfCreditFacilityAccordionFeatureElectableIncrementAmount_019df4d8-0ed6-7e99-a8c8-c8ef0c83f403" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityMinimumUtilizationThresholdAmount_019df4d8-0ed6-7eee-9bd7-a58eb2edf41c" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityMinimumUtilizationThresholdAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_LineOfCreditFacilityMinimumUtilizationThresholdAmount_019df4d8-0ed6-7eee-9bd7-a58eb2edf41c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentBasisSpreadOnVariableRateFloor_019df4d8-0ed6-78be-9ea5-3e89f2cda1d2" xlink:href="eols-20260630.xsd#eols_DebtInstrumentBasisSpreadOnVariableRateFloor"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentBasisSpreadOnVariableRateFloor_019df4d8-0ed6-78be-9ea5-3e89f2cda1d2" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_019df4d8-0ed6-785b-a2c9-63defb6e7f1a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_019df4d8-0ed6-785b-a2c9-63defb6e7f1a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityClosingFeePercentage_019df4d8-0ed6-7100-aa09-f9b9b964f874" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityClosingFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_LineOfCreditFacilityClosingFeePercentage_019df4d8-0ed6-7100-aa09-f9b9b964f874" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityUnusedCapacityCommitmentFeePercentage_019df4d8-0ed6-77dd-85f7-7c18ad212f3e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditFacilityUnusedCapacityCommitmentFeePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_LineOfCreditFacilityUnusedCapacityCommitmentFeePercentage_019df4d8-0ed6-77dd-85f7-7c18ad212f3e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentPrepaymentFeePercentageYearOne_019df4d8-0ed6-7f79-9b99-299889388358" xlink:href="eols-20260630.xsd#eols_DebtInstrumentPrepaymentFeePercentageYearOne"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentPrepaymentFeePercentageYearOne_019df4d8-0ed6-7f79-9b99-299889388358" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentPrepaymentFeePercentageYearTwo_019df4d8-0ed6-7578-a6ed-57b79f517dc8" xlink:href="eols-20260630.xsd#eols_DebtInstrumentPrepaymentFeePercentageYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentPrepaymentFeePercentageYearTwo_019df4d8-0ed6-7578-a6ed-57b79f517dc8" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentPrepaymentFeePercentageAfterTwo_019df4d8-0ed6-7684-96d1-53204f778549" xlink:href="eols-20260630.xsd#eols_DebtInstrumentPrepaymentFeePercentageAfterTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentPrepaymentFeePercentageAfterTwo_019df4d8-0ed6-7684-96d1-53204f778549" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentLenderFeesAndDebtIssuanceCostsNet_019df4d8-0ed6-7c48-9f86-5bbfd7f260bd" xlink:href="eols-20260630.xsd#eols_DebtInstrumentLenderFeesAndDebtIssuanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentLenderFeesAndDebtIssuanceCostsNet_019df4d8-0ed6-7c48-9f86-5bbfd7f260bd" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentNumberOfTranches_019df4d8-0ed6-72f1-b07e-c7471d3d2aa0" xlink:href="eols-20260630.xsd#eols_DebtInstrumentNumberOfTranches"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentNumberOfTranches_019df4d8-0ed6-72f1-b07e-c7471d3d2aa0" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSecuredLinesOfCredit_019df4d8-0ed6-7ed7-805c-590e48e13da0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromSecuredLinesOfCredit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_ProceedsFromSecuredLinesOfCredit_019df4d8-0ed6-7ed7-805c-590e48e13da0" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ProceedsFromSecuredLinesOfCreditNetOfDiscountsAndFees_019df4d8-0ed6-7d97-a2d5-2b4569cd8747" xlink:href="eols-20260630.xsd#eols_ProceedsFromSecuredLinesOfCreditNetOfDiscountsAndFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_ProceedsFromSecuredLinesOfCreditNetOfDiscountsAndFees_019df4d8-0ed6-7d97-a2d5-2b4569cd8747" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LineOfCreditFacilityMinimumBorrowingCapacity_019df4d8-0ed6-7585-ab15-eef623645fb0" xlink:href="eols-20260630.xsd#eols_LineOfCreditFacilityMinimumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_LineOfCreditFacilityMinimumBorrowingCapacity_019df4d8-0ed6-7585-ab15-eef623645fb0" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentAdditionalConsiderationOnPrincipalDrawnPercentage_019df4d8-0ed6-7687-9d5b-641859816481" xlink:href="eols-20260630.xsd#eols_DebtInstrumentAdditionalConsiderationOnPrincipalDrawnPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentAdditionalConsiderationOnPrincipalDrawnPercentage_019df4d8-0ed6-7687-9d5b-641859816481" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentExitConsiderationMultiplier_019df4d8-0ed6-70c1-aa66-e1b93d7e2b62" xlink:href="eols-20260630.xsd#eols_DebtInstrumentExitConsiderationMultiplier"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentExitConsiderationMultiplier_019df4d8-0ed6-70c1-aa66-e1b93d7e2b62" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentDirectLenderFeesAndModificationCosts_019df4d8-0ed6-74c0-824b-c2d59755e099" xlink:href="eols-20260630.xsd#eols_DebtInstrumentDirectLenderFeesAndModificationCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentDirectLenderFeesAndModificationCosts_019df4d8-0ed6-74c0-824b-c2d59755e099" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentDirectLenderFees_019df4d8-0ed6-7403-a760-3a94d2c36c3d" xlink:href="eols-20260630.xsd#eols_DebtInstrumentDirectLenderFees"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentDirectLenderFees_019df4d8-0ed6-7403-a760-3a94d2c36c3d" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentModificationCosts_019df4d8-0ed6-7bb4-9012-856950fd9262" xlink:href="eols-20260630.xsd#eols_DebtInstrumentModificationCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_eols_DebtInstrumentModificationCosts_019df4d8-0ed6-7bb4-9012-856950fd9262" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_019df4d8-0ed6-7637-b63e-ef8ecaa9f92f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_DebtInstrumentInterestRateEffectivePercentage_019df4d8-0ed6-7637-b63e-ef8ecaa9f92f" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019df4d8-0ed6-75cb-9560-e2eb7653fa95" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_019df4d8-0ed6-75cb-9560-e2eb7653fa95" xlink:type="arc" order="22"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_019df4d8-0ed6-76ae-a3e0-935f132a21e7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-7bc5-8471-371fbbf6e887" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_019df4d8-0ed6-76ae-a3e0-935f132a21e7" xlink:type="arc" order="23"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/LongTermDebtScheduleofLongTermDebtObligationsDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#LongTermDebtScheduleofLongTermDebtObligationsDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/LongTermDebtScheduleofLongTermDebtObligationsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-754f-911f-801443e8d6de" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7500-9b9a-c6f0a9cd9c8f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-754f-911f-801443e8d6de" xlink:to="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7500-9b9a-c6f0a9cd9c8f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-73d5-bb1e-3011b99c4667" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7500-9b9a-c6f0a9cd9c8f" xlink:to="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-73d5-bb1e-3011b99c4667" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-73d5-bb1e-3011b99c4667_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-73d5-bb1e-3011b99c4667" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-73d5-bb1e-3011b99c4667_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7647-a660-6df3acdb102d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_DebtInstrumentAxis_019df4d8-0ed6-73d5-bb1e-3011b99c4667" xlink:to="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7647-a660-6df3acdb102d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-720c-b822-d5dc68634b05" xlink:href="eols-20260630.xsd#eols_ARLoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7647-a660-6df3acdb102d" xlink:to="loc_eols_ARLoanAgreementMember_019df4d8-0ed6-720c-b822-d5dc68634b05" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LoanAgreementMember_019df4d8-0ed6-7675-ac23-171cb7772469" xlink:href="eols-20260630.xsd#eols_LoanAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentNameDomain_019df4d8-0ed6-7647-a660-6df3acdb102d" xlink:to="loc_eols_LoanAgreementMember_019df4d8-0ed6-7675-ac23-171cb7772469" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-77bc-aa9c-d1c3e3cb2bd3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7500-9b9a-c6f0a9cd9c8f" xlink:to="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-77bc-aa9c-d1c3e3cb2bd3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-77bc-aa9c-d1c3e3cb2bd3_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-77bc-aa9c-d1c3e3cb2bd3" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-77bc-aa9c-d1c3e3cb2bd3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-7dfb-a49b-ab3330ebe965" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LongtermDebtTypeAxis_019df4d8-0ed6-77bc-aa9c-d1c3e3cb2bd3" xlink:to="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-7dfb-a49b-ab3330ebe965" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditMember_019df4d8-0ed6-73a2-a7b9-9469e17b8dcd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LineOfCreditMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LongtermDebtTypeDomain_019df4d8-0ed6-7dfb-a49b-ab3330ebe965" xlink:to="loc_us-gaap_LineOfCreditMember_019df4d8-0ed6-73a2-a7b9-9469e17b8dcd" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7028-ab1a-4305475fdd51" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtInstrumentTable_019df4d8-0ed6-7500-9b9a-c6f0a9cd9c8f" xlink:to="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7028-ab1a-4305475fdd51" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-7028-ab1a-4305475fdd51_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7028-ab1a-4305475fdd51" xlink:to="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-7028-ab1a-4305475fdd51_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71b5-86c0-4b1106dc54af" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_019df4d8-0ed6-7028-ab1a-4305475fdd51" xlink:to="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71b5-86c0-4b1106dc54af" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredDebtMember_019df4d8-0ed6-7743-9b77-b3aafc65d75c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SecuredDebtMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71b5-86c0-4b1106dc54af" xlink:to="loc_us-gaap_SecuredDebtMember_019df4d8-0ed6-7743-9b77-b3aafc65d75c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_019df4d8-0ed6-7979-955e-016c297ff8e3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_019df4d8-0ed6-71b5-86c0-4b1106dc54af" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_019df4d8-0ed6-7979-955e-016c297ff8e3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentCarryingAmount_019df4d8-0ed6-7602-a3a0-51fcd935f3e5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentCarryingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-754f-911f-801443e8d6de" xlink:to="loc_us-gaap_DebtInstrumentCarryingAmount_019df4d8-0ed6-7602-a3a0-51fcd935f3e5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredFinanceCostsNet_019df4d8-0ed6-7a81-8b94-56f7fd8444a3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DeferredFinanceCostsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-754f-911f-801443e8d6de" xlink:to="loc_us-gaap_DeferredFinanceCostsNet_019df4d8-0ed6-7a81-8b94-56f7fd8444a3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_DebtInstrumentExitConsideration_019df4d8-0ed6-7986-8520-5b2d79e8b6fb" xlink:href="eols-20260630.xsd#eols_DebtInstrumentExitConsideration"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-754f-911f-801443e8d6de" xlink:to="loc_eols_DebtInstrumentExitConsideration_019df4d8-0ed6-7986-8520-5b2d79e8b6fb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_019df4d8-0ed6-772b-8996-17bff5df8d67" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebt"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DebtInstrumentLineItems_019df4d8-0ed6-754f-911f-801443e8d6de" xlink:to="loc_us-gaap_LongTermDebt_019df4d8-0ed6-772b-8996-17bff5df8d67" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/CommitmentandContingenciesDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#CommitmentandContingenciesDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/CommitmentandContingenciesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingenciesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingenciesTable_019df4d8-0ed6-7a26-9aa0-0d975262b1bc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingenciesTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_us-gaap_LossContingenciesTable_019df4d8-0ed6-7a26-9aa0-0d975262b1bc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0ed6-7834-999b-d65f5f1cb05f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_019df4d8-0ed6-7a26-9aa0-0d975262b1bc" xlink:to="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0ed6-7834-999b-d65f5f1cb05f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0ed6-7834-999b-d65f5f1cb05f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0ed6-7834-999b-d65f5f1cb05f" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0ed6-7834-999b-d65f5f1cb05f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0ed6-74d9-9d05-87abf9f690b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019df4d8-0ed6-7834-999b-d65f5f1cb05f" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0ed6-74d9-9d05-87abf9f690b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseU.S.AgreementMember_019df4d8-0ed6-730c-944e-22ea7eb9e426" xlink:href="eols-20260630.xsd#eols_SymateseU.S.AgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0ed6-74d9-9d05-87abf9f690b8" xlink:to="loc_eols_SymateseU.S.AgreementMember_019df4d8-0ed6-730c-944e-22ea7eb9e426" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseEuropeAgreementMember_019df4d8-0ed6-794e-ae34-3e6261ef463b" xlink:href="eols-20260630.xsd#eols_SymateseEuropeAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019df4d8-0ed6-74d9-9d05-87abf9f690b8" xlink:to="loc_eols_SymateseEuropeAgreementMember_019df4d8-0ed6-794e-ae34-3e6261ef463b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneAxis_019df4d8-0ed6-7d14-b1cc-a97864e4133f" xlink:href="eols-20260630.xsd#eols_MilestoneAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LossContingenciesTable_019df4d8-0ed6-7a26-9aa0-0d975262b1bc" xlink:to="loc_eols_MilestoneAxis_019df4d8-0ed6-7d14-b1cc-a97864e4133f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneDomain_019df4d8-0ed6-7d14-b1cc-a97864e4133f_default" xlink:href="eols-20260630.xsd#eols_MilestoneDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_eols_MilestoneAxis_019df4d8-0ed6-7d14-b1cc-a97864e4133f" xlink:to="loc_eols_MilestoneDomain_019df4d8-0ed6-7d14-b1cc-a97864e4133f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MilestoneDomain_019df4d8-0ed6-7692-92f0-3ce6eef11d19" xlink:href="eols-20260630.xsd#eols_MilestoneDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_eols_MilestoneAxis_019df4d8-0ed6-7d14-b1cc-a97864e4133f" xlink:to="loc_eols_MilestoneDomain_019df4d8-0ed6-7692-92f0-3ce6eef11d19" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_FirstMilestonePaymentMember_019df4d8-0ed6-71b2-968f-22bdabe3f7d2" xlink:href="eols-20260630.xsd#eols_FirstMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_MilestoneDomain_019df4d8-0ed6-7692-92f0-3ce6eef11d19" xlink:to="loc_eols_FirstMilestonePaymentMember_019df4d8-0ed6-71b2-968f-22bdabe3f7d2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SecondMilestonePaymentMember_019df4d8-0ed6-7936-8e5a-8fe189ee1369" xlink:href="eols-20260630.xsd#eols_SecondMilestonePaymentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_MilestoneDomain_019df4d8-0ed6-7692-92f0-3ce6eef11d19" xlink:to="loc_eols_SecondMilestonePaymentMember_019df4d8-0ed6-7936-8e5a-8fe189ee1369" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PaymentsForInventory_019df4d8-0ed6-74c9-8d1d-1dbe369e192f" xlink:href="eols-20260630.xsd#eols_PaymentsForInventory"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_PaymentsForInventory_019df4d8-0ed6-74c9-8d1d-1dbe369e192f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment_019df4d8-0ed6-7e76-8445-11208d580711" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMinimumMilestonePayment_019df4d8-0ed6-7e76-8445-11208d580711" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement_019df4d8-0ed6-721e-91a9-ffc5cceedc72" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentInConnectionWithAgreement_019df4d8-0ed6-721e-91a9-ffc5cceedc72" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod_019df4d8-0ed6-72fa-81d4-113d60e8df70" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialPaymentDuePeriod_019df4d8-0ed6-72fa-81d4-113d60e8df70" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne_019df4d8-0ed6-762e-88ae-115666270223" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearOne_019df4d8-0ed6-762e-88ae-115666270223" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo_019df4d8-0ed6-7254-bda0-1e67a43f211e" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearTwo_019df4d8-0ed6-7254-bda0-1e67a43f211e" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree_019df4d8-0ed6-7095-8871-847c0ff7d811" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearThree_019df4d8-0ed6-7095-8871-847c0ff7d811" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour_019df4d8-0ed6-715c-9b34-56b049e7fbc3" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentYearFour_019df4d8-0ed6-715c-9b34-56b049e7fbc3" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7d22-bd53-6d3a2548a917" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualPaymentTriggeredRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7d22-bd53-6d3a2548a917" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense_019df4d8-0ed6-7038-b4d3-e863e2129d4f" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPaymentInProcessResearchAndDevelopmentExpense_019df4d8-0ed6-7038-b4d3-e863e2129d4f" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts_019df4d8-0ed6-7715-a96a-b44feaebd9b7" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNotAchievedNumberOfProducts_019df4d8-0ed6-7715-a96a-b44feaebd9b7" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment_019df4d8-0ed6-719e-bd8f-d4e2c9628eac" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualMilestonePayment_019df4d8-0ed6-719e-bd8f-d4e2c9628eac" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment_019df4d8-0ed6-745b-ab29-50383649510a" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfMilestonePayment_019df4d8-0ed6-745b-ab29-50383649510a" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue_019df4d8-0ed6-7fe9-9c5f-2f8c03d6f466" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementAnnualNetRevenue_019df4d8-0ed6-7fe9-9c5f-2f8c03d6f466" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7ace-830e-b60f46a8ac69" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRegulatoryApprovalNumberOfProducts_019df4d8-0ed6-7ace-830e-b60f46a8ac69" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod_019df4d8-0ed6-7a07-8272-330eeb0e4612" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMilestonePaymentPayablePeriod_019df4d8-0ed6-7a07-8272-330eeb0e4612" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent_019df4d8-0ed6-71f1-a13f-d5643da5db3f" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementLiabilityNoncurrent_019df4d8-0ed6-71f1-a13f-d5643da5db3f" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementIntangibleAsset_019df4d8-0ed6-712c-bfe4-7b3a7aacd27c" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementIntangibleAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementIntangibleAsset_019df4d8-0ed6-712c-bfe4-7b3a7aacd27c" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualAtCarryingValue_019df4d8-0ed6-738c-964a-75289364a8b5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyAccrualAtCarryingValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LossContingenciesLineItems_019df4d8-0ed6-75c3-ba29-1477011b02c7" xlink:to="loc_us-gaap_LossContingencyAccrualAtCarryingValue_019df4d8-0ed6-738c-964a-75289364a8b5" xlink:type="arc" order="18"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/StockBasedCompensationandStockholdersEquityScheduleofStockbasedCompensationExpenseDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#StockBasedCompensationandStockholdersEquityScheduleofStockbasedCompensationExpenseDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/StockBasedCompensationandStockholdersEquityScheduleofStockbasedCompensationExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019df4d8-0ed6-7311-a5ff-ab8321e9a1d0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019df4d8-0ed6-7311-a5ff-ab8321e9a1d0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementOfIncomeLocationBalanceAxis_019df4d8-0ed6-7200-934c-55955d643d34" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementOfIncomeLocationBalanceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019df4d8-0ed6-7311-a5ff-ab8321e9a1d0" xlink:to="loc_us-gaap_StatementOfIncomeLocationBalanceAxis_019df4d8-0ed6-7200-934c-55955d643d34" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_019df4d8-0ed6-7b25-a664-5547ae181e84" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_019df4d8-0ed6-7b25-a664-5547ae181e84" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0ed6-756f-90c8-e5a38a014cb1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0ed6-756f-90c8-e5a38a014cb1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount_019df4d8-0ed6-76d8-ba3d-1e260c7082fe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount_019df4d8-0ed6-76d8-ba3d-1e260c7082fe" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardCompensationCost1_019df4d8-0ed6-77e5-9b11-5ba19a2b1a60" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardCompensationCost1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardCompensationCost1_019df4d8-0ed6-77e5-9b11-5ba19a2b1a60" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/StockBasedCompensationandStockholdersEquityScheduleofStockbasedCompensationExpenseDetails_1" xlink:type="simple" xlink:href="eols-20260630.xsd#StockBasedCompensationandStockholdersEquityScheduleofStockbasedCompensationExpenseDetails_1"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/StockBasedCompensationandStockholdersEquityScheduleofStockbasedCompensationExpenseDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019df4d8-0ed6-7311-a5ff-ab8321e9a1d0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_019df4d8-0ed6-7311-a5ff-ab8321e9a1d0" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_019df4d8-0ed6-7b25-a664-5547ae181e84" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_019df4d8-0ed6-7b25-a664-5547ae181e84" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0ed6-756f-90c8-e5a38a014cb1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0ed6-756f-90c8-e5a38a014cb1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount_019df4d8-0ed6-76d8-ba3d-1e260c7082fe" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsCapitalizedAmount_019df4d8-0ed6-76d8-ba3d-1e260c7082fe" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardCompensationCost1_019df4d8-0ed6-77e5-9b11-5ba19a2b1a60" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardCompensationCost1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_019df4d8-0ed6-7c5a-83c5-579be4e915cb" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardCompensationCost1_019df4d8-0ed6-77e5-9b11-5ba19a2b1a60" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/StockBasedCompensationandStockholdersEquityNarrativeDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#StockBasedCompensationandStockholdersEquityNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/StockBasedCompensationandStockholdersEquityNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_019df4d8-0ed6-74d6-b4c4-1d2cc936c707" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_019df4d8-0ed6-74d6-b4c4-1d2cc936c707" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-71f7-acba-b3331c5e3127" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019df4d8-0ed6-74d6-b4c4-1d2cc936c707" xlink:to="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-71f7-acba-b3331c5e3127" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-71f7-acba-b3331c5e3127_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-71f7-acba-b3331c5e3127" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-71f7-acba-b3331c5e3127_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_019df4d8-0ed6-71f7-acba-b3331c5e3127" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ServiceBasedStockOptionsAndRestrictedStockUnitsMember_019df4d8-0ed6-74a2-9832-d041a3f1a615" xlink:href="eols-20260630.xsd#eols_ServiceBasedStockOptionsAndRestrictedStockUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:to="loc_eols_ServiceBasedStockOptionsAndRestrictedStockUnitsMember_019df4d8-0ed6-74a2-9832-d041a3f1a615" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockOptionMember_019df4d8-0ed6-73f7-9581-a307d31a4501" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockOptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:to="loc_us-gaap_EmployeeStockOptionMember_019df4d8-0ed6-73f7-9581-a307d31a4501" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockUnitsRSUMember_019df4d8-0ed6-7d96-aa09-39dafd1625cf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:to="loc_us-gaap_RestrictedStockUnitsRSUMember_019df4d8-0ed6-7d96-aa09-39dafd1625cf" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PerformanceRestrictedStockUnitsPRSUAndMarketBasedRestrictedStockUnitsMBRSUMember_019df4d8-0ed6-72ef-92c5-61c491921be2" xlink:href="eols-20260630.xsd#eols_PerformanceRestrictedStockUnitsPRSUAndMarketBasedRestrictedStockUnitsMBRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:to="loc_eols_PerformanceRestrictedStockUnitsPRSUAndMarketBasedRestrictedStockUnitsMBRSUMember_019df4d8-0ed6-72ef-92c5-61c491921be2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_PerformanceRestrictedStockUnitsPRSUMember_019df4d8-0ed6-7392-8ecd-0a44a0b49ec1" xlink:href="eols-20260630.xsd#eols_PerformanceRestrictedStockUnitsPRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_PerformanceRestrictedStockUnitsPRSUAndMarketBasedRestrictedStockUnitsMBRSUMember_019df4d8-0ed6-72ef-92c5-61c491921be2" xlink:to="loc_eols_PerformanceRestrictedStockUnitsPRSUMember_019df4d8-0ed6-7392-8ecd-0a44a0b49ec1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_MarketBasedRestrictedStockUnitsMBRSUMember_019df4d8-0ed6-7f1f-a59b-cc932795c5f8" xlink:href="eols-20260630.xsd#eols_MarketBasedRestrictedStockUnitsMBRSUMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_eols_PerformanceRestrictedStockUnitsPRSUAndMarketBasedRestrictedStockUnitsMBRSUMember_019df4d8-0ed6-72ef-92c5-61c491921be2" xlink:to="loc_eols_MarketBasedRestrictedStockUnitsMBRSUMember_019df4d8-0ed6-7f1f-a59b-cc932795c5f8" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_019df4d8-0ed6-7a2a-8f0c-70bdfeaa1f2e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:to="loc_us-gaap_EmployeeStockMember_019df4d8-0ed6-7a2a-8f0c-70bdfeaa1f2e" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ServiceBasedRestrictedStockUnitsMember_019df4d8-0ed6-7a37-8c12-372ed78a4c4a" xlink:href="eols-20260630.xsd#eols_ServiceBasedRestrictedStockUnitsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_019df4d8-0ed6-7c56-bbe2-ee2f7b1b3f9b" xlink:to="loc_eols_ServiceBasedRestrictedStockUnitsMember_019df4d8-0ed6-7a37-8c12-372ed78a4c4a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0ed6-7840-872f-26bf4d2be057" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsidiarySaleOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019df4d8-0ed6-74d6-b4c4-1d2cc936c707" xlink:to="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0ed6-7840-872f-26bf4d2be057" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0ed6-7840-872f-26bf4d2be057_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0ed6-7840-872f-26bf4d2be057" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0ed6-7840-872f-26bf4d2be057_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0ed6-763f-b97a-2904ce2b69e8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsidiarySaleOfStockAxis_019df4d8-0ed6-7840-872f-26bf4d2be057" xlink:to="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0ed6-763f-b97a-2904ce2b69e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ATMSalesAgreementMember_019df4d8-0ed6-744d-b6a7-c8f5633f1723" xlink:href="eols-20260630.xsd#eols_ATMSalesAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SaleOfStockNameOfTransactionDomain_019df4d8-0ed6-763f-b97a-2904ce2b69e8" xlink:to="loc_eols_ATMSalesAgreementMember_019df4d8-0ed6-744d-b6a7-c8f5633f1723" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_019df4d8-0ed6-78aa-b1c5-d2be9ea1350b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019df4d8-0ed6-74d6-b4c4-1d2cc936c707" xlink:to="loc_us-gaap_PlanNameAxis_019df4d8-0ed6-78aa-b1c5-d2be9ea1350b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_019df4d8-0ed6-78aa-b1c5-d2be9ea1350b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_019df4d8-0ed6-78aa-b1c5-d2be9ea1350b" xlink:to="loc_us-gaap_PlanNameDomain_019df4d8-0ed6-78aa-b1c5-d2be9ea1350b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_019df4d8-0ed6-7775-b1b0-bfb50c6522e5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_019df4d8-0ed6-78aa-b1c5-d2be9ea1350b" xlink:to="loc_us-gaap_PlanNameDomain_019df4d8-0ed6-7775-b1b0-bfb50c6522e5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_A2024EmployeeStockPurchasePlanMember_019df4d8-0ed6-7cc4-8e73-270f00277dc1" xlink:href="eols-20260630.xsd#eols_A2024EmployeeStockPurchasePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_019df4d8-0ed6-7775-b1b0-bfb50c6522e5" xlink:to="loc_eols_A2024EmployeeStockPurchasePlanMember_019df4d8-0ed6-7cc4-8e73-270f00277dc1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_A2023InducementIncentivePlanMember_019df4d8-0ed6-752f-bef8-7ec85b36e2c3" xlink:href="eols-20260630.xsd#eols_A2023InducementIncentivePlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_019df4d8-0ed6-7775-b1b0-bfb50c6522e5" xlink:to="loc_eols_A2023InducementIncentivePlanMember_019df4d8-0ed6-752f-bef8-7ec85b36e2c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019df4d8-0ed6-798c-9df4-307b8d39e4f0" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_019df4d8-0ed6-74d6-b4c4-1d2cc936c707" xlink:to="loc_srt_RangeAxis_019df4d8-0ed6-798c-9df4-307b8d39e4f0" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0ed6-798c-9df4-307b8d39e4f0_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019df4d8-0ed6-798c-9df4-307b8d39e4f0" xlink:to="loc_srt_RangeMember_019df4d8-0ed6-798c-9df4-307b8d39e4f0_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019df4d8-0ed6-7d05-a1d6-b13fad6b5d84" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019df4d8-0ed6-798c-9df4-307b8d39e4f0" xlink:to="loc_srt_RangeMember_019df4d8-0ed6-7d05-a1d6-b13fad6b5d84" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019df4d8-0ed6-778a-82f5-05d63465f98a" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0ed6-7d05-a1d6-b13fad6b5d84" xlink:to="loc_srt_MinimumMember_019df4d8-0ed6-778a-82f5-05d63465f98a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019df4d8-0ed6-7ee4-8207-03cdd9150d7d" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019df4d8-0ed6-7d05-a1d6-b13fad6b5d84" xlink:to="loc_srt_MaximumMember_019df4d8-0ed6-7ee4-8207-03cdd9150d7d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_019df4d8-0ed6-7306-8295-c04a4998ed55" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognized_019df4d8-0ed6-7306-8295-c04a4998ed55" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_019df4d8-0ed6-7b74-997f-44cd33c3fdbf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedPeriodForRecognition1_019df4d8-0ed6-7b74-997f-44cd33c3fdbf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesAuthorized_019df4d8-0ed6-7bb6-a529-4277f1871be7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_PreferredStockSharesAuthorized_019df4d8-0ed6-7bb6-a529-4277f1871be7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockParOrStatedValuePerShare_019df4d8-0ed6-787b-9edb-f1a6452cdbb3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_PreferredStockParOrStatedValuePerShare_019df4d8-0ed6-787b-9edb-f1a6452cdbb3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesIssued_019df4d8-0ed6-7be0-944a-2ac8b758717b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_PreferredStockSharesIssued_019df4d8-0ed6-7be0-944a-2ac8b758717b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockSharesOutstanding_019df4d8-0ed6-765d-8395-13a100421089" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_PreferredStockSharesOutstanding_019df4d8-0ed6-765d-8395-13a100421089" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesAuthorized_019df4d8-0ed6-7bfe-9d35-7776e93d2355" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_CommonStockSharesAuthorized_019df4d8-0ed6-7bfe-9d35-7776e93d2355" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockParOrStatedValuePerShare_019df4d8-0ed6-7228-af7c-03d2a0c978a1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockParOrStatedValuePerShare"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_CommonStockParOrStatedValuePerShare_019df4d8-0ed6-7228-af7c-03d2a0c978a1" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesIssued_019df4d8-0ed6-7d7f-99d0-319760e9e4a2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_CommonStockSharesIssued_019df4d8-0ed6-7d7f-99d0-319760e9e4a2" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_019df4d8-0ed6-7092-8de8-8234d6bf1215" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_019df4d8-0ed6-7092-8de8-8234d6bf1215" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_019df4d8-0ed6-7ffa-9707-edd806f2a7aa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_019df4d8-0ed6-7ffa-9707-edd806f2a7aa" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentageOfOutstandingStockMaximum_019df4d8-0ed6-7c70-998c-3ff613102c07" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentageOfOutstandingStockMaximum"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardPercentageOfOutstandingStockMaximum_019df4d8-0ed6-7c70-998c-3ff613102c07" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ShareBasedCompensationArrangementByShareBasedPaymentAwardAdditionalShareIncreaseAvailableForIssuance_019df4d8-0ed6-78fd-92f9-fe27871fe6b3" xlink:href="eols-20260630.xsd#eols_ShareBasedCompensationArrangementByShareBasedPaymentAwardAdditionalShareIncreaseAvailableForIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_eols_ShareBasedCompensationArrangementByShareBasedPaymentAwardAdditionalShareIncreaseAvailableForIssuance_019df4d8-0ed6-78fd-92f9-fe27871fe6b3" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_019df4d8-0ed6-787e-8372-6b8e02ed9db0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_019df4d8-0ed6-787e-8372-6b8e02ed9db0" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SaleOfStockMaximumConsiderationReceivable_019df4d8-0ed6-7a92-aae4-ac81ca17ffb6" xlink:href="eols-20260630.xsd#eols_SaleOfStockMaximumConsiderationReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_eols_SaleOfStockMaximumConsiderationReceivable_019df4d8-0ed6-7a92-aae4-ac81ca17ffb6" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SaleOfStockCommissionPaymentUponGrossProceedsPercentage_019df4d8-0ed6-7c57-b738-f4821cd1d1ea" xlink:href="eols-20260630.xsd#eols_SaleOfStockCommissionPaymentUponGrossProceedsPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_eols_SaleOfStockCommissionPaymentUponGrossProceedsPercentage_019df4d8-0ed6-7c57-b738-f4821cd1d1ea" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_019df4d8-0ed6-78db-ac98-0e684214e304" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_019df4d8-0ed6-78db-ac98-0e684214e304" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_019df4d8-0ed6-7f72-9f12-8b831a050519" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAuthorized_019df4d8-0ed6-7f72-9f12-8b831a050519" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_019df4d8-0ed6-7d3a-a37d-ecfd6e618cd5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNumberOfSharesAvailableForGrant_019df4d8-0ed6-7d3a-a37d-ecfd6e618cd5" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_019df4d8-0ed6-7861-bf5f-bcf6964af27f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardAwardVestingPeriod1_019df4d8-0ed6-7861-bf5f-bcf6964af27f" xlink:type="arc" order="19"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_019df4d8-0ed6-7f62-971e-f27e9b167386" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardExpirationPeriod_019df4d8-0ed6-7f62-971e-f27e9b167386" xlink:type="arc" order="20"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SharebasedCompensationArrangementbySharebasedPaymentAwardFairValueAssumptionsWeightedAverageExpectedTerm_019df4d8-0ed6-7630-94c4-49cd96015423" xlink:href="eols-20260630.xsd#eols_SharebasedCompensationArrangementbySharebasedPaymentAwardFairValueAssumptionsWeightedAverageExpectedTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_eols_SharebasedCompensationArrangementbySharebasedPaymentAwardFairValueAssumptionsWeightedAverageExpectedTerm_019df4d8-0ed6-7630-94c4-49cd96015423" xlink:type="arc" order="21"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_019df4d8-0ed6-78f0-b2f8-3ac13a98a546" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_019df4d8-0ed6-767a-b2fb-a50c7ad8b5b7" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_019df4d8-0ed6-78f0-b2f8-3ac13a98a546" xlink:type="arc" order="22"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/SegmentReportingNarrativeDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#SegmentReportingNarrativeDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/SegmentReportingNarrativeDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_019f8f73-0503-7f72-ad9f-df0d03d365cd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019f8f73-0503-76d4-914d-2209730b44e1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019f8f73-0503-7f72-ad9f-df0d03d365cd" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019f8f73-0503-76d4-914d-2209730b44e1" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TypeOfArrangementAxis_019f8f73-0503-7ad8-b8d5-90c2357f73ff" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019f8f73-0503-76d4-914d-2209730b44e1" xlink:to="loc_us-gaap_TypeOfArrangementAxis_019f8f73-0503-7ad8-b8d5-90c2357f73ff" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019f8f73-0503-7ad8-b8d5-90c2357f73ff_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019f8f73-0503-7ad8-b8d5-90c2357f73ff" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019f8f73-0503-7ad8-b8d5-90c2357f73ff_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019f8f73-0503-7319-b1cb-fdad76740b9d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_TypeOfArrangementAxis_019f8f73-0503-7ad8-b8d5-90c2357f73ff" xlink:to="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019f8f73-0503-7319-b1cb-fdad76740b9d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseEuropeAgreementMember_019f8f73-0503-707c-af09-a36945c34db1" xlink:href="eols-20260630.xsd#eols_SymateseEuropeAgreementMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ArrangementsAndNonarrangementTransactionsMember_019f8f73-0503-7319-b1cb-fdad76740b9d" xlink:to="loc_eols_SymateseEuropeAgreementMember_019f8f73-0503-707c-af09-a36945c34db1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfOperatingSegments_019df4d8-0ed6-7fe0-853a-72981a73c805" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfOperatingSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019f8f73-0503-7f72-ad9f-df0d03d365cd" xlink:to="loc_us-gaap_NumberOfOperatingSegments_019df4d8-0ed6-7fe0-853a-72981a73c805" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NumberOfReportableSegments_019df4d8-0ed6-75c1-b901-308b24e2d1ed" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NumberOfReportableSegments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019f8f73-0503-7f72-ad9f-df0d03d365cd" xlink:to="loc_us-gaap_NumberOfReportableSegments_019df4d8-0ed6-75c1-b901-308b24e2d1ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f8f73-0503-744c-9186-c1fde8f92e9c" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019f8f73-0503-7f72-ad9f-df0d03d365cd" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementNumberOfProductsLaunched_019f8f73-0503-744c-9186-c1fde8f92e9c" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/SegmentReportingScheduleofReconciliationofRevenueandSignificantSegmentItemstoNetLossDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#SegmentReportingScheduleofReconciliationofRevenueandSignificantSegmentItemstoNetLossDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/SegmentReportingScheduleofReconciliationofRevenueandSignificantSegmentItemstoNetLossDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_019df4d8-0ed6-7115-b902-22d71d00d462" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019df4d8-0ed6-795d-94b4-2a0011f64dd3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019df4d8-0ed6-7115-b902-22d71d00d462" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019df4d8-0ed6-795d-94b4-2a0011f64dd3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_019df4d8-0ed6-715a-ab4a-464a6d61827f" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019df4d8-0ed6-795d-94b4-2a0011f64dd3" xlink:to="loc_srt_ProductOrServiceAxis_019df4d8-0ed6-715a-ab4a-464a6d61827f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019df4d8-0ed6-715a-ab4a-464a6d61827f_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_019df4d8-0ed6-715a-ab4a-464a6d61827f" xlink:to="loc_srt_ProductsAndServicesDomain_019df4d8-0ed6-715a-ab4a-464a6d61827f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_019df4d8-0ed6-7506-a03f-f5f63481fced" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_019df4d8-0ed6-715a-ab4a-464a6d61827f" xlink:to="loc_srt_ProductsAndServicesDomain_019df4d8-0ed6-7506-a03f-f5f63481fced" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProductMember_019df4d8-0ed6-7bd6-801e-4ffc6b42d1b4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProductMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019df4d8-0ed6-7506-a03f-f5f63481fced" xlink:to="loc_us-gaap_ProductMember_019df4d8-0ed6-7bd6-801e-4ffc6b42d1b4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_JeuveauMember_019df4d8-0ed6-755b-81c0-39372cf2038b" xlink:href="eols-20260630.xsd#eols_JeuveauMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ProductMember_019df4d8-0ed6-7bd6-801e-4ffc6b42d1b4" xlink:to="loc_eols_JeuveauMember_019df4d8-0ed6-755b-81c0-39372cf2038b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_EvolysseMember_019df4d8-0ed6-7997-ada7-e68b68647e80" xlink:href="eols-20260630.xsd#eols_EvolysseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ProductMember_019df4d8-0ed6-7bd6-801e-4ffc6b42d1b4" xlink:to="loc_eols_EvolysseMember_019df4d8-0ed6-7997-ada7-e68b68647e80" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ServiceMember_019df4d8-0ed6-724f-bad7-ac8e46697f7d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ServiceMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_019df4d8-0ed6-7506-a03f-f5f63481fced" xlink:to="loc_us-gaap_ServiceMember_019df4d8-0ed6-724f-bad7-ac8e46697f7d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_019df4d8-0ed6-7409-9dd4-eb507e6828f5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019df4d8-0ed6-795d-94b4-2a0011f64dd3" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_019df4d8-0ed6-7409-9dd4-eb507e6828f5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_019df4d8-0ed6-7409-9dd4-eb507e6828f5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_019df4d8-0ed6-7409-9dd4-eb507e6828f5" xlink:to="loc_us-gaap_SegmentDomain_019df4d8-0ed6-7409-9dd4-eb507e6828f5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_019df4d8-0ed6-7b7d-be8a-5dde8fe36c42" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_019df4d8-0ed6-7409-9dd4-eb507e6828f5" xlink:to="loc_us-gaap_SegmentDomain_019df4d8-0ed6-7b7d-be8a-5dde8fe36c42" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_ReportableSegmentMember_019df4d8-0ed6-7b6e-8650-64b52b1100bc" xlink:href="eols-20260630.xsd#eols_ReportableSegmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_019df4d8-0ed6-7b7d-be8a-5dde8fe36c42" xlink:to="loc_eols_ReportableSegmentMember_019df4d8-0ed6-7b6e-8650-64b52b1100bc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsAxis_019df4d8-0ed6-7815-9f27-62f02ff897e4" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ConsolidationItemsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_019df4d8-0ed6-795d-94b4-2a0011f64dd3" xlink:to="loc_srt_ConsolidationItemsAxis_019df4d8-0ed6-7815-9f27-62f02ff897e4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_019df4d8-0ed6-7815-9f27-62f02ff897e4_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ConsolidationItemsAxis_019df4d8-0ed6-7815-9f27-62f02ff897e4" xlink:to="loc_srt_ConsolidationItemsDomain_019df4d8-0ed6-7815-9f27-62f02ff897e4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ConsolidationItemsDomain_019df4d8-0ed6-7662-8bdb-2ddd3e659e3b" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ConsolidationItemsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ConsolidationItemsAxis_019df4d8-0ed6-7815-9f27-62f02ff897e4" xlink:to="loc_srt_ConsolidationItemsDomain_019df4d8-0ed6-7662-8bdb-2ddd3e659e3b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_EliminationsAndReconcilingItemsMember_019df4d8-0ed6-7b79-97bc-2bd576dd1b82" xlink:href="eols-20260630.xsd#eols_EliminationsAndReconcilingItemsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ConsolidationItemsDomain_019df4d8-0ed6-7662-8bdb-2ddd3e659e3b" xlink:to="loc_eols_EliminationsAndReconcilingItemsMember_019df4d8-0ed6-7b79-97bc-2bd576dd1b82" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019df4d8-0ed6-782d-8b94-b1b0ac30d17b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019df4d8-0ed6-7115-b902-22d71d00d462" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019df4d8-0ed6-782d-8b94-b1b0ac30d17b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_019df4d8-0ed6-7115-b902-22d71d00d462" xlink:to="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_019df4d8-0ed6-73fa-b23f-34c64904182e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_019df4d8-0ed6-73fa-b23f-34c64904182e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_019df4d8-0ed6-7590-b022-74e208227272" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_GrossProfit_019df4d8-0ed6-7590-b022-74e208227272" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseExcludingAcquiredInProcessCost_019df4d8-0ed6-7ff4-aca0-26dae963c440" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResearchAndDevelopmentExpenseExcludingAcquiredInProcessCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseExcludingAcquiredInProcessCost_019df4d8-0ed6-7ff4-aca0-26dae963c440" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0ed6-72aa-acd9-5fe69145d593" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_019df4d8-0ed6-72aa-acd9-5fe69145d593" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_019df4d8-0ed6-7898-86b5-62a5c662e8ea" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_DepreciationAndAmortization_019df4d8-0ed6-7898-86b5-62a5c662e8ea" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIncomeNet_019df4d8-0ed6-72b6-9bb9-6440ea40098d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentIncomeNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_InvestmentIncomeNet_019df4d8-0ed6-72b6-9bb9-6440ea40098d" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_019df4d8-0ed6-7750-953c-5005c20ac43b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_InterestExpenseNonoperating_019df4d8-0ed6-7750-953c-5005c20ac43b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0ed6-79ba-9169-787a9d37270e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019df4d8-0ed6-79ba-9169-787a9d37270e" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingOtherItemAmount_019df4d8-0ed6-7453-bcd4-00810f7fcc07" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SegmentReportingOtherItemAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_SegmentReportingOtherItemAmount_019df4d8-0ed6-7453-bcd4-00810f7fcc07" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019df4d8-0ed6-7f6b-965c-68b552044655" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReconciliationFromSegmentTotalsToConsolidatedAbstract_019df4d8-0ed6-7261-843f-2d1f5bdad1b0" xlink:to="loc_us-gaap_NetIncomeLoss_019df4d8-0ed6-7f6b-965c-68b552044655" xlink:type="arc" order="10"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.evolus.com/role/SubsequentEventsDetails" xlink:type="simple" xlink:href="eols-20260630.xsd#SubsequentEventsDetails"/>
  <link:definitionLink xlink:role="http://www.evolus.com/role/SubsequentEventsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventLineItems_019df4d8-0ed6-7d2a-9255-e4f3fa7e7d8c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTable_019df4d8-0ed6-79c4-9fb6-238ffe86f552" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SubsequentEventLineItems_019df4d8-0ed6-7d2a-9255-e4f3fa7e7d8c" xlink:to="loc_us-gaap_SubsequentEventTable_019df4d8-0ed6-79c4-9fb6-238ffe86f552" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeAxis_019df4d8-0ed6-7c3b-a403-f40eaed5f8f2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_019df4d8-0ed6-79c4-9fb6-238ffe86f552" xlink:to="loc_us-gaap_SubsequentEventTypeAxis_019df4d8-0ed6-7c3b-a403-f40eaed5f8f2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_019df4d8-0ed6-7c3b-a403-f40eaed5f8f2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_019df4d8-0ed6-7c3b-a403-f40eaed5f8f2" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_019df4d8-0ed6-7c3b-a403-f40eaed5f8f2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventTypeDomain_019df4d8-0ed6-7745-99f9-25a6a0fc3683" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_SubsequentEventTypeAxis_019df4d8-0ed6-7c3b-a403-f40eaed5f8f2" xlink:to="loc_us-gaap_SubsequentEventTypeDomain_019df4d8-0ed6-7745-99f9-25a6a0fc3683" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubsequentEventMember_019df4d8-0ed6-7c78-803d-a09f21f931ae" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventTypeDomain_019df4d8-0ed6-7745-99f9-25a6a0fc3683" xlink:to="loc_us-gaap_SubsequentEventMember_019df4d8-0ed6-7c78-803d-a09f21f931ae" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_CounterpartyNameAxis_019f8d29-0cb8-7201-924a-4a439288e7a3" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_CounterpartyNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_SubsequentEventTable_019df4d8-0ed6-79c4-9fb6-238ffe86f552" xlink:to="loc_srt_CounterpartyNameAxis_019f8d29-0cb8-7201-924a-4a439288e7a3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_019f8d29-0cb8-7201-924a-4a439288e7a3_default" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_CounterpartyNameAxis_019f8d29-0cb8-7201-924a-4a439288e7a3" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_019f8d29-0cb8-7201-924a-4a439288e7a3_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RepurchaseAgreementCounterpartyNameDomain_019f8d29-0cb8-73e4-bf11-1d7273ee47eb" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RepurchaseAgreementCounterpartyNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_CounterpartyNameAxis_019f8d29-0cb8-7201-924a-4a439288e7a3" xlink:to="loc_srt_RepurchaseAgreementCounterpartyNameDomain_019f8d29-0cb8-73e4-bf11-1d7273ee47eb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_IBSAInstitutBiochimiqueSAMember_019f8d29-5527-78c2-9e94-3e93fe05c268" xlink:href="eols-20260630.xsd#eols_IBSAInstitutBiochimiqueSAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_019f8d29-0cb8-73e4-bf11-1d7273ee47eb" xlink:to="loc_eols_IBSAInstitutBiochimiqueSAMember_019f8d29-5527-78c2-9e94-3e93fe05c268" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_SymateseEUAmendmentsMember_019fcfde-0089-7473-9e07-1dcc8f3d99d8" xlink:href="eols-20260630.xsd#eols_SymateseEUAmendmentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RepurchaseAgreementCounterpartyNameDomain_019f8d29-0cb8-73e4-bf11-1d7273ee47eb" xlink:to="loc_eols_SymateseEUAmendmentsMember_019fcfde-0089-7473-9e07-1dcc8f3d99d8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementInitialTerm_019f8d28-5ee0-7429-8139-d3b682c92adb" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementInitialTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_019df4d8-0ed6-7d2a-9255-e4f3fa7e7d8c" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementInitialTerm_019f8d28-5ee0-7429-8139-d3b682c92adb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementRenewalTerm_019f8d2b-cb1a-7fcc-9202-8ee085d674c3" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementRenewalTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_019df4d8-0ed6-7d2a-9255-e4f3fa7e7d8c" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementRenewalTerm_019f8d2b-cb1a-7fcc-9202-8ee085d674c3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPayment_019fcde2-bbeb-7990-b625-84b3fbfac513" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementUpfrontPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_019df4d8-0ed6-7d2a-9255-e4f3fa7e7d8c" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementUpfrontPayment_019fcde2-bbeb-7990-b625-84b3fbfac513" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_eols_LicenseSupplyAndDistributionAgreementMaximumPotentialMilestonePayments_019fc68d-d5de-71e8-90e4-f3aadb4dd84e" xlink:href="eols-20260630.xsd#eols_LicenseSupplyAndDistributionAgreementMaximumPotentialMilestonePayments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SubsequentEventLineItems_019df4d8-0ed6-7d2a-9255-e4f3fa7e7d8c" xlink:to="loc_eols_LicenseSupplyAndDistributionAgreementMaximumPotentialMilestonePayments_019fc68d-d5de-71e8-90e4-f3aadb4dd84e" xlink:type="arc" order="3"/>
  </link:definitionLink>
</link:linkbase>
