<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019e03dc-5507-7f45-b951-0d9d22ecae36,g:54ae101d-ac40-48b0-a514-870a05715f4f-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="https://xbrl.org/2023/arcrole/summation-item" xlink:type="simple" xlink:href="https://www.xbrl.org/2023/calculation-1.1.xsd#summation-item"/>
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDINCOMESTATEMENTSUnaudited" xlink:type="simple" xlink:href="csx-20260630.xsd#CONSOLIDATEDINCOMESTATEMENTSUnaudited"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDINCOMESTATEMENTSUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019e03dc-55de-73bf-8048-9fff03a7cbd9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e03dc-55de-73b9-92d3-8de6a5e4d4aa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_019e03dc-55de-73bf-8048-9fff03a7cbd9" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019e03dc-55de-73b9-92d3-8de6a5e4d4aa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-795f-848b-204679c48b21" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingCostsAndExpenses"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_019e03dc-55de-73bf-8048-9fff03a7cbd9" xlink:to="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-795f-848b-204679c48b21" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e03dc-55de-749c-9152-33031fc152fd" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e03dc-55de-7e32-92cc-123651b801be" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_019e03dc-55de-749c-9152-33031fc152fd" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e03dc-55de-7e32-92cc-123651b801be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_019e03dc-55de-7ac6-8437-efa611286e79" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_019e03dc-55de-749c-9152-33031fc152fd" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_019e03dc-55de-7ac6-8437-efa611286e79" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-764c-bbaa-209f2cb0353c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingCostsAndExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LaborAndRelatedExpense_019e03dc-55de-7300-b809-0b41476ae2d4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LaborAndRelatedExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-764c-bbaa-209f2cb0353c" xlink:to="loc_us-gaap_LaborAndRelatedExpense_019e03dc-55de-7300-b809-0b41476ae2d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCostAndExpenseOperating_019e03dc-55de-7736-b32f-a5262a173b27" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherCostAndExpenseOperating"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-764c-bbaa-209f2cb0353c" xlink:to="loc_us-gaap_OtherCostAndExpenseOperating_019e03dc-55de-7736-b32f-a5262a173b27" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_019e03dc-55de-7b76-8d52-3803d2e185f6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-764c-bbaa-209f2cb0353c" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_019e03dc-55de-7b76-8d52-3803d2e185f6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FuelCosts_019e03dc-55de-78d4-93b2-11d2894e1e56" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FuelCosts"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-764c-bbaa-209f2cb0353c" xlink:to="loc_us-gaap_FuelCosts_019e03dc-55de-78d4-93b2-11d2894e1e56" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_EquipmentAndOtherRents_019e03dc-55de-73b1-aaf9-578e3d2d95bd" xlink:href="csx-20260630.xsd#csx_EquipmentAndOtherRents"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingCostsAndExpenses_019e03dc-55de-764c-bbaa-209f2cb0353c" xlink:to="loc_csx_EquipmentAndOtherRents_019e03dc-55de-73b1-aaf9-578e3d2d95bd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e03dc-55de-76c4-a602-5ec56bda3036" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019e03dc-55de-79d8-b7d9-884a68e3863a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e03dc-55de-76c4-a602-5ec56bda3036" xlink:to="loc_us-gaap_OperatingIncomeLoss_019e03dc-55de-79d8-b7d9-884a68e3863a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_019e03dc-55de-7984-afb7-4df69190924f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e03dc-55de-76c4-a602-5ec56bda3036" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_019e03dc-55de-7984-afb7-4df69190924f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_019e03dc-55de-7c3c-ba4c-6701568c52e0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_019e03dc-55de-76c4-a602-5ec56bda3036" xlink:to="loc_us-gaap_InterestExpenseNonoperating_019e03dc-55de-7c3c-ba4c-6701568c52e0" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="simple" xlink:href="csx-20260630.xsd#CONSOLIDATEDBALANCESHEETS"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDBALANCESHEETS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_019e03dc-55de-7e0f-86b0-77228de85d0f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_019e03dc-55de-79d9-8196-d3a3ea8c53ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019e03dc-55de-7e0f-86b0-77228de85d0f" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_019e03dc-55de-79d9-8196-d3a3ea8c53ec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermInvestments_019e03dc-55de-787c-8ef8-34482e4908de" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShortTermInvestments"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019e03dc-55de-7e0f-86b0-77228de85d0f" xlink:to="loc_us-gaap_ShortTermInvestments_019e03dc-55de-787c-8ef8-34482e4908de" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_019e03dc-55de-70d2-b5a1-6ff91169be90" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019e03dc-55de-7e0f-86b0-77228de85d0f" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_019e03dc-55de-70d2-b5a1-6ff91169be90" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MaterialsSuppliesAndOther_019e03dc-55de-7844-862d-ec203a7f5b5b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MaterialsSuppliesAndOther"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019e03dc-55de-7e0f-86b0-77228de85d0f" xlink:to="loc_us-gaap_MaterialsSuppliesAndOther_019e03dc-55de-7844-862d-ec203a7f5b5b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_019e03dc-55de-755a-8566-e3f7f081ad64" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherAssetsCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019e03dc-55de-7e0f-86b0-77228de85d0f" xlink:to="loc_us-gaap_OtherAssetsCurrent_019e03dc-55de-755a-8566-e3f7f081ad64" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7d4a-b570-18332dc2ed13" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MinorityInterest_019e03dc-55de-7ed7-902b-4beef22c3cb7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MinorityInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7d4a-b570-18332dc2ed13" xlink:to="loc_us-gaap_MinorityInterest_019e03dc-55de-7ed7-902b-4beef22c3cb7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_019e03dc-55de-7416-92bf-bc93664e33f6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7d4a-b570-18332dc2ed13" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_019e03dc-55de-7416-92bf-bc93664e33f6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_019e03dc-55de-77e3-b693-66b4761d56d8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7d4a-b570-18332dc2ed13" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_019e03dc-55de-77e3-b693-66b4761d56d8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalCommonStock_019e03dc-55de-7d26-9597-caa9a5b214cb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalCommonStock"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7d4a-b570-18332dc2ed13" xlink:to="loc_us-gaap_AdditionalPaidInCapitalCommonStock_019e03dc-55de-7d26-9597-caa9a5b214cb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_019e03dc-55de-7f2a-ad96-4d787ea5303b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7d4a-b570-18332dc2ed13" xlink:to="loc_us-gaap_CommonStockValue_019e03dc-55de-7f2a-ad96-4d787ea5303b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_019e03dc-55de-75aa-97d8-978ad7cf57b2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7670-95ff-82557d21e842" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_019e03dc-55de-75aa-97d8-978ad7cf57b2" xlink:to="loc_us-gaap_StockholdersEquityIncludingPortionAttributableToNoncontrollingInterest_019e03dc-55de-7670-95ff-82557d21e842" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_019e03dc-55de-7b69-a682-eb3cde59fc70" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_019e03dc-55de-75aa-97d8-978ad7cf57b2" xlink:to="loc_us-gaap_Liabilities_019e03dc-55de-7b69-a682-eb3cde59fc70" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetIncludingGoodwill_019e03dc-55de-7c21-9d85-da5f75922fa9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IntangibleAssetsNetIncludingGoodwill"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:to="loc_us-gaap_IntangibleAssetsNetIncludingGoodwill_019e03dc-55de-7c21-9d85-da5f75922fa9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_019e03dc-55de-75c9-93e1-5c5b818a54bb" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:to="loc_us-gaap_AssetsCurrent_019e03dc-55de-75c9-93e1-5c5b818a54bb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_019e03dc-55de-7264-8abf-d5914d20ad36" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_019e03dc-55de-7264-8abf-d5914d20ad36" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_019e03dc-55de-71ba-8f64-10703038bd76" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_019e03dc-55de-71ba-8f64-10703038bd76" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_019e03dc-55de-7803-b1b9-2ed145bcb45a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:to="loc_us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_019e03dc-55de-7803-b1b9-2ed145bcb45a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_019e03dc-55de-7cd0-928b-3a3e322ccb75" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019e03dc-55de-7151-b052-621b58d96ce2" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_019e03dc-55de-7cd0-928b-3a3e322ccb75" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_019e03dc-55de-7222-88dd-663acb0f84ac" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_019e03dc-55de-748b-be85-2cf8813f624f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_019e03dc-55de-7222-88dd-663acb0f84ac" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_019e03dc-55de-748b-be85-2cf8813f624f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_019e03dc-55de-73b3-bee5-a671ab71c9a5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_019e03dc-55de-7222-88dd-663acb0f84ac" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_019e03dc-55de-73b3-bee5-a671ab71c9a5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_019e03dc-55de-7711-8680-7d8cb0926c5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_AccountsPayableCurrent_019e03dc-55de-7711-8680-7d8cb0926c5c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_019e03dc-55de-79a4-b055-628351613ae2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_019e03dc-55de-79a4-b055-628351613ae2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_019e03dc-55de-7b9e-81d0-5a4d34037eb2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyAccrualCarryingValueCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_019e03dc-55de-7b9e-81d0-5a4d34037eb2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_019e03dc-55de-7c80-8f9c-178c41df75af" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_019e03dc-55de-7c80-8f9c-178c41df75af" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_019e03dc-55de-729b-9d0b-ef4cef5c8985" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_TaxesPayableCurrent_019e03dc-55de-729b-9d0b-ef4cef5c8985" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestPayableCurrent_019e03dc-55de-77d6-b558-ba0fed743ae9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestPayableCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_InterestPayableCurrent_019e03dc-55de-77d6-b558-ba0fed743ae9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_019e03dc-55de-74ae-b642-ab13393e6f22" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7f48-9593-a23bb77c7824" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_019e03dc-55de-74ae-b642-ab13393e6f22" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_019e03dc-55de-70d2-b8ed-fda3fc503e47" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyAccrualCarryingValueNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_019e03dc-55de-70d2-b8ed-fda3fc503e47" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_019e03dc-55de-7838-832a-7f6c56043c2d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_019e03dc-55de-7838-832a-7f6c56043c2d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_019e03dc-55de-747d-9964-6da4ab86a263" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligations"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_019e03dc-55de-747d-9964-6da4ab86a263" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_019e03dc-55de-7afb-8730-464fec9bb1e9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_019e03dc-55de-7afb-8730-464fec9bb1e9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_019e03dc-55de-7c46-98ce-cf37c2b868a3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DeferredIncomeTaxLiabilitiesNet"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilitiesNet_019e03dc-55de-7c46-98ce-cf37c2b868a3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7015-88cf-a3b191d8b37c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019e03dc-55de-7cca-9e82-453d20c089bf" xlink:to="loc_us-gaap_LiabilitiesCurrent_019e03dc-55de-7015-88cf-a3b191d8b37c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDCASHFLOWSTATEMENTSUnaudited" xlink:type="simple" xlink:href="csx-20260630.xsd#CONSOLIDATEDCASHFLOWSTATEMENTSUnaudited"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDCASHFLOWSTATEMENTSUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-75d2-a1de-adeb246947f0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_019e03dc-55de-76d7-8b67-78287fcbf8d9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsForRepurchaseOfCommonStock"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-75d2-a1de-adeb246947f0" xlink:to="loc_us-gaap_PaymentsForRepurchaseOfCommonStock_019e03dc-55de-76d7-8b67-78287fcbf8d9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDividendsCommonStock_019e03dc-55de-7c4c-9335-fb3a28d0a34d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsOfDividendsCommonStock"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-75d2-a1de-adeb246947f0" xlink:to="loc_us-gaap_PaymentsOfDividendsCommonStock_019e03dc-55de-7c4c-9335-fb3a28d0a34d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RepaymentsOfLongTermDebt_019e03dc-55de-791f-a70f-94cdec261bf6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RepaymentsOfLongTermDebt"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-75d2-a1de-adeb246947f0" xlink:to="loc_us-gaap_RepaymentsOfLongTermDebt_019e03dc-55de-791f-a70f-94cdec261bf6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_019e03dc-55de-760c-85c2-116f80ac38e9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfLongTermDebt"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-75d2-a1de-adeb246947f0" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfLongTermDebt_019e03dc-55de-760c-85c2-116f80ac38e9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_019e03dc-55de-7496-8ef5-a556706cf35d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromPaymentsForOtherFinancingActivities"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-75d2-a1de-adeb246947f0" xlink:to="loc_us-gaap_ProceedsFromPaymentsForOtherFinancingActivities_019e03dc-55de-7496-8ef5-a556706cf35d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_019e03dc-55de-73b6-8c2b-f1a278648c37" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_019e03dc-55de-73b6-8c2b-f1a278648c37" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireShortTermInvestments_019e03dc-55de-7d3a-883a-423b2292931c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquireShortTermInvestments"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:to="loc_us-gaap_PaymentsToAcquireShortTermInvestments_019e03dc-55de-7d3a-883a-423b2292931c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfShortTermInvestments_019e3aca-3495-701a-8905-cd204139db38" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromSaleOfShortTermInvestments"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:to="loc_us-gaap_ProceedsFromSaleOfShortTermInvestments_019e3aca-3495-701a-8905-cd204139db38" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_019e03dc-55de-75fe-90db-ffc64c7a406f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:to="loc_us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment_019e03dc-55de-75fe-90db-ffc64c7a406f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_019e03dc-55de-7715-b3f8-5f56d91905ee" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:to="loc_us-gaap_PaymentsToAcquireBusinessesNetOfCashAcquired_019e03dc-55de-7715-b3f8-5f56d91905ee" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsForProceedsFromOtherInvestingActivities_019e03dc-55de-76a1-80ab-648864be844b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsForProceedsFromOtherInvestingActivities"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7b3e-9522-e09e69f63f47" xlink:to="loc_us-gaap_PaymentsForProceedsFromOtherInvestingActivities_019e03dc-55de-76a1-80ab-648864be844b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019e03dc-55de-7b04-8c0a-6a8706bdab31" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-7c97-9cd6-6ff0e0605c19" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019e03dc-55de-7b04-8c0a-6a8706bdab31" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019e03dc-55de-7c97-9cd6-6ff0e0605c19" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7144-906b-01a2e8de4389" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019e03dc-55de-7b04-8c0a-6a8706bdab31" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019e03dc-55de-7144-906b-01a2e8de4389" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-7b24-899d-59aff10070ec" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019e03dc-55de-7b04-8c0a-6a8706bdab31" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-7b24-899d-59aff10070ec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e03dc-55de-7b9b-8244-69a2c297e524" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_NetIncomeLoss_019e03dc-55de-7b9b-8244-69a2c297e524" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationDepletionAndAmortization_019e03dc-55de-7168-a576-3c9c711b0511" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DepreciationDepletionAndAmortization"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_DepreciationDepletionAndAmortization_019e03dc-55de-7168-a576-3c9c711b0511" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_019e03dc-55de-7962-be13-677c71c7e9e5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DeferredIncomeTaxExpenseBenefit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_DeferredIncomeTaxExpenseBenefit_019e03dc-55de-7962-be13-677c71c7e9e5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_019e03dc-55de-7363-9c8c-10ab842b4d77" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherOperatingActivitiesCashFlowStatement"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_OtherOperatingActivitiesCashFlowStatement_019e03dc-55de-7363-9c8c-10ab842b4d77" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_019e03dc-55de-72f0-8533-f90ba271f60a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_019e03dc-55de-72f0-8533-f90ba271f60a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherCurrentAssets_019e03dc-55de-7011-bc2b-0ff249bf54db" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInOtherCurrentAssets"/>
    <link:calculationArc order="6" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherCurrentAssets_019e03dc-55de-7011-bc2b-0ff249bf54db" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_019e03dc-55de-7139-8a0d-b689c88cca54" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_019e03dc-55de-7139-8a0d-b689c88cca54" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedTaxesPayable_019e03dc-55de-7bfd-b201-3167d3e6777a" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInAccruedTaxesPayable"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedTaxesPayable_019e03dc-55de-7bfd-b201-3167d3e6777a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOtherCurrentLiabilities_019e03dc-55de-73f1-90a4-68d7e9a78513" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInOtherCurrentLiabilities"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019e03dc-55de-79ac-b929-da87f2067568" xlink:to="loc_us-gaap_IncreaseDecreaseInOtherCurrentLiabilities_019e03dc-55de-73f1-90a4-68d7e9a78513" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CONSOLIDATEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITYUnaudited" xlink:type="simple" xlink:href="csx-20260630.xsd#CONSOLIDATEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITYUnaudited"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CONSOLIDATEDSTATEMENTSOFCHANGESINSHAREHOLDERSEQUITYUnaudited" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_019e03dc-55de-76f2-a2ba-478776f94087" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_019e03dc-55de-7fdb-89c0-c93186fd3c87" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_019e03dc-55de-76f2-a2ba-478776f94087" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTaxPortionAttributableToParent_019e03dc-55de-7fdb-89c0-c93186fd3c87" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019e03dc-55de-7e7a-8632-06b5702ee440" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_019e03dc-55de-76f2-a2ba-478776f94087" xlink:to="loc_us-gaap_NetIncomeLoss_019e03dc-55de-7e7a-8632-06b5702ee440" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/EarningsPerShareScheduleofComputationofBasicandDilutedEarningsperShareDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#EarningsPerShareScheduleofComputationofBasicandDilutedEarningsperShareDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/EarningsPerShareScheduleofComputationofBasicandDilutedEarningsperShareDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019e03dc-56ad-788a-aa1a-36dd65fb5cb4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019e03dc-56ad-73f0-9f09-dd2f443beae4" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019e03dc-56ad-788a-aa1a-36dd65fb5cb4" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019e03dc-56ad-73f0-9f09-dd2f443beae4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_019e03dc-56ad-7506-b80f-08cef7a317f7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019e03dc-56ad-788a-aa1a-36dd65fb5cb4" xlink:to="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_019e03dc-56ad-7506-b80f-08cef7a317f7" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/CasualtyEnvironmentalandOtherReservesScheduleofBalanceSheetPresentationDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#CasualtyEnvironmentalandOtherReservesScheduleofBalanceSheetPresentationDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/CasualtyEnvironmentalandOtherReservesScheduleofBalanceSheetPresentationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualAtCarryingValue_019e03dc-56ad-7d0b-9832-ad2c8d249baa" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyAccrualAtCarryingValue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_019e03dc-56ad-7266-b959-66cc16495816" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyAccrualCarryingValueCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LossContingencyAccrualAtCarryingValue_019e03dc-56ad-7d0b-9832-ad2c8d249baa" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueCurrent_019e03dc-56ad-7266-b959-66cc16495816" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_019e03dc-56ad-700a-97a9-8a7648b4ef69" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingencyAccrualCarryingValueNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LossContingencyAccrualAtCarryingValue_019e03dc-56ad-7d0b-9832-ad2c8d249baa" xlink:to="loc_us-gaap_LossContingencyAccrualCarryingValueNoncurrent_019e03dc-56ad-700a-97a9-8a7648b4ef69" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/EmployeeBenefitPlansScheduleofComponentsofNetPeriodicBenefitCostsDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#EmployeeBenefitPlansScheduleofComponentsofNetPeriodicBenefitCostsDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/EmployeeBenefitPlansScheduleofComponentsofNetPeriodicBenefitCostsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_019e03dc-56ad-783e-8964-eca017beef6d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanInterestCost_019e03dc-56ad-7b8d-9cb2-c0a137b61aa3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DefinedBenefitPlanInterestCost"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_019e03dc-56ad-783e-8964-eca017beef6d" xlink:to="loc_us-gaap_DefinedBenefitPlanInterestCost_019e03dc-56ad-7b8d-9cb2-c0a137b61aa3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_019e03dc-56ad-7cf9-b66c-7e2ef5bc918f" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_019e03dc-56ad-783e-8964-eca017beef6d" xlink:to="loc_us-gaap_DefinedBenefitPlanExpectedReturnOnPlanAssets_019e03dc-56ad-7cf9-b66c-7e2ef5bc918f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses_019e03dc-56ad-7816-9c62-2498ad42c909" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_019e03dc-56ad-783e-8964-eca017beef6d" xlink:to="loc_us-gaap_DefinedBenefitPlanAmortizationOfGainsLosses_019e03dc-56ad-7816-9c62-2498ad42c909" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_019e03dc-56ad-7078-8ca5-6c17abf0df5c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_019e03dc-56ad-758a-bad4-b23ddfa90f61" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_019e03dc-56ad-7078-8ca5-6c17abf0df5c" xlink:to="loc_us-gaap_NetPeriodicDefinedBenefitsExpenseReversalOfExpenseExcludingServiceCostComponent_019e03dc-56ad-758a-bad4-b23ddfa90f61" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanServiceCost_019e03dc-56ad-779f-af42-c40fb8ece917" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DefinedBenefitPlanServiceCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_DefinedBenefitPlanNetPeriodicBenefitCost_019e03dc-56ad-7078-8ca5-6c17abf0df5c" xlink:to="loc_us-gaap_DefinedBenefitPlanServiceCost_019e03dc-56ad-779f-af42-c40fb8ece917" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/DebtandCreditAgreementsScheduleofActivityRelatedtoLongTermDebtDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#DebtandCreditAgreementsScheduleofActivityRelatedtoLongTermDebtDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/DebtandCreditAgreementsScheduleofActivityRelatedtoLongTermDebtDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtAndCapitalLeaseObligations_019e03dc-56ad-7e7e-906d-26a7f93d0d0b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtAndCapitalLeaseObligations"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_019e03dc-56ad-7c8c-9dcb-4aa211c4fac1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_DebtAndCapitalLeaseObligations_019e03dc-56ad-7e7e-906d-26a7f93d0d0b" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligationsCurrent_019e03dc-56ad-7c8c-9dcb-4aa211c4fac1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_019e03dc-56ad-7835-b8ce-56312b0c6552" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtAndCapitalLeaseObligations"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_DebtAndCapitalLeaseObligations_019e03dc-56ad-7e7e-906d-26a7f93d0d0b" xlink:to="loc_us-gaap_LongTermDebtAndCapitalLeaseObligations_019e03dc-56ad-7835-b8ce-56312b0c6552" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/RevenuesScheduleofAccountsReceivableNetDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#RevenuesScheduleofAccountsReceivableNetDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/RevenuesScheduleofAccountsReceivableNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNet_019e03dc-56ae-7ae5-b1b1-c49f9765e5b9" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsReceivableNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableGross_019e03dc-56ae-7a92-8dd0-d40deb6de30c" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsReceivableGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsReceivableNet_019e03dc-56ae-7ae5-b1b1-c49f9765e5b9" xlink:to="loc_us-gaap_AccountsReceivableGross_019e03dc-56ae-7a92-8dd0-d40deb6de30c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_019e03dc-56ae-78f4-91e3-c0e6271f21b8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AccountsReceivableNet_019e03dc-56ae-7ae5-b1b1-c49f9765e5b9" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_019e03dc-56ae-78f4-91e3-c0e6271f21b8" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/FairValueMeasurementsScheduleofInvestmentMaturitiesDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#FairValueMeasurementsScheduleofInvestmentMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/FairValueMeasurementsScheduleofInvestmentMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentsFairValueDisclosure_019f896c-7877-77fe-83fd-c54135385cc7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentsFairValueDisclosure"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_InvestmentsMaturityAllocatedAndSingleMaturityDateRollingWithinOneYearFairValue_019f896c-7877-7bd8-9363-5e41e910a385" xlink:href="csx-20260630.xsd#csx_InvestmentsMaturityAllocatedAndSingleMaturityDateRollingWithinOneYearFairValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_019f896c-7877-77fe-83fd-c54135385cc7" xlink:to="loc_csx_InvestmentsMaturityAllocatedAndSingleMaturityDateRollingWithinOneYearFairValue_019f896c-7877-7bd8-9363-5e41e910a385" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_InvestmentsFairValueMaturityAllocatedAndSingleMaturityDateAfterYearOneThroughFive_019f896c-7877-7bb2-89ef-3914054da779" xlink:href="csx-20260630.xsd#csx_InvestmentsFairValueMaturityAllocatedAndSingleMaturityDateAfterYearOneThroughFive"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_019f896c-7877-77fe-83fd-c54135385cc7" xlink:to="loc_csx_InvestmentsFairValueMaturityAllocatedAndSingleMaturityDateAfterYearOneThroughFive_019f896c-7877-7bb2-89ef-3914054da779" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_InvestmentsFairValueMaturityAllocatedAndSingleMaturityDateAfterYear5Through10_019f896c-7877-7f82-86d9-6cdc39fc82bf" xlink:href="csx-20260630.xsd#csx_InvestmentsFairValueMaturityAllocatedAndSingleMaturityDateAfterYear5Through10"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_019f896c-7877-77fe-83fd-c54135385cc7" xlink:to="loc_csx_InvestmentsFairValueMaturityAllocatedAndSingleMaturityDateAfterYear5Through10_019f896c-7877-7f82-86d9-6cdc39fc82bf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_InvestmentsMaturityAllocatedAndSingleMaturityDateRollingAfter10YearsFairValue_019f896c-7877-7bf1-9edd-68a326892c83" xlink:href="csx-20260630.xsd#csx_InvestmentsMaturityAllocatedAndSingleMaturityDateRollingAfter10YearsFairValue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_InvestmentsFairValueDisclosure_019f896c-7877-77fe-83fd-c54135385cc7" xlink:to="loc_csx_InvestmentsMaturityAllocatedAndSingleMaturityDateRollingAfter10YearsFairValue_019f896c-7877-7bf1-9edd-68a326892c83" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/OtherComprehensiveIncomeLossScheduleofChangesinAOCIbalancebyComponentsDetails" xlink:type="simple" xlink:href="csx-20260630.xsd#OtherComprehensiveIncomeLossScheduleofChangesinAOCIbalancebyComponentsDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/OtherComprehensiveIncomeLossScheduleofChangesinAOCIbalancebyComponentsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_019e03dc-56ae-7433-8659-768fbe0326d6" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodBeforeTax_019e03dc-56ae-75fe-a017-81ce65b20471" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodBeforeTax"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_019e03dc-56ae-7433-8659-768fbe0326d6" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodBeforeTax_019e03dc-56ae-75fe-a017-81ce65b20471" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAociCurrentPeriodTax_019e03dc-56ae-7df0-9714-3e7992669050" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ReclassificationFromAociCurrentPeriodTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_019e03dc-56ae-7433-8659-768fbe0326d6" xlink:to="loc_us-gaap_ReclassificationFromAociCurrentPeriodTax_019e03dc-56ae-7df0-9714-3e7992669050" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
