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          <link:definition>999058 - Disclosure - Organization (Details Narrative)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/PreferredStockDetailsNarrative" id="PreferredStockDetailsNarrative">
          <link:definition>999059 - Disclosure - Preferred Stock (Details Narrative)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/ScheduleOfGrant-dateFairValueOfStockOptionsDetails" id="ScheduleOfGrant-dateFairValueOfStockOptionsDetails">
          <link:definition>999060 - Disclosure - Schedule of Grant-Date Fair Value of Stock Options (Details)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/ScheduleOfIncomeTaxBenefitDetails" id="ScheduleOfIncomeTaxBenefitDetails">
          <link:definition>999061 - Disclosure - Schedule of Income Tax Benefit (Details)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/ScheduleOfIncomeTaxesPaidDetails" id="ScheduleOfIncomeTaxesPaidDetails">
          <link:definition>999062 - Disclosure - Schedule of Income Taxes Paid (Details)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/GoingConcernDetailsNarrative" id="GoingConcernDetailsNarrative">
          <link:definition>999063 - Disclosure - Going Concern (Details Narrative)</link:definition>
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          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
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        <link:roleType roleURI="http://pulmatrix.com/role/RelatedPartyTransactionsDetailsNarrative" id="RelatedPartyTransactionsDetailsNarrative">
          <link:definition>999064 - Disclosure - Related Party Transactions (Details Narrative)</link:definition>
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          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/SubsequentEventsDetailsNarrative" id="SubsequentEventsDetailsNarrative">
          <link:definition>999065 - Disclosure - Subsequent Events (Details Narrative)</link:definition>
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          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/ScheduleOfDeferredAssetsDetails" id="ScheduleOfDeferredAssetsDetails">
          <link:definition>999066 - Disclosure - Schedule of Deferred Assets (Details)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/ScheduleOfPrepaidExpensesDetails" id="ScheduleOfPrepaidExpensesDetails">
          <link:definition>999067 - Disclosure - Schedule of Prepaid Expenses (Details)</link:definition>
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          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
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        <link:roleType roleURI="http://pulmatrix.com/role/ScheduleOfAccruedExpensesDetails" id="ScheduleOfAccruedExpensesDetails">
          <link:definition>999068 - Disclosure - Schedule of Accrued Expenses (Details)</link:definition>
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          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/ShortTermLoanDetailsNarrative" id="ShortTermLoanDetailsNarrative">
          <link:definition>999069 - Disclosure - Short Term Loan (Details Narrative)</link:definition>
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          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
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        <link:roleType roleURI="http://pulmatrix.com/role/ProposedMergerAgreementDetailsNarrative" id="ProposedMergerAgreementDetailsNarrative">
          <link:definition>999070 - Disclosure - Proposed Merger Agreement (Details Narrative)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
        </link:roleType>
        <link:roleType roleURI="http://pulmatrix.com/role/BackstopFinancingAgreementDetailsNarrative" id="BackstopFinancingAgreementDetailsNarrative">
          <link:definition>999071 - Disclosure - Backstop Financing Agreement (Details Narrative)</link:definition>
          <link:usedOn>link:presentationLink</link:usedOn>
          <link:usedOn>link:calculationLink</link:usedOn>
          <link:usedOn>link:definitionLink</link:usedOn>
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        <link:linkbaseRef xlink:type="simple" xlink:href="pulm-20260331_pre.xml" xlink:role="http://www.xbrl.org/2003/role/presentationLinkbaseRef" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:title="Presentation Links" />
        <link:linkbaseRef xlink:type="simple" xlink:href="pulm-20260331_lab.xml" xlink:role="http://www.xbrl.org/2003/role/labelLinkbaseRef" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:title="Label Links" />
        <link:linkbaseRef xlink:type="simple" xlink:href="pulm-20260331_cal.xml" xlink:role="http://www.xbrl.org/2003/role/calculationLinkbaseRef" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:title="Calculation Links" />
        <link:linkbaseRef xlink:type="simple" xlink:href="pulm-20260331_def.xml" xlink:role="http://www.xbrl.org/2003/role/definitionLinkbaseRef" xlink:arcrole="http://www.w3.org/1999/xlink/properties/linkbase" xlink:title="Definition Links" />
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    <element id="PULM_ScheduleofStockbasedCompensationExpensesAbstract" name="ScheduleofStockbasedCompensationExpensesAbstract" abstract="true" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_ScheduleofStockbasedCompensationExpensesTable" name="ScheduleofStockbasedCompensationExpensesTable" abstract="true" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrldt:hypercubeItem" />
    <element id="PULM_CommonStockCapitalSharesReserved" name="CommonStockCapitalSharesReserved" nillable="true" xbrli:periodType="instant" type="xbrli:sharesItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingWeightedAverageRemainingContractualTerm" name="SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingWeightedAverageRemainingContractualTerm" nillable="true" xbrli:periodType="duration" type="xbrli:durationItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_EffectiveIncomeTaxRateReconciliationNondeductibleExpensePermanentDifferences" name="EffectiveIncomeTaxRateReconciliationNondeductibleExpensePermanentDifferences" nillable="true" xbrli:periodType="duration" type="dtr-types:percentItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_EffectiveIncomeTaxReconciliationLimitationsOnCreditAndNetOperatingLosses" name="EffectiveIncomeTaxReconciliationLimitationsOnCreditAndNetOperatingLosses" nillable="true" xbrli:periodType="duration" type="dtr-types:percentItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_DeferredTaxAssetsDeferredExpenseCapitalizedResearchAndDevelopment" name="DeferredTaxAssetsDeferredExpenseCapitalizedResearchAndDevelopment" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_DeferredTaxAssetsDeferredExpenseCapitalizedStartup" name="DeferredTaxAssetsDeferredExpenseCapitalizedStartup" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_DeferredTaxAssetsChangeValuationAllowance" name="DeferredTaxAssetsChangeValuationAllowance" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_ProceedsForWorkingCapital" name="ProceedsForWorkingCapital" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_RelatedPartyTransactionsTable" name="RelatedPartyTransactionsTable" abstract="true" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrldt:hypercubeItem" />
    <element id="PULM_LicensePayments" name="LicensePayments" nillable="true" xbrli:periodType="duration" xbrli:balance="debit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_MergerAgreementsDescriptionAndTerms" name="MergerAgreementsDescriptionAndTerms" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_FinancingFees" name="FinancingFees" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_AgreementsDescription" name="AgreementsDescription" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_LegalRetainerFees" name="LegalRetainerFees" nillable="true" xbrli:periodType="instant" xbrli:balance="debit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_ExternalResearchAndDevelopmentExpenses" name="ExternalResearchAndDevelopmentExpenses" nillable="true" xbrli:periodType="instant" xbrli:balance="credit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_BackstopFinancingAgreementAbstract" name="BackstopFinancingAgreementAbstract" abstract="true" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_BackstopFinancingAgreementTable" name="BackstopFinancingAgreementTable" abstract="true" nillable="true" xbrli:periodType="duration" type="xbrli:stringItemType" substitutionGroup="xbrldt:hypercubeItem" />
    <element id="PULM_AmountTransferredForNotes" name="AmountTransferredForNotes" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
    <element id="PULM_AggregateCommonStockValueOriginationFee" name="AggregateCommonStockValueOriginationFee" nillable="true" xbrli:periodType="duration" xbrli:balance="credit" type="xbrli:monetaryItemType" substitutionGroup="xbrli:item" />
</schema>
