<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019d2d09-be5d-7220-b3b1-f9db32257800,g:573d860d-3e71-4e58-adb0-feb04a435320-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="https://xbrl.org/2023/arcrole/summation-item" xlink:type="simple" xlink:href="https://www.xbrl.org/2023/calculation-1.1.xsd#summation-item"/>
  <link:roleRef roleURI="http://www.peoplesbancorp.com/role/StatementsofNetAssetsAvailableforBenefits" xlink:type="simple" xlink:href="pebo-20260630.xsd#StatementsofNetAssetsAvailableforBenefits"/>
  <link:calculationLink xlink:role="http://www.peoplesbancorp.com/role/StatementsofNetAssetsAvailableforBenefits" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d3258-f48a-78c9-b6bc-d7eebcc0cdf1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue_019d3258-f48a-715f-9953-3cedcd4b3f6e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d3258-f48a-78c9-b6bc-d7eebcc0cdf1" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue_019d3258-f48a-715f-9953-3cedcd4b3f6e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionReceivable_019d3258-f48a-7d5c-a491-c34b45337315" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerContributionReceivable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d3258-f48a-78c9-b6bc-d7eebcc0cdf1" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionReceivable_019d3258-f48a-7d5c-a491-c34b45337315" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant_019d3258-f48a-791b-b255-08814cba75d4" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d3258-f48a-78c9-b6bc-d7eebcc0cdf1" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant_019d3258-f48a-791b-b255-08814cba75d4" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.peoplesbancorp.com/role/StatementsofChangesinNetAssetsAvailableforBenefits" xlink:type="simple" xlink:href="pebo-20260630.xsd#StatementsofChangesinNetAssetsAvailableforBenefits"/>
  <link:calculationLink xlink:role="http://www.peoplesbancorp.com/role/StatementsofChangesinNetAssetsAvailableforBenefits" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d326f-0536-796e-bd2b-1a7eaad95ee3" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d326f-0536-761a-bf27-662bebb67531" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d326f-0536-796e-bd2b-1a7eaad95ee3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d326f-0536-761a-bf27-662bebb67531" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant_019d326f-0536-7b0e-96f2-6600f6a817cf" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d326f-0536-796e-bd2b-1a7eaad95ee3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant_019d326f-0536-7b0e-96f2-6600f6a817cf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d326f-0536-7667-919e-70079117c962" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d326f-0536-796e-bd2b-1a7eaad95ee3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d326f-0536-7667-919e-70079117c962" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d326e-db77-78cc-b3f8-bc0aa656269a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019d326e-db77-721c-b31c-ddf8defd09c8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d326e-db77-78cc-b3f8-bc0aa656269a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019d326e-db77-721c-b31c-ddf8defd09c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover_019d326e-db77-75cc-b0ef-9e9b23f923de" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d326e-db77-78cc-b3f8-bc0aa656269a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover_019d326e-db77-75cc-b0ef-9e9b23f923de" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionRollover_019d326e-db77-7b0b-873b-4cf56c4fbad9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionRollover"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d326e-db77-78cc-b3f8-bc0aa656269a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionRollover_019d326e-db77-7b0b-873b-4cf56c4fbad9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d326d-5a43-7c3d-99c9-be7832c3994f" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment_019d326d-5a43-710c-a165-50a3753cb4fa" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d326d-5a43-7c3d-99c9-be7832c3994f" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment_019d326d-5a43-710c-a165-50a3753cb4fa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment_019d326d-5a43-796b-8c11-4b72eb74b888" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d326d-5a43-7c3d-99c9-be7832c3994f" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment_019d326d-5a43-796b-8c11-4b72eb74b888" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019d3270-602e-7c38-9693-e06cfa7b00bd" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d3270-602e-7023-a511-fd3954d70480" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019d3270-602e-7c38-9693-e06cfa7b00bd" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d3270-602e-7023-a511-fd3954d70480" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d3270-602e-768d-ad44-333daa8f614b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019d3270-602e-7c38-9693-e06cfa7b00bd" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d3270-602e-768d-ad44-333daa8f614b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d326f-f755-75d3-9c55-9f98b62a2d1d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019d326f-f755-7378-84e5-40954e6613b4" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d326f-f755-75d3-9c55-9f98b62a2d1d" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019d326f-f755-7378-84e5-40954e6613b4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense_019d326f-f755-7d04-b90a-b2921b041929" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d326f-f755-75d3-9c55-9f98b62a2d1d" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense_019d326f-f755-7d04-b90a-b2921b041929" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.peoplesbancorp.com/role/ReconciliationofFinancialStatementstoForm5500ReconciliationofNetAssetsAvailableforBenefitDetails" xlink:type="simple" xlink:href="pebo-20260630.xsd#ReconciliationofFinancialStatementstoForm5500ReconciliationofNetAssetsAvailableforBenefitDetails"/>
  <link:calculationLink xlink:role="http://www.peoplesbancorp.com/role/ReconciliationofFinancialStatementstoForm5500ReconciliationofNetAssetsAvailableforBenefitDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionNetAssetAvailableForBenefit_019d3faa-01e2-7101-a660-3803fc0c639a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionNetAssetAvailableForBenefit"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d3faa-01e2-7ff4-a045-39a4c27fdcb9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionNetAssetAvailableForBenefit_019d3faa-01e2-7101-a660-3803fc0c639a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d3faa-01e2-7ff4-a045-39a4c27fdcb9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanReconciliationToForm5500NetAssetAvailableForBenefitDeemedDistribution_019d3faa-01e2-7477-b5e1-b72feaec2420" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanReconciliationToForm5500NetAssetAvailableForBenefitDeemedDistribution"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionNetAssetAvailableForBenefit_019d3faa-01e2-7101-a660-3803fc0c639a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanReconciliationToForm5500NetAssetAvailableForBenefitDeemedDistribution_019d3faa-01e2-7477-b5e1-b72feaec2420" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_pebo_EBPReconciliationToForm5500NetAssetAvailableForBenefitEmployersContribution_019d3faa-01e2-7ffc-8c2a-08e4021c2183" xlink:href="pebo-20260630.xsd#pebo_EBPReconciliationToForm5500NetAssetAvailableForBenefitEmployersContribution"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionNetAssetAvailableForBenefit_019d3faa-01e2-7101-a660-3803fc0c639a" xlink:to="loc_pebo_EBPReconciliationToForm5500NetAssetAvailableForBenefitEmployersContribution_019d3faa-01e2-7ffc-8c2a-08e4021c2183" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.peoplesbancorp.com/role/ReconciliationofFinancialStatementstoForm5500ReconciliationofEmployersContributionDetails" xlink:type="simple" xlink:href="pebo-20260630.xsd#ReconciliationofFinancialStatementstoForm5500ReconciliationofEmployersContributionDetails"/>
  <link:calculationLink xlink:role="http://www.peoplesbancorp.com/role/ReconciliationofFinancialStatementstoForm5500ReconciliationofEmployersContributionDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionEmployerContribution_019d3faa-21a3-7546-bcf4-44b9c88fe826" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionEmployerContribution"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019d3faa-21a3-74e3-9283-effc58d0fadb" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionEmployerContribution_019d3faa-21a3-7546-bcf4-44b9c88fe826" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019d3faa-21a3-74e3-9283-effc58d0fadb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_pebo_EBPReconciliationToForm5500PriorPeriodIncreaseFromEmployerContribution_019d3faa-21a3-7c5b-ba26-4e95594127c9" xlink:href="pebo-20260630.xsd#pebo_EBPReconciliationToForm5500PriorPeriodIncreaseFromEmployerContribution"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionEmployerContribution_019d3faa-21a3-7546-bcf4-44b9c88fe826" xlink:to="loc_pebo_EBPReconciliationToForm5500PriorPeriodIncreaseFromEmployerContribution_019d3faa-21a3-7c5b-ba26-4e95594127c9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_pebo_EBPReconciliationToForm5500CurrentPeriodDecreaseFromEmployerContribution_019d3faa-21a3-7f41-ac19-055c14c173a7" xlink:href="pebo-20260630.xsd#pebo_EBPReconciliationToForm5500CurrentPeriodDecreaseFromEmployerContribution"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionEmployerContribution_019d3faa-21a3-7546-bcf4-44b9c88fe826" xlink:to="loc_pebo_EBPReconciliationToForm5500CurrentPeriodDecreaseFromEmployerContribution_019d3faa-21a3-7f41-ac19-055c14c173a7" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.peoplesbancorp.com/role/ReconciliationofFinancialStatementstoForm5500ReconciliationofBenefitsPaidtoParticipantsDetails" xlink:type="simple" xlink:href="pebo-20260630.xsd#ReconciliationofFinancialStatementstoForm5500ReconciliationofBenefitsPaidtoParticipantsDetails"/>
  <link:calculationLink xlink:role="http://www.peoplesbancorp.com/role/ReconciliationofFinancialStatementstoForm5500ReconciliationofBenefitsPaidtoParticipantsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionPaymentToParticipant_019d3faa-4c65-769f-9f25-59134ff68fba" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionPaymentToParticipant"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019d3faa-4c65-750d-8186-0cb66da8bf23" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionPaymentToParticipant_019d3faa-4c65-769f-9f25-59134ff68fba" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019d3faa-4c65-750d-8186-0cb66da8bf23" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_pebo_EBPReconciliationToForm5500PaymentToParticipantIncreaseDecreaseFromDistributedParticipantLoan_019d3faa-4c65-7548-a384-40dbd07be1bf" xlink:href="pebo-20260630.xsd#pebo_EBPReconciliationToForm5500PaymentToParticipantIncreaseDecreaseFromDistributedParticipantLoan"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanForm5500CaptionPaymentToParticipant_019d3faa-4c65-769f-9f25-59134ff68fba" xlink:to="loc_pebo_EBPReconciliationToForm5500PaymentToParticipantIncreaseDecreaseFromDistributedParticipantLoan_019d3faa-4c65-7548-a384-40dbd07be1bf" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
