<?xml version="1.0" encoding="us-ascii"?>
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" xlink:type="simple" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/notAll" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#notAll" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/IntangiblesScheduleofIntangibleAssetsDetails" xlink:href="vtix-20260331.xsd#IntangiblesScheduleofIntangibleAssetsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/NotesPayableDetails" xlink:href="vtix-20260331.xsd#NotesPayableDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/PropertyandEquipmentScheduleofPropertyandEquipmentDetails" xlink:href="vtix-20260331.xsd#PropertyandEquipmentScheduleofPropertyandEquipmentDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/CapitalStockDetails" xlink:href="vtix-20260331.xsd#CapitalStockDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/StockCompensationExpenseDetails" xlink:href="vtix-20260331.xsd#StockCompensationExpenseDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/LeasesDetails" xlink:href="vtix-20260331.xsd#LeasesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/CommitmentsandContingenciesDetails" xlink:href="vtix-20260331.xsd#CommitmentsandContingenciesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/InvestmentinJointVentureDetails" xlink:href="vtix-20260331.xsd#InvestmentinJointVentureDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/CapitalStockScheduleofCommonStockforPossibleFutureIssuanceDetails" xlink:href="vtix-20260331.xsd#CapitalStockScheduleofCommonStockforPossibleFutureIssuanceDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/IncomeTaxesDetails" xlink:href="vtix-20260331.xsd#IncomeTaxesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/ConsolidatedBalanceSheetsParentheticals" xlink:href="vtix-20260331.xsd#ConsolidatedBalanceSheetsParentheticals" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/SubsequentEventsDetails" xlink:href="vtix-20260331.xsd#SubsequentEventsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/ConsolidatedBalanceSheets" xlink:href="vtix-20260331.xsd#ConsolidatedBalanceSheets" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/ConsolidatedStatementsofChangesinStockholdersEquityDeficit" xlink:href="vtix-20260331.xsd#ConsolidatedStatementsofChangesinStockholdersEquityDeficit" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/StockCompensationExpenseScheduleofAssumptionsFairValueofOptionGrantsDetails" xlink:href="vtix-20260331.xsd#StockCompensationExpenseScheduleofAssumptionsFairValueofOptionGrantsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetails" xlink:href="vtix-20260331.xsd#SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetails" xlink:href="vtix-20260331.xsd#SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/CapitalStockScheduleofRollforwardofWarrantsDetails" xlink:href="vtix-20260331.xsd#CapitalStockScheduleofRollforwardofWarrantsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:href="https://xbrl.sec.gov/ecd/2026/ecd-2026.xsd#IndividualsOnly" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/Cover" xlink:href="vtix-20260331.xsd#Cover" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/DisaggregationofRevenueScheduleofRevenueStreamsfromPerformanceObligationsDetails" xlink:href="vtix-20260331.xsd#DisaggregationofRevenueScheduleofRevenueStreamsfromPerformanceObligationsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetails" xlink:href="vtix-20260331.xsd#SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/StockCompensationExpenseScheduleofInformationRelatedtoStockOptionsDetails" xlink:href="vtix-20260331.xsd#StockCompensationExpenseScheduleofInformationRelatedtoStockOptionsDetails" xlink:type="simple" />
  <link:roleRef roleURI="http://Virtuix.com/20260331/role/NatureofOperationsDetails" xlink:href="vtix-20260331.xsd#NatureofOperationsDetails" xlink:type="simple" />
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsLineItems" xlink:label="vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsLineItems_1337" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsTable" xlink:label="vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsTable_1327" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractWithCustomerLiabilityCurrent" xlink:label="us-gaap_ContractWithCustomerLiabilityCurrent_1338" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DeferredRevenueArrangementTypeAxis" xlink:label="us-gaap_DeferredRevenueArrangementTypeAxis_1328" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_DeferredRevenueArrangementTypeDomainDomain" xlink:label="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniArenaMember" xlink:label="vtix_OmniArenaMember_1333" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniCareSubscriptionsMember" xlink:label="vtix_OmniCareSubscriptionsMember_1336" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniOneExtendedWarrantyMember" xlink:label="vtix_OmniOneExtendedWarrantyMember_1331" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniOneMember" xlink:label="vtix_OmniOneMember_1330" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniOnlineMember" xlink:label="vtix_OmniOnlineMember_1334" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniProMember" xlink:label="vtix_OmniProMember_1332" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniverseCreditsMember" xlink:label="vtix_OmniverseCreditsMember_1335" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_DeferredRevenueArrangementTypeDomainDomain" xlink:label="vtix_DeferredRevenueArrangementTypeDomainDomain_1329_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsLineItems_1337" xlink:to="vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsTable_1327" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsLineItems_1337" xlink:to="us-gaap_ContractWithCustomerLiabilityCurrent_1338" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofDeferredRevenueDetailsTable_1327" xlink:to="us-gaap_DeferredRevenueArrangementTypeAxis_1328" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DeferredRevenueArrangementTypeAxis_1328" xlink:to="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniArenaMember_1333" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniCareSubscriptionsMember_1336" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniOneExtendedWarrantyMember_1331" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniOneMember_1330" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniOnlineMember_1334" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniProMember_1332" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_DeferredRevenueArrangementTypeDomainDomain_1329" xlink:to="vtix_OmniverseCreditsMember_1335" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DeferredRevenueArrangementTypeAxis_1328" xlink:to="vtix_DeferredRevenueArrangementTypeDomainDomain_1329_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/IntangiblesScheduleofIntangibleAssetsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TrademarksMember" xlink:label="us-gaap_TrademarksMember_1384" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SoftwareAndGameDesignMember" xlink:label="vtix_SoftwareAndGameDesignMember_1383" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_WebsiteMember" xlink:label="vtix_WebsiteMember_1385" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization" xlink:label="us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_1388" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis" xlink:label="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1381" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsGross" xlink:label="us-gaap_FiniteLivedIntangibleAssetsGross_1387" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems" xlink:label="us-gaap_FiniteLivedIntangibleAssetsLineItems_2254" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain" xlink:label="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsNet" xlink:label="us-gaap_FiniteLivedIntangibleAssetsNet_1389" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable" xlink:label="us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_1380" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain" xlink:label="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382_default" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382" xlink:to="us-gaap_TrademarksMember_1384" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382" xlink:to="vtix_SoftwareAndGameDesignMember_1383" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382" xlink:to="vtix_WebsiteMember_1385" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_2254" xlink:to="us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_1388" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_1380" xlink:to="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1381" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_2254" xlink:to="us-gaap_FiniteLivedIntangibleAssetsGross_1387" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1381" xlink:to="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_2254" xlink:to="us-gaap_FiniteLivedIntangibleAssetsNet_1389" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_2254" xlink:to="us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_1380" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1381" xlink:to="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1382_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/NotesPayableDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ScenarioForecastMember" xlink:label="srt_ScenarioForecastMember_2043" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ScenarioUnspecifiedDomain" xlink:label="srt_ScenarioUnspecifiedDomain_2042" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_StatementScenarioAxis" xlink:label="srt_StatementScenarioAxis_2041" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentOfWarrantsGrantedForServices" xlink:label="us-gaap_AdjustmentOfWarrantsGrantedForServices_2000" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_1533" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfDebtDiscountPremium" xlink:label="us-gaap_AmortizationOfDebtDiscountPremium_1536" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfFinancingCosts" xlink:label="us-gaap_AmortizationOfFinancingCosts_1532" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_EconomicInjuryDisasterLoanMember" xlink:label="vtix_EconomicInjuryDisasterLoanMember_1516" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantMember" xlink:label="us-gaap_WarrantMember_1525" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubordinatedDebt" xlink:label="us-gaap_SubordinatedDebt_1538" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_Notes2024Member" xlink:label="vtix_Notes2024Member_1519" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1498" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StreetervilleCapitalLLCMember" xlink:label="vtix_StreetervilleCapitalLLCMember_1499" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1497" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_Notes2024AndSecond2025NotesMember" xlink:label="vtix_Notes2024AndSecond2025NotesMember_1509" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShortTermDebtTypeAxis" xlink:label="us-gaap_ShortTermDebtTypeAxis_1501" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_AggregatePrincipalAmount" xlink:label="vtix_AggregatePrincipalAmount_2267" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2045" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1494" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1495" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConvertibleNotesPayableMember" xlink:label="us-gaap_ConvertibleNotesPayableMember_1503" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAnnualPrincipalPayment" xlink:label="us-gaap_DebtInstrumentAnnualPrincipalPayment_1534" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentConvertibleThresholdPercentageOfStockPriceTrigger" xlink:label="us-gaap_DebtInstrumentConvertibleThresholdPercentageOfStockPriceTrigger_1528" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentFaceAmount" xlink:label="us-gaap_DebtInstrumentFaceAmount_1527" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentIncreaseAccruedInterest" xlink:label="us-gaap_DebtInstrumentIncreaseAccruedInterest_1543" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateEffectivePercentage" xlink:label="us-gaap_DebtInstrumentInterestRateEffectivePercentage_1553" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage" xlink:label="us-gaap_DebtInstrumentInterestRateStatedPercentage_1539" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentIssuanceDate1" xlink:label="us-gaap_DebtInstrumentIssuanceDate1_1555" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentIssuedPrincipal" xlink:label="us-gaap_DebtInstrumentIssuedPrincipal_1544" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentLineItems" xlink:label="us-gaap_DebtInstrumentLineItems_1526" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentMaturityDate" xlink:label="us-gaap_DebtInstrumentMaturityDate_1542" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentMaturityDateRangeEnd1" xlink:label="us-gaap_DebtInstrumentMaturityDateRangeEnd1_1541" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentMaturityDateRangeStart1" xlink:label="us-gaap_DebtInstrumentMaturityDateRangeStart1_1540" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentPeriodicPayment" xlink:label="us-gaap_DebtInstrumentPeriodicPayment_1556" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentRepaidPrincipal" xlink:label="us-gaap_DebtInstrumentRepaidPrincipal_1551" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentUnamortizedDiscount" xlink:label="us-gaap_DebtInstrumentUnamortizedDiscount_1531" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DeferredFinanceCostsNet" xlink:label="us-gaap_DeferredFinanceCostsNet_1535" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1524" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExtinguishmentOfDebtAmount" xlink:label="us-gaap_ExtinguishmentOfDebtAmount_1545" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExtinguishmentOfDebtAxis" xlink:label="us-gaap_ExtinguishmentOfDebtAxis_1517" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExtinguishmentOfDebtTypeDomain" xlink:label="us-gaap_ExtinguishmentOfDebtTypeDomain_1518" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAdjustmentOfWarrants" xlink:label="us-gaap_FairValueAdjustmentOfWarrants_1549" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt" xlink:label="us-gaap_GainsLossesOnExtinguishmentOfDebt_1550" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseDebt" xlink:label="us-gaap_InterestExpenseDebt_1537" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebt" xlink:label="us-gaap_LongTermDebt_1559" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtPercentageBearingFixedInterestRate" xlink:label="us-gaap_LongTermDebtPercentageBearingFixedInterestRate_1530" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermDebtPercentageBearingVariableInterestRate" xlink:label="us-gaap_LongTermDebtPercentageBearingVariableInterestRate_1558" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis" xlink:label="us-gaap_LongtermDebtTypeAxis_1514" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain" xlink:label="us-gaap_LongtermDebtTypeDomain_1515" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsOfDebtExtinguishmentCosts" xlink:label="us-gaap_PaymentsOfDebtExtinguishmentCosts_2126" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_NotesPayableDetailsTable" xlink:label="vtix_NotesPayableDetailsTable_1492" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1493" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_1523" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SeriesBPreferredStockMember" xlink:label="us-gaap_SeriesBPreferredStockMember_1496" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SecuredConvertiblePromissoryNoteMember" xlink:label="vtix_SecuredConvertiblePromissoryNoteMember_1504" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SubordinatedPromissoryNotesMember" xlink:label="vtix_SubordinatedPromissoryNotesMember_1506" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_Second2025NotesMember" xlink:label="vtix_Second2025NotesMember_1508" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodSharesIssuanceOfPreferredStockForDebtExtinguishmentinShares" xlink:label="vtix_StockIssuedDuringPeriodSharesIssuanceOfPreferredStockForDebtExtinguishmentinShares_1547" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharesIssuedPricePerShare" xlink:label="us-gaap_SharesIssuedPricePerShare_2039" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RepaymentsOfOtherDebt" xlink:label="us-gaap_RepaymentsOfOtherDebt_2040" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis" xlink:label="us-gaap_TypeOfArrangementAxis_2044" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_PPPAgreementMember" xlink:label="vtix_PPPAgreementMember_2046" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ExchangeOf2024NotesMember" xlink:label="vtix_ExchangeOf2024NotesMember_2112" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_NumberOfAggregateOfShares" xlink:label="vtix_NumberOfAggregateOfShares_2123" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfDebt" xlink:label="us-gaap_ProceedsFromIssuanceOfDebt_1554" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyMember" xlink:label="us-gaap_RelatedPartyMember_1500" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromOtherDebt" xlink:label="us-gaap_ProceedsFromOtherDebt_1557" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_Notes2025Member" xlink:label="vtix_Notes2025Member_1507" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ExtinguishmentOfAccruedInterest" xlink:label="vtix_ExtinguishmentOfAccruedInterest_1546" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValueIssuanceOfPreferredStockForDebtExtinguishment" xlink:label="vtix_StockIssuedDuringPeriodValueIssuanceOfPreferredStockForDebtExtinguishment_1548" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantsAndRightsOutstanding" xlink:label="us-gaap_WarrantsAndRightsOutstanding_2245" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_WesternTechnologyInvestmentNoteMember" xlink:label="vtix_WesternTechnologyInvestmentNoteMember_1510" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShortTermDebtTypeDomain" xlink:label="us-gaap_ShortTermDebtTypeDomain_1502" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ScenarioUnspecifiedDomain" xlink:label="srt_ScenarioUnspecifiedDomain_2042_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1498_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2045_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1494_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1524_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ExtinguishmentOfDebtTypeDomain" xlink:label="us-gaap_ExtinguishmentOfDebtTypeDomain_1518_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain" xlink:label="us-gaap_LongtermDebtTypeDomain_1515_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShortTermDebtTypeDomain" xlink:label="us-gaap_ShortTermDebtTypeDomain_1502_default" />
    <link:definitionArc xlink:type="arc" order="120.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ScenarioUnspecifiedDomain_2042" xlink:to="srt_ScenarioForecastMember_2043" />
    <link:definitionArc xlink:type="arc" order="119.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_StatementScenarioAxis_2041" xlink:to="srt_ScenarioUnspecifiedDomain_2042" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="srt_StatementScenarioAxis_2041" />
    <link:definitionArc xlink:type="arc" order="83.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_AdjustmentOfWarrantsGrantedForServices_2000" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_1533" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_AmortizationOfDebtDiscountPremium_1536" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_AmortizationOfFinancingCosts_1532" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LongtermDebtTypeDomain_1515" xlink:to="vtix_EconomicInjuryDisasterLoanMember_1516" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1524" xlink:to="us-gaap_WarrantMember_1525" />
    <link:definitionArc xlink:type="arc" order="21.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_SubordinatedDebt_1538" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ExtinguishmentOfDebtTypeDomain_1518" xlink:to="vtix_Notes2024Member_1519" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1497" xlink:to="us-gaap_RelatedPartyDomain_1498" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1498" xlink:to="vtix_StreetervilleCapitalLLCMember_1499" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1497" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_Notes2024AndSecond2025NotesMember_1509" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_ShortTermDebtTypeAxis_1501" />
    <link:definitionArc xlink:type="arc" order="309.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="vtix_AggregatePrincipalAmount_2267" />
    <link:definitionArc xlink:type="arc" order="122.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TypeOfArrangementAxis_2044" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2045" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1493" xlink:to="us-gaap_ClassOfStockDomain_1494" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1494" xlink:to="us-gaap_CommonClassAMember_1495" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="us-gaap_ConvertibleNotesPayableMember_1503" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentAnnualPrincipalPayment_1534" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentConvertibleThresholdPercentageOfStockPriceTrigger_1528" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentFaceAmount_1527" />
    <link:definitionArc xlink:type="arc" order="26.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentIncreaseAccruedInterest_1543" />
    <link:definitionArc xlink:type="arc" order="36.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentInterestRateEffectivePercentage_1553" />
    <link:definitionArc xlink:type="arc" order="22.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentInterestRateStatedPercentage_1539" />
    <link:definitionArc xlink:type="arc" order="38.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentIssuanceDate1_1555" />
    <link:definitionArc xlink:type="arc" order="27.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentIssuedPrincipal_1544" />
    <link:definitionArc xlink:type="arc" order="25.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentMaturityDate_1542" />
    <link:definitionArc xlink:type="arc" order="24.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentMaturityDateRangeEnd1_1541" />
    <link:definitionArc xlink:type="arc" order="23.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentMaturityDateRangeStart1_1540" />
    <link:definitionArc xlink:type="arc" order="39.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentPeriodicPayment_1556" />
    <link:definitionArc xlink:type="arc" order="34.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentRepaidPrincipal_1551" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DebtInstrumentUnamortizedDiscount_1531" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_DeferredFinanceCostsNet_1535" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_1523" xlink:to="us-gaap_EquityComponentDomain_1524" />
    <link:definitionArc xlink:type="arc" order="28.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_ExtinguishmentOfDebtAmount_1545" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_ExtinguishmentOfDebtAxis_1517" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ExtinguishmentOfDebtAxis_1517" xlink:to="us-gaap_ExtinguishmentOfDebtTypeDomain_1518" />
    <link:definitionArc xlink:type="arc" order="32.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_FairValueAdjustmentOfWarrants_1549" />
    <link:definitionArc xlink:type="arc" order="33.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_GainsLossesOnExtinguishmentOfDebt_1550" />
    <link:definitionArc xlink:type="arc" order="20.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_InterestExpenseDebt_1537" />
    <link:definitionArc xlink:type="arc" order="42.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_LongTermDebt_1559" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_LongTermDebtPercentageBearingFixedInterestRate_1530" />
    <link:definitionArc xlink:type="arc" order="41.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_LongTermDebtPercentageBearingVariableInterestRate_1558" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_LongtermDebtTypeAxis_1514" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_LongtermDebtTypeAxis_1514" xlink:to="us-gaap_LongtermDebtTypeDomain_1515" />
    <link:definitionArc xlink:type="arc" order="191.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_PaymentsOfDebtExtinguishmentCosts_2126" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="vtix_NotesPayableDetailsTable_1492" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_StatementClassOfStockAxis_1493" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_StatementEquityComponentsAxis_1523" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1494" xlink:to="us-gaap_SeriesBPreferredStockMember_1496" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_SecuredConvertiblePromissoryNoteMember_1504" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_SubordinatedPromissoryNotesMember_1506" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_Second2025NotesMember_1508" />
    <link:definitionArc xlink:type="arc" order="30.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="vtix_StockIssuedDuringPeriodSharesIssuanceOfPreferredStockForDebtExtinguishmentinShares_1547" />
    <link:definitionArc xlink:type="arc" order="116.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_SharesIssuedPricePerShare_2039" />
    <link:definitionArc xlink:type="arc" order="117.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_RepaymentsOfOtherDebt_2040" />
    <link:definitionArc xlink:type="arc" order="20.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NotesPayableDetailsTable_1492" xlink:to="us-gaap_TypeOfArrangementAxis_2044" />
    <link:definitionArc xlink:type="arc" order="123.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2045" xlink:to="vtix_PPPAgreementMember_2046" />
    <link:definitionArc xlink:type="arc" order="177.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_ExchangeOf2024NotesMember_2112" />
    <link:definitionArc xlink:type="arc" order="188.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="vtix_NumberOfAggregateOfShares_2123" />
    <link:definitionArc xlink:type="arc" order="37.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_ProceedsFromIssuanceOfDebt_1554" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1498" xlink:to="us-gaap_RelatedPartyMember_1500" />
    <link:definitionArc xlink:type="arc" order="40.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_ProceedsFromOtherDebt_1557" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_Notes2025Member_1507" />
    <link:definitionArc xlink:type="arc" order="29.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="vtix_ExtinguishmentOfAccruedInterest_1546" />
    <link:definitionArc xlink:type="arc" order="31.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="vtix_StockIssuedDuringPeriodValueIssuanceOfPreferredStockForDebtExtinguishment_1548" />
    <link:definitionArc xlink:type="arc" order="293.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentLineItems_1526" xlink:to="us-gaap_WarrantsAndRightsOutstanding_2245" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShortTermDebtTypeDomain_1502" xlink:to="vtix_WesternTechnologyInvestmentNoteMember_1510" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ShortTermDebtTypeAxis_1501" xlink:to="us-gaap_ShortTermDebtTypeDomain_1502" />
    <link:definitionArc xlink:type="arc" order="119.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_StatementScenarioAxis_2041" xlink:to="srt_ScenarioUnspecifiedDomain_2042_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1497" xlink:to="us-gaap_RelatedPartyDomain_1498_default" />
    <link:definitionArc xlink:type="arc" order="122.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TypeOfArrangementAxis_2044" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2045_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1493" xlink:to="us-gaap_ClassOfStockDomain_1494_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_1523" xlink:to="us-gaap_EquityComponentDomain_1524_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ExtinguishmentOfDebtAxis_1517" xlink:to="us-gaap_ExtinguishmentOfDebtTypeDomain_1518_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_LongtermDebtTypeAxis_1514" xlink:to="us-gaap_LongtermDebtTypeDomain_1515_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ShortTermDebtTypeAxis_1501" xlink:to="us-gaap_ShortTermDebtTypeDomain_1502_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/PropertyandEquipmentScheduleofPropertyandEquipmentDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:label="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1376" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComputerEquipmentMember" xlink:label="us-gaap_ComputerEquipmentMember_1370" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FurnitureAndFixturesMember" xlink:label="us-gaap_FurnitureAndFixturesMember_1371" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LeaseholdImprovementsMember" xlink:label="us-gaap_LeaseholdImprovementsMember_1373" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MachineryAndEquipmentMember" xlink:label="us-gaap_MachineryAndEquipmentMember_1372" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1368" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentGross" xlink:label="us-gaap_PropertyPlantAndEquipmentGross_1375" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems" xlink:label="us-gaap_PropertyPlantAndEquipmentLineItems_1374" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="us-gaap_PropertyPlantAndEquipmentNet_1377" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable" xlink:label="us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_1367" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1374" xlink:to="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1376" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369" xlink:to="us-gaap_ComputerEquipmentMember_1370" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369" xlink:to="us-gaap_FurnitureAndFixturesMember_1371" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369" xlink:to="us-gaap_LeaseholdImprovementsMember_1373" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369" xlink:to="us-gaap_MachineryAndEquipmentMember_1372" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_1367" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1368" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1374" xlink:to="us-gaap_PropertyPlantAndEquipmentGross_1375" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1374" xlink:to="us-gaap_PropertyPlantAndEquipmentNet_1377" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1368" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1374" xlink:to="us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_1367" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1368" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_1369_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/CapitalStockDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExpirations" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExpirations_1641" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionAxis" xlink:label="us-gaap_RelatedPartyTransactionAxis_1596" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1600" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharePrice" xlink:label="us-gaap_SharePrice_2219" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice_2220" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_2221" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_2222" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1592" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassBMember" xlink:label="us-gaap_CommonClassBMember_1594" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightAxis" xlink:label="us-gaap_ClassOfWarrantOrRightAxis_1616" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts_2144" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_1606" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualAxis" xlink:label="srt_TitleOfIndividualAxis_1613" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ChiefExecutiveOfficerMember" xlink:label="srt_ChiefExecutiveOfficerMember_1615" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_1617" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromStockOptionsExercised" xlink:label="us-gaap_ProceedsFromStockOptionsExercised_2157" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_2159" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_2229" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_2231" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_FinancingInterestExpenses" xlink:label="vtix_FinancingInterestExpenses_2225" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ExchangeAgreementMember" xlink:label="vtix_ExchangeAgreementMember_1605" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_PercentageOfSuccessFee" xlink:label="vtix_PercentageOfSuccessFee_2165" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_RestrictedClassACommonStockMember" xlink:label="vtix_RestrictedClassACommonStockMember_2167" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction" xlink:label="us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_1632" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockCapitalSharesReservedForFutureIssuance" xlink:label="us-gaap_PreferredStockCapitalSharesReservedForFutureIssuance_1629" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConversionOfStockSharesConverted1" xlink:label="us-gaap_ConversionOfStockSharesConverted1_1633" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockValue" xlink:label="us-gaap_CommonStockValue_1635" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_1604" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesAuthorized" xlink:label="us-gaap_CommonStockSharesAuthorized_1620" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockVotingRights" xlink:label="us-gaap_CommonStockVotingRights_1622" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1625" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentOwnedAtCost" xlink:label="us-gaap_InvestmentOwnedAtCost_1627" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodValueNewIssues_2164" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain" xlink:label="srt_TitleOfIndividualWithRelationshipToEntityDomain_1614" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfDebt" xlink:label="us-gaap_ProceedsFromIssuanceOfDebt_2216" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeFairValueOfDerivativeNet" xlink:label="us-gaap_DerivativeFairValueOfDerivativeNet_2217" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_2218" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromFeesReceived" xlink:label="us-gaap_ProceedsFromFeesReceived_2241" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesIssuedForServices" xlink:label="us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_2168" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentIncreaseAccruedInterest" xlink:label="us-gaap_DebtInstrumentIncreaseAccruedInterest_2206" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NotesPayable" xlink:label="us-gaap_NotesPayable_2209" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonMember" xlink:label="us-gaap_TreasuryStockCommonMember_2212" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAxis" xlink:label="us-gaap_DebtInstrumentAxis_2213" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain" xlink:label="us-gaap_DebtInstrumentNameDomain_2214" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1591" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SeriesBPreferredStockMember" xlink:label="us-gaap_SeriesBPreferredStockMember_1595" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionDomain" xlink:label="us-gaap_RelatedPartyTransactionDomain_1597" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1599" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain" xlink:label="us-gaap_PlanNameDomain_1611" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesAuthorized" xlink:label="us-gaap_PreferredStockSharesAuthorized_1621" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SAFENotesMember" xlink:label="vtix_SAFENotesMember_2215" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_CapitalStockDetailsTable" xlink:label="vtix_CapitalStockDetailsTable_1590" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StreetervilleCapitalLLCMember" xlink:label="vtix_StreetervilleCapitalLLCMember_1602" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_AccreditedInvestorsMember" xlink:label="vtix_AccreditedInvestorsMember_1598" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_TwoThousandTwentyFiveOminibusPlanMember" xlink:label="vtix_TwoThousandTwentyFiveOminibusPlanMember_1612" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantMember" xlink:label="us-gaap_WarrantMember_1618" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_DebtAndAccruedInterestExtinguishedForIssuanceOfPreferredStock" xlink:label="vtix_DebtAndAccruedInterestExtinguishedForIssuanceOfPreferredStock_1624" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsCashlessBasisExercised" xlink:label="vtix_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsCashlessBasisExercised_2262" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_CommonStockReservedSharesAuthorized" xlink:label="vtix_CommonStockReservedSharesAuthorized_2269" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1593" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_1609" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_MaximPartnersLLCMember" xlink:label="vtix_MaximPartnersLLCMember_1601" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis" xlink:label="us-gaap_TypeOfArrangementAxis_1603" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_GiftCardConversionAmount" xlink:label="vtix_GiftCardConversionAmount_1637" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_WesternTechnologyInvestmentsMember" xlink:label="vtix_WesternTechnologyInvestmentsMember_2226" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SeriesSeedPreferredStockMember" xlink:label="vtix_SeriesSeedPreferredStockMember_2227" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_CommonStockWarrantsMember" xlink:label="vtix_CommonStockWarrantsMember_2228" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ConvertibleNoteMember" xlink:label="vtix_ConvertibleNoteMember_2235" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_MZHCILLCMember" xlink:label="vtix_MZHCILLCMember_2142" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_FMWMediaWorksLLCMember" xlink:label="vtix_FMWMediaWorksLLCMember_2148" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_DebtWarrantsMember" xlink:label="vtix_DebtWarrantsMember_2249" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockMember" xlink:label="us-gaap_PreferredStockMember_1608" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameAxis" xlink:label="us-gaap_PlanNameAxis_1610" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentsAndCash" xlink:label="us-gaap_InvestmentsAndCash_1626" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockSharesIssuedNetOfSharesForTaxWithholdings" xlink:label="us-gaap_RestrictedStockSharesIssuedNetOfSharesForTaxWithholdings_1628" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_2177" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_2178" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_2179" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_2180" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_StatementScenarioAxis" xlink:label="srt_StatementScenarioAxis_2232" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ScenarioUnspecifiedDomain" xlink:label="srt_ScenarioUnspecifiedDomain_2233" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ScenarioForecastMember" xlink:label="srt_ScenarioForecastMember_2234" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised_1639" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued" xlink:label="us-gaap_CommonStockSharesIssued_1634" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockParOrStatedValuePerShare" xlink:label="us-gaap_CommonStockParOrStatedValuePerShare_2211" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightOutstanding" xlink:label="us-gaap_ClassOfWarrantOrRightOutstanding_2223" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance" xlink:label="us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_1630" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1" xlink:label="us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_1640" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByEachWarrantOrRight" xlink:label="us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByEachWarrantOrRight_1642" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1607" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1600_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1592_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_1617_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_1604_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain" xlink:label="srt_TitleOfIndividualWithRelationshipToEntityDomain_1614_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentNameDomain" xlink:label="us-gaap_DebtInstrumentNameDomain_2214_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionDomain" xlink:label="us-gaap_RelatedPartyTransactionDomain_1597_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain" xlink:label="us-gaap_PlanNameDomain_1611_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_2178_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ScenarioUnspecifiedDomain" xlink:label="srt_ScenarioUnspecifiedDomain_2233_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1607_default" />
    <link:definitionArc xlink:type="arc" order="31.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExpirations_1641" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_RelatedPartyTransactionAxis_1596" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1599" xlink:to="us-gaap_RelatedPartyDomain_1600" />
    <link:definitionArc xlink:type="arc" order="272.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_SharePrice_2219" />
    <link:definitionArc xlink:type="arc" order="273.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice_2220" />
    <link:definitionArc xlink:type="arc" order="274.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_2221" />
    <link:definitionArc xlink:type="arc" order="275.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_2222" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1591" xlink:to="us-gaap_ClassOfStockDomain_1592" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1592" xlink:to="us-gaap_CommonClassBMember_1594" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_ClassOfWarrantOrRightAxis_1616" />
    <link:definitionArc xlink:type="arc" order="203.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts_2144" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_StatementEquityComponentsAxis_1606" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="srt_TitleOfIndividualAxis_1613" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_TitleOfIndividualWithRelationshipToEntityDomain_1614" xlink:to="srt_ChiefExecutiveOfficerMember_1615" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1616" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_1617" />
    <link:definitionArc xlink:type="arc" order="216.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ProceedsFromStockOptionsExercised_2157" />
    <link:definitionArc xlink:type="arc" order="218.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_2159" />
    <link:definitionArc xlink:type="arc" order="279.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_2229" />
    <link:definitionArc xlink:type="arc" order="283.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_2231" />
    <link:definitionArc xlink:type="arc" order="278.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_FinancingInterestExpenses_2225" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember_1604" xlink:to="vtix_ExchangeAgreementMember_1605" />
    <link:definitionArc xlink:type="arc" order="224.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_PercentageOfSuccessFee_2165" />
    <link:definitionArc xlink:type="arc" order="225.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1592" xlink:to="vtix_RestrictedClassACommonStockMember_2167" />
    <link:definitionArc xlink:type="arc" order="22.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_SaleOfStockNumberOfSharesIssuedInTransaction_1632" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_PreferredStockCapitalSharesReservedForFutureIssuance_1629" />
    <link:definitionArc xlink:type="arc" order="23.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ConversionOfStockSharesConverted1_1633" />
    <link:definitionArc xlink:type="arc" order="25.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_CommonStockValue_1635" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TypeOfArrangementAxis_1603" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_1604" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_CommonStockSharesAuthorized_1620" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_CommonStockVotingRights_1622" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1625" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_InvestmentOwnedAtCost_1627" />
    <link:definitionArc xlink:type="arc" order="223.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_StockIssuedDuringPeriodValueNewIssues_2164" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_TitleOfIndividualAxis_1613" xlink:to="srt_TitleOfIndividualWithRelationshipToEntityDomain_1614" />
    <link:definitionArc xlink:type="arc" order="269.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ProceedsFromIssuanceOfDebt_2216" />
    <link:definitionArc xlink:type="arc" order="270.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_DerivativeFairValueOfDerivativeNet_2217" />
    <link:definitionArc xlink:type="arc" order="271.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_2218" />
    <link:definitionArc xlink:type="arc" order="292.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ProceedsFromFeesReceived_2241" />
    <link:definitionArc xlink:type="arc" order="226.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_2168" />
    <link:definitionArc xlink:type="arc" order="260.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_DebtInstrumentIncreaseAccruedInterest_2206" />
    <link:definitionArc xlink:type="arc" order="262.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_NotesPayable_2209" />
    <link:definitionArc xlink:type="arc" order="265.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1607" xlink:to="us-gaap_TreasuryStockCommonMember_2212" />
    <link:definitionArc xlink:type="arc" order="21.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_DebtInstrumentAxis_2213" />
    <link:definitionArc xlink:type="arc" order="267.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DebtInstrumentAxis_2213" xlink:to="us-gaap_DebtInstrumentNameDomain_2214" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_StatementClassOfStockAxis_1591" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1592" xlink:to="us-gaap_SeriesBPreferredStockMember_1595" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionAxis_1596" xlink:to="us-gaap_RelatedPartyTransactionDomain_1597" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1599" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PlanNameAxis_1610" xlink:to="us-gaap_PlanNameDomain_1611" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_PreferredStockSharesAuthorized_1621" />
    <link:definitionArc xlink:type="arc" order="268.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain_2214" xlink:to="vtix_SAFENotesMember_2215" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_CapitalStockDetailsTable_1590" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1600" xlink:to="vtix_StreetervilleCapitalLLCMember_1602" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyTransactionDomain_1597" xlink:to="vtix_AccreditedInvestorsMember_1598" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PlanNameDomain_1611" xlink:to="vtix_TwoThousandTwentyFiveOminibusPlanMember_1612" />
    <link:definitionArc xlink:type="arc" order="267.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1607" xlink:to="us-gaap_WarrantMember_1618" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_DebtAndAccruedInterestExtinguishedForIssuanceOfPreferredStock_1624" />
    <link:definitionArc xlink:type="arc" order="308.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsCashlessBasisExercised_2262" />
    <link:definitionArc xlink:type="arc" order="311.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_CommonStockReservedSharesAuthorized_2269" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1592" xlink:to="us-gaap_CommonClassAMember_1593" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1607" xlink:to="us-gaap_CommonStockMember_1609" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1600" xlink:to="vtix_MaximPartnersLLCMember_1601" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_TypeOfArrangementAxis_1603" />
    <link:definitionArc xlink:type="arc" order="27.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="vtix_GiftCardConversionAmount_1637" />
    <link:definitionArc xlink:type="arc" order="279.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1600" xlink:to="vtix_WesternTechnologyInvestmentsMember_2226" />
    <link:definitionArc xlink:type="arc" order="280.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1592" xlink:to="vtix_SeriesSeedPreferredStockMember_2227" />
    <link:definitionArc xlink:type="arc" order="281.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightDomain_1617" xlink:to="vtix_CommonStockWarrantsMember_2228" />
    <link:definitionArc xlink:type="arc" order="287.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DebtInstrumentNameDomain_2214" xlink:to="vtix_ConvertibleNoteMember_2235" />
    <link:definitionArc xlink:type="arc" order="201.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1600" xlink:to="vtix_MZHCILLCMember_2142" />
    <link:definitionArc xlink:type="arc" order="207.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1600" xlink:to="vtix_FMWMediaWorksLLCMember_2148" />
    <link:definitionArc xlink:type="arc" order="296.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightDomain_1617" xlink:to="vtix_DebtWarrantsMember_2249" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1607" xlink:to="us-gaap_PreferredStockMember_1608" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="us-gaap_PlanNameAxis_1610" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_InvestmentsAndCash_1626" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_RestrictedStockSharesIssuedNetOfSharesForTaxWithholdings_1628" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="srt_RangeAxis_2177" />
    <link:definitionArc xlink:type="arc" order="235.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_2177" xlink:to="srt_RangeMember_2178" />
    <link:definitionArc xlink:type="arc" order="236.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_2178" xlink:to="srt_MaximumMember_2179" />
    <link:definitionArc xlink:type="arc" order="237.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_2178" xlink:to="srt_MinimumMember_2180" />
    <link:definitionArc xlink:type="arc" order="22.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_CapitalStockDetailsTable_1590" xlink:to="srt_StatementScenarioAxis_2232" />
    <link:definitionArc xlink:type="arc" order="285.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_StatementScenarioAxis_2232" xlink:to="srt_ScenarioUnspecifiedDomain_2233" />
    <link:definitionArc xlink:type="arc" order="286.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ScenarioUnspecifiedDomain_2233" xlink:to="srt_ScenarioForecastMember_2234" />
    <link:definitionArc xlink:type="arc" order="29.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised_1639" />
    <link:definitionArc xlink:type="arc" order="24.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_CommonStockSharesIssued_1634" />
    <link:definitionArc xlink:type="arc" order="264.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_CommonStockParOrStatedValuePerShare_2211" />
    <link:definitionArc xlink:type="arc" order="276.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ClassOfWarrantOrRightOutstanding_2223" />
    <link:definitionArc xlink:type="arc" order="20.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_1630" />
    <link:definitionArc xlink:type="arc" order="30.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_1640" />
    <link:definitionArc xlink:type="arc" order="32.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1619" xlink:to="us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByEachWarrantOrRight_1642" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_1606" xlink:to="us-gaap_EquityComponentDomain_1607" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1599" xlink:to="us-gaap_RelatedPartyDomain_1600_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1591" xlink:to="us-gaap_ClassOfStockDomain_1592_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1616" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_1617_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TypeOfArrangementAxis_1603" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_1604_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_TitleOfIndividualAxis_1613" xlink:to="srt_TitleOfIndividualWithRelationshipToEntityDomain_1614_default" />
    <link:definitionArc xlink:type="arc" order="267.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DebtInstrumentAxis_2213" xlink:to="us-gaap_DebtInstrumentNameDomain_2214_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionAxis_1596" xlink:to="us-gaap_RelatedPartyTransactionDomain_1597_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PlanNameAxis_1610" xlink:to="us-gaap_PlanNameDomain_1611_default" />
    <link:definitionArc xlink:type="arc" order="235.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_2177" xlink:to="srt_RangeMember_2178_default" />
    <link:definitionArc xlink:type="arc" order="285.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_StatementScenarioAxis_2232" xlink:to="srt_ScenarioUnspecifiedDomain_2233_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_1606" xlink:to="us-gaap_EquityComponentDomain_1607_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/LeasesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_LeasesDetailsTable" xlink:label="vtix_LeasesDetailsTable_1562" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_1564" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsForRent" xlink:label="us-gaap_PaymentsForRent_1575" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseLiabilityCurrent" xlink:label="us-gaap_OperatingLeaseLiabilityCurrent_1577" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseExpense" xlink:label="us-gaap_OperatingLeaseExpense_1579" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LesseeLeaseDescriptionLineItems" xlink:label="us-gaap_LesseeLeaseDescriptionLineItems_1573" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LeaseExpirationDate1" xlink:label="us-gaap_LeaseExpirationDate1_1574" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseRightOfUseAsset" xlink:label="us-gaap_OperatingLeaseRightOfUseAsset_1576" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent" xlink:label="us-gaap_OperatingLeaseLiabilityNoncurrent_1578" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseWeightedAverageRemainingLeaseTerm1" xlink:label="us-gaap_OperatingLeaseWeightedAverageRemainingLeaseTerm1_1580" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ApartmentSpaceLeasesMember" xlink:label="vtix_ApartmentSpaceLeasesMember_1566" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseWeightedAverageDiscountRatePercent" xlink:label="us-gaap_OperatingLeaseWeightedAverageDiscountRatePercent_1581" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TypeOfArrangementAxis" xlink:label="us-gaap_TypeOfArrangementAxis_1567" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1563" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OfficeEquipmentMember" xlink:label="us-gaap_OfficeEquipmentMember_1565" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2238" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_LeaseAgreementMember" xlink:label="vtix_LeaseAgreementMember_2239" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_1564_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ArrangementsAndNonarrangementTransactionsMember" xlink:label="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2238_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="vtix_LeasesDetailsTable_1562" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1563" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_1564" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_PaymentsForRent_1575" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_OperatingLeaseLiabilityCurrent_1577" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_OperatingLeaseExpense_1579" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_LeaseExpirationDate1_1574" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_OperatingLeaseRightOfUseAsset_1576" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_OperatingLeaseLiabilityNoncurrent_1578" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_OperatingLeaseWeightedAverageRemainingLeaseTerm1_1580" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1564" xlink:to="vtix_ApartmentSpaceLeasesMember_1566" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LesseeLeaseDescriptionLineItems_1573" xlink:to="us-gaap_OperatingLeaseWeightedAverageDiscountRatePercent_1581" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_LeasesDetailsTable_1562" xlink:to="us-gaap_TypeOfArrangementAxis_1567" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_LeasesDetailsTable_1562" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1563" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_1564" xlink:to="us-gaap_OfficeEquipmentMember_1565" />
    <link:definitionArc xlink:type="arc" order="290.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TypeOfArrangementAxis_1567" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2238" />
    <link:definitionArc xlink:type="arc" order="291.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2238" xlink:to="vtix_LeaseAgreementMember_2239" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1563" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_1564_default" />
    <link:definitionArc xlink:type="arc" order="290.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TypeOfArrangementAxis_1567" xlink:to="us-gaap_ArrangementsAndNonarrangementTransactionsMember_2238_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/StockCompensationExpenseDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AllocatedShareBasedCompensationExpense" xlink:label="us-gaap_AllocatedShareBasedCompensationExpense_1660" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_2103" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOptionsAggregateIntrinsicValue" xlink:label="vtix_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOptionsAggregateIntrinsicValue_2127" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_2207" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis" xlink:label="us-gaap_AwardTypeAxis_1656" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_2101" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross" xlink:label="us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_2106" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_2111" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions" xlink:label="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_1665" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsidiarySaleOfStockAxis" xlink:label="us-gaap_SubsidiarySaleOfStockAxis_2116" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ISOsMember" xlink:label="vtix_ISOsMember_2119" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueIssuedForServices" xlink:label="us-gaap_StockIssuedDuringPeriodValueIssuedForServices_2125" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonMember" xlink:label="us-gaap_TreasuryStockCommonMember_2128" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1661" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain" xlink:label="us-gaap_PlanNameDomain_1653" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1657" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesReverseStockSplits" xlink:label="us-gaap_StockIssuedDuringPeriodSharesReverseStockSplits_2097" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised" xlink:label="us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_2099" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_2102" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_2122" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RestrictedStockUnitsRSUMember" xlink:label="us-gaap_RestrictedStockUnitsRSUMember_1658" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_1666" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_2104" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriod_2110" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain_2118" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingNumber_2121" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_2100" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameAxis" xlink:label="us-gaap_PlanNameAxis_1652" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockCompensationExpenseDetailsTable" xlink:label="vtix_StockCompensationExpenseDetailsTable_1651" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_TwoThousandTwentyFiveLongTermIncentivePlanMember" xlink:label="vtix_TwoThousandTwentyFiveLongTermIncentivePlanMember_1654" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_LongTermIncentivePlanMember" xlink:label="vtix_LongTermIncentivePlanMember_2098" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_TwoThousandTwentyFiveOmnibusIncentivePlanMember" xlink:label="vtix_TwoThousandTwentyFiveOmnibusIncentivePlanMember_1655" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_NQSOsMember" xlink:label="vtix_NQSOsMember_2120" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PlanNameDomain" xlink:label="us-gaap_PlanNameDomain_1653_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1657_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_2102_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SaleOfStockNameOfTransactionDomain" xlink:label="us-gaap_SaleOfStockNameOfTransactionDomain_2118_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_AllocatedShareBasedCompensationExpense_1660" />
    <link:definitionArc xlink:type="arc" order="168.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_2102" xlink:to="us-gaap_CommonStockMember_2103" />
    <link:definitionArc xlink:type="arc" order="192.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="vtix_SharebasedCompensationArrangementBySharebasedPaymentAwardEquityInstrumentsOptionsAggregateIntrinsicValue_2127" />
    <link:definitionArc xlink:type="arc" order="261.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_2207" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_StockCompensationExpenseDetailsTable_1651" xlink:to="us-gaap_AwardTypeAxis_1656" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_StockCompensationExpenseDetailsTable_1651" xlink:to="us-gaap_StatementEquityComponentsAxis_2101" />
    <link:definitionArc xlink:type="arc" order="171.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_StockIssuedDuringPeriodSharesRestrictedStockAwardGross_2106" />
    <link:definitionArc xlink:type="arc" order="176.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardSharesIssuedInPeriod_2111" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_EmployeeServiceShareBasedCompensationNonvestedAwardsTotalCompensationCostNotYetRecognizedShareBasedAwardsOtherThanOptions_1665" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_StockCompensationExpenseDetailsTable_1651" xlink:to="us-gaap_SubsidiarySaleOfStockAxis_2116" />
    <link:definitionArc xlink:type="arc" order="184.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain_2118" xlink:to="vtix_ISOsMember_2119" />
    <link:definitionArc xlink:type="arc" order="190.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_StockIssuedDuringPeriodValueIssuedForServices_2125" />
    <link:definitionArc xlink:type="arc" order="169.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_2102" xlink:to="us-gaap_TreasuryStockCommonMember_2128" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_1661" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PlanNameAxis_1652" xlink:to="us-gaap_PlanNameDomain_1653" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AwardTypeAxis_1656" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1657" />
    <link:definitionArc xlink:type="arc" order="163.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_StockIssuedDuringPeriodSharesReverseStockSplits_2097" />
    <link:definitionArc xlink:type="arc" order="164.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_2099" />
    <link:definitionArc xlink:type="arc" order="167.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_2101" xlink:to="us-gaap_EquityComponentDomain_2102" />
    <link:definitionArc xlink:type="arc" order="187.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_2122" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1657" xlink:to="us-gaap_RestrictedStockUnitsRSUMember_1658" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsOutstandingWeightedAverageRemainingContractualTerms_1666" />
    <link:definitionArc xlink:type="arc" order="169.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_2104" />
    <link:definitionArc xlink:type="arc" order="175.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresAndExpirationsInPeriod_2110" />
    <link:definitionArc xlink:type="arc" order="183.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsidiarySaleOfStockAxis_2116" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain_2118" />
    <link:definitionArc xlink:type="arc" order="186.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsVestedAndExpectedToVestOutstandingNumber_2121" />
    <link:definitionArc xlink:type="arc" order="165.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_2100" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_StockCompensationExpenseDetailsTable_1651" xlink:to="us-gaap_PlanNameAxis_1652" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_EmployeeServiceShareBasedCompensationAllocationOfRecognizedPeriodCostsLineItems_1659" xlink:to="vtix_StockCompensationExpenseDetailsTable_1651" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PlanNameDomain_1653" xlink:to="vtix_TwoThousandTwentyFiveLongTermIncentivePlanMember_1654" />
    <link:definitionArc xlink:type="arc" order="164.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PlanNameDomain_1653" xlink:to="vtix_LongTermIncentivePlanMember_2098" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PlanNameDomain_1653" xlink:to="vtix_TwoThousandTwentyFiveOmnibusIncentivePlanMember_1655" />
    <link:definitionArc xlink:type="arc" order="185.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SaleOfStockNameOfTransactionDomain_2118" xlink:to="vtix_NQSOsMember_2120" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PlanNameAxis_1652" xlink:to="us-gaap_PlanNameDomain_1653_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AwardTypeAxis_1656" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_1657_default" />
    <link:definitionArc xlink:type="arc" order="167.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_2101" xlink:to="us-gaap_EquityComponentDomain_2102_default" />
    <link:definitionArc xlink:type="arc" order="183.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsidiarySaleOfStockAxis_2116" xlink:to="us-gaap_SaleOfStockNameOfTransactionDomain_2118_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/CapitalStockScheduleofRollforwardofWarrantsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePrice" xlink:label="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePrice_1408" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_1407" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsGranted" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsGranted_1409" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised_1411" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_2244" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentWeightedAverageExercisePriceGranted" xlink:label="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentWeightedAverageExercisePriceGranted_1410" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightTable" xlink:label="us-gaap_ClassOfWarrantOrRightTable_1403" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantMember" xlink:label="us-gaap_WarrantMember_1405" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePriceExercised" xlink:label="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePriceExercised_1412" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePriceExpired" xlink:label="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePriceExpired_1414" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightAxis" xlink:label="us-gaap_ClassOfWarrantOrRightAxis_1404" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExpirations" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExpirations_1413" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_2244_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePrice_1408" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsOutstandingNumber_1407" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsGranted_1409" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExercised_1411" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1404" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_2244" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentWeightedAverageExercisePriceGranted_1410" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="us-gaap_ClassOfWarrantOrRightTable_1403" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightDomain_2244" xlink:to="us-gaap_WarrantMember_1405" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePriceExercised_1412" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="vtix_ShareBasedCompensationArrangementbyShareBasedPaymentAwardNonOptionEquityInstrumentsWeightedAverageExercisePriceExpired_1414" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ClassOfWarrantOrRightTable_1403" xlink:to="us-gaap_ClassOfWarrantOrRightAxis_1404" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_1406" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardNonOptionEquityInstrumentsExpirations_1413" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1404" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_2244_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/CommitmentsandContingenciesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RoyaltyExpense" xlink:label="us-gaap_RoyaltyExpense_2130" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_RoyaltyExpensePerUnit" xlink:label="vtix_RoyaltyExpensePerUnit_2131" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingenciesTable" xlink:label="us-gaap_LossContingenciesTable_2132" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_2133" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_2134" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_2135" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_2136" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LossContingenciesLineItems" xlink:label="us-gaap_LossContingenciesLineItems_2248" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_2134_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LossContingenciesLineItems_2248" xlink:to="us-gaap_RoyaltyExpense_2130" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LossContingenciesLineItems_2248" xlink:to="vtix_RoyaltyExpensePerUnit_2131" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_LossContingenciesLineItems_2248" xlink:to="us-gaap_LossContingenciesTable_2132" />
    <link:definitionArc xlink:type="arc" order="194.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_LossContingenciesTable_2132" xlink:to="srt_RangeAxis_2133" />
    <link:definitionArc xlink:type="arc" order="195.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_2133" xlink:to="srt_RangeMember_2134" />
    <link:definitionArc xlink:type="arc" order="196.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_2134" xlink:to="srt_MinimumMember_2135" />
    <link:definitionArc xlink:type="arc" order="197.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_2134" xlink:to="srt_MaximumMember_2136" />
    <link:definitionArc xlink:type="arc" order="195.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_2133" xlink:to="srt_RangeMember_2134_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/SubsequentEventsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantsAndRightsOutstandingMaturityDate" xlink:label="us-gaap_WarrantsAndRightsOutstandingMaturityDate_2160" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_1736" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightAxis" xlink:label="us-gaap_ClassOfWarrantOrRightAxis_1735" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1711" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued" xlink:label="us-gaap_CommonStockSharesIssued_1752" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtDefaultLongtermDebtAmount" xlink:label="us-gaap_DebtDefaultLongtermDebtAmount_2149" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_AggregateShares" xlink:label="vtix_AggregateShares_2268" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ConvertiblePromissoryNotesMember" xlink:label="vtix_ConvertiblePromissoryNotesMember_2156" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_EquityFinancingWarrant1Member" xlink:label="vtix_EquityFinancingWarrant1Member_1738" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_EquityFinancingWarrant2Member" xlink:label="vtix_EquityFinancingWarrant2Member_1739" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights" xlink:label="us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_1766" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1712" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_FMWMediaWorksLLCMember" xlink:label="vtix_FMWMediaWorksLLCMember_1722" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StreetervilleCapitalLLCMember" xlink:label="vtix_StreetervilleCapitalLLCMember_1723" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SubsequentEventsDetailsTable" xlink:label="vtix_SubsequentEventsDetailsTable_1709" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1" xlink:label="us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_1758" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DebtInstrumentAnnualPrincipalPayment" xlink:label="us-gaap_DebtInstrumentAnnualPrincipalPayment_2146" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1753" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventLineItems" xlink:label="us-gaap_SubsequentEventLineItems_1751" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventMember" xlink:label="us-gaap_SubsequentEventMember_1719" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeAxis" xlink:label="us-gaap_SubsequentEventTypeAxis_1717" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeDomain" xlink:label="us-gaap_SubsequentEventTypeDomain_1718" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_WarrantExercisePriceDecrease" xlink:label="us-gaap_WarrantExercisePriceDecrease_2158" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAdjustmentOfWarrants" xlink:label="us-gaap_FairValueAdjustmentOfWarrants_2161" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeAxis" xlink:label="us-gaap_LongtermDebtTypeAxis_2151" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain" xlink:label="us-gaap_LongtermDebtTypeDomain_2152" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfWarrants" xlink:label="us-gaap_ProceedsFromIssuanceOfWarrants_1767" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1721" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1720" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1710" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfWarrantOrRightDomain" xlink:label="us-gaap_ClassOfWarrantOrRightDomain_1736_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1711_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SubsequentEventTypeDomain" xlink:label="us-gaap_SubsequentEventTypeDomain_1718_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongtermDebtTypeDomain" xlink:label="us-gaap_LongtermDebtTypeDomain_2152_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1721_default" />
    <link:definitionArc xlink:type="arc" order="219.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_WarrantsAndRightsOutstandingMaturityDate_2160" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1735" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_1736" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SubsequentEventsDetailsTable_1709" xlink:to="us-gaap_ClassOfWarrantOrRightAxis_1735" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1710" xlink:to="us-gaap_ClassOfStockDomain_1711" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_CommonStockSharesIssued_1752" />
    <link:definitionArc xlink:type="arc" order="208.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_DebtDefaultLongtermDebtAmount_2149" />
    <link:definitionArc xlink:type="arc" order="310.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="vtix_AggregateShares_2268" />
    <link:definitionArc xlink:type="arc" order="215.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LongtermDebtTypeDomain_2152" xlink:to="vtix_ConvertiblePromissoryNotesMember_2156" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightDomain_1736" xlink:to="vtix_EquityFinancingWarrant1Member_1738" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfWarrantOrRightDomain_1736" xlink:to="vtix_EquityFinancingWarrant2Member_1739" />
    <link:definitionArc xlink:type="arc" order="24.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_ClassOfWarrantOrRightNumberOfSecuritiesCalledByWarrantsOrRights_1766" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1711" xlink:to="us-gaap_CommonClassAMember_1712" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1721" xlink:to="vtix_FMWMediaWorksLLCMember_1722" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1721" xlink:to="vtix_StreetervilleCapitalLLCMember_1723" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="vtix_SubsequentEventsDetailsTable_1709" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_ClassOfWarrantOrRightExercisePriceOfWarrantsOrRights1_1758" />
    <link:definitionArc xlink:type="arc" order="205.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_DebtInstrumentAnnualPrincipalPayment_2146" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1753" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventTypeDomain_1718" xlink:to="us-gaap_SubsequentEventMember_1719" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SubsequentEventsDetailsTable_1709" xlink:to="us-gaap_SubsequentEventTypeAxis_1717" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_SubsequentEventTypeAxis_1717" xlink:to="us-gaap_SubsequentEventTypeDomain_1718" />
    <link:definitionArc xlink:type="arc" order="217.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_WarrantExercisePriceDecrease_2158" />
    <link:definitionArc xlink:type="arc" order="220.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_FairValueAdjustmentOfWarrants_2161" />
    <link:definitionArc xlink:type="arc" order="21.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SubsequentEventsDetailsTable_1709" xlink:to="us-gaap_LongtermDebtTypeAxis_2151" />
    <link:definitionArc xlink:type="arc" order="211.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_LongtermDebtTypeAxis_2151" xlink:to="us-gaap_LongtermDebtTypeDomain_2152" />
    <link:definitionArc xlink:type="arc" order="25.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_SubsequentEventLineItems_1751" xlink:to="us-gaap_ProceedsFromIssuanceOfWarrants_1767" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1720" xlink:to="us-gaap_RelatedPartyDomain_1721" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SubsequentEventsDetailsTable_1709" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1720" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SubsequentEventsDetailsTable_1709" xlink:to="us-gaap_StatementClassOfStockAxis_1710" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_ClassOfWarrantOrRightAxis_1735" xlink:to="us-gaap_ClassOfWarrantOrRightDomain_1736_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1710" xlink:to="us-gaap_ClassOfStockDomain_1711_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_SubsequentEventTypeAxis_1717" xlink:to="us-gaap_SubsequentEventTypeDomain_1718_default" />
    <link:definitionArc xlink:type="arc" order="211.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_LongtermDebtTypeAxis_2151" xlink:to="us-gaap_LongtermDebtTypeDomain_2152_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1720" xlink:to="us-gaap_RelatedPartyDomain_1721_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/ConsolidatedBalanceSheets" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsCurrentAbstract" xlink:label="us-gaap_AssetsCurrentAbstract_1012" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsAndNotesReceivableNet" xlink:label="us-gaap_AccountsAndNotesReceivableNet_1014" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent" xlink:label="us-gaap_PrepaidExpenseAndOtherAssetsCurrent_1016" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsNoncurrentAbstract" xlink:label="us-gaap_AssetsNoncurrentAbstract_1018" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment" xlink:label="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1020" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsGross" xlink:label="us-gaap_FiniteLivedIntangibleAssetsGross_1022" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsNet" xlink:label="us-gaap_FiniteLivedIntangibleAssetsNet_1024" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="us-gaap_LiabilitiesAndStockholdersEquity_1051" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Liabilities" xlink:label="us-gaap_Liabilities_1044" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockValue" xlink:label="us-gaap_PreferredStockValue_1046" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsAccumulatedDeficit" xlink:label="us-gaap_RetainedEarningsAccumulatedDeficit_1049" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems" xlink:label="us-gaap_StatementLineItems_1011" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent" xlink:label="us-gaap_OperatingLeaseLiabilityNoncurrent_1042" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_GiftCardLiability" xlink:label="vtix_GiftCardLiability_1034" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsCurrent" xlink:label="us-gaap_AssetsCurrent_1017" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Assets" xlink:label="us-gaap_Assets_1029" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesNoncurrentAbstract" xlink:label="us-gaap_LiabilitiesNoncurrentAbstract_1040" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseLiabilityCurrent" xlink:label="us-gaap_OperatingLeaseLiabilityCurrent_1038" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NotesPayableCurrent" xlink:label="us-gaap_NotesPayableCurrent_1036" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccruedLiabilitiesCurrent" xlink:label="us-gaap_AccruedLiabilitiesCurrent_1032" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesCurrentAbstract" xlink:label="us-gaap_LiabilitiesCurrentAbstract_1030" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsNoncurrent" xlink:label="us-gaap_AssetsNoncurrent_1028" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherAssetsNoncurrent" xlink:label="us-gaap_OtherAssetsNoncurrent_1026" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InventoryNet" xlink:label="us-gaap_InventoryNet_1015" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="us-gaap_CashAndCashEquivalentsAtCarryingValue_1013" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassBMember" xlink:label="us-gaap_CommonClassBMember_1010" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentGross" xlink:label="us-gaap_PropertyPlantAndEquipmentGross_1019" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="us-gaap_PropertyPlantAndEquipmentNet_1021" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyMember" xlink:label="us-gaap_RelatedPartyMember_1006" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapital" xlink:label="us-gaap_AdditionalPaidInCapital_1048" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1008" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1005" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1009" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermNotesPayable" xlink:label="us-gaap_LongTermNotesPayable_1940" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization" xlink:label="us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_1023" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures" xlink:label="us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_1025" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLeaseRightOfUseAsset" xlink:label="us-gaap_OperatingLeaseRightOfUseAsset_1027" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsPayableCurrent" xlink:label="us-gaap_AccountsPayableCurrent_1031" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ContractWithCustomerLiabilityCurrent" xlink:label="us-gaap_ContractWithCustomerLiabilityCurrent_1033" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherLiabilitiesCurrent" xlink:label="us-gaap_OtherLiabilitiesCurrent_1035" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherLoansPayableCurrent" xlink:label="us-gaap_OtherLoansPayableCurrent_1037" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesCurrent" xlink:label="us-gaap_LiabilitiesCurrent_1039" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquityAbstract" xlink:label="us-gaap_StockholdersEquityAbstract_1045" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable" xlink:label="us-gaap_StatementTable_1003" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1007" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity" xlink:label="us-gaap_StockholdersEquity_1050" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LongTermLoansPayable" xlink:label="us-gaap_LongTermLoansPayable_1041" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesNoncurrent" xlink:label="us-gaap_LiabilitiesNoncurrent_1043" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockValue" xlink:label="us-gaap_CommonStockValue_1047" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1004" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1008_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1005_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_AssetsCurrentAbstract_1012" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract_1012" xlink:to="us-gaap_AccountsAndNotesReceivableNet_1014" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract_1012" xlink:to="us-gaap_PrepaidExpenseAndOtherAssetsCurrent_1016" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_AssetsNoncurrentAbstract_1018" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentGross_1019" xlink:to="us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_1020" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_FiniteLivedIntangibleAssetsGross_1022" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_FiniteLivedIntangibleAssetsNet_1024" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StockholdersEquityAbstract_1045" xlink:to="us-gaap_LiabilitiesAndStockholdersEquity_1051" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract_1040" xlink:to="us-gaap_Liabilities_1044" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StockholdersEquityAbstract_1045" xlink:to="us-gaap_PreferredStockValue_1046" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StockholdersEquityAbstract_1045" xlink:to="us-gaap_RetainedEarningsAccumulatedDeficit_1049" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract_1040" xlink:to="us-gaap_OperatingLeaseLiabilityNoncurrent_1042" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="vtix_GiftCardLiability_1034" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PrepaidExpenseAndOtherAssetsCurrent_1016" xlink:to="us-gaap_AssetsCurrent_1017" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_Assets_1029" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_LiabilitiesNoncurrentAbstract_1040" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_OperatingLeaseLiabilityCurrent_1038" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_NotesPayableCurrent_1036" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_AccruedLiabilitiesCurrent_1032" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_LiabilitiesCurrentAbstract_1030" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingLeaseRightOfUseAsset_1027" xlink:to="us-gaap_AssetsNoncurrent_1028" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_OtherAssetsNoncurrent_1026" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract_1012" xlink:to="us-gaap_InventoryNet_1015" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsCurrentAbstract_1012" xlink:to="us-gaap_CashAndCashEquivalentsAtCarryingValue_1013" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1008" xlink:to="us-gaap_CommonClassBMember_1010" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_PropertyPlantAndEquipmentGross_1019" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_PropertyPlantAndEquipmentNet_1021" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1005" xlink:to="us-gaap_RelatedPartyMember_1006" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StockholdersEquityAbstract_1045" xlink:to="us-gaap_AdditionalPaidInCapital_1048" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1007" xlink:to="us-gaap_ClassOfStockDomain_1008" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1004" xlink:to="us-gaap_RelatedPartyDomain_1005" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1008" xlink:to="us-gaap_CommonClassAMember_1009" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract_1040" xlink:to="us-gaap_LongTermNotesPayable_1940" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsGross_1022" xlink:to="us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_1023" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_InvestmentsInAffiliatesSubsidiariesAssociatesAndJointVentures_1025" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_AssetsNoncurrentAbstract_1018" xlink:to="us-gaap_OperatingLeaseRightOfUseAsset_1027" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_AccountsPayableCurrent_1031" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_ContractWithCustomerLiabilityCurrent_1033" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_OtherLiabilitiesCurrent_1035" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesCurrentAbstract_1030" xlink:to="us-gaap_OtherLoansPayableCurrent_1037" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingLeaseLiabilityCurrent_1038" xlink:to="us-gaap_LiabilitiesCurrent_1039" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_StockholdersEquityAbstract_1045" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems_1011" xlink:to="us-gaap_StatementTable_1003" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_1003" xlink:to="us-gaap_StatementClassOfStockAxis_1007" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RetainedEarningsAccumulatedDeficit_1049" xlink:to="us-gaap_StockholdersEquity_1050" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_LiabilitiesNoncurrentAbstract_1040" xlink:to="us-gaap_LongTermLoansPayable_1041" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_OperatingLeaseLiabilityNoncurrent_1042" xlink:to="us-gaap_LiabilitiesNoncurrent_1043" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StockholdersEquityAbstract_1045" xlink:to="us-gaap_CommonStockValue_1047" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_1003" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1004" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1007" xlink:to="us-gaap_ClassOfStockDomain_1008_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1004" xlink:to="us-gaap_RelatedPartyDomain_1005_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/InvestmentinJointVentureDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ShareOfGainInJointVenture" xlink:label="vtix_ShareOfGainInJointVenture_1701" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_VirtuixManufacturingLimitedMember" xlink:label="vtix_VirtuixManufacturingLimitedMember_1688" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingExpenses" xlink:label="us-gaap_OperatingExpenses_1696" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss" xlink:label="us-gaap_NetIncomeLoss_1697" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners" xlink:label="us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners_1693" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_JointVentureFormationFairValueOfJointVenture" xlink:label="us-gaap_JointVentureFormationFairValueOfJointVenture_2035" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_OwnershipAxis" xlink:label="srt_OwnershipAxis_1686" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_OwnershipDomain" xlink:label="srt_OwnershipDomain_1687" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsPayableOtherCurrentAndNoncurrent" xlink:label="us-gaap_AccountsPayableOtherCurrentAndNoncurrent_2072" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CorporateJointVentureMember" xlink:label="us-gaap_CorporateJointVentureMember_1691" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostOfRevenue" xlink:label="us-gaap_CostOfRevenue_1695" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncomeLossFromEquityMethodInvestments" xlink:label="us-gaap_IncomeLossFromEquityMethodInvestments_2025" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OperatingRevenue" xlink:label="vtix_OperatingRevenue_1694" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_InvestmentinJointVentureDetailsTable" xlink:label="vtix_InvestmentinJointVentureDetailsTable_1685" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfEquityMethodInvestmentsLineItems" xlink:label="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsDeficitEliminated" xlink:label="us-gaap_RetainedEarningsDeficitEliminated_2037" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionsByRelatedPartyAxis" xlink:label="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1689" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyTransactionAmountsOfTransaction" xlink:label="us-gaap_RelatedPartyTransactionAmountsOfTransaction_2071" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1690" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OtherLiabilities" xlink:label="us-gaap_OtherLiabilities_2036" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_OwnershipDomain" xlink:label="srt_OwnershipDomain_1687_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RelatedPartyDomain" xlink:label="us-gaap_RelatedPartyDomain_1690_default" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="vtix_ShareOfGainInJointVenture_1701" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_OwnershipDomain_1687" xlink:to="vtix_VirtuixManufacturingLimitedMember_1688" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_OperatingExpenses_1696" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_NetIncomeLoss_1697" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners_1693" />
    <link:definitionArc xlink:type="arc" order="112.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_JointVentureFormationFairValueOfJointVenture_2035" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_InvestmentinJointVentureDetailsTable_1685" xlink:to="srt_OwnershipAxis_1686" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_OwnershipAxis_1686" xlink:to="srt_OwnershipDomain_1687" />
    <link:definitionArc xlink:type="arc" order="143.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_AccountsPayableOtherCurrentAndNoncurrent_2072" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_RelatedPartyDomain_1690" xlink:to="us-gaap_CorporateJointVentureMember_1691" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_CostOfRevenue_1695" />
    <link:definitionArc xlink:type="arc" order="102.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_IncomeLossFromEquityMethodInvestments_2025" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="vtix_OperatingRevenue_1694" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="vtix_InvestmentinJointVentureDetailsTable_1685" />
    <link:definitionArc xlink:type="arc" order="114.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_RetainedEarningsDeficitEliminated_2037" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_InvestmentinJointVentureDetailsTable_1685" xlink:to="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1689" />
    <link:definitionArc xlink:type="arc" order="142.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_RelatedPartyTransactionAmountsOfTransaction_2071" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1689" xlink:to="us-gaap_RelatedPartyDomain_1690" />
    <link:definitionArc xlink:type="arc" order="113.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ScheduleOfEquityMethodInvestmentsLineItems_1692" xlink:to="us-gaap_OtherLiabilities_2036" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_OwnershipAxis_1686" xlink:to="srt_OwnershipDomain_1687_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_RelatedPartyTransactionsByRelatedPartyAxis_1689" xlink:to="us-gaap_RelatedPartyDomain_1690_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/DisaggregationofRevenueScheduleofRevenueStreamsfromPerformanceObligationsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductOrServiceAxis" xlink:label="srt_ProductOrServiceAxis_1430" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain" xlink:label="srt_ProductsAndServicesDomain_1431" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DisaggregationOfRevenueLineItems" xlink:label="us-gaap_DisaggregationOfRevenueLineItems_1437" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DisaggregationOfRevenueTable" xlink:label="us-gaap_DisaggregationOfRevenueTable_1429" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Revenues" xlink:label="us-gaap_Revenues_1438" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ResaleActivityMember" xlink:label="vtix_ResaleActivityMember_2075" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniverseCreditsMember" xlink:label="vtix_OmniverseCreditsMember_1433" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniProUnitsAndAccessoriesMember" xlink:label="vtix_OmniProUnitsAndAccessoriesMember_1432" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniOneMember" xlink:label="vtix_OmniOneMember_2074" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniCareProgramMember" xlink:label="vtix_OmniCareProgramMember_1434" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_OmniArenaMember" xlink:label="vtix_OmniArenaMember_1435" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ProductsAndServicesDomain" xlink:label="srt_ProductsAndServicesDomain_1431_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_DisaggregationOfRevenueTable_1429" xlink:to="srt_ProductOrServiceAxis_1430" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_ProductOrServiceAxis_1430" xlink:to="srt_ProductsAndServicesDomain_1431" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_DisaggregationOfRevenueLineItems_1437" xlink:to="us-gaap_DisaggregationOfRevenueTable_1429" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DisaggregationOfRevenueLineItems_1437" xlink:to="us-gaap_Revenues_1438" />
    <link:definitionArc xlink:type="arc" order="145.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ProductsAndServicesDomain_1431" xlink:to="vtix_ResaleActivityMember_2075" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ProductsAndServicesDomain_1431" xlink:to="vtix_OmniverseCreditsMember_1433" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ProductsAndServicesDomain_1431" xlink:to="vtix_OmniProUnitsAndAccessoriesMember_1432" />
    <link:definitionArc xlink:type="arc" order="144.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ProductsAndServicesDomain_1431" xlink:to="vtix_OmniOneMember_2074" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ProductsAndServicesDomain_1431" xlink:to="vtix_OmniCareProgramMember_1434" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_ProductsAndServicesDomain_1431" xlink:to="vtix_OmniArenaMember_1435" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_ProductOrServiceAxis_1430" xlink:to="srt_ProductsAndServicesDomain_1431_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/CapitalStockScheduleofCommonStockforPossibleFutureIssuanceDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ClassACommonLTIP2014Member" xlink:label="vtix_ClassACommonLTIP2014Member_2273" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ClassACommonLTIP2025Member" xlink:label="vtix_ClassACommonLTIP2025Member_2274" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ClassACommonOmnibus2025Member" xlink:label="vtix_ClassACommonOmnibus2025Member_2275" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ClassBCommonOutstandingMember" xlink:label="vtix_ClassBCommonOutstandingMember_2276" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable" xlink:label="us-gaap_StatementTable_2270" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_2271" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_2272" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_CommonStockReservedSharesAuthorized" xlink:label="vtix_CommonStockReservedSharesAuthorized_2281" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_IrrevocableTransferAgenetReservePPAMember" xlink:label="vtix_IrrevocableTransferAgenetReservePPAMember_2277" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_RegisteredSharesUnderlyingCVPPPPMember" xlink:label="vtix_RegisteredSharesUnderlyingCVPPPPMember_2278" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_RegisteredSharesUnderlyingCVPWarrantsMember" xlink:label="vtix_RegisteredSharesUnderlyingCVPWarrantsMember_2279" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ScheduleOfCommonStockForPossibleFutureIssuanceLineItems" xlink:label="vtix_ScheduleOfCommonStockForPossibleFutureIssuanceLineItems_2280" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_2272_default" />
    <link:definitionArc xlink:type="arc" order="315.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_ClassACommonLTIP2014Member_2273" />
    <link:definitionArc xlink:type="arc" order="316.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_ClassACommonLTIP2025Member_2274" />
    <link:definitionArc xlink:type="arc" order="317.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_ClassACommonOmnibus2025Member_2275" />
    <link:definitionArc xlink:type="arc" order="318.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_ClassBCommonOutstandingMember_2276" />
    <link:definitionArc xlink:type="arc" order="312.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="vtix_ScheduleOfCommonStockForPossibleFutureIssuanceLineItems_2280" xlink:to="us-gaap_StatementTable_2270" />
    <link:definitionArc xlink:type="arc" order="313.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_2270" xlink:to="us-gaap_StatementClassOfStockAxis_2271" />
    <link:definitionArc xlink:type="arc" order="314.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_2271" xlink:to="us-gaap_ClassOfStockDomain_2272" />
    <link:definitionArc xlink:type="arc" order="323.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="vtix_ScheduleOfCommonStockForPossibleFutureIssuanceLineItems_2280" xlink:to="vtix_CommonStockReservedSharesAuthorized_2281" />
    <link:definitionArc xlink:type="arc" order="319.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_IrrevocableTransferAgenetReservePPAMember_2277" />
    <link:definitionArc xlink:type="arc" order="320.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_RegisteredSharesUnderlyingCVPPPPMember_2278" />
    <link:definitionArc xlink:type="arc" order="321.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_2272" xlink:to="vtix_RegisteredSharesUnderlyingCVPWarrantsMember_2279" />
    <link:definitionArc xlink:type="arc" order="314.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_2271" xlink:to="us-gaap_ClassOfStockDomain_2272_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/IncomeTaxesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TaxYear2017Member" xlink:label="us-gaap_TaxYear2017Member_1672" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TaxPeriodAxis" xlink:label="us-gaap_TaxPeriodAxis_1670" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationLineItems" xlink:label="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingLossCarryforwards" xlink:label="us-gaap_OperatingLossCarryforwards_1680" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StandardTaxRate" xlink:label="vtix_StandardTaxRate_2024" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance" xlink:label="us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance_2015" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_EnterpriseIncomeTaxExpense" xlink:label="vtix_EnterpriseIncomeTaxExpense_2019" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_1674" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_1676" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1675" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_1677" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate" xlink:label="us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate_1679" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ExpirationDate" xlink:label="vtix_ExpirationDate_1681" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationOtherReconcilingItemsPercent" xlink:label="us-gaap_EffectiveIncomeTaxRateReconciliationOtherReconcilingItemsPercent_1682" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TaxYear2018Member" xlink:label="us-gaap_TaxYear2018Member_2012" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TaxPeriodDomain" xlink:label="us-gaap_TaxPeriodDomain_1671" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_IncomeTaxesDetailsTable" xlink:label="vtix_IncomeTaxesDetailsTable_1669" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_PercentageOfTaxLimitation" xlink:label="vtix_PercentageOfTaxLimitation_2023" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1675_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TaxPeriodDomain" xlink:label="us-gaap_TaxPeriodDomain_1671_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_TaxPeriodDomain_1671" xlink:to="us-gaap_TaxYear2017Member_1672" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_IncomeTaxesDetailsTable_1669" xlink:to="us-gaap_TaxPeriodAxis_1670" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="us-gaap_OperatingLossCarryforwards_1680" />
    <link:definitionArc xlink:type="arc" order="101.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="vtix_StandardTaxRate_2024" />
    <link:definitionArc xlink:type="arc" order="92.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance_2015" />
    <link:definitionArc xlink:type="arc" order="96.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="vtix_EnterpriseIncomeTaxExpense_2019" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_IncomeTaxesDetailsTable_1669" xlink:to="srt_RangeAxis_1674" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1675" xlink:to="srt_MinimumMember_1676" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_1674" xlink:to="srt_RangeMember_1675" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1675" xlink:to="srt_MaximumMember_1677" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate_1679" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="vtix_ExpirationDate_1681" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="us-gaap_EffectiveIncomeTaxRateReconciliationOtherReconcilingItemsPercent_1682" />
    <link:definitionArc xlink:type="arc" order="89.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_TaxPeriodDomain_1671" xlink:to="us-gaap_TaxYear2018Member_2012" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_TaxPeriodAxis_1670" xlink:to="us-gaap_TaxPeriodDomain_1671" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="vtix_IncomeTaxesDetailsTable_1669" />
    <link:definitionArc xlink:type="arc" order="100.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EffectiveIncomeTaxRateReconciliationLineItems_1678" xlink:to="vtix_PercentageOfTaxLimitation_2023" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_1674" xlink:to="srt_RangeMember_1675_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_TaxPeriodAxis_1670" xlink:to="us-gaap_TaxPeriodDomain_1671_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/ConsolidatedBalanceSheetsParentheticals" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesIssued" xlink:label="us-gaap_CommonStockSharesIssued_1067" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockParOrStatedValuePerShare" xlink:label="us-gaap_CommonStockParOrStatedValuePerShare_1065" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesOutstanding" xlink:label="us-gaap_PreferredStockSharesOutstanding_1063" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesAuthorized" xlink:label="us-gaap_PreferredStockSharesAuthorized_1061" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems" xlink:label="us-gaap_StatementLineItems_1059" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1057" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1055" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassBMember" xlink:label="us-gaap_CommonClassBMember_1058" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockParOrStatedValuePerShare" xlink:label="us-gaap_PreferredStockParOrStatedValuePerShare_1060" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockSharesIssued" xlink:label="us-gaap_PreferredStockSharesIssued_1062" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable" xlink:label="us-gaap_StatementTable_1054" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1056" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockLiquidationPreferenceValue" xlink:label="us-gaap_PreferredStockLiquidationPreferenceValue_1064" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesAuthorized" xlink:label="us-gaap_CommonStockSharesAuthorized_1066" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockSharesOutstanding" xlink:label="us-gaap_CommonStockSharesOutstanding_1068" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1056_default" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_CommonStockSharesIssued_1067" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_CommonStockParOrStatedValuePerShare_1065" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_PreferredStockSharesOutstanding_1063" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_PreferredStockSharesAuthorized_1061" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1056" xlink:to="us-gaap_CommonClassAMember_1057" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_1054" xlink:to="us-gaap_StatementClassOfStockAxis_1055" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1056" xlink:to="us-gaap_CommonClassBMember_1058" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_PreferredStockParOrStatedValuePerShare_1060" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_PreferredStockSharesIssued_1062" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_StatementTable_1054" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1055" xlink:to="us-gaap_ClassOfStockDomain_1056" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_PreferredStockLiquidationPreferenceValue_1064" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_CommonStockSharesAuthorized_1066" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1059" xlink:to="us-gaap_CommonStockSharesOutstanding_1068" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1055" xlink:to="us-gaap_ClassOfStockDomain_1056_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/Cover" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentsIncorporatedByReferenceTextBlock" xlink:label="dei_DocumentsIncorporatedByReferenceTextBlock_1932" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentTransitionReport" xlink:label="dei_DocumentTransitionReport_1782" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentType" xlink:label="dei_DocumentType_1779" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_SecurityExchangeName" xlink:label="dei_SecurityExchangeName_1796" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_Security12bTitle" xlink:label="dei_Security12bTitle_1794" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressAddressLine1" xlink:label="dei_EntityAddressAddressLine1_1787" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressAddressLine2" xlink:label="dei_EntityAddressAddressLine2_1788" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressCityOrTown" xlink:label="dei_EntityAddressCityOrTown_1789" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityWellKnownSeasonedIssuer" xlink:label="dei_EntityWellKnownSeasonedIssuer_1930" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityVoluntaryFilers" xlink:label="dei_EntityVoluntaryFilers_1931" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_IcfrAuditorAttestationFlag" xlink:label="dei_IcfrAuditorAttestationFlag_1934" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1773" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1775" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_AmendmentFlag" xlink:label="dei_AmendmentFlag_1805" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_CityAreaCode" xlink:label="dei_CityAreaCode_1792" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_CurrentFiscalYearEndDate" xlink:label="dei_CurrentFiscalYearEndDate_1808" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentAnnualReport" xlink:label="dei_DocumentAnnualReport_1929" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressPostalZipCode" xlink:label="dei_EntityAddressPostalZipCode_1791" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityAddressStateOrProvince" xlink:label="dei_EntityAddressStateOrProvince_1790" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityCentralIndexKey" xlink:label="dei_EntityCentralIndexKey_1778" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityCommonStockSharesOutstanding" xlink:label="dei_EntityCommonStockSharesOutstanding_1804" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityCurrentReportingStatus" xlink:label="dei_EntityCurrentReportingStatus_1797" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityEmergingGrowthCompany" xlink:label="dei_EntityEmergingGrowthCompany_1801" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityExTransitionPeriod" xlink:label="dei_EntityExTransitionPeriod_1802" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityFileNumber" xlink:label="dei_EntityFileNumber_1783" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityFilerCategory" xlink:label="dei_EntityFilerCategory_1799" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityIncorporationStateCountryCode" xlink:label="dei_EntityIncorporationStateCountryCode_1785" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityInteractiveDataCurrent" xlink:label="dei_EntityInteractiveDataCurrent_1798" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityPublicFloat" xlink:label="dei_EntityPublicFloat_1933" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityRegistrantName" xlink:label="dei_EntityRegistrantName_1784" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityShellCompany" xlink:label="dei_EntityShellCompany_1803" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntitySmallBusiness" xlink:label="dei_EntitySmallBusiness_1800" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_EntityTaxIdentificationNumber" xlink:label="dei_EntityTaxIdentificationNumber_1786" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1774" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassBMember" xlink:label="us-gaap_CommonClassBMember_1776" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_LocalPhoneNumber" xlink:label="dei_LocalPhoneNumber_1793" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_TradingSymbol" xlink:label="dei_TradingSymbol_1795" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentFinStmtErrorCorrectionFlag" xlink:label="dei_DocumentFinStmtErrorCorrectionFlag_1935" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentFiscalPeriodFocus" xlink:label="dei_DocumentFiscalPeriodFocus_1807" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentFiscalYearFocus" xlink:label="dei_DocumentFiscalYearFocus_1806" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentInformationLineItems" xlink:label="dei_DocumentInformationLineItems_1777" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentInformationTable" xlink:label="dei_DocumentInformationTable_1772" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/dei/2026/dei-2026.xsd#dei_DocumentPeriodEndDate" xlink:label="dei_DocumentPeriodEndDate_1781" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1774_default" />
    <link:definitionArc xlink:type="arc" order="22.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentsIncorporatedByReferenceTextBlock_1932" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentTransitionReport_1782" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentType_1779" />
    <link:definitionArc xlink:type="arc" order="36.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_SecurityExchangeName_1796" />
    <link:definitionArc xlink:type="arc" order="34.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_Security12bTitle_1794" />
    <link:definitionArc xlink:type="arc" order="23.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityAddressAddressLine1_1787" />
    <link:definitionArc xlink:type="arc" order="24.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityAddressAddressLine2_1788" />
    <link:definitionArc xlink:type="arc" order="25.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityAddressCityOrTown_1789" />
    <link:definitionArc xlink:type="arc" order="38.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityWellKnownSeasonedIssuer_1930" />
    <link:definitionArc xlink:type="arc" order="39.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityVoluntaryFilers_1931" />
    <link:definitionArc xlink:type="arc" order="20.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_IcfrAuditorAttestationFlag_1934" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="dei_DocumentInformationTable_1772" xlink:to="us-gaap_StatementClassOfStockAxis_1773" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1774" xlink:to="us-gaap_CommonClassAMember_1775" />
    <link:definitionArc xlink:type="arc" order="21.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_AmendmentFlag_1805" />
    <link:definitionArc xlink:type="arc" order="31.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_CityAreaCode_1792" />
    <link:definitionArc xlink:type="arc" order="37.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_CurrentFiscalYearEndDate_1808" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentAnnualReport_1929" />
    <link:definitionArc xlink:type="arc" order="27.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityAddressPostalZipCode_1791" />
    <link:definitionArc xlink:type="arc" order="26.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityAddressStateOrProvince_1790" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityCentralIndexKey_1778" />
    <link:definitionArc xlink:type="arc" order="47.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityCommonStockSharesOutstanding_1804" />
    <link:definitionArc xlink:type="arc" order="40.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityCurrentReportingStatus_1797" />
    <link:definitionArc xlink:type="arc" order="43.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityEmergingGrowthCompany_1801" />
    <link:definitionArc xlink:type="arc" order="44.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityExTransitionPeriod_1802" />
    <link:definitionArc xlink:type="arc" order="29.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityFileNumber_1783" />
    <link:definitionArc xlink:type="arc" order="41.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityFilerCategory_1799" />
    <link:definitionArc xlink:type="arc" order="33.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityIncorporationStateCountryCode_1785" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityInteractiveDataCurrent_1798" />
    <link:definitionArc xlink:type="arc" order="46.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityPublicFloat_1933" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityRegistrantName_1784" />
    <link:definitionArc xlink:type="arc" order="45.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityShellCompany_1803" />
    <link:definitionArc xlink:type="arc" order="42.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntitySmallBusiness_1800" />
    <link:definitionArc xlink:type="arc" order="30.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_EntityTaxIdentificationNumber_1786" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1773" xlink:to="us-gaap_ClassOfStockDomain_1774" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1774" xlink:to="us-gaap_CommonClassBMember_1776" />
    <link:definitionArc xlink:type="arc" order="32.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_LocalPhoneNumber_1793" />
    <link:definitionArc xlink:type="arc" order="35.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_TradingSymbol_1795" />
    <link:definitionArc xlink:type="arc" order="28.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentFinStmtErrorCorrectionFlag_1935" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentFiscalPeriodFocus_1807" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentFiscalYearFocus_1806" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentInformationTable_1772" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="dei_DocumentInformationLineItems_1777" xlink:to="dei_DocumentPeriodEndDate_1781" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1773" xlink:to="us-gaap_ClassOfStockDomain_1774_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/ConsolidatedStatementsofChangesinStockholdersEquityDeficit" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodValueNewIssues_1120" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValuePreferredStockReclass" xlink:label="vtix_StockIssuedDuringPeriodValuePreferredStockReclass_1122" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_AdjustmentsToAdditionalPaidInCapitalInvestorIncentive" xlink:label="vtix_AdjustmentsToAdditionalPaidInCapitalInvestorIncentive_1124" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueIssuedForServices" xlink:label="us-gaap_StockIssuedDuringPeriodValueIssuedForServices_1129" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonShares" xlink:label="us-gaap_TreasuryStockCommonShares_1116" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_1103" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodSharesFractionalShareRounding" xlink:label="vtix_StockIssuedDuringPeriodSharesFractionalShareRounding_1134" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementTable" xlink:label="us-gaap_StatementTable_1100" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockMember" xlink:label="us-gaap_PreferredStockMember_1102" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfUnits" xlink:label="us-gaap_StockIssuedDuringPeriodValueConversionOfUnits_1131" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValueFractionalShareRounding" xlink:label="vtix_StockIssuedDuringPeriodValueFractionalShareRounding_1133" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockRepurchasedDuringPeriodValue" xlink:label="us-gaap_StockRepurchasedDuringPeriodValue_1135" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValueIssuanceOfPreferredStockForDebtExtinguishment" xlink:label="vtix_StockIssuedDuringPeriodValueIssuanceOfPreferredStockForDebtExtinguishment_1126" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_AdjustmentsToAdditionalPaidInCapitalIssuanceOfWarrantsForDebtExtinguishment" xlink:label="vtix_AdjustmentsToAdditionalPaidInCapitalIssuanceOfWarrantsForDebtExtinguishment_1128" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1112" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationStockOptionsRequisiteServicePeriodRecognition" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationStockOptionsRequisiteServicePeriodRecognition_1117" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockRepurchasedDuringPeriodShares" xlink:label="us-gaap_StockRepurchasedDuringPeriodShares_1136" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValueConversionOfSAFENotes" xlink:label="vtix_StockIssuedDuringPeriodValueConversionOfSAFENotes_1138" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss" xlink:label="us-gaap_NetIncomeLoss_1140" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharesOutstanding" xlink:label="us-gaap_SharesOutstanding_1142" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodSharesIssuanceOfPreferredStockForDebtExtinguishmentinShares" xlink:label="vtix_StockIssuedDuringPeriodSharesIssuanceOfPreferredStockForDebtExtinguishmentinShares_1127" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsMember" xlink:label="us-gaap_RetainedEarningsMember_1107" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassBMember" xlink:label="us-gaap_CommonClassBMember_1111" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementClassOfStockAxis" xlink:label="us-gaap_StatementClassOfStockAxis_1109" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValueExerciseOfCommonStockWarrants" xlink:label="vtix_StockIssuedDuringPeriodValueExerciseOfCommonStockWarrants_1118" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementLineItems" xlink:label="us-gaap_StatementLineItems_1113" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts_1943" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_AdjustmentsToAdditionalPaidInCapitalWarrantModification" xlink:label="vtix_AdjustmentsToAdditionalPaidInCapitalWarrantModification_1944" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodValueExerciseOfEmployeeStockOptions" xlink:label="vtix_StockIssuedDuringPeriodValueExerciseOfEmployeeStockOptions_1945" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodSharesExerciseOfEmployeeStockOptions" xlink:label="vtix_StockIssuedDuringPeriodSharesExerciseOfEmployeeStockOptions_1946" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesIssuedForServices" xlink:label="us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_1130" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfUnits" xlink:label="us-gaap_StockIssuedDuringPeriodSharesConversionOfUnits_1132" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapitalMember" xlink:label="us-gaap_AdditionalPaidInCapitalMember_1105" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised" xlink:label="us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_1119" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues" xlink:label="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1121" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodSharesPreferredStockReclassinShares" xlink:label="vtix_StockIssuedDuringPeriodSharesPreferredStockReclassinShares_1123" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued" xlink:label="us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_1125" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_1101" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity" xlink:label="us-gaap_StockholdersEquity_1114" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_IssuanceOfSAFENotes" xlink:label="vtix_IssuanceOfSAFENotes_2266" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TreasuryStockCommonMember" xlink:label="us-gaap_TreasuryStockCommonMember_1104" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1108" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ConversionOfPromissoryNotes" xlink:label="vtix_ConversionOfPromissoryNotes_1971" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ConversionOfPromissoryNotesShares" xlink:label="vtix_ConversionOfPromissoryNotesShares_1972" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockIssuedDuringPeriodSharesConversionOfSAFENotesinShares" xlink:label="vtix_StockIssuedDuringPeriodSharesConversionOfSAFENotesinShares_1139" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SAFENotesMember" xlink:label="vtix_SAFENotesMember_2265" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonClassAMember" xlink:label="us-gaap_CommonClassAMember_1110" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ClassOfStockDomain" xlink:label="us-gaap_ClassOfStockDomain_1112_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_1108_default" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodValueNewIssues_1120" />
    <link:definitionArc xlink:type="arc" order="33.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodValuePreferredStockReclass_1122" />
    <link:definitionArc xlink:type="arc" order="35.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_AdjustmentsToAdditionalPaidInCapitalInvestorIncentive_1124" />
    <link:definitionArc xlink:type="arc" order="22.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodValueIssuedForServices_1129" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_TreasuryStockCommonShares_1116" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1108" xlink:to="us-gaap_CommonStockMember_1103" />
    <link:definitionArc xlink:type="arc" order="37.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodSharesFractionalShareRounding_1134" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StatementTable_1100" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1108" xlink:to="us-gaap_PreferredStockMember_1102" />
    <link:definitionArc xlink:type="arc" order="38.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodValueConversionOfUnits_1131" />
    <link:definitionArc xlink:type="arc" order="36.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodValueFractionalShareRounding_1133" />
    <link:definitionArc xlink:type="arc" order="20.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockRepurchasedDuringPeriodValue_1135" />
    <link:definitionArc xlink:type="arc" order="28.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodValueIssuanceOfPreferredStockForDebtExtinguishment_1126" />
    <link:definitionArc xlink:type="arc" order="30.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_AdjustmentsToAdditionalPaidInCapitalIssuanceOfWarrantsForDebtExtinguishment_1128" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementClassOfStockAxis_1109" xlink:to="us-gaap_ClassOfStockDomain_1112" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalShareBasedCompensationStockOptionsRequisiteServicePeriodRecognition_1117" />
    <link:definitionArc xlink:type="arc" order="21.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockRepurchasedDuringPeriodShares_1136" />
    <link:definitionArc xlink:type="arc" order="25.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodValueConversionOfSAFENotes_1138" />
    <link:definitionArc xlink:type="arc" order="27.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_NetIncomeLoss_1140" />
    <link:definitionArc xlink:type="arc" order="45.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_SharesOutstanding_1142" />
    <link:definitionArc xlink:type="arc" order="29.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodSharesIssuanceOfPreferredStockForDebtExtinguishmentinShares_1127" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1108" xlink:to="us-gaap_RetainedEarningsMember_1107" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1112" xlink:to="us-gaap_CommonClassBMember_1111" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_1100" xlink:to="us-gaap_StatementClassOfStockAxis_1109" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodValueExerciseOfCommonStockWarrants_1118" />
    <link:definitionArc xlink:type="arc" order="42.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalStockIssuedIssuanceCosts_1943" />
    <link:definitionArc xlink:type="arc" order="43.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_AdjustmentsToAdditionalPaidInCapitalWarrantModification_1944" />
    <link:definitionArc xlink:type="arc" order="40.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodValueExerciseOfEmployeeStockOptions_1945" />
    <link:definitionArc xlink:type="arc" order="41.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodSharesExerciseOfEmployeeStockOptions_1946" />
    <link:definitionArc xlink:type="arc" order="23.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodSharesIssuedForServices_1130" />
    <link:definitionArc xlink:type="arc" order="39.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodSharesConversionOfUnits_1132" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1108" xlink:to="us-gaap_AdditionalPaidInCapitalMember_1105" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_1119" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockIssuedDuringPeriodSharesNewIssues_1121" />
    <link:definitionArc xlink:type="arc" order="34.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodSharesPreferredStockReclassinShares_1123" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_AdjustmentsToAdditionalPaidInCapitalWarrantIssued_1125" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_StatementTable_1100" xlink:to="us-gaap_StatementEquityComponentsAxis_1101" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="us-gaap_StockholdersEquity_1114" />
    <link:definitionArc xlink:type="arc" order="24.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_IssuanceOfSAFENotes_2266" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1108" xlink:to="us-gaap_TreasuryStockCommonMember_1104" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_1101" xlink:to="us-gaap_EquityComponentDomain_1108" />
    <link:definitionArc xlink:type="arc" order="31.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_ConversionOfPromissoryNotes_1971" />
    <link:definitionArc xlink:type="arc" order="32.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_ConversionOfPromissoryNotesShares_1972" />
    <link:definitionArc xlink:type="arc" order="26.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_StatementLineItems_1113" xlink:to="vtix_StockIssuedDuringPeriodSharesConversionOfSAFENotesinShares_1139" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_1108" xlink:to="vtix_SAFENotesMember_2265" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ClassOfStockDomain_1112" xlink:to="us-gaap_CommonClassAMember_1110" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementClassOfStockAxis_1109" xlink:to="us-gaap_ClassOfStockDomain_1112_default" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_1101" xlink:to="us-gaap_EquityComponentDomain_1108_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/StockCompensationExpenseScheduleofInformationRelatedtoStockOptionsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_2084" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_2085" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_2093" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice_2094" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_2086" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_2087" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockOptionMember" xlink:label="us-gaap_StockOptionMember_2260" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_2259" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeInstrumentRiskAxis" xlink:label="us-gaap_DerivativeInstrumentRiskAxis_2258" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable" xlink:label="us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_2257" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_2091" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_2090" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_2089" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_2088" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_2081" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised" xlink:label="us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_2082" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_2083" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_2096" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeContractTypeDomain" xlink:label="us-gaap_DerivativeContractTypeDomain_2259_default" />
    <link:definitionArc xlink:type="arc" order="18.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExpirationsInPeriod_2084" />
    <link:definitionArc xlink:type="arc" order="20.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingNumber_2085" />
    <link:definitionArc xlink:type="arc" order="17.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsForfeituresInPeriodWeightedAverageExercisePrice_2093" />
    <link:definitionArc xlink:type="arc" order="19.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExpirationsInPeriodWeightedAverageExercisePrice_2094" />
    <link:definitionArc xlink:type="arc" order="22.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableNumber_2086" />
    <link:definitionArc xlink:type="arc" order="24.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardOptionsOutstandingWeightedAverageRemainingContractualTerm2_2087" />
    <link:definitionArc xlink:type="arc" order="306.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_DerivativeContractTypeDomain_2259" xlink:to="us-gaap_StockOptionMember_2260" />
    <link:definitionArc xlink:type="arc" order="305.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_2258" xlink:to="us-gaap_DerivativeContractTypeDomain_2259" />
    <link:definitionArc xlink:type="arc" order="304.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_2257" xlink:to="us-gaap_DerivativeInstrumentRiskAxis_2258" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ScheduleOfShareBasedCompensationArrangementsByShareBasedPaymentAwardTable_2257" />
    <link:definitionArc xlink:type="arc" order="15.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsExercisesInPeriodWeightedAverageExercisePrice_2091" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_2090" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsOutstandingWeightedAverageExercisePrice_2089" />
    <link:definitionArc xlink:type="arc" order="25.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_2088" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsGrantsInPeriodGross_2081" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_2082" />
    <link:definitionArc xlink:type="arc" order="16.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsForfeituresInPeriod_2083" />
    <link:definitionArc xlink:type="arc" order="23.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2261" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardOptionsExercisableWeightedAverageExercisePrice_2096" />
    <link:definitionArc xlink:type="arc" order="305.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_DerivativeInstrumentRiskAxis_2258" xlink:to="us-gaap_DerivativeContractTypeDomain_2259_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/StockCompensationExpenseScheduleofAssumptionsFairValueofOptionGrantsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_NQSOsMember" xlink:label="vtix_NQSOsMember_2030" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_StockCompensationExpenseScheduleOfAssumptionsFairValueOfOptionGrantsTable" xlink:label="vtix_StockCompensationExpenseScheduleOfAssumptionsFairValueOfOptionGrantsTable_2014" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_ISOsMember" xlink:label="vtix_ISOsMember_2029" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice_2017" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_2018" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_2020" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2028" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AwardTypeAxis" xlink:label="us-gaap_AwardTypeAxis_2027" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1" xlink:label="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_2022" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate" xlink:label="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_2021" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain" xlink:label="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2028_default" />
    <link:definitionArc xlink:type="arc" order="107.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2028" xlink:to="vtix_NQSOsMember_2030" />
    <link:definitionArc xlink:type="arc" order="91.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" xlink:to="vtix_StockCompensationExpenseScheduleOfAssumptionsFairValueOfOptionGrantsTable_2014" />
    <link:definitionArc xlink:type="arc" order="106.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2028" xlink:to="vtix_ISOsMember_2029" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExercisePrice_2017" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedDividendRate_2018" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsRiskFreeInterestRate_2020" />
    <link:definitionArc xlink:type="arc" order="105.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_AwardTypeAxis_2027" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2028" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_StockCompensationExpenseScheduleOfAssumptionsFairValueOfOptionGrantsTable_2014" xlink:to="us-gaap_AwardTypeAxis_2027" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" xlink:to="us-gaap_SharebasedCompensationArrangementBySharebasedPaymentAwardFairValueAssumptionsExpectedTerm1_2022" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardLineItems_2255" xlink:to="us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardFairValueAssumptionsExpectedVolatilityRate_2021" />
    <link:definitionArc xlink:type="arc" order="105.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_AwardTypeAxis_2027" xlink:to="us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_2028_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/NatureofOperationsDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions" xlink:label="us-gaap_StockIssuedDuringPeriodSharesAcquisitions_2047" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_2049" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockMember" xlink:label="us-gaap_CommonStockMember_2050" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_OwnershipDomain" xlink:label="srt_OwnershipDomain_2240" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_OwnershipAxis" xlink:label="srt_OwnershipAxis_1456" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MinorityInterestLineItems" xlink:label="us-gaap_MinorityInterestLineItems_1459" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_VirtuixManufacturingLimitedMember" xlink:label="vtix_VirtuixManufacturingLimitedMember_1458" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_NatureofOperationsDetailsTable" xlink:label="vtix_NatureofOperationsDetailsTable_1455" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_VirtuixArabiaLLCMember" xlink:label="vtix_VirtuixArabiaLLCMember_2250" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners" xlink:label="us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners_1461" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MinorityInterestOwnershipPercentageByParent" xlink:label="us-gaap_MinorityInterestOwnershipPercentageByParent_1460" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StatementEquityComponentsAxis" xlink:label="us-gaap_StatementEquityComponentsAxis_2048" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_EquityComponentDomain" xlink:label="us-gaap_EquityComponentDomain_2049_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_OwnershipDomain" xlink:label="srt_OwnershipDomain_2240_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_MinorityInterestLineItems_1459" xlink:to="us-gaap_StockIssuedDuringPeriodSharesAcquisitions_2047" />
    <link:definitionArc xlink:type="arc" order="126.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_StatementEquityComponentsAxis_2048" xlink:to="us-gaap_EquityComponentDomain_2049" />
    <link:definitionArc xlink:type="arc" order="127.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_EquityComponentDomain_2049" xlink:to="us-gaap_CommonStockMember_2050" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_OwnershipAxis_1456" xlink:to="srt_OwnershipDomain_2240" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NatureofOperationsDetailsTable_1455" xlink:to="srt_OwnershipAxis_1456" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_OwnershipDomain_2240" xlink:to="vtix_VirtuixManufacturingLimitedMember_1458" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_MinorityInterestLineItems_1459" xlink:to="vtix_NatureofOperationsDetailsTable_1455" />
    <link:definitionArc xlink:type="arc" order="297.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_OwnershipDomain_2240" xlink:to="vtix_VirtuixArabiaLLCMember_2250" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_MinorityInterestLineItems_1459" xlink:to="us-gaap_MinorityInterestOwnershipPercentageByNoncontrollingOwners_1461" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_MinorityInterestLineItems_1459" xlink:to="us-gaap_MinorityInterestOwnershipPercentageByParent_1460" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_NatureofOperationsDetailsTable_1455" xlink:to="us-gaap_StatementEquityComponentsAxis_2048" />
    <link:definitionArc xlink:type="arc" order="126.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_StatementEquityComponentsAxis_2048" xlink:to="us-gaap_EquityComponentDomain_2049_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_OwnershipAxis_1456" xlink:to="srt_OwnershipDomain_2240_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_1322" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_1321" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_1319" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1320" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ComputerEquipmentMember" xlink:label="us-gaap_ComputerEquipmentMember_1314" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FurnitureAndFixturesMember" xlink:label="us-gaap_FurnitureAndFixturesMember_1315" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LeaseholdImprovementsMember" xlink:label="us-gaap_LeaseholdImprovementsMember_1318" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MachineryAndEquipmentMember" xlink:label="us-gaap_MachineryAndEquipmentMember_1316" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OfficeEquipmentMember" xlink:label="us-gaap_OfficeEquipmentMember_1317" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis" xlink:label="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1313" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentLineItems" xlink:label="us-gaap_PropertyPlantAndEquipmentLineItems_1323" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife" xlink:label="us-gaap_PropertyPlantAndEquipmentUsefulLife_1324" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetailsTable" xlink:label="vtix_SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetailsTable_1312" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1320_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain" xlink:label="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208_default" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1320" xlink:to="srt_MaximumMember_1322" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1320" xlink:to="srt_MinimumMember_1321" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetailsTable_1312" xlink:to="srt_RangeAxis_1319" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_1319" xlink:to="srt_RangeMember_1320" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" xlink:to="us-gaap_ComputerEquipmentMember_1314" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" xlink:to="us-gaap_FurnitureAndFixturesMember_1315" />
    <link:definitionArc xlink:type="arc" order="14.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" xlink:to="us-gaap_LeaseholdImprovementsMember_1318" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" xlink:to="us-gaap_MachineryAndEquipmentMember_1316" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" xlink:to="us-gaap_OfficeEquipmentMember_1317" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetailsTable_1312" xlink:to="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1313" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1313" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1323" xlink:to="us-gaap_PropertyPlantAndEquipmentUsefulLife_1324" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_PropertyPlantAndEquipmentLineItems_1323" xlink:to="vtix_SummaryofSignificantAccountingPoliciesScheduleofEstimatedUsefulLivesDetailsTable_1312" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_1319" xlink:to="srt_RangeMember_1320_default" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_PropertyPlantAndEquipmentByTypeAxis_1313" xlink:to="us-gaap_PropertyPlantAndEquipmentTypeDomain_2208_default" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_JanGoetgelukMember" xlink:label="vtix_JanGoetgelukMember_2283" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_DavidAllanMember" xlink:label="vtix_DavidAllanMember_2284" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/ecd/2026/ecd-2026.xsd#ecd_AllIndividualsMember" xlink:label="ecd_AllIndividualsMember_34680" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="ecd_AllIndividualsMember_34680" xlink:to="vtix_JanGoetgelukMember_2283" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="ecd_AllIndividualsMember_34680" xlink:to="vtix_DavidAllanMember_2284" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://Virtuix.com/20260331/role/SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetails" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_WebsiteMember" xlink:label="vtix_WebsiteMember_1999" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetailsTable" xlink:label="vtix_SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetailsTable_1441" />
    <link:loc xlink:type="locator" xlink:href="vtix-20260331.xsd#vtix_FiniteLivedIntangibleAssetUsefulLifeDescription" xlink:label="vtix_FiniteLivedIntangibleAssetUsefulLifeDescription_1452" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_TrademarksMember" xlink:label="us-gaap_TrademarksMember_1445" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_SoftwareDevelopmentMember" xlink:label="us-gaap_SoftwareDevelopmentMember_1444" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife" xlink:label="us-gaap_FiniteLivedIntangibleAssetUsefulLife_1451" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MinimumMember" xlink:label="srt_MinimumMember_1998" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeAxis" xlink:label="srt_RangeAxis_1995" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1996" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CustomerListsMember" xlink:label="us-gaap_CustomerListsMember_1446" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis" xlink:label="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1442" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems" xlink:label="us-gaap_FiniteLivedIntangibleAssetsLineItems_1450" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain" xlink:label="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_MaximumMember" xlink:label="srt_MaximumMember_1997" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_RangeMember" xlink:label="srt_RangeMember_1996_default" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain" xlink:label="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443_default" />
    <link:definitionArc xlink:type="arc" order="82.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443" xlink:to="vtix_WebsiteMember_1999" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="true" use="optional" xbrldt:contextElement="segment" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_1450" xlink:to="vtix_SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetailsTable_1441" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_1450" xlink:to="vtix_FiniteLivedIntangibleAssetUsefulLifeDescription_1452" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443" xlink:to="us-gaap_TrademarksMember_1445" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443" xlink:to="us-gaap_SoftwareDevelopmentMember_1444" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsLineItems_1450" xlink:to="us-gaap_FiniteLivedIntangibleAssetUsefulLife_1451" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1996" xlink:to="srt_MinimumMember_1998" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetailsTable_1441" xlink:to="srt_RangeAxis_1995" />
    <link:definitionArc xlink:type="arc" order="81.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="srt_RangeAxis_1995" xlink:to="srt_RangeMember_1996" />
    <link:definitionArc xlink:type="arc" order="13.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443" xlink:to="us-gaap_CustomerListsMember_1446" />
    <link:definitionArc xlink:type="arc" order="11.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="vtix_SummaryofSignificantAccountingPoliciesScheduleofIntangibleAssetsEstimatedUsefulLivesDetailsTable_1441" xlink:to="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1442" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1442" xlink:to="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443" />
    <link:definitionArc xlink:type="arc" order="12.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_RangeMember_1996" xlink:to="srt_MaximumMember_1997" />
    <link:definitionArc xlink:type="arc" order="81.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="srt_RangeAxis_1995" xlink:to="srt_RangeMember_1996_default" />
    <link:definitionArc xlink:type="arc" order="10.00" priority="1" xbrldt:closed="false" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_1442" xlink:to="us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_1443_default" />
  </link:definitionLink>
  <link:roleRef roleURI="http://fasb.org/srt/role/srt-eedm/ExtensibleEnumerationLists" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-roles-2026.xsd#eedm1" xlink:type="simple" />
  <link:definitionLink xlink:role="http://fasb.org/srt/role/srt-eedm/ExtensibleEnumerationLists" xlink:type="extended" xlink:title="definitionLink">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_TitleOfIndividualWithRelationshipToEntityDomain" xlink:label="srt_TitleOfIndividualWithRelationshipToEntityDomain" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/srt/2026/elts/srt-2026.xsd#srt_ChiefExecutiveOfficerMember" xlink:label="us-gaap_ChiefExecutiveOfficerMember" />
    <link:definitionArc xlink:type="arc" order="0" priority="1" xbrldt:closed="true" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="srt_TitleOfIndividualWithRelationshipToEntityDomain" xlink:to="us-gaap_ChiefExecutiveOfficerMember" />
  </link:definitionLink>
</link:linkbase>
