<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019d27e6-0e6a-71b2-8ace-67a20efc29be,g:984b36f6-daaa-4660-8c7e-9cbb638cc741-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.statestreet.com/role/Cover" xlink:type="simple" xlink:href="stt-20260622.xsd#Cover"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/Cover" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationLineItems_019d27e8-64f1-762a-8623-2a4a56c402b3" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentInformationTable_019d27e8-64f1-792a-b636-3d36163dc532" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentInformationTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_dei_DocumentInformationLineItems_019d27e8-64f1-762a-8623-2a4a56c402b3" xlink:to="loc_dei_DocumentInformationTable_019d27e8-64f1-792a-b636-3d36163dc532" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27e8-64f1-74ed-92b7-bba3c9e79df6" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_dei_DocumentInformationTable_019d27e8-64f1-792a-b636-3d36163dc532" xlink:to="loc_dei_LegalEntityAxis_019d27e8-64f1-74ed-92b7-bba3c9e79df6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27e8-64f1-74ed-92b7-bba3c9e79df6_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27e8-64f1-74ed-92b7-bba3c9e79df6" xlink:to="loc_dei_EntityDomain_019d27e8-64f1-74ed-92b7-bba3c9e79df6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27e8-64f1-71d6-a804-ca42db5843e6" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27e8-64f1-74ed-92b7-bba3c9e79df6" xlink:to="loc_dei_EntityDomain_019d27e8-64f1-71d6-a804-ca42db5843e6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27e8-64f1-7ea1-9b82-ea7f1cbe6c14" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27e8-64f1-71d6-a804-ca42db5843e6" xlink:to="loc_stt_EBP002Member_019d27e8-64f1-7ea1-9b82-ea7f1cbe6c14" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_DocumentType_019d27e8-64f1-79e3-8472-7cd349436c36" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_DocumentType"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_019d27e8-64f1-762a-8623-2a4a56c402b3" xlink:to="loc_dei_DocumentType_019d27e8-64f1-79e3-8472-7cd349436c36" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityRegistrantName_019d27e8-64f1-7b04-bda6-5cf5803e9913" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityRegistrantName"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_019d27e8-64f1-762a-8623-2a4a56c402b3" xlink:to="loc_dei_EntityRegistrantName_019d27e8-64f1-7b04-bda6-5cf5803e9913" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_AmendmentFlag_019d27e8-64f1-7cf9-8f38-d2482d00f27f" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_AmendmentFlag"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_019d27e8-64f1-762a-8623-2a4a56c402b3" xlink:to="loc_dei_AmendmentFlag_019d27e8-64f1-7cf9-8f38-d2482d00f27f" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityCentralIndexKey_019d27e8-64f1-711e-a6cb-d2f43914d6fc" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityCentralIndexKey"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_DocumentInformationLineItems_019d27e8-64f1-762a-8623-2a4a56c402b3" xlink:to="loc_dei_EntityCentralIndexKey_019d27e8-64f1-711e-a6cb-d2f43914d6fc" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/StatementsofNetAssetsAvailableforBenefits" xlink:type="simple" xlink:href="stt-20260622.xsd#StatementsofNetAssetsAvailableforBenefits"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/StatementsofNetAssetsAvailableforBenefits" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitLineItems_019d27ed-9f5d-74d5-bd26-79a32fa3909e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitTable_019d27ed-9f5d-7d31-bc38-f87d0a5f6b31" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitLineItems_019d27ed-9f5d-74d5-bd26-79a32fa3909e" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitTable_019d27ed-9f5d-7d31-bc38-f87d0a5f6b31" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27ed-9f5d-78e0-be71-3839bbb81dcb" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitTable_019d27ed-9f5d-7d31-bc38-f87d0a5f6b31" xlink:to="loc_dei_LegalEntityAxis_019d27ed-9f5d-78e0-be71-3839bbb81dcb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ed-9f5d-78e0-be71-3839bbb81dcb_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27ed-9f5d-78e0-be71-3839bbb81dcb" xlink:to="loc_dei_EntityDomain_019d27ed-9f5d-78e0-be71-3839bbb81dcb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ed-9f5d-77a0-809d-78f829b29ff3" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27ed-9f5d-78e0-be71-3839bbb81dcb" xlink:to="loc_dei_EntityDomain_019d27ed-9f5d-77a0-809d-78f829b29ff3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27ed-9f5d-78df-b6bf-d67558d376d4" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27ed-9f5d-77a0-809d-78f829b29ff3" xlink:to="loc_stt_EBP002Member_019d27ed-9f5d-78df-b6bf-d67558d376d4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_019d27ed-9f5d-7db1-8f19-4ef0839b8115" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitTable_019d27ed-9f5d-7d31-bc38-f87d0a5f6b31" xlink:to="loc_us-gaap_InvestmentTypeAxis_019d27ed-9f5d-7db1-8f19-4ef0839b8115" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-7db1-8f19-4ef0839b8115_default" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d27ed-9f5d-7db1-8f19-4ef0839b8115" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-7db1-8f19-4ef0839b8115_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d27ed-9f5d-7db1-8f19-4ef0839b8115" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember_019d2c9b-0476-7133-ad9b-1e85832c41b2" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember_019d2c9b-0476-7133-ad9b-1e85832c41b2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPCommonCollectiveTrustShortTermInvestmentFundsMember_019d4b0a-71f8-79de-831f-955daa7cc24f" xlink:href="stt-20260622.xsd#stt_EBPCommonCollectiveTrustShortTermInvestmentFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:to="loc_stt_EBPCommonCollectiveTrustShortTermInvestmentFundsMember_019d4b0a-71f8-79de-831f-955daa7cc24f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPCommonCollectiveTrustExcludingShortTermInvestmentFundsMember_019d4b0a-0354-76e2-b66a-df45cfd6f90d" xlink:href="stt-20260622.xsd#stt_EBPCommonCollectiveTrustExcludingShortTermInvestmentFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:to="loc_stt_EBPCommonCollectiveTrustExcludingShortTermInvestmentFundsMember_019d4b0a-0354-76e2-b66a-df45cfd6f90d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d27ed-9f5d-7f1b-9166-e4f3e388ed95" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d27ed-9f5d-7f1b-9166-e4f3e388ed95" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_019d27ed-9f5d-7f4d-883b-496c9afa5d50" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27ed-9f5d-72a9-b063-921212a3b219" xlink:to="loc_us-gaap_MoneyMarketFundsMember_019d27ed-9f5d-7f4d-883b-496c9afa5d50" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAssetAbstract_019d27ed-9f5d-7b57-97e5-31785481a12c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAssetAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitLineItems_019d27ed-9f5d-74d5-bd26-79a32fa3909e" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAssetAbstract_019d27ed-9f5d-7b57-97e5-31785481a12c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAbstract_019d27ed-9f5d-712a-bdd7-29acf47bd3d9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAssetAbstract_019d27ed-9f5d-7b57-97e5-31785481a12c" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAbstract_019d27ed-9f5d-712a-bdd7-29acf47bd3d9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue_019d27ed-9f5d-76ea-b48d-41f00ba2085e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAbstract_019d27ed-9f5d-712a-bdd7-29acf47bd3d9" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue_019d27ed-9f5d-76ea-b48d-41f00ba2085e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant_019d27ed-9f5d-7e9f-bf9e-6700941e51b4" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAssetAbstract_019d27ed-9f5d-7b57-97e5-31785481a12c" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant_019d27ed-9f5d-7e9f-bf9e-6700941e51b4" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable_019d27ed-9f5d-739b-b0d2-1863956bf81f" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAssetAbstract_019d27ed-9f5d-7b57-97e5-31785481a12c" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable_019d27ed-9f5d-739b-b0d2-1863956bf81f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019d27ed-9f5d-79d8-af2a-834b8cfd94e3" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAsset"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAssetAbstract_019d27ed-9f5d-7b57-97e5-31785481a12c" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019d27ed-9f5d-79d8-af2a-834b8cfd94e3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanLiabilityAbstract_019d27ed-9f5d-75d7-9659-713eb87d84c8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanLiabilityAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitLineItems_019d27ed-9f5d-74d5-bd26-79a32fa3909e" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanLiabilityAbstract_019d27ed-9f5d-75d7-9659-713eb87d84c8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAccruedExpense_019d27ed-9f5d-7c42-bdba-592b7ae40205" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAccruedExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanLiabilityAbstract_019d27ed-9f5d-75d7-9659-713eb87d84c8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAccruedExpense_019d27ed-9f5d-7c42-bdba-592b7ae40205" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d27ed-9f5d-781c-a2b9-f0ca49055dab" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfNetAssetAvailableForBenefitLineItems_019d27ed-9f5d-74d5-bd26-79a32fa3909e" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d27ed-9f5d-781c-a2b9-f0ca49055dab" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/StatementofChangesinNetAssetsAvailableforBenefits" xlink:type="simple" xlink:href="stt-20260622.xsd#StatementofChangesinNetAssetsAvailableforBenefits"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/StatementofChangesinNetAssetsAvailableforBenefits" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems_019d27ef-85cb-7fc0-ae5f-dab32b3b9ac8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitTable_019d27ef-85cb-78ce-ab50-75e3223f137e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems_019d27ef-85cb-7fc0-ae5f-dab32b3b9ac8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitTable_019d27ef-85cb-78ce-ab50-75e3223f137e" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27ef-85cb-7e6c-99c3-3024eb84ab7e" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitTable_019d27ef-85cb-78ce-ab50-75e3223f137e" xlink:to="loc_dei_LegalEntityAxis_019d27ef-85cb-7e6c-99c3-3024eb84ab7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ef-85cb-7e6c-99c3-3024eb84ab7e_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27ef-85cb-7e6c-99c3-3024eb84ab7e" xlink:to="loc_dei_EntityDomain_019d27ef-85cb-7e6c-99c3-3024eb84ab7e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ef-85cb-7d2f-8354-170536e7864d" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27ef-85cb-7e6c-99c3-3024eb84ab7e" xlink:to="loc_dei_EntityDomain_019d27ef-85cb-7d2f-8354-170536e7864d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27ef-85cb-72bd-85be-d2766b162fc7" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27ef-85cb-7d2f-8354-170536e7864d" xlink:to="loc_stt_EBP002Member_019d27ef-85cb-72bd-85be-d2766b162fc7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems_019d27ef-85cb-7fc0-ae5f-dab32b3b9ac8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract_019d27ef-85cb-7970-aa3c-760889e2c7cf" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract_019d27ef-85cb-7970-aa3c-760889e2c7cf" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover_019d27ef-85cb-7e36-a436-1bf9850b6eeb" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract_019d27ef-85cb-7970-aa3c-760889e2c7cf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover_019d27ef-85cb-7e36-a436-1bf9850b6eeb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019d27ef-85cb-7b32-b17a-d9a7696a6f19" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract_019d27ef-85cb-7970-aa3c-760889e2c7cf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019d27ef-85cb-7b32-b17a-d9a7696a6f19" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionRollover_019d27ef-85cb-7ddf-a51b-1f85c1942a8d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionRollover"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract_019d27ef-85cb-7970-aa3c-760889e2c7cf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionRollover_019d27ef-85cb-7ddf-a51b-1f85c1942a8d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d27ef-85cb-7ce9-91a0-961bd9bc906b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContributionAbstract_019d27ef-85cb-7970-aa3c-760889e2c7cf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForContribution_019d27ef-85cb-7ce9-91a0-961bd9bc906b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant_019d27ef-85cb-72c6-af7a-12745cb6cb3c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant_019d27ef-85cb-72c6-af7a-12745cb6cb3c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment_019d27ef-85cb-7d96-b4fc-06b29e879ad9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment_019d27ef-85cb-7d96-b4fc-06b29e879ad9" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment_019d27ef-85cb-7aa8-8189-fa9892ef466c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment_019d27ef-85cb-7aa8-8189-fa9892ef466c" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d27ef-85cb-7694-8ef7-15feeb0a1d0c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019d27ef-85cb-7694-8ef7-15feeb0a1d0c" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d27ef-85cb-7bae-93ee-0b3dd7d8a4c3" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseAbstract_019d27ef-85cb-7238-93db-3e81bbd607ce" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019d27ef-85cb-7bae-93ee-0b3dd7d8a4c3" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseAbstract_019d27ef-85cb-78ae-a89d-8fe4d775bd6a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems_019d27ef-85cb-7fc0-ae5f-dab32b3b9ac8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseAbstract_019d27ef-85cb-78ae-a89d-8fe4d775bd6a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019d27ef-85cb-774b-ab17-9feb8178dee6" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseAbstract_019d27ef-85cb-78ae-a89d-8fe4d775bd6a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019d27ef-85cb-774b-ab17-9feb8178dee6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense_019d27ef-85cb-719b-8f82-3c6d72473a86" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseAbstract_019d27ef-85cb-78ae-a89d-8fe4d775bd6a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense_019d27ef-85cb-719b-8f82-3c6d72473a86" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d27ef-85cb-7370-b4e6-68ae91fc8d56" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseAbstract_019d27ef-85cb-78ae-a89d-8fe4d775bd6a" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019d27ef-85cb-7370-b4e6-68ae91fc8d56" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019d27ef-85cb-71a8-a216-5da5ca0f1b91" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems_019d27ef-85cb-7fc0-ae5f-dab32b3b9ac8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019d27ef-85cb-71a8-a216-5da5ca0f1b91" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d27ef-85cb-778b-86b0-962f9e2659f3" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanStatementOfChangeInNetAssetAvailableForBenefitLineItems_019d27ef-85cb-7fc0-ae5f-dab32b3b9ac8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d27ef-85cb-778b-86b0-962f9e2659f3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019d27ef-85cb-7938-be92-5d331674a158" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/DescriptionofthePlan" xlink:type="simple" xlink:href="stt-20260622.xsd#DescriptionofthePlan"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/DescriptionofthePlan" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-73b5-96bc-1e0fcddce0f3" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-70f6-be14-d61f0ed91175" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-73b5-96bc-1e0fcddce0f3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-70f6-be14-d61f0ed91175" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27ef-d91f-7955-b5e5-58ce98f0570b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-70f6-be14-d61f0ed91175" xlink:to="loc_dei_LegalEntityAxis_019d27ef-d91f-7955-b5e5-58ce98f0570b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ef-d91f-7955-b5e5-58ce98f0570b_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27ef-d91f-7955-b5e5-58ce98f0570b" xlink:to="loc_dei_EntityDomain_019d27ef-d91f-7955-b5e5-58ce98f0570b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ef-d91f-779d-b34f-b9fe66c58091" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27ef-d91f-7955-b5e5-58ce98f0570b" xlink:to="loc_dei_EntityDomain_019d27ef-d91f-779d-b34f-b9fe66c58091" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27ef-d91f-7646-ab6d-834303bfdb14" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27ef-d91f-779d-b34f-b9fe66c58091" xlink:to="loc_stt_EBP002Member_019d27ef-d91f-7646-ab6d-834303bfdb14" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTextBlock_019d27ef-d91f-7434-9823-87056718d626" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-73b5-96bc-1e0fcddce0f3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTextBlock_019d27ef-d91f-7434-9823-87056718d626" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/SignificantAccountingPolicies" xlink:type="simple" xlink:href="stt-20260622.xsd#SignificantAccountingPolicies"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/SignificantAccountingPolicies" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7db3-b162-ed02880a0318" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable_019d27f0-453c-7496-9357-3c23d57ac372" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7db3-b162-ed02880a0318" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable_019d27f0-453c-7496-9357-3c23d57ac372" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-453c-77bd-b0a7-e3ffc466c100" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable_019d27f0-453c-7496-9357-3c23d57ac372" xlink:to="loc_dei_LegalEntityAxis_019d27f0-453c-77bd-b0a7-e3ffc466c100" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-453c-77bd-b0a7-e3ffc466c100_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-453c-77bd-b0a7-e3ffc466c100" xlink:to="loc_dei_EntityDomain_019d27f0-453c-77bd-b0a7-e3ffc466c100_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-453c-729a-8b14-d9f9944d552b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-453c-77bd-b0a7-e3ffc466c100" xlink:to="loc_dei_EntityDomain_019d27f0-453c-729a-8b14-d9f9944d552b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-453c-700c-b9ac-bc5978e3b53d" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-453c-729a-8b14-d9f9944d552b" xlink:to="loc_stt_EBP002Member_019d27f0-453c-700c-b9ac-bc5978e3b53d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanSummaryOfAccountingPolicyTextBlock_019d27f0-453c-714d-a4cd-315dfc3ebcb0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanSummaryOfAccountingPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7db3-b162-ed02880a0318" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanSummaryOfAccountingPolicyTextBlock_019d27f0-453c-714d-a4cd-315dfc3ebcb0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/FairValueMeasurement" xlink:type="simple" xlink:href="stt-20260622.xsd#FairValueMeasurement"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/FairValueMeasurement" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-7946-ae23-09c10938e121" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-73f4-a17c-f68bd18a772a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-7946-ae23-09c10938e121" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-73f4-a17c-f68bd18a772a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-737e-78e0-be6d-066e743d19b6" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-73f4-a17c-f68bd18a772a" xlink:to="loc_dei_LegalEntityAxis_019d27f0-737e-78e0-be6d-066e743d19b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-737e-78e0-be6d-066e743d19b6_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-737e-78e0-be6d-066e743d19b6" xlink:to="loc_dei_EntityDomain_019d27f0-737e-78e0-be6d-066e743d19b6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-737e-7b7f-8afd-8a0eb1780919" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-737e-78e0-be6d-066e743d19b6" xlink:to="loc_dei_EntityDomain_019d27f0-737e-7b7f-8afd-8a0eb1780919" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-737e-793d-8dc2-a5da2c284a7a" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-737e-7b7f-8afd-8a0eb1780919" xlink:to="loc_stt_EBP002Member_019d27f0-737e-793d-8dc2-a5da2c284a7a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTextBlock_019d27f0-737e-7c47-a647-ec1803010388" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-7946-ae23-09c10938e121" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTextBlock_019d27f0-737e-7c47-a647-ec1803010388" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/TransactionsandAgreementswithPartiesinInterest" xlink:type="simple" xlink:href="stt-20260622.xsd#TransactionsandAgreementswithPartiesinInterest"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/TransactionsandAgreementswithPartiesinInterest" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-77cf-a08c-680522df1a87" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-77ed-a5cf-2be631c66f81" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-77cf-a08c-680522df1a87" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-77ed-a5cf-2be631c66f81" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-d84f-705e-aa6a-3e567825f3ac" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-77ed-a5cf-2be631c66f81" xlink:to="loc_dei_LegalEntityAxis_019d27f0-d84f-705e-aa6a-3e567825f3ac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-d84f-705e-aa6a-3e567825f3ac_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-d84f-705e-aa6a-3e567825f3ac" xlink:to="loc_dei_EntityDomain_019d27f0-d84f-705e-aa6a-3e567825f3ac_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-d84f-7146-93c7-d6776766763f" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-d84f-705e-aa6a-3e567825f3ac" xlink:to="loc_dei_EntityDomain_019d27f0-d84f-7146-93c7-d6776766763f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-d84f-7e81-9895-cc0879c29107" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-d84f-7146-93c7-d6776766763f" xlink:to="loc_stt_EBP002Member_019d27f0-d84f-7e81-9895-cc0879c29107" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTextBlock_019d27f0-d84f-7807-96c4-6c7648d4fd85" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-77cf-a08c-680522df1a87" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTextBlock_019d27f0-d84f-7807-96c4-6c7648d4fd85" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/RisksandUncertainties" xlink:type="simple" xlink:href="stt-20260622.xsd#RisksandUncertainties"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/RisksandUncertainties" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EbpRiskAndUncertaintyLineItems_019d27f1-27b0-710c-8bcb-b4cf2af31203" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EbpRiskAndUncertaintyLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EbpRiskAndUncertaintyTable_019d27f1-27b0-7a83-bf41-3350b3f29621" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EbpRiskAndUncertaintyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EbpRiskAndUncertaintyLineItems_019d27f1-27b0-710c-8bcb-b4cf2af31203" xlink:to="loc_us-gaap-ebp_EbpRiskAndUncertaintyTable_019d27f1-27b0-7a83-bf41-3350b3f29621" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f1-27b0-77bb-b3fc-a76391d5e61b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EbpRiskAndUncertaintyTable_019d27f1-27b0-7a83-bf41-3350b3f29621" xlink:to="loc_dei_LegalEntityAxis_019d27f1-27b0-77bb-b3fc-a76391d5e61b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f1-27b0-77bb-b3fc-a76391d5e61b_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f1-27b0-77bb-b3fc-a76391d5e61b" xlink:to="loc_dei_EntityDomain_019d27f1-27b0-77bb-b3fc-a76391d5e61b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f1-27b0-792d-a910-aa1f9023363b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f1-27b0-77bb-b3fc-a76391d5e61b" xlink:to="loc_dei_EntityDomain_019d27f1-27b0-792d-a910-aa1f9023363b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f1-27b0-77f6-a06c-0ca336ac171a" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f1-27b0-792d-a910-aa1f9023363b" xlink:to="loc_stt_EBP002Member_019d27f1-27b0-77f6-a06c-0ca336ac171a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanRiskAndUncertaintyTextBlock_019d27f1-27b0-7017-8587-c5a854717cde" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanRiskAndUncertaintyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EbpRiskAndUncertaintyLineItems_019d27f1-27b0-710c-8bcb-b4cf2af31203" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanRiskAndUncertaintyTextBlock_019d27f1-27b0-7017-8587-c5a854717cde" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/IncomeTaxStatus" xlink:type="simple" xlink:href="stt-20260622.xsd#IncomeTaxStatus"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/IncomeTaxStatus" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusLineItems_019d27f1-5cd2-7fa7-8751-91a10d0e973c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanTaxStatusLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusTable_019d27f1-5cd2-7e23-966a-181b3e596f70" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanTaxStatusTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusLineItems_019d27f1-5cd2-7fa7-8751-91a10d0e973c" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusTable_019d27f1-5cd2-7e23-966a-181b3e596f70" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f1-5cd2-7615-8e75-ccf34f478318" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusTable_019d27f1-5cd2-7e23-966a-181b3e596f70" xlink:to="loc_dei_LegalEntityAxis_019d27f1-5cd2-7615-8e75-ccf34f478318" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f1-5cd2-7615-8e75-ccf34f478318_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f1-5cd2-7615-8e75-ccf34f478318" xlink:to="loc_dei_EntityDomain_019d27f1-5cd2-7615-8e75-ccf34f478318_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f1-5cd2-7063-8878-954bd27e24d9" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f1-5cd2-7615-8e75-ccf34f478318" xlink:to="loc_dei_EntityDomain_019d27f1-5cd2-7063-8878-954bd27e24d9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f1-5cd2-77c3-bf7b-9b3cccff5c2b" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f1-5cd2-7063-8878-954bd27e24d9" xlink:to="loc_stt_EBP002Member_019d27f1-5cd2-77c3-bf7b-9b3cccff5c2b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusTextBlock_019d27f1-5cd2-7bbe-bdf4-e270a0318f67" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanTaxStatusTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusLineItems_019d27f1-5cd2-7fa7-8751-91a10d0e973c" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanTaxStatusTextBlock_019d27f1-5cd2-7bbe-bdf4-e270a0318f67" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/ScheduleHLine4iScheduleofAssetsHeldAtEndofYear" xlink:type="simple" xlink:href="stt-20260622.xsd#ScheduleHLine4iScheduleofAssetsHeldAtEndofYear"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/ScheduleHLine4iScheduleofAssetsHeldAtEndofYear" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-72c4-8095-071e23fc5e30" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-73b3-88c6-4c2039f19f52" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-72c4-8095-071e23fc5e30" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-73b3-88c6-4c2039f19f52" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f2-142a-7ad1-a42a-27d32fad36b6" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-73b3-88c6-4c2039f19f52" xlink:to="loc_dei_LegalEntityAxis_019d27f2-142a-7ad1-a42a-27d32fad36b6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f2-142a-7ad1-a42a-27d32fad36b6_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f2-142a-7ad1-a42a-27d32fad36b6" xlink:to="loc_dei_EntityDomain_019d27f2-142a-7ad1-a42a-27d32fad36b6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f2-142a-73a7-a351-84b5ec47bdf5" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f2-142a-7ad1-a42a-27d32fad36b6" xlink:to="loc_dei_EntityDomain_019d27f2-142a-73a7-a351-84b5ec47bdf5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f2-142a-7776-9386-fd2e2efe3db6" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f2-142a-73a7-a351-84b5ec47bdf5" xlink:to="loc_stt_EBP002Member_019d27f2-142a-7776-9386-fd2e2efe3db6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTableTextBlock_019d27f2-142a-7280-9203-9e99dc076070" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-72c4-8095-071e23fc5e30" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTableTextBlock_019d27f2-142a-7280-9203-9e99dc076070" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/SignificantAccountingPoliciesPolicies" xlink:type="simple" xlink:href="stt-20260622.xsd#SignificantAccountingPoliciesPolicies"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/SignificantAccountingPoliciesPolicies" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable_019d27f0-453c-7e91-b897-743e43e8f668" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable_019d27f0-453c-7e91-b897-743e43e8f668" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-453c-7591-9791-0737d0650175" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyTable_019d27f0-453c-7e91-b897-743e43e8f668" xlink:to="loc_dei_LegalEntityAxis_019d27f0-453c-7591-9791-0737d0650175" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-453c-7591-9791-0737d0650175_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-453c-7591-9791-0737d0650175" xlink:to="loc_dei_EntityDomain_019d27f0-453c-7591-9791-0737d0650175_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-453c-7d06-8c8c-d3d4598dd168" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-453c-7591-9791-0737d0650175" xlink:to="loc_dei_EntityDomain_019d27f0-453c-7d06-8c8c-d3d4598dd168" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-453c-7398-9a60-362a2e117f21" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-453c-7d06-8c8c-d3d4598dd168" xlink:to="loc_stt_EBP002Member_019d27f0-453c-7398-9a60-362a2e117f21" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanBasisOfAccountingPolicyTextBlock_019d27f0-453c-7e7a-89f6-c41508adc6fe" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanBasisOfAccountingPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanBasisOfAccountingPolicyTextBlock_019d27f0-453c-7e7a-89f6-c41508adc6fe" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentPolicyTextBlock_019d27f0-453c-75bb-8d09-ddcc987c6415" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentPolicyTextBlock_019d27f0-453c-75bb-8d09-ddcc987c6415" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanPaymentToParticipantPolicyTextBlock_019d27f0-453c-70c0-8f92-b3621e76adba" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanPaymentToParticipantPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanPaymentToParticipantPolicyTextBlock_019d27f0-453c-70c0-8f92-b3621e76adba" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantPolicyTextBlock_019d2d26-bcae-7efb-b3af-7bb263b56d5b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantPolicyTextBlock_019d2d26-bcae-7efb-b3af-7bb263b56d5b" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanUseOfEstimatePolicyTextBlock_019d27f0-453c-7b68-9a75-c7764d89d4da" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanUseOfEstimatePolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanUseOfEstimatePolicyTextBlock_019d27f0-453c-7b68-9a75-c7764d89d4da" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanContributionPolicyTextBlock_019d2d27-239b-7ad9-a9f3-92e1c1f4a687" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanContributionPolicyTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAccountingPolicyLineItems_019d27f0-453c-7c44-bfa8-b3d291a1b028" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanContributionPolicyTextBlock_019d2d27-239b-7ad9-a9f3-92e1c1f4a687" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/FairValueMeasurementTables" xlink:type="simple" xlink:href="stt-20260622.xsd#FairValueMeasurementTables"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/FairValueMeasurementTables" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-733a-ae80-e57a819f80c9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-743d-953e-9a58e13ad391" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-733a-ae80-e57a819f80c9" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-743d-953e-9a58e13ad391" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-737e-7802-815c-89b336f3804c" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-743d-953e-9a58e13ad391" xlink:to="loc_dei_LegalEntityAxis_019d27f0-737e-7802-815c-89b336f3804c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-737e-7802-815c-89b336f3804c_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-737e-7802-815c-89b336f3804c" xlink:to="loc_dei_EntityDomain_019d27f0-737e-7802-815c-89b336f3804c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-737e-7851-88bb-b3d02c7e7776" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-737e-7802-815c-89b336f3804c" xlink:to="loc_dei_EntityDomain_019d27f0-737e-7851-88bb-b3d02c7e7776" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-737e-7e02-8680-307825d3577d" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-737e-7851-88bb-b3d02c7e7776" xlink:to="loc_stt_EBP002Member_019d27f0-737e-7e02-8680-307825d3577d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTableTextBlock_019d27f0-737e-7c84-9b2a-dd0f8508dfa1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTableTextBlock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-733a-ae80-e57a819f80c9" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTableTextBlock_019d27f0-737e-7c84-9b2a-dd0f8508dfa1" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/DescriptionofthePlanDetails" xlink:type="simple" xlink:href="stt-20260622.xsd#DescriptionofthePlanDetails"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/DescriptionofthePlanDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-75a9-83f9-a0fb3570c7ff" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-75a9-83f9-a0fb3570c7ff" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27ef-d91f-736f-8a6e-7a04e498167b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-75a9-83f9-a0fb3570c7ff" xlink:to="loc_dei_LegalEntityAxis_019d27ef-d91f-736f-8a6e-7a04e498167b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ef-d91f-736f-8a6e-7a04e498167b_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27ef-d91f-736f-8a6e-7a04e498167b" xlink:to="loc_dei_EntityDomain_019d27ef-d91f-736f-8a6e-7a04e498167b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27ef-d91f-7abf-8a5e-f255b1b26be1" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27ef-d91f-736f-8a6e-7a04e498167b" xlink:to="loc_dei_EntityDomain_019d27ef-d91f-7abf-8a5e-f255b1b26be1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27ef-d91f-77bc-bb67-8fce276d5cd8" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27ef-d91f-7abf-8a5e-f255b1b26be1" xlink:to="loc_stt_EBP002Member_019d27ef-d91f-77bc-bb67-8fce276d5cd8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019d27ef-d91f-7e85-b67b-da8a0272e43b" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanTable_019d27ef-d91f-75a9-83f9-a0fb3570c7ff" xlink:to="loc_srt_RangeAxis_019d27ef-d91f-7e85-b67b-da8a0272e43b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019d27ef-d91f-7e85-b67b-da8a0272e43b_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019d27ef-d91f-7e85-b67b-da8a0272e43b" xlink:to="loc_srt_RangeMember_019d27ef-d91f-7e85-b67b-da8a0272e43b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019d27ef-d91f-7298-8f1a-ce57be19f7c1" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019d27ef-d91f-7e85-b67b-da8a0272e43b" xlink:to="loc_srt_RangeMember_019d27ef-d91f-7298-8f1a-ce57be19f7c1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019d27ef-d91f-7424-b951-d5faace25721" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019d27ef-d91f-7298-8f1a-ce57be19f7c1" xlink:to="loc_srt_MinimumMember_019d27ef-d91f-7424-b951-d5faace25721" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019d27ef-d91f-71dd-b08b-aa703545e8ae" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019d27ef-d91f-7298-8f1a-ce57be19f7c1" xlink:to="loc_srt_MaximumMember_019d27ef-d91f-71dd-b08b-aa703545e8ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPParticipantContributionMinimumContributionToEligibleCompensationPercentage_019d27ef-d91f-758b-aac3-6d252a3cc4bf" xlink:href="stt-20260622.xsd#stt_EBPParticipantContributionMinimumContributionToEligibleCompensationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPParticipantContributionMinimumContributionToEligibleCompensationPercentage_019d27ef-d91f-758b-aac3-6d252a3cc4bf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantContributionPretaxMaximumAnnualCompensationToTotalCompensationPercentage_019d2d1a-7df9-7f36-8d78-f405244fb273" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanParticipantContributionPretaxMaximumAnnualCompensationToTotalCompensationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantContributionPretaxMaximumAnnualCompensationToTotalCompensationPercentage_019d2d1a-7df9-7f36-8d78-f405244fb273" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantContributionAutomaticDeferralRate_019d2cff-c5e9-7ec7-9267-6ff5f280a175" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanParticipantContributionAutomaticDeferralRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantContributionAutomaticDeferralRate_019d2cff-c5e9-7ec7-9267-6ff5f280a175" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPParticipantContributionAutomaticAnnualIncreaseDeferralRate_019d2d00-01b1-7b14-b544-8aad2a93cd62" xlink:href="stt-20260622.xsd#stt_EBPParticipantContributionAutomaticAnnualIncreaseDeferralRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPParticipantContributionAutomaticAnnualIncreaseDeferralRate_019d2d00-01b1-7b14-b544-8aad2a93cd62" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionMatchingPercentage_019d2d01-5fad-76d2-a6b0-4b0b6e1185fa" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerContributionMatchingPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionMatchingPercentage_019d2d01-5fad-76d2-a6b0-4b0b6e1185fa" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionParticipantCompensationMatchedPercentage_019d2d01-8b8c-7b57-801b-51a03104e01b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerContributionParticipantCompensationMatchedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionParticipantCompensationMatchedPercentage_019d2d01-8b8c-7b57-801b-51a03104e01b" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPEmployerContributionVestingServicePeriod_019d2d04-43d2-7f93-927f-d25bacda3ecc" xlink:href="stt-20260622.xsd#stt_EBPEmployerContributionVestingServicePeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPEmployerContributionVestingServicePeriod_019d2d04-43d2-7f93-927f-d25bacda3ecc" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPEmployerContributionVestingPercentage_019d2d02-5dab-7295-a036-8e146bb78c9e" xlink:href="stt-20260622.xsd#stt_EBPEmployerContributionVestingPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPEmployerContributionVestingPercentage_019d2d02-5dab-7295-a036-8e146bb78c9e" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPParticipantContributionInvestmentLimitationMaximumPercentageOfAccountBalance_019d2d08-2c40-7d4d-9b17-4f1dee673215" xlink:href="stt-20260622.xsd#stt_EBPParticipantContributionInvestmentLimitationMaximumPercentageOfAccountBalance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPParticipantContributionInvestmentLimitationMaximumPercentageOfAccountBalance_019d2d08-2c40-7d4d-9b17-4f1dee673215" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPInvoluntaryRolloverLimit_019d2d18-7a64-7f6e-a5b0-46cee2fd2cc0" xlink:href="stt-20260622.xsd#stt_EBPInvoluntaryRolloverLimit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPInvoluntaryRolloverLimit_019d2d18-7a64-7f6e-a5b0-46cee2fd2cc0" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPInvoluntaryCashOutLimit_019d2d17-29be-7a88-97ae-c1d320ece540" xlink:href="stt-20260622.xsd#stt_EBPInvoluntaryCashOutLimit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPInvoluntaryCashOutLimit_019d2d17-29be-7a88-97ae-c1d320ece540" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPRetirementInstallmentPaymentMinimumAgeAtRetirement_019e8f90-d405-7bd4-a45e-4eb7c58912af" xlink:href="stt-20260622.xsd#stt_EBPRetirementInstallmentPaymentMinimumAgeAtRetirement"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPRetirementInstallmentPaymentMinimumAgeAtRetirement_019e8f90-d405-7bd4-a45e-4eb7c58912af" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPRetirementInstallmentPaymentYearsOfService_019d4b10-079d-71c9-9cec-cf1670cf204d" xlink:href="stt-20260622.xsd#stt_EBPRetirementInstallmentPaymentYearsOfService"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPRetirementInstallmentPaymentYearsOfService_019d4b10-079d-71c9-9cec-cf1670cf204d" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMinimumBorrowingAmount_019d2d1f-89b3-7b97-91f0-1178da3e0d69" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMinimumBorrowingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMinimumBorrowingAmount_019d2d1f-89b3-7b97-91f0-1178da3e0d69" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMaximumBorrowingToTotalAccountPercentage_019d2d22-b142-7f10-b552-2833b2471df7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMaximumBorrowingToTotalAccountPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMaximumBorrowingToTotalAccountPercentage_019d2d22-b142-7f10-b552-2833b2471df7" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMaximumBorrowingAmount_019d2d1f-bb6b-7cc5-bd73-8205f6642e8e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMaximumBorrowingAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipantAccountMaximumBorrowingAmount_019d2d1f-bb6b-7cc5-bd73-8205f6642e8e" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPNoteReceivableFromParticipantGeneralLoanInterestRateBasisSpreadOnVariableRate_019e8f86-e400-7fab-b67b-0ca09d1aa038" xlink:href="stt-20260622.xsd#stt_EBPNoteReceivableFromParticipantGeneralLoanInterestRateBasisSpreadOnVariableRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPNoteReceivableFromParticipantGeneralLoanInterestRateBasisSpreadOnVariableRate_019e8f86-e400-7fab-b67b-0ca09d1aa038" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPNoteReceivableFromParticipantGeneralLoanMaximumBorrowingTerm_019d2d20-dce4-7559-b8d4-9ae75195d604" xlink:href="stt-20260622.xsd#stt_EBPNoteReceivableFromParticipantGeneralLoanMaximumBorrowingTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPNoteReceivableFromParticipantGeneralLoanMaximumBorrowingTerm_019d2d20-dce4-7559-b8d4-9ae75195d604" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPNoteReceivableFromParticipantAccountPrimaryResidenceBasisSpreadOnVariableRate_019e8f88-55e1-76ce-bec4-c195ff2c86a0" xlink:href="stt-20260622.xsd#stt_EBPNoteReceivableFromParticipantAccountPrimaryResidenceBasisSpreadOnVariableRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPNoteReceivableFromParticipantAccountPrimaryResidenceBasisSpreadOnVariableRate_019e8f88-55e1-76ce-bec4-c195ff2c86a0" xlink:type="arc" order="18"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBPNoteReceivableFromParticipantAccountPrimaryResidenceMaximumBorrowingTerm_019d2d21-20c3-754a-80dd-98ca2b9654d8" xlink:href="stt-20260622.xsd#stt_EBPNoteReceivableFromParticipantAccountPrimaryResidenceMaximumBorrowingTerm"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanDescriptionOfPlanLineItems_019d27ef-d91f-7420-9245-7d8814e4afe8" xlink:to="loc_stt_EBPNoteReceivableFromParticipantAccountPrimaryResidenceMaximumBorrowingTerm_019d2d21-20c3-754a-80dd-98ca2b9654d8" xlink:type="arc" order="19"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/FairValueMeasurementDetails" xlink:type="simple" xlink:href="stt-20260622.xsd#FairValueMeasurementDetails"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/FairValueMeasurementDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-769c-94c7-c0bdf2812645" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-7dc0-a144-f0a56768e6f5" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-769c-94c7-c0bdf2812645" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-7dc0-a144-f0a56768e6f5" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-737e-736f-9195-2e8fc4063a22" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-7dc0-a144-f0a56768e6f5" xlink:to="loc_dei_LegalEntityAxis_019d27f0-737e-736f-9195-2e8fc4063a22" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-737e-736f-9195-2e8fc4063a22_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-737e-736f-9195-2e8fc4063a22" xlink:to="loc_dei_EntityDomain_019d27f0-737e-736f-9195-2e8fc4063a22_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-737e-7f56-8479-741791d51159" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-737e-736f-9195-2e8fc4063a22" xlink:to="loc_dei_EntityDomain_019d27f0-737e-7f56-8479-741791d51159" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-737e-773e-ac5d-89308ce9a614" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-737e-7f56-8479-741791d51159" xlink:to="loc_stt_EBP002Member_019d27f0-737e-773e-ac5d-89308ce9a614" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019d27f0-737e-7fea-b188-9382269f1bd5" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-7dc0-a144-f0a56768e6f5" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019d27f0-737e-7fea-b188-9382269f1bd5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-7fea-b188-9382269f1bd5_default" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019d27f0-737e-7fea-b188-9382269f1bd5" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-7fea-b188-9382269f1bd5_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-71d1-9299-fc5ce719ab9f" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_019d27f0-737e-7fea-b188-9382269f1bd5" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-71d1-9299-fc5ce719ab9f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_019d27f0-737e-7ec5-a0a0-e48235d281b0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-71d1-9299-fc5ce719ab9f" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_019d27f0-737e-7ec5-a0a0-e48235d281b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_019d27f0-737e-7eb1-ab79-91d8bab02dcd" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-71d1-9299-fc5ce719ab9f" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_019d27f0-737e-7eb1-ab79-91d8bab02dcd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel3Member_019d2d2b-bb30-7090-a78d-e1d242ab62d5" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueInputsLevel3Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_019d27f0-737e-71d1-9299-fc5ce719ab9f" xlink:to="loc_us-gaap_FairValueInputsLevel3Member_019d2d2b-bb30-7090-a78d-e1d242ab62d5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_019d27f0-737e-7d2a-81a2-0215137ab707" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavTable_019d27f0-737e-7dc0-a144-f0a56768e6f5" xlink:to="loc_us-gaap_InvestmentTypeAxis_019d27f0-737e-7d2a-81a2-0215137ab707" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7d2a-81a2-0215137ab707_default" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d27f0-737e-7d2a-81a2-0215137ab707" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7d2a-81a2-0215137ab707_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7ace-92a7-09617a83d9b8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d27f0-737e-7d2a-81a2-0215137ab707" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7ace-92a7-09617a83d9b8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DefinedBenefitPlanCommonCollectiveTrustMember_019d4b0b-77cd-7656-8d29-686f182d15f7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DefinedBenefitPlanCommonCollectiveTrustMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7ace-92a7-09617a83d9b8" xlink:to="loc_us-gaap_DefinedBenefitPlanCommonCollectiveTrustMember_019d4b0b-77cd-7656-8d29-686f182d15f7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember_019d2d2d-6b9e-7006-a268-dc4406260b66" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7ace-92a7-09617a83d9b8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember_019d2d2d-6b9e-7006-a268-dc4406260b66" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_019d27f0-737e-7029-a09c-995b048397bd" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7ace-92a7-09617a83d9b8" xlink:to="loc_us-gaap_MoneyMarketFundsMember_019d27f0-737e-7029-a09c-995b048397bd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d27f0-737e-714c-9019-f6aac97525fe" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-737e-7ace-92a7-09617a83d9b8" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d27f0-737e-714c-9019-f6aac97525fe" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue_019d27f0-737e-741d-8dfc-176a703cbaa3" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValueAndNavLineItems_019d27f0-737e-769c-94c7-c0bdf2812645" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentExcludingPlanInterestInMasterTrustFairValue_019d27f0-737e-741d-8dfc-176a703cbaa3" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/TransactionsandAgreementswithPartiesinInterestDetails" xlink:type="simple" xlink:href="stt-20260622.xsd#TransactionsandAgreementswithPartiesinInterestDetails"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/TransactionsandAgreementswithPartiesinInterestDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-741b-b93c-204eafe5ca93" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-7b0a-a16f-c219bb05bb5f" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-741b-b93c-204eafe5ca93" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-7b0a-a16f-c219bb05bb5f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f0-d84f-750d-b22f-5f28a3d63ea1" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-7b0a-a16f-c219bb05bb5f" xlink:to="loc_dei_LegalEntityAxis_019d27f0-d84f-750d-b22f-5f28a3d63ea1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-d84f-750d-b22f-5f28a3d63ea1_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f0-d84f-750d-b22f-5f28a3d63ea1" xlink:to="loc_dei_EntityDomain_019d27f0-d84f-750d-b22f-5f28a3d63ea1_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f0-d84f-7968-a895-0ce6b387e314" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f0-d84f-750d-b22f-5f28a3d63ea1" xlink:to="loc_dei_EntityDomain_019d27f0-d84f-7968-a895-0ce6b387e314" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f0-d84f-7927-b820-9700caba0811" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f0-d84f-7968-a895-0ce6b387e314" xlink:to="loc_stt_EBP002Member_019d27f0-d84f-7927-b820-9700caba0811" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_019d27f0-d84f-7fd4-a975-7d087b275ae6" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsTable_019d27f0-d84f-7b0a-a16f-c219bb05bb5f" xlink:to="loc_us-gaap_InvestmentTypeAxis_019d27f0-d84f-7fd4-a975-7d087b275ae6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-d84f-7fd4-a975-7d087b275ae6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d27f0-d84f-7fd4-a975-7d087b275ae6" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-d84f-7fd4-a975-7d087b275ae6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-d84f-7539-b964-6e5315092622" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d27f0-d84f-7fd4-a975-7d087b275ae6" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-d84f-7539-b964-6e5315092622" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember_019d27f0-d84f-7216-9c47-bf7966470803" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d27f0-d84f-7539-b964-6e5315092622" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerCommonStockMember_019d27f0-d84f-7216-9c47-bf7966470803" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForDividendIncomeOnInvestment_019d2d33-4ab3-78e1-9bb6-62000ff942f7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForDividendIncomeOnInvestment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-741b-b93c-204eafe5ca93" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForDividendIncomeOnInvestment_019d2d33-4ab3-78e1-9bb6-62000ff942f7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable_019d2d36-aaf4-7703-8434-119789fd7359" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanRelatedPartyAndPartyInInterestTransactionsLineItems_019d27f0-d84f-741b-b93c-204eafe5ca93" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable_019d2d36-aaf4-7703-8434-119789fd7359" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/ScheduleHLine4iScheduleofAssetsHeldAtEndofYearDetails" xlink:type="simple" xlink:href="stt-20260622.xsd#ScheduleHLine4iScheduleofAssetsHeldAtEndofYearDetails"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/ScheduleHLine4iScheduleofAssetsHeldAtEndofYearDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f2-142a-7b21-bf08-9d8c44793a36_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:to="loc_dei_EntityDomain_019d27f2-142a-7b21-bf08-9d8c44793a36_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f2-142a-7e55-8e11-b6158806a19b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:to="loc_dei_EntityDomain_019d27f2-142a-7e55-8e11-b6158806a19b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f2-142a-79fe-96d0-ab9f00c44e41" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f2-142a-7e55-8e11-b6158806a19b" xlink:to="loc_stt_EBP002Member_019d27f2-142a-79fe-96d0-ab9f00c44e41" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentIdentifierAxis_019d27f2-142a-7607-832c-d4b2f928665a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentIdentifierAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_us-gaap_InvestmentIdentifierAxis_019d27f2-142a-7607-832c-d4b2f928665a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019d27f2-142a-7c3a-991e-bdc4754c7141_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:to="loc_srt_RangeMember_019d27f2-142a-7c3a-991e-bdc4754c7141_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:to="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019d27f2-142a-75a8-8684-419721f1972a" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:to="loc_srt_MinimumMember_019d27f2-142a-75a8-8684-419721f1972a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019d27f2-142a-7038-a821-a529ed0c9471" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:to="loc_srt_MaximumMember_019d27f2-142a-7038-a821-a529ed0c9471" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7b01-bf67-d1e133fff9b4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7b01-bf67-d1e133fff9b4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7d92-8f7b-8ac60ed14b8d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7d92-8f7b-8ac60ed14b8d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d4b16-96e0-7119-a731-457e964b3b8b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7d92-8f7b-8ac60ed14b8d" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d4b16-96e0-7119-a731-457e964b3b8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_019d27f2-142a-7f97-8a19-0c20820db0fb" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_dei_EntityTaxIdentificationNumber_019d27f2-142a-7f97-8a19-0c20820db0fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanPlanNumber_019d27f2-142a-7bfa-8f62-f3aedd41a2ae" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanPlanNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanPlanNumber_019d27f2-142a-7bfa-8f62-f3aedd41a2ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentNumberOfShares_019d27f2-142a-742b-8ef5-b2a1839e3e95" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentNumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentNumberOfShares_019d27f2-142a-742b-8ef5-b2a1839e3e95" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantLoanInterestRate_019d27f2-142a-73c1-affc-0b5046ae954e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanParticipantLoanInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantLoanInterestRate_019d27f2-142a-73c1-affc-0b5046ae954e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentInvestmentExcludingPlanInterestInMasterTrustCurrentValue_019d27f2-142a-7541-bcec-c3940553b6d6" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentInvestmentExcludingPlanInterestInMasterTrustCurrentValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentInvestmentExcludingPlanInterestInMasterTrustCurrentValue_019d27f2-142a-7541-bcec-c3940553b6d6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentParticipantLoanAfterDeemedDistribution_019d27f2-142a-732d-b7c5-47a8bb0fb766" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentParticipantLoanAfterDeemedDistribution"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentParticipantLoanAfterDeemedDistribution_019d27f2-142a-732d-b7c5-47a8bb0fb766" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.statestreet.com/role/ScheduleHLine4iScheduleofAssetsHeldAtEndofYearDetails_1" xlink:type="simple" xlink:href="stt-20260622.xsd#ScheduleHLine4iScheduleofAssetsHeldAtEndofYearDetails_1"/>
  <link:definitionLink xlink:role="http://www.statestreet.com/role/ScheduleHLine4iScheduleofAssetsHeldAtEndofYearDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_LegalEntityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f2-142a-7b21-bf08-9d8c44793a36_default" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:to="loc_dei_EntityDomain_019d27f2-142a-7b21-bf08-9d8c44793a36_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityDomain_019d27f2-142a-7e55-8e11-b6158806a19b" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_dei_LegalEntityAxis_019d27f2-142a-7b21-bf08-9d8c44793a36" xlink:to="loc_dei_EntityDomain_019d27f2-142a-7e55-8e11-b6158806a19b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_stt_EBP002Member_019d27f2-142a-79fe-96d0-ab9f00c44e41" xlink:href="stt-20260622.xsd#stt_EBP002Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_dei_EntityDomain_019d27f2-142a-7e55-8e11-b6158806a19b" xlink:to="loc_stt_EBP002Member_019d27f2-142a-79fe-96d0-ab9f00c44e41" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019d27f2-142a-7c3a-991e-bdc4754c7141_default" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:to="loc_srt_RangeMember_019d27f2-142a-7c3a-991e-bdc4754c7141_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_019d27f2-142a-7c3a-991e-bdc4754c7141" xlink:to="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_019d27f2-142a-75a8-8684-419721f1972a" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:to="loc_srt_MinimumMember_019d27f2-142a-75a8-8684-419721f1972a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_019d27f2-142a-7038-a821-a529ed0c9471" xlink:href="https://xbrl.fasb.org/srt/2025/elts/srt-2025.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_019d27f2-142a-7405-8ff7-3a16b9948f76" xlink:to="loc_srt_MaximumMember_019d27f2-142a-7038-a821-a529ed0c9471" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentTable_019d27f2-142a-7b23-bcd1-6839940cf53f" xlink:to="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7b01-bf67-d1e133fff9b4_default" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7b01-bf67-d1e133fff9b4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7d92-8f7b-8ac60ed14b8d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InvestmentTypeCategorizationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_InvestmentTypeAxis_019d4b16-96e0-7b01-bf67-d1e133fff9b4" xlink:to="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7d92-8f7b-8ac60ed14b8d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d4b16-96e0-7119-a731-457e964b3b8b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_InvestmentTypeCategorizationMember_019d4b16-96e0-7d92-8f7b-8ac60ed14b8d" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanSelfDirectedBrokerageAccountMember_019d4b16-96e0-7119-a731-457e964b3b8b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_dei_EntityTaxIdentificationNumber_019d27f2-142a-7f97-8a19-0c20820db0fb" xlink:href="https://xbrl.sec.gov/dei/2025/dei-2025.xsd#dei_EntityTaxIdentificationNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_dei_EntityTaxIdentificationNumber_019d27f2-142a-7f97-8a19-0c20820db0fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanPlanNumber_019d27f2-142a-7bfa-8f62-f3aedd41a2ae" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanPlanNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanPlanNumber_019d27f2-142a-7bfa-8f62-f3aedd41a2ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentNumberOfShares_019d27f2-142a-742b-8ef5-b2a1839e3e95" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentNumberOfShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentNumberOfShares_019d27f2-142a-742b-8ef5-b2a1839e3e95" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantLoanInterestRate_019d27f2-142a-73c1-affc-0b5046ae954e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanParticipantLoanInterestRate"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantLoanInterestRate_019d27f2-142a-73c1-affc-0b5046ae954e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentInvestmentExcludingPlanInterestInMasterTrustCurrentValue_019d27f2-142a-7541-bcec-c3940553b6d6" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentInvestmentExcludingPlanInterestInMasterTrustCurrentValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentInvestmentExcludingPlanInterestInMasterTrustCurrentValue_019d27f2-142a-7541-bcec-c3940553b6d6" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentParticipantLoanAfterDeemedDistribution_019d27f2-142a-732d-b7c5-47a8bb0fb766" xlink:href="https://xbrl.fasb.org/us-gaap/2025/ebp/elts/us-gaap-ebp-2025.xsd#us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentParticipantLoanAfterDeemedDistribution"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanScheduleOfAssetHeldForInvestmentLineItems_019d27f2-142a-7231-8a20-65aeedd0d2d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAssetHeldForInvestmentParticipantLoanAfterDeemedDistribution_019d27f2-142a-732d-b7c5-47a8bb0fb766" xlink:type="arc" order="5"/>
  </link:definitionLink>
</link:linkbase>
