Filed Pursuant to Rule 424(b)(7)

Registration No. 333-286686

PROSPECTUS SUPPLEMENT NO. 1

(To Prospectus dated April 29, 2025)

16,894,212 Shares of Common Stock

This prospectus supplement supplements the prospectus dated April 29, 2025, relating to the resale by the selling stockholders identified in the prospectus (each, a “Selling Stockholder,” and collectively, the “Selling Stockholders”) of up to an aggregate of 16,894,212 shares of Matinas Biopharma Holdings, Inc. (the “Company”) common stock, par value $0.0001 per share (“Common Stock”) consisting of (i) 5,631,404 shares of Common Stock issuable upon the conversion of 3,300 shares of the Company’s Series C Convertible Preferred Stock, par value $0.0001 (the “Preferred Stock”), and (ii) 11,262,808 shares of Common Stock (the “Warrant Shares”) issuable upon the exercise of warrants (the “Warrants”).

This prospectus supplement is being filed solely to update the list of Selling Stockholders to reflect (x) the distribution of (i) 1,406 shares of Preferred Stock and (ii) Warrants to purchase 4,798,636 Warrant Shares, previously issued to Sanitam Partners LLC (“Sanitam”), to its underlying equityholders and (y) the assignment of (i) 265 shares of Preferred Stock and (ii) Warrants to purchase 800,000 Warrant Shares, previously issued to Platinum Point Capital LLC (“Platinum Point”), to SternAegis Ventures LLC Defined Benefit Plan for the Benefit of Adam K. Stern. No additional securities are being registered by this prospectus supplement. The shares covered hereby were previously registered for resale pursuant to the registration statement referenced herein.

You should read this prospectus supplement, together with the related prospectus and the additional information described under the headings “Incorporation of Certain Information by Reference” and “Where You Can Find More Information” carefully before you invest in any of our securities.

Our Common Stock is listed on the NYSE American LLC (“NYSE American”) under the symbol “MTNB.” On June 18, 2026, the last reported sale price of our Common Stock as reported on NYSE American was $0.60.

An investment in our securities involves a high degree of risk. Before deciding whether to invest in our securities, you should consider carefully the risks and uncertainties described in the section captioned “Risk Factors” contained in our Annual Report on Form 10-K for the fiscal year ended December 31, 2025, filed with the Securities and Exchange Commission, or the SEC, on March 31, 2026 and our other filings we make with the SEC from time to time, which are incorporated by reference herein in their entirety, together with other information in this prospectus and the information incorporated by reference herein.

Neither the Securities and Exchange Commission nor any state securities commission has approved or disapproved of these securities or determined if this prospectus is truthful or complete. Any representation to the contrary is a criminal offense.

The date of this prospectus supplement is June 22, 2026.

The following information is provided to update the Selling Stockholders table in the prospectus to reflect (i) the distribution of shares of Preferred Stock and Warrants, previously issued to Sanitam, to its underlying investors and (ii) the assignment of shares of Preferred Stock and Warrants, previously issued to Platinum Point, to SternAegis Ventures LLC Defined Benefit Plan for the Benefit of Adam K. Stern. This prospectus supplement includes information with respect to Selling Stockholders not previously listed in the prospectus.

SELLING STOCKHOLDERS

The following information is provided as of June 22, 2026 to update the “Selling Shareholders” section of the Prospectus to reflect (i) the pro rata distribution by Sanitam to its equityholders of 1,406 shares of Preferred Stock and Warrants to Purchase 4,798,636 Warrant Shares, and (ii) the assignment by Platinum Point to SternAegis Ventures LLC Defined Benefit Plan for the Benefit of Adam K. Stern of 265 shares of Preferred Stock and Warrants to Purchase 800,000 Warrant Shares, as reflected in the table set forth below.

With respect to only the Selling Shareholders listed in the table below, the information set forth in the table below supersedes and replaces the information regarding such Selling Shareholders in the Prospectus. Information regarding each of the Selling Shareholders listed in the table below is based on information provided by each of them as of the date of this prospectus supplement.

Information about the Selling Shareholders, including those listed below, may change over time. Since the date of the Prospectus, certain of the Selling Shareholders listed below have sold a portion of their shares of Common Stock registered thereunder and certain other Selling Shareholders may also have sold or otherwise transferred their shares of Common Stock registered thereunder. This prospectus supplement does not provide any updates with respect to any Selling Shareholders not listed in the table below.

* Less than 1 percent