<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019e1cd3-f910-7482-9a59-77225cdf10d8,g:ebfd5b70-e45d-4d37-9cb3-0c34f06d1c36-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="https://xbrl.org/2023/arcrole/summation-item" xlink:type="simple" xlink:href="https://www.xbrl.org/2023/calculation-1.1.xsd#summation-item"/>
  <link:roleRef roleURI="http://www.csx.com/role/STATEMENTSOFNETASSETSAVAILABLEFORBENEFITS" xlink:type="simple" xlink:href="csx-20260622.xsd#STATEMENTSOFNETASSETSAVAILABLEFORBENEFITS"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/STATEMENTSOFNETASSETSAVAILABLEFORBENEFITS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanLiability_019e1cd3-f911-76aa-889c-da41ef3b7929" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAccruedExpense_019e1cd3-f911-7906-9fa9-74e5bd267421" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanAccruedExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanLiability_019e1cd3-f911-76aa-889c-da41ef3b7929" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAccruedExpense_019e1cd3-f911-7906-9fa9-74e5bd267421" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7452-8a7c-7f0200d8dadf" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanAsset"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentPlanInterestInMasterTrustFairAndContractValue_019e1cd3-f911-7134-91a0-63ec980d8fcc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentPlanInterestInMasterTrustFairAndContractValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7452-8a7c-7f0200d8dadf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentPlanInterestInMasterTrustFairAndContractValue_019e1cd3-f911-7134-91a0-63ec980d8fcc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantContributionReceivable_019e1cd3-f911-72fa-932c-677c5d47a1f1" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanParticipantContributionReceivable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7452-8a7c-7f0200d8dadf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanParticipantContributionReceivable_019e1cd3-f911-72fa-932c-677c5d47a1f1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionReceivable_019e1cd3-f911-76a6-a64d-d0c83ef4bcce" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanEmployerContributionReceivable"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7452-8a7c-7f0200d8dadf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanEmployerContributionReceivable_019e1cd3-f911-76a6-a64d-d0c83ef4bcce" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant_019e1cd3-f911-7111-98ff-e721924b2838" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7452-8a7c-7f0200d8dadf" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanNoteReceivableFromParticipant_019e1cd3-f911-7111-98ff-e721924b2838" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019e1cd3-f911-766d-b282-b1432b28a555" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-793f-a045-4ffe23abb23d" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanAsset"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019e1cd3-f911-766d-b282-b1432b28a555" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-793f-a045-4ffe23abb23d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanLiability_019e1cd3-f911-7da5-9683-bd02cf178ee5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanLiability"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanNetAssetAvailableForBenefit_019e1cd3-f911-766d-b282-b1432b28a555" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanLiability_019e1cd3-f911-7da5-9683-bd02cf178ee5" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/STATEMENTOFCHANGESINNETASSETSAVAILABLEFORBENEFITS" xlink:type="simple" xlink:href="csx-20260622.xsd#STATEMENTOFCHANGESINNETASSETSAVAILABLEFORBENEFITS"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/STATEMENTOFCHANGESINNETASSETSAVAILABLEFORBENEFITS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019e1cd3-f911-7591-a272-bd9327a582f3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7890-9414-1e03ce2958f5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019e1cd3-f911-7591-a272-bd9327a582f3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7890-9414-1e03ce2958f5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019e1cd3-f911-70b3-bac4-cfea12c16595" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecrease_019e1cd3-f911-7591-a272-bd9327a582f3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019e1cd3-f911-70b3-bac4-cfea12c16595" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019e1cd3-f911-7775-ae66-74ee33a651d2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019e1cd3-f911-7ba0-a0b8-76f1fbb3192e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019e1cd3-f911-7775-ae66-74ee33a651d2" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForPaymentToParticipant_019e1cd3-f911-7ba0-a0b8-76f1fbb3192e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_EBPChangeInNetAssetAvailableForBenefitTransferFromPlan_019e1cd3-f911-76ce-8066-8a4c8e48c63d" xlink:href="csx-20260622.xsd#csx_EBPChangeInNetAssetAvailableForBenefitTransferFromPlan"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019e1cd3-f911-7775-ae66-74ee33a651d2" xlink:to="loc_csx_EBPChangeInNetAssetAvailableForBenefitTransferFromPlan_019e1cd3-f911-76ce-8066-8a4c8e48c63d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense_019e1cd3-f911-75ab-8da0-0f0cfc65239e" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecrease_019e1cd3-f911-7775-ae66-74ee33a651d2" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitDecreaseForAdministrativeExpense_019e1cd3-f911-75ab-8da0-0f0cfc65239e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7f54-b84d-f2f9af404fc3" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019e1cd3-f911-7d1a-8dd7-9ae3d17e1bbc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7f54-b84d-f2f9af404fc3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019e1cd3-f911-7d1a-8dd7-9ae3d17e1bbc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover_019e1cd3-f911-7ce3-ab6c-5034033b4ffc" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7f54-b84d-f2f9af404fc3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForParticipantContributionExcludingRollover_019e1cd3-f911-7ce3-ab6c-5034033b4ffc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019e1cd3-f911-711f-a6d3-e73db594247b" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7f54-b84d-f2f9af404fc3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForEmployerContributionCash_019e1cd3-f911-711f-a6d3-e73db594247b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_csx_EBPChangeInNetAssetAvailableForBenefitTransferToPlan_019e1cd3-f911-71fc-9bf1-316d2c1ec038" xlink:href="csx-20260622.xsd#csx_EBPChangeInNetAssetAvailableForBenefitTransferToPlan"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7f54-b84d-f2f9af404fc3" xlink:to="loc_csx_EBPChangeInNetAssetAvailableForBenefitTransferToPlan_019e1cd3-f911-71fc-9bf1-316d2c1ec038" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant_019e1cd3-f911-7532-9d54-ea0871faacc2" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncrease_019e1cd3-f911-7f54-b84d-f2f9af404fc3" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestIncomeOnNoteReceivableFromParticipant_019e1cd3-f911-7532-9d54-ea0871faacc2" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/InvestmentinMasterTrustSummarizedFinancialInformationDetails" xlink:type="simple" xlink:href="csx-20260622.xsd#InvestmentinMasterTrustSummarizedFinancialInformationDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/InvestmentinMasterTrustSummarizedFinancialInformationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7b50-bd70-6ef2cbf287d0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanAsset"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable_019e1cd3-f911-7f55-8b84-e040136a8009" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7b50-bd70-6ef2cbf287d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentIncomeReceivable_019e1cd3-f911-7f55-8b84-e040136a8009" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanReceivableInvestmentSale_019e1cd3-f911-75a6-a904-725f8100d3c8" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanReceivableInvestmentSale"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7b50-bd70-6ef2cbf287d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanReceivableInvestmentSale_019e1cd3-f911-75a6-a904-725f8100d3c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValue_019e1cd3-f911-7fad-91df-a2c6340169ad" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValue"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7b50-bd70-6ef2cbf287d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentFairValue_019e1cd3-f911-7fad-91df-a2c6340169ad" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentContractValue_019e1cd3-f911-783c-9df8-5007c2bbadb7" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanInvestmentContractValue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanAsset_019e1cd3-f911-7b50-bd70-6ef2cbf287d0" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanInvestmentContractValue_019e1cd3-f911-783c-9df8-5007c2bbadb7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanLiability_019e1cd3-f911-7427-9621-1e2df3bda3f5" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanLiability"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanLiabilityInvestmentPurchase_019e1cd3-f911-7429-bc70-1c86b504c887" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanLiabilityInvestmentPurchase"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanLiability_019e1cd3-f911-7427-9621-1e2df3bda3f5" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanLiabilityInvestmentPurchase_019e1cd3-f911-7429-bc70-1c86b504c887" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://www.csx.com/role/InvestmentinMasterTrustScheduleofInvestmentGainforMasterTrustDetails" xlink:type="simple" xlink:href="csx-20260622.xsd#InvestmentinMasterTrustScheduleofInvestmentGainforMasterTrustDetails"/>
  <link:calculationLink xlink:role="http://www.csx.com/role/InvestmentinMasterTrustScheduleofInvestmentGainforMasterTrustDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019e1cd3-f911-753c-a0ff-31f05bf33aee" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment_019e1cd3-f911-77e8-ad62-4427574532ab" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019e1cd3-f911-753c-a0ff-31f05bf33aee" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForGainLossOnInvestment_019e1cd3-f911-77e8-ad62-4427574532ab" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment_019e1cd3-f911-7b6e-ad4f-b284de66e4f0" xlink:href="https://xbrl.fasb.org/us-gaap/2026/ebp/elts/us-gaap-ebp-2026.xsd#us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseDecreaseForIncomeLossOnInvestment_019e1cd3-f911-753c-a0ff-31f05bf33aee" xlink:to="loc_us-gaap-ebp_EmployeeBenefitPlanChangeInNetAssetAvailableForBenefitIncreaseForInterestAndDividendIncomeOnInvestment_019e1cd3-f911-7b6e-ad4f-b284de66e4f0" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
