0001558569 1 2026-06-01 2026-06-01 0001558569 2026-06-01 2026-06-01 iso4217:USD pure shares S-3 EX-FILING FEES 0001558569 N/A N/A S-3 iSpecimen Inc. false Equity Common Stock, $0.0001 par value per share true 488290 3.2150 1569852.35 0.0001381 216.80 1569852.35 216.80 0.00 216.80 Pursuant to Rule 416 under the Securities Act, the securities being registered hereunder include such indeterminate number of additional shares of common stock as may be issued after the date hereof as a result of stock splits, stock dividends or similar transactions. Estimated solely for the purpose of calculating the registration fee in accordance with Rule 457(c) of the Securities Act of 1933, as amended, based upon the average of the high and low prices for a share of the registrant’s common stock as reported on the Nasdaq Capital Market on May 29, 2026. Consists of (i) up to 85,202 shares of common stock and (ii) up to 403,088 shares of common stock issuable upon exercise of pre-funded warrants, all of which were issued in connection with a private placement transaction completed on May 11, 2026, pursuant to a securities purchase agreement, dated as of May 8, 2026, and which shares are offered for resale by the selling stockholders named in the prospectus.