<?xml version="1.0" encoding="US-ASCII" standalone="no"?>
    <!-- Field: Doc-Info; Name: Generator; Value: GoFiler Complete; Version: 6.5a -->
    <!-- Field: Doc-Info; Name: VendorURI; Value: https://www.novaworks.com -->
    <!-- Field: Doc-Info; Name: Status; Value: 0x00000000 -->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrli="http://www.xbrl.org/2003/instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#Cover" roleURI="http://btcs.com/role/Cover" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#BalanceSheets" roleURI="http://btcs.com/role/BalanceSheets" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#BalanceSheetsParenthetical" roleURI="http://btcs.com/role/BalanceSheetsParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StatementsOfOperations" roleURI="http://btcs.com/role/StatementsOfOperations" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StatementsOfChangesInStockholdersEquity" roleURI="http://btcs.com/role/StatementsOfChangesInStockholdersEquity" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StatementsOfChangesInStockholdersEquityParenthetical" roleURI="http://btcs.com/role/StatementsOfChangesInStockholdersEquityParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StatementsOfCashFlows" roleURI="http://btcs.com/role/StatementsOfCashFlows" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#BusinessOrganizationAndNatureOfOperations" roleURI="http://btcs.com/role/BusinessOrganizationAndNatureOfOperations" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#BasisOfPresentation" roleURI="http://btcs.com/role/BasisOfPresentation" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfSignificantAccountingPolicies" roleURI="http://btcs.com/role/SummaryOfSignificantAccountingPolicies" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DigitalAssetsFairValue" roleURI="http://btcs.com/role/DigitalAssetsFairValue" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DigitalAssetsLiquidityPoolPositionsAndOtherIntangibleDigitalAssets" roleURI="http://btcs.com/role/DigitalAssetsLiquidityPoolPositionsAndOtherIntangibleDigitalAssets" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#FairValueMeasurements" roleURI="http://btcs.com/role/FairValueMeasurements" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#RevenuesAndCostOfRevenues" roleURI="http://btcs.com/role/RevenuesAndCostOfRevenues" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SegmentReporting" roleURI="http://btcs.com/role/SegmentReporting" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StockholdersEquity" roleURI="http://btcs.com/role/StockholdersEquity" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#Debt" roleURI="http://btcs.com/role/Debt" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DividendsAndCapitalDistributions" roleURI="http://btcs.com/role/DividendsAndCapitalDistributions" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#EmployeeBenefitPlans" roleURI="http://btcs.com/role/EmployeeBenefitPlans" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#Liquidity" roleURI="http://btcs.com/role/Liquidity" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#RelatedPartyTransactions" roleURI="http://btcs.com/role/RelatedPartyTransactions" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SubsequentEvents" roleURI="http://btcs.com/role/SubsequentEvents" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfSignificantAccountingPoliciesPolicies" roleURI="http://btcs.com/role/SummaryOfSignificantAccountingPoliciesPolicies" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfSignificantAccountingPoliciesTables" roleURI="http://btcs.com/role/SummaryOfSignificantAccountingPoliciesTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DigitalAssetsFairValueTables" roleURI="http://btcs.com/role/DigitalAssetsFairValueTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DigitalAssetsLiquidityPoolPositionsAndOtherIntangibleDigitalAssetsTables" roleURI="http://btcs.com/role/DigitalAssetsLiquidityPoolPositionsAndOtherIntangibleDigitalAssetsTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#FairValueMeasurementsTables" roleURI="http://btcs.com/role/FairValueMeasurementsTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#RevenuesAndCostOfRevenuesTables" roleURI="http://btcs.com/role/RevenuesAndCostOfRevenuesTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SegmentReportingTables" roleURI="http://btcs.com/role/SegmentReportingTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StockholdersEquityTables" roleURI="http://btcs.com/role/StockholdersEquityTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DebtTables" roleURI="http://btcs.com/role/DebtTables" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfEarningsPerShareAnti-dilutedDetails" roleURI="http://btcs.com/role/ScheduleOfEarningsPerShareAnti-dilutedDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfSignificantAccountingPoliciesDetailsNarrative" roleURI="http://btcs.com/role/SummaryOfSignificantAccountingPoliciesDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfCryptoAssetsHeldDetails" roleURI="http://btcs.com/role/ScheduleOfCryptoAssetsHeldDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfCryptoAssetsHeldDetailsParenthetical" roleURI="http://btcs.com/role/ScheduleOfCryptoAssetsHeldDetailsParenthetical" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfDigitalAssetsByOperationalUseDetails" roleURI="http://btcs.com/role/ScheduleOfDigitalAssetsByOperationalUseDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfCryptoAssetsRollforwardActivityDetails" roleURI="http://btcs.com/role/ScheduleOfCryptoAssetsRollforwardActivityDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfCarryingValueOfDigitalAssetsDetails" roleURI="http://btcs.com/role/ScheduleOfCarryingValueOfDigitalAssetsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfDigitalAssetsAccountedForAsIndefinite-livedIntangibleAssetsDetails" roleURI="http://btcs.com/role/ScheduleOfDigitalAssetsAccountedForAsIndefinite-livedIntangibleAssetsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfFairValueOfAssetsAndLiabilitiesValuedOnRecurringBasisDetails" roleURI="http://btcs.com/role/ScheduleOfFairValueOfAssetsAndLiabilitiesValuedOnRecurringBasisDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#FairValueMeasurementsDetailsNarrative" roleURI="http://btcs.com/role/FairValueMeasurementsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfChangesInFairValueAndOtherAdjustmentsOfWarrantsDetails" roleURI="http://btcs.com/role/ScheduleOfChangesInFairValueAndOtherAdjustmentsOfWarrantsDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfDisaggregationOfRevenuesDetails" roleURI="http://btcs.com/role/ScheduleOfDisaggregationOfRevenuesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfDisaggregationOfCostOfRevenuesDetails" roleURI="http://btcs.com/role/ScheduleOfDisaggregationOfCostOfRevenuesDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfSegmentRevenueAndGrossProfitLossDetails" roleURI="http://btcs.com/role/ScheduleOfSegmentRevenueAndGrossProfitLossDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SegmentReportingDetailsNarrative" roleURI="http://btcs.com/role/SegmentReportingDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#StockholdersEquityDetailsNarrative" roleURI="http://btcs.com/role/StockholdersEquityDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfWarrantActivityDetails" roleURI="http://btcs.com/role/SummaryOfWarrantActivityDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfRsuActivityRollforwardDetails" roleURI="http://btcs.com/role/SummaryOfRsuActivityRollforwardDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfVestingTriggerDetails" roleURI="http://btcs.com/role/ScheduleOfVestingTriggerDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfWeighted-averageAssumptionsUsedToEstimateFairValueDetails" roleURI="http://btcs.com/role/ScheduleOfWeighted-averageAssumptionsUsedToEstimateFairValueDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfOptionActivityDetails" roleURI="http://btcs.com/role/SummaryOfOptionActivityDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#ScheduleOfStock-basedCompensationExpenseDetails" roleURI="http://btcs.com/role/ScheduleOfStock-basedCompensationExpenseDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#SummaryOfDefiProtocolLendingActivityDetails" roleURI="http://btcs.com/role/SummaryOfDefiProtocolLendingActivityDetails" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DebtDetailsNarrative" roleURI="http://btcs.com/role/DebtDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#DividendsAndCapitalDistributionsDetailsNarrative" roleURI="http://btcs.com/role/DividendsAndCapitalDistributionsDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#EmployeeBenefitPlansDetailsNarrative" roleURI="http://btcs.com/role/EmployeeBenefitPlansDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#LiquidityDetailsNarrative" roleURI="http://btcs.com/role/LiquidityDetailsNarrative" />
    <link:roleRef xlink:type="simple" xlink:href="btcs-20260331.xsd#RelatedPartyTransactionsDetailsNarrative" roleURI="http://btcs.com/role/RelatedPartyTransactionsDetailsNarrative" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/Cover" xlink:title="00000001 - Document - Cover" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/BalanceSheets" xlink:title="00000002 - Statement - Condensed Balance Sheets">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AssetsCurrent" xlink:label="loc_us-gaapAssetsCurrent" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue" xlink:label="loc_us-gaapCashAndCashEquivalentsAtCarryingValue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapCashAndCashEquivalentsAtCarryingValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_StableCoins" xlink:label="loc_BTCSStableCoins" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_BTCSStableCoins" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CryptoAssetFairValueCurrent" xlink:label="loc_us-gaapCryptoAssetFairValueCurrent" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapCryptoAssetFairValueCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DigitalAssetsDefi" xlink:label="loc_BTCSDigitalAssetsDefi" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_BTCSDigitalAssetsDefi" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_StakedDigitalAssetsCurrencies" xlink:label="loc_BTCSStakedDigitalAssetsCurrencies" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_BTCSStakedDigitalAssetsCurrencies" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DigitalAssetsLiquidityPoolPositions" xlink:label="loc_BTCSDigitalAssetsLiquidityPoolPositions" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_BTCSDigitalAssetsLiquidityPoolPositions" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DigitalAssetsNonfungibleTokens" xlink:label="loc_BTCSDigitalAssetsNonfungibleTokens" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_BTCSDigitalAssetsNonfungibleTokens" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PrepaidExpenseCurrent" xlink:label="loc_us-gaapPrepaidExpenseCurrent" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssetsCurrent" xlink:to="loc_us-gaapPrepaidExpenseCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Assets" xlink:label="loc_us-gaapAssets" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapAssetsCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Investments" xlink:label="loc_us-gaapInvestments" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapInvestments" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PropertyPlantAndEquipmentNet" xlink:label="loc_us-gaapPropertyPlantAndEquipmentNet" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapAssets" xlink:to="loc_us-gaapPropertyPlantAndEquipmentNet" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesCurrent" xlink:label="loc_us-gaapLiabilitiesCurrent" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccountsPayableAndAccruedLiabilitiesCurrent" xlink:label="loc_us-gaapAccountsPayableAndAccruedLiabilitiesCurrent" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapAccountsPayableAndAccruedLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AccruedSalariesCurrent" xlink:label="loc_us-gaapAccruedSalariesCurrent" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapAccruedSalariesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestPayableCurrent" xlink:label="loc_us-gaapInterestPayableCurrent" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapInterestPayableCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LoansPayableCurrent" xlink:label="loc_us-gaapLoansPayableCurrent" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapLoansPayableCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_DerivativeLiabilitiesCurrent" xlink:label="loc_us-gaapDerivativeLiabilitiesCurrent" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesCurrent" xlink:to="loc_us-gaapDerivativeLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Liabilities" xlink:label="loc_us-gaapLiabilities" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapLiabilitiesCurrent" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ConvertibleLongTermNotesPayable" xlink:label="loc_us-gaapConvertibleLongTermNotesPayable" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilities" xlink:to="loc_us-gaapConvertibleLongTermNotesPayable" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_StockholdersEquity" xlink:label="loc_us-gaapStockholdersEquity" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PreferredStockValue" xlink:label="loc_us-gaapPreferredStockValue" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapPreferredStockValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommonStockValue" xlink:label="loc_us-gaapCommonStockValue" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapCommonStockValue" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AdditionalPaidInCapital" xlink:label="loc_us-gaapAdditionalPaidInCapital" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapAdditionalPaidInCapital" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RetainedEarningsAccumulatedDeficit" xlink:label="loc_us-gaapRetainedEarningsAccumulatedDeficit" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapStockholdersEquity" xlink:to="loc_us-gaapRetainedEarningsAccumulatedDeficit" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_LiabilitiesAndStockholdersEquity" xlink:label="loc_us-gaapLiabilitiesAndStockholdersEquity" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapLiabilities" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CommitmentsAndContingencies" xlink:label="loc_us-gaapCommitmentsAndContingencies" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapCommitmentsAndContingencies" xlink:type="arc" weight="1" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapLiabilitiesAndStockholdersEquity" xlink:to="loc_us-gaapStockholdersEquity" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/BalanceSheetsParenthetical" xlink:title="00000003 - Statement - Condensed Balance Sheets (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StatementsOfOperations" xlink:title="00000004 - Statement - Condensed Statements of Operations (Unaudited)">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GrossProfit" xlink:label="loc_us-gaapGrossProfit" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax" xlink:label="loc_us-gaapRevenueFromContractWithCustomerExcludingAssessedTax" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapGrossProfit" xlink:to="loc_us-gaapRevenueFromContractWithCustomerExcludingAssessedTax" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CostOfRevenue" xlink:label="loc_us-gaapCostOfRevenue" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapGrossProfit" xlink:to="loc_us-gaapCostOfRevenue" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_OperatingExpenses" xlink:label="loc_us-gaapOperatingExpenses" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProfessionalFees" xlink:label="loc_us-gaapProfessionalFees" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapProfessionalFees" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_GeneralAndAdministrativeExpense" xlink:label="loc_us-gaapGeneralAndAdministrativeExpense" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapGeneralAndAdministrativeExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ResearchAndDevelopmentExpense" xlink:label="loc_us-gaapResearchAndDevelopmentExpense" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapResearchAndDevelopmentExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CompensationExpenseExcludingCostOfGoodAndServiceSold" xlink:label="loc_us-gaapCompensationExpenseExcludingCostOfGoodAndServiceSold" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapCompensationExpenseExcludingCostOfGoodAndServiceSold" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_MarketingExpense" xlink:label="loc_us-gaapMarketingExpense" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_us-gaapMarketingExpense" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_ImpairmentLossOnIntangibleDigitalAssets" xlink:label="loc_BTCSImpairmentLossOnIntangibleDigitalAssets" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_BTCSImpairmentLossOnIntangibleDigitalAssets" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_RealizedGainsLossesOnDigitalAssetCurrencyTransactions" xlink:label="loc_BTCSRealizedGainsLossesOnDigitalAssetCurrencyTransactions" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_BTCSRealizedGainsLossesOnDigitalAssetCurrencyTransactions" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_UnrealizedGainLossOnDigitalAssets" xlink:label="loc_BTCSUnrealizedGainLossOnDigitalAssets" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapOperatingExpenses" xlink:to="loc_BTCSUnrealizedGainLossOnDigitalAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NonoperatingIncomeExpense" xlink:label="loc_us-gaapNonoperatingIncomeExpense" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_InterestExpenseNonoperating" xlink:label="loc_us-gaapInterestExpenseNonoperating" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNonoperatingIncomeExpense" xlink:to="loc_us-gaapInterestExpenseNonoperating" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAdjustmentOfWarrants" xlink:label="loc_us-gaapFairValueAdjustmentOfWarrants" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNonoperatingIncomeExpense" xlink:to="loc_us-gaapFairValueAdjustmentOfWarrants" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaapNetIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapGrossProfit" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapOperatingExpenses" xlink:type="arc" weight="-1" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetIncomeLoss" xlink:to="loc_us-gaapNonoperatingIncomeExpense" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StatementsOfChangesInStockholdersEquity" xlink:title="00000005 - Statement - Condensed Statements of Changes in Stockholders&apos; Equity (Unaudited)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StatementsOfChangesInStockholdersEquityParenthetical" xlink:title="00000006 - Statement - Condensed Statements of Changes in Stockholders&apos; Equity (Unaudited) (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StatementsOfCashFlows" xlink:title="00000007 - Statement - Condensed Statements of Cash Flows (Unaudited)">
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetIncomeLoss" xlink:label="loc_us-gaapNetIncomeLoss" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapNetIncomeLoss" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_Depreciation" xlink:label="loc_us-gaapDepreciation" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapDepreciation" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ShareBasedCompensation" xlink:label="loc_us-gaapShareBasedCompensation" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapShareBasedCompensation" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_BlockchainInfrastructureRevenue" xlink:label="loc_BTCSBlockchainInfrastructureRevenue" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSBlockchainInfrastructureRevenue" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DefiRevenue" xlink:label="loc_BTCSDefiRevenue" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSDefiRevenue" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_BlockchainbasedPaymentsSettledInDigitalAssets" xlink:label="loc_BTCSBlockchainbasedPaymentsSettledInDigitalAssets" />
      <link:calculationArc order="6" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSBlockchainbasedPaymentsSettledInDigitalAssets" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DefiInterestExpenseSettledInDigitalAssets" xlink:label="loc_BTCSDefiInterestExpenseSettledInDigitalAssets" />
      <link:calculationArc order="7" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSDefiInterestExpenseSettledInDigitalAssets" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_BlockchainNetworkFees" xlink:label="loc_BTCSBlockchainNetworkFees" />
      <link:calculationArc order="8" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSBlockchainNetworkFees" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_FairValueAdjustmentOfWarrants" xlink:label="loc_us-gaapFairValueAdjustmentOfWarrants" />
      <link:calculationArc order="9" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapFairValueAdjustmentOfWarrants" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts" xlink:label="loc_us-gaapAmortizationOfFinancingCostsAndDiscounts" />
      <link:calculationArc order="10" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapAmortizationOfFinancingCostsAndDiscounts" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_RealizedGainsLossesOnDigitalAssetCurrencyTransactions" xlink:label="loc_BTCSRealizedGainsLossesOnDigitalAssetCurrencyTransactions" />
      <link:calculationArc order="11" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSRealizedGainsLossesOnDigitalAssetCurrencyTransactions" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_UnrealizedGainLossOnDigitalAssets" xlink:label="loc_BTCSUnrealizedGainLossOnDigitalAssets" />
      <link:calculationArc order="12" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSUnrealizedGainLossOnDigitalAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_ImpairmentLossesOnIntangibleDigitalAssets" xlink:label="loc_BTCSImpairmentLossesOnIntangibleDigitalAssets" />
      <link:calculationArc order="13" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSImpairmentLossesOnIntangibleDigitalAssets" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_IncreaseDecreaseInStableCoins" xlink:label="loc_BTCSIncreaseDecreaseInStableCoins" />
      <link:calculationArc order="14" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSIncreaseDecreaseInStableCoins" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_IncreaseDecreaseInIntangibleDigitalAssets" xlink:label="loc_BTCSIncreaseDecreaseInIntangibleDigitalAssets" />
      <link:calculationArc order="15" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_BTCSIncreaseDecreaseInIntangibleDigitalAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:label="loc_us-gaapIncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" />
      <link:calculationArc order="16" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInAccountsPayableAndAccruedLiabilities" xlink:label="loc_us-gaapIncreaseDecreaseInAccountsPayableAndAccruedLiabilities" />
      <link:calculationArc order="17" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInAccountsPayableAndAccruedLiabilities" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInAccruedSalaries" xlink:label="loc_us-gaapIncreaseDecreaseInAccruedSalaries" />
      <link:calculationArc order="18" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInAccruedSalaries" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_IncreaseDecreaseInAccruedInterestReceivableNet" xlink:label="loc_us-gaapIncreaseDecreaseInAccruedInterestReceivableNet" />
      <link:calculationArc order="19" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:to="loc_us-gaapIncreaseDecreaseInAccruedInterestReceivableNet" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_PurchaseOfProductiveDigitalAssetsCurrenciesForValidating" xlink:label="loc_BTCSPurchaseOfProductiveDigitalAssetsCurrenciesForValidating" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_BTCSPurchaseOfProductiveDigitalAssetsCurrenciesForValidating" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_SaleOfProductiveDigitalAssetsCurrencies" xlink:label="loc_BTCSSaleOfProductiveDigitalAssetsCurrencies" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_BTCSSaleOfProductiveDigitalAssetsCurrencies" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquireInvestments" xlink:label="loc_us-gaapPaymentsToAcquireInvestments" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsToAcquireInvestments" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment" xlink:label="loc_us-gaapPaymentsToAcquirePropertyPlantAndEquipment" />
      <link:calculationArc order="4" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapPaymentsToAcquirePropertyPlantAndEquipment" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromSaleOfPropertyPlantAndEquipment" xlink:label="loc_us-gaapProceedsFromSaleOfPropertyPlantAndEquipment" />
      <link:calculationArc order="5" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:to="loc_us-gaapProceedsFromSaleOfPropertyPlantAndEquipment" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities" xlink:label="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfCommonStock" xlink:label="loc_us-gaapProceedsFromIssuanceOfCommonStock" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapProceedsFromIssuanceOfCommonStock" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_ProceedsFromIssuanceOfDebt" xlink:label="loc_us-gaapProceedsFromIssuanceOfDebt" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapProceedsFromIssuanceOfDebt" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_RepaymentsOfConvertibleDebt" xlink:label="loc_us-gaapRepaymentsOfConvertibleDebt" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:to="loc_us-gaapRepaymentsOfConvertibleDebt" xlink:type="arc" weight="-1" />
      <link:loc xlink:type="locator" xlink:href="https://xbrl.fasb.org/us-gaap/2026/elts/us-gaap-2026.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:label="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapNetCashProvidedByUsedInOperatingActivities" xlink:type="arc" weight="1" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapNetCashProvidedByUsedInInvestingActivities" xlink:type="arc" weight="1" />
      <link:calculationArc order="3" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaapCashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect" xlink:to="loc_us-gaapNetCashProvidedByUsedInFinancingActivities" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/BusinessOrganizationAndNatureOfOperations" xlink:title="999013 - Disclosure - Business Organization and Nature of Operations" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/BasisOfPresentation" xlink:title="999014 - Disclosure - Basis of Presentation" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfSignificantAccountingPolicies" xlink:title="999015 - Disclosure - Summary of Significant Accounting Policies" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DigitalAssetsFairValue" xlink:title="999016 - Disclosure - Digital Assets (Fair Value)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DigitalAssetsLiquidityPoolPositionsAndOtherIntangibleDigitalAssets" xlink:title="999017 - Disclosure - Digital Assets (Liquidity Pool Positions and Other Intangible Digital Assets)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/FairValueMeasurements" xlink:title="999018 - Disclosure - Fair Value Measurements" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/RevenuesAndCostOfRevenues" xlink:title="999019 - Disclosure - Revenues and Cost of Revenues" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SegmentReporting" xlink:title="999020 - Disclosure - Segment Reporting" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StockholdersEquity" xlink:title="999021 - Disclosure - Stockholders&#8217; Equity" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/Debt" xlink:title="999022 - Disclosure - Debt" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DividendsAndCapitalDistributions" xlink:title="999023 - Disclosure - Dividends and Capital Distributions" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/EmployeeBenefitPlans" xlink:title="999024 - Disclosure - Employee Benefit Plans" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/Liquidity" xlink:title="999025 - Disclosure - Liquidity" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/RelatedPartyTransactions" xlink:title="999026 - Disclosure - Related Party Transactions" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SubsequentEvents" xlink:title="999027 - Disclosure - Subsequent Events" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfSignificantAccountingPoliciesPolicies" xlink:title="999028 - Disclosure - Summary of Significant Accounting Policies (Policies)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfSignificantAccountingPoliciesTables" xlink:title="999029 - Disclosure - Summary of Significant Accounting Policies (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DigitalAssetsFairValueTables" xlink:title="999030 - Disclosure - Digital Assets (Fair Value) (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DigitalAssetsLiquidityPoolPositionsAndOtherIntangibleDigitalAssetsTables" xlink:title="999031 - Disclosure - Digital Assets (Liquidity Pool Positions and Other Intangible Digital Assets) (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/FairValueMeasurementsTables" xlink:title="999032 - Disclosure - Fair Value Measurements (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/RevenuesAndCostOfRevenuesTables" xlink:title="999033 - Disclosure - Revenues and Cost of Revenues (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SegmentReportingTables" xlink:title="999034 - Disclosure - Segment Reporting (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StockholdersEquityTables" xlink:title="999035 - Disclosure - Stockholders&#8217; Equity (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DebtTables" xlink:title="999036 - Disclosure - Debt (Tables)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfEarningsPerShareAnti-dilutedDetails" xlink:title="999037 - Disclosure - Schedule of Earnings Per Share Anti-diluted (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfSignificantAccountingPoliciesDetailsNarrative" xlink:title="999038 - Disclosure - Summary of Significant Accounting Policies (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfCryptoAssetsHeldDetails" xlink:title="999039 - Disclosure - Schedule of Crypto Assets Held (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfCryptoAssetsHeldDetailsParenthetical" xlink:title="999040 - Disclosure - Schedule of Crypto Assets Held (Details) (Parenthetical)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfDigitalAssetsByOperationalUseDetails" xlink:title="999041 - Disclosure - Schedule of Digital Assets by Operational Use (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfCryptoAssetsRollforwardActivityDetails" xlink:title="999042 - Disclosure - Schedule of Crypto Assets Rollforward Activity (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfCarryingValueOfDigitalAssetsDetails" xlink:title="999043 - Disclosure - Schedule of Carrying Value of Digital Assets (Details)">
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DigitalAssetsCarryingValue" xlink:label="loc_BTCSDigitalAssetsCarryingValue" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DigitalAssetsLiquidityPoolPositions" xlink:label="loc_BTCSDigitalAssetsLiquidityPoolPositions" />
      <link:calculationArc order="1" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_BTCSDigitalAssetsCarryingValue" xlink:to="loc_BTCSDigitalAssetsLiquidityPoolPositions" xlink:type="arc" weight="1" />
      <link:loc xlink:type="locator" xlink:href="btcs-20260331.xsd#BTCS_DigitalAssetsNonfungibleTokens" xlink:label="loc_BTCSDigitalAssetsNonfungibleTokens" />
      <link:calculationArc order="2" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_BTCSDigitalAssetsCarryingValue" xlink:to="loc_BTCSDigitalAssetsNonfungibleTokens" xlink:type="arc" weight="1" />
    </link:calculationLink>
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfDigitalAssetsAccountedForAsIndefinite-livedIntangibleAssetsDetails" xlink:title="999044 - Disclosure - Schedule of Digital Assets Accounted for as Indefinite-lived Intangible Assets (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfFairValueOfAssetsAndLiabilitiesValuedOnRecurringBasisDetails" xlink:title="999045 - Disclosure - Schedule of Fair Value of Assets and Liabilities Valued on Recurring Basis (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/FairValueMeasurementsDetailsNarrative" xlink:title="999046 - Disclosure - Fair Value Measurements (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfChangesInFairValueAndOtherAdjustmentsOfWarrantsDetails" xlink:title="999047 - Disclosure - Schedule of Changes in Fair Value and Other Adjustments of Warrants (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfDisaggregationOfRevenuesDetails" xlink:title="999048 - Disclosure - Schedule of Disaggregation of Revenues (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfDisaggregationOfCostOfRevenuesDetails" xlink:title="999049 - Disclosure - Schedule of Disaggregation of Cost of Revenues (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfSegmentRevenueAndGrossProfitLossDetails" xlink:title="999050 - Disclosure - Schedule of Segment Revenue and Gross Profit (loss) (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SegmentReportingDetailsNarrative" xlink:title="999051 - Disclosure - Segment Reporting (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/StockholdersEquityDetailsNarrative" xlink:title="999052 - Disclosure - Stockholders&#8217; Equity (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfWarrantActivityDetails" xlink:title="999053 - Disclosure - Summary of Warrant Activity (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfRsuActivityRollforwardDetails" xlink:title="999054 - Disclosure - Summary of RSU Activity Rollforward (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfVestingTriggerDetails" xlink:title="999055 - Disclosure - Schedule of Vesting Trigger (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfWeighted-averageAssumptionsUsedToEstimateFairValueDetails" xlink:title="999056 - Disclosure - Schedule of Weighted-Average Assumptions Used to Estimate Fair Value (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfOptionActivityDetails" xlink:title="999057 - Disclosure - Summary of Option Activity (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/ScheduleOfStock-basedCompensationExpenseDetails" xlink:title="999058 - Disclosure - Schedule of Stock-based Compensation Expense (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/SummaryOfDefiProtocolLendingActivityDetails" xlink:title="999059 - Disclosure - Summary of Defi Protocol Lending Activity (Details)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DebtDetailsNarrative" xlink:title="999060 - Disclosure - Debt (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/DividendsAndCapitalDistributionsDetailsNarrative" xlink:title="999061 - Disclosure - Dividends and Capital Distributions (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/EmployeeBenefitPlansDetailsNarrative" xlink:title="999062 - Disclosure - Employee Benefit Plans (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/LiquidityDetailsNarrative" xlink:title="999063 - Disclosure - Liquidity (Details Narrative)" />
    <link:calculationLink xlink:type="extended" xlink:role="http://btcs.com/role/RelatedPartyTransactionsDetailsNarrative" xlink:title="999064 - Disclosure - Related Party Transactions (Details Narrative)" />
</link:linkbase>
