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        <link:definition xmlns="http://www.xbrl.org/2003/linkbase">995529 - Disclosure - Note 20 - Discontinued Operations - Schedule of Discontinued Operations (Details)</link:definition>
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  <xsd:element abstract="true" id="aytu_OtherWarrantsMember" name="OtherWarrantsMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_PaymentsOfFixedPaymentArrangements" name="PaymentsOfFixedPaymentArrangements" nillable="true" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType" xbrli:balance="credit" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_PolicyTable" name="PolicyTable" nillable="true" substitutionGroup="xbrldt:hypercubeItem" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_PreFundedWarrantsMember" name="PreFundedWarrantsMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_PrincipalOfficeDenverColoradoMember" name="PrincipalOfficeDenverColoradoMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_ProceedsFromIssuanceOrSaleOfEquityNet" name="ProceedsFromIssuanceOrSaleOfEquityNet" nillable="true" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType" xbrli:balance="debit" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_PublicOfferingMember" name="PublicOfferingMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_RXSegmentMember" name="RXSegmentMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_ReclassifiedFromDerivativeLiabilityToEquityMember" name="ReclassifiedFromDerivativeLiabilityToEquityMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_RestrictedStockAndRestrictedStockUnitsMember" name="RestrictedStockAndRestrictedStockUnitsMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_RestrictedStockAndRestrictedStockUnitsRSUsMember" name="RestrictedStockAndRestrictedStockUnitsRSUsMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_SaleOfStockMaximumAmountOfEquityIssuable" name="SaleOfStockMaximumAmountOfEquityIssuable" nillable="true" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType" xbrli:balance="credit" xbrli:periodType="instant"/>
  <xsd:element id="aytu_ScheduleOfLeaseByBalanceSheetInformationTableTextBlock" name="ScheduleOfLeaseByBalanceSheetInformationTableTextBlock" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_StockIssuedDuringPeriodSharesWarrantsExercised" name="StockIssuedDuringPeriodSharesWarrantsExercised" nillable="true" substitutionGroup="xbrli:item" type="xbrli:sharesItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_StockIssuedDuringPeriodValueWarrantsExercised" name="StockIssuedDuringPeriodValueWarrantsExercised" nillable="true" substitutionGroup="xbrli:item" type="xbrli:monetaryItemType" xbrli:balance="credit" xbrli:periodType="duration"/>
  <xsd:element id="aytu_SupplyAndDistributionCommitmentPeriod" name="SupplyAndDistributionCommitmentPeriod" nillable="true" substitutionGroup="xbrli:item" type="xbrli:durationItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_SupplyAndDistributionCommitmentRoyaltyPercentageOfNetSales" name="SupplyAndDistributionCommitmentRoyaltyPercentageOfNetSales" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:percentItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_TRRPProprietaryTechnologyMember" name="TRRPProprietaryTechnologyMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_The2015PlanMember" name="The2015PlanMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_The2020ShelfMember" name="The2020ShelfMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_The2021ShelfMember" name="The2021ShelfMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_The2023EquityIncentivePlanMember" name="The2023EquityIncentivePlanMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_ThePediatricPortfolioMember" name="ThePediatricPortfolioMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_TrisKarbinalAgreementMember" name="TrisKarbinalAgreementMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_WarrantsExcludingPrefundedWarrantsMember" name="WarrantsExcludingPrefundedWarrantsMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_WarrantsOrRightsDisclosureTextBlock" name="WarrantsOrRightsDisclosureTextBlock" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType" xbrli:periodType="duration"/>
  <xsd:element id="aytu_WarrantsPolicyTextBlock" name="WarrantsPolicyTextBlock" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:textBlockItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_WeightedaverageOfWarrantsExercisedToCommonStockMember" name="WeightedaverageOfWarrantsExercisedToCommonStockMember" nillable="true" substitutionGroup="xbrli:item" type="dtr-types:domainItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-11-debt-future-principal-payments-details" name="statement-statement-note-11-debt-future-principal-payments-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-11-debt-schedule-of-debt-details" name="statement-statement-note-11-debt-schedule-of-debt-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-11-debt-tables" name="statement-statement-note-11-debt-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-12-fair-value-measurements-recurring-fair-value-measurements-details" name="statement-statement-note-12-fair-value-measurements-recurring-fair-value-measurements-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-12-fair-value-measurements-summary-of-level-3-changes-details" name="statement-statement-note-12-fair-value-measurements-summary-of-level-3-changes-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-12-fair-value-measurements-tables" name="statement-statement-note-12-fair-value-measurements-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-12-fair-value-measurements-valuation-assumptions-details" name="statement-statement-note-12-fair-value-measurements-valuation-assumptions-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-15-equity-incentive-plan-tables" name="statement-statement-note-15-equity-incentive-plan-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-15-equity-incentive-plans-restricted-stock-activity-details" name="statement-statement-note-15-equity-incentive-plans-restricted-stock-activity-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-15-equity-incentive-plans-stock-compensation-expense-2-details" name="statement-statement-note-15-equity-incentive-plans-stock-compensation-expense-2-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-15-equity-incentive-plans-stock-compensation-expense-details" name="statement-statement-note-15-equity-incentive-plans-stock-compensation-expense-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-15-equity-incentive-plans-stock-option-activity-details" name="statement-statement-note-15-equity-incentive-plans-stock-option-activity-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-16-warrants-schedule-of-warrants-outstanding-details" name="statement-statement-note-16-warrants-schedule-of-warrants-outstanding-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-16-warrants-tables" name="statement-statement-note-16-warrants-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-17-earnings-per-share-schedule-of-antidilutive-securities-details" name="statement-statement-note-17-earnings-per-share-schedule-of-antidilutive-securities-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-17-earnings-per-share-schedule-of-earnings-per-share-details" name="statement-statement-note-17-earnings-per-share-schedule-of-earnings-per-share-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-17-earnings-per-share-tables" name="statement-statement-note-17-earnings-per-share-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-20-discontinued-operations-schedule-of-discontinued-operations-details" name="statement-statement-note-20-discontinued-operations-schedule-of-discontinued-operations-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-20-discontinued-operations-tables" name="statement-statement-note-20-discontinued-operations-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-3-revenue-disaggregation-of-revenue-details" name="statement-statement-note-3-revenue-disaggregation-of-revenue-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-3-revenue-tables" name="statement-statement-note-3-revenue-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-4-inventories-schedule-of-inventories-details" name="statement-statement-note-4-inventories-schedule-of-inventories-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-4-inventories-tables" name="statement-statement-note-4-inventories-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-5-property-and-equipment-schedule-of-property-and-equipment-details" name="statement-statement-note-5-property-and-equipment-schedule-of-property-and-equipment-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-5-property-and-equipment-tables" name="statement-statement-note-5-property-and-equipment-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-6-leases-lease-balance-sheet-information-details" name="statement-statement-note-6-leases-lease-balance-sheet-information-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-6-leases-lease-balance-sheet-information-details-parentheticals" name="statement-statement-note-6-leases-lease-balance-sheet-information-details-parentheticals" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-6-leases-lease-cost-details" name="statement-statement-note-6-leases-lease-cost-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-6-leases-schedule-of-lease-liability-maturity-details" name="statement-statement-note-6-leases-schedule-of-lease-liability-maturity-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-6-leases-tables" name="statement-statement-note-6-leases-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-7-intangible-assets-future-amortization-expense-details" name="statement-statement-note-7-intangible-assets-future-amortization-expense-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-7-intangible-assets-schedule-of-intangible-assets-details" name="statement-statement-note-7-intangible-assets-schedule-of-intangible-assets-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-7-intangible-assets-summary-of-amortization-expense-details" name="statement-statement-note-7-intangible-assets-summary-of-amortization-expense-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-7-intangible-assets-tables" name="statement-statement-note-7-intangible-assets-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-8-accrued-liabilities-schedule-of-accrued-liabilities-details" name="statement-statement-note-8-accrued-liabilities-schedule-of-accrued-liabilities-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-8-accrued-liabilities-tables" name="statement-statement-note-8-accrued-liabilities-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-9-other-liabilities-schedule-of-other-liabilities-details" name="statement-statement-note-9-other-liabilities-schedule-of-other-liabilities-details" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-note-9-other-liabilities-tables" name="statement-statement-note-9-other-liabilities-tables" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
  <xsd:element abstract="true" id="aytu_statement-statement-significant-accounting-policies-policies" name="statement-statement-significant-accounting-policies-policies" nillable="true" substitutionGroup="xbrli:item" type="xbrli:stringItemType" xbrli:periodType="duration"/>
</xsd:schema>
