<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2026 Workiva-->
<!--r:019d0bd2-916e-75d7-9290-411216ff74b9,g:6b22807f-c81c-4fdd-847f-cb61bfa0d194-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="https://xbrl.org/2023/arcrole/summation-item" xlink:type="simple" xlink:href="https://www.xbrl.org/2023/calculation-1.1.xsd#summation-item"/>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/CONDENSEDBALANCESHEETS" xlink:type="simple" xlink:href="dtil-20260331.xsd#CONDENSEDBALANCESHEETS"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/CONDENSEDBALANCESHEETS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_019d0bd2-921f-7b0a-8610-10aec1394db7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_AssetsCurrent_019d0bd2-921f-7b0a-8610-10aec1394db7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_019d0bd2-921f-77bf-a22c-f0d3af5afeac" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_019d0bd2-921f-77bf-a22c-f0d3af5afeac" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalents_019d0bd2-921f-797d-a1c1-594f581e22b5" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RestrictedCashAndCashEquivalents"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalents_019d0bd2-921f-797d-a1c1-594f581e22b5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_019d0bd2-921f-7097-92f5-6cdbe121a812" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IntangibleAssetsNetExcludingGoodwill"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_IntangibleAssetsNetExcludingGoodwill_019d0bd2-921f-7097-92f5-6cdbe121a812" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_019d0bd2-921f-75b5-9201-7300b9443cfb" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_019d0bd2-921f-75b5-9201-7300b9443cfb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFVNINoncurrent_019d0bd2-921f-78c6-8650-49b1518d2c13" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EquitySecuritiesFVNINoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_EquitySecuritiesFVNINoncurrent_019d0bd2-921f-78c6-8650-49b1518d2c13" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_019d0bd2-921f-7b48-9271-34fba45287c0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_019d0bd2-921f-773a-a418-f0a44ee3cb12" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_019d0bd2-921f-7b48-9271-34fba45287c0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7652-8e34-d24dbb40d223" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockValue_019d0bd2-921f-7de6-8ac3-1938e80b2dd4" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PreferredStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7652-8e34-d24dbb40d223" xlink:to="loc_us-gaap_PreferredStockValue_019d0bd2-921f-7de6-8ac3-1938e80b2dd4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_019d0bd2-921f-77f2-b07f-80d277626e77" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7652-8e34-d24dbb40d223" xlink:to="loc_us-gaap_CommonStockValue_019d0bd2-921f-77f2-b07f-80d277626e77" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalCommonStock_019d0bd2-921f-78f7-84d9-8469abf38496" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AdditionalPaidInCapitalCommonStock"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7652-8e34-d24dbb40d223" xlink:to="loc_us-gaap_AdditionalPaidInCapitalCommonStock_019d0bd2-921f-78f7-84d9-8469abf38496" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_019d0bd2-921f-7e75-8d95-2da857e3a036" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7652-8e34-d24dbb40d223" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_019d0bd2-921f-7e75-8d95-2da857e3a036" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockValue_019d0bd2-921f-754e-8959-a7f598619b9c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_TreasuryStockValue"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7652-8e34-d24dbb40d223" xlink:to="loc_us-gaap_TreasuryStockValue_019d0bd2-921f-754e-8959-a7f598619b9c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_019d0bd2-921f-7e49-82bb-13af2b962570" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_019d0bd2-921f-7d7d-a8bc-65bceb501056" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_019d0bd2-921f-7e49-82bb-13af2b962570" xlink:to="loc_us-gaap_Liabilities_019d0bd2-921f-7d7d-a8bc-65bceb501056" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7f54-942f-65ef8fd73fd0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_019d0bd2-921f-7e49-82bb-13af2b962570" xlink:to="loc_us-gaap_StockholdersEquity_019d0bd2-921f-7f54-942f-65ef8fd73fd0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_019d0bd2-921f-728d-b8fb-77417f5c29dc" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019d0bd2-921f-72bb-a54c-a1eb9562fbfd" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019d0bd2-921f-728d-b8fb-77417f5c29dc" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019d0bd2-921f-72bb-a54c-a1eb9562fbfd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableNetCurrent_019d0bd2-921f-746e-8e75-71b5e17458a5" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AccountsReceivableNetCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019d0bd2-921f-728d-b8fb-77417f5c29dc" xlink:to="loc_us-gaap_AccountsReceivableNetCurrent_019d0bd2-921f-746e-8e75-71b5e17458a5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MarketableSecuritiesCurrent_019d0bd2-921f-7662-88ae-341a78ffd186" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_MarketableSecuritiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019d0bd2-921f-728d-b8fb-77417f5c29dc" xlink:to="loc_us-gaap_MarketableSecuritiesCurrent_019d0bd2-921f-7662-88ae-341a78ffd186" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseCurrent_019d0bd2-921f-7a86-9cf4-b6a0e77fa092" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PrepaidExpenseCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019d0bd2-921f-728d-b8fb-77417f5c29dc" xlink:to="loc_us-gaap_PrepaidExpenseCurrent_019d0bd2-921f-7a86-9cf4-b6a0e77fa092" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsCurrent_019d0bd2-921f-75a4-9459-c11b8f0f2369" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherAssetsCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_019d0bd2-921f-728d-b8fb-77417f5c29dc" xlink:to="loc_us-gaap_OtherAssetsCurrent_019d0bd2-921f-75a4-9459-c11b8f0f2369" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7970-bf04-7b3a150659a1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:to="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7970-bf04-7b3a150659a1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansPayable_019d0bd2-921f-7c0a-baf9-20be9100eac0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LoansPayable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:to="loc_us-gaap_LoansPayable_019d0bd2-921f-7c0a-baf9-20be9100eac0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_019d0bd2-921f-7675-98a6-208d8cee4cb8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_019d0bd2-921f-7675-98a6-208d8cee4cb8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_WarrantLiabilityNoncurrent_019d0bd2-921f-7cfc-906f-2baf02df14fe" xlink:href="dtil-20260331.xsd#dtil_WarrantLiabilityNoncurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:to="loc_dtil_WarrantLiabilityNoncurrent_019d0bd2-921f-7cfc-906f-2baf02df14fe" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_019d0bd2-921f-765a-a893-b1c84d2c154e" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_019d0bd2-921f-765a-a893-b1c84d2c154e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_019d0bd2-921f-7689-a55e-5e4848a700b1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_BusinessCombinationContingentConsiderationLiability"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_019d0bd2-921f-71bd-940e-700bde79942d" xlink:to="loc_us-gaap_BusinessCombinationContingentConsiderationLiability_019d0bd2-921f-7689-a55e-5e4848a700b1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7c21-be1c-dc8429881ab8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_019d0bd2-921f-7fe1-9fe8-35cad7ae897c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7c21-be1c-dc8429881ab8" xlink:to="loc_us-gaap_AccountsPayableCurrent_019d0bd2-921f-7fe1-9fe8-35cad7ae897c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_019d0bd2-921f-75f8-8b1c-2a1bb4c2847d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7c21-be1c-dc8429881ab8" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_019d0bd2-921f-75f8-8b1c-2a1bb4c2847d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_AccruedResearchAndDevelopmentExpenses_019d0bd2-921f-7f72-8f7b-7c982d40c08d" xlink:href="dtil-20260331.xsd#dtil_AccruedResearchAndDevelopmentExpenses"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7c21-be1c-dc8429881ab8" xlink:to="loc_dtil_AccruedResearchAndDevelopmentExpenses_019d0bd2-921f-7f72-8f7b-7c982d40c08d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_019d0bd2-921f-7c27-b312-23b077dc707c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7c21-be1c-dc8429881ab8" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_019d0bd2-921f-7c27-b312-23b077dc707c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesCurrent_019d0bd2-921f-7911-a729-cc5e7993bd04" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherLiabilitiesCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_019d0bd2-921f-7c21-be1c-dc8429881ab8" xlink:to="loc_us-gaap_OtherLiabilitiesCurrent_019d0bd2-921f-7911-a729-cc5e7993bd04" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/CONDENSEDSTATEMENTSOFOPERATIONS" xlink:type="simple" xlink:href="dtil-20260331.xsd#CONDENSEDSTATEMENTSOFOPERATIONS"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/CONDENSEDSTATEMENTSOFOPERATIONS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_019dd851-e7ad-72bf-bd4f-e47d80df4788" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_019dd851-e7ad-7859-999c-77f2558c0e05" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_019dd851-e7ad-72bf-bd4f-e47d80df4788" xlink:to="loc_us-gaap_OtherNonoperatingIncomeExpense_019dd851-e7ad-7859-999c-77f2558c0e05" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019dd851-e7ad-713b-9657-1e5b3caf6209" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_019dd851-e7ad-72bf-bd4f-e47d80df4788" xlink:to="loc_us-gaap_OperatingIncomeLoss_019dd851-e7ad-713b-9657-1e5b3caf6209" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherNonoperatingIncomeExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromEquityMethodInvestments_019d0bd2-921f-7883-aa49-141d7a98c902" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:to="loc_us-gaap_IncomeLossFromEquityMethodInvestments_019d0bd2-921f-7883-aa49-141d7a98c902" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiGainLoss_019d0bd2-921f-7ad5-acd1-a90965fb3f57" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EquitySecuritiesFvNiGainLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:to="loc_us-gaap_EquitySecuritiesFvNiGainLoss_019d0bd2-921f-7ad5-acd1-a90965fb3f57" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_GainLossOnChangeInFairValueOfWarrantLiability_019d0bd2-921f-7417-883c-349102b4cd61" xlink:href="dtil-20260331.xsd#dtil_GainLossOnChangeInFairValueOfWarrantLiability"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:to="loc_dtil_GainLossOnChangeInFairValueOfWarrantLiability_019d0bd2-921f-7417-883c-349102b4cd61" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpenseNonoperating_019d0bd2-921f-77df-a63d-0dfe4ef25fc7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InterestExpenseNonoperating"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:to="loc_us-gaap_InterestExpenseNonoperating_019d0bd2-921f-77df-a63d-0dfe4ef25fc7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestIncomeOther_019d0bd2-921f-7c0c-b11e-87f049d11493" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_InterestIncomeOther"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:to="loc_us-gaap_InterestIncomeOther_019d0bd2-921f-7c0c-b11e-87f049d11493" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainLossOnDispositionOfAssets1_019d0bd2-921f-7002-8b4b-82bb99130f09" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GainLossOnDispositionOfAssets1"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OtherNonoperatingIncomeExpense_019d0bd2-921f-7085-ab15-d04fad642363" xlink:to="loc_us-gaap_GainLossOnDispositionOfAssets1_019d0bd2-921f-7002-8b4b-82bb99130f09" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_019d0bd2-921f-71f3-a6eb-d74202ca2776" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-921f-73c3-be26-c1df3c08da84" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_019d0bd2-921f-71f3-a6eb-d74202ca2776" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-921f-73c3-be26-c1df3c08da84" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_019d0bd2-921f-76b8-aac0-ed050e2e5d85" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_019d0bd2-921f-71f3-a6eb-d74202ca2776" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_019d0bd2-921f-76b8-aac0-ed050e2e5d85" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_019d0bd2-921f-7b07-bd5d-0633f184da65" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_019d0bd2-921f-7bbb-97ed-f3a295e0ae29" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_019d0bd2-921f-7b07-bd5d-0633f184da65" xlink:to="loc_us-gaap_OperatingExpenses_019d0bd2-921f-7bbb-97ed-f3a295e0ae29" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019d0bd2-921f-7b80-9d86-857da3bfd658" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_019d0bd2-921f-7b07-bd5d-0633f184da65" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_019d0bd2-921f-7b80-9d86-857da3bfd658" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/CONDENSEDSTATEMENTSOFCASHFLOWS" xlink:type="simple" xlink:href="dtil-20260331.xsd#CONDENSEDSTATEMENTSOFCASHFLOWS"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/CONDENSEDSTATEMENTSOFCASHFLOWS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_019d0bd2-921f-75b1-bc0d-900d716bbbde" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019d0bd2-921f-7451-b1c6-800b0007fae7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_019d0bd2-921f-75b1-bc0d-900d716bbbde" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_019d0bd2-921f-7451-b1c6-800b0007fae7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCash_019d0bd2-921f-76b4-a99a-cd21a7f36eb9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_RestrictedCash"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_019d0bd2-921f-75b1-bc0d-900d716bbbde" xlink:to="loc_us-gaap_RestrictedCash_019d0bd2-921f-76b4-a99a-cd21a7f36eb9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019d0bd2-921f-786b-81e9-cd68db7bdacc" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireProductiveAssets_019d0bd2-921f-78c6-8e41-e52ed60e6481" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_PaymentsToAcquireProductiveAssets"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019d0bd2-921f-786b-81e9-cd68db7bdacc" xlink:to="loc_us-gaap_PaymentsToAcquireProductiveAssets_019d0bd2-921f-78c6-8e41-e52ed60e6481" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_PaymentsToPurchaseIntangibleAssets_019d0bd2-921f-72c9-a9eb-f3c1ee4b024b" xlink:href="dtil-20260331.xsd#dtil_PaymentsToPurchaseIntangibleAssets"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019d0bd2-921f-786b-81e9-cd68db7bdacc" xlink:to="loc_dtil_PaymentsToPurchaseIntangibleAssets_019d0bd2-921f-72c9-a9eb-f3c1ee4b024b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromSaleOfMachineryAndEquipment_019d0bd2-921f-7555-9e1c-512c8f9acaec" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProceedsFromSaleOfMachineryAndEquipment"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019d0bd2-921f-786b-81e9-cd68db7bdacc" xlink:to="loc_us-gaap_ProceedsFromSaleOfMachineryAndEquipment_019d0bd2-921f-7555-9e1c-512c8f9acaec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019d0bd2-921f-7d5e-bfe1-84aed4aa913d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_ProceedsFromEmployeeStockPurchasePlan_019d0bd2-921f-7bf9-9fe3-d363021357be" xlink:href="dtil-20260331.xsd#dtil_ProceedsFromEmployeeStockPurchasePlan"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019d0bd2-921f-7d5e-bfe1-84aed4aa913d" xlink:to="loc_dtil_ProceedsFromEmployeeStockPurchasePlan_019d0bd2-921f-7bf9-9fe3-d363021357be" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_ProceedsFromIssuanceOfCommonStockThroughATMFacilityNetOfIssuanceCost_019d0bd2-921f-77c6-8439-cc9e4bc934e7" xlink:href="dtil-20260331.xsd#dtil_ProceedsFromIssuanceOfCommonStockThroughATMFacilityNetOfIssuanceCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019d0bd2-921f-7d5e-bfe1-84aed4aa913d" xlink:to="loc_dtil_ProceedsFromIssuanceOfCommonStockThroughATMFacilityNetOfIssuanceCost_019d0bd2-921f-77c6-8439-cc9e4bc934e7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromMinorityShareholders_019d0bd2-921f-7985-9ae1-832dfa020d7c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProceedsFromMinorityShareholders"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019d0bd2-921f-7d5e-bfe1-84aed4aa913d" xlink:to="loc_us-gaap_ProceedsFromMinorityShareholders_019d0bd2-921f-7985-9ae1-832dfa020d7c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019d0bd2-921f-7533-b781-d6f6e0f188af" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-7e14-8aac-36c1b38863c2" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019d0bd2-921f-7533-b781-d6f6e0f188af" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-7e14-8aac-36c1b38863c2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019d0bd2-921f-7260-9974-f1876355f909" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019d0bd2-921f-7533-b781-d6f6e0f188af" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_019d0bd2-921f-7260-9974-f1876355f909" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019d0bd2-921f-742b-9b16-91ed0c542805" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_019d0bd2-921f-7533-b781-d6f6e0f188af" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_019d0bd2-921f-742b-9b16-91ed0c542805" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProfitLoss_019d0bd2-921f-7df4-9077-008b8747ab8d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ProfitLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_ProfitLoss_019d0bd2-921f-7df4-9077-008b8747ab8d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_019d0bd2-921f-7128-af58-982574b084bb" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_DepreciationAndAmortization_019d0bd2-921f-7128-af58-982574b084bb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_019d0bd2-921f-7819-a6f3-d3d157beb842" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_ShareBasedCompensation_019d0bd2-921f-7819-a6f3-d3d157beb842" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnSalesOfAssets_019d0bd2-921f-7cf0-9108-78bf0b3f7289" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GainsLossesOnSalesOfAssets"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_GainsLossesOnSalesOfAssets_019d0bd2-921f-7cf0-9108-78bf0b3f7289" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfDebtDiscountPremium_019d0bd2-921f-716e-895c-d78bf857ef97" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AmortizationOfDebtDiscountPremium"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_AmortizationOfDebtDiscountPremium_019d0bd2-921f-716e-895c-d78bf857ef97" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_019d0bd2-921f-76c5-8354-327a78cf18d8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAssetAmortizationExpense_019d0bd2-921f-76c5-8354-327a78cf18d8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueOptionChangesInFairValueGainLoss1_019d0bd2-921f-7675-a05e-df0f0cbd6a35" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FairValueOptionChangesInFairValueGainLoss1"/>
    <link:calculationArc order="7" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_FairValueOptionChangesInFairValueGainLoss1_019d0bd2-921f-7675-a05e-df0f0cbd6a35" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromEquityMethodInvestments_019d0bd2-921f-7bd1-b7f4-db8302d436c7" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncomeLossFromEquityMethodInvestments"/>
    <link:calculationArc order="8" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncomeLossFromEquityMethodInvestments_019d0bd2-921f-7bd1-b7f4-db8302d436c7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_AmortizationOfDiscountOnNoteReceivable_019d0bd2-921f-7668-9192-d8a2b784c9a8" xlink:href="dtil-20260331.xsd#dtil_AmortizationOfDiscountOnNoteReceivable"/>
    <link:calculationArc order="9" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_dtil_AmortizationOfDiscountOnNoteReceivable_019d0bd2-921f-7668-9192-d8a2b784c9a8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_GainLossOnChangeInFairValueOfWarrantLiability_019d0bd2-921f-7613-8ac9-2d77f8558c8d" xlink:href="dtil-20260331.xsd#dtil_GainLossOnChangeInFairValueOfWarrantLiability"/>
    <link:calculationArc order="10" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_dtil_GainLossOnChangeInFairValueOfWarrantLiability_019d0bd2-921f-7613-8ac9-2d77f8558c8d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidExpense_019d0bd2-921f-77c5-8bdf-23ce2ecd9315" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInPrepaidExpense"/>
    <link:calculationArc order="11" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidExpense_019d0bd2-921f-77c5-8bdf-23ce2ecd9315" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInMarketableSecuritiesRestricted_019d0bd2-921f-776f-bd35-ce4d1a06ff32" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInMarketableSecuritiesRestricted"/>
    <link:calculationArc order="12" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInMarketableSecuritiesRestricted_019d0bd2-921f-776f-bd35-ce4d1a06ff32" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_019d0bd2-921f-7552-85f9-afa42155046a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInAccountsReceivable"/>
    <link:calculationArc order="13" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsReceivable_019d0bd2-921f-7552-85f9-afa42155046a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInContractWithCustomerAsset_019d0bd2-921f-7def-9e2b-524a9e31bda6" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInContractWithCustomerAsset"/>
    <link:calculationArc order="14" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInContractWithCustomerAsset_019d0bd2-921f-7def-9e2b-524a9e31bda6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_IncreaseDecreaseInOtherAssetsAndOtherCurrentAssets_019d0bd2-921f-7c8f-8688-ac67e8f97d9e" xlink:href="dtil-20260331.xsd#dtil_IncreaseDecreaseInOtherAssetsAndOtherCurrentAssets"/>
    <link:calculationArc order="15" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_dtil_IncreaseDecreaseInOtherAssetsAndOtherCurrentAssets_019d0bd2-921f-7c8f-8688-ac67e8f97d9e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_019d0bd2-921f-77af-a3a3-d9acb743fc63" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="16" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_019d0bd2-921f-77af-a3a3-d9acb743fc63" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_IncreaseDecreaseInAccruedOtherExpensesAndOtherCurrentLiabilities_019d0bd2-921f-7585-91db-ca53bc1da39a" xlink:href="dtil-20260331.xsd#dtil_IncreaseDecreaseInAccruedOtherExpensesAndOtherCurrentLiabilities"/>
    <link:calculationArc order="17" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_dtil_IncreaseDecreaseInAccruedOtherExpensesAndOtherCurrentLiabilities_019d0bd2-921f-7585-91db-ca53bc1da39a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInContractWithCustomerLiability_019d0bd2-921f-78e0-84fc-d79a5192eeed" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInContractWithCustomerLiability"/>
    <link:calculationArc order="18" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInContractWithCustomerLiability_019d0bd2-921f-78e0-84fc-d79a5192eeed" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_019d0bd2-921f-70f6-885d-46daa6f0ffa8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_IncreaseDecreaseInOperatingLeaseLiability"/>
    <link:calculationArc order="19" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_019d0bd2-921f-79b7-9d9b-e106c447b276" xlink:to="loc_us-gaap_IncreaseDecreaseInOperatingLeaseLiability_019d0bd2-921f-70f6-885d-46daa6f0ffa8" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/FAIRVALUEMEASUREMENTSScheduleofAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails" xlink:type="simple" xlink:href="dtil-20260331.xsd#FAIRVALUEMEASUREMENTSScheduleofAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/FAIRVALUEMEASUREMENTSScheduleofAssetsandLiabilitiesMeasuredatFairValueonRecurringBasisDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesFairValueDisclosure_019d0bd2-921f-7b2f-9d96-63ef2ad405c1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LiabilitiesFairValueDisclosure"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_019d0bd2-921f-7f39-a882-1dd56627428c" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_FinancialLiabilitiesFairValueDisclosure"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosure_019d0bd2-921f-7b2f-9d96-63ef2ad405c1" xlink:to="loc_us-gaap_FinancialLiabilitiesFairValueDisclosure_019d0bd2-921f-7f39-a882-1dd56627428c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeLiabilities_019d0bd2-921f-7b7c-b7c0-ea72364fd464" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DerivativeLiabilities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesFairValueDisclosure_019d0bd2-921f-7b2f-9d96-63ef2ad405c1" xlink:to="loc_us-gaap_DerivativeLiabilities_019d0bd2-921f-7b7c-b7c0-ea72364fd464" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsFairValueDisclosure_019d0bd2-921f-79f3-8c42-9be8d25a5f89" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_AssetsFairValueDisclosure"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019d0bd2-921f-73c3-94fa-954350b9c273" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsFairValueDisclosure_019d0bd2-921f-79f3-8c42-9be8d25a5f89" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_019d0bd2-921f-73c3-94fa-954350b9c273" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_019d0bd2-921f-7914-968a-900c99bac514" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsFairValueDisclosure_019d0bd2-921f-79f3-8c42-9be8d25a5f89" xlink:to="loc_us-gaap_EquitySecuritiesFvNiCurrentAndNoncurrent_019d0bd2-921f-7914-968a-900c99bac514" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/COMMITMENTSANDCONTINGENCIESComponentsofLeaseExpenseDetails" xlink:type="simple" xlink:href="dtil-20260331.xsd#COMMITMENTSANDCONTINGENCIESComponentsofLeaseExpenseDetails"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/COMMITMENTSANDCONTINGENCIESComponentsofLeaseExpenseDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseCost_019d0bd2-921f-7f8b-8aac-096cbaa7f783" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LeaseCost"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseCost_019d0bd2-921f-730e-8330-2ce585d58d9b" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseCost"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_019d0bd2-921f-7f8b-8aac-096cbaa7f783" xlink:to="loc_us-gaap_OperatingLeaseCost_019d0bd2-921f-730e-8330-2ce585d58d9b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShortTermLeaseCost_019d0bd2-921f-7517-ae90-2497f1464fb9" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ShortTermLeaseCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_019d0bd2-921f-7f8b-8aac-096cbaa7f783" xlink:to="loc_us-gaap_ShortTermLeaseCost_019d0bd2-921f-7517-ae90-2497f1464fb9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableLeaseCost_019d0bd2-921f-713f-939e-5ae43acd9d45" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_VariableLeaseCost"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_019d0bd2-921f-7f8b-8aac-096cbaa7f783" xlink:to="loc_us-gaap_VariableLeaseCost_019d0bd2-921f-713f-939e-5ae43acd9d45" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SubleaseIncome_019d0bd2-921f-705a-9c29-575e64fc5437" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_SubleaseIncome"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LeaseCost_019d0bd2-921f-7f8b-8aac-096cbaa7f783" xlink:to="loc_us-gaap_SubleaseIncome_019d0bd2-921f-705a-9c29-575e64fc5437" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/COMMITMENTSANDCONTINGENCIESScheduleofFutureLeasePaymentsunderNonCancelableLeasesDetails" xlink:type="simple" xlink:href="dtil-20260331.xsd#COMMITMENTSANDCONTINGENCIESScheduleofFutureLeasePaymentsunderNonCancelableLeasesDetails"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/COMMITMENTSANDCONTINGENCIESScheduleofFutureLeasePaymentsunderNonCancelableLeasesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d6b63-f4de-75c4-bc31-908417a59dd1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_019d6b63-f4de-7e4e-b182-2defd97b6420" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d6b63-f4de-75c4-bc31-908417a59dd1" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_019d6b63-f4de-7e4e-b182-2defd97b6420" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_019d6b63-f4de-7fbd-9fcd-1a9773189816" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d6b63-f4de-75c4-bc31-908417a59dd1" xlink:to="loc_us-gaap_OperatingLeaseLiability_019d6b63-f4de-7fbd-9fcd-1a9773189816" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/COMMITMENTSANDCONTINGENCIESScheduleofFutureLeasePaymentsunderNonCancelableLeasesDetails_1" xlink:type="simple" xlink:href="dtil-20260331.xsd#COMMITMENTSANDCONTINGENCIESScheduleofFutureLeasePaymentsunderNonCancelableLeasesDetails_1"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/COMMITMENTSANDCONTINGENCIESScheduleofFutureLeasePaymentsunderNonCancelableLeasesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d0bd2-921f-718f-8ab4-8d5d48e65fc0" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_019d0bd2-921f-7927-86d1-cffc0beda34a" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d0bd2-921f-718f-8ab4-8d5d48e65fc0" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_019d0bd2-921f-7927-86d1-cffc0beda34a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_019d0bd2-921f-79ff-bea7-d236135ef4b1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d0bd2-921f-718f-8ab4-8d5d48e65fc0" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_019d0bd2-921f-79ff-bea7-d236135ef4b1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_019d0bd2-921f-7da7-baa7-d5805f210483" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d0bd2-921f-718f-8ab4-8d5d48e65fc0" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_019d0bd2-921f-7da7-baa7-d5805f210483" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_019d0bd2-921f-7170-ad21-dce3867888e1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_019d0bd2-921f-718f-8ab4-8d5d48e65fc0" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_019d0bd2-921f-7170-ad21-dce3867888e1" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/EARNINGSPERSHAREDetails" xlink:type="simple" xlink:href="dtil-20260331.xsd#EARNINGSPERSHAREDetails"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/EARNINGSPERSHAREDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019d0bd2-921f-7295-aaa8-fa27c62394f1" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019d0bd2-921f-7f2c-96e1-2a39f670122d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019d0bd2-921f-7295-aaa8-fa27c62394f1" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_019d0bd2-921f-7f2c-96e1-2a39f670122d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_019d0bd2-921f-77dc-b7f7-316fdeed2863" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_WeightedAverageNumberOfDilutedSharesOutstanding_019d0bd2-921f-7295-aaa8-fa27c62394f1" xlink:to="loc_us-gaap_WeightedAverageNumberDilutedSharesOutstandingAdjustment_019d0bd2-921f-77dc-b7f7-316fdeed2863" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://precisionbiosciences.com/role/SEGMENTREPORTINGSummaryofSignificantResearchandDevelopmentandGeneralandAdministrativeExpensetoCODMDetails" xlink:type="simple" xlink:href="dtil-20260331.xsd#SEGMENTREPORTINGSummaryofSignificantResearchandDevelopmentandGeneralandAdministrativeExpensetoCODMDetails"/>
  <link:calculationLink xlink:role="http://precisionbiosciences.com/role/SEGMENTREPORTINGSummaryofSignificantResearchandDevelopmentandGeneralandAdministrativeExpensetoCODMDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_019d0bd2-92c0-79e1-bb81-00752badfa1d" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_EmployeeRelatedCostsGeneralAndAdministrativeExpense_019d0bd2-92c0-7a29-8c6d-9eca1fdce52a" xlink:href="dtil-20260331.xsd#dtil_EmployeeRelatedCostsGeneralAndAdministrativeExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_GeneralAndAdministrativeExpense_019d0bd2-92c0-79e1-bb81-00752badfa1d" xlink:to="loc_dtil_EmployeeRelatedCostsGeneralAndAdministrativeExpense_019d0bd2-92c0-7a29-8c6d-9eca1fdce52a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_ConsultingAndProfessionalServices_019d0bd2-92c0-7e8f-a44b-a26bcaa32daa" xlink:href="dtil-20260331.xsd#dtil_ConsultingAndProfessionalServices"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_GeneralAndAdministrativeExpense_019d0bd2-92c0-79e1-bb81-00752badfa1d" xlink:to="loc_dtil_ConsultingAndProfessionalServices_019d0bd2-92c0-7e8f-a44b-a26bcaa32daa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherCostAndExpenseOperating_019d0bd2-92c0-72f6-9e7b-4f04755e8dcb" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_OtherCostAndExpenseOperating"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_GeneralAndAdministrativeExpense_019d0bd2-92c0-79e1-bb81-00752badfa1d" xlink:to="loc_us-gaap_OtherCostAndExpenseOperating_019d0bd2-92c0-72f6-9e7b-4f04755e8dcb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_ExternalDevelopmentCostsCredits_019d0bd2-92c0-70e3-95ae-0625e6627cea" xlink:href="dtil-20260331.xsd#dtil_ExternalDevelopmentCostsCredits"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_dtil_ExternalDevelopmentCostsCredits_019d0bd2-92c0-70e3-95ae-0625e6627cea" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_EmployeeRelatedCostsResearchAndDevelopmentIncludingShare-BasedCompensation_019d0bd2-92c0-7733-b434-44d35c35001b" xlink:href="dtil-20260331.xsd#dtil_EmployeeRelatedCostsResearchAndDevelopmentIncludingShare-BasedCompensation"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_dtil_EmployeeRelatedCostsResearchAndDevelopmentIncludingShare-BasedCompensation_019d0bd2-92c0-7733-b434-44d35c35001b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_LaboratorySuppliesAndServices_019d0bd2-92c0-72b9-b9db-d0d9c08ba5fb" xlink:href="dtil-20260331.xsd#dtil_LaboratorySuppliesAndServices"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_dtil_LaboratorySuppliesAndServices_019d0bd2-92c0-72b9-b9db-d0d9c08ba5fb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_CMOsAndOutsourcedResearchAndDevelopment_019d0bd2-92c0-7999-990f-af09696ec684" xlink:href="dtil-20260331.xsd#dtil_CMOsAndOutsourcedResearchAndDevelopment"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_dtil_CMOsAndOutsourcedResearchAndDevelopment_019d0bd2-92c0-7999-990f-af09696ec684" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_FacilityRelatedCostsLaboratoryEquipmentAndMaintenance_019d0bd2-92c0-7399-948f-03593a594f14" xlink:href="dtil-20260331.xsd#dtil_FacilityRelatedCostsLaboratoryEquipmentAndMaintenance"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_dtil_FacilityRelatedCostsLaboratoryEquipmentAndMaintenance_019d0bd2-92c0-7399-948f-03593a594f14" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_019d0bd2-92c0-7aad-a064-cdbbc8a5e0b8" xlink:href="https://xbrl.fasb.org/us-gaap/2025/elts/us-gaap-2025.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_us-gaap_DepreciationAndAmortization_019d0bd2-92c0-7aad-a064-cdbbc8a5e0b8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_dtil_LicensingFeesAndOtherResearchAndDevelopmentCosts_019d0bd2-92c0-763b-b0a3-b1f32c500bab" xlink:href="dtil-20260331.xsd#dtil_LicensingFeesAndOtherResearchAndDevelopmentCosts"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="https://xbrl.org/2023/arcrole/summation-item" xlink:from="loc_us-gaap_ResearchAndDevelopmentExpense_019d0bd2-92c0-7877-9ce2-abe112f6ff96" xlink:to="loc_dtil_LicensingFeesAndOtherResearchAndDevelopmentCosts_019d0bd2-92c0-763b-b0a3-b1f32c500bab" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
