Exhibit 8.1
Pyxis Tankers Inc.
List of Subsidiaries
| Company Name | Ownership * | Jurisdiction of Incorporation | ||
| Secondone Corporation Ltd. ** | 100% | Republic of the Marshall Islands | ||
| Thirdone Corporation Ltd. ** | 100% | Republic of the Marshall Islands | ||
| Fourthone Corporation Ltd. ** | 100% | Republic of the Marshall Islands | ||
| Sixthone Corp. ** | 100% | Republic of the Marshall Islands | ||
| Seventhone Corp. | 100% | Republic of the Marshall Islands | ||
| Eighthone Corp. ** | 100% | Republic of the Marshall Islands | ||
| Tenthone | 100% | Republic of the Marshall Islands | ||
| Eleventhone | 100% | Republic of the Marshall Islands | ||
| Twelfthone | 100% | Republic of the Marshall Islands | ||
| Maritime Technologies Corp. | 100% | Delaware, U.S.A. | ||
| Drykon Maritime Inc. | 60% | Republic of the Marshall Islands | ||
| Dryone Corp. | 60% | Republic of the Marshall Islands | ||
| Drytwo Corp. | 100% | Republic of the Marshall Islands | ||
| Accuship Maritime Ltd. | 60% | Republic of the Marshall Islands | ||
| Drythree Corp. | 60% | Republic of the Marshall Islands | ||
| Dryfour Corp. | 100% | Republic of the Marshall Islands |
*Ownership of each subsidiary by Pyxis Tankers Inc.
** “Pyxis Delta”, “Northsea Alpha”, “Northsea Beta”, “Pyxis Malou” and “Pyxis Epsilon” were sold to unaffiliated third parties on January 13, 2020, January 28, 2022, March 1, 2022, March 23, 2023 and December 15, 2023 respectively.