Exhibit 8.1

Subsidiaries of Galapagos NV on 31/12/2025

Name of Subsidiary

  ​ ​ ​

Jurisdiction of Incorporation or Organization

Galapagos B.V.

The Netherlands

Galapagos U.K. Ltd

United Kingdom

Galapagos SASU

France

Galapagos GmbH

Switzerland

Galapagos Real Estate Netherlands B.V.

The Netherlands

GLPG US Holding Inc. (formerly Galapagos, Inc.)

United States

GLPG US Inc. (formerly AboundBio, Inc.)

United States

Xenometrix, Inc. (in liquidation)

United States

Galapagos Holding Pte. Ltd.

Singapore

Galapagos (Shanghai) Bioscience Co. Ltd.

China

Galapagos Cell Therapeutics NV

Belgium

Lakefront Biotherapeutics, Inc.

United States

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