11. Subsequent Events

In preparing these financial statements, Management has evaluated the financial statements for the year ended December 31, 2025 for subsequent events through the date of this filing and noted no material events requiring either recognition through the date of the filing or disclosure herein for the Fund other than as noted below.

Prior to January 20, 2026, the Trust’s Index Constituents were those included in the NCIUSS. Effective as of the Transition Date, the index changed to the NCIS. The NCIUSS represents the daily closing value of the NCIUS, and the NCIS represents the daily closing value of the NCI. The NCIUSS and the NCIS apply substantially identical methodologies, reflect the same constituents, and are both designed to measure the performance of a material portion of the overall crypto asset market.

On January 20, 2026, the Sponsor caused a Certificate of Amendment to the Trust’s Certificate of Trust to be filed with the Secretary of State of the State of Delaware to change the name of the Trust from “Hashdex Nasdaq Crypto Index US ETF” to “Hashdex Nasdaq CME Crypto Index ETF.” On the same date, the Sponsor and the Trustee entered into a Fifth Amended and Restated Trust Agreement, which made conforming changes to the Fourth Amended and Restated Trust Agreement to reflect the name change and the transition of the Trust’s underlying index from the Nasdaq Crypto US Settlement Price Index (NCIUSS) to the Nasdaq CME Crypto Settlement Price Index (NCIS), effective as of such date. Both indexes apply substantially identical methodologies and reflected the same constituents and weightings as of the transition date. The index transition did not result in any material change to the Trust’s investment objective, portfolio composition, or risk profile. The foregoing events were reported on Form 8-K filed with the SEC on January 20, 2026.

On February 24, 2026, the Trust and the Sponsor entered into Amendment #1 to the Authorized Participant Agreement with Virtu Americas LLC to permit the creation and redemption of Baskets through in-kind transfers of digital assets. The Amendment was reported on Form 8-K filed with the SEC on February 27, 2026.

On March 5, 2026, Hashdex Ltd., the controlling entity of the Sponsor, announced the following leadership changes at the Hashdex group level, effective immediately: Marcelo Sampaio, who served as Chief Executive Officer and President, transitioned to the role of Executive Chairman; Bruno Caratori, Co-Founder and Chief Operating Officer, was appointed Global Chief Executive Officer; and Mick McLaughlin was appointed U.S. Chief Executive Officer. These changes were made at the Hashdex Ltd. level and do not affect the Sponsor. Bruno Sousa continues to serve as the Trust’s Principal Executive Officer, and Samir Kerbage continues to serve as the Trust’s Principal Financial Officer and Principal Accounting Officer. This event was reported on Form 8-K filed with the SEC on March 11, 2026.

On March 13, 2026, the Sponsor and the Trust entered into the Second Amendment to the Sponsor Agreement, originally dated January 27, 2025 and previously amended on November 12, 2025. The Second Amendment reduces the Management Fee from 0.50% to 0.25% per annum of the Trust’s net asset value, effective as of March 16, 2026. Prior to the Second Amendment, the Sponsor had been waiving a portion of the Management Fee pursuant to the prior amendment, resulting in an effective fee of 0.25% per annum through December 31, 2026. As a result of the fee reduction, the fee waiver provision is no longer in effect. This event was reported on Form 8-K filed with the SEC on March 16, 2026.