S-3 S-3 EX-FILING FEES 0001426800 ASSEMBLY BIOSCIENCES, INC. N/A 0.0001381 0.0001381 0.0001381 0.0001381 0.0001381 0.0001381 Y N 0001426800 2026-03-19 2026-03-19 0001426800 1 2026-03-19 2026-03-19 0001426800 2 2026-03-19 2026-03-19 0001426800 3 2026-03-19 2026-03-19 0001426800 4 2026-03-19 2026-03-19 0001426800 5 2026-03-19 2026-03-19 0001426800 6 2026-03-19 2026-03-19 0001426800 7 2026-03-19 2026-03-19 0001426800 1 2026-03-19 2026-03-19 0001426800 2 2026-03-19 2026-03-19 iso4217:USD xbrli:pure xbrli:shares

Calculation of Filing Fee Tables

S-3

ASSEMBLY BIOSCIENCES, INC.

Table 1: Newly Registered and Carry Forward Securities ☐Not Applicable

Security Type

Security Class Title

Fee Calculation or Carry Forward Rule

Amount Registered

Proposed Maximum Offering Price Per Unit

Maximum Aggregate Offering Price

Fee Rate

Amount of Registration Fee

Carry Forward Form Type

Carry Forward File Number

Carry Forward Initial Effective Date

Filing Fee Previously Paid in Connection with Unsold Securities to be Carried Forward

Newly Registered Securities
Equity Common Stock, par value $0.001 per share 457(o)
Equity Preferred Stock, par value $0.001 per share 457(o)
Debt Debt Securities 457(o)
Other Warrants 457(o)
Other Subscription Rights 457(o)
Other Units 457(o)
Fees to be Paid 1 Unallocated (Universal) Shelf 457(o) $ 400,000,000.00 0.0001381 $ 55,240.00
Fees Previously Paid
Carry Forward Securities
Carry Forward Securities

Total Offering Amounts:

$ 400,000,000.00

$ 55,240.00

Total Fees Previously Paid:

$ 0.00

Total Fee Offsets:

$ 13,877.69

Net Fee Due:

$ 41,362.31

Offering Note

1

1) Assembly Biosciences, Inc. (the "Company") is hereby registering an indeterminate amount and number of each applicable identified class of the identified securities up to a proposed maximum aggregate offering price of $400,000,000, which may be offered from time to time at indeterminate prices, including securities that may be purchased by underwriters. The Company has estimated the proposed maximum aggregate offering price solely for the purpose of calculating the registration fee pursuant to Rule 457(o) under the Securities Act of 1933, as amended (the "Securities Act"). Securities registered hereunder may be sold separately, together or as units with other securities registered hereunder. (2) Pursuant to Rule 416 under the Securities, this registration statement shall also cover any additional shares of the registrant's securities that become issuable by reason of any share splits, share dividends or similar transactions. (3) Estimated solely for the purpose of calculating the registration fee. No separate consideration will be received for shares of common stock that are issued upon conversion of debt securities or preferred stock or upon exercise of common stock warrants registered hereunder. The aggregate maximum offering price of all securities issued pursuant to this registration statement will not exceed $400,000,000. (4) Any securities registered under the registration statement may be sold separately or as units with other securities registered under the registration statement.
Table 2: Fee Offset Claims and Sources ☐Not Applicable
Registrant or Filer Name Form or Filing Type File Number Initial Filing Date Filing Date Fee Offset Claimed Security Type Associated with Fee Offset Claimed Security Title Associated with Fee Offset Claimed Unsold Securities Associated with Fee Offset Claimed Unsold Aggregate Offering Amount Associated with Fee Offset Claimed Fee Paid with Fee Offset Source
Rules 457(b) and 0-11(a)(2)
Fee Offset Claims
Fee Offset Sources
Rule 457(p)
Fee Offset Claims 1 Assembly Biosciences, Inc. S-3 333-270760 03/22/2023 $ 13,877.69 Unallocated (Universal) Shelf Unallocated (Universal) Shelf $ 125,931,832.60
Fee Offset Sources 2 Assembly Biosciences, Inc. S-3 333-270760 03/22/2023 $ 16,530.00

Rule 457(p) Statement of Withdrawal, Termination, or Completion:

1

Pursuant to Rule 457(p) under the Securities Act, a registration fee of $16,530 was paid with respect to securities available for issuance under a Registration Statement on Form S-3 (File No. 333-270760) filed by the registrant on March 22, 2023 and declared effective on April 14, 2023 (the Prior Registration Statement). The Registrant hereby offsets the total registration fee of $55,240 due under this registration statement by $13,877.69 (calculated at the fee rate in effect at the date of the Prior Registration Statement of $110.20 per million dollars), which represents the portion of the registration fee previously paid with respect to $125,931,832.63 of unsold securities previously registered under the Prior Registration Statement. The offering of the unsold securities registered under the Prior Registration Statement has been terminated.

Offset Note

2

Pursuant to Rule 457(p) under the Securities Act, a registration fee of $16,530 was paid with respect to securities available for issuance under a Registration Statement on Form S-3 (File No. 333-270760) filed by the registrant on March 22, 2023 and declared effective on April 14, 2023 (the Prior Registration Statement). The Registrant hereby offsets the total registration fee of $55,240 due under this registration statement by $13,877.69 (calculated at the fee rate in effect at the date of the Prior Registration Statement of $110.20 per million dollars), which represents the portion of the registration fee previously paid with respect to $125,931,832.63 of unsold securities previously registered under the Prior Registration Statement. The offering of the unsold securities registered under the Prior Registration Statement has been terminated.
Table 3: Combined Prospectuses ☑Not Applicable

Security Type

Security Class Title

Amount of Securities Previously Registered

Maximum Aggregate Offering Price of Securities Previously Registered

Form Type

File Number

Initial Effective Date