Offerings	Mar. 13, 2026 USD ($) shares
Offering: 1
Offering:
Fee Previously Paid	false
Other Rule	true
Security Type	Equity
Security Class Title	Common stock, $0.0001 par value per share, 2024 Equity Incentive Plan
Amount Registered \| shares	1,596,176
Proposed Maximum Offering Price per Unit	21.14
Maximum Aggregate Offering Price	$ 33,743,160.64
Fee Rate	0.01381%
Amount of Registration Fee	$ 4,659.93
Offering Note	Pursuant to Rule 416(a) promulgated under the Securities Act of 1933, as amended (the "Securities Act"), this Registration Statement shall also cover any additional shares of common stock of Alto Neuroscience, Inc. (the "Registrant") that become issuable under the Registrant's 2024 Equity Incentive Plan (the "2024 Plan") by reason of any stock dividend, stock split, recapitalization, or other similar transaction effected that results in an increase to the number of outstanding shares of the Registrant's common stock, as applicable. The amount registered represents shares of common stock that were added to the shares reserved for future issuance under the 2024 Plan on January 1, 2026, pursuant to an evergreen provision contained in the 2024 Plan. Pursuant to such provision, the number of shares reserved for issuance under the 2024 Plan will automatically increase on January 1 of each year for a period of 10 years, beginning on January 1, 2025 and continuing through (and including) January 1, 2034, in an amount equal to 5% of the total number of shares of the Registrant's common stock outstanding on December 31 of the immediately preceding year, except that, before the date of any such increase, the Registrant's board of directors may determine that the increase for such year will be a lesser number of shares. The proposed maximum aggregate offering price per unit and proposed maximum aggregate offering price for the 1,596,176 shares of common stock reserved for grant under the 2024 Plan are estimated in accordance with Rules 457(c) and 457(h) solely for the purpose of calculating the registration fee on the basis of $21.14 per share, which is the average of the high and low selling prices per share of the Registrant's common stock on March 9, 2026 as reported on the New York Stock Exchange.
Offering: 2
Offering:
Fee Previously Paid	false
Other Rule	true
Security Type	Equity
Security Class Title	Common stock, $0.0001 par value per share, 2024 Employee Stock Purchase Plan
Amount Registered \| shares	319,235
Proposed Maximum Offering Price per Unit	17.97
Maximum Aggregate Offering Price	$ 5,736,652.95
Fee Rate	0.01381%
Amount of Registration Fee	$ 792.23
Offering Note	Pursuant to Rule 416(a) promulgated under the Securities Act, this Registration Statement shall also cover any additional shares of common stock of the Registrant that become issuable under the Registrant's 2024 Employee Stock Purchase Plan (the "2024 ESPP") by reason of any stock dividend, stock split, recapitalization, or other similar transaction effected that results in an increase to the number of outstanding shares of the Registrant's common stock, as applicable. The amount registered represents shares of common stock that were added to the shares reserved for future issuance under the 2024 ESPP on January 1, 2026, pursuant to an evergreen provision contained in the 2024 ESPP. Pursuant to such provision, the number of shares reserved for issuance under the 2024 ESPP will automatically increase on January 1 of each year for a period of 10 years, beginning on January 1, 2025 and continuing through (and including) January 1, 2034, by the lesser of (x) 1% of the total number of shares of the Registrant's common stock outstanding on December 31 of the immediately preceding year and (y) 750,000 shares, except that, before the date of any such increase, the Registrant's board of directors may determine that the increase for such year will be less than the amount set forth in clauses (x) and (y). The proposed maximum aggregate offering price per unit and proposed maximum aggregate offering price for the 319,235 shares of common stock reserved for grant under the 2024 ESPP are estimated in accordance with Rules 457(c) and 457(h) solely for the purpose of calculating the registration fee. The offering price per share and the aggregate offering price are based upon $17.97 per share, which is the average of the high and low selling prices per share of the Registrant's common stock on March 9, 2026 as reported on the New York Stock Exchange multiplied by 85%, which is the percentage of the price per share applicable to purchases under the 2024 ESPP.

Offerings

Mar. 13, 2026

USD ($)

Offering:

false

true

Equity

Common stock, $0.0001 par value per share, 2024 Equity Incentive Plan

Amount Registered | shares

1,596,176

Proposed Maximum Offering Price per Unit

21.14

Maximum Aggregate Offering Price

$ 33,743,160.64

Fee Rate

0.01381%

Amount of Registration Fee

$ 4,659.93

Offering Note

Pursuant to Rule 416(a) promulgated under the Securities Act of 1933, as amended (the "Securities Act"), this Registration Statement shall also cover any additional shares of common stock of Alto Neuroscience, Inc. (the "Registrant") that become issuable under the Registrant's 2024 Equity Incentive Plan (the "2024 Plan") by reason of any stock dividend, stock split, recapitalization, or other similar transaction effected that results in an increase to the number of outstanding shares of the Registrant's common stock, as applicable. The amount registered represents shares of common stock that were added to the shares reserved for future issuance under the 2024 Plan on January 1, 2026, pursuant to an evergreen provision contained in the 2024 Plan. Pursuant to such provision, the number of shares reserved for issuance under the 2024 Plan will automatically increase on January 1 of each year for a period of 10 years, beginning on January 1, 2025 and continuing through (and including) January 1, 2034, in an amount equal to 5% of the total number of shares of the Registrant's common stock outstanding on December 31 of the immediately preceding year, except that, before the date of any such increase, the Registrant's board of directors may determine that the increase for such year will be a lesser number of shares. The proposed maximum aggregate offering price per unit and proposed maximum aggregate offering price for the 1,596,176 shares of common stock reserved for grant under the 2024 Plan are estimated in accordance with Rules 457(c) and 457(h) solely for the purpose of calculating the registration fee on the basis of $21.14 per share, which is the average of the high and low selling prices per share of the Registrant's common stock on March 9, 2026 as reported on the New York Stock Exchange.

Offering:

false

true

Equity

Common stock, $0.0001 par value per share, 2024 Employee Stock Purchase Plan

Amount Registered | shares

319,235

Proposed Maximum Offering Price per Unit

17.97

Maximum Aggregate Offering Price

$ 5,736,652.95

Fee Rate

0.01381%

Amount of Registration Fee

$ 792.23

Offering Note

Pursuant to Rule 416(a) promulgated under the Securities Act, this Registration Statement shall also cover any additional shares of common stock of the Registrant that become issuable under the Registrant's 2024 Employee Stock Purchase Plan (the "2024 ESPP") by reason of any stock dividend, stock split, recapitalization, or other similar transaction effected that results in an increase to the number of outstanding shares of the Registrant's common stock, as applicable. The amount registered represents shares of common stock that were added to the shares reserved for future issuance under the 2024 ESPP on January 1, 2026, pursuant to an evergreen provision contained in the 2024 ESPP. Pursuant to such provision, the number of shares reserved for issuance under the 2024 ESPP will automatically increase on January 1 of each year for a period of 10 years, beginning on January 1, 2025 and continuing through (and including) January 1, 2034, by the lesser of (x) 1% of the total number of shares of the Registrant's common stock outstanding on December 31 of the immediately preceding year and (y) 750,000 shares, except that, before the date of any such increase, the Registrant's board of directors may determine that the increase for such year will be less than the amount set forth in clauses (x) and (y). The proposed maximum aggregate offering price per unit and proposed maximum aggregate offering price for the 319,235 shares of common stock reserved for grant under the 2024 ESPP are estimated in accordance with Rules 457(c) and 457(h) solely for the purpose of calculating the registration fee. The offering price per share and the aggregate offering price are based upon $17.97 per share, which is the average of the high and low selling prices per share of the Registrant's common stock on March 9, 2026 as reported on the New York Stock Exchange multiplied by 85%, which is the percentage of the price per share applicable to purchases under the 2024 ESPP.