<?xml version="1.0" encoding="utf-8"?>
<!--XBRL document created with Toppan Merrill Bridge  11.2.0.5 -->
<!--Based on XBRL 2.1-->
<!--Created on: 3/9/2026 3:42:23 PM-->
<!--Modified on: 3/9/2026 3:42:23 PM-->
<link:linkbase xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" />
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" />
  <link:arcroleRef arcroleURI="http://www.esma.europa.eu/xbrl/esef/arcrole/wider-narrower" xlink:type="simple" xlink:href="http://www.xbrl.org/lrr/arcrole/esma-arcrole-2018-11-21.xsd#wider-narrower" />
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#IndividualsOnly" />
  <link:roleRef roleURI="http://xbrl.sec.gov/ecd/role/PvpAdjustmentsOnly" xlink:type="simple" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#PvpAdjustmentsOnly" />
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/role/PvpAdjustmentsOnly" xlink:type="extended" xlink:title="Pay vs Performance Adjustments">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#ecd_AggtPnsnAdjsSvcCstMember" xlink:label="ecd_AggtPnsnAdjsSvcCstMember" xlink:title="ecd_AggtPnsnAdjsSvcCstMember" />
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#ecd_AggtChngPnsnValInSummryCompstnTblForAplblYrMember" xlink:label="ecd_AggtChngPnsnValInSummryCompstnTblForAplblYrMember" xlink:title="ecd_AggtChngPnsnValInSummryCompstnTblForAplblYrMember" />
  </link:definitionLink>
  <link:definitionLink xlink:role="http://xbrl.sec.gov/ecd/role/IndividualsOnly" xlink:type="extended" xlink:title="Individuals">
    <link:loc xlink:type="locator" xlink:href="https://xbrl.sec.gov/ecd/2025/ecd-2025.xsd#ecd_AllIndividualsMember" xlink:label="ecd_AllIndividualsMember" xlink:title="ecd_AllIndividualsMember" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_GonzalezMember" xlink:label="abbv_GonzalezMember" xlink:title="abbv_GonzalezMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_GonzalezMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_GonzalezMember" order="2" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_MichaelMember" xlink:label="abbv_MichaelMember" xlink:title="abbv_MichaelMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_MichaelMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_MichaelMember" order="1" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_ScottT.ReentsMember" xlink:label="abbv_ScottT.ReentsMember" xlink:title="abbv_ScottT.ReentsMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_ScottT.ReentsMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_ScottT.ReentsMember" order="4" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_JeffreyR.StewartMember" xlink:label="abbv_JeffreyR.StewartMember" xlink:title="abbv_JeffreyR.StewartMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_JeffreyR.StewartMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_JeffreyR.StewartMember" order="5" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_AzitaSalekiGerhardtMember" xlink:label="abbv_AzitaSalekiGerhardtMember" xlink:title="abbv_AzitaSalekiGerhardtMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_AzitaSalekiGerhardtMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_AzitaSalekiGerhardtMember" order="6" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_TimothyJ.RichmondMember" xlink:label="abbv_TimothyJ.RichmondMember" xlink:title="abbv_TimothyJ.RichmondMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_TimothyJ.RichmondMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_TimothyJ.RichmondMember" order="8" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_RoopalB.ThakkarMember" xlink:label="abbv_RoopalB.ThakkarMember" xlink:title="abbv_RoopalB.ThakkarMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_RoopalB.ThakkarMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_RoopalB.ThakkarMember" order="7" priority="0" />
    <link:loc xlink:type="locator" xlink:href="abbv-20260309.xsd#abbv_RobertA.MichaelMember" xlink:label="abbv_RobertA.MichaelMember" xlink:title="abbv_RobertA.MichaelMember" />
    <link:definitionArc xlink:type="arc" xlink:from="ecd_AllIndividualsMember" xlink:to="abbv_RobertA.MichaelMember" use="optional" xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:title="definition:ecd_AllIndividualsMember To abbv_RobertA.MichaelMember" order="3" priority="0" />
  </link:definitionLink>
</link:linkbase>
