S-8 S-8 EX-FILING FEES 0001980845 enGene Holdings Inc. N/A Fees to be Paid Fees to be Paid Fees to be Paid Fees to be Paid 0001980845 2025-09-10 2025-09-10 0001980845 1 2025-09-10 2025-09-10 0001980845 2 2025-09-10 2025-09-10 0001980845 3 2025-09-10 2025-09-10 0001980845 4 2025-09-10 2025-09-10 iso4217:USD xbrli:pure xbrli:shares

Calculation of Filing Fee Tables

S-8

enGene Holdings Inc.

Table 1: Newly Registered Securities

Security Type

Security Class Title

Fee Calculation Rule

Amount Registered

Proposed Maximum Offering Price Per Unit

Maximum Aggregate Offering Price

Fee Rate

Amount of Registration Fee

1 Equity Common Shares, no par value per share Other 2,000,000 $ 5.35 $ 10,700,000.00 0.0001531 $ 1,638.17
2 Equity Common Shares, no par value per share Other 144,650 $ 4.44 $ 642,246.00 0.0001531 $ 98.33
3 Equity Common Shares, no par value per share Other 416,250 $ 3.31 $ 1,377,787.50 0.0001531 $ 210.94
4 Equity Common Shares, no par value per share Other 93,200 $ 3.79 $ 353,228.00 0.0001531 $ 54.08

Total Offering Amounts:

$ 13,073,261.50

$ 2,001.52

Total Fee Offsets:

$ 0.00

Net Fee Due:

$ 2,001.52

Offering Note

1

(a) Pursuant to Rule 416 under the Securities Act of 1933, as amended (the "Securities Act"), the registrant is also registering an indeterminate number of additional common shares, without par value (the "Common Shares") that may become issuable as a result of any stock dividend, stock split, recapitalization or other similar transaction. (b) Consists of 2,000,000 Common Shares issuable pursuant to the enGene Holdings Inc. 2025 Employee Stock Purchase Plan (the "ESPP") approved by the Registrant's shareholders on June 10, 2025 at its 2025 Annual General Meeting of shareholders. (c) Estimated in accordance with Rule 457(c) and Rule 457(h) promulgated under the Securities Act solely for the purpose of calculating the registration fee based on $ 5.35, which is the average of the high and low prices of the Common Shares as reported on the Nasdaq Capital Market on September 9, 2025. Pursuant to the ESPP, the purchase price per each share of common stock reserved for issuance thereunder will be eighty-five percent (85%) of the lower of (i) the Fair Market Value per Share on the Offering Date of the applicable Purchase Period or (ii) the Fair Market Value per Share on the Purchase Date of the applicable Purchase Period (as such terms are defined in the ESPP).

2

(a) Pursuant to Rule 416 under the Securities Act of 1933, as amended (the "Securities Act"), the registrant is also registering an indeterminate number of additional common shares, without par value (the "Common Shares") that may become issuable as a result of any stock dividend, stock split, recapitalization or other similar transaction. (b) Represents the maximum number of Common Shares issuable pursuant to the inducement stock option awards made pursuant to and in accordance with the inducement grant exception under Nasdaq Listing Rule 5635(c)(4). (c) Estimated in accordance with Rule 457(h) promulgated under the Securities Act solely for the purpose of calculating the registration fee based upon the exercise price of the inducement options.

3

See Offering Note 2(a), 2(b) and 2(c).

4

See Offering Note 2(a), 2(b) and 2(c).
Table 2: Fee Offset Claims and Sources ☑Not Applicable
Registrant or Filer Name Form or Filing Type File Number Initial Filing Date Filing Date Fee Offset Claimed Security Type Associated with Fee Offset Claimed Security Title Associated with Fee Offset Claimed Unsold Securities Associated with Fee Offset Claimed Unsold Aggregate Offering Amount Associated with Fee Offset Claimed Fee Paid with Fee Offset Source
Rule 457(p)
Fee Offset Claims N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Fee Offset Sources N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A