Corporate Presentation August 2025 | Soleno Therapeutics Exhibit 99.1

| © 2025 Soleno Therapeutics Certain Notices and Disclaimers Forward-Looking Statements This presentation contains forward-looking statements that are subject to many risks and uncertainties. Forward-looking statements appear in a number of places throughout this presentation and include statements regarding our intentions, beliefs, projections, outlook, analyses or current expectations concerning, among other things, our ongoing and planned product development and clinical trials; the timing of, and our ability to make, regulatory filings and obtain and maintain regulatory approvals for our product candidates; our intellectual property position; the degree of clinical utility of our products, particularly in specific patient populations; our expectations regarding adverse events and discontinuation rates; our ability to develop commercial functions; expectations regarding product launch and revenue; our results of operations and cash needs; financial condition, liquidity, prospects, growth and strategies; the industry in which we operate; and the trends that may affect the industry or us. We may, in some cases, use terms such as “believes,” “estimates,” “anticipates,” “expects,” “plans,” “intends,” “may,” “could,” “might,” “will,” “should,” “approximately” or other words that convey uncertainty of future events or outcomes to identify these forward-looking statements. Although we believe that we have a reasonable basis for each forward-looking statement contained in this presentation, we caution you that forward-looking statements are not guarantees of future performance and that our actual results of operations, financial condition and liquidity, and the development of the industry in which we operate may differ materially from the forward-looking statements contained in this presentation. You should also read carefully the factors described in the “Risk Factors” sections and other parts of our Annual Reports on Form 10-K and Quarterly Reports on Form 10-Q, available at www.sec.gov, in order to better understand the risks and uncertainties inherent in our business and underlying any forward-looking statements. As a result of these factors, we cannot assure you that the forward-looking statements in this presentation will prove to be accurate. Furthermore, if our forward-looking statements prove to be inaccurate, the inaccuracy may be material. In light of the significant uncertainties in these forward-looking statements, you should not regard these statements as a representation or warranty by us or any other person that we will achieve our objectives and plans in any specified timeframe, or at all. Any forward-looking statements that we make in this presentation speak only as of the date of such statement, and we undertake no obligation to update such statements to reflect events or circumstances after the date of this presentation or to reflect the occurrence of unanticipated events. Not for Product Promotional Use

Soleno Therapeutics (NASDAQ: SLNO) | © 2025 Soleno Therapeutics Strategic Highlights Protected by multiple layers of granted and pending patents Provides composition of matter protection, as well as protection of formulations and method of use Potential for substantial patent term extension to mid- late 2030s Significant IP protection Orphan designation in US and EU. Breakthrough and Fast Track granted in US Significant upside potential in other indications Orphan designation granted for GSD1a in US Breakthrough, Orphan and Fast Track Designations Strong balance sheet >$2B US PWS market opportunity Addresses the defining symptom of PWS Significant commercial potential in PWS, an orphan indication with high unmet need. VYKAT XR first approved treatment for hyperphagia, the defining symptom of PWS VYKATTM XR approved for treatment of hyperphagia in patients with PWS 4 yrs and older Approved March 26, 2025 Clinical Trial met primary endpoint with significant differences in hyperphagia Decades-long safety profile of parent molecule Cash runway extends beyond launch of VYKAT XR June 2025 pro forma cash, cash equivalents and marketable securities ~$510m1 Sufficient to fund Company well into commercial launch PWS, Prader-Willi syndrome Not for Product Promotional Use 1Includes ~$216m of net proceeds from July 2025 CMPO

Soleno proprietary quant research Butler MG, et al., Genet Med. 2017 Jun;19(6):635-642. Kayadjanian N et al., PLoS One 2018 Mar 26; 12(3): e0194655 Global survey conducted by the Foundation for Prader-Willi Research Mazaheri MM, et al., J Intellect Disabil Res. 2013 Sep;57(9):861-73. Prader-Willi Syndrome: A Complex Rare, Genetic Neurobehavioral, Metabolic Disorder with Dire Unmet Needs | © 2025 Soleno Therapeutics Disease Overview Burden of Disease Highest Unmet Needs Due to loss or lack of expression of genes on chromosome 15 Birth incidence  ~1:15,000, diagnosed around birth in most cases Characteristics: Hyperphagia, significant behavioral problems, low IQ, low muscle mass, scoliosis High mortality rates with mean age of death ~30 years2 but with many now living into the 50s or longer Hyperphagia, an insatiable desire to eat, is present in virtually all patients with PWS1,4 Disruptive PWS-related behaviors food and non-food related (e.g. significant aggression leading to ER visits) Abnormal body composition with low muscle mass and high fat mass4 People with PWS require supervised care for life1 with children typically living with families and adults often in group homes Constant monitoring and creation of food secure zones greatly interfere with activities of daily life Caregiver burden is highest after onset of hyperphagia; higher than those measured in caregivers for persons with Alzheimer’s3 92% of the siblings indicated moderate-to-severe PTSD5

| © 2025 Soleno Therapeutics FDA APPROVED MARCH 26, 2025 Indicated for the treatment of hyperphagia in adults and pediatric patients 4 years of age and older with Prader-Willi syndrome (PWS) Not for Product Promotional Use

Changing What it Means to Live with PWS | © 2025 Soleno Therapeutics VYKAT XR is the first-to-market treatment for hyperphagia in patients with PWS 4 yrs and older Clinical program demonstrated ability to significantly reduce hyperphagia and impact other PWS-related comorbidities VYKAT XR can become the foundational therapy for patients with PWS Not for Product Promotional Use

DCCR Phase 3 Clinical Program | © 2025 Soleno Therapeutics C601 13-Week Double-Blind Treatment C601 13-Week Double-blind Treatment C602 C602 C614 Open-Label Extension Open-Label Extension Period 16-week Randomized Withdrawal Period 2:1 Randomization N = 127 DCCR N = 85 Placebo N = 42 Completed n = 120 Early Termination n = 7 Enrolled N = 115 As of October 2022 N = 83 1:1 Randomization Oct 2022 - May 2023 N = 77 DCCR N = 38 Placebo N = 39 Enrolled N = 77 Not for Product Promotional Use

HQ-CT Total Scores Over Time1, 2 Studies C601, C602-OLE, C602-RWP, and C614 HQ-CT, Hyperphagia Questionnaire for Clinical Trials; OLE, open-label extension; RWP, randomized withdrawal period 1.  Gevers E. Long term efficacy of Diazoxide Choline in participants with PWS from the completed C601 (Destiny PWS) and C602 (OLE). Oral Presentation at European Society of Pediatric Endocrinology Meeting, Nov. 2024; Liverpool, England. 2. Data on File, Soleno Therapeutics, 2025. Mean (95% CI) HQ-CT Total Score (0-36) C602-RWP Controlled C601 Controlled Time (Week) 36 C602-OLE Open-label Re-randomization 0 13 26 52 N=124 (DCCR 82, Placebo 42) N=115 N=77 (DCCR 38, Placebo 39) N=77 0 4 8 12 16 BL 4 8 13 13 26 39 52 78 104 130 156 DCCR Placebo C601 Inclusion Criteria (HQ-CT ≥ 13) Not for Product Promotional Use

DCCR Safety Profile* Extensive clinical trial safety database with >100 PWS patients treated >1 year >440 total person-years of experience overall Includes patients with >6 years of continuous exposure Safety profile generally consistent with prior experience with DCCR and the known profile of diazoxide The most common adverse events reported were hypertrichosis, edema, and hyperglycemia Typically self-limiting, some needing dose adjustment, interruption, or other treatment (e.g., oral antidiabetics for hyperglycemia or diuretics for edema) Notable AEs included serious AEs of erythema multiforme & diabetic ketoacidosis (1 event each) | © 2025 Soleno Therapeutics Not for Product Promotional Use * AEs reported in DCCR Clinical Trial Program and described in the VYKAT XR US Package Insert Glycemic Outcomes of Diazoxide Choline Extended-Release (DCCR) Tablets Administered for Hyperphagia in Individuals with Prader-Willi Syndrome Over 4 Years; ENDO July 2025 Long-term Administration of Diazoxide Choline Extended-Release Tablet (DCCR); ENDO July 2025 (Available at https://investors.soleno.life/presentations/scientific-posters-presentations)

Scientific Outreach & Community Engagement | © 2025 Soleno Therapeutics Continued engagement with PWS community, HCPs and PAOs Growing body evidence presented at medical and scientific conferences by key opinion leaders and study physicians Engage and support the PWS community by attending local, state and national patient advocacy meetings, conferences and events Created a Community Council that has 20 members of the PWS community of which 3 are adults with PWS – members provide feedback on initiatives, programs and materials Partnering with PWSA|USA to support the PWS community’s advocacy such as those related to Medicaid and recognition of PWS as a disability Not for Product Promotional Use HCP, Healthcare Professionals; PAO, Patient Advocacy Organization

VYKAT XR Indication Presents a Significant Commercial Opportunity | © 2025 Soleno Therapeutics U.S. PWS Birth Incidence Verified in Claims: Treated People Living With PWS Total Addressable Market (TAM) Diagnosis Rate ~85% of diagnoses are made within the first year of life1 Total On-label Addressable Market Estimated ~10,000 people with PWS 4 years of age and older with hyperphagia and no exclusionary comorbidities ~300 HCPs are primary treaters of ~2,100 PWS patients and influence treatment decisions for an additional ~2,000 patients2 Not for Product Promotional Use Wheeler, A.C., Gantz, M.G., Cope, H. et al. J Neurodevelop Disord 15, 37 (2023) ICD10 claims data – Soleno purchased data

Pathway to Successful US Launch of VYKAT XR | © 2025 Soleno Therapeutics Comprehensive Access Strategy Rare Disease & Launch Capabilities Differentiated formulation Efficacy observed in multiple aspects of the disease in clinical trials ~5 years of response in clinical trial data Well characterized response profile Invested in analytics to map TAM Account profiling to define influence and catchment areas Field Force teams with deep rare disease and launch experience Mapped payer mix to support rapid uptake Educating payers on value proposition Distribution partners with extensive rare disease experience Stakeholder Engagement Deep community and advocacy engagement Launched Disease State Education programs www.support4PWS.com Strong presence at medical congresses Not for Product Promotional Use Robust Clinical Program

Soleno proprietary quant research ICD10 claims data Launch Strategy Requires Specific Considerations for Different Age Groups | © 2025 Soleno Therapeutics Onset of hyperphagia and increasing disruptive PWS-related behaviors Caregivers and families are actively engaged in care (~4 visits/year)1 Majority live with family, with support from schools Pediatric Endocrinologists are primary point of care, with support from multiple specialties Young patients (4 to 25 years old) 01 Transition to adult care disrupts continuity of care, coincides with increased desire for independence Lack of independence; reliant on food-security and 24/7 monitoring Majority of adults still live with family members, with ~20% individuals living in residential programs2 Adult Endocrinologists are the primary treaters, mostly focused on mitigating health deterioration Adult patients (>25 years old) 02 Not for Product Promotional Use

Broad Reimbursement Supported by Clear Clinical Value Proposition for VYKAT XR | © 2025 Soleno Therapeutics Communicate compelling value proposition to gain payer coverage Urgent need for hyperphagia treatment: severe burden and high mortality rate Rare disease with small patient numbers leading to low budget impact Robust and durable clinical data supporting significant effect on hyperphagia with VYKAT XR VYKAT XR is the first and only FDA approved medication for treatment of hyperphagia in people with PWS Not for Product Promotional Use

Soleno OneTM Provides End-to-End Patient Support | © 2025 Soleno Therapeutics Limited Distribution Model Soleno OneTM live Day 1 of Launch First prescription sent by April 14th 1-833-SOLENO-1 Access. Education. Resources. Not for Product Promotional Use

| © 2025 Soleno Therapeutics First and only approved treatment for hyperphagia in individuals 4 years of age and older with PWS Approval based on comprehensive clinical trial program Key elements of VYKAT XR label No exclusions for severity of hyperphagia No Boxed Warning No Risk Evaluation and Mitigation Strategy No contraindications VYKAT XR should not be substituted with diazoxide oral suspension Available in the U.S. as of April 10, 2025 Positioned To Be The Standard of Care Once daily oral dosing Not for Product Promotional Use

Received 646 start forms Number of new prescribers: 295 ~100 million Lives Covered 1 2 3 Launch to End of Q2 2025 Performance Metrics Not for Product Promotional Use Launch Metrics as of End of Day June 30, 2025 | © 2025 Soleno Therapeutics 5.2 % adverse event related discontinuations as of August 15, 2025

Significant Opportunity in Europe Confirmed high unmet need Strong thought leader support Concentrated market driven by centers of excellence Estimated ~9,500 people living with PWS in EU4 and UK1 In May 2025, we announced submission and EMA validation of MAA | © 2025 Soleno Therapeutics United Kingdom France Luxembourg Belgium Ireland Netherlands Germany Denmark Norway Sweden Latvia Lithuania Belarus Russia Ukraine Poland Czecb Republic Slovakia Moldova Romania Hungary Austria Liechenstein Switzerland Monaco Portugal Andorra Spain Turkey Bulgaria San Marino Italy Vatican City Serbia Slovenia Croatia Bosnia and Herzegovina Kosovo North Macedonia Montenegro Albania Greece Algeria Tunisia Malta 1 1 [100 to 200[ [0 to 100[ [300 to 400[ [200 to 300[ 400+ 400+ 400+ 400+ 400+ 400+ Orpha Net Birth Prevalence of 1/22.5k Not for Product Promotional Use

Extensive IP Protection | © 2025 Soleno Therapeutics Three families of patents prosecuted in major pharma markets – primary cases in all three issued Uses of pharmaceutical formulations of KATP channel activators PWS relevant claims: treatment of hyperphagia in PWS with diazoxide 20-Year Expiration 8/2025 Salts of KATP channel activators and uses thereof PWS relevant claims: composition of matter (salt and polymorph), formulation, method of manufacture, methods to treat overweight, obese and obesity prone individuals 5 US patents 20-Year expiration12/2026 Potential expiration w/PTA 3/2029 Potential expiration w/PTA & PTE 2034 Methods to treat PWS Patients Specific claims to behavioral, body composition, and cardiometabolic marker changes in response to treatment with DCCR, diazoxide or KATP channel activators, dependent claims to treating hyperphagia 4 US patents + 1 application 20-Year expiration 11/2035 Potential expiration w/PTE 2038/2039 Not for Product Promotional Use

Financial Highlights Fully Diluted Share Count June 30, 2025 Cash in millions Pro forma cash1 $509.5 Debt $50.0 Cash, cash equivalents and investments 1 Includes marketable securities and $215.7m from July 2025 public offering June 30, 2025 In millions Common stock 50.4 Pre-funded warrants 0.3 March 2022 warrants – $4.50 0.5 Options and RSUs 3.9 July 2025 public offering 2.7 Pro Forma Total 57.8 | © 2025 Soleno Therapeutics Not for Product Promotional Use

Corporate Presentation August 2025| Soleno Therapeutics