v3.25.1
Cover Page - shares
 3 Months Ended
Mar. 31, 2025
Apr. 24, 2025
Cover [Abstract]    
Document Type 10-Q  
Document Quarterly Report true  
Document Period End Date Mar. 31, 2025  
Document Transition Report false  
Entity File Number 001-39434  
Entity Registrant Name NAUTILUS BIOTECHNOLOGY, INC.  
Entity Incorporation, State or Country Code DE  
Entity Tax Identification Number 98-1541723  
Entity Address, Address Line One 2701 Eastlake Avenue East  
Entity Address, City or Town Seattle  
Entity Address, State or Province WA  
Entity Address, Postal Zip Code 98102  
City Area Code 206  
Local Phone Number 333-2001  
Title of 12(b) Security Common Stock, par value $0.0001 per share  
Trading Symbol NAUT  
Security Exchange Name NASDAQ  
Entity Current Reporting Status Yes  
Entity Interactive Data Current Yes  
Entity Filer Category Non-accelerated Filer  
Entity Small Business true  
Entity Emerging Growth Company true  
Entity Ex Transition Period false  
Entity Shell Company false  
Entity Common Stock, Shares Outstanding   126,148,469
Entity Central Index Key 0001808805  
Amendment Flag false  
Current Fiscal Year End Date --12-31  
Document Fiscal Year Focus 2025  
Document Fiscal Period Focus Q1